General Information

    • DRAMP ID

    • DRAMP29147

    • Peptide Name

    • Pxt‐12(CPF-St7)

    • Source

    • Xenopus tropicalis

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • NLLGSLLKTGLKVGSNLL

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-,Antifungal

    • Target Organism

      • [Ref.26802742]Gram-negative bacteria:Escherichia coli NBRC 14237(MIC<12.5 µM);Pseudomonas aeruginosa NBRC 12582(MIC<50 µM);
      • Gram-positive bacteria:Staphylococcus aureus NBRC 12732(MIC<12.5 µM);Micrococcus luteus NBRC 12708(MIC<50 µM);
      • Fungi:Candida albicans NBRC 1594(ND).
      • [Ref.23935531]Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=128 µM);Pseudomonas aeruginosa ATCC 15692(MIC >256 µM);
      • Gram-positive bacteria:Staphylococcus aureus ATCC 25923(MIC=32 µM);Micrococcus luteus(MIC=2 µM);
      • Fungi:Saccharomyces cerevisiae(MIC=64 µM);
      • Parasite:Trypanosoma brucei(LC95=2-64 µM).

    • Hemolytic Activity

      • [Ref.26802742]0% Hemolysis against Rat erythrocytes at 50 µM.
      • [Ref.23935531]Hemolysis against Mouse erythrocytes(IC50=256 µM).

    • Cytotoxicity

      • [Ref.23935531]Cytotoxicity against T-lymphocytes(IC50=128 µM).

    • Binding Target

    • liposomes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix,random coil

    • Structure Description

    • 0%,2.5%,6.6% α-helix were mearsured by CD spectra at 10 °C, 25 °C and 37 °C in 10 mm Tris-HCl (pH 7.2);68.2%,57.4%,50.5% α-helix were mearsured by CD spectra at 10 °C, 25 °C and 37 °C in 50% TFE.

    • Helical Wheel Diagram

    • DRAMP29147 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29147.

Physicochemical Information

    • Formula

    • C83H150N22O24

    • Absent Amino Acids

    • ACDEFHIMPQRWY

    • Common Amino Acids

    • L

    • Mass

    • 1840.24

    • PI

    • 10

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • 755

    • Hydrophobicity

    • 0.694

    • Aliphatic Index

    • 167.78

    • Half Life

      • Mammalian:1.4 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 8

DRAMP29147

DRAMP29147 chydropathy plot

Comments Information

    • Induced a significant and dose-dependent histamine release.

Literature Information

  • ·Literature 1

    • Title

    • Physicochemical and biological characterizations of Pxt peptides from amphibian (Xenopus tropicalis) skin.

    • Reference

    • J Biochem. 2016 Jun;159(6):619-29.

    • Author

    • Shigeri Y, Horie M, Yoshida T, Hagihara Y, Imura T, Inagaki H, Haramoto Y, Ito Y, Asashima M.
  • ·Literature 2

    • Title

    • Origin and functional diversification of an amphibian defense peptide arsenal.

    • Reference

    • PLoS Genet. 2013;9(8):e1003662.

    • Author

    • Roelants K, Fry BG, Ye L, Stijlemans B, Brys L, Kok P, Clynen E, Schoofs L, Cornelis P, Bossuyt F.