• • DRAMP ID

  • DRAMP29043

• • Peptide Name

  • Oncocin (variant of Oncopeltus antibacterial peptide 4)

• • Source

  • Synthetic construct (Oncocin is a variant of the 2 kDa Oncopeltus antibacterial peptide 4, which was originally isolated from oncopeltus fasciatus (milkweed bug))

• • Family

  • Not found

• • Gene

• • Sequence

  • VDKPPYLPRPRPPRRIYNR

• • Sequence Length

  • 19

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • SMILES

  • CC[C@@H](C)[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H](N)C(C)C)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(=N)N)C(N)=O

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram-

• • Target Organism

  • • [Ref.20565063] Gram-negative pathogens:

      Target Organism	Activity
      P.aeruginosa DSM3227	MIC = 4 μg/mL
      P. aeruginosa Walter	MIC = 8 μg/mL
      P. aeruginosa ATCC27853	MIC = 4 μg/mL
      P. aeruginosa PAO1	MIC = 2 μg/mL
      P. aeruginosa 60811001	MIC = 2 μg/mL
      P. aeruginosa 60909001	MIC = 2 μg/mL
      P. aeruginosa 61118003	MIC = 2 μg/mL
      P. aeruginosa 70108001	MIC = 4 μg/mL
      E. coli 70329001	MIC = 1 μg/mL
      E. coli 70430001	MIC = 1 μg/mL
      E. coli 70419002	MIC = 8 μg/mL
      E. coli 70502001	MIC = 1 μg/mL
      E. coli Neumann	MIC = 2 μg/mL
      E. coli 205024	MIC = 4 μg/mL
      E. coli 455/7	MIC = 0.5 μg/mL
      E. coli 8474	MIC = 0.5 μg/mL
      K. pneumoniae DSM681	MIC = 0.25 μg/mL
      K. pneumoniae 57 USA	MIC = 0.25 μg/mL
      K. pneumoniae 63	MIC = 8 μg/mL
      K. pneumoniae 8085	MIC = 0.5 μg/mL
      K. pneumoniae ATCC12657	0.5 μg/mL
      K. pneumoniae ATCC27799	MIC = 1 μg/mL
      A. baumannii ICB14067	MIC = 1 μg/mL
      A. baumannii ICB14072	MIC = 1 μg/mL
      A. baumannii ICB14073	MIC = 2 μg/mL
      A. baumannii ICB9250	MIC = 0.5 μg/mL
      A. baumannii M17	MIC = 1 μg/mL
      E. cloacae 34654	MIC = 0.25 μg/mL
      E. cloacae Ha088	MIC = 0.125 μg/mL
      E. cloacae Ha089	0.125 μg/mL
      E. cloacae Ha185	MIC = 0.5 μg/mL
      E. cloacae Ha89	MIC = 0.25 μg/mL
      P. vulgaris ICB9081	MIC = 4 μg/mL
      P. vulgaris ICB9095	MIC = 4 μg/mL
      Stenotrophomonas ICB308054	MIC = 32 μg/mL
      Stenotrophomonas ICB7569	MIC = 8 μg/mL
      Stenotrophomonas ICB7789	MIC = 32 μg/mL

• • Hemolytic Activity

  • • [Ref.20565063]None of the tested peptides showed any hemolytic activity against human erythrocytes in the studied peptide concentration range up to 600 μg/mL.

• • Cytotoxicity

  • • [Ref.20565063] Oncocin showed no toxic effects on HeLa- or neuronal-cell-like differentiated SH-SY5Y cells at a peptide concentration of 600 μg/mL, which was about 100-fold above their MIC-values against E.coli BL21 AI.

• • Binding Target

  • Bacterial ribosome

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • PDB ID

• Predicted Structure

• There is no predicted structure for DRAMP29043.

DRAMP29043

• • Comment

  • Oncocin inhibits bacterial protein translation by targeting the ribosome.

• ·Literature 1

• • Title

  • Oncocin (VDKPPYLPRPRPPRRIYNR-NH2): A Novel Antibacterial Peptide Optimized against Gram-Negative Human Pathogens

• • Pubmed ID

  • 20565063

• • Reference

  • J Med Chem. 2010 Jul 22;53(14):5240-7. doi: 10.1021/jm100378b.

• • Author

  • Daniel Knappe, Stefania Piantavigna, Anne Hansen, Adam Mechler, Annegret Binas, Oliver Nolte, Lisandra L Martin, Ralf Hoffmann

• ·Literature 2

• • Title

  • Differential stability of therapeutic peptides with different proteolytic cleavage sites in blood, plasma and serum

• • Pubmed ID

  • 28575099

• • Reference

  • PLoS One. 2017 Jun 2;12(6):e0178943. doi: 10.1371/journal.pone.0178943.

• • Author

  • Böttger R, Hoffmann R, Knappe D