General Information

    • DRAMP ID

    • DRAMP38994

    • Peptide Name

    • Longicin P4

    • Source

    • Haemaphysalis longicornis (Tick)

    • Family

    • Belongs to the invertebrate defensin family. Type 2 subfamily.

    • Gene

    • HlDfs

    • Sequence

    • IGRRGGYCAGIIKQTCTCYRK

    • Sequence Length

    • 21

    • Protein Existence

    • transcript level

    • SMILES

    • Not available

Activity Information

    • Biological Activity

    • Antimicrobial,Antibacterial,Anti-Gram-,Antiprotozoal

    • Target Organism

      • [Ref.17485458]protozoon: Babesia(Parasitemia remained stable at approximately 1% of 5.0μmol treatment)
      • Gram-negative bacteria:
        Target OrganismActivity
        E.coli(2μmol,CFU<10^3);P.aeruginosa(2μmol,CFU<10^3);S.aureus(2μmol,CFU<10^3);S.typhimurium10μmol,CFU<10^3
      • [Ref.22102016]protozoon: B. bigemina(50% inhibition at 50μM)

    • Hemolytic Activity

      • [Ref.17485458]Will not cause hemolysis at any concentration that exerted a parasiticidal effect.

    • Cytotoxicity

      • Not found

    • Binding Target

    • plasma membrane

Structure Information

    • Linear/Cyclic

    • Not found

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Not found

    • Stereochemistry

    • L

    • Structure

    • Structure Description

    • Predicted Structure

Physicochemical Information

    • Formula

    • C99H167N33O27S3

    • Absent Amino Acids

    • Asp,Glu,Phe,His,L

    • Common Amino Acids

    • Ala,Cys,G

    • Mass

    • 2347.79

    • PI

    • 9.75

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +5

    • Boman Index

    • 0

    • Hydrophobicity

    • -0.004

    • Aliphatic Index

    • 60.48

    • Half Life

      • Mammalian:20 hours
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3105

    • Absorbance 280nm

    • 1.32

    • Polar Residues

    • 8

DRAMP38994

Comments Information

Literature Information

  • ·Literature 1

    • Title

    • Babesial vector tick defensin against Babesia sp. parasites.

    • Reference

    • Infect Immun. 2007 May 7;75(7):3633–3640.

    • Author

    • Tsuji N, Battsetseg B, Boldbaatar D, Miyoshi T, Xuan X, Oliver JH Jr, Fujisaki K.
  • ·Literature 2

    • Title

    • Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis.

    • Reference

    • Trop Anim Health Prod. 2012 Feb;44(2):343-8.

    • Author

    • Galay RL, Maeda H, Aung KM, Umemiya-Shirafuji R, Xuan X, Igarashi I, Tsuji N, Tanaka T, Fujisaki K.