General Information

    • DRAMP ID

    • DRAMP01218

    • Peptide Name

    • Kassinatuerin-1 (Frogs, amphibians, animals)

    • Source

    • Kassina senegalensis (Senegal running frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GFMKYIGPLIPHAVKAISDLI

    • Sequence Length

    • 21

    • Protein Existence

    • Protein level

    • SMILES

    • CC[C@@H](C)[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCSC)NC(=O)[C@H](Cc1ccccc1)NC(=O)CN)[C@H](C)CC)[C@H](C)CC)C(C)C)[C@H](C)CC)C(N)=O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coliMIC=6.25 µM
        Klebsiella pneumoniaeMIC=25 µM
        Enterobacter cloacaeMIC=6.25 µM
        Pseudomonas aeruginosa (MIC=25 µM);-
      • Gram-positive bacteria:
        Target OrganismActivity
        Staphylococcus aureusMIC=6.25 µM
        Staphylococcus epidermidisMIC=6.25 µM
        Enterococcus faecalisMIC=12.5 µM
      • Yeast:
        Target OrganismActivity
        Candida albicansMIC=100 µM

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.15885852]Display cytotoxicity against L929 fibroblasts (LD50=12μM).

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • PDB ID

    • Predicted Structure

    • There is no predicted structure for DRAMP01218.

Physicochemical Information

    • Formula

    • C109H175N25O26S

    • Absent Amino Acids

    • CENQRTW

    • Common Amino Acids

    • I

    • Mass

    • 2283.8

    • PI

    • 8.51

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +2

    • Boman Index

    • 16.37

    • Hydrophobicity

    • 0.833

    • Aliphatic Index

    • 134.76

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 74.5

    • Polar Residues

    • 4

DRAMP01218

Comments Information

    • Function

    • Shows broad-spectrum antimicrobial activity.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

Literature Information

  • ·Literature 1

    • Title

    • Kassinatuerin-1: a peptide with broad-spectrum antimicrobial activity isolated from the skin of the hyperoliid frog, Kassina senegalensis.

    • Reference

    • Biochem Biophys Res Commun. 2000 Feb 16;268(2):433-436.

    • Author

    • Mattute B, Knoop FC, Conlon JM.
  • ·Literature 2

    • Title

    • Antimicrobial and cytolytic properties of the frog skin peptide, kassinatuerin-1 and its L- and D-lysine-substituted derivatives.

    • Reference

    • Peptides. 2005 Nov;26(11):2104-2110.

    • Author

    • Conlon JM, Abraham B, Galadari S, Knoop FC, Sonnevend A, P¡l T.