General Information

    • DRAMP ID

    • DRAMP00276

    • Peptide Name

    • Snakin-2 (StSN2; Cys-rich; Plant defensin)

    • Source

    • Solanum tuberosum (Potato)

    • Family

    • Not found

    • Gene

    • SN2

    • Sequence

    • YSYKKIDCGGACAARCRLSSRPRLCNRACGTCCARCNCVPPGTSGNTETCPCYASLTTHGNKRKCP

    • Sequence Length

    • 66

    • Protein Existence

    • Protein level

    • SMILES

    • Not available

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal

    • Target Organism

      • Gram-positive bacteria:
        Target OrganismActivity
        Clavibacter michiganensisEC50=4 µM
        Ralstonia solanacearumEC50=15 µM
      • Fungi:
        Target OrganismActivity
        pathogens Rhizobium melilotiEC50=8 µM
        Botrytis cinereaEC50=2 µM
        Fusarium solaniEC50=2 µM
        Fusarium culmorumEC50=2 µM
        Fusarium oxysporum f.sp. conglutinansEC50=10 µM
        Fusarium oxysporum f.sp.lycopersiciEC50=20 µM
        Plectosphaerella cucumerinaEC50=10 µM
        Colletotrichum graminicolaEC50=10 µM
        Colletotrichum lagenariumEC50=10 µM
        Bipolaris maydisEC50=20 µM
        Aspergillus flavusEC50=20 µM

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00276.

Physicochemical Information

    • Formula

    • C283H467N97O89S12

    • Absent Amino Acids

    • FMQW

    • Common Amino Acids

    • C

    • Mass

    • 7037.14

    • PI

    • 9.16

    • Basic Residues

    • 12

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +10

    • Boman Index

    • -150.65

    • Hydrophobicity

    • -0.499

    • Aliphatic Index

    • 37.12

    • Half Life

      • Mammalian:2.8 hour
      • Yeast:10 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5220

    • Absorbance 280nm

    • 80.31

    • Polar Residues

    • 36

DRAMP00276

Comments Information

    • Function

    • Has an antimicrobial activity. Causes a rapid aggregation of both Gram-positive and Gram-negative bacteria, but the antimicrobial activity is not correlated with the capacity to aggregate bacteria.

    • Tissue specificity

    • Expressed in tubers, stems, flowers, shoot apex and leaves, but not in roots or stolons.

    • Induction

    • Locally up-regulated by abscisic acid, wounding and infection with compatible fungus. Down-regulated by gibberellic acid or infection with virulent bacteria. No responses to ethylene or abiotic stresses.

    • PTM

    • Six disulfide bonds may be present.

Literature Information

  • ·Literature 1

    • Title

    • Snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection.

    • Reference

    • Plant Physiol. 2002 Mar;128(3):951-961.

    • Author

    • Marta Berrocal-Lobo, Ana Segura, Manuel Moreno, Gemma López, Francisco García-Olmedo, Antonio Molina