• • DRAMP ID

  • DRAMP29087

• • Peptide Name

  • RiLK1

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • N/A

• • Sequence

  • VRLIVKVRIWRR

• • Sequence Length

  • 12

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.32971824] Gram-negative bacteria: E.coli (IC50 = 1.20 ± 0.10 μM, MBC = 2.0 μM), S. Typhimurium (IC50 = 1.30 ± 0.14 μM, MBC = 2.5 μM);
    • Gram-positive bacteria: S. aureus (IC50 = 1.98 ± 0.25 μM, MBC = 16.0 μM), L. monocytogenes (IC50 = 0.46 ± 0.01 μM, MBC = 2.0 μM);
    • Fungi: C. albicans ATCC 14053, A. brasiliensis ATCC 9341. Susceptibility testing clearly showed that RiLK1 was very effective, being able to inhibit 100% growth of both fungi at 25 μM concentration (MFC).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • [Ref.32971824] It was observed that the cell viability was recorded as 98%, 98.7%, and 98.9% at the concentrations of RiLK1 (10, 25, 50 μM)

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Disordered structure in aqueous solution; β-sheet in the presence of SDS (3 mM)

• • Structure Description

  • Without SDS: 1.2% α-helix, 47.3% β-sheet, 51.5% random; t = 0 h with SDS: 7.4% α-helix, 51.4% β-sheet, 41.3% random; t = 5 min with SDS: 4.4% α-helix, 53.4% β-sheet, 42.2% random; t = 30 min with SDS: 11% α-helix, 48.7% β-sheet, 40.3% random; t = 1 h with SDS: 9.8% α-helix, 51.1% β-sheet, 39.1% random; t = 5 h with SDS: 6.7% α-helix, 54% β-sheet, 39.3% random; t = 6 h with SDS: 8% α-helix, 52% β-sheet, 40.1% random; t = 24 h with SDS: 8.4% α-helix, 53.6% β-sheet, 38% random

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29087.

DRAMP29087

• • Comment

  • RiLK1 exerted potent killing effects against the clinical isolate (MBC = 1.25 M), two-fold lower than those observed against the strain isolated from food products and those determined with the parental peptide 1018-K6 against ACSSuT strain, thus, suggesting that RiLK1 might have a high potential also in the medical field.

• ·Literature 1

• • Title

  • A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization

• • Pubmed ID

  • 32971824

• • Reference

  • Int J Mol Sci. 2020 Sep 22;21(18):6952. doi: 10.3390/ijms21186952.

• • Author

  • Agrillo B, Proroga YTR, Gogliettino M, Balestrieri M, Tatè R, Nicolais L, Palmieri G