General Information
-
DRAMP ID
- DRAMP29238
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Peptide Name
- NYBSP-4
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Sequence
- TIEEQⓏKTFLDKⓍNHEAEDLFYQⓍSLAⓍWN
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Sequence Length
- 30
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Original Sequence
- TIEEQAKTFLDKFNHEAEDLFYQSSLASWN
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Source
- Synthetic construct
Activity Information
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Biological Activity
- Antimicrobial, Antiviral(SARS-CoV-2)
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Comments
- Mechanism of action:Proteolytic stability of NYBSP-4 in human plasma(half-life (T1/2) of NYBSP-4 was >289 min).The stapled peptide designed based on the ACE2 helix, which is expected to bind to SARS-CoV-2 and prevent the binding of the virus to the ACE2 receptor and disrupt the infection.
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Target Organism
-
- [Ref.33310780]Virus:
- SARS-CoV-2:Inhibition of infection in HT1080/ACE2 cells(IC50=1.97 ± 0.14 μM);Inhibition of infection in A549/ACE2 cells(IC50=2.8 ± 0.08 μM);Inhibition of virus-induced cytopathic effect (CPE) in Vero E6 cells(IC100=33 μM);
- VSV-G:Inhibition of infection in HT1080/ACE2 cells(IC50>26.6 μM);Inhibition of infection in A549/ACE2 cells(IC50>26.6 μM).
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Hemolytic Activity
-
- No hemolytic activity information found.
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Cytotoxicity
-
- [Ref.33310780]HT1080/ACE2 cells:CC50>26.6 μM(Less than 10% toxicity at this dose);A549/ACE2 cells:CC50>26.6 μM(Less than 10% toxicity at this dose).
Structure Information
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Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Acylation
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ①The Ⓧ (position: 13,24 and 28) in sequence indicate S-2-(4'-pentenyl) alanine.②The Ⓩ (position: 6) indicates 2-(7'-octenyl) alanine in the R configuration.③ Ⓩ(6) and Ⓧ(13), Ⓧ(24) and Ⓧ(28) are cross-linked by hydrocarbon stapling.
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Stereochemistry
- L
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Secondary Structure
- 80% α-helical content in 10 mM phosphate-buffered saline (PBS)
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Structure Description
- No other descriptive information about the structure found in the literature
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Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP29238.
Physicochemical Information
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Formula
- C161H233N39O53
Absent Amino Acids
- CGMPRV
Common Amino Acids
- E
Mass
- 3562.85
PI
- 4.35
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Basic Residues
- 3
Acidic Residues
- 6
Hydrophobic Residues
- 11
Hydrophobicity
- -74.33
Polar Residues
- 8
DRAMP29238
Literature Information
- Literature 1
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Title
- Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In Vitro.
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Pubmed ID
- 33310780
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Reference
- mBio. 2020 Dec 11;11(6):e02451-20.
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Author
- Curreli F, Victor SMB, Ahmed S, Drelich A, Tong X, Tseng CK, Hillyer CD, Debnath AK.