• DRAMP ID

    • DRAMP29141
    • Peptide Name

    • Pxt‐3
    • Source

    • Xenopus tropicalis
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • GLKEVAHSAKKFAKGFISGLTGS
    • Sequence Length

    • 23
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Not found
    • Biological Activity

    • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-, Antifungal
    • Target Organism

      • [Ref.25312021]Gram-negative bacteria:Escherichia coli DH5α(Not active up to 150 µg/ml);
      • Gram-positive bacteria:Staphylococcus aureus JCM 2151(Not active up to 150 µg/ml).
      • [Ref.23935531]Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=64 µm);Pseudomonas aeruginosa ATCC 15692(MIC >256 µM);
      • Gram-positive bacteria:Staphylococcus aureus ATCC 25923(MIC >256 µM);Micrococcus luteus(MIC=32 µM);
      • Fungi:Saccharomyces cerevisiae(MIC=128 µM);
      • Parasite:Trypanosoma brucei(LC95=8->256 µM).
    • Hemolytic Activity

      • [Ref.25312021]0.2% Hemolysis against Rat erythrocytes at 20 µM.
      • [Ref.23935531] Hemolysis against Mouse erythrocytes (HC50>256 µm).
    • Cytotoxicity

      • [Ref.23935531]Cytotoxicity against T-lymphocytes(IC50>256 µM).
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Free
    • Nonterminal Modifications and Unusual Amino Acids

    • None
    • Stereochemistry

    • L
    • Structure

    • Alpha helix,random coil
    • Structure Description

    • 0.5%,1.2%,2.0% α-helix were mearsured by CD spectra at 10 °C, 25 °C and 37 °C in 10 mm Tris-HCl (pH 7.2).
    • Helical Wheel Diagram

    • DRAMP29141 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP29141.
    • Formula

    • C106H173N29O30
    • Absent Amino Acids

    • CDMNPQRWY
    • Common Amino Acids

    • GK
    • Mass

    • 2333.72
    • PI

    • 10
    • Basic Residues

    • 5
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 9
    • Net Charge

    • +4
    • Boman Index

    • -1249
    • Hydrophobicity

    • 0.013
    • Aliphatic Index

    • 76.52
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 8

DRAMP29141

DRAMP29141 chydropathy plot
    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.
  • ·Literature 1
    • Title

    • Identification of novel peptides from amphibian (Xenopus tropicalis) skin by direct tissue MALDI-MS analysis.
    • Reference

    • FEBS J. 2015 Jan;282(1):102-13.
    • Author

    • Shigeri Y, Yasuda A, Hagihara Y, Nishi K, Watanabe K, Imura T, Inagaki H, Haramoto Y, Ito Y, Asashima M.
  • ·Literature 2
    • Title

    • Origin and functional diversification of an amphibian defense peptide arsenal.
    • Reference

    • PLoS Genet. 2013;9(8):e1003662.
    • Author

    • Roelants K, Fry BG, Ye L, Stijlemans B, Brys L, Kok P, Clynen E, Schoofs L, Cornelis P, Bossuyt F.