• DRAMP ID

    • DRAMP29009
    • Peptide Name

    • A4K14-citropin1.1-Sp3
    • Sequence

    • GLFAVⓍKKVⓍSVIKGL
    • Sequence Length

    • 16
    • Original Sequence

    • GLFAVIKKVASVIKGL
    • Source

    • Synthetic construct
    • Biological Activity

    • Antimicrobial, Anticancer
    • Comments

    • Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.
    • Target Organism

      • [Ref.33363118] Cancer cell lines: C4-2B (IC50 = 17.89 μM), A549 (IC50 = 12.11 μM), U87 (IC50 = 11.93 μM), MCF-7 (11.92 μM)
    • Hemolytic Activity

      • No hemolytic activity information found.
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Linear/Cyclic

    • Cyclic (Stapled)
    • N-terminal Modification

    • Acetylation
    • C-terminal Modification

    • Amidation
    • Special Amino Acid and Stapling Position

    • ① The Ⓧ (position: 6 and 10) in sequence indicate (S)-2-(4-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.
    • Stereochemistry

    • L
    • Secondary Structure

    • Helicity = 49.2% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)
    • Structure Description

    • [Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.
    • Helical Wheel Diagram

    • DRAMP29009 helical wheel diagram
    • Predicted Structure

    • There is no predicted structure for DRAMP29009.
    • Formula

    • C₈₆H₁₄₈N₂₀O₁₈
    • Absent Amino Acids

    • CDEHMNPQRTWY
    • Common Amino Acids

    • KV
    • Mass

    • 1749.13
    • PI

    • /
    • Basic Residues

    • 3
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 8
    • Hydrophobicity

    • /
    • Polar Residues

    • 3

DRAMP29009

  • Literature 1
    • Title

    • Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides
    • Reference

    • Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.
    • Author

    • Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu