General Information

    • DRAMP ID

    • DRAMP28989

    • Peptide Name

    • peptide 5 (Derived from Mag2)

    • Sequence

    • GIKKFLKSⓍKKFⓍKAFK

    • Sequence Length

    • 17

    • Original Sequence

    • GIGKFLHSAKKFGKAFVGEIMNS

    • Source

    • Synthetic construct

    • SMILES

    • CCC(C)[C@H](NC(=O)[C@@H2][NH3+])C(=O)N[C@H](CCCC[NH3+])C(=O)N[C@H](CCCC[NH3+])C(=O)N[C@H](Cc1ccccc1)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCC[NH3+])C(=O)N[C@H](CO)C(=O)N[C@]1(C)CCCC=CCCC[C@@](C)(C(=O)N[C@H](CCCC[NH3+])C(=O)N[C@H](C)C(=O)N[C@H](Cc2ccccc2)C(=O)N[C@@H](C=O)CCCC[NH3+])NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](CCCC[NH3+])NC(=O)[C@H](CCCC[NH3+])NC1=O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Comments

    • Function: Antibacterial activity against Gram-positive and Gram-negative bacteria

    • Target Organism

      • [Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 12.5 μM).
      • Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 6.25 μM), multiple-drug resistant P.aeruginosa (MIC = 3.125 μM)

    • Hemolytic Activity

      • [Ref.33466998] It has hemolysis against human red blood cells at 1.56 μM

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Special Amino Acid and Stapling Position

    • ①The Ⓧ (position: 9 and 13) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.

    • Stereochemistry

    • L

    • Secondary Structure

    • α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM

    • Structure Description

    • [Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.
  • There is no predicted structure for DRAMP28989.

Literature Information

  • Literature 1

    • Title

    • Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence

    • Reference

    • Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.

    • Author

    • Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu