General Information

    • DRAMP ID

    • DRAMP29026

    • Peptide Name

    • H-57

    • Sequence

    • IKLSKETKKNLKKVLKGⓍIKGⓍIAVAKMV

    • Sequence Length

    • 29

    • Original Sequence

    • IKLSPETKDNLKKVLKGAIKGAIAVAKMV

    • Source

    • Synthetic construct

Activity Information

    • Biological Activity

    • Antimicrobial, Anticancer

    • Comments

    • Function: Anticancer activity. Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays

    • Target Organism

      • [Ref.30789695] Cancer cell lines: A549 (IC50 = 1.10 ± 0.31 μM), HCT116 (IC50 = 1.65 ± 0.28 μM), HepG2 (IC50 = 2.71 ± 0.05 μM)

    • Hemolytic Activity

      • [Ref.30789695] It has -1.2%, 1.8%, 2.8%, 5.5%, 14.5%, 25.8%, 42.8%, 68.8% and 58.2% hemolysis against fresh rabbit blood cells at 0.010, 0.25, 0.5, 1.0, 2.0, 5.0, 10.0, 20.0 and 25.0 μM.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Special Amino Acid and Stapling Position

    • ① The S (position: 4), T (position: 7) and N (position: 10) in sequence are linked with N-acetylglucosamine (GlcNAc), respectively. ② The Ⓧ (position: 18 and 22) indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ③ Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple, respectively.

    • Stereochemistry

    • L

    • Secondary Structure

    • α-helical (most likely)

    • Structure Description

    • No detailed structure description found.

    • Helical Wheel Diagram

    • DRAMP29026 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP29026.

Physicochemical Information

    • Formula

    • C₁₇₄H₃₁₅N₄₃O₄₉S

    • Absent Amino Acids

    • CDFHPQRWY

    • Common Amino Acids

    • K

    • Mass

    • 3825.67

    • PI

    • /

    • Basic Residues

    • 9

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Hydrophobicity

    • /

    • Polar Residues

    • 5

DRAMP29026

Literature Information

  • Literature 1

    • Title

    • Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy

    • Reference

    • ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.

    • Author

    • Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao