General Information
-
DRAMP ID
- DRAMP29025
-
Peptide Name
- H-21
-
Sequence
- IKLSKⓍTKKⓍLKKVLKGⓍIKGⓍIAVAKMV
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Sequence Length
- 29
-
Original Sequence
- IKLSPETKDNLKKVLKGAIKGAIAVAKMV
-
Source
- Synthetic construct
Activity Information
-
Biological Activity
- Antimicrobial, Anticancer
-
Comments
- Function: Anticancer activity. Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays
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Target Organism
-
- [Ref.30789695] Cancer cell lines: A549 (IC50 = 3.26 ± 0.36 μM), HCT116 (IC50 = 3.05 ± 0.21 μM), HepG2 (IC50 = 1.50 ± 0.28 μM)
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Hemolytic Activity
-
- No hemolytic activity information found.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ① The Ⓧ (position: 6, 10, 18 and 22) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10), Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple, respectively.
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Stereochemistry
- L
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Secondary Structure
- α-helical (most likely)
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Structure Description
- No detailed structure description found.
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Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP29025.
Physicochemical Information
-
Formula
- C₁₅₅H₂₈₅N₃₉O₃₁S
Absent Amino Acids
- CDEFHNPQRWY
Common Amino Acids
- K
Mass
- 3223.22
PI
- /
-
Basic Residues
- 9
Acidic Residues
- 0
Hydrophobic Residues
- 11
Hydrophobicity
- /
Polar Residues
- 4
DRAMP29025
Literature Information
- Literature 1
-
Title
- Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy
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Pubmed ID
- 30789695
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Reference
- ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.
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Author
- Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao