• • DRAMP ID

  • DRAMP29061

• • Peptide Name

  • GHaR7R (variant of temporin-GHaR)

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

• • Sequence

  • FLQRIIRALGRLF

• • Sequence Length

  • 13

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • SMILES

  • CC[C@@H](C)[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)Cc1ccccc1)C(=O)N[C@H](C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)O)[C@H](C)CC

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.33291521] Gram-positive bacteria:

      Target Organism	Activity
      S. aureus	MIC / MBC = 3.1μM / 3.1μM
      B. subtilis	MIC / MBC > 50μM / 50μM
      S. mutans	MIC / MBC = 6.2μM / 12.5μM
      MRSA	MIC / MBC = 3.1μM / 6.2μM
      MRSA-1	MIC /MBC = 6.2μM / 25μM
      MRSA-2	MIC / MBC = 6.2μM / 50μM
      MRSA-3	MIC / MBC = 6.2μM / 25μM

    • Gram-negative bacteria:

      Target Organism	Activity
      E. coli	MIC / MBC = 3.1μM / 12.5μM
      D31	MIC /MBC = 12.5μM / 50μM
      P. aeruginosa	MIC / MBC > 50μM / 50μM
      PAO1	MIC / MBC > 50μM / 50μM

    • Fungi:

      Target Organism	Activity
      C. albicans	MIC / MBC = 25μM / 25μM

• • Hemolytic Activity

  • • [Ref.33291521]HL50 = 10μM against human red blood cells (hRBCs)

• • Cytotoxicity

  • • [Ref.33291521]GHaR6R induced a cell viability of 62%, 50% and 39% to human oral epithelial cells at 3.1μM, 6.2μM and 12.5μM.

• • Binding Target

  • Cell membrane

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

• • Stereochemistry

  • L

• • Structure

  • α-helical

• • Structure Description

  • Not found

• • PDB ID

• Predicted Structure

• There is no predicted structure for DRAMP29061.

DRAMP29061

• • Comment

  • GHaR6R not only exerted antimicrobial activity by destroying cell membranes of planktonic bacteria, but also exhibited anti-biofilm activity. However, the hemolysis and cytotoxicity to HOECs of GHaR7R is too strong to make it not suitable be a good antimicrobial candidate

• ·Literature 1

• • Title

  • Temporin-Like Peptides Show Antimicrobial and Anti-Biofilm Activities against Streptococcus mutans with Reduced Hemolysis

• • Pubmed ID

  • 33291521

• • Reference

  • Molecules. 2020 Dec 4;25(23):5724. doi: 10.3390/molecules25235724.

• • Author

  • Wei H, Xie Z, Tan X, Guo R, Song Y, Xie X, Wang R, Li L, Wang M, Zhang Y