DRAMP_ID Sequence Hiden_Sequence Original_Sequence Sequence_Length Name Uniprot_Entry Family Source Activity Protein_Existence Secondary_Structure Structure_Description PDB_ID Comments Target_Organism Hemolytic_Activity Linear/Cyclic N_terminal_Modification C_terminal_Modification Special_Amino_Acid_and_Stapling_Position Stereochemistry Cytotoxicity Pubmed_ID Reference Author Title Specific_Type Nucleotide_Sequence Full_Sequence lfcMLE padj Workflow SMILES DRAMP21468 KFFⓀKLKⓀAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 2 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 16 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL) [Ref.32216308] It has 1.9% hemolysis against human red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 4 and 8) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (4) and Ⓚ (8) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C(NC(=O)C(NC(=O)C1NC(=O)C(CCCC[NH3+])NC(=O)C(CC(C)C)NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc2ccccc2)Cc2ccccc2)CCCC[NH2+]CC=CC[NH2+]CCCC1)C)C(C)C DRAMP21469 KFFⓀKLKKAVⓀKGFKKFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 3 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 8 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 16 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 1.1% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 4 and 11) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (4) and Ⓚ (11) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC1C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH3+])CCCC[NH3+])Cc2ccccc2)CCCC[NH3+])CCCC[NH2+]CC=CC[NH2+]CCCC1)Cc1ccccc1)Cc1ccccc1)C([NH3+])CCCC[NH3+] DRAMP21470 KFFKⓀLKKAVKⓀGFKKFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 4 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 1.3% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 5 and 12) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (5) and Ⓚ (12) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC1C(=O)NC(CC(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH3+])CCCC[NH3+])Cc2ccccc2)CCCC[NH2+]CC=CC[NH2+]CCCC1)CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)C([NH3+])CCCC[NH3+] DRAMP21471 KFFKKLⓀKAVⓀKGFKKFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 5 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 4 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL) [Ref.32216308] It has 5.4% hemolysis against red blood cells at peptide concentration of 320 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 7 and 11) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (7) and Ⓚ (11) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH3+])CCCC[NH3+])Cc2ccccc2)CCCC[NH3+])CCCC[NH2+]CC=CC[NH2+]CCCC1)CC(C)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)C([NH3+])CCCC[NH3+] DRAMP21472 KFFKKLKⓀAVKⓀGFKKFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 6 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 2.9% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 8 and 12) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (8) and Ⓚ (12) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH3+])CCCC[NH3+])Cc2ccccc2)CCCC[NH2+]CC=CC[NH2+]CCCC1)CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)C([NH3+])CCCC[NH3+] DRAMP21473 KFFKKLKⓀAVKKGFⓀKFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 7 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 16 μg/mL), Listeria monocytogenes (MIC99.9= 8 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 16 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 2.6% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 8 and 15) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (8) and Ⓚ (15) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NCC(=O)NC(Cc2ccccc2)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH3+])CCCC[NH2+]CC=CC[NH2+]CCCC1)CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)C([NH3+])CCCC[NH3+] DRAMP21474 KFFKKLKKAVⓀKGFⓀKFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 8 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 2.7% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 11 and 15) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (11) and Ⓚ (15) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC1C(=O)NC(CCCC[NH3+])C(=O)NCC(=O)NC(Cc2ccccc2)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH3+])CCCC[NH2+]CC=CC[NH2+]CCCC1)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])C)C(C)C DRAMP21475 KFFKKLKKAVKⓀGFKKFAⓀV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 9 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 16 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL) [Ref.32216308] It has 5.4% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 12 and 19) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (19) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC1C(=O)NCC(=O)NC(Cc2ccccc2)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(Cc2ccccc2)C(=O)NC(C)C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH2+]CC=CC[NH2+]CCCC1)CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])C)C(C)C DRAMP21476 KFFKKLKKAVKⓀGFKⓀFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 10 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①41% α-helical content in 30 mM SDS. ②Random coils in PBS ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Stapled peptides 10 and 12 had a high content of α-helix of about 41 and 45%, respectively, respectively, which could partly explain their strong antibacterial activity and proteolytic resistance. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 2.8% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a (E)-but-2-enyl space employing the N-alkylation reactionr. L [Ref.32216308] The cell survial of HEK 293T cell line induced by peptide 10 is 101.8%, 100.7%, 98.9%, 89.1% and 83.9%. 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC1C(=O)NCC(=O)NC(Cc2ccccc2)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH2+]CC=CC[NH2+]CCCC1)CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])C)C(C)C DRAMP21477 KFFKKLKKAVKKGFⓀKFAⓀV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 11 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 4 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL) [Ref.32216308] It has 0.5% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 15 and 19) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (15) and Ⓚ (19) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC1C(=O)NC(CCCC[NH3+])C(=O)NC(Cc2ccccc2)C(=O)NC(C)C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH2+]CC=CC[NH2+]CCCC1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])C)C(C)C DRAMP21478 KFFKKLKKAVKⓀGFKⓀFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 12 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①45% α-helical content in 30 mM SDS. ②Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Stapled peptides 10 and 12 had a high content of α-helix of about 41 and 45%, respectively, respectively, which could partly explain their strong antibacterial activity and proteolytic resistance. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 2 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 4 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 8 μg/mL) [Ref.32216308] It has 8.7% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a 1,2-bismethylenebenzene spacer employing the N-alkylation. L [Ref.32216308] The cell survial of HEK 293T cell line induced by peptide 12 is 102.9%, 101.8%, 101.5%, 66.4% and 62.8%. 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC1C(=O)NCC(=O)NC(Cc2ccccc2)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH2+]Cc2c(cccc2)C[NH2+]CCCC1)CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])C)C(C)C DRAMP21479 KFFKKLKKAVKⓀGFKⓀFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 13 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 6.1% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a 1,3-bismethylenebenzene spacer employing the N-alkylation. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC1C(=O)NCC(=O)NC(Cc2ccccc2)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH2+]Cc2cc(ccc2)C[NH2+]CCCC1)CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])C)C(C)C DRAMP21480 KFFKKLKKAVKⓀGFKⓀFAKV KFFKKLKKAVKKGFKKFAKV KFFKKLKKAVKKGFKKFAKV 20 peptide 14 (derived from OH-CM6) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Random coils in PBS. ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL) [Ref.32216308] It has 6.8% hemolysis against red blood cells at peptide concentration of 320 μg/mL Cyclic (Stapled) Free Amidation ①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a 1,4-bismethylenebenzene spacer employing the N-alkylation. L No cytotoxicity information found in the reference 32216308 J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8. Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides Stapled AMP O=C(NC(C(=O)NC1C(=O)NCC(=O)NC(Cc2ccccc2)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C(C)C)CCCC[NH3+])C)Cc2ccccc2)CCCC[NH2+]Cc2ccc(cc2)C[NH2+]CCCC1)CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCCC[NH3+])Cc1ccccc1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])C)C(C)C DRAMP21482 KKKKKKAAFⓍAWAⓍFAA KKKKKKAAFXAWAXFAA KKKKKKAAFAAWAAFAA 17 S-6K-F17 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- ①Random coils with only a small amount of helical structure in aqueous buffer. ②α-helix in SDS detergent micelles. ①Similarly, the stapled peptide, S-6K-F17 is predominantly random coil in aqueous buffer with only a small amount of helical structure despite the presence of the staple - a feature likely due to the large stretch of non-helical Lys residues that flank the stapled portion of the sequence. ②As expected, in detergent micelles S-6K-F17 adopts a helical structure, paralleling the unstapled peptide. Not found Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded. [Ref.29275987] Gram-negative bacteria: E. coli (MIC= 1.0 μM) [Ref.29275987] MHC = 3.8 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 29275987 Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21. Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C)C)Cc2ccccc2)(C)CCCC=CCCC1)Cc1ccccc1)C)C)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C([NH3+])CCCC[NH3+] DRAMP21483 KKKKKKAGFⓍAWAⓍFGA KKKKKKAGFXAWAXFGA KKKKKKAAFAAWAAFAA 17 S-6K-F17-2G No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- Lack of significant structure Substitutions with polar, known 'helix-breaker' Gly residues led to losses in helical character until finally the peptide containing 3 Gly and 1 Asn (S-6K-F17-3GN) shows a severe loss in helical structure Not found Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded. [Ref.29275987] Gram-negative bacteria: E. coli (MIC= 1.6 μM) [Ref.29275987] MHC = 15 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 29275987 Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21. Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC1(C)C(=O)NC(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C=O)C)Cc2ccccc2)(C)CCCC=CCCC1)Cc1ccccc1)C)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C([NH3+])CCCC[NH3+] DRAMP21484 KKKKKKAGFⓍAWGⓍFGA KKKKKKAGFXAWGXFGA KKKKKKAAFAAWAAFAA 17 S-6K-F17-3G No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- Lack of significant structure Substitutions with polar, known 'helix-breaker' Gly residues led to losses in helical character until finally the peptide containing 3 Gly and 1 Asn (S-6K-F17-3GN) shows a severe loss in helical structure Not found Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded. [Ref.29275987] Gram-negative bacteria: E. coli (MIC= 4.2 μM) [Ref.29275987] MHC = 128 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 29275987 Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21. Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC1(C)C(=O)NC(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NCC(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C=O)C)Cc2ccccc2)(C)CCCC=CCCC1)Cc1ccccc1)C)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C([NH3+])CCCC[NH3+] DRAMP21485 KKKKKKNGFⓍAWGⓍFGA KKKKKKNGFXAWGXFGA KKKKKKAAFAAWAAFAA 17 S-6K-F17-3GN No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- Lack of significant structure Substitutions with polar, known 'helix-breaker' Gly residues led to losses in helical character until finally the peptide containing 3 Gly and 1 Asn (S-6K-F17-3GN) shows a severe loss in helical structure Not found Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded. [Ref.29275987] Gram-negative bacteria: E. coli (MIC= 4.2 μM) [Ref.29275987] MHC = 587 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 29275987 Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21. Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC1(C)C(=O)NC(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NCC(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C=O)C)Cc2ccccc2)(C)CCCC=CCCC1)Cc1ccccc1)CC(=O)N)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C([NH3+])CCCC[NH3+] DRAMP21486 VNWKKⓍLGKⓍIKVVK VNWKKXLGKXIKVVK VNWKKILGKIIKVVK 15 LL-IIIs-1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal ①20% α-helical content in water. ②55% α-helical content in 50% TFE. ③55% α-helical content in 8mM SDS. It seems that the staple in the central part of the LL-IIIs-1 analog that crosslink the bend of the α-helix on its concave site around the Gly8 residue resulted in slight helix destabilization. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.7 μM), Bacillus subtilis (MIC = 0.8 μM), Staphylococcus aureus (MIC = 12.5 μM);##Gram-negative bacteria: E.coli (MIC = 4.4 μM), Pseudomonas aeruginosa (MIC = 78.7 μM);##Fungi: Candida albicans (MIC = 100 μM). [Ref.22526241] LC50 = 31.3 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 6 and 10) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 18. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C(C)C)C(C)C)CCCC[NH3+])C(CC)C)(C)CCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])Cc1c2c([nH]c1)cccc2)CC(=O)N)C([NH3+])C(C)C DRAMP21487 VNWKKILGKⓍIKVⓍK VNWKKILGKXIKVXK VNWKKILGKIIKVVK 15 LL-IIIs-2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal ①31% α-helical content in water. ②51% α-helical content in 50% TFE. ③58% α-helical content in 8mM SDS. For the LL-IIIs-2 analog, the helical content increment is slightly higher (8 %). Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.9 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 20.3 μM);##Gram-negative bacteria: E.coli (MIC = 8.8 μM), Pseudomonas aeruginosa (MIC = 86.7 μM);##Fungi: Candida albicans (MIC = 100 μM). [Ref.22526241] LC50 = 69 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (10) and Ⓧ (14)are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 19. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC1(C)C(=O)NC(C(CC)C)C(=O)NC(CCCC[NH3+])C(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)CCCC[NH3+])CC(C)C)C(CC)C)CCCC[NH3+])CCCC[NH3+])Cc1c2c([nH]c1)cccc2)CC(=O)N)C([NH3+])C(C)C DRAMP21488 VⓍWKKⓍLGKIIKVVK VXWKKXLGKIIKVVK VNWKKILGKIIKVVK 15 LL-IIIs-3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal ①27% α-helical content in water. ②52% α-helical content in 50% TFE. ③62% α-helical content in 8mM SDS. The CD spectra of the singly stapled peptides of the i, i + 4 type acquired in water show a slight increase (by 5 %) of helical content in the case of MEP-Ns-1, MEP-Ns-2 and LL-IIIs-3 compared to their unstapled precursors. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.4 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 21.7 μM);##Gram-negative bacteria: E.coli (MIC = 1.2 μM), Pseudomonas aeruginosa (MIC = 76.3 μM);##Fungi: Candida albicans (MIC = 93.3 μM). [Ref.22526241] LC50 = 30 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 20. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C(C)C)C(C)C)CCCC[NH3+])C(CC)C)C(CC)C)CCCC[NH3+])CC(C)C)(C)CCCC=CCCC1)C([NH3+])C(C)C DRAMP21489 VNⓍKKIⓍGKⓍIKVⓍK VNXKKIXGKXIKVXK VNWKKILGKIIKVVK 15 LL-IIIs-4 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①45% α-helical content in water. ②48% α-helical content in 50% TFE. ③63% α-helical content in 8mM SDS. On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 % Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 2 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 80 μM);##Gram-negative bacteria: E.coli (MIC = 20 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 > 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 3, 7, 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (3) and Ⓧ (7), Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 21. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC1(C)C(=O)NC(C(CC)C)C(=O)NC(CCCC[NH3+])C(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)CCCC[NH3+])C)C(CC)C)CCCC[NH3+])CCCC[NH3+])C)CC(=O)N)C([NH3+])C(C)C DRAMP21490 VNⓏKKILGKⓍIKVVK VNZKKILGKXIKVVK VNWKKILGKIIKVVK 15 LL-IIIs-5 cis No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①22% α-helical content in water. ②54% α-helical content in 50% TFE. ③69% α-helical content in 8mM SDS. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.9 μM), Bacillus subtilis (MIC = 0.9 μM), Staphylococcus aureus (MIC = 45 μM);##Gram-negative bacteria: E.coli (MIC = 3.5 μM), Pseudomonas aeruginosa (MIC = 80 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 = 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓩ (position: 3) indicates 2-(7'-octenyl) alanine in the R configuration. ②The Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (3) and Ⓧ (10) are cross-linked by hydrocarbon stapling. L, cis No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 22. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(CC)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C(C)C)C(C)C)CCCC[NH3+])C(CC)C)(C)CCCC=CCCCCCC1)CC(=O)N)C([NH3+])C(C)C DRAMP21491 VNⓏKKILGKⓍIKVVK VNZKKILGKXIKVVK VNWKKILGKIIKVVK 15 LL-IIIs-5 trans No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①26% α-helical content in water. ②54% α-helical content in 50% TFE. ③62% α-helical content in 8mM SDS. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.1 μM), Bacillus subtilis (MIC = 1.3 μM), Staphylococcus aureus (MIC = 65 μM);##Gram-negative bacteria: E.coli (MIC = 5.5 μM), Pseudomonas aeruginosa (MIC = 80 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 > 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓩ (position: 3) indicates 2-(7'-octenyl) alanine in the R configuration. ②The Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (3) and Ⓧ (10) are cross-linked by hydrocarbon stapling. L, trans No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 23. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(CC)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C(C)C)C(C)C)CCCC[NH3+])C(CC)C)(C)CCCC=CCCCCCC1)CC(=O)N)C([NH3+])C(C)C DRAMP21492 VNⓍKKIⓍPKⓍIKVⓍK VNXKKIXPKXIKVXK VNWKKILGKIIKVVK 15 LL-IIIs-6a No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①36% α-helical content in water. ②42% α-helical content in 50% TFE. ③36% α-helical content in 8mM SDS. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 93 μM);##Gram-negative bacteria: E.coli (MIC = 7.8 μM), Pseudomonas aeruginosa (MIC = 93 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 > 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 3, 7, 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (3) and Ⓧ (7), Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling respectively. L, cis(around the S₅-Pro8 peptide bond) No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 24. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(CC)C)C(=O)NC(C(=O)N2C(C(=O)NC(C(=O)NC3(C)C(=O)NC(C(CC)C)C(=O)NC(CCCC[NH3+])C(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC3)CCCC[NH3+])CCC2)(C)CCCC=CCCC1)CC(=O)N)C([NH3+])C(C)C DRAMP21493 VNⓍKKIⓍPKⓍIKVⓍK VNXKKIXPKXIKVXK VNWKKILGKIIKVVK 15 LL-IIIs-6b No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①33% α-helical content in water. ②38% α-helical content in 50% TFE. ③35% α-helical content in 8mM SDS. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.9 μM), Bacillus subtilis (MIC = 0.8 μM), Staphylococcus aureus (MIC = 50 μM);##Gram-negative bacteria: E.coli (MIC = 7.8 μM), Pseudomonas aeruginosa (MIC = 63 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 = 82μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 3, 7, 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (3) and Ⓧ (7), Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling respectively. L, trans(around the S₅-Pro8 peptide bond) No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 25. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP DRAMP21495 GFLSILKKVLPKⓍJAHⓍK GFLSILKKVLPKXJAHXK GFLSILKKVLPKVMAHMK 18 MEP-Ns-1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal ①19% α-helical content in water.②66% α-helical content in 50% TFE. ③75% α-helical content in 8mM SDS. The CD spectra of the singly stapled peptides of the i, i + 4 type acquired in water show a slight increase (by 5 %) of helical content in the case of MEP-Ns-1, MEP-Ns-2 and LL-IIIs-3 compared to their unstapled precursors. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.8 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 10.8 μM);##Gram-negative bacteria: E.coli (MIC = 2.5 μM), Pseudomonas aeruginosa (MIC = 77 μM);##Fungi: Candida albicans (MIC = 30 μM). [Ref.22526241] LC50 = 18.1 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The J (position: 14) in sequence indicates norleucine. ②The Ⓧ (position: 13 and 17) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 28. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)N1C(C(=O)NC(C(=O)NC2(C)C(=O)NC(CCCC)C(=O)NC(C)C(=O)NC(Cc3nc[nH]c3)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC2)CCCC[NH3+])CCC1)CC(C)C)C(C)C)CCCC[NH3+])CCCC[NH3+])CC(C)C)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C[NH3+])Cc1ccccc1)CC(C)C)CO)C(CC)C DRAMP21496 GFLSⓍLKKⓍLPKVJAHJK GFLSXLKKXLPKVJAHJK GFLSILKKVLPKVMAHMK 18 MEP-Ns-2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①16% α-helical content in water.②61% α-helical content in 50% TFE. ③62% α-helical content in 8mM SDS. The CD spectra of the singly stapled peptides of the i, i + 4 type acquired in water show a slight increase (by 5 %) of helical content in the case of MEP-Ns-1, MEP-Ns-2 and LL-IIIs-3 compared to their unstapled precursors. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 37 μM);##Gram-negative bacteria: E.coli (MIC = 7.8 μM), Pseudomonas aeruginosa (MIC ≥ 100 μM);##Fungi: Candida albicans (MIC ≥ 100 μM). [Ref.22526241] LC50 = 14.7 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The J (position: 14 and 17) in sequence indicates norleucine. ②The Ⓧ (position: 5 and 9) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 29. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C)Cc1nc[nH]c1)C)CCCC)C(NC(=O)C(NC(=O)C1N(C(=O)C(NC(=O)C2(C)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C[NH3+])Cc3ccccc3)CC(C)C)CO)(C)CCCC=CCCC2)CC(C)C)CCC1)CCCC[NH3+])C(C)C DRAMP21497 GFLSⓍLKKⓍLPKⓍJAHⓍK GFLSXLKKXLPKXJAHXK GFLSILKKVLPKVMAHMK 18 MEP-Ns-3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①34% α-helical content in water.②56% α-helical content in 50% TFE. ③48% α-helical content in 8mM SDS. On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 % Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.3 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC ≥ 100 μM);##Gram-negative bacteria: E.coli (MIC = 46.7 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 = 13.9 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The J (position: 14) in sequence indicates norleucine. ②Ⓧ (position: 5, 9, 13 and 17) are 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9), Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 30. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC1(C)C(=O)NC(CC(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)N2C(C(=O)NC(C(=O)NC3(C)C(=O)NC(CCCC)C(=O)NC(C)C(=O)NC(Cc4nc[nH]c4)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC3)CCCC[NH3+])CCC2)CC(C)C)(C)CCCC=CCCC1)CO)C(NC(=O)C(NC(=O)C[NH3+])Cc1ccccc1)CC(C)C DRAMP21498 GFⓏSILKKVⓍPKVJAHJK GFZSILKKVXPKVJAHJK GFLSILKKVLPKVMAHMK 18 MEP-Ns-4 cis No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Unknown No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.3 μM), Staphylococcus aureus (MIC = 37 μM);##Gram-negative bacteria: E.coli (MIC = 8.1 μM), Pseudomonas aeruginosa (MIC ≥ 100 μM);##Fungi: Candida albicans (MIC ≥ 100 μM). [Ref.22526241] LC50 = 11 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The J (position: 14 and 17) in sequence indicates norleucine. ②Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (position: 3) is 2-(7'-pctenyl) lalnine in the R configuration. ④Ⓧ (10) and Ⓩ (3) are cross-linked by hydrocarbon stapling. L, cis No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 31. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])CCCC)Cc1nc[nH]c1)C)C)C(NC(=O)C(NC(=O)C1N(C(=O)C2(C)NC(=O)C(C(C)C)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(CC(C)C)NC(=O)C(C(CC)C)NC(=O)C(CO)NC(=O)C(NC(=O)C(NC(=O)C[NH3+])Cc3ccccc3)(C)CCCCCCC=CCCC2)CCC1)CCCC[NH3+])C(C)C DRAMP21499 GFⓏSILKKVⓍPKVJAHJK GFZSILKKVXPKVJAHJK GFLSILKKVLPKVMAHMK 18 MEP-Ns-4 trans No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Unknown No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 2 μM), Bacillus subtilis (MIC = 1.7 μM), Staphylococcus aureus (MIC = 63 μM);##Gram-negative bacteria: E.coli (MIC = 16.3 μM), Pseudomonas aeruginosa (MIC ≥ 100 μM);##Fungi: Candida albicans (MIC ≥ 100 μM). [Ref.22526241] LC50 = 20 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The J (position: 14 and 17) in sequence indicates norleucine. ②Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (position: 3) is 2-(7'-pctenyl) lalnine in the R configuration. ④Ⓧ (10) and Ⓩ (3) are cross-linked by hydrocarbon stapling. L, trans No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 32. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])CCCC)Cc1nc[nH]c1)C)CCCC)C(NC(=O)C(NC(=O)C1N(C(=O)C2(C)NC(=O)C(C(C)C)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(CC(C)C)NC(=O)C(C(CC)C)NC(=O)C(CO)NC(=O)C(NC(=O)C(NC(=O)C[NH3+])Cc3ccccc3)(C)CCCCCCC=CCCC2)CCC1)CCCC[NH3+])C(C)C DRAMP21500 GFLSⓍLKKⓍLGKⓍJAHⓍK GFLSXLKKXLGKXJAHXK GFLSILKKVLPKVMAHMK 18 MEP-Ns-5 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①52% α-helical content in water.②67% α-helical content in 50% TFE. ③59% α-helical content in 8mM SDS. On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 % Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 57 μM);##Gram-negative bacteria: E.coli (MIC = 5.6 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 = 29 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The J (position: 14) in sequence indicates norleucine. ②Ⓧ (position: 5, 9, 13 and 17) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9), Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 33. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC1(C)C(=O)NC(CC(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC2(C)C(=O)NC(CCCC)C(=O)NC(C)C(=O)NC(Cc3nc[nH]c3)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC2)CCCC[NH3+])CC(C)C)(C)CCCC=CCCC1)CO)C(NC(=O)C(NC(=O)C[NH3+])Cc1ccccc1)CC(C)C DRAMP21501 GFLSⓍLKKⓍLAKⓍJAHⓍK GFLSXLKKXLAKXJAHXK GFLSILKKVLPKVMAHMK 18 MEP-Ns-6 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①56% α-helical content in water.②64% α-helical content in 50% TFE. ③56% α-helical content in 8mM SDS. On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 % Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM. [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 43 μM);##Gram-negative bacteria: E.coli (MIC = 5.6 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM). [Ref.22526241] LC50 = 39.5 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay. Cyclic (Stapled) Free Amidation ①The J (position: 14) in sequence indicates norleucine. ②Ⓧ (position: 5, 9, 13 and 17) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9), Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 22526241 Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 34. Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera Stapled AMP O=C(NC(C(=O)NC1(C)C(=O)NC(CC(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC2(C)C(=O)NC(CCCC)C(=O)NC(C)C(=O)NC(Cc3nc[nH]c3)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC2)CCCC[NH3+])C)CC(C)C)(C)CCCC=CCCC1)CO)C(NC(=O)C(NC(=O)C[NH3+])Cc1ccccc1)CC(C)C DRAMP21502 IDWKKLLⓀAAKⒼIL IDWKKLLKAAKGIL IDWKKLLDAAKQIL 14 C-MP1-1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①Slight α-helical structure in aqueous solution. ②Increased α-helical conformation in 30 mM SDS and 50% TFE compared with MPI ①By CD, we observed that C-MPI-2 and MPI did not display any structural preferences in aqueous solution, whereas C-MPI-1 adopoted a slight α-helical structure. ②C-MPI-1 also had higher α-helicity than MPI in membrane mimicking environments, including 30 mM sodium dodecyl sulfate (SDS) and 50% trifluoroethyl alcohol (TFE). Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28833783] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 64 μM), Bacillus subtilis ATCC 23857 (MIC = 8 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 64 μM), Pseudomonas aeruginosa ATCC 27853 (No antimicrobial activity) [Ref.28833783] It has 0%, 0%, 2.1%, 9.5%, 22.7%, 25.4% and 34.7% against human red blood cells at peptide concentrations of 0, 5, 10, 25, 50, 75 and 150 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓚ (position: 8) is lysine with the methyltrityl side chain. ②The Ⓖ (position: 12) is propargylglycine. ③Ⓚ (8) and Ⓖ (12) are cross-linked by hydrocarbon stapling by 1,3-diploar azide-alkyne cyclization. L No cytotoxicity information found in the reference 28833783 J Pept Sci. 2017 Nov;23(11):824-832. doi: 10.1002/psc.3031. Epub 2017 Aug 23. Beijun Liu, Wei Zhang, Sanhu Gou, Haifeng Huang, Jia Yao, Zhibin Yang, Hui Liu, Chao Zhong, Beiyin Liu, Jingman Ni, Rui Wang Intramolecular cyclization of the antimicrobial peptide Polybia-MPI with triazole stapling: influence on stability and bioactivity Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CC(C)C)C(CC)C)CC=N)CCCC[NH3+])C)C)CCCC[NH3+])CC(C)C)CC(C)C)CCCC[NH3+])CCCC[NH3+])Cc1c2c([nH]c1)cccc2)CC(=O)[O-])C([NH3+])C(CC)C.[H+H0] DRAMP21503 IⓀWKKLLⒼAAKQIL IKWKKLLGAAKQIL IDWKKLLDAAKQIL 14 C-MP1-2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- No structural preference in aqueous solution. By CD, we observed that C-MPI-2 and MPI did not display any structural preferences in aqueous solution, whereas C-MPI-1 adopoted a slight α-helical structure. Not found Function: Antibacterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria is not so evident. [Ref.28833783] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 256 μM), Bacillus subtilis ATCC 23857 (MIC = 128 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 128 μM), Pseudomonas aeruginosa ATCC 27853 (No antimicrobial activity) [Ref.28833783] No hemolytic information found. Cyclic (Stapled) Acetylation Amidation ①The Ⓚ (position: 2) is lysine with the methyltrityl side chain. ②The Ⓖ (position: 8) is propargylglycine. ③Ⓚ (2) and Ⓖ (8) are cross-linked by hydrocarbon stapling by 1,3-diploar azide-alkyne cyclization. L No cytotoxicity information found in the reference 28833783 J Pept Sci. 2017 Nov;23(11):824-832. doi: 10.1002/psc.3031. Epub 2017 Aug 23. Beijun Liu, Wei Zhang, Sanhu Gou, Haifeng Huang, Jia Yao, Zhibin Yang, Hui Liu, Chao Zhong, Beiyin Liu, Jingman Ni, Rui Wang Intramolecular cyclization of the antimicrobial peptide Polybia-MPI with triazole stapling: influence on stability and bioactivity Stapled AMP O=C(NC1C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CC(C)C)C(CC)C)CCC(=O)N)CCCC[NH3+])C)C)CC[NH2+]CCCC1)C([NH3+])C(CC)C.[NH4+].[H+H0] DRAMP21504 ⓍIGKⓍLHSAKKFGKAFVGEIJNS XIGKXLHSAKKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)0 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 15% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 59.46 μg/mL), Bacillus cereus ATCC 14579 (MIC = 10.49 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 25.00 μg/mL) [Ref.31427820] It has 5.6% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 1 and 5) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(C(CC)C)NC(=O)C([NH3+])(C)CCCC=CCCC1)CC(C)C)Cc1[nH]cnc1)CO)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21505 GⓍGKFⓍHSAKKFGKAFVGEIJNS GXGKFXHSAKKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 12% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 100.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 14.87 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.72 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 12.50 μg/mL) [Ref.31427820] It has 4.6% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(NC(=O)C[NH3+])(C)CCCC=CCCC1)Cc1nc[nH]c1)CO)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21506 GIⓍKFLⓍSAKKFGKAFVGEIJNS GIXKFLXSAKKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 21% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 7.43 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 10.51 μg/mL) [Ref.31427820] It has 18.3% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 3 and 7) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (3) and Ⓧ (7) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(CC(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C[NH3+])C(CC)C)(C)CCCC=CCCC1)CO)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21507 GIGⓍFLHⓍAKKFGKAFVGEIJNS GIGXFLHXAKKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 18% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.84 μg/mL), Bacillus cereus ATCC 14579 (MIC = 10.51 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 7.43 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 25.00 μg/mL) [Ref.31427820] It has 42.1% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 4 and 8) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (4) and Ⓧ (8) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(Cc2[nH]cnc2)NC(=O)C(CC(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)(C)CCCC=CCCC1)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21508 GIGKⓍLHSⓍKKFGKAFVGEIJNS GIGKXLHSXKKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)4 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 24% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 6.25 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 10.51 μg/mL) [Ref.31427820] It has 18.3% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 5 and 9) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (5) and Ⓧ (9) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(CO)NC(=O)C(Cc2[nH]cnc2)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])(C)CCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21509 GIGKFⓍHSAⓍKFGKAFVGEIJNS GIGKFXHSAXKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)5 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 14% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 17.68 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 14.87 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 50.00 μg/mL) [Ref.31427820] It has 13.2% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 6 and 10) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(C)NC(=O)C(CO)NC(=O)C(Cc2[nH]cnc2)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)(C)CCCC=CCCC1)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21510 GIGKFLⓍSAKⓍFGKAFVGEIJNS GIGKFLXSAKXFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)6 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 18% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 7.43 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 29.73 μg/mL) [Ref.31427820] It has 7.6% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 7 and 11) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (7) and Ⓧ (11) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)C(C)NC(=O)C(CO)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)(C)CCCC=CCCC1)Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21511 GIGKFLHⓍAKKⓍGKAFVGEIJNS GIGKFLHXAKKXGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)7 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 26% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.25 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.25 μg/mL) [Ref.31427820] It has 62.4% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 8 and 12) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (8) and Ⓧ (12) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(C)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)(C)CCCC=CCCC1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21512 GIGKFLHSⓍKKFⓍKAFVGEIJNS GIGKFLHSXKKFXKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)8 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 49% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.44 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.72 μg/mL) [Ref.31427820] It has 74.6% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 9 and 13) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)(C)CCCC=CCCC1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21513 GIGKFLHSAⓍKFGⓍAFVGEIJNS GIGKFLHSAXKFGXAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)9 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 28% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 17.68 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 59.46 μg/mL) [Ref.31427820] It has 60.0% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 10 and 14) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)CNC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)C)(C)CCCC=CCCC1)C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21514 GIGKFLHSAKⓍFGKⓍFVGEIJNS GIGKFLHSAKXFGKXFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)10 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 15% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.84 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 14.87 μg/mL) [Ref.31427820] It has 23.9% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 11 and 15) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (11) and Ⓧ (15) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(Cc2ccccc2)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2nc[nH]c2)CO)C)CCCC[NH3+])(C)CCCC=CCCC1)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21515 GIGKFLHSAKKⓍGKAⓍVGEIJNS GIGKFLHSAKKXGKAXVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)11 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 16% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 8.84 μg/mL) [Ref.31427820] It has 17.8% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 12 and 16) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (12) and Ⓧ (16) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C1(C)NC(=O)C(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)C)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21516 GIGKFLHSAKKFⓍKAFⓍGEIJNS GIGKFLHSAKKFXKAFXGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)12 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 51% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.43 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 4.42 μg/mL) [Ref.31427820] It has 76.6% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 13 and 17) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C1(C)NC(=O)C(Cc2ccccc2)NC(=O)C(C)NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)C)CCCC[NH3+])CCCC[NH3+])Cc2ccccc2)(C)CCCC=CCCC1)CCC(=O)[O-])C(CC)C DRAMP21517 GIGKFLHSAKKFGⓍAFVⓍEIJNS GIGKFLHSAKKFGXAFVXEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)13 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 23% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 5.26 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 7.43 μg/mL) [Ref.31427820] It has 60.0% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 14 and 18) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (14) and Ⓧ (18) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(C(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(C)NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)C)CCCC[NH3+])CCCC[NH3+])Cc2ccccc2)(C)CCCC=CCCC1)CCC(=O)[O-])C(CC)C DRAMP21518 GIGKFLHSAKKFGKⓍFVGⓍIJNS GIGKFLHSAKKFGKXFVGXIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)14 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 19% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 2.63 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.86 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.13 μg/mL) [Ref.31427820] It has 92.4% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 15 and 19) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (15) and Ⓧ (19) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C1(C)NC(=O)CNC(=O)C(C(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)C)CCCC[NH3+])CCCC[NH3+])Cc2ccccc2)CCCC[NH3+])(C)CCCC=CCCC1)C(CC)C DRAMP21519 GIGKFLHSAKKFGKAⓍVGEⓍJNS GIGKFLHSAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)15 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 15% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 10.51 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 7.43 μg/mL) [Ref.31427820] It has 11.7% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21520 GIGKFLHSAKKFGKAFⓍGEIⓍNS GIGKFLHSAKKFGKAFXGEIXNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)16 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 11% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 42.04 μg/mL), Bacillus cereus ATCC 14579 (MIC = 7.44 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.72 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 8.84 μg/mL) [Ref.31427820] It has 8.1% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 17 and 21) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (17) and Ⓧ (21) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(CC)C)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)(C)CCCC=CCCC1)Cc1ccccc1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21521 GIGKFLHSAKKFGKAFVⓍEIJⓍS GIGKFLHSAKKFGKAFVXEIJXS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)17 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 30% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 1.86 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL) [Ref.31427820] It has 56.3% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 18 and 22) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (18) and Ⓧ (22) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CCC(=O)[O-])C(=O)NC(C(CC)C)C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CO)(C)CCCC=CCCC1)C(C)C)Cc1ccccc1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21522 GIGKFLHSAKKFGKAFVGⓍIJNⓍ GIGKFLHSAKKFGKAFVGXIJNX GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(I+4)18 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 19% α-helical content in 25-50 μM potassium phosphate solution. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 1.56 μg/mL), Bacillus cereus ATCC 14579 (MIC = 2.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.21 μg/mL) [Ref.31427820] It has 101.0% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 19 and 23) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (19) and Ⓧ (23) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC1(C)C(=O)NC(C(CC)C)C(=O)NC(CCCC)C(=O)NC(CC(=O)N)C(=O)NC(C=O)(C)CCCC=CCCC1)C(C)C)Cc1ccccc1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1nc[nH]c1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21523 KIGKFLHSAKKFGKAⓍVGEⓍJNS KIGKFLHSAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(G1K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 21.02 μg/mL), Bacillus cereus ATCC 14579 (MIC = 10.49 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.41 μg/mL) [Ref.31427820] It has 4.4% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C([NH3+])CCCC[NH3+])C(CC)C DRAMP21524 GKGKFLHSAKKFGKAⓍVGEⓍJNS GKGKFLHSAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(I2K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 35.36 μg/mL), Bacillus cereus ATCC 14579 (MIC = 25.00 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 4.75 μg/mL) [Ref.31427820] It has 2.2% hemolysis against human red blood cells at 25 μg/mL.. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CC(C)C)Cc1[nH]cnc1)CO DRAMP21525 GIKKFLHSAKKFGKAⓍVGEⓍJNS GIKKFLHSAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(G3K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 10.49 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.34 μg/mL) [Ref.31427820] It has 3.7% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21526 GIGKKLHSAKKFGKAⓍVGEⓍJNS GIGKKLHSAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(F5K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.69 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 17.68 μg/mL) [Ref.31427820] It has 1.5% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)CCCC[NH3+])CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21527 GIGKFKHSAKKFGKAⓍVGEⓍJNS GIGKFKHSAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(L6K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 17.68 μg/mL) [Ref.31427820] It has 1.7% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1nc[nH]c1)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21528 GIGKFLKSAKKFGKAⓍVGEⓍJNS GIGKFLKSAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(H7K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.20 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.69 μg/mL) [Ref.31427820] It has 3.1% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)CCCC[NH3+])CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21529 GIGKFLHKAKKFGKAⓍVGEⓍJNS GIGKFLHKAKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(S8K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.20 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.38 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.65 μg/mL) [Ref.31427820] It has 3.3% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CCCC[NH3+])Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21530 GIGKFLHSKKKFGKAⓍVGEⓍJNS GIGKFLHSKKKFGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(A9K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.38 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 15.81 μg/mL) [Ref.31427820] It has 1.7% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21531 GIGKFLHSAKKKGKAⓍVGEⓍJNS GIGKFLHSAKKKGKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(F12K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 14.03 μg/mL) [Ref.31427820] It has 1.9% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21532 GIGKFLHSAKKFKKAⓍVGEⓍJNS GIGKFLHSAKKFKKAXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(G13K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.10 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 12.82 μg/mL) [Ref.31427820] It has 1.8% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21533 GIGKFLHSAKKFGKKⓍVGEⓍJNS GIGKFLHSAKKFGKKXVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(A15K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 31.