• • DRAMP ID

  • DRAMP29044

• • Peptide Name

  • Cbf-K-16 (variant of BF-30)

• • Source

  • Synthetic construct (Cbf-K16 is a variant of BF-30, which was found in the venom of the snake bungarus fasciatus)

• • Family

  • Not found

• • Gene

• • Sequence

  • KFFRKLKKSVKKRAKKFFKKPRVIGVSIPF

• • Sequence Length

  • 30

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • SMILES

  • CC[C@@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](N)CCCCN)C(C)C)C(C)C)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)O)[C@H](C)CC)C(C)C

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.31704464] Gram-negative drug-resistant clinical isolates:

      Target Organism	Activity
      H. pylori SS1 (MIC = 16 μg/mL, MBC = 32 μg/mL); Gram -negative bacteria: H. pylori 26695	MIC = 32 μg/mL, MBC = 64 μg/mL
      H. pylori 11637	MIC = 16 μg/mL, MBC = 64 μg/mL

    • [Ref.27287456] Gram-positive drug-resistant clinical isolates:

      Target Organism	Activity
      MRSA strain no.1	MIC = 32 μg/mL
      MRSA strain no.2	MIC = 16 μg/mL
      S.aureus strain no.1	MIC = 16 μg/mL
      S.aureus strain no.2	MIC = 64 μg/mL
      S.aureus strain no.3	MIC = 8 μg/mL
      S.aureus strain no.4	MIC = 16 μg/mL
      S.aureus strain no.5	MIC = 4 μg/mL
      S.aureus strain no.6	MIC = 8 μg/mL
      S.aureus strain no.7	MIC = 32 μg/mL
      S.epidermidis strain no.1	MIC = 16 μg/mL
      S.epidermidis strain no.2 (MIC = 32 μg/mL);	-

    • Gram-positive bacteria:

      Target Organism	Activity
      S.aureus ATCC 25923 (MIC = 8 μg/mL);	-

    • Gram-negative drug-resistant clinical isolates:

      Target Organism	Activity
      E.coli strain no.1	MIC = 4 μg/mL
      E.coli strain no.2	MIC = 8 μg/mL
      E.coli strain no.3 (MIC = 8 μg/mL);	-

    • Gram-negative bacteria:

      Target Organism	Activity
      E.coli ATCC 25922	MIC = 4 μg/mL

• • Hemolytic Activity

  • • [Ref.27287456]Cbf-K-16 has low hemolytic activity

• • Cytotoxicity

  • • [Ref.27287456] Cbf-K-16 has low cytotoxicity.

• • Binding Target

  • Genomic DNA

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • PDB ID

• Predicted Structure

• There is no predicted structure for DRAMP29044.

DRAMP29044

• • Comment

  • Cbf-K16 possessed effective antimicrobial and anti-inflammatory activities and downregulated the expression of adhesion- and cytotoxin-associated genes of drug-resistant H. pylori SS1, making it a potential candidate for anti-infective therapy.

• ·Literature 1

• • Title

  • Antimicrobial activities of peptide Cbf-K 16 against drug-resistant Helicobacter pylori infection in vitro and in vivo.

• • Pubmed ID

  • 31704464

• • Reference

  • Microb Pathog. 2020 Jan;138:103847. doi: 10.1016/j.micpath.2019.103847. Epub 2019 Nov 5.

• • Author

  • Jiang M, Ma L, Huang Y, Wu H, Dou J, Zhou C

• ·Literature 2

• • Title

  • The antimicrobial activity of Cbf-K16 against MRSA was enhanced by β-lactamantibiotics through cell wall non-integrity.

• • Pubmed ID

  • 27287456

• • Reference

  • Arch Pharm Res. 2016 Jul;39(7):978-88. doi: 10.1007/s12272-016-0769-x. Epub 2016 Jun 10.

• • Author

  • Bo Li, Wei Kang, Hanhan Liu, Yanrong Wang, Changzhong Yu, Xinyi Zhu, Jie Dou, Haibo Cai, Changlin Zhou