• • DRAMP ID

  • DRAMP20933

• • Peptide Name

  • Stigmurin

• • Source

  • Tityus stigmurus

• • Family

  • Derived from scorpion venom peptide

• • Gene

  • Not found

• • Sequence

  • FFSLIPSLVGGLISAFK

• • Sequence Length

  • 17

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Transcript level

• • SMILES

  • CC[C@@H](C)[C@H](NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)CNC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](N)Cc1ccccc1)[C@H](C)CC)C(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(N)=O

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal, Antiparasitic, Antiproliferative

• • Target Organism

  • • [Ref.29670004] Gram-positive bacteria:

      Target Organism	Activity
      Staphylococcus aureus ATCC 29213	MIC=9.38 μM
      Staphylococcus epidermidis ATCC 122225 (MIC=9.38 μM);	-

    • yeasts:

      Target Organism	Activity
      Candida albicans ATCC 90028 (MIC=37.5 μM);	-

    • Stigmurin inhibit 90% of the epimastigote forms of Trypanosoma cruzi growth at 25 ?M;
    • HeLa cell line:

      Target Organism	Activity
      IC50=7.98 μM	-

• • Hemolytic Activity

  • • [Ref.29670004]Hemolysis 0% at 1.17 μM, hemolysis 1% at 2.34 μM, hemolysis 1% at 4.69 μM, hemolysis 0% at 9.38 μM, hemolysis 1% at 18.75 μM, hemolysis 1% at 37.50 μM, hemolysis 3% at 75.00 μM against human red blood cell

• • Cytotoxicity

  • • [Ref.29670004] The cell viability of HeLa cell lines is 56%, 66%, 66%, 81%, 78%, 66% and 30% at peptide concentrations of 2, 4, 8, 10, 15, 20 and 40 μM.
    • The cell viability of 3T3 cell lines is 81%, 63%, 51%, 29%, 27%, 19% and 15% at peptide concentra

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP20933_predicted_structure.pdb to download.

DRAMP20933

• • Function

  • Antibacterial activity against the Gram-positive bacteria. Antifungal activity against yeasts.Antiparasitic Activity against epimastigote forms of Trypanosoma cruzi. Antiproliferative Activity against HeLa cells.

• ·Literature 1

• • Title

  • Analogs of the Scorpion Venom Peptide Stigmurin: Structural Assessment, Toxicity, and Increased Antimicrobial Activity.

• • Pubmed ID

  • 29670004

• • Reference

  • Toxins (Basel). 2018 Apr 18;10(4). pii: E161.

• • Author

  • Parente AMS, Daniele-Silva A, Furtado AA, Melo MA, Lacerda AF, Queiroz M, Moreno C, Santos E, Rocha HAO, Barbosa EG, Carvalho E, Silva-Júnior AA, Silva MS, Fernandes-Pedrosa MF.