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 9.89 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.10 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 4.97 μg/mL) [Ref.31427820] It has 6.5% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21534 GIGKFLHSAKKFGKAⓍKGEⓍJNS GIGKFLHSAKKFGKAXKGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(V17K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.91 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 19.84 μg/mL) [Ref.31427820] It has 2.1% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21535 GIGKFLHSAKKFGKAⓍVKEⓍJNS GIGKFLHSAKKFGKAXVKEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(G18K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.80 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.38 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.23 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.57 μg/mL) [Ref.31427820] It has 8.3% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21536 GIGKFLHSAKKFGKAⓍVGKⓍJNS GIGKFLHSAKKFGKAXVGKXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(E19K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.23 μg/mL) [Ref.31427820] It has 6.0% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21537 GIGKFLHSAKKFGKAⓍVGEⓍKNS GIGKFLHSAKKFGKAXVGEXKNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(B21K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 29.73 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.23 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 8.97 μg/mL) [Ref.31427820] It has 3.2% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC[NH3+])(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21538 GIGKFLHSAKKFGKAⓍVGEⓍJKS GIGKFLHSAKKFGKAXVGEXJKS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(N22K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 21.02 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.38 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.57 μg/mL) [Ref.31427820] It has 11.9% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CCCC[NH3+])CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21539 GIGKFLHSAKKFGKAⓍVGEⓍJNK GIGKFLHSAKKFGKAXVGEXJNK GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)15(S23K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 42.04 μg/mL), Bacillus cereus ATCC 14579 (MIC = 7.39 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.23 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.57 μg/mL) [Ref.31427820] It has 4.9% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])CC(=O)N)CCCC)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21540 GⓍGSFⓍKKKAHVGKHⓍGKAⓍLTHYL GXGSFXKKKAHVGKHXGKAXLTHYL GWGSFFKKAAHVGKHVGKAALTHYL 25 Pleu(i+4)1,15(A9K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 3.13 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.13 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 1.56 μg/mL) [Ref.31427820] It has 17.1% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CC(C)C)Cc1ccc(O)cc1)Cc1nc[nH]c1)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C2(C)NC(=O)C(Cc3ccccc3)NC(=O)C(CO)NC(=O)CNC(=O)C(NC(=O)C[NH3+])(C)CCCC=CCCC2)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C)Cc2[nH]cnc2)C(C)C)CCCC[NH3+])Cc2nc[nH]c2)(C)CCCC=CCCC1)CC(C)C)C(O)C DRAMP21541 GⓍRKRⓍRKFRNKIKEKKKKIGQKⓍQGLⓍPKLA GXRKRXRKFRNKIKEKKKKIGQKXQGLXPKLA GLRKRLRKFRNKIKEKLKKIGQKIQGFVPKLAPRTDY 32 CAP(i+4)1,23(L17K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.5 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.13 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 1.56 μg/mL) [Ref.31427820] It has 4.0% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2, 6, 24 and 28) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (24) and X (28) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CCC(=O)N)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(C(=O)N2C(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C)CC(C)C)CCCC[NH3+])CCC2)(C)CCCC=CCCC1)CCCC[NH3+])CCC(=O)N)C(CC)C)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])CCC(=O)[O-])CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(CCCNC(=[NH2+])N)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCNC(=[NH2+])N)NC(=O)C(NC(=O)C[NH3+])(C)CCCC=CCCC1)CCCNC(=[NH2+])N)CCCC[NH3+])Cc1ccccc1)CCCNC(=[NH2+])N)CC(=O)N)CCCC[NH3+])C(CC)C DRAMP21542 GⓍGKFⓍHSKKKFGKAⓍVGEⓍBNS GXGKFXHSKKKFGKAXVGEXBNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+4)1,15(A9K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 6.25 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.12 μg/mL) [Ref.31427820] It has 1.9% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The B (position: 21) in sequence is norlercine. ③Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)C)(C)CCCC=CCCC1)C)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C(NC(=O)C(NC(=O)C1(C)NC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(NC(=O)C[NH3+])(C)CCCC=CCCC1)Cc1nc[nH]c1)CO DRAMP21543 GⓍFSKⓍKGKKIKNLⓍISGⓍKG GXFSKXKGKKIKNLXISGXKG GIFSKLAGKKIKNLLISGLK 21 Esc(i+4)1,14(A7K) No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 1.56 μg/mL) [Ref.31427820] It has 2.5% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2, 6, 15 and 19) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (15) and Ⓧ (19) are cross-linked by hydrocarbon stapling respectively. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(C(CC)C)C(=O)NC(CO)C(=O)NCC(=O)NC(C(=O)NC(C(=O)NCC=O)CCCC[NH3+])(C)CCCC=CCCC1)CC(C)C)CC(=O)N)CCCC[NH3+])C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)C(CO)NC(=O)C(Cc2ccccc2)NC(=O)C(NC(=O)C[NH3+])(C)CCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])CCCC[NH3+])C(CC)C DRAMP21544 GⓏGKFLHSⓍKKFGKAFVGEIJNS GZGKFLHSXKKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 10.51 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.72 μg/mL) [Ref.31427820] It has 49.7% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 9) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 2) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (9) and Ⓩ (2) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(CO)NC(=O)C(Cc2[nH]cnc2)NC(=O)C(CC(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(NC(=O)C[NH3+])(C)CCCCCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21545 GIⓏKFLHSAⓍKFGKAFVGEIJNS GIZKFLHSAXKFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 8.84 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 21.02 μg/mL) [Ref.31427820] It has 66.1% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 10) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 3) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (10) and Ⓩ (3) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(C)NC(=O)C(CO)NC(=O)C(Cc2[nH]cnc2)NC(=O)C(CC(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C[NH3+])C(CC)C)(C)CCCCCCC=CCCC1)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21546 GIGⓏFLHSAKⓍFGKAFVGEIJNS GIGZFLHSAKXFGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 14.87 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 10.51 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 21.02 μg/mL) [Ref.31427820] It has 52.6% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 11) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 4) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (11) and Ⓩ (4) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)C(C)NC(=O)C(CO)NC(=O)C(Cc2nc[nH]c2)NC(=O)C(CC(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)(C)CCCCCCC=CCCC1)Cc1ccccc1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21547 GIGKⓏLHSAKKⓍGKAFVGEIJNS GIGKZLHSAKKXGKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)4 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 21.02 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.25 μg/mL) [Ref.31427820] It has 42.5% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 12) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 5) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (12) and Ⓩ (5) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(C)NC(=O)C(CO)NC(=O)C(Cc2[nH]cnc2)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])(C)CCCCCCC=CCCC1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21548 GIGKFⓏHSAKKFⓍKAFVGEIJNS GIGKFZHSAKKFXKAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)5 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.84 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.13 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.13 μg/mL) [Ref.31427820] It has 82.9% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 13) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 6) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (13) and Ⓩ (6) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(C)NC(=O)C(CO)NC(=O)C(Cc2[nH]cnc2)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)(C)CCCCCCC=CCCC1)CCCC[NH3+])C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21549 GIGKFLⓏSAKKFGⓍAFVGEIJNS GIGKFLZSAKKFGXAFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)6 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.25 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 14.87 μg/mL) [Ref.31427820] It has 45.3% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 14) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 7) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (14) and Ⓩ (7) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)CNC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(C)NC(=O)C(CO)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)(C)CCCCCCC=CCCC1)C)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21550 GIGKFLHⓏAKKFGKⓍFVGEIJNS GIGKFLHZAKKFGKXFVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)7 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 3.72 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL) [Ref.31427820] It has 95.3% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 15) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 8) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (15) and Ⓩ (8) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(C)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)(C)CCCCCCC=CCCC1)Cc1ccccc1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21551 GIGKFLHSⓏKKFGKAⓍVGEIJNS GIGKFLHSZKKFGKAXVGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)8 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.25 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.72 μg/mL) [Ref.31427820] It has 85.2% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 16) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 9) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (16) and Ⓩ (9) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C1(C)NC(=O)C(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)(C)CCCCCCC=CCCC1)C(C)C)CCC(=O)[O-])C(CC)C DRAMP21552 GIGKFLHSAⓏKFGKAFⓍGEIJNS GIGKFLHSAZKFGKAFXGEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)9 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 3.72 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.72 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL) [Ref.31427820] It has 94.6% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 17) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 10) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (17) and Ⓩ (10) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)CNC(=O)C1(C)NC(=O)C(Cc2ccccc2)NC(=O)C(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(Cc2ccccc2)NC(=O)C(CCCC[NH3+])NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)C)(C)CCCCCCC=CCCC1)CCC(=O)[O-])C(CC)C DRAMP21553 GIGKFLHSAKⓏFGKAFVⓍEIJNS GIGKFLHSAKZFGKAFVXEIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)10 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 4.42 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 7.43 μg/mL) [Ref.31427820] It has 43.8% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 18) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 11) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (18) and Ⓩ (11) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(C(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(Cc2ccccc2)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2nc[nH]c2)CO)C)CCCC[NH3+])(C)CCCCCCC=CCCC1)CCC(=O)[O-])C(CC)C DRAMP21554 GIGKFLHSAKKⓏGKAFVGⓍIJNS GIGKFLHSAKKZGKAFVGXIJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)11 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 2.63 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.21 μg/mL) [Ref.31427820] It has 84.7% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 19) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 12) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (19) and Ⓩ (12) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)C(NC(=O)C1(C)NC(=O)CNC(=O)C(C(C)C)NC(=O)C(Cc2ccccc2)NC(=O)C(C)NC(=O)C(CCCC[NH3+])NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C[NH3+])C(CC)C)CCCC[NH3+])Cc2ccccc2)CC(C)C)Cc2[nH]cnc2)CO)C)CCCC[NH3+])CCCC[NH3+])(C)CCCCCCC=CCCC1)C(CC)C DRAMP21555 GIGKFLHSAKKFⓏKAFVGEⓍJNS GIGKFLHSAKKFZKAFVGEXJNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)12 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.44 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.13 μg/mL) [Ref.31427820] It has 75.4% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 20) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 13) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (20) and Ⓩ (13) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(Cc2ccccc2)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)CCCC)(C)CCCC=CCCCCCC1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21556 GIGKFLHSAKKFGⓏAFVGEIⓍNS GIGKFLHSAKKFGZAFVGEIXNS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)13 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.44 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.25 μg/mL) [Ref.31427820] It has 63.5% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 21) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 14) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③Ⓧ (21) and Ⓩ (14) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC1(C)C(=O)NC(C)C(=O)NC(Cc2ccccc2)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(CC)C)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CO)CC(=O)N)(C)CCCC=CCCCCCC1)Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21557 GIGKFLHSAKKFGKⓏFVGEIJⓍS GIGKFLHSAKKFGKZFVGEIJXS GIGKFLHSAKKFGKAFVGEIMNS 23 Mag(i+7)14 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in potassium phosphate buffer. No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 1.56 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.86 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL) [Ref.31427820] It has 68.1% hemolysis against human red blood cells at 25 μg/mL. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 22) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 15) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (22) and Ⓩ (15) are cross-linked by hydrocarbon stapling. L No cytotoxicity information found in the reference 31427820 Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19. Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice Stapled AMP O=C(NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC1(C)C(=O)NC(Cc2ccccc2)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CCC(=O)[O-])C(=O)NC(C(CC)C)C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CO)(C)CCCC=CCCCCCC1)CCCC[NH3+])Cc1ccccc1)CCCC[NH3+])CCCC[NH3+])C)CO)Cc1[nH]cnc1)CC(C)C)Cc1ccccc1)CCCC[NH3+])C(NC(=O)C[NH3+])C(CC)C DRAMP21558 KⓍWKJⓍK KXWKJXK / 7 KKK No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). ①[Ref.30361948] All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. ②[Ref.28547390] Peptide NLE and LYS maintained a compatible helicity to LEU. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM), Staphylococcus epidermidis ATCC 12228 (MIC= 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM).##[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 6.3 μg/mL), Staphylococcus aureus (MIC = 6.3 μg/mL), Staphylococcus epidermidis (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 12.5 μg/mL), Shigella dysenteriae (MIC = 12.5 μg/mL), Salmonella typhimurium (MIC = 50 μg/mL), Klebsiella pneumoniae (MIC = 18.8 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL). [Ref.30361948] It has 1.5% hemolysis against human red blood cells at 25 μM and 3.0% hemolysis at 50 μM.##[Ref.28547390] 3.3% hemolysis against human red blood cells at 25 μM and 7.9% hemolysis t 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948##28547390 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.##Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim. ##Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides.##Mono-substitution effects on antimicrobial activity of stapled heptapeptides. Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21559 RⓍWKJⓍK RXWKJXK / 7 RKK No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 6.3 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM), Staphylococcus epidermidis ATCC 12228 (MIC= 12.5 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 25 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 12.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM) [Ref.30361948] It has 1.5% hemolysis against human red blood cells at 25 μM and 4.3% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCNC(=[NH2+])N DRAMP21560 KⓍWRJⓍK KXWRJXK / 7 KRK No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM), Staphylococcus epidermidis ATCC 12228 (MIC= 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysenteriae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 75 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM) [Ref.30361948] It has <1% hemolysis against human red blood cells at 25 μM and 2.0% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCNC(=[NH2+])N)C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21561 KⓍWKJⓍR KXWKJXR / 7 KKR No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 9.4 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM), Staphylococcus epidermidis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 12.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM) [Ref.30361948] It has 1.4% hemolysis against human red blood cells at 25 μM and % hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCNC(=[NH2+])N)(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21562 RⓍWRJⓍK RXWRJXK / 7 RRK No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM), Staphylococcus epidermidis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysenteriae ATCC 9752 (MIC = 37.5 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 37.5 μM) [Ref.30361948] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCNC(=[NH2+])N)C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCNC(=[NH2+])N DRAMP21563 RⓍWKJⓍR RXWKJXR / 7 RKR No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 6.3 μM), Staphyolococcus aureus ATCC 6538p (MIC = 3.2 μM), Staphylococcus epidermidis ATCC 12228 (MIC = 12.5 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 6.3 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 25 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM) [Ref.30361948] It has 8.2% hemolysis against human red blood cells at 25 μM and 17.0% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCNC(=[NH2+])N)(C)CCCC=CCCC1)C([NH3+])CCCNC(=[NH2+])N DRAMP21564 KⓍWRJⓍR KXWRJXR / 7 KRR No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 6.3 μM), Staphyolococcus aureus ATCC 6538p (MIC = 3.2 μM), Staphylococcus epidermidis ATCC 12228 (MIC = 12.5 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 37.5 μM) [Ref.30361948] It has 1.8% hemolysis against human red blood cells at 25 μM and 5.6% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCNC(=[NH2+])N)C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCNC(=[NH2+])N)(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21565 RⓍWRJⓍR RXWRJXR / 7 RRR No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate solution (pH 6.5). All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM), Staphylococcus epidermidis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysenteriae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM) [Ref.30361948] It has <1% hemolysis against human red blood cells at 25 μM and 3.2% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 30361948 Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCNC(=[NH2+])N)C(=O)NC(CCCC)C(=O)NC(C(=O)NC(C=O)CCCNC(=[NH2+])N)(C)CCCC=CCCC1)C([NH3+])CCCNC(=[NH2+])N DRAMP21566 IDWKKⓍLDAⓍKQIL IDWKKXLDAXKQIL IDWKKLLDAAKQIL 14 MP1S No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+ 96.1% α-helical content in a 25 mM potassium phosphate buffer solution at 20℃ ①Helical contents of all stapled analogues were increased by more than a three-fold compared to their unmodified counterpart, MP1. ②MP1S appears to be the most helical among this panel of peptides, showing 96% helicity, which is 3.7-fold higher than that of MP1. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC = 1.6 μg/mL), Staphylococcus aureus (MIC = 2.4 μg/mL), Staphylococcus epidermidis (MIC > 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 100 μg/mL), Shigella dysenteriae (MIC > 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC > 100 μg/mL), Pseudomonas aeruginosa (MIC > 100 μg/mL) [Ref.29075946] It has 6.7% hemolysis against human red blood cells at 12.5 μM and 12.3% hemolysis at 25 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 29075946 Arch Pharm Res. 2017 Dec;40(12):1414-1419. doi: 10.1007/s12272-017-0963-5. Epub 2017 Oct 26. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity and stability of stapled helices of polybia-MP1 Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CC(C)C)C(=O)NC(CC(=O)[O-])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CC(C)C)C(CC)C)CCC(=O)N)CCCC[NH3+])(C)CCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])Cc1c2c([nH]c1)cccc2)CC(=O)[O-])C([NH3+])C(CC)C DRAMP21567 IDWKKⓍLNAⓍKQIL IDWKKXLNAXKQIL IDWKKLLDAAKQIL 14 MP1S-D8N No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 89.4% α-helical content in a 25 mM potassium phosphate buffer solution at 20℃ ①Helical contents of all stapled analogues were increased by more than a three-fold compared to their unmodified counterpart, MP1. ②MP1S-D8N and MP1S-Q12K showed slightly lower helical contents (89.4% and 81.8, respectively). Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC = 0.8 μg/mL), Staphylococcus aureus (MIC = 0.8 μg/mL), Staphylococcus epidermidis (MIC = 37.5 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 50 μg/mL), Shigella dysenteriae (MIC = 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC = 37.5 μg/mL), Pseudomonas aeruginosa (MIC ≥ 100 μg/mL) [Ref.29075946] It has 16.3% hemolysis against human red blood cells at 12.5 μM and 37.8% hemolysis at 25 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 29075946 Arch Pharm Res. 2017 Dec;40(12):1414-1419. doi: 10.1007/s12272-017-0963-5. Epub 2017 Oct 26. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity and stability of stapled helices of polybia-MP1 Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CC(C)C)C(=O)NC(CC(=O)N)C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CC(C)C)C(CC)C)CCC(=O)N)CCCC[NH3+])(C)CCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])Cc1c2c([nH]c1)cccc2)CC(=O)[O-])C([NH3+])C(CC)C DRAMP21568 IDWKKⓍLDAⓍKKIL IDWKKXLDAXKKIL IDWKKLLDAAKQIL 14 MP1S-Q12K No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+ 81.8% α-helical content in a 25 mM potassium phosphate buffer solution at 20℃ ①Helical contents of all stapled analogues were increased by more than a three-fold compared to their unmodified counterpart, MP1. ②MP1S-D8N and MP1S-Q12K showed slightly lower helical contents (89.4% and 81.8, respectively). Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC = 0.8 μg/mL), Staphylococcus aureus (MIC = 1.2 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC >100 μg/mL), Shigella dysenteriae (MIC > 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC > 100 μg/mL), Pseudomonas aeruginosa (MIC > 100 μg/mL) [Ref.29075946] It has 9.6% hemolysis against human red blood cells at 12.5 μM and 13.9% hemolysis at 25 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 29075946 Arch Pharm Res. 2017 Dec;40(12):1414-1419. doi: 10.1007/s12272-017-0963-5. Epub 2017 Oct 26. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity and stability of stapled helices of polybia-MP1 Stapled AMP O=C([NH3+])C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C1(C)NC(=O)C(C)NC(=O)C(CC(=O)[O-])NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])C(CC)C)CC(=O)[O-])Cc2c3c([nH]c2)cccc3)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)CCCC[NH3+])CCCC[NH3+])C(CC)C)CC(C)C DRAMP21569 KⓍWKAⓍK KXWKAXK / 7 ALA No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 25 μg/mL), Staphylococcus epidermidis (MIC = 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 25 μg/mL), Shigella dysenteriae (MIC = 50 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 25 μg/mL), Pseudomonas aeruginosa (MIC = 25 μg/mL) [Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21570 KⓍWKLⓍK KXWKLXK / 7 LEU No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 12.5 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 12.5 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 50 μg/mL), Klebsiella pneumoniae (MIC = 18.8 μg/mL), Pseudomonas aeruginosa (MIC = 18.8 μg/mL) [Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21571 KⓍWKVⓍK KXWKVXK / 7 VAL No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ Compared to LEU, the previously reported stapled heptapeptide containing leucine in position 5, peptides VAL and ILE, carrying valine and isoleucine, respectively, appeared slightly more helical as determined by the CD signal intensity at 222 nm. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 18.8 μg/mL), Staphylococcus aureus (MIC = 12.5 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 18.8 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 18.8 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL) [Ref.28547390] It has 1.3% hemolysis against human red blood cells at 25 μM and 2.5% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21572 KⓍWKIⓍK KXWKIXK / 7 ILE No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ Compared to LEU, the previously reported stapled heptapeptide containing leucine in position 5, peptides VAL and ILE, carrying valine and isoleucine, respectively, appeared slightly more helical as determined by the CD signal intensity at 222 nm. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 25 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 25 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 37.5 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL) [Ref.28547390] It has 1.5% hemolysis against human red blood cells at 25 μM and 3.0% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(C(CC)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21573 KⓍWKFⓍK KXWKFXK / 7 PHE No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ On the other hand, peptide PHE, TRP, and GLU, bearing phenylalanine, tryptophan, and glutamate, respectively, showed a slightly decreased helicity. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 25 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 25 μg/mL), Shigella dysenteriae (MIC = 50 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 25 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL) [Ref.28547390] It has 1.9% hemolysis against human red blood cells at 25 μM and 4.4% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(Cc2ccccc2)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21574 KⓍWKWⓍK KXWKWXK / 7 TRP No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ On the other hand, peptide PHE, TRP, and GLU, bearing phenylalanine, tryptophan, and glutamate, respectively, showed a slightly decreased helicity. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 12.5 μg/mL), Staphylococcus aureus (MIC = 12.5 μg/mL), Staphylococcus epidermidis (MIC = 25 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 12.5 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 50 μg/mL), Klebsiella pneumoniae (MIC = 12.5 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL) [Ref.28547390] It has 3.2% hemolysis against human red blood cells at 25 μM and 5.9% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21575 KⓍWKEⓍK KXWKEXK / 7 GLU No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ On the other hand, peptide PHE, TRP, and GLU, bearing phenylalanine, tryptophan, and glutamate, respectively, showed a slightly decreased helicity. Not found Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 100 μg/mL. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC > 100 μg/mL), Staphylococcus aureus (MIC > 100 μg/mL), Staphylococcus epidermidis (MIC > 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 37.5 μg/mL), Shigella dysenteriae (MIC > 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC = 50 μg/mL), Pseudomonas aeruginosa (MIC = 25 μg/mL) [Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCC(=O)[O-])C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21576 KⓍWKKⓍK KXWKKXK / 7 LYS No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ Peptide NLE and LYS maintained a compatible helicity to LEU. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 100 μg/mL), Staphylococcus aureus (MIC = 50 μg/mL), Staphylococcus epidermidis (MIC > 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 50 μg/mL), Shigella dysenteriae (MIC = 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC = 75 μg/mL), Pseudomonas aeruginosa (MIC = 6.3 μg/mL) [Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28547390 Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25. Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim Mono-substitution effects on antimicrobial activity of stapled heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21578 WWVⓍARAⓍRR WWVXARAXRR WWVXARAXRR 10 Val-HSLP No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 0.0% α-helix and 34.3% β-strand in 20 mM potassium phosphate buffer; 0.8% α-helix and 36.9% β-strand in 20 mM potassium phosphate buffer made 30% in TFE. Only slghtly higher β-strand characteristics were observed for the hydrocarbon-stapled peptides in 30% TFE. Overall, the peptides showed some β-strand secondary structural characteristics and little α-helical content. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 20 μM. [Ref.28073163] Gram-positive bacteria: Bacillus megaterium ATCC 14581 (IC50 = 0.69 μM, MIC = 5.00 μM), Staphylococcus aureus ATCC 6538 (IC50 = 0.63 μM, MIC = 5.00 μM), Enterococcus faecalis ATCC 29212 (IC50 = 0.65 μM, MIC = 5.00 μM);##Gram-negative bacteria: Escherichia coli ATCC 700926 (IC50 = 14.8 μM, MIC > 20 μM);## Fungi: Candida albicans 002 ATCC 64385 (IC50 > 20 μM, MIC > 20 μM), C. albicans 004 ATCC MYA-2876 (IC50 > 20 μM, MIC > 20 μM). [Ref.28073163] HC50 = 14.5 μM against human red blood cells. Note: HC50 is the half-maximal hemolytic concentration. Cyclic (Stapled) Acylation (Valerylamide) Amidation ①The Ⓧ (position: 4 and 8) in sequence indicates (S)-2-(4'-pentenyl)-alanine. ②Ⓧ (4) and Ⓧ (8) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28073163 Biopolymers. 2017 May;108(3). doi: 10.1002/bip.23006. Zachary B Jenner, Christopher M Crittenden, Martín Gonzalez, Jennifer S Brodbelt, Kerry A Bruns Hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(CCCNC(=[NH2+])N)C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCNC(=[NH2+])N)CCCNC(=[NH2+])N)(C)CCCC=CCCC1)C(NC(=O)C(NC(=O)C(NC(=O)C)Cc1c2c([nH]c1)cccc2)Cc1c2c([nH]c1)cccc2)C(C)C.CC.[H+H0].[H+H0].[H+H0] DRAMP21580 WWVⓍAFAⓍRRR WWVXAFAXRRR WWVXAFAXRRR 11 Cap-HSLP No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+ 0.3% α-helix and 31.9% β-strand in 20 mM potassium phosphate buffer; 0.7% α-helix and 40.0% β-strand in 20 mM potassium phosphate buffer made 30% in TFE. Only slghtly higher β-strand characteristics were observed for the hydrocarbon-stapled peptides in 30% TFE. Overall, the peptides showed some β-strand secondary structural characteristics and little α-helical content. Not found Function: Antibacterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria and antifungal activity against Candida albicans are not noteable under 20 μM. [Ref.28073163] Gram-positive bacteria: Bacillus megaterium ATCC 14581 (IC50 = 1.63 μM, MIC = 5.00 μM), Staphylococcus aureus ATCC 6538 (IC50 = 0.64 μM, MIC = 5.00 μM), Enterococcus faecalis ATCC 29212 (IC50 = 0.63 μM, MIC = 1.25 μM);##Gram-negative bacteria: Escherichia coli ATCC 700926 (IC50 = 169 μM, MIC > 20 μM);## Fungi: Candida albicans 002 ATCC 64385 (IC50 > 20 μM, MIC > 20 μM), C. albicans 004 ATCC MYA-2876 (IC50 > 20 μM, MIC > 20 μM). [Ref.28073163] HC50 = 4.49 μM against human red blood cells. Note: HC50 is the half-maximal hemolytic concentration. Cyclic (Stapled) Acylation (Caproylamide) Amidation ①The Ⓧ (position: 4 and 8) in sequence indicates (S)-2-(4'-pentenyl)-alanine. ②Ⓧ (4) and Ⓧ (8) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 28073163 Biopolymers. 2017 May;108(3). doi: 10.1002/bip.23006. Zachary B Jenner, Christopher M Crittenden, Martín Gonzalez, Jennifer S Brodbelt, Kerry A Bruns Hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(Cc2ccccc2)C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCNC(=[NH2+])N)CCCNC(=[NH2+])N)(C)CCCC=CCCC1)C(NC(=O)C(NC(=O)C(NC(=O)C)Cc1c2c([nH]c1)cccc2)Cc1c2c([nH]c1)cccc2)C(C)C.C(C)C.[H+H0].[H+H0].[H+H0] DRAMP21582 KⓍWKLⓍK KXWKLXK / 7 S3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- Stable α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 25 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 100 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 50 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 100 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 50 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 75 μg/mL) It has <1% hemolysis against human red blood cells at 6.3 μM and <1% hemolysis at 12.5 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21583 KⓍWKLⓍKGKⓍWKLⓍK KXWKLXKGKXWKLXK / 15 3GL3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ 3GL3 apperaed to be the most helical in this series as indicated by the distinct double minima near at 208 and 222 nm with similar intensities. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 2.3 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 1.6 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 3.1 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 37.5 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 50 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 6.3 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 9.4 μg/mL) It has 19.1% hemolysis against human red blood cells at 6.3 μM and 31.8% hemolysis at 12.5 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC2(C)C(=O)NC(Cc3c4c([nH]c3)cccc4)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC2)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21584 KⓍWKLⓍKAKⓍWKLⓍK KXWKLXKAKXWKLXK / 15 3BA3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- No specific results about the strcture presented in the forms of tables, graphs or words No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 2.3 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 1.6 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 3.1 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 6.3 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 12.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 3.1 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.1 μg/mL) It has 14.2% hemolysis against human red blood cells at 6.3 μM and 24.9% hemolysis at 12.5 μM. Cyclic (Stapled) Acetylation Amidation ①The A (position: 8) in sequence is β-Ala. ②The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC2(C)C(=O)NC(Cc3c4c([nH]c3)cccc4)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC2)CCCC[NH3+])C)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21585 KⓍWKLⓍKBKⓍWKLⓍK KXWKLXKBKXWKLXK / 15 3GA3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- No specific results about the strcture presented in the forms of tables, graphs or words No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 4.0 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 3.1 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.7 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 18.8 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 37.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 9.4 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 9.4 μg/mL) It has 12.9% hemolysis against human red blood cells at 6.3 μM and 24.4% hemolysis at 12.5 μM. Cyclic (Stapled) Acetylation Amidation ①The B (position: 8) in sequence is γ-aminobutyric acid (GABA). ②The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)NCCCC(=O)NC(C(=O)NC2(C)C(=O)NC(Cc3c4c([nH]c3)cccc4)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)[NH-])CCCC[NH3+])(C)CCCC=CCCC2)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21586 KⓍWKLⓍKPKⓍWKLⓍK KXWKLXKPKXWKLXK / 15 3PR3-X No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ "Whereas all other dimeric analogs were obtained as a single exclusive product, the proline-containing sequence yielded two products (3PR3-X and 3PR3-Y) in similar amounts. These might be conformational isomers induced by the cis–trans configuration of the proline linker. 3PR3-X, which showed the weakest hemolytic activity, displayed a CD spectrum similar to that of the monomeric S3 and the lowest helical content in this series." Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 3.1 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 4.7 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 6.3 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 12.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 4.7 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 9.4 μg/mL) It has 5.7% hemolysis against human red blood cells at 6.3 μM and 9.7% hemolysis at 12.5 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)N2C(C(=O)NC(C(=O)NC3(C)C(=O)NC(Cc4c5c([nH]c4)cccc5)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC3)CCCC[NH3+])CCC2)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21587 KⓍWKLⓍKPKⓍWKLⓍK KXWKLXKPKXWKLXK / 15 3PR3-Y No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃ "Whereas all other dimeric analogs were obtained as a single exclusive product, the proline-containing sequence yielded two products (3PR3-X and 3PR3-Y) in similar amounts. These might be conformational isomers induced by the cis–trans configuration of the proline linker." Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.2 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 1.2 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 4.7 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.3 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 4.7 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 12.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 3.1 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.3 μg/mL) It has 16.2% hemolysis against human red blood cells at 6.3 μM and 31.9% hemolysis at 12.5 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. ③The P (position: 8) in sequence is D-proline. Mixed (D-Pro8) No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839. Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers Stapled AMP DRAMP21588 KⓍWKAⓍK KXWKAXK / 7 Ac-S1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5) On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 37.5 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21589 KⓍWKAⓍK KXWKAXK / 7 H-S1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5) On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 37.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 25 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP DRAMP21590 KⓍAKWⓍK KXAKWXK / 7 Ac-S2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5) Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 37.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21591 KⓍAKWⓍK KXAKWXK / 7 H-S2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix (but less helical than Ac-S2) in a 25 mM potassium phosphate buffer solution (pH 6.5) Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 37.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 37.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 37.5 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP DRAMP21592 KⓍWKLⓍK KXWKLXK / 7 Ac-S3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5) On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 18.8 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 75 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, 1.59% and 5.53% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21593 KⓍWKLⓍK KXWKLXK / 7 H-S3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5) ①On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap. ②It should be also noted that H-S3 displays a CD spectrum that is typically observed from α-helical peptides even without the N-acetyl cal. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 12.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 25 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and 2.05% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP DRAMP21594 KⓍLKWⓍK KXLKWXK / 7 Ac-S4 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5) Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, 1.29%, 2.84% and 5.74% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP O=C(NC1(C)C(=O)NC(CC(C)C)C(=O)NC(CCCC[NH3+])C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21595 KⓍLKWⓍK KXLKWXK / 7 H-S4 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix (but less helix content Ac-S4) in a 25 mM potassium phosphate buffer solution (pH 6.5) Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 50 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 75 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 25 μM) It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483. Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities Stapled AMP DRAMP21597 KⓍAKAⓍKKAAKAAWK KXAKAXKKAAKAAWK KAAKAAKKAAKAAWK 15 Ac-SS-14W No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH6.5) On the other hand, singly-stapled analog Ac-SS-14W exhibited a typical CD spectrum for α-helix, characterized by two minima near 208 and 222 nm and a maximum near 190 nm. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 9.4 μM), Staphylococcus epidermis ATCC 12228 (MIC = 75 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μM), Shigella dysentariae ATCC 9752 (MIC = 18.8 μM), Salmonella typhimurium ATCC 14028 (MIC > 200 μM), Klebsiella pneumonia ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM). [Ref.26235946] It has <1% hemolysis against human red blood cells at 12.5 μM and <1% hemolysis at 25 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 26235946 Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19. Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity of doubly-stapled alanine/lysine-based peptides Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])Cc2c3c([nH]c2)cccc3)C)C)CCCC[NH3+])C)C)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21598 KⓍAKAⓍKKⓍAKAⓍWK KXAKAXKKXAKAXWK KAAKAAKKAAKAAWK 15 Ac-DS-14W No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH6.5) Doubly-stapled Ac-Ds-14W showed the most enhaced helical contents among this series of peptides. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 4.8 μM), Staphylococcus epidermis ATCC 12228 (MIC = 3.1 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 200 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM). [Ref.26235946] It has 22.1% hemolysis against human red blood cells at 12.5 μM and 38.8% hemolysis at 25 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 26235946 Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19. Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity of doubly-stapled alanine/lysine-based peptides Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC2(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])Cc3c4c([nH]c3)cccc4)(C)CCCC=CCCC2)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21599 KⓍAKAⓍKKⓍAKWⓍAK KXAKAXKKXAKWXAK KAAKAAKKAAKAAWK 15 Ac-DS-12W No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) In addition, the positional modification of tryptophan caused significant changes in the conformation: in the CD analysis, the most active Ac-DS-5W exhibited markedly enhanced helical content whereas the equipotent Ac-DS-12W showed decreased helicity compared to Ac-DS-14W Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 3.1 μM), Staphylococcus epidermis ATCC 12228 (MIC = 4.8 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 200 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM). [Ref.26235946] It has 25.3% hemolysis against human red blood cells at 12.5 μM and 42.5% hemolysis at 25 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 26235946 Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19. Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity of doubly-stapled alanine/lysine-based peptides Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC2(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(Cc3c4c([nH]c3)cccc4)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C)(C)CCCC=CCCC2)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21600 KⓍAKWⓍKKⓍAKAⓍAK KXAKWXKKXAKAXAK KAAKAAKKAAKAAWK 15 Ac-DS-3W No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) No other descriptive information about the structure found in the literature Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 3.1 μM), Staphylococcus aureus ATCC 6538p (MIC = 4.8 μM), Staphylococcus epidermis ATCC 12228 (MIC = 4.8 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 200 μM). [Ref.26235946] It has 28.9% hemolysis against human red blood cells at 12.5 μM and 42.5% hemolysis at 25 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 26235946 Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19. Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity of doubly-stapled alanine/lysine-based peptides Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC2(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C)(C)CCCC=CCCC2)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21601 KⓍWKAⓍKKⓍAKAⓍAK KXWKAXKKXAKAXAK KAAKAAKKAAKAAWK 15 Ac-DS-5W No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) In addition, the positional modification of tryptophan caused significant changes in the conformation: in the CD analysis, the most active Ac-DS-5W exhibited markedly enhanced helical content whereas the equipotent Ac-DS-12W showed decreased helicity compared to Ac-DS-14W Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 1.6 μM), Staphylococcus epidermis ATCC 12228 (MIC = 1.6 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 18.8 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 37.5 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM). [Ref.26235946] It has 22.0% hemolysis against human red blood cells at 12.5 μM and 38.5% hemolysis at 25 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 26235946 Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19. Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity of doubly-stapled alanine/lysine-based peptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC2(C)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C)C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C)(C)CCCC=CCCC2)CCCC[NH3+])CCCC[NH3+])(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21602 KⓍAKWⓍKKⓍAKAⓍAK KXAKWXKKXAKAXAK KAAKAAKKAAKAAWK 15 Su-DS-5W No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) In the CD experiments, these new analogs showed similar helicity to Ac-DS-5W although Su-DS-5W displayed a slight increase in helicity whereas H-DS-5W exhibited a slight decrease. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 1.6 μM), Staphylococcus epidermis ATCC 12228 (MIC = 1.6 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC > 200 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 50 μM). [Ref.26235946] It has 19.5% hemolysis against human red blood cells at 12.5 μM and 32.6% hemolysis at 25 μM. Cyclic (Stapled) Succinylation Amidation ①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 26235946 Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19. Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity of doubly-stapled alanine/lysine-based peptides Stapled AMP DRAMP21603 KⓍAKWⓍKKⓍAKAⓍAK KXAKWXKKXAKAXAK KAAKAAKKAAKAAWK 15 H-DS-5W No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) In the CD experiments, these new analogs showed similar helicity to Ac-DS-5W although Su-DS-5W displayed a slight increase in helicity whereas H-DS-5W exhibited a slight decrease. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 1.6 μM), Staphylococcus epidermis ATCC 12228 (MIC = 1.6 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μM), Shigella dysentariae ATCC 9752 (MIC = 12.5 μM), Salmonella typhimurium ATCC 14028 (MIC = 6.3 μM), Klebsiella pneumonia ATCC 10031 (MIC = 12.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.3 μM). [Ref.26235946] It has 13.5% hemolysis against human red blood cells at 12.5 μM and 25.5% hemolysis at 25 μM. Cyclic (Stapled) Free Amidation ①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found in the reference 26235946 Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19. Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim Antimicrobial activity of doubly-stapled alanine/lysine-based peptides Stapled AMP DRAMP21605 KⓍWKAⓍK KXWKAXK KXWKAXK 7 S1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 50 μM). It has 0.53%, 0.53%, 0.67%, 0.66%, 0.83%, 1.36%, 1.17% and 1.21% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides Stapled AMP DRAMP21607 KⓍAKWⓍK KXAKWXK KXAKWXK 7 S2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 50 μM), Staphylococcus aureus ATCC 6538p (MIC = 100 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM). It has 0.91%, 1.01%, 0.66%, 0.99%, 0.63%, 0.82%, 1.75% and 1.24% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides Stapled AMP DRAMP21609 KⓍWKLⓍK KXWKLXK KXWKLXK 7 S3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylococcus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM). It has 0.87%, 0.65%, 1.02%, 0.94%, 2.13%, 2.52%, 3.81% and 7.75% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides Stapled AMP DRAMP21611 KⓍLKWⓍK KXLKWXK KXLKWXK 7 S4 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 12.5 μM), Staphylococcus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM). It has 0.71%, 0.90%, 0.71%, 0.68%, 1.08%, 1.87%, 2.46% and 4.50% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides Stapled AMP DRAMP21613 KⓍWAKⓍA KXWAKXA KXWAKXA 7 S5 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 50 μM), Staphylococcus aureus ATCC 6538p (MIC = 50 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM). It has 0.65%, 0.60%, 0.59%, 0.76%, 0.83%, 0.76%, 0.88% and 1.04% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C=O)C)(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21615 KⓍAWKⓍA KXAWKXA KXAWKXA 7 S6 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5) As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts. Not found Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 100 μM. Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC > 100 μM), Staphylococcus aureus ATCC 6538p (MIC > 100 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 100 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC > 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM). It has 0.65%, 0.66%, 0.75%, 0.87%, 0.64%, 0.70%, 0.61% and 0.84% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. L No cytotoxicity information found. PubMed ID is not available B KOREAN CHEM SOC Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides Stapled AMP O=C(NC1(C)C(=O)NC(C)C(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(CCCC[NH3+])C(=O)NC(C(=O)NC(C=O)C)(C)CCCC=CCCC1)C([NH3+])CCCC[NH3+] DRAMP21617 qqrkrkiwsⓚlapⓓgttlvklvagig qqrkrkiwsklapdgttlvklvagig qqrkrkiwsilaplgttlvklvagig 26 sDRIM No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①Disordered (or unstructured) conformation in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②50% average α-helix content in various membranes environments [57% in POPC; 67% in POPC/P In the cases of sDRIM and sKFGF, stapling did not induce any conformational change, as these analogues remained just as unstructured. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC = 128 μg/mL), Enterococcus faecalis (MIC = 64 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 32 μg/mL), Pseudomonas aeruginosa (MIC = 64 μg/mL) [Ref.28921993] It has 13.4%, 18.6%, 25.4%, 33.4%, 42.0%, 46.9%, 42.2% and 47.1% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml. Cyclic (Stapled) Free Amidation ⓚ (10) and ⓓ (14) are corss-linked by lactam stapling through the polar amide bond of a lactam bridge. D [Ref.28921993] The toxicity of sDRIM toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM. 28921993 J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26. Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties. Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NCC=O)C(CC)C)C)C(C)C)CC(C)C)CCCC[NH3+])C(C)C)CC(C)C)C(O)C)C(NC(=O)CNC(=O)C(NC(=O)C1N(C(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])CCC(=O)N)CCC(=O)N)CCCNC(=[NH2+])N)CCCC[NH3+])CCCNC(=[NH2+])N)CCCC[NH3+])C(CC)C)Cc2c3c([nH]c2)cccc3)CO)CCCC[NH3+])CC(C)C)C)CCC1)CC=O)C(O)C DRAMP21619 PLIⓀLRLⒹRGQF PLIKLRLDRGQF PLILLRLLRGQF 12 sWWSP No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①α-helix in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②43% average α-helix content in various membranes environments [38% in POPC; 58% in POPC/POPG(3:1); 47% in POPC:lysoPC(9:1); On the other hand, sWWSP and sMAP-1 assumed an α-helical conformation in aqueous solution, in contrast to their unstructured linear countparts. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC > 256 μg/mL), Enterococcus faecalis (MIC > 256 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 256 μg/mL), Pseudomonas aeruginosa (MIC > 256 μg/mL) [Ref.28921993] It has 4.0%, 4.8%, 4.6%, 4.2% and 7.0% hemolysis against human red blood cells at 15, 20, 25, 30 and 40 μg/ml. Cyclic (Stapled) Free Amidation Ⓚ (4) and Ⓓ (8) are cross-linked by lactam stapling through the polar amide bond of a lactam bridge. L [Ref.28921993] The toxicity of sWWSP toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM. 28921993 J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26. Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties. Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC(C=O)Cc1ccccc1)CCC(=O)N)CCCNC(=[NH2+])N)CC=O)CC(C)C)CCCNC(=[NH2+])N)CC(C)C)CCCC[NH3+])C(NC(=O)C(NC(=O)C1[NH2+]CCC1)CC(C)C)C(CC)C DRAMP21621 AAⓀLLPⒹLLAAP AAKLLPDLLAAP AAVLLPVLLAAP 12 sKFGF No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①Disordered (or unstructured) conformation in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②Around 22% average α-helix content in various membranes environments [13% in POPC; 32% in In the cases of sDRIM and sKFGF, stapling did not induce any conformational change, as these analogues remained just as unstructured. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC > 256 μg/mL), Enterococcus faecalis (MIC > 256 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 256 μg/mL), Pseudomonas aeruginosa (MIC > 256 μg/mL) [Ref.28921993] It has 0.4%, 0%, 0.1%, 0.2%, 0.1%, 0.7%, 1.8% and 5.6% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml. Cyclic (Stapled) Free Amidation Ⓚ (3) and Ⓓ (7) are cross-linked by lactam stapling through the polar amide bond of a lactam bridge. L [Ref.28921993] The toxicity of sKFGF toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM. 28921993 J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26. Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties. Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)N1C(C=O)CCC1)C)C)CC(C)C)CC(C)C)C(NC(=O)C1N(C(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C([NH3+])C)C)CCCC[NH3+])CC(C)C)CC(C)C)CCC1)CC=O DRAMP21623 KLALKALKⓀLKAⒹLKLA KLALKALKKLKADLKLA KLALKALKALKAALKLA 17 sMAP-1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- ①α-helix in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②46% average α-helix content in various membranes environments [37% in POPC; 59% in POPC/POPG(3:1); 45% in POPC:lysoPC(9:1); On the other hand, sWWSP and sMAP-1 assumed an α-helical conformation in aqueous solution, in contrast to their unstructured linear countparts. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC = 64 μg/mL), Enterococcus faecalis (MIC > 256 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 256 μg/mL), Pseudomonas aeruginosa (MIC > 256 μg/mL) [Ref.28921993] It has 2.5%, 2.9%, 2.4%, 4.1%, 2.9%, 6.3%, 4.9% and 8.2% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml. Cyclic (Stapled) Free Amidation Ⓚ (9) and Ⓓ (13) are cross-linked by lactam stapling through the polar amide bond of a lactam bridge. L [Ref.28921993] The toxicity of sMAP-1 toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM. 28921993 J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26. Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties. Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)C)CC(C)C)CCCC[NH3+])CC(C)C)CC=O)C)CCCC[NH3+])CC(C)C)CCCC[NH3+])CCCC[NH3+])CC(C)C)C)CCCC[NH3+])CC(C)C)C)CC(C)C)C([NH3+])CCCC[NH3+] DRAMP21624 ⓍLAAⒿRHⓍ XLAAJRHX ELAAIRHR 8 V30-SP-8 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+ 26.1% α-helix content in 50 μM aqueous solution [a mixture of water and acetonitrile (9:1, v/v)]. ①The helical propensities of two stapled peptides, V30-SP-8 and V30-SP-9, and their linear counterparts were analyzed via circular dichroism (CD) spectrometry. ②Surprisingly, in contrast to our expectation that the stitched peptide, V30-SP-8, would be more helical than the monostapled peptide, V30-SP-9, the monostapling strategy was more effective in inducing helicity (helicity: 26.1% for V30-SP-8 and 33.8% for V30-SP-9) Not found Function: Antibacterial activity against Gram-positive bacteria. The peptide shows a similar potency to vancomycin (MIC50 = 20 μM against M.smegmatis) [Ref.32840352] Gram-positive bacteria: Mycobacterium smegmatis (The antibacterial effects of V30-SP-8 are 36.9%, 42.7%, 50.9% and 55.4% at 6.25, 12.5, 25 and 50 μM, and MIC50 is between 12.5 μM and 25.0 μM) [Ref.32840352] No hemolytic activity information found. Cyclic (Stapled) Free Free ①The Ⓧ (position: 1 and 8) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓙ (position: 5) in sequence is B5 stapling amino acid. Note: B5 is α-dipentenyl alanine. ③Ⓧ (1) and Ⓙ (5), Ⓙ (5) and Ⓧ (8) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple respectively. L No cytotoxicity information found in the reference 32840352 ACS Chem Biol. 2020 Sep 18;15(9):2493-2498. doi: 10.1021/acschembio.0c00492. Epub 2020 Sep 9. Sung-Min Kang, Heejo Moon, Sang-Woo Han, Do-Hee Kim, Byeong Moon Kim, Bong-Jin Lee Structure-Based De Novo Design of Mycobacterium Tuberculosis VapC-Activating Stapled Peptides Stapled AMP O=C([O-])C1(C)NC(=O)C(Cc2nc[nH]c2)NC(=O)C(CCCNC(=[NH2+])N)NC(=O)C2(NC(=O)C(C)NC(=O)C(C)NC(=O)C(CC(C)C)NC(=O)C([NH3+])(C)CCCC=CCCC2)CCCC=CCCC1 DRAMP21625 ⓏLAAIRHⓍ ZLAAIRHX ELAAIRHR 8 V30-SP-9 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+ 33.8% α-helix content in 50 μM aqueous solution [a mixture of water and acetonitrile (9:1, v/v)]. ①The helical propensities of two stapled peptides, V30-SP-8 and V30-SP-9, and their linear counterparts were analyzed via circular dichroism (CD) spectrometry. ②Surprisingly, in contrast to our expectation that the stitched peptide, V30-SP-8, would be more helical than the monostapled peptide, V30-SP-9, the monostapling strategy was more effective in inducing helicity (helicity: 26.1% for V30-SP-8 and 33.8% for V30-SP-9) Not found Function: Antibacterial activity against Gram-positive bacteria. [Ref.32840352] Gram-positive bacteria: Mycobacterium smegmatis (The antibacterial effects of V30-SP-8 are 23.3%, 23.3%, 34.2% and 38.7% at 6.25, 12.5, 25 and 50 μM, and MIC50 is above 25 μM) [Ref.32840352] No hemolytic activity information found. Cyclic (Stapled) Free Free ①The Ⓩ (position: 1) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ②The Ⓧ (position: 8) in sequence is (S)-pentenyl alanine. ③Ⓩ (1) and Ⓧ (8) are cross-linked by hydrocarbon stapling through a undec-4-enyl staple. L No cytotoxicity information found in the reference 32840352 ACS Chem Biol. 2020 Sep 18;15(9):2493-2498. doi: 10.1021/acschembio.0c00492. Epub 2020 Sep 9. Sung-Min Kang, Heejo Moon, Sang-Woo Han, Do-Hee Kim, Byeong Moon Kim, Bong-Jin Lee Structure-Based De Novo Design of Mycobacterium Tuberculosis VapC-Activating Stapled Peptides Stapled AMP O=C([O-])C1(C)NC(=O)C(Cc2nc[nH]c2)NC(=O)C(CCCNC(=[NH2+])N)NC(=O)C(C(CC)C)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CC(C)C)NC(=O)C([NH3+])(C)CCCCCCC=CCCC1 DRAMP21628 TLKQFⓍKGVⓍKWLVK TLKQFXKGVXKWLVK TLKQFAKGVGKWLVK 15 E2EM15W-S1 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+ 47% α-helical content in a 25 mM potassium phosphate buffer solution at 20 ℃. ①On the other hand, all three stapled analogs of E2EM15W showed substantial increases in helical contents, which again demonstrated the highly effective helix-stabilization through the all-hydrocarbon stapling technology. ②E2EM15W-S1, the most potent analog in the antimicrobial assay, showed the highest degree of helicity (47%) in the aqueous solution, supporting a close correlation between the helicity and the antimicrobial activity of the peptides in this series. Not found Function: Antibcaterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria is not noteable under 200 μg/mL. [Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 3.13 μg/mL), Staphylococcus aureus (MIC = 3.13 μg/mL), Staphylococcus epidermis (MIC > 200 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 200 μg/mL), Shigella dysentariae (MIC > 200 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC > 200 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC > 200 μg/mL). [Ref.24211019] No hemolytic activity information found. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl staple. L No cytotoxicity information found in the reference 24211019 Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26. Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Truncated and constrained helical analogs of antimicrobial esculentin-2EM Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NCC(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C(C)C)CC(C)C)Cc2c3c([nH]c2)cccc3)CCCC[NH3+])(C)CCCC=CCCC1)Cc1ccccc1)CCC(=O)N)CCCC[NH3+])CC(C)C)C([NH3+])C(O)C DRAMP21629 TLKQFⓍKGWⓍKDLVK TLKQFXKGWXKDLVK TLKQFAKGVGKWLVK 15 E2EM15W-S2 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 27% α-helical content in a 25 mM potassium phosphate buffer solution at 20 ℃. ①On the other hand, all three stapled analogs of E2EM15W showed substantial increases in helical contents, which again demonstrated the highly effective helix-stabilization through the all-hydrocarbon stapling technology. ②Although they bear the oce-4-enyl staple at the same positions as in E2EM15W-S1, the other stapled derivatives E2EM15W-S2 and E2EM15W-S3 exhibited markedly smaller helical contents: 27% and 37%, repectively. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 6.25 μg/mL), Staphylococcus aureus (MIC = 6.25 μg/mL), Staphylococcus epidermis (MIC > 200 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 100 μg/mL), Shigella dysentariae (MIC = 50 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC = 50 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC = 200 μg/mL). [Ref.24211019] No hemolytic activity information found. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl staple. L No cytotoxicity information found in the reference 24211019 Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26. Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Truncated and constrained helical analogs of antimicrobial esculentin-2EM Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NCC(=O)NC(Cc2c3c([nH]c2)cccc3)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C(C)C)CC(C)C)CC(=O)[O-])CCCC[NH3+])(C)CCCC=CCCC1)Cc1ccccc1)CCC(=O)N)CCCC[NH3+])CC(C)C)C([NH3+])C(O)C DRAMP21630 TLKQWⓍKGVⓍKDLVK TLKQWXKGVXKDLVK TLKQFAKGVGKWLVK 15 E2EM15W-S3 No entry found Synthetic construct Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- 37% α-helical content in a 25 mM potassium phosphate buffer solution at 20 ℃. ①On the other hand, all three stapled analogs of E2EM15W showed substantial increases in helical contents, which again demonstrated the highly effective helix-stabilization through the all-hydrocarbon stapling technology. ②Although they bear the oce-4-enyl staple at the same positions as in E2EM15W-S1, the other stapled derivatives E2EM15W-S2 and E2EM15W-S3 exhibited markedly smaller helical contents: 27% and 37%, repectively. Not found Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. [Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 6.25 μg/mL), Staphylococcus aureus (MIC = 6.25 μg/mL), Staphylococcus epidermis (MIC = 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 100 μg/mL), Shigella dysentariae (MIC = 50 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC = 50 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC > 200 μg/mL). [Ref.24211019] No hemolytic activity information found. Cyclic (Stapled) Acetylation Amidation ①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl staple. L No cytotoxicity information found in the reference 24211019 Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26. Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim Truncated and constrained helical analogs of antimicrobial esculentin-2EM Stapled AMP O=C(NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC1(C)C(=O)NC(CCCC[NH3+])C(=O)NCC(=O)NC(C(C)C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C=O)CCCC[NH3+])C(C)C)CC(C)C)CC(=O)[O-])CCCC[NH3+])(C)CCCC=CCCC1)Cc1c2c([nH]c1)cccc2)CCC(=O)N)CCCC[NH3+])CC(C)C)C([NH3+])C(O)C DRAMP21651 TLDPPYFLDPVSPNPMCHRP 20 SLAY-screened peptide P1 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTTGACCCTCCCTATTTTCTCGATCCTGTCAGCCCCAATCCCATGTGCCACCGTCCCTAA TLDPPYFLDPVSPNPMCHRP* -11.199 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21652 LQSPDLQHFQYLLLLSGSRGL 21 SLAY-screened peptide P2 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCAGAGCCCTGACTTACAGCATTTTCAATACTTATTACTGTTATCAGGGTCCCGGGGTCTA LQSPDLQHFQYLLLLSGSRGL -11.079 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21653 QRRH 4 SLAY-screened peptide P3 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCGCCGGCATTAGCTGCACACGAGTTCGCTGTCCCCCAGTCTGGGCAGTTTTTGGGCTTAA QRRH*LHTSSLSPSLGSFWA* -10.843 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21654 AGAAFNSCAR 10 SLAY-screened peptide P4 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGGGGCGGCTTTCAATTCCTGTGCCAGGTAGGATGACAACTTCTACATCTATTATGCGTAA AGAAFNSCAR*DDNFYIYYA* -10.83 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21655 SSHLPHHGCNRRFVDGPAPPQ 21 SLAY-screened peptide P5 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTCGCACCTTCCCCATCACGGTTGTAACCGCCGTTTTGTGGACGGCCCCGCCCCCCCCCAA SSHLPHHGCNRRFVDGPAPPQ -10.803 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21656 NATMLCLSDNFCNENFTHQA 20 SLAY-screened peptide P6 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGCTACTATGCTCTGCCTTTCCGATAATTTTTGCAACGAGAATTTTACGCATCAGGCCTAA NATMLCLSDNFCNENFTHQA* -10.8 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21657 GDS 3 SLAY-screened peptide P7 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGATAGCTAGTGTTTCAAGTATACTTATCCCTGGAATAATGACTACCACGTTAGCGCGTAA GDS*CFKYTYPWNNDYHVSA* -10.604 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21658 IHPLSFR 7 SLAY-screened peptide P8 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCATCCCCTCTCCTTTCGTTAGTATCGCAACGTTCGGACCATTAATTCGTCCGCTGTGTAA IHPLSFR*YRNVRTINSSAV* -10.57 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21659 WYVFSLAVAPVNNTNRDGSP 20 SLAY-screened peptide P9 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGTACGTCTTCAGTCTCGCCGTCGCGCCTGTTAACAATACGAATCGCGATGGGTCCCCTTAA WYVFSLAVAPVNNTNRDGSP* -10.462 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21660 PSNAVMPINARYKSGYSPAS 20 SLAY-screened peptide P10 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGCAATGCTGTTATGCCCATTAATGCCCGCTACAAGAGCGGTTATTCGCCTGCCTCTTAA PSNAVMPINARYKSGYSPAS* -10.364 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21661 NNLYHTHGNCYKDTNINFEN 20 SLAY-screened peptide P11 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAATCTCTACCACACTCATGGGAATTGCTACAAGGACACCAATATTAATTTTGAGAACTAA NNLYHTHGNCYKDTNINFEN* -10.274 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21662 HLLPVISI 8 SLAY-screened peptide P12 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCTGCTCCCCGTCATCTCTATCTAGCATCAGGTCGTCTCCGTGGGTCATGACGCGCTGTAA HLLPVISI*HQVVSVGHDAL* -10.157 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21663 LTASRAPGSLPTVWLPIVLLN 21 SLAY-screened peptide P13 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACTGCGTCTCGAGCACCGGGGTCATTACCAACTGTTTGGCTCCCTATAGTACTACTTAAC LTASRAPGSLPTVWLPIVLLN -10.141 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21664 IG 2 SLAY-screened peptide P14 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGGTTAGGGTGACCTTTACATCACTGAGACTTAGGATTATAATAGTAGTCTTTTTGATTAA IG*GDLYITET*DYNSSLFD* -10.056 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21665 APMGYSSVASSMSTSSYFID 20 SLAY-screened peptide P15 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCCATGGGTTATTCTTCTGTCGCGTCTAGCATGTCTACTTCTTCTTACTTTATTGACTAA APMGYSSVASSMSTSSYFID* -10.041 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21666 RPRLSGIMTYYVSTWISYIC 20 SLAY-screened peptide P16 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCCCGTCTTAGCGGGATCATGACCTATTACGTTTCCACTTGGATCAGCTACATTTGTTAA RPRLSGIMTYYVSTWISYIC* -10.03 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21667 LSGERRHTVGVQTMHSDHME 20 SLAY-screened peptide P17 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTCTGGTGAGAGGAGGCACACTGTCGGTGTCCAGACCATGCATTCTGATCATATGGAGTAA LSGERRHTVGVQTMHSDHME* -9.883 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21668 QSKPDATQPYVHYCKRRLLR 20 SLAY-screened peptide P18 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTCCAAGCCTGACGCTACTCAGCCTTATGTCCATTACTGCAAGCGTCGTCTCCTGCGTTAA QSKPDATQPYVHYCKRRLLR* -9.861 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21669 PCATALIPSPRQDSRTL 17 SLAY-screened peptide P19 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCGCTACGGCCCTCATTCCTTCGCCTCGGCAGGATTCCCGGACCCTGTAGATTAAGTAA PCATALIPSPRQDSRTL*IK* -9.719 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21670 GCLNFSVPVDRPVSPAKTAW 20 SLAY-screened peptide P20 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTGTCTGAACTTTTCTGTCCCCGTGGACCGGCCTGTGTCGCCTGCCAAGACGGCCTGGTAA GCLNFSVPVDRPVSPAKTAW* -9.619 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21671 RVVILMLS 8 SLAY-screened peptide P21 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGTCGTCATCTTGATGTTGTCCTAGAATGCTTGTCATCAGTTGCATCTGACGATTTCTTAA RVVILMLS*NACHQLHLTIS* -9.558 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21672 FRCPPFKFSCLALAFTDYNN 20 SLAY-screened peptide P22 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCGGTGCCCGCCGTTTAAGTTCTCCTGTCTTGCCCTTGCTTTTACGGATTATAACAATTAA FRCPPFKFSCLALAFTDYNN* -9.504 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21673 PVRCVTPTSPCAPNPHYHDQ 20 SLAY-screened peptide P23 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGTGCGTTGTGTCACGCCTACTTCTCCGTGTGCTCCTAATCCTCACTACCATGATCAGTAA PVRCVTPTSPCAPNPHYHDQ* -9.45 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21674 YAFFINNDCFYYCSLGPCASN 21 SLAY-screened peptide P24 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGCCTTTTTTATCAATAATGATTGTTTTTATTATTGTTCTTTAGGCCCATGTGCATCTAAC YAFFINNDCFYYCSLGPCASN -9.396 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21675 PLAPIVQTYS 10 SLAY-screened peptide P25 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTTGCCCCGATTGTTCAGACGTACTCTTAGAGCACTAATCTGTATCGCGGTTATTGCTAA PLAPIVQTYS*STNLYRGYC* -9.352 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21676 SWHWLSSNHIATVSVETYSH 20 SLAY-screened peptide P26 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTGGCACTGGCTTTCTTCTAATCATATCGCGACCGTGAGCGTTGAGACCTACTCCCACTAA SWHWLSSNHIATVSVETYSH* -9.321 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21677 TLSVFFCIHPPPCSVSTSPY 20 SLAY-screened peptide P27 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTCTCCGTCTTTTTCTGCATCCACCCCCCCCCCTGTAGTGTCTCTACGTCTCCGTATTAA TLSVFFCIHPPPCSVSTSPY* -9.161 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21678 YLLARLALQTFFSHGFYTFP 20 SLAY-screened peptide P28 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCTGCTCGCTCGGCTCGCCCTGCAGACGTTTTTCAGTCACGGTTTCTATACTTTTCCTTAA YLLARLALQTFFSHGFYTFP* -9.158 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21679 LTLLICPDDTFYKAK 15 SLAY-screened peptide P29 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACCTTGCTTATTTGCCCCGATGATACTTTTTATAAGGCGAAGTAGGCCTCTCCTTTTTAA LTLLICPDDTFYKAK*ASPF* -9.154 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21680 NLFPSHSATFRH 12 SLAY-screened peptide P30 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTTGTTCCCGAGCCATTCTGCCACCTTTCGGCATTAGGATTATATTCTGCGTTTTCGTTAA NLFPSHSATFRH*DYILRFR* -9.146 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21681 TSDYILVQFYFS 12 SLAY-screened peptide P31 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTCTGATTACATTTTGGTTCAGTTTTATTTTTCTTAGACGCTCACCCACCTCAACTGTTAA TSDYILVQFYFS*TLTHLNC* -9.104 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21682 PCISQSNDVCPSRESLPLCI 20 SLAY-screened peptide P32 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTGTATCAGCCAGTCGAACGACGTCTGCCCGTCGCGTGAGTCTTTGCCTCTGTGTATTTAA PCISQSNDVCPSRESLPLCI* -9.092 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21683 GHAHPCTNFIYDINLNPPPPP 21 SLAY-screened peptide P33 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCACGCCCACCCTTGCACGAACTTTATCTACGACATTAACCTGAACCCCCCCCCCCCCCCC GHAHPCTNFIYDINLNPPPPP -8.993 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21684 YASHCPCRTICYHVSP 16 SLAY-screened peptide P34 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGCCTCCCACTGCCCCTGTCGCACCATTTGTTATCACGTTTCGCCGTAGTACTCCAGGTAA YASHCPCRTICYHVSP*YSR* -8.968 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21685 SKCSITTRRQYAHPRSAV 18 SLAY-screened peptide P35 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCAAGTGCAGTATCACTACCAGGAGGCAGTACGCGCACCCTCGTAGTGCGGTTTAGAATTAA SKCSITTRRQYAHPRSAV*N* -8.946 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21686 TREATPCARIRSDSFGTT 18 SLAY-screened peptide P36 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCGTGAGGCGACTCCCTGCGCGCGTATTCGCTCCGACTCTTTTGGGACGACCTAGCCCTAA TREATPCARIRSDSFGTT*P* -8.937 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21687 TPMDRSLCHNHTL 13 SLAY-screened peptide P37 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCTATGGATCGTAGCCTCTGTCATAACCATACGCTTTAGATGGCGCAGGATAGTCATTAA TPMDRSLCHNHTL*MAQDSH* -8.916 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21688 SVRSSAPLMRVIGNCPSNHH 20 SLAY-screened peptide P38 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGTGCGTTCTAGCGCGCCTCTTATGCGTGTGATCGGCAATTGCCCGAGTAATCACCACTAA SVRSSAPLMRVIGNCPSNHH* -8.863 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21689 LLYEVDPAT 9 SLAY-screened peptide P39 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTTTACGAGGTCGACCCTGCCACTTAGTTCCATTCCCCCCCCCCCGCCCCCCCCCCCCCT LLYEVDPAT*FHSPPPAPPPP -8.836 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21690 SGSSPRNTQTP 11 SLAY-screened peptide P40 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGGGTCGTCGCCGCGGAATACGCAGACGCCCTAGGATTATTGCACTATGGTTCACACGTAA SGSSPRNTQTP*DYCTMVHT* -8.805 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21691 ANPAYKFKTCILCL 14 SLAY-screened peptide P41 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAATCCTGCTTATAAGTTTAAAACATGTATACTATGTCTGTGAGGGGGTTCGACAACTAAC ANPAYKFKTCILCL*GGSTTN -8.804 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21692 HTSNEDKTVYPVHSECIFDY 20 SLAY-screened peptide P42 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACCAGTAATGAGGATAAGACCGTGTATCCGGTTCACTCTGAGTGTATTTTTGACTATTAA HTSNEDKTVYPVHSECIFDY* -8.804 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21693 LYAEVGRLLIDLGAT 15 SLAY-screened peptide P43 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTACGCGGAGGTGGGGCGTCTCCTGATCGACCTTGGGGCCACCTAACTGAGTAAGTCGACC LYAEVGRLLIDLGAT*LSKST -8.765 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21694 RLDLASPFDIGIEGLSPANL 20 SLAY-screened peptide P44 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTCGATCTCGCTTCGCCTTTTGATATTGGTATTGAGGGTCTCTCCCCGGCTAACCTTTAA RLDLASPFDIGIEGLSPANL* -8.762 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21695 FYVPLRSSQPQPPISCRHTP 20 SLAY-screened peptide P45 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTACGTTCCCCTCCGGAGCTCCCAGCCTCAGCCCCCCATTTCCTGCCGCCACACCCCCTAA FYVPLRSSQPQPPISCRHTP* -8.749 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21696 LLVSSPSMRPIAVTPSGPAPN 21 SLAY-screened peptide P46 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTCGTCAGTTCTCCCTCCATGCGCCCGATAGCAGTAACCCCCTCGGGTCCTGCCCCTAAC LLVSSPSMRPIAVTPSGPAPN -8.745 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21697 THHMTGGVSWRGAPSLPYVN 20 SLAY-screened peptide P47 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCACCACATGACCGGCGGTGTCAGTTGGAGGGGGGCCCCCAGCCTCCCTTATGTCAATTAA THHMTGGVSWRGAPSLPYVN* -8.737 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21698 THHMTGGVSWRGAPSLPYVN 20 SLAY-screened peptide P48 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCATCACATGACCGGCGGTGTCAGTTGGAGGGGGGCCCCCAGCCTCCCTTATGTCAATTAA THHMTGGVSWRGAPSLPYVN* -8.737 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21699 AYRRLPLHARSPTVRVNLET 20 SLAY-screened peptide P49 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCTACCGCCGTTTGCCCCTTCATGCTCGTTCTCCCACTGTTCGCGTTAATCTGGAGACGTAA AYRRLPLHARSPTVRVNLET* -8.731 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21700 SPDFLRCSHTSRFVAYLLLS 20 SLAY-screened peptide P50 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCCGGATTTCCTGCGGTGCAGTCATACGTCTCGCTTTGTCGCCTATTTGTTGCTCTCGTAA SPDFLRCSHTSRFVAYLLLS* -8.723 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21701 EYPCILTQTAVNNSNSDTVY 20 SLAY-screened peptide P51 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGTACCCCTGCATTCTGACCCAGACTGCTGTTAACAACTCGAATTCCGATACGGTGTATTAA EYPCILTQTAVNNSNSDTVY* -8.716 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21702 RPSIAPRFSPIGSDNMLISF 20 SLAY-screened peptide P52 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCCGTCTATTGCCCCTCGTTTTAGTCCTATCGGTAGTGACAATATGCTCATTTCTTTTTAA RPSIAPRFSPIGSDNMLISF* -8.714 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21703 CTGYHKLNARDTVNSDISSS 20 SLAY-screened peptide P53 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTACCGGCTACCATAAGCTTAATGCCAGGGACACTGTTAATTCCGATATTTCGTCCAGTTAA CTGYHKLNARDTVNSDISSS* -8.684 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21704 IRVSNQSGLYGCPITLDWRL 20 SLAY-screened peptide P54 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCGTGTTTCTAATCAGTCCGGCCTTTACGGTTGTCCTATCACTCTCGATTGGCGCCTGTAA IRVSNQSGLYGCPITLDWRL* -8.667 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21705 DYRCGTRRFTIWAHLLGI 18 SLAY-screened peptide P55 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTACCGTTGTGGTACTCGTCGGTTTACGATTTGGGCTCATCTCTTGGGCATTTAGGTTTAA DYRCGTRRFTIWAHLLGI*V* -8.653 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21706 TGADGAHSCLITHYTENYGN 20 SLAY-screened peptide P56 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGGGCGGACGGCGCTCACAGCTGCTTGATTACGCACTATACTGAGAATTATGGCAATTAA TGADGAHSCLITHYTENYGN* -8.641 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21707 IVLGAIHHYSSPSALSRVLQ 20 SLAY-screened peptide P57 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGTCCTTGGCGCGATTCATCATTATTCTTCTCCTTCTGCTCTGTCTCGCGTCCTCCAGTAA IVLGAIHHYSSPSALSRVLQ* -8.636 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21708 ANLLIWLGLYLSHQNRRVDD 20 SLAY-screened peptide P58 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTAACCTCCTGATTTGGCTCGGGTTGTATCTTTCCCACCAGAATAGGCGGGTCGACGATTAA ANLLIWLGLYLSHQNRRVDD* -8.62 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21709 LDPSYIFLDSSPMLRAESIN 20 SLAY-screened peptide P59 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGGATCCTTCTTATATCTTTCTCGACTCGTCGCCGATGCTTCGGGCGGAGAGCATTAACTAA LDPSYIFLDSSPMLRAESIN* -8.59 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21710 GNHLACLGVRLIRGFNLHHL 20 SLAY-screened peptide P60 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCAATCATCTGGCTTGCTTGGGTGTTCGCCTTATTCGTGGCTTTAACCTGCATCATTTGTAA GNHLACLGVRLIRGFNLHHL* -8.552 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21711 HGVHHLNDHLSFLTLNLSLH 20 SLAY-screened peptide P61 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGGCGTTCATCACCTTAACGATCACTTGTCGTTTCTGACCCTTAATCTTTCCCTTCATTAA HGVHHLNDHLSFLTLNLSLH* -8.52 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21712 IRSCLRTVRLLVTTHYYHRE 20 SLAY-screened peptide P62 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCGTTCTTGTCTCCGTACGGTTCGTCTCCTTGTCACTACGCATTATTATCATCGCGAGTAA IRSCLRTVRLLVTTHYYHRE* -8.497 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21713 YDLDRGCAYNLLVYAERYYQ 20 SLAY-screened peptide P63 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGACTTGGATCGGGGTTGTGCTTATAATCTCCTTGTCTATGCGGAGCGTTACTATCAGTAA YDLDRGCAYNLLVYAERYYQ* -8.477 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21714 RNLHLTASPVRVPRHRPINS 20 SLAY-screened peptide P64 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAACCTTCATCTCACGGCGTCCCCGGTGCGTGTCCCGAGGCATCGTCCGATCAATAGTTAA RNLHLTASPVRVPRHRPINS* -8.421 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21715 RSSFHRIIYFIENHHIKNAI 20 SLAY-screened peptide P65 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTCTAGCTTTCATCGCATTATTTACTTCATTGAGAATCATCATATCAAGAACGCGATCTAA RSSFHRIIYFIENHHIKNAI* -8.419 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21716 QLTMNNPRMPSSA 13 SLAY-screened peptide P66 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCTTACTATGAACAACCCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACGCCATTTAA QLTMNNPRMPSSA*KKKNAI* -8.411 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21717 RCPHISASYVVLPGVIHSTT 20 SLAY-screened peptide P67 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTGCCCCCACATTAGTGCCAGCTATGTTGTTCTTCCCGGTGTTATCCATTCGACGACCTAA RCPHISASYVVLPGVIHSTT* -8.4 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21718 RRVRHRILSDIRVAHYRRWP 20 SLAY-screened peptide P68 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCGCGTCCGCCATCGTATCCTTAGTGACATCCGCGTGGCGCATTATAGGAGGTGGCCGTAA RRVRHRILSDIRVAHYRRWP* -8.381 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21719 TRTSSQTVAGNPRYNNSERS 20 SLAY-screened peptide P69 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCGCACTTCGTCTCAGACCGTTGCTGGTAATCCCAGGTACAATAATTCTGAGCGGTCCTAA TRTSSQTVAGNPRYNNSERS* -8.38 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21720 ALAYFHRVCA 10 SLAY-screened peptide P70 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCTGGCGTATTTCCATCGGGTTTGCGCTTAGGATCAGAGTGTCGTGTGCGTCACTTGGTAA ALAYFHRVCA*DQSVVCVTW* -8.365 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21721 SHNPHIRGPIQRSRKRPRRT 20 SLAY-screened peptide P71 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCATAACCCTCATATTCGGGGCCCCATCCAGAGGTCTCGTAAGCGTCCTAGGAGGACCTAA SHNPHIRGPIQRSRKRPRRT* -8.345 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21722 RSPCAPYAPPPLTFFRTVSA 20 SLAY-screened peptide P72 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAGTCCTTGCGCGCCGTACGCCCCCCCCCCTCTTACTTTCTTCCGTACCGTCAGTGCTTAA RSPCAPYAPPPLTFFRTVSA* -8.329 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21723 CTPAPPGIPCCSAYTFYYNR 20 SLAY-screened peptide P73 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTACCCCGGCGCCCCCTGGGATTCCTTGTTGTTCGGCTTACACTTTTTATTATAATCGCTAA CTPAPPGIPCCSAYTFYYNR* -8.325 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21724 STVQYHWNNSPFDSHARRTI 20 SLAY-screened peptide P74 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTACCGTCCAGTATCATTGGAATAATAGTCCTTTTGACAGTCATGCTCGCCGGACGATCTAA STVQYHWNNSPFDSHARRTI* -8.161 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21725 LTFHCHHNNDCNFNYLSSTL 20 SLAY-screened peptide P75 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACCTTTCATTGCCATCATAATAATGATTGTAATTTCAATTATCTGAGCAGTACTCTGTAA LTFHCHHNNDCNFNYLSSTL* -8.127 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21726 PKHSYSNVLA 10 SLAY-screened peptide P76 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAAGCATAGTTACAGTAACGTTTTGGCTTAGTATGACAACCTGGGTTACACTAGTAATTAA PKHSYSNVLA*YDNLGYTSN* -8.121 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21727 SSNYRQSECYDTSSFTYVLI 20 SLAY-screened peptide P77 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCTAATTATCGCCAGTCGGAGTGTTACGATACCTCTTCCTTTACGTACGTCCTTATTTAA SSNYRQSECYDTSSFTYVLI* -8.093 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21728 IGDVMATVATLINASSLYFP 20 SLAY-screened peptide P78 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGGCGACGTTATGGCTACTGTTGCTACTCTTATTAATGCTTCTAGCCTTTACTTTCCTTAA IGDVMATVATLINASSLYFP* -8.09 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21729 NVILRNSGLHASICSPPPPPP 21 SLAY-screened peptide P79 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGTCATCCTGCGCAACAGCGGGCTCCACGCTAGCATCTGTTCCCCCCCCCCCCCCCCCCCC NVILRNSGLHASICSPPPPPP -8.039 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21730 VASVFNCRNCLSYSNPNDTP 20 SLAY-screened peptide P80 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGCGTCTGTGTTCAATTGCCGTAATTGTCTTTCTTATTCGAATCCTAATGACACTCCTTAA VASVFNCRNCLSYSNPNDTP* -6.795 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21731 TASHSSSQYPKT 12 SLAY-screened peptide P81 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGCGAGTCATAGTTCGTCTCAGTATCCTAAGACGTAGGTCTAGACTCTGACTATTTCTTAA TASHSSSQYPKT*V*TLTIS* -6.774 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21732 LWNWDCFCFLRYHFGKRTTN 20 SLAY-screened peptide P82 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTGGAACTGGGATTGTTTCTGTTTCCTTCGTTATCACTTTGGGAAGCGTACCACTAATTAA LWNWDCFCFLRYHFGKRTTN* -6.704 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21733 PLLHIFNSTAMYIY 14 SLAY-screened peptide P83 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCCTGCATATTTTTAATTCTACCGCTATGTATATTTATTAGATCAACGCGCACAATTAA PLLHIFNSTAMYIY*INAHN* -6.629 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21734 PYGASTANIDFLDVFIYNTT 20 SLAY-screened peptide P84 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTACGGGGCGAGCACTGCGAATATTGATTTTCTGGATGTGTTTATCTACAATACGACGTAA PYGASTANIDFLDVFIYNTT* -6.558 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21735 SS 2 SLAY-screened peptide P85 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTCTTAGACTTTTGTTTCTATCTGCTCTACGATGTACTCCGACTTCTGCACTTATGCCTAA SS*TFVSICSTMYSDFCTYA* -6.536 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21736 TK 2 SLAY-screened peptide P86 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCAAGTAGCATAATAAGGCGGTCAATTATAAGCGTTCTGTGTCTATTGAGACTGATTTTTAA TK*HNKAVNYKRSVSIETDF* -6.471 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21737 STLCIQSRPSNTSCIHLAKN 20 SLAY-screened peptide P87 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGACGCTGTGTATTCAGTCTCGTCCTTCGAATACCTCCTGTATCCACCTTGCGAAGAACTAA STLCIQSRPSNTSCIHLAKN* -6.43 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21738 TIRLHVSIRIYLWRRRMVSA 20 SLAY-screened peptide P88 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTATCCGGTTGCATGTCAGCATCCGCATTTACCTTTGGAGGCGCCGCATGGTGTCTGCGTAA TIRLHVSIRIYLWRRRMVSA* -6.219 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21739 TQLYHTWH 8 SLAY-screened peptide P89 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCAGCTGTATCATACGTGGCACTAGACGAATAATGAGACTATTCCTAACTATAATGCGTAA TQLYHTWH*TNNETIPNYNA* -6.152 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21740 LPLKASQH 8 SLAY-screened peptide P90 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCGCTTAAGGCTAGCCAGCACTAGAACGTGTGTCGTACTCAGACTGGTAATAATGCTTAA LPLKASQH*NVCRTQTGNNA* -5.974 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21741 CALIIIFFYVRVCVRVSLTC 20 SLAY-screened peptide P91 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGCTTTGATCATTATTTTTTTCTACGTTCGGGTTTGCGTGCGTGTGAGTCTGACGTGCTAA CALIIIFFYVRVCVRVSLTC* -5.958 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21742 YGRSHATPNSDVSSMSPITA 20 SLAY-screened peptide P92 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGGTAGGTCTCATGCTACTCCTAATAGTGACGTGTCTAGCATGAGTCCGATCACTGCCTAA YGRSHATPNSDVSSMSPITA* -5.944 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21743 RLAHFPNHAVCDPHIINKPL 20 SLAY-screened peptide P93 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCTCGCTCACTTTCCGAATCATGCTGTCTGCGATCCTCATATTATCAATAAGCCGCTTTAA RLAHFPNHAVCDPHIINKPL* -5.777 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21744 RLGHDSNPWHIFRYNNNIPI 20 SLAY-screened peptide P94 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTTGGGTCATGATAGCAACCCTTGGCATATTTTCCGTTATAATAACAATATCCCCATTTAA RLGHDSNPWHIFRYNNNIPI* -5.759 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21745 RYHTHYC 7 SLAY-screened peptide P95 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTACCACACGCACTACTGCTAGGCTACGAATAATTATTTTAATGACGATTATTTTGCCTAA RYHTHYC*ATNNYFNDDYFA* -5.733 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21746 LQLSPRYVSRSYDCPTPLTT 20 SLAY-screened peptide P96 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCAGCTTAGCCCTCGTTATGTTTCGCGCAGTTATGATTGCCCTACTCCTCTCACTACTTAA LQLSPRYVSRSYDCPTPLTT* -5.69 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21747 RRCPPSSFAGHDPHRPIY 18 SLAY-screened peptide P97 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCGGTGCCCTCCTTCTTCGTTTGCTGGTCATGACCCTCATAGGCCTATTTATTAGATCTAA RRCPPSSFAGHDPHRPIY*I* -5.652 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21748 SSWAGHTRCGRCHPRYCYVT 20 SLAY-screened peptide P98 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTCTTGGGCTGGCCATACTCGCTGTGGCCGTTGCCATCCTAGGTACTGTTATGTCACTTAA SSWAGHTRCGRCHPRYCYVT* -5.625 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21749 LYCNHHTTLRCPKITVQNTR 20 SLAY-screened peptide P99 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTACTGTAATCATCACACTACGCTGCGTTGTCCTAAGATTACGGTCCAGAATACCAGGTAA LYCNHHTTLRCPKITVQNTR* -5.55 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21750 GHCSQIRFTACPIHALCNGT 20 SLAY-screened peptide P100 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCATTGTTCTCAGATTCGTTTTACGGCTTGCCCTATCCATGCGCTGTGCAACGGTACTTAA GHCSQIRFTACPIHALCNGT* -5.494 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21751 LYMFNSTMSNVAYEFI 16 SLAY-screened peptide P101 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTATATGTTCAATAGTACTATGTCTAATGTCGCTTATGAGTTCATCTAGCCGAAGCCTTAA LYMFNSTMSNVAYEFI*PKP* -5.475 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21752 YVTASNLYFVNCFTMFVMAK 20 SLAY-screened peptide P102 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGTTACTGCGTCCAATCTGTATTTTGTCAATTGCTTTACTATGTTCGTTATGGCTAAGTAA YVTASNLYFVNCFTMFVMAK* -5.442 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21753 RRDCNIESHYLRTPRS 16 SLAY-screened peptide P103 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCGTGATTGTAATATTGAGTCTCACTATCTGCGGACGCCTCGTTCGTAACTGAGTAAGTCG RRDCNIESHYLRTPRS*LSKS -5.421 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21754 GSLTSIDRCELDHVGYIHYK 20 SLAY-screened peptide P104 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCAGTCTTACTAGTATTGATCGGTGCGAGCTGGACCATGTTGGTTATATCCATTACAAGTAA GSLTSIDRCELDHVGYIHYK* -5.395 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21755 FVTQYSPFLGYFAPTRCSVP 20 SLAY-screened peptide P105 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGTCACTCAGTACAGCCCTTTTCTTGGCTATTTTGCTCCTACGCGTTGTTCCGTTCCGTAA FVTQYSPFLGYFAPTRCSVP* -5.357 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21756 IVFSGHDLQTDYLNNRIHLV 20 SLAY-screened peptide P106 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGTGTTTTCGGGGCACGATCTGCAGACTGACTATCTTAATAACAGGATCCACCTGGTGTAA IVFSGHDLQTDYLNNRIHLV* -5.343 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21757 PSFPSIYIRLSRIRHRHRRG 20 SLAY-screened peptide P107 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCCTTTCCGTCTATCTACATTCGTCTCTCCCGCATCCGTCACCGGCATCGTCGTGGCTAA PSFPSIYIRLSRIRHRHRRG* -5.325 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21758 ADSQHAPP 8 SLAY-screened peptide P108 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGACTCGCAGCACGCCCCTCCTTAGAATCACTATAAGTTTTATGATATTAGCGAGCCCTAA ADSQHAPP*NHYKFYDISEP* -5.287 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21759 YPYDRLSNVFDSLHYYCIQT 20 SLAY-screened peptide P109 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCCTTATGATCGCTTGTCTAACGTGTTCGATAGCCTTCATTATTATTGTATTCAGACCTAA YPYDRLSNVFDSLHYYCIQT* -5.205 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21760 PQLFTNHTPDSSYGIILAL 19 SLAY-screened peptide P110 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCAGCTGTTTACCAATCACACTCCTGATTCTAGCTATGGCATTATTCTTGCTTTGTAGTAA PQLFTNHTPDSSYGIILAL** -5.112 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21761 ERAPSYHTRSSSDSSNSGET 20 SLAY-screened peptide P111 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCGTGCTCCTAGTTATCATACTCGGAGCTCGAGTGACTCGAGCAATAGCGGTGAGACCTAA ERAPSYHTRSSSDSSNSGET* -5.096 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21762 TLVHNDSLSAQEPPPLSQ 18 SLAY-screened peptide P112 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTTGTGCATAATGATAGTTTGTCTGCTCAGGAGCCGCCGCCTCTGTCTCAGTAGGCTTAA TLVHNDSLSAQEPPPLSQ*A* -5.093 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21763 SHNCIHYP 8 SLAY-screened peptide P113 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCACAATTGTATTCACTATCCTTAGATGGATTTGTAGAACATGGCTCTGAAGAATGGGTAA SHNCIHYP*MDL*NMALKNG* -5.025 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21764 CPVQQSTYDKCSQPYRDTQH 20 SLAY-screened peptide P114 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCGGTTCAGCAGAGCACTTACGATAAGTGTTCTCAGCCTTACCGTGATACTCAGCATTAA CPVQQSTYDKCSQPYRDTQH* -4.993 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21765 RPYPPNFRRTPTQLPHLLVS 20 SLAY-screened peptide P115 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCCTATCCGCCGAACTTCAGGCGTACTCCCACCCAGCTTCCGCATCTTCTGGTCTCTTAA RPYPPNFRRTPTQLPHLLVS* -4.902 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21766 KRELT 5 SLAY-screened peptide P116 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCGCGAGTTGACTTAGATGCAGGCCCCTAAGCCTTTTATTTTTTTTGCGAATCACTGCTAA KRELT*MQAPKPFIFFANHC* -4.755 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21767 FDNTRMFCTIDIYNTDLHMH 20 SLAY-screened peptide P117 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTGATAACACCCGTATGTTCTGTACCATTGATATCTATAACACTGATTTGCATATGCATTAA FDNTRMFCTIDIYNTDLHMH* -4.737 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21768 DDPIVFVSRTNVLPHY 16 SLAY-screened peptide P118 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGACCCTATTGTTTTCGTCTCTCGTACGAATGTGTTGCCGCACTATTAGCATGCGGCGTAA DDPIVFVSRTNVLPHY*HAA* -4.637 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21769 TPNVYHNGDGRVPLHCSLSL 20 SLAY-screened peptide P119 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCCGAACGTTTATCACAATGGCGACGGTCGTGTGCCTCTTCATTGCAGTCTGTCGCTCTAA TPNVYHNGDGRVPLHCSLSL* -4.628 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21770 RFRGHHNVNSWFVIFSHHHD 20 SLAY-screened peptide P120 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTTTCGGGGTCACCATAATGTTAATTCTTGGTTTGTCATTTTTTCTCATCACCATGATTAA RFRGHHNVNSWFVIFSHHHD* -4.61 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21771 PT 2 SLAY-screened peptide P121 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACCTAGCTGGTTTGGAGTGAGTATTTGCGTCTCTATCACGTTATTCTTTTTGCTCTTTAA PT*LVWSEYLRLYHVILFAL* -4.604 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21772 RTEVLPYRNTQSGIPNYEFS 20 SLAY-screened peptide P122 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGACTGAGGTTCTCCCTTACCGTAATACGCAGTCTGGTATTCCGAATTATGAGTTTAGTTAA RTEVLPYRNTQSGIPNYEFS* -4.593 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21773 ADMLLHRSNSNEHDHCAILL 20 SLAY-screened peptide P123 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGACATGCTGTTGCATCGGTCGAACAGTAACGAGCATGATCATTGTGCGATTTTGCTCTAA ADMLLHRSNSNEHDHCAILL* -4.587 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21774 VPLAVASEPGPTLNGPPRAT 20 SLAY-screened peptide P124 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCCCCTCGCCGTCGCTAGCGAGCCTGGCCCCACTTTGAACGGCCCTCCCCGGGCCACTTAA VPLAVASEPGPTLNGPPRAT* -4.526 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21775 TFAITDMFSETNSITRFN 18 SLAY-screened peptide P125 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTTTGCTATTACTGACATGTTCTCGGAGACCAATAGTATTACTCGTTTTAACTAGCTCTAA TFAITDMFSETNSITRFN*L* -4.493 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21776 LLPPGDLYQNRHIFPECNHN 20 SLAY-screened peptide P126 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCTGCCTCCTGGCGATCTCTATCAGAATCGCCATATCTTCCCGGAGTGCAACCATAATTAA LLPPGDLYQNRHIFPECNHN* -4.489 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21777 PHGHSFHVYISLLFY 15 SLAY-screened peptide P127 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCACGGGCATAGCTTTCACGTCTATATTTCTCTTCTTTTTTACTAGGGGCTGGTGAATTAA PHGHSFHVYISLLFY*GLVN* -4.481 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21778 CRTTSNHPLEIRRYCMYHGR 20 SLAY-screened peptide P128 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGTACTACTAGTAATCATCCTCTGGAGATTCGGAGGTATTGCATGTACCACGGGAGGTAA CRTTSNHPLEIRRYCMYHGR* -4.468 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21779 THKFHHRGRGYHSPNACLAG 20 SLAY-screened peptide P129 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCATAAGTTCCACCATCGGGGTCGCGGTTATCATTCTCCTAATGCTTGTCTTGCCGGCTAA THKFHHRGRGYHSPNACLAG* -4.452 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21780 NDLYIGLYELMVNPARDHPN 20 SLAY-screened peptide P130 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGACCTGTATATTGGGCTGTACGAGCTTATGGTTAATCCTGCTAGGGATCATCCTAATTAA NDLYIGLYELMVNPARDHPN* -4.447 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21781 ANLLLTLFMLTLRVGLAILSN 21 SLAY-screened peptide P131 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAACTTGCTGCTAACCCTATTCATGCTAACCTTACGTGTCGGGCTTGCTATACTATCTAAC ANLLLTLFMLTLRVGLAILSN -4.402 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21782 NPFLGSGSIGLFHRSMCCIL 20 SLAY-screened peptide P132 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCCGTTTCTTGGTAGCGGCTCTATCGGCCTGTTTCACAGGTCGATGTGCTGTATTTTGTAA NPFLGSGSIGLFHRSMCCIL* -4.385 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21783 RLHFGRGARVHVHYGMGAVH 20 SLAY-screened peptide P133 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCTTCATTTCGGCCGTGGTGCGCGCGTGCATGTCCATTATGGGATGGGTGCGGTCCACTAA RLHFGRGARVHVHYGMGAVH* -4.384 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21784 RLASNHNPHHLHTSHQE 17 SLAY-screened peptide P134 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTTGGCCTCTAATCACAATCCGCATCATCTGCATACTAGTCATCAGGAGTAACTGAGTAAG RLASNHNPHHLHTSHQE*LSK -4.38 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21785 LVDGSWYSRPYVHSAGPPRV 20 SLAY-screened peptide P135 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGTCGATGGTAGTTGGTATTCTCGGCCCTACGTTCATAGCGCCGGTCCGCCCCGGGTTTAA LVDGSWYSRPYVHSAGPPRV* -4.376 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21786 LAGACPLHNSPNNGF 15 SLAY-screened peptide P136 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGCTGGTGCCTGTCCTCTGCATAACTCTCCTAATAATGGTTTCTAGGTTTAGATCGTCTAA LAGACPLHNSPNNGF*V*IV* -4.374 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21787 SPISNTA 7 SLAY-screened peptide P137 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCCTATTAGTAATACTGCCTAGACGCGTTTTCCTCATCGTGACTGTAAGTGTGCGAATTAA SPISNTA*TRFPHRDCKCAN* -4.371 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21788 SPIHAHCCTTNYHDIIVDFV 20 SLAY-screened peptide P138 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCCGATCCATGCTCACTGTTGCACGACTAACTACCACGATATTATTGTTGATTTTGTTTAA SPIHAHCCTTNYHDIIVDFV* -4.366 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21789 RWALEPHSIWFHLKKMHLT 19 SLAY-screened peptide P139 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTGGGCGCTGGAGCCCCACTCTATCTGGTTTCACCTGAAGAAGATGCACCTCACTTAGTAA RWALEPHSIWFHLKKMHLT** -4.364 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21790 TVPRSERCRYCQLTDYLFSC 20 SLAY-screened peptide P140 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTGCCTCGCTCTGAGCGCTGCCGGTACTGTCAGCTGACTGATTATTTGTTTTCGTGTTAA TVPRSERCRYCQLTDYLFSC* -4.358 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21791 PLG 3 SLAY-screened peptide P141 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTGGGTAGTATCTTGACAACCATTCTTATCGTTTCTATTGGTGCAAGACGACCCACTAA PLG*YLDNHSYRFYWCKTTH* -4.341 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21792 WSGVTHPNLLAILGIVCCLL 20 SLAY-screened peptide P142 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGTCTGGTGTCACTCACCCTAATCTTCTCGCTATCCTTGGTATTGTTTGTTGCCTGCTTTAA WSGVTHPNLLAILGIVCCLL* -4.332 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21793 PH 2 SLAY-screened peptide P143 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCATTAGACCATTACGTATAATGATAACAAGAGCCTTATTCCGGCTACTTTGAATTCGTAA PH*TITYNDNKSLIPATLNS* -4.319 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21794 LA 2 SLAY-screened peptide P144 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGCTTAGCCGATTCACCTCTTCAATACTAATCACCCTAATATTGACTATTTTTATCTCTAA LA*PIHLFNTNHPNIDYFYL* -4.318 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21795 AIEVHAAWMLVPC 13 SLAY-screened peptide P145 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGATTGAGGTGCATGCGGCGTGGATGCTCGTTCCGTGCTAGACGGCGAATACGGCCAACTAA AIEVHAAWMLVPC*TANTAN* -4.285 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21796 QLDNHHLLHLNLRYGCRAYL 20 SLAY-screened peptide P146 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCTGGACAATCACCATCTGCTTCATCTTAACCTGCGTTATGGTTGCCGTGCCTATTTGTAA QLDNHHLLHLNLRYGCRAYL* -4.245 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21797 HWYYVHFRDHSSLYTLLPDL 20 SLAY-screened peptide P147 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGGTACTATGTTCATTTCCGTGACCATTCGAGTCTCTATACCCTGCTTCCTGACCTTTAA HWYYVHFRDHSSLYTLLPDL* -4.236 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21798 RAAFNRLTRFCAYVYSWQ 18 SLAY-screened peptide P148 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGCTGCTTTTAATCGCCTTACTCGTTTTTGCGCCTATGTTTATTCTTGGCAGTAGTAGTAA RAAFNRLTRFCAYVYSWQ*** -4.223 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21799 SRFHPVVNAARPNAHEGYSA 20 SLAY-screened peptide P149 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCGGTTCCATCCTGTGGTTAACGCTGCTCGTCCTAATGCGCACGAGGGGTATAGCGCTTAA SRFHPVVNAARPNAHEGYSA* -4.211 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21800 LLTTPYSQLSNAVYLPCS 18 SLAY-screened peptide P150 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTCACTACTCCTTATTCCCAGTTGTCGAATGCGGTTTATTTGCCCTGCAGCTAGTTTTAA LLTTPYSQLSNAVYLPCS*F* -4.185 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21801 TPLRRHYSIRWLYVRIRRRN 20 SLAY-screened peptide P151 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCCCTTCGCCGGCATTACAGCATTCGTTGGCTGTACGTGCGTATTAGGCGTAGGAATTAA TPLRRHYSIRWLYVRIRRRN* -4.174 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21802 ATRRTATNLLGERTDAHTYR 20 SLAY-screened peptide P152 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTACTCGCCGGACTGCGACTAATCTTCTTGGTGAGCGTACTGATGCCCATACTTATAGGTAA ATRRTATNLLGERTDAHTYR* -4.169 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21803 AFTDDAVRIPGRRCTTFNCS 20 SLAY-screened peptide P153 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTTTTACTGACGATGCTGTTCGGATTCCGGGTAGGCGTTGTACTACGTTCAACTGCAGCTAA AFTDDAVRIPGRRCTTFNCS* -4.164 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21804 TFPPVHLSSDAILGDLHHAG 20 SLAY-screened peptide P154 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTTTCCGCCGGTGCATCTGTCTTCTGACGCGATTTTGGGCGATCTTCACCATGCTGGTTAA TFPPVHLSSDAILGDLHHAG* -4.162 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21805 AMQIPNSLCAISS 13 SLAY-screened peptide P155 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTATGCAGATTCCCAACTCTCTTTGCGCTATTAGTTCTTAGAATAACCCTTATGGTCTTTAA AMQIPNSLCAISS*NNPYGL* -4.146 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21806 NVSLDNHGMLPGMLKSFYC 19 SLAY-screened peptide P156 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGTCTCCCTCGACAATCACGGTATGCTTCCTGGTATGCTTAAGAGCTTTTATTGCTAGTAA NVSLDNHGMLPGMLKSFYC** -4.126 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21807 AGGYRHYMYGPHDWRFHRFY 20 SLAY-screened peptide P157 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGGCGGCTATCGCCACTATATGTATGGTCCTCATGATTGGCGCTTTCATCGGTTTTATTAA AGGYRHYMYGPHDWRFHRFY* -4.125 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21808 MYNSASDETTSSHSNTGNYN 20 SLAY-screened peptide P158 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTATAATAGCGCCTCTGATGAGACTACCTCCTCTCATAGTAATACTGGTAATTATAATTAA MYNSASDETTSSHSNTGNYN* -4.12 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21809 CPFTVSDTSASYRSTRSFYS 20 SLAY-screened peptide P159 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCTTTCACTGTGTCTGATACCTCTGCGTCTTATCGTTCTACTCGTTCGTTTTATTCTTAA CPFTVSDTSASYRSTRSFYS* -4.113 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21810 LATSTLDYHSHLYSGPNSYG 20 SLAY-screened peptide P160 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGCGACGTCCACGCTGGACTATCACAGTCACTTGTACAGCGGGCCTAACAGCTACGGTTAA LATSTLDYHSHLYSGPNSYG* -4.093 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21811 THDLAHNNNYFRVGSYLRLY 20 SLAY-screened peptide P161 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCACGACTTGGCGCACAACAACAATTATTTTCGCGTCGGTAGTTATCTTCGTCTTTATTAA THDLAHNNNYFRVGSYLRLY* -4.084 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21812 MALWNPLLCKANHDLYLDAN 20 SLAY-screened peptide P162 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGGCCTTGTGGAACCCTCTGCTGTGCAAGGCTAACCATGATCTGTATTTGGATGCTAACTAA MALWNPLLCKANHDLYLDAN* -4.062 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21813 KGVPVHIMPGAFFPSLVAGR 20 SLAY-screened peptide P163 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGGGTGTTCCTGTTCACATCATGCCTGGTGCTTTTTTCCCTTCTCTGGTTGCGGGCCGGTAA KGVPVHIMPGAFFPSLVAGR* -4.061 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21814 RLVSAEQHHNNSSYLAFMNE 20 SLAY-screened peptide P164 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCTTGTGTCTGCCGAGCAGCATCACAATAATAGTAGTTATCTGGCCTTTATGAATGAGTAA RLVSAEQHHNNSSYLAFMNE* -4.057 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21815 LVCLCDCFQPDRTGSSVSED 20 SLAY-screened peptide P165 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGTCTGCTTGTGTGATTGTTTCCAGCCCGATCGCACTGGTTCTAGTGTCTCTGAGGATTAA LVCLCDCFQPDRTGSSVSED* -4.035 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21816 TSNSPKALGNTASMSPMCHI 20 SLAY-screened peptide P166 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTCTAATTCTCCTAAGGCTCTGGGGAATACGGCCTCGATGAGCCCGATGTGCCATATTTAA TSNSPKALGNTASMSPMCHI* -4.022 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21817 NCAFERPNHPSPYYDFEYTI 20 SLAY-screened peptide P167 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTGCGCGTTTGAGCGTCCTAACCACCCTTCTCCGTATTATGACTTTGAGTATACTATTTAA NCAFERPNHPSPYYDFEYTI* -4.017 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21818 EPNHHSAVTGNRNNSATDND 20 SLAY-screened peptide P168 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCCCAATCATCACTCTGCTGTTACGGGTAATCGTAACAATAGCGCGACTGATAATGATTAA EPNHHSAVTGNRNNSATDND* -3.984 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21819 PDSPIVVVAQHKRPCDTLPF 20 SLAY-screened peptide P169 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGATAGTCCGATTGTGGTTGTTGCTCAGCATAAGCGTCCCTGTGATACGCTTCCTTTCTAA PDSPIVVVAQHKRPCDTLPF* -3.959 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21820 GHSPSLHCTMVIVIDGDNVT 20 SLAY-screened peptide P170 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCATTCTCCTTCTTTGCATTGTACGATGGTGATCGTTATTGATGGCGACAATGTCACTTAA GHSPSLHCTMVIVIDGDNVT* -3.949 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21821 PTRTEQWSTSNDSERTCLIL 20 SLAY-screened peptide P171 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACCCGGACGGAGCAGTGGAGTACTTCCAACGATAGTGAGCGGACGTGCCTCATTTTGTAA PTRTEQWSTSNDSERTCLIL* -3.936 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21822 CGHCHTCTIPYCGNLIVAIHY 21 SLAY-screened peptide P172 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGGCCATTGCCACACCTGCACCATTCCTTATTGCGGTAATCTTATTGTTGCTATCCACTAC CGHCHTCTIPYCGNLIVAIHY -3.921 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21823 PADTFFLLP 9 SLAY-screened peptide P173 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGCTGATACTTTCTTTCTGCTTCCGTAGCCTCGCATTGATTCCACCCACCGTCAGGCGTAA PADTFFLLP*PRIDSTHRQA* -3.919 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21824 TVTSETLYFRLTCYTSRP 18 SLAY-screened peptide P174 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTGACGAGTGAGACCCTTTACTTCAGGCTGACTTGCTATACGTCGCGTCCTTAGCCTTAA TVTSETLYFRLTCYTSRP*P* -3.91 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21825 PRSTDVARRPGLSTV 15 SLAY-screened peptide P175 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGGAGTACTGATGTGGCGCGGCGCCCTGGCCTCAGTACTGTTTAGACGCTTCAGACGTAA PRSTDVARRPGLSTV*TLQT* -3.901 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21826 TSPATYRHTNWRGAPPLPNT 20 SLAY-screened peptide P176 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTCTCCGGCTACTTACAGGCATACCAACTGGCGTGGTGCGCCGCCGCTCCCGAACACCTAA TSPATYRHTNWRGAPPLPNT* -3.884 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21827 SHLDNCTSVYNAANYTLMIG 20 SLAY-screened peptide P177 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCATCTCGATAATTGTACTAGTGTTTATAATGCCGCTAACTATACTTTGATGATTGGCTAA SHLDNCTSVYNAANYTLMIG* -3.882 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21828 ISSLR 5 SLAY-screened peptide P178 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTCGAGCTTGCGTTAGACTGAGATTCCTCCTGCGTGTGGCCACACTATTTCGTCGATGTAA ISSLR*TEIPPACGHTISSM* -3.88 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21829 LMYNATYDH 9 SLAY-screened peptide P179 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTATGTACAATGCTACTTATGATCACTAGCCTAATACCAACCTTACCAATGCCATGAATTAA LMYNATYDH*PNTNLTNAMN* -3.873 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21830 TLHAGLYSVIIMFYGRWVSN 20 SLAY-screened peptide P180 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTGCATGCCGGCCTGTATTCTGTTATTATTATGTTTTACGGTCGTTGGGTTTCTAATTAA TLHAGLYSVIIMFYGRWVSN* -3.868 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21831 PAEWSTVVGNFTYHFNYNLL 20 SLAY-screened peptide P181 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCGGAGTGGTCGACTGTTGTGGGTAATTTTACGTACCATTTCAATTATAATCTCTTGTAA PAEWSTVVGNFTYHFNYNLL* -3.866 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21832 TLYIITYWDPDYKNIVSLTI 20 SLAY-screened peptide P182 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTTGTACATTATTACGTATTGGGATCCTGATTACAAGAATATTGTTTCGCTTACGATTTAA TLYIITYWDPDYKNIVSLTI* -3.866 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21833 HPITIPNLLLAYRMPVLMLF 20 SLAY-screened peptide P183 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCCATTACTATTCCTAATCTTCTTTTGGCTTACCGCATGCCTGTTCTCATGCTGTTTTAA HPITIPNLLLAYRMPVLMLF* -3.852 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21834 GDCTHKYADLPNAISNLFLR 20 SLAY-screened peptide P184 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTGATTGCACTCACAAGTATGCTGATCTCCCTAATGCTATCAGCAACCTTTTTTTGCGCTAA GDCTHKYADLPNAISNLFLR* -3.849 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21835 GHVAMDPNINAVFHTTADTS 20 SLAY-screened peptide P185 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCACGTGGCCATGGATCCCAACATCAATGCTGTCTTTCACACTACTGCTGATACTTCTTAA GHVAMDPNINAVFHTTADTS* -3.846 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21836 IKTPKLDPN 9 SLAY-screened peptide P186 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAAGACGCCTAAGCTTGATCCTAATTAGTGCATTTGCAAGATTCCTGTGCTTTACCGTTAA IKTPKLDPN*CICKIPVLYR* -3.846 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21837 HNFPCVYLARRRSSTRRGRVT 21 SLAY-screened peptide P187 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAACTTTCCGTGTGTCTACCTTGCCAGGCGCCGTTCATCAACTCGCCGAGGTCGAGTAACT HNFPCVYLARRRSSTRRGRVT -3.838 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21838 PSHGLDSSRRTHCNYIRTCE 20 SLAY-screened peptide P188 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGCCATGGTCTTGATTCTTCGCGTCGCACGCATTGCAACTATATTCGCACTTGTGAGTAA PSHGLDSSRRTHCNYIRTCE* -3.836 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21839 SVRGRCCSGEYARSRAVGTP 20 SLAY-screened peptide P189 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGTTCGTGGTCGGTGTTGTAGCGGTGAGTATGCGCGGTCTCGGGCTGTGGGGACCCCCTAA SVRGRCCSGEYARSRAVGTP* -3.831 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21840 PLQSPALATVMRIDHPTPTV 20 SLAY-screened peptide P190 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTGCAGAGTCCCGCTTTGGCGACGGTTATGCGTATCGACCACCCGACTCCGACCGTTTAA PLQSPALATVMRIDHPTPTV* -3.824 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21841 NICDTYILRDNRPFLT 16 SLAY-screened peptide P191 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATATTTGTGATACTTACATTCTGCGCGACAATCGTCCTTTCTTGACGTAGATCAGCATTAAC NICDTYILRDNRPFLT*ISIN -3.808 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21842 WPPPNYQRHDALKEEETSNL 20 SLAY-screened peptide P192 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCCCCCGCCGAATTACCAGCGCCACGATGCTCTCAAGGAGGAGGAGACGTCCAATTTGTAA WPPPNYQRHDALKEEETSNL* -3.803 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21843 VLLQY 5 SLAY-screened peptide P193 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCTTTTGCAGTACTAGCCTAAAATTAGGGCTGGAGCAGCTACGGTTACAACACTACTTAAC VLLQY*PKIRAGAATVTTLLN -3.795 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21844 PPGPANHAHHICIWPSEPAH 20 SLAY-screened peptide P194 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCGGGCCCTGCTAATCACGCTCACCATATTTGCATTTGGCCCTCGGAGCCCGCCCATTAA PPGPANHAHHICIWPSEPAH* -3.762 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21845 HVIASGCAVLLNYFRVMLPS 20 SLAY-screened peptide P195 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGTTATCGCCAGCGGTTGCGCTGTTTTGCTTAACTATTTTAGGGTTATGCTTCCTTCCTAA HVIASGCAVLLNYFRVMLPS* -3.755 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21846 DHHRFIAPDISLARYFILYT 20 SLAY-screened peptide P196 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCATCACCGTTTCATTGCCCCTGATATTTCTCTTGCTAGGTACTTTATTCTGTATACGTAA DHHRFIAPDISLARYFILYT* -3.753 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21847 PSSSQVPGDHFHFSNYVTFLY 21 SLAY-screened peptide P197 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTTCCTCCCAGGTTCCCGGTGATCACTTTCACTTTAGCAATTATGTTACCTTCTTGTAC PSSSQVPGDHFHFSNYVTFLY -3.753 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21848 LFNLMSILNPDFSYYTNASN 20 SLAY-screened peptide P198 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTTTAACCTTATGTCTATTCTTAATCCTGACTTTAGCTACTATACTAACGCTTCTAATTAA LFNLMSILNPDFSYYTNASN* -3.748 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21849 STYF 4 SLAY-screened peptide P199 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTACTTACTTTTAGCTCTGTCACTATCTTCGTAACATTTCTCGGCAGAAGGGTGAGGCCTAA STYF*LCHYLRNISRQKGEA* -3.733 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21850 SNSCLSSPCNIHYSVIPDRN 20 SLAY-screened peptide P200 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGAATTCTTGCCTTTCTAGTCCTTGTAACATTCATTATAGTGTCATCCCTGATAGGAACTAA SNSCLSSPCNIHYSVIPDRN* -3.722 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21851 MQARPHWFSHDL 12 SLAY-screened peptide P201 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCAGGCTCGCCCCCACTGGTTCTCGCATGACCTGTAGTAGTGTAACTTGGATTATTATTAA MQARPHWFSHDL**CNLDYY* -3.697 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21852 NAPIISVCYCSTQILCLGDI 20 SLAY-screened peptide P202 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGCGCCTATTATCAGTGTCTGTTATTGCAGTACTCAGATTCTCTGCCTTGGTGATATTTAA NAPIISVCYCSTQILCLGDI* -3.692 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21853 NRPNM 5 SLAY-screened peptide P203 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCGTCCGAACATGTAGAAGTGCGGCACTTTGCCGCCTCGTTTTTAGGTTGCTATTGTCTAA NRPNM*KCGTLPPRF*VAIV* -3.684 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21854 PMHYLGSTTLKKNHLYHDSIN 21 SLAY-screened peptide P204 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATGCATTACCTCGGTTCTACTACTCTTAAGAAGAATCATCTGTACCATGACTCGATTAAC PMHYLGSTTLKKNHLYHDSIN -3.683 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21855 ASDSPFQECDHLFYISNYIL 20 SLAY-screened peptide P205 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGAGTGATTCTCCTTTCCAGGAGTGTGACCATCTCTTTTACATTTCTAATTATATCCTGTAA ASDSPFQECDHLFYISNYIL* -3.682 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21856 RPFSKHSYNTDNTDYYHSNC 20 SLAY-screened peptide P206 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCCCTTTTCTAAGCACTCCTACAACACGGATAATACTGATTATTATCATTCTAACTGCTAA RPFSKHSYNTDNTDYYHSNC* -3.675 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21857 LNMLLYSTFRFTCSGNDHYH 20 SLAY-screened peptide P207 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAACATGCTTCTTTACTCGACTTTTAGGTTTACGTGTTCGGGTAACGATCACTATCACTAA LNMLLYSTFRFTCSGNDHYH* -3.673 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21858 LLVSYCNGDIKHCHPNNSFS 20 SLAY-screened peptide P208 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTCGTGTCTTATTGCAATGGTGACATTAAGCATTGCCACCCTAATAATTCGTTTTCTTAA LLVSYCNGDIKHCHPNNSFS* -3.656 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21859 PLCYTLHSHNYNAAYYSSLS 20 SLAY-screened peptide P209 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTTTGTTACACGCTGCACTCCCATAATTATAATGCTGCTTATTATTCGAGTCTGTCGTAA PLCYTLHSHNYNAAYYSSLS* -3.656 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21860 PKTPASYCTIIVMVDNTVSL 20 SLAY-screened peptide P210 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAAGACGCCCGCGTCTTACTGTACGATTATCGTTATGGTTGACAATACTGTTTCTCTGTAA PKTPASYCTIIVMVDNTVSL* -3.653 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21861 LFHSEFSDTQRSIHNISDYL 20 SLAY-screened peptide P211 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTTTCATTCTGAGTTTAGTGATACTCAGAGGTCCATTCACAACATCAGCGATTACTTGTAA LFHSEFSDTQRSIHNISDYL* -3.648 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21862 WTSRFALLTKHFAIFVTILTN 21 SLAY-screened peptide P212 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGACTTCCCGCTTTGCGCTCCTTACTAAGCACTTCGCTATCTTTGTAACCATCCTAACTAAC WTSRFALLTKHFAIFVTILTN -3.633 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21863 HHRNLQLYTFLSLLWTHYAA 20 SLAY-screened peptide P213 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCACCGTAATCTGCAGTTGTATACGTTCCTGTCTTTGCTGTGGACTCATTACGCCGCGTAA HHRNLQLYTFLSLLWTHYAA* -3.633 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21864 PYARSLGTGGNYIVNIIPRY 20 SLAY-screened peptide P214 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTATGCCCGGAGCCTTGGCACTGGGGGTAATTATATTGTTAACATTATTCCTCGTTATTAA PYARSLGTGGNYIVNIIPRY* -3.631 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21865 SSNRHLGGSCQSPESDNYSIY 21 SLAY-screened peptide P215 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTCTAACCGTCACTTGGGTGGTAGCTGTCAGAGTCCCGAGAGCGATAACTATAGTATTTAC SSNRHLGGSCQSPESDNYSIY -3.628 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21866 SDD 3 SLAY-screened peptide P216 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGACGATTAGTATACGTGTATGCCGATTCCCACGTATTTCCGTGTCAATCTCTTCCCCTAA SDD*YTCMPIPTYFRVNLFP* -3.623 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21867 PPCCVTPPSILSFAVATCAT 20 SLAY-screened peptide P217 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTTGCTGCGTTACCCCGCCTAGCATCCTTAGTTTTGCTGTGGCTACGTGCGCGACGTAA PPCCVTPPSILSFAVATCAT* -3.612 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21868 ILVVSL 6 SLAY-screened peptide P218 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCTCGTCGTCTCGTTGTAGCATCCTGGCGCCTATCCCTCCATGCTGTGGTCTACCACTTAA ILVVSL*HPGAYPSMLWSTT* -3.596 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21869 PNLHSGNRPLYNLFAYAAHG 20 SLAY-screened peptide P219 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAATCTTCACTCTGGTAATAGGCCGCTCTATAATCTGTTTGCCTACGCTGCCCATGGTTAA PNLHSGNRPLYNLFAYAAHG* -3.591 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21870 HYFYLDVLAILPLHFKSIPC 20 SLAY-screened peptide P220 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTACTTCTATCTGGACGTGCTTGCTATTCTCCCGCTTCATTTTAAGAGTATTCCTTGTTAA HYFYLDVLAILPLHFKSIPC* -3.587 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21871 RDIITIRHCAYRHTPNTRIC 20 SLAY-screened peptide P221 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGATATTATCACGATCAGGCACTGTGCGTATCGCCATACGCCTAACACTCGCATTTGCTAA RDIITIRHCAYRHTPNTRIC* -3.579 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21872 RHIAYHCNMLFSDFLDRFLE 20 SLAY-screened peptide P222 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCATATTGCTTATCATTGCAACATGCTGTTTTCTGACTTTCTCGATCGTTTTCTCGAGTAA RHIAYHCNMLFSDFLDRFLE* -3.576 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21873 YSGTYTGFSNYCIVDCTI 18 SLAY-screened peptide P223 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAGTGGCACGTATACTGGTTTCTCTAATTATTGCATTGTGGATTGTACCATCTAGTATTAA YSGTYTGFSNYCIVDCTI*Y* -3.574 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21874 PPYNLHTDN 9 SLAY-screened peptide P224 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCTTATAACCTTCACACTGATAATTAGTCTTTTCGGGACGAGTATCTTAAGTCTAGGTAA PPYNLHTDN*SFRDEYLKSR* -3.573 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21875 AWNYNYGKPPLGINLQYLRT 20 SLAY-screened peptide P225 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTTGGAATTACAATTACGGCAAGCCTCCTCTGGGTATCAACCTGCAGTATCTCCGGACCTAA AWNYNYGKPPLGINLQYLRT* -3.572 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21876 RPFHTTPNFSRCLYPRDSFL 20 SLAY-screened peptide P226 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCTTTTCATACTACGCCTAACTTTAGCCGTTGTCTGTATCCGCGTGATTCTTTTCTCTAA RPFHTTPNFSRCLYPRDSFL* -3.571 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21877 PFGKHRSGLFPRHNSKTAQL 20 SLAY-screened peptide P227 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTTGGGAAGCATCGTTCTGGTCTTTTTCCTAGGCATAACAGCAAGACCGCGCAGCTGTAA PFGKHRSGLFPRHNSKTAQL* -3.569 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21878 PGGCPSLRMHDLDDTMHVLQ 20 SLAY-screened peptide P228 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGTGGCTGTCCTAGTCTTCGCATGCACGATCTCGATGATACTATGCACGTTCTTCAGTAA PGGCPSLRMHDLDDTMHVLQ* -3.565 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21879 RGSKDCAYPASSNLDSIILN 20 SLAY-screened peptide P229 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGGTTCGAAGGACTGTGCTTACCCTGCTTCTTCTAATTTGGATTCCATTATTCTGAACTAA RGSKDCAYPASSNLDSIILN* -3.556 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21880 PILCDLGVAYAIPPFCDD 18 SLAY-screened peptide P230 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGATCCTTTGTGACCTGGGTGTTGCTTATGCGATTCCGCCTTTCTGTGATGATTAGACCTAA PILCDLGVAYAIPPFCDD*T* -3.553 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21881 RRARGVYTWYSNLPSAQRVP 20 SLAY-screened peptide P231 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCGCGCTCGGGGCGTGTATACCTGGTATTCTAACCTTCCGTCGGCCCAGCGGGTTCCCTAA RRARGVYTWYSNLPSAQRVP* -3.543 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21882 RTLTFMVRIGAKMLFFEIRY 20 SLAY-screened peptide P232 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACCCTCACCTTTATGGTTCGCATTGGGGCCAAGATGCTCTTTTTTGAGATTAGGTATTAA RTLTFMVRIGAKMLFFEIRY* -3.537 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21883 RMGSSYTSGIDLWLVLHHNN 20 SLAY-screened peptide P233 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGATGGGGTCTTCCTACACTTCTGGTATTGACCTGTGGCTGGTGCTGCATCATAATAATTAA RMGSSYTSGIDLWLVLHHNN* -3.535 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21884 PYWLGTLDRVNYLGPTGYAF 20 SLAY-screened peptide P234 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTATTGGCTGGGTACTCTTGATCGCGTCAATTACCTTGGCCCCACGGGGTATGCCTTCTAA PYWLGTLDRVNYLGPTGYAF* -3.53 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21885 PGPYSKSLLSIRCADPN 17 SLAY-screened peptide P235 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGGCCCTTACTCTAAGTCTCTGCTTTCTATTCGGTGTGCTGACCCTAACTAGAACGCTTAA PGPYSKSLLSIRCADPN*NA* -3.523 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21886 YRNTTTIR 8 SLAY-screened peptide P236 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCGTAACACTACGACTATTCGTTAGGATCTCCATAGCAACGGCGACTCTAGTAGCCCTTAA YRNTTTIR*DLHSNGDSSSP* -3.521 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21887 SQTYCIWLRVRIRIAIIIRLN 21 SLAY-screened peptide P237 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCAGACTTACTGCATTTGGTTACGCGTACGAATCCGCATAGCAATAATTATACGACTTAAC SQTYCIWLRVRIRIAIIIRLN -3.517 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21888 TNFHMLNYYASPGCSYKEPL 20 SLAY-screened peptide P238 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAATTTCCATATGCTTAATTATTACGCCAGCCCGGGCTGCTCTTATAAGGAGCCCCTCTAA TNFHMLNYYASPGCSYKEPL* -3.514 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21889 RSNNVFTLPQNLHSANKLCP 20 SLAY-screened peptide P239 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTCGAATAATGTTTTTACGCTTCCTCAGAATCTTCATTCGGCTAATAAGCTCTGCCCTTAA RSNNVFTLPQNLHSANKLCP* -3.511 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21890 VGTSSLNGDKVPNLPRRVIR 20 SLAY-screened peptide P240 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGGGACGTCTAGCCTTAATGGCGACAAGGTTCCTAATTTGCCTAGGAGGGTCATTCGTTAA VGTSSLNGDKVPNLPRRVIR* -3.506 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21891 VSVILRPNGLNLSVRLSYAC 20 SLAY-screened peptide P241 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTCTGTTATTCTTAGGCCTAACGGTCTTAACCTTTCTGTTCGTCTGAGCTACGCTTGCTAA VSVILRPNGLNLSVRLSYAC* -3.504 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21892 PPGGYHCDLYFLILRH 16 SLAY-screened peptide P242 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCGGGCGGGTATCATTGTGATTTGTATTTTCTTATTCTTCGTCACTAGCAGAAGTAGTAA PPGGYHCDLYFLILRH*QK** -3.497 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21893 RLALTFIHRLYHPNHLNFHS 20 SLAY-screened peptide P243 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTGGCGCTCACGTTCATCCACAGGCTTTATCATCCTAATCACCTTAACTTTCACTCTTAA RLALTFIHRLYHPNHLNFHS* -3.489 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21894 IRYGPPCSHNLREHLPKTLE 20 SLAY-screened peptide P244 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAGGTATGGCCCGCCTTGTAGCCACAATCTTCGGGAGCATCTTCCGAAGACCCTCGAGTAA IRYGPPCSHNLREHLPKTLE* -3.478 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21895 GNLNFPIEWKARRMVEVKSQ 20 SLAY-screened peptide P245 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGAATCTTAATTTTCCTATTGAGTGGAAGGCGCGTCGTATGGTTGAGGTCAAGTCCCAGTAA GNLNFPIEWKARRMVEVKSQ* -3.477 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21896 DIHAITRVPDTQLIHFVCIS 20 SLAY-screened peptide P246 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATATTCATGCGATCACTCGTGTCCCTGATACGCAGCTTATCCATTTTGTCTGCATTTCTTAA DIHAITRVPDTQLIHFVCIS* -3.471 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21897 QSTSNLHMSYTVNGTNVLGR 20 SLAY-screened peptide P247 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTCCACTTCGAATCTCCATATGTCTTACACTGTCAACGGGACTAATGTCCTGGGGCGTTAA QSTSNLHMSYTVNGTNVLGR* -3.469 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21898 NSVDPIDSDIDMTYNALHSDY 21 SLAY-screened peptide P248 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTCGGTTGATCCTATTGATTCTGACATTGATATGACTTATAATGCCTTGCATAGTGATTAC NSVDPIDSDIDMTYNALHSDY -3.465 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21899 PPVRKRITVSYHILFNKNND 20 SLAY-screened peptide P249 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCCGTCCGTAAGCGTATCACCGTTAGTTATCATATTTTGTTTAATAAGAATAACGACTAA PPVRKRITVSYHILFNKNND* -3.454 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21900 PSDLVPTLSPNNRGPPEYSP 20 SLAY-screened peptide P250 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTCGGACCTGGTTCCCACTCTTTCTCCGAACAACCGCGGCCCGCCGGAGTACTCTCCCTAA PSDLVPTLSPNNRGPPEYSP* -3.453 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21901 TCMHNNWLPLATLSDRRHLF 20 SLAY-screened peptide P251 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGTATGCATAATAATTGGCTTCCGCTTGCGACCCTGAGCGACAGGAGGCATTTGTTTTAA TCMHNNWLPLATLSDRRHLF* -3.452 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21902 TGRGRHPHGTRTVRIATPNN 20 SLAY-screened peptide P252 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGCCGGGGTAGGCACCCTCACGGCACTCGTACGGTTCGTATTGCTACGCCGAACAATTAA TGRGRHPHGTRTVRIATPNN* -3.45 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21903 STPGLCTTASPPFVP 15 SLAY-screened peptide P253 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGACCCCCGGTCTTTGCACCACCGCGAGTCCTCCTTTCGTGCCGTAGACGATCTACTACTAA STPGLCTTASPPFVP*TIYY* -3.449 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21904 CPPLLGYSARDRLSIYGSIV 20 SLAY-screened peptide P254 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCTCCTCTCCTGGGGTACTCCGCTAGGGACCGTCTCAGTATTTATGGTTCGATTGTGTAA CPPLLGYSARDRLSIYGSIV* -3.447 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21905 SSYNAHMM 8 SLAY-screened peptide P255 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCAGTTACAATGCTCATATGATGTAGGGTTGGCATAGCACCATCAATACTTTTAAGTGTTAA SSYNAHMM*GWHSTINTFKC* -3.447 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21906 ANSLCFIRGPPSFISKLHNI 20 SLAY-screened peptide P256 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAACTCGCTCTGTTTTATCCGGGGTCCGCCGTCCTTTATCAGCAAGCTTCATAATATTTAA ANSLCFIRGPPSFISKLHNI* -3.446 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21907 KCCSPDTCPTVPEIHMPLSS 20 SLAY-screened peptide P257 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGTGCTGTAGTCCTGACACTTGCCCTACGGTTCCTGAGATTCATATGCCTCTCTCGAGTTAA KCCSPDTCPTVPEIHMPLSS* -3.444 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21908 HYHRFATGATRSSYHTHAFI 20 SLAY-screened peptide P258 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTACCATCGCTTCGCCACGGGTGCTACGCGCAGCTCTTACCATACTCATGCGTTTATTTAA HYHRFATGATRSSYHTHAFI* -3.444 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21909 TSVDPNICICILFGHLSGYY 20 SLAY-screened peptide P259 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTCCGTGGATCCTAACATTTGTATCTGTATCCTTTTTGGTCATCTCAGTGGTTACTATTAA TSVDPNICICILFGHLSGYY* -3.443 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21910 CPTSALPSSGLLTVPTYASS 20 SLAY-screened peptide P260 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCCACCTCGGCGCTCCCGTCCTCGGGGCTTCTCACTGTGCCTACTTACGCGTCGAGTTAA CPTSALPSSGLLTVPTYASS* -3.44 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21911 TYTQ 4 SLAY-screened peptide P261 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTACACGCAGTAGAAGCATTGGCAGGACCCGCACGCGGCTACGACTTCGTCCGAGAATTAA TYTQ*KHWQDPHAATTSSEN* -3.423 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21912 DTLFHPKLHPHSAPTCTM 18 SLAY-screened peptide P262 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATACCTTGTTTCACCCCAAGCTGCATCCCCATTCCGCCCCTACTTGCACCATGTAGTTCTAA DTLFHPKLHPHSAPTCTM*F* -3.421 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21913 YPIRHSLPYAPYMFRTVACP 20 SLAY-screened peptide P263 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCCTATTAGGCATTCTCTTCCTTACGCTCCGTATATGTTCCGCACTGTCGCTTGCCCGTAA YPIRHSLPYAPYMFRTVACP* -3.421 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21914 PC 2 SLAY-screened peptide P264 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCTAGGGTGACGGGTCGAGTATTAACAGGTATAGCGCCCCGGCGTAGGCGTTCCACTAA PC*GDGSSINRYSAPA*AFH* -3.42 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21915 ATCEFWRECT 10 SLAY-screened peptide P265 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTACTTGTGAGTTCTGGAGGGAGTGCACCTAGCGGGCTTACGTTTACTCTGGTATTCTTTAA ATCEFWRECT*RAYVYSGIL* -3.416 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21916 MLCPHYSGHSRYTVRTFCKN 20 SLAY-screened peptide P266 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCTTTGTCCGCACTACTCTGGCCACAGTAGGTATACGGTTCGTACTTTTTGTAAGAACTAA MLCPHYSGHSRYTVRTFCKN* -3.415 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21917 LIILCYTTRSSIDTKYVPS 19 SLAY-screened peptide P267 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTATTATCCTTTGTTACACTACTCGTTCTAGTATCGACACGAAGTATGTTCCGTCCTAGTAA LIILCYTTRSSIDTKYVPS** -3.412 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21918 GVVCYLATDSPGTYPGSLSL 20 SLAY-screened peptide P268 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGTCGTGTGCTATCTTGCGACTGATTCGCCGGGCACTTATCCTGGGTCTCTCAGTTTGTAA GVVCYLATDSPGTYPGSLSL* -3.408 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21919 ELWPYFPSSYDLLCMPVDTY 20 SLAY-screened peptide P269 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCTCTGGCCCTACTTTCCGTCCTCCTACGATCTCCTCTGCATGCCTGTGGACACCTACTAA ELWPYFPSSYDLLCMPVDTY* -3.402 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21920 AGRNFPNCLCGLDAMTSSDI 20 SLAY-screened peptide P270 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGGGAGGAATTTCCCTAACTGCCTCTGTGGGCTGGATGCTATGACGAGTTCTGACATCTAA AGRNFPNCLCGLDAMTSSDI* -3.399 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21921 IPPQCPGILLPAYAFSVDSI 20 SLAY-screened peptide P271 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCCGCCTCAGTGCCCTGGTATCCTGCTTCCGGCGTATGCTTTTTCCGTTGACAGTATTTAA IPPQCPGILLPAYAFSVDSI* -3.391 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21922 RVHAVPPPGSHFPFLTRAGCN 21 SLAY-screened peptide P272 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGTTCATGCGGTTCCGCCGCCGGGCTCTCACTTTCCCTTCCTCACGCGGGCAGGATGTAAC RVHAVPPPGSHFPFLTRAGCN -3.387 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21923 HAGMDSADFIEYSASNKAHL 20 SLAY-screened peptide P273 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGCTGGCATGGATTCTGCGGATTTTATCGAGTATTCGGCGAGTAACAAGGCTCATCTTTAA HAGMDSADFIEYSASNKAHL* -3.375 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21924 SSFNWCPHRVFFCLSTKEVP 20 SLAY-screened peptide P274 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCTTTCAATTGGTGTCCGCATCGGGTTTTTTTCTGTCTTTCGACCAAGGAGGTCCCTTAA SSFNWCPHRVFFCLSTKEVP* -3.371 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21925 DICDNNIETNFQWTTDV 17 SLAY-screened peptide P275 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATATTTGTGATAATAATATTGAGACCAACTTTCAGTGGACCACTGACGTTTAGCTCCCTTAA DICDNNIETNFQWTTDV*LP* -3.368 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21926 QSTTLHTTCGYMSNENDEKG 20 SLAY-screened peptide P276 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGAGTACTACCTTGCATACTACTTGTGGTTATATGTCTAACGAGAATGACGAGAAGGGTTAA QSTTLHTTCGYMSNENDEKG* -3.363 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21927 FHILIARFRRARRFTIAVVIN 21 SLAY-screened peptide P277 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCACATCCTCATAGCCAGGTTCCGTCGAGCCCGTCGCTTCACGATTGCCGTCGTGATTAAC FHILIARFRRARRFTIAVVIN -3.355 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21928 SYYRIHQRIIVSSINAFTNY 20 SLAY-screened peptide P278 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTATTATCGTATCCACCAGCGGATCATTGTTTCGTCTATCAATGCTTTTACTAATTATTAA SYYRIHQRIIVSSINAFTNY* -3.342 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21929 PSPDLRAPSNHYNVYGTSH 19 SLAY-screened peptide P279 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTCGCCCGACCTCAGGGCTCCTTCTAATCATTATAATGTTTACGGTACCTCGCACTAGTAA PSPDLRAPSNHYNVYGTSH** -3.339 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21930 ANCTHAIYNNFCHHDHAYRT 20 SLAY-screened peptide P280 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAACTGTACCCATGCGATTTATAATAATTTCTGTCATCACGATCACGCCTACCGTACGTAA ANCTHAIYNNFCHHDHAYRT* -3.336 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21931 FLYHNFAMGWFIPGRPMYRA 20 SLAY-screened peptide P281 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCTCTACCACAACTTTGCTATGGGTTGGTTCATCCCCGGGCGCCCGATGTATAGGGCTTAA FLYHNFAMGWFIPGRPMYRA* -3.308 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21932 LMRTVGADSLSALFPDMGKP 20 SLAY-screened peptide P282 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCATGCGTACGGTCGGTGCTGATTCGCTGTCTGCTCTTTTTCCGGACATGGGCAAGCCCTAA LMRTVGADSLSALFPDMGKP* -3.299 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21933 EDPAPYTYIPHRYSSSISTH 20 SLAY-screened peptide P283 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGACCCCGCCCCTTACACTTATATTCCTCATAGGTATTCGAGTTCTATCTCGACCCATTAA EDPAPYTYIPHRYSSSISTH* -3.299 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21934 GI 2 SLAY-screened peptide P284 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTATTTAGAACCACGCTTTCTGGGTGCACAGTTATGTTCCGGGGAGGTAGACGGACACGTAA GI*NHAFWVHSYVPGR*TDT* -3.297 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21935 HSLSLSIRDSHINYECNNDS 20 SLAY-screened peptide P285 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAGTTTGTCGCTTTCCATTAGGGATTCTCATATCAATTATGAGTGCAATAACGATTCGTAA HSLSLSIRDSHINYECNNDS* -3.297 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21936 QNLITNFVGGNERHILPIAF 20 SLAY-screened peptide P286 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGAATCTCATTACTAATTTCGTTGGGGGTAACGAGCGGCATATCTTGCCCATTGCCTTTTAA QNLITNFVGGNERHILPIAF* -3.296 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21937 HANFSAPLTFLLTIRRRARG 20 SLAY-screened peptide P287 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGCCAACTTCAGTGCCCCTCTCACTTTTTTGCTCACGATTCGCCGGCGGGCTAGGGGCTAA HANFSAPLTFLLTIRRRARG* -3.294 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21938 TITPAIPLPRIRSPPSCTFVT 21 SLAY-screened peptide P288 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCATTACTCCAGCGATCCCCTTGCCTCGAATTAGATCGCCACCAAGCTGTACATTCGTAACT TITPAIPLPRIRSPPSCTFVT -3.293 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21939 PNRQHLNFHYFCLMLHPPMP 20 SLAY-screened peptide P289 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAACCGTCAGCATCTTAATTTTCATTATTTCTGCCTTATGCTCCATCCGCCCATGCCGTAA PNRQHLNFHYFCLMLHPPMP* -3.293 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21940 QGNHTLNPSFSANNSFCAIT 20 SLAY-screened peptide P290 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGGCAATCATACTCTTAATCCTAGCTTTTCCGCCAATAATAGCTTTTGCGCTATTACCTAA QGNHTLNPSFSANNSFCAIT* -3.29 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21941 LHSTSIHAMSSHSRTINGKH 20 SLAY-screened peptide P291 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCATAGCACTTCTATCCATGCTATGTCTAGCCATAGTCGTACTATCAACGGCAAGCACTAA LHSTSIHAMSSHSRTINGKH* -3.282 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21942 IFDHDTHCSCNFHFIANGSW 20 SLAY-screened peptide P292 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTTTCGATCATGATACTCACTGTAGTTGCAATTTTCACTTTATCGCGAACGGCTCTTGGTAA IFDHDTHCSCNFHFIANGSW* -3.281 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21943 RKCVISVARRNRRANIKILCN 21 SLAY-screened peptide P293 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAAGTGCGTTATCTCCGTGGCGCGCCGTAATCGTAGGGCCAACATCAAGATCTTATGTAAC RKCVISVARRNRRANIKILCN -3.271 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21944 EPTILPDSDNSWIYTLDFTK 20 SLAY-screened peptide P294 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCCCACTATTCTCCCCGACTCCGATAATTCTTGGATTTATACTCTTGATTTTACTAAGTAA EPTILPDSDNSWIYTLDFTK* -3.266 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21945 HNSFDSLLFYRKMDECVVGA 20 SLAY-screened peptide P295 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAACTCTTTTGATTCGCTGCTCTTTTACCGTAAGATGGACGAGTGTGTTGTTGGGGCCTAA HNSFDSLLFYRKMDECVVGA* -3.262 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21946 LSMQ 4 SLAY-screened peptide P296 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCGATGCAGTAGTTCCCGGATCTTAGTCCGCGGTTGCGTTCGCATAGTGATACGTAACTG LSMQ*FPDLSPRLRSHSDT*L -3.254 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21947 IEAPSTVPPYPFTQSCYESW 20 SLAY-screened peptide P297 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGAGGCGCCCTCTACGGTTCCTCCTTATCCGTTTACTCAGAGCTGTTACGAGAGCTGGTAA IEAPSTVPPYPFTQSCYESW* -3.25 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21948 ISANYRSVFDSQHRVNDLLA 20 SLAY-screened peptide P298 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAGCGCGAACTATCGCAGTGTCTTTGATAGCCAGCATCGTGTCAATGATCTTCTCGCCTAA ISANYRSVFDSQHRVNDLLA* -3.246 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21949 LAMPFIKYPLNSRDGVCTHP 20 SLAY-screened peptide P299 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGCTATGCCGTTTATTAAGTACCCGTTGAACAGCCGTGACGGCGTTTGCACGCACCCCTAA LAMPFIKYPLNSRDGVCTHP* -3.246 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21950 ILLRNTGFITRVFQTCVEPV 20 SLAY-screened peptide P300 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCTCCTTCGCAATACCGGTTTTATTACTCGGGTGTTCCAGACTTGCGTTGAGCCCGTTTAA ILLRNTGFITRVFQTCVEPV* -3.246 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21951 FTMYVVLLHIRQNL 14 SLAY-screened peptide P301 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTACCATGTACGTGGTCCTTCTCCATATTCGCCAGAATCTCTAGGCCCCGGACGCCTGTTAA FTMYVVLLHIRQNL*APDAC* -3.244 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21952 SLDYNHRIDLSVLPYCLGPT 20 SLAY-screened peptide P302 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCTCGATTATAATCACCGTATCGACCTTTCTGTTCTTCCGTACTGCCTCGGTCCGACCTAA SLDYNHRIDLSVLPYCLGPT* -3.243 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21953 PRPRWPPPTTHTIVTPQDTL 20 SLAY-screened peptide P303 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGGCCGAGGTGGCCCCCTCCCACTACTCACACTATTGTTACTCCCCAGGACACGTTGTAA PRPRWPPPTTHTIVTPQDTL* -3.24 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21954 RLHATTYMHMHRDLMNFAFL 20 SLAY-screened peptide P304 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCTGCATGCTACCACGTATATGCATATGCATCGTGACTTGATGAACTTCGCGTTCCTGTAA RLHATTYMHMHRDLMNFAFL* -3.239 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21955 APYRRCSKNRLVLAS 15 SLAY-screened peptide P305 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCCCTATCGGCGCTGCTCTAAGAACCGCTTGGTTCTGGCTTCCTAGCAGACGACGTAGTAA APYRRCSKNRLVLAS*QTT** -3.23 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21956 PRRAYFNFNGGSYDTVTISF 20 SLAY-screened peptide P306 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGGAGGGCGTACTTCAACTTCAACGGCGGTAGTTACGATACGGTCACTATTAGTTTCTAA PRRAYFNFNGGSYDTVTISF* -3.218 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21957 LGEAYECSTFNFGST 15 SLAY-screened peptide P307 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGGCGAGGCTTATGAGTGCAGCACTTTCAATTTTGGCTCGACTTAGCACACTGTCGCTAAC LGEAYECSTFNFGST*HTVAN -3.209 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21958 VITPDRSGHFTFDHYYYWAS 20 SLAY-screened peptide P308 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCATCACGCCGGACCGTTCCGGCCACTTTACGTTCGATCACTATTATTACTGGGCCAGTTAA VITPDRSGHFTFDHYYYWAS* -3.206 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21959 IGHLYHSYVSSCSRSGVGMS 20 SLAY-screened peptide P309 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGGTCATCTTTACCATAGCTACGTGTCTAGCTGCTCTAGGTCCGGTGTGGGTATGTCTTAA IGHLYHSYVSSCSRSGVGMS* -3.204 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21960 NAWYTVHYTHNFVIS 15 SLAY-screened peptide P310 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGCGTGGTATACTGTTCACTACACTCATAATTTTGTCATCAGCTAGGACCATACGCAGTAA NAWYTVHYTHNFVIS*DHTQ* -3.202 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21961 DFLALSHYTCCSSNHIPPCH 20 SLAY-screened peptide P311 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTTTTTGGCGCTCAGTCACTATACGTGTTGCTCTTCTAATCATATCCCTCCTTGTCACTAA DFLALSHYTCCSSNHIPPCH* -3.202 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21962 YSTMFHDHPGMGGFDRPPