General Information

    • DRAMP ID

    • DRAMP03825

    • Peptide Name

    • CNBr-cleaved lactoferricin Subfragment 2

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KKLGAPSITCVRRAFA

    • Sequence Length

    • 16

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

    • SMILES

    • CC[C@@H](C)[C@H](NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@H](C)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)O)C(C)C)[C@@H](C)O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coli L361MIC=50 µM
        Pseudomonas fluorescensMIC>100 µM
        Salmonella SalfordMIC>100 µM
        Escherichia coli O:9 (MIC=60 µM);-
      • Gram-positive bacteria:
        Target OrganismActivity
        Bacillus cereusMIC>100 µM
        Listeria monocytogenesMIC=40 µM
        Staphylococcus aureusMIC>100 µM

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • PDB ID

    • Predicted Structure

    • There is no predicted structure for DRAMP03825.

Physicochemical Information

    • Formula

    • C76H132N24O19S

    • Absent Amino Acids

    • DEHMNQWY

    • Common Amino Acids

    • A

    • Mass

    • 1718.09

    • PI

    • 11.01

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • -22.4

    • Hydrophobicity

    • 0.181

    • Aliphatic Index

    • 85.63

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP03825

Comments Information

    • Function

    • Subfragment 2 had weak antibacterial activity against L. monocytogenes and both E. coli isolates, being effective in the range of 40 to 60 µM.

Literature Information

  • ·Literature 1

    • Title

    • Antibacterial activity in bovine lactoferrin-derived peptides.

    • Reference

    • Antimicrob Agents Chemother. 1997 Jan;41(1):54-59.

    • Author

    • Hoek KS, Milne JM, Grieve PA, Dionysius DA, Smith R.