General Information

    • DRAMP ID

    • DRAMP01668

    • Peptide Name

    • Dermaseptin-1 (DS I; Dermaseptin-S1, DS1; Frogs, amphibians, animals)

    • Source

    • Phyllomedusa sauvagei (Sauvage's leaf frog)

    • Family

    • Belongs to the frog skin active peptide family (Dermaseptin subfamily)

    • Gene

    • Not found

    • Sequence

    • ALWKTMLKKLGTMALHAGKAALGAAADTISQGTQ

    • Sequence Length

    • 34

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiprotozoal

    • Target Organism

      • Fungi:
        Target OrganismActivity
        Microsporum canis (IP 1194)MIC=50 µg/ml
        Tricophyton rubrum (IP 1400-82)MIC=100 µg/ml
        Arthroderma simii (IP 1063-74)MIC=100 µg/ml
        Aspergillus fumigatus (IP 1025-70)MIC=100 µg/ml
        Aerornonas caviae IP (67-16) TMIC=50 µg/ml
        Cryptococcus neoformans (IP 960-67)MIC=15 µg/ml
        Cryptococcus neoformans (IP 962-67)MIC=15 µg/ml
        Candida albicans (IP 884-65) (MIC=60 µg/ml);-
      • Gram-negative bacterium:
        Target OrganismActivity
        Escherichia coli (IP 76-24) (MIC=5 µg/ml);-
      • Gram-positive bacterium:
        Target OrganismActivity
        Nocardia brasiliensis (IP 16-80)MIC=200 µg/ml

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • PDB ID

    • Predicted Structure

Physicochemical Information

    • Formula

    • C152H257N43O44S2

    • Absent Amino Acids

    • CEFNPRVY

    • Common Amino Acids

    • A

    • Mass

    • 3455.09

    • PI

    • 10

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 15

    • Net Charge

    • +4

    • Boman Index

    • -5.55

    • Hydrophobicity

    • 0.185

    • Aliphatic Index

    • 92.35

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 166.67

    • Polar Residues

    • 9

DRAMP01668

DRAMP01668 chydropathy plot

Comments Information

    • Function

    • Possesses a potent antimicrobial activity against bacteria, fungi and protozoa. Probably acts by disturbing membrane functions with its amphipathic structure. This peptide could also inhibit HIV infection at a concentration that is also toxic to T cells (J Virol 2005; 79

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Isolation, amino acid sequence, and synthesis of dermaseptin, a novel antimicrobial peptide of amphibian skin.

    • Reference

    • Biochemistry. 1991 Sep 10;30(36):8824-8830.

    • Author

    • Mor A, Nguyen VH, Delfour A, Migliore-Samour D, Nicolas P.
  • ·Literature 2

    • Title

    • Isolation and structure of novel defensive peptides from frog skin.

    • Reference

    • Eur J Biochem. 1994 Jan 15;219(1-2):145-154.

    • Author

    • Mor A, Nicolas P.