General Information

    • DRAMP ID

    • DRAMP39265

    • Peptide Name

    • CZS-4

    • Source

    • Cruziohyla calcarifer (Splendid leaf frog) (Agalychnis calcarifer)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Cruzioseptin subfamily.

    • Gene

    • Not found

    • Sequence

    • GFLDVIKHVGKAALSVVSHLINE

    • Sequence Length

    • 23

    • Protein Existence

    • protein level

    • SMILES

    • Not available

Activity Information

    • Biological Activity

    • Antimicrobial,Antibacterial,Anti-Gram+,Anti-Gram-,antifungal,Antiprotozoal

    • Target Organism

      • [Ref.39012112]protozoon: T. cruzi Trypomastigotes(IC50=1.55µM)；T. cruzi Amastigotes(IC50=30.65µM)；P. falciparum NF54(IC50=13.86µM)；P. falciparum TM90C2B(IC50=4.87µM)；L. mexicana(IC50=0.09µM)
      • Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coliIC50=13.09µM
      • Gram-positive bacteria:
        Target OrganismActivity
        Staphylococcus aureusIC50=26.18µM
      • Fungi:
        Target OrganismActivity
        Candida albicansMIC=52.36µM

    • Hemolytic Activity

      • [Ref.39012112]Induced hemolysis of 13% at 128 µM.

    • Cytotoxicity

      • [Ref.39012112]IC50=70.66µM targeting on LLC-mk2 cells;IC50=47.96µM targeting on Raw 264.7 cells

    • Binding Target

    • plasma membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Not found

    • Stereochemistry

    • L

    • Predicted Structure

    • There is no predicted structure for DRAMP39265.

Physicochemical Information

    • Formula

    • C112H184N30O31

    • Absent Amino Acids

    • Cys,Met,Pro,Gln,A

    • Common Amino Acids

    • Ala,Asp,G

    • Mass

    • 2446.84

    • PI

    • 6.93

    • Basic Residues

    • 4

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 12

    • Net Charge

    • 0

    • Boman Index

    • 0

    • Hydrophobicity

    • 0.007

    • Aliphatic Index

    • 143.91

    • Half Life

      • Mammalian:30 hours
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP39265

DRAMP39265 chydropathy plot

Comments Information

Literature Information

  • ·Literature 1

    • Title

    • Bioactivity of synthetic peptides from Ecuadorian frog skin secretions against Leishmania mexicana, Plasmodium falciparum, and Trypanosoma cruzi.

    • Reference

    • Microbiol Spectr. 2024 Aug 6;12(8):e0333923.

    • Author

    • Proaño-Bolaños C, Morán-Marcillo G, Espinosa de Los Monteros-Silva N, Bermúdez-Puga S, Salazar MA, Blasco-Zúñiga A, Cuesta S, Molina C, Espinosa F, Meneses L, Rojas-Silva P, Zapata Mena S, Sáenz FE, Rivera I M, Costales JA.