General Information
-
DRAMP ID
- DRAMP29013
-
Peptide Name
- A4K14-citropin1.1-Sp7
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Sequence
- GLFAVⓏKKVASVⓍKGL
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Sequence Length
- 16
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Original Sequence
- GLFAVIKKVASVIKGL
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Source
- Synthetic construct
Activity Information
-
Biological Activity
- Antimicrobial, Anticancer
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Comments
- Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.
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Target Organism
-
- [Ref.33363118] Cancer cell lines: C4-2B (IC50 = 10.23 μM), A549 (IC50 = 16.37 μM), U87 (IC50 = 14.72 μM), MCF-7 (12.1 μM)
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Hemolytic Activity
-
- No hemolytic activity information found.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Acetylation
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ① The Ⓩ (position: 6) in sequence indicates (R)-2-(7-octenyl)alanine. ② The Ⓧ (position: 13) in sequence indicates (S)-2-(4-pentenyl)alanine. ③ Ⓩ and Ⓧ are cross-linked by ring-closing metathesis through a undec-4-enyl staple.
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Stereochemistry
- L
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Secondary Structure
- Helicity = 13.6% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)
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Structure Description
- [Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.
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Helical Wheel Diagram
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Predicted Structure
- There is no predicted structure for DRAMP29013.
Physicochemical Information
-
Formula
- C₈₆H₁₄₈N₂₀O₁₈
Absent Amino Acids
- CDEHIMNPQRTWY
Common Amino Acids
- KV
Mass
- 1749.13
PI
- /
-
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 8
Hydrophobicity
- /
Polar Residues
- 3
DRAMP29013
Literature Information
- Literature 1
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Title
- Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides
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Pubmed ID
- 33363118
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Reference
- Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.
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Author
- Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu