General Information

    • DRAMP ID

    • DRAMP29047

    • Peptide Name

    • PAMP (Proadrenomedullin N-terminal 20 peptide)

    • Source

    • Homo sapiens et.al

    • Family

    • Belongs to the adrenomedullin family

    • Gene

    • ADM

    • Sequence

    • ARLDVASEFRKKWNKWALSR

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

    • SMILES

    • CC(C)C[C@H](NC(=O)[C@H](C)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](C)N)C(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(=N)N)C(N)=O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.32439180] Gram-negative bacteria:
        Target OrganismActivity
        E. coliMIC = 8 μM
        P. aeruginosaMIC = 32 μM
        S. typhimuriumMIC = 16 μM
      • Gram-positive bacteria:
        Target OrganismActivity
        B. subtilisMIC = 8 μM
        S. epidermidisMIC = 8 μM
        S. aureusMIC = 16 μM

    • Hemolytic Activity

      • [Ref.32439180]HC10 > 256 μM against sheep RBCs.Note: HC10 is the minimum peptide concentration that caused >10% hemolysis of sheep red blood cells.

    • Cytotoxicity

      • Not found

    • Binding Target

    • Bacterial DNA

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Stereochemistry

    • L

    • Structure

    • α-helical

    • Structure Description

    • Not found

    • Predicted Structure

    • There is no predicted structure for DRAMP29047.

Physicochemical Information

    • Formula

    • C112H177N35O28

    • Absent Amino Acids

    • CGHIMPQTY

    • Common Amino Acids

    • AKR

    • Mass

    • 2461.86

    • PI

    • 11

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +4

    • Boman Index

    • -6343

    • Hydrophobicity

    • -0.955

    • Aliphatic Index

    • 68.5

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 578.95

    • Polar Residues

    • 3

DRAMP29047

Comments Information

    • Introduction

    • Proadrenomedullin N-terminal 20 peptide (PAMP) is a biologically active molecule (20 amino acid residues) produced by post-translational enzymatic processing of the common precursor of adrenomedullin (AM), a 185-amino acid proadrenomedullin molecule. PMAP can bee found in various types. Function

Literature Information

  • ·Literature 1

    • Title

    • Proadrenomedullin N-terminal 20 peptide (PAMP) and its C-terminal 12-residue peptide, PAMP(9-20): Cell selectivity and antimicrobial mechanism

    • Reference

    • Biochem Biophys Res Commun. 2020 Jun 30;527(3):744-750. doi: 10.1016/j.bbrc.2020.04.063. Epub 2020 May 18.

    • Author

    • Ajish C, Yang S, Kumar SD, Shin SY.