General Information

    • DRAMP ID

    • DRAMP00193

    • Peptide Name

    • Microcin B17 (MccB17; Bacteriocin)

    • Source

    • Escherichia coli (Gram-negative bacteria)

    • Family

    • Belongs to the class I microcin

    • Gene

    • mcbA

    • Sequence

    • VGIGGGGGGGGGGSCGGQGGGCGGCSNGCSGGNGGSGGSGSHI

    • Sequence Length

    • 43

    • Protein Existence

    • Protein level

    • SMILES

    • Not available

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C118H188N48O53S4

    • Absent Amino Acids

    • ADEFKLMPRTWY

    • Common Amino Acids

    • G

    • Mass

    • 3255.32

    • PI

    • 6.68

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 3

    • Net Charge

    • +1

    • Boman Index

    • -0.44

    • Hydrophobicity

    • -0.133

    • Aliphatic Index

    • 24.88

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 250

    • Absorbance 280nm

    • 5.95

    • Polar Residues

    • 38

DRAMP00193

Comments Information

    • Function

    • This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.

    • PTM

    • Maturation of thiazole and oxazole containing antibiotics involves the enzymic condensation of a Cys, Ser or Thr with the alpha-carbonyl of the preceding amino acid to form a thioether or ether bond, then dehydration to form a double bond with the alpha-amino nitrogen. Thiazoline or oxazoline rings are dehydrogenated to form thiazole or oxazole rings.

Literature Information

  • ·Literature 1

    • Title

    • The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by Escherichia coli DNA gyrase.

    • Reference

    • EMBO J. 1991 Feb;10(2):467-476.

    • Author

    • Viz¡n JL, Hern¡ndez-Chico C, del Castillo I, Moreno F.
  • ·Literature 2

    • Title

    • The DNA replication inhibitor microcin B17 is a forty-three-amino-acid protein containing sixty percent glycine.

    • Reference

    • Proteins. 1986 Nov;1(3):230-238.

    • Author

    • Davagnino J, Herrero M, Furlong D, Moreno F, Kolter R.