General Information

    • DRAMP ID

    • DRAMP01445

    • Peptide Name

    • Nigrocin-2 (Nigrocin-2LVa; Frogs, amphibians, animals)

    • Source

    • Rana nigromaculata (Black-spotted frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLLSKVLGVGKKVLCGVSGLC

    • Sequence Length

    • 21

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.11682065]Gram-positive bacterium:
        Target OrganismActivity
        Micrococcus luteus(MIC=2.5μg/ml);-
      • Gram-negative bacteria:
        Target OrganismActivity
        Shigella dysenteriaeMIC=10μg/ml
        Klebsiella pneumoniaeMIC=10μg/ml
        Pseudomonas aeruginosaMIC=100μg/ml
        Salmonella typhimuriumMIC=22.5μg/ml
        Proteus mirabilisMIC>200μg/ml
        Serratia marcescens(MIC>200μg/ml);-
      • Yeast:
        Target OrganismActivity
        Candida albicansMIC=150μg/ml

    • Hemolytic Activity

      • [Ref:11682065] It has 0.9% hemolytic activity at 100μg/ml against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys15 and Cys21)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys15 and Cys21.

    • Stereochemistry

    • L

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C90H164N24O24S2

    • Absent Amino Acids

    • ADEFHIMNPQRTWY

    • Common Amino Acids

    • GL

    • Mass

    • 2030.56

    • PI

    • 9.39

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +3

    • Boman Index

    • 24.57

    • Hydrophobicity

    • 1.214

    • Aliphatic Index

    • 148.1

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.25

    • Polar Residues

    • 9

DRAMP01445

DRAMP01445 chydropathy plot

Comments Information

    • Function

    • Thanks to its single linear amphipathic alpha-helix, may integrate into membrane phospholipids, leading to lysis of the membrane. Shows antibacterial activity against both Gram-positive and Gram-negative bacteria and against the fungus C. albicans. Has weak hemolytic activity.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

    • PTM

    • Contains one disulfide bond 15-21. (By similarity)

Literature Information

  • ·Literature 1

    • Title

    • Structural study of novel antimicrobial peptides, nigrocins, isolated from Rana nigromaculata.

    • Reference

    • FEBS Lett. 2001 Oct 19;507(1):95-100.

    • Author

    • Park S, Park SH, Ahn HC, Kim S, Kim SS, Lee BJ, Lee BJ.