General Information

    • DRAMP ID

    • DRAMP29060

    • Peptide Name

    • GHaR6R (variant of temporin-GHaR)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Sequence

    • FLQRIRGALGRLF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

    • SMILES

    • CC[C@@H](C)[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)Cc1ccccc1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.33291521] Gram-positive bacteria:
        Target OrganismActivity
        S. aureusMIC / MBC = 3.1μM / 12.5μM
        B. subtilisMIC / MBC = 25μM / 50μM
        S. mutansMIC / MBC = 12.5μM / 12.5μM
        MRSAMIC / MBC = 6.2μM / 12.5μM
        MRSA-1MIC /MBC = 12.5μM / 25μM
        MRSA-2MIC / MBC = 12.5μM / 25μM
        MRSA-3MIC / MBC = 6.2μM / 25μM
      • Gram-negative bacteria:
        Target OrganismActivity
        E. coliMIC / MBC = 12.5μM / 12.5μM
        D31MIC /MBC = 25μM / 50μM
        P. aeruginosaMIC / MBC > 50μM / 50μM
        PAO1MIC / MBC = 3.1μM / 25μM
      • Fungi:
        Target OrganismActivity
        C. albicansMIC / MBC = 50μM / 50μM

    • Hemolytic Activity

      • [Ref.33291521]HL10 = 150μM against human red blood cells (hRBCs)

    • Cytotoxicity

      • [Ref.33291521]GHaR6R induced a cell viability of 98%, 95% and 83% to human oral epithelial cells at 3.1μM, 6.2μM and 12.5μM.

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Stereochemistry

    • L

    • Structure

    • α-helical

    • Structure Description

    • Not found

    • PDB ID

    • Predicted Structure

    • There is no predicted structure for DRAMP29060.

Physicochemical Information

    • Formula

    • C72H119N23O15

    • Absent Amino Acids

    • CDEHKMNPSTVWY

    • Common Amino Acids

    • LR

    • Mass

    • 1546.88

    • PI

    • 12.3

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +3

    • Boman Index

    • -2097

    • Hydrophobicity

    • 0.423

    • Aliphatic Index

    • 127.69

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP29060

Comments Information

    • Comment

    • GHaR6R not only exerted antimicrobial activity by destroying cell membranes of planktonic bacteria, but also exhibited anti-biofilm activity. The low-toxicity peptides of GHaR6R is expected to become promising antimicrobial candidate to develop anti-caries agents.

Literature Information

  • ·Literature 1

    • Title

    • Temporin-Like Peptides Show Antimicrobial and Anti-Biofilm Activities against Streptococcus mutans with Reduced Hemolysis

    • Reference

    • Molecules. 2020 Dec 4;25(23):5724. doi: 10.3390/molecules25235724.

    • Author

    • Wei H, Xie Z, Tan X, Guo R, Song Y, Xie X, Wang R, Li L, Wang M, Zhang Y