General Information

    • DRAMP ID

    • DRAMP29241

    • Peptide Name

    • hACE2(21-55)F28K-F32E

    • Sequence

    • IEEQAKTⓀLDKⒺNHEAEDLFYQSSLASWNYNTNIT

    • Sequence Length

    • 35

    • Original Sequence

    • IEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNIT

    • Source

    • Synthetic construct

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral(SARS-CoV-2)

    • Comments

    • Mechanism of action:The stapled peptide inhibit the RBD-hACE2 complex formation, and hACE2 α1-helix-based peptidomimetics could potentially prevent SARS-CoV-2 from entering the human cells through hACE2 and thus inhibit subsequent viral replication.

    • Target Organism

      • [Ref.33651072]Virus:SARS-CoV-2:inhibition of SARS-CoV-2 Spike protein-hACE2 complex formation(IC50=46.8 μM).

    • Hemolytic Activity

      • No hemolytic activity information found.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Special Amino Acid and Stapling Position

    • Ⓚ (8) and Ⓔ (12) are corss-linked by lactam stapling.

    • Stereochemistry

    • L

    • Secondary Structure

    • ①6-13% α-helical content in 10 mM PBS at pH 7.4 and 298 K.②11-52% α-helical content in 10 mM PBS at pH 7.4 with 30% TFE at 298 K.

    • Structure Description

    • Low helicity for stapled hACE2 peptides in absence of TFE, with predictors of helicity averaging to 6-13% helical content. In the presence of TFE, α-helical structures can be observed for various synthetic hACE2 peptides with predictors averaging from 11 to 52% helical content

    • Helical Wheel Diagram

    • DRAMP29241 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP29241.

Physicochemical Information

    • Formula

    • C188H272N46O62

    • Absent Amino Acids

    • CGMPRV

    • Common Amino Acids

    • EN

    • Mass

    • 4168.5

    • PI

    • 4.35

    • Basic Residues

    • 3

    • Acidic Residues

    • 6

    • Hydrophobic Residues

    • 12

    • Hydrophobicity

    • -76.57

    • Polar Residues

    • 12

DRAMP29241

Literature Information

  • Literature 1

    • Title

    • Targeting SARS-CoV-2 spike protein by stapled hACE2 peptides.

    • Reference

    • Chem Commun (Camb). 2021 Apr 4;57(26):3283-3286.

    • Author

    • Maas MN , Hintzen JCJ , Löffler PMG , Mecinović J .