General Information

    • DRAMP ID

    • DRAMP03865

    • Peptide Name

    • Sub6 (Bac2A variant through six amino acids substitution)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RWWKIWVIRWWR

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

    • SMILES

    • CC[C@@H](C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(=N)N)C(N)=O)[C@H](C)CC)C(C)C

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria:
        Target OrganismActivity
        Pseudomonas aeruginosaMIC=31 µg/ml
        Escherichia coliMIC=4 µg/ml
        Salmonella typhimurium (MIC=16 µg/ml);-
      • Gram-positive bacteria:
        Target OrganismActivity
        Staphylococcus aureusMIC=8 µg/ml
        Staphylococcus epidermidisMIC=1 µg/ml
        Enterococcus faecalisMIC=8 µg/ml
      • Yeast:
        Target OrganismActivity
        Candida albicansMIC=31 µg/ml

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • PDB ID

    • Predicted Structure

    • There is no predicted structure for DRAMP03865.

Physicochemical Information

    • Formula

    • C96H131N27O13

    • Absent Amino Acids

    • ACDEFGHLMNPQSTY

    • Common Amino Acids

    • W

    • Mass

    • 1871.27

    • PI

    • 12.3

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +4

    • Boman Index

    • -24.78

    • Hydrophobicity

    • -0.725

    • Aliphatic Index

    • 89.17

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 27500

    • Absorbance 280nm

    • 2500

    • Polar Residues

    • 0

DRAMP03865

Comments Information

    • Function

    • Optimized 12-mer peptides shows broad spectrum antibacterial activity against Gram-positive and Gram-negative bacteria. Also has antifungal activity against C. albicans.

    • Chemical modification

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • High-throughput generation of small antibacterial peptides with improved activity.

    • Reference

    • Nat Biotechnol. 2005 Aug;23(8):1008-1012.

    • Author

    • Hilpert K, Volkmer-Engert R, Walter T, Hancock RE.