General Information
-
DRAMP ID
- DRAMP21548
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Peptide Name
- Mag(i+7)5
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Sequence
- GIGKFⓏHSAKKFⓍKAFVGEIJNS
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Sequence Length
- 23
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Original Sequence
- GIGKFLHSAKKFGKAFVGEIMNS
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Source
- Synthetic construct
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Comments
- Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.
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Target Organism
-
- [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.84 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.13 μg/mL);
- Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.13 μg/mL)
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Hemolytic Activity
-
- [Ref.31427820] It has 82.9% hemolysis against human red blood cells at 25 μg/mL.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ①The Ⓧ (position: 13) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 6) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (13) and Ⓩ (6) are cross-linked by hydrocarbon stapling.
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Stereochemistry
- L
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Secondary Structure
- α-helix in potassium phosphate buffer.
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Structure Description
- No other descriptive information about the structure found in the literature
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Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP21548.
Physicochemical Information
-
Formula
- C₁₀₇H₁₆₈N₂₈O₂₇
Absent Amino Acids
- CDLMPQRTWY
Common Amino Acids
- K
Mass
- 2571.11
PI
- /
-
Basic Residues
- 5
Acidic Residues
- 1
Hydrophobic Residues
- 8
Hydrophobicity
- /
Polar Residues
- 6
DRAMP21548
Literature Information
- Literature 1
-
Title
- Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice
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Pubmed ID
- 31427820
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Reference
- Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.
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Author
- Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky