General Information

    • DRAMP ID

    • DRAMP29084

    • Peptide Name

    • Brevinin-GR23

    • Source

    • Hylarana guentheri

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • GVTFNALKGVAKTVAAQLLKTAR

    • Sequence Length

    • 23

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-, Anti-Gram+

    • Target Organism

      • [Ref.31549575] Gram-positive bacteria: Staphylococcus aureus (MIC = 16 μM), Streptococcus mutans(MIC > 100 μM);
      • Gram-negative bacteria: Escherichia coli (MIC = 64 μM), Pseudomonas aeruginosa(MIC > 100 μM);
      • Yeast: Canidia albican (MIC > 100 μM)

    • Hemolytic Activity

      • [Ref.31549575]B-GR23 induced a hemolysis of 0.13%, 0.23%, 0.38%, 0.75%, 0.83% and 1.60% on human blood red cells at the concentrations of 25, 50, 75, 100, 150 and 200 μM.

    • Cytotoxicity

      • Not found

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helical

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29084 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29084.

Physicochemical Information

    • Formula

    • C106H185N31O29

    • Absent Amino Acids

    • CDEHIMPSWY

    • Common Amino Acids

    • A

    • Mass

    • 2357.83

    • PI

    • 11.26

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +4

    • Boman Index

    • -1067

    • Hydrophobicity

    • 0.422

    • Aliphatic Index

    • 110.43

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 6

DRAMP29084

DRAMP29084 chydropathy plot

Comments Information

    • Comment

    • Although B-GR23 did not show a potent antimicrobial and antibiofilm activity, the outstanding characteristic is the low toxicity on human blood red cells, which makes it an ideal template peptide to derive more powerful antimicrobial peptides

Literature Information

  • ·Literature 1

    • Title

    • Brevinin-GR23 from frog Hylarana guentheri with antimicrobial and antibiofilm activities against Staphylococcus aureus

    • Reference

    • Biosci Biotechnol Biochem. 2020 Jan;84(1):143-153. doi: 10.1080/09168451.2019.1670045. Epub 2019 Sep 24.

    • Author

    • Hengren Zhong, Zhipeng Xie, Shuxia Zhang, Hanqi Wei, Yanting Song, Yingxia Zhang, Manchuriga Wang