General Information

    • DRAMP ID

    • DRAMP00337

    • Peptide Name

    • ChaC8 (Chassatide C8; uncyclotides; Plant defensin)

    • Source

    • Chassalia chartacea

    • Family

    • Belongs to the Bracelet subfamily, Rubiaceae family

    • Gene

    • Not found

    • Sequence

    • AIPCGESCVWIPCISTVIGCSCSNKVCYR

    • Sequence Length

    • 29

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

    • SMILES

    • CC[C@@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N1CCC[C@H]1C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)O)C(C)C)[C@H](C)CC)[C@@H](C)O)[C@H](C)CC)NC(=O)CNC(=O)[C@H](CS)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](C)N)[C@H](C)CC)C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)C(C)C

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacterium:
        Target OrganismActivity
        Escherichia coli (MIC=6.6 µM);-
      • Gram-positive bacteria:
        Target OrganismActivity
        Staphylococcus aureusMIC>80 µM
        Staphylococcus epidermidisMIC>80 µM

    • Hemolytic Activity

      • [Ref.22467870] HD50 = 25.5 μM against human red blood cells (Type A). Kalata B1 and melittin were used as the positive control.

    • Cytotoxicity

      • [Ref.22467870] IC50 = 1.0 μM targeting on HeLa cells. Kalata B1 was used as the positive control.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • PDB ID

    • Predicted Structure

    • There is no predicted structure for DRAMP00337.

Physicochemical Information

    • Formula

    • C131H211N35O39S6

    • Absent Amino Acids

    • DFHLMQ

    • Common Amino Acids

    • C

    • Mass

    • 3092.69

    • PI

    • 7.81

    • Basic Residues

    • 2

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +1

    • Boman Index

    • -4.73

    • Hydrophobicity

    • 0.755

    • Aliphatic Index

    • 87.24

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 7365

    • Absorbance 280nm

    • 263.04

    • Polar Residues

    • 15

DRAMP00337

Comments Information

    • IC50=1.0 µM, HD50=25.5 µM.

    • Note

    • IC50 (concentration that gives a survival index of 50%) and HD50 (concentration that causes 50% lysis of red blood cells).

Literature Information

  • ·Literature 1

    • Title

    • Novel Cyclotides and Uncyclotides with Highly Shortened Precursors from Chassalia chartacea and Effects of Methionine Oxidation on Bioactivities.

    • Reference

    • J Biol Chem. 2012 May 18;287(21):17598-17607.

    • Author

    • Nguyen GK, Lim WH, Nguyen PQ, Tam JP.