General Information

    • DRAMP ID

    • DRAMP29090

    • Peptide Name

    • MSI-1

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • GIWKFLKKAKKFWK

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal, Anti-cryptococcal

    • Target Organism

      • [Ref.32504765] Fungal strains: C. neoformans ATCC4906 (MIC = 8 μg/mL), C. neoformans 1201 (MIC = 8 μg/mL), C. neoformans 1768 (MIC = 16 μg/mL), C. neoformans 2011 (MIC = 8 μg/mL), C. neoformans 1386 (MIC = 16 μg/mL), C. neoformans 1965 (MIC = 16 μg/mL), C. gattii ATCC34877 (MIC = 4 μg/mL), C. albicans ATCC10231 (MIC = 32 μg/mL), C. albicans 2713 (MIC = 32 μg/mL), C. albicans 2144 (MIC = 64 μg/mL), C. albicans 1902 (MIC = 64 μg/mL), C. albicans 1869 (MIC = 32 μg/mL), C. albicans 2105 (MIC = 128 μg/mL )

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not found

    • Binding Target

    • Fungal cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helical in hydrophobic environment

    • Structure Description

    • Furthermore, the amphipathic structure of this peptide makes it adopt an alpha helical in hydrophobic environment which may lead to the insertion of MSI-1 into the lipid bilayer of the fungal membrane to exert antifungal activity.

    • Helical Wheel Diagram

    • DRAMP29090 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29090.

Physicochemical Information

    • Formula

    • C93H142N22O15

    • Absent Amino Acids

    • CDEHMNPQRSTVY

    • Common Amino Acids

    • K

    • Mass

    • 1808.29

    • PI

    • 10.7

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +6

    • Boman Index

    • -1009

    • Hydrophobicity

    • -0.707

    • Aliphatic Index

    • 62.86

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 846.15

    • Polar Residues

    • 1

DRAMP29090

Comments Information

    • Comment

    • The study had demonstrated the potent antifungal activity of MSI-1 against clinical isolated C. neoformans in vitro and in a murine model of cryptococcal meningoencephalitis.

Literature Information

  • ·Literature 1

    • Title

    • Antifungal activity of peptide MSI-1 against Cryptococcus neoformans infection in vitro and in murine cryptococcal meningoencephalitis

    • Reference

    • Peptides. 2020 Aug;130:170334. doi: 10.1016/j.peptides.2020.170334. Epub 2020 Jun 3.

    • Author

    • Ma L, Wei S, Ye X, Xu P, Chen H, Liu Z, Zhou C