General Information

    • DRAMP ID

    • DRAMP01553

    • Peptide Name

    • Caerin-1.4 (Frogs, amphibians, animals)

    • Source

    • Litoria caerulea (Green tree frog) (also Litoria gilleni) (Centralian tree frog)

    • Family

    • Belongs to the frog skin active peptide family (Caerin subfamily)

    • Gene

    • Not found

    • Sequence

    • GLLSSLSSVAKHVLPHVVPVIAEHL

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

    • SMILES

    • CC[C@@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)CN)C(C)C)C(C)C)C(C)C)C(C)C)C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CC(C)C)C(N)=O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria:
        Target OrganismActivity
        Bacillus cereusMIC=50 µg/ml
        Leuconostoc lactisMIC=12 µg/ml
        Listeria innocuaMIC=100 µg/ml
        Micrococcus luteusMIC=0.4 µg/ml
        Staphylococcus aureusMIC=100 µg/ml
        Staphylococcus epidermisMIC=25 µg/ml
        Streptococcus uberis (MIC=100 µg/ml);-
      • Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coliMIC=50 µg/ml
        Pasteurella multocida (MIC=25 µg/ml).Ref.2

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • PDB ID

    • Predicted Structure

    • There is no predicted structure for DRAMP01553.

Physicochemical Information

    • Formula

    • C120H200N32O32

    • Absent Amino Acids

    • CDFMNQRTWY

    • Common Amino Acids

    • LV

    • Mass

    • 2603.1

    • PI

    • 7.02

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • 14.34

    • Hydrophobicity

    • 0.972

    • Aliphatic Index

    • 159.6

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 5

DRAMP01553

Comments Information

    • Function

    • Antibacterial peptide, that adopts an alpha helical conformation which can disrupt bacterial membranes. Each caerin displays a different antimicrobial specificity.

    • Tissue specificity

    • Expressed by the skin parotoid and/or rostral glands.

    • Domain

    • Contains two amphipathic alpha helix regions separated by a region of less-defined helicity and greater flexibility (By similarity).

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance.

    • Reference

    • Peptides. 2004 Jun;25(6):1035-1054.

    • Author

    • Apponyi MA, Pukala TL, Brinkworth CS, Maselli VM, Bowie JH, Tyler MJ, Booker GW, Wallace JC, Carver JA, Separovic F, Doyle J, Llewellyn LE.
  • ·Literature 2

    • Title

    • Peptides from Australian frogs. The structures of the caerins from Litoria caerula.

    • Reference

    • J. Chem. Res. 1993; 138: 910-936.

    • Author

    • Stone DJM, Waugh RJ, Bowie JH, Wallace JC, Tyler MJ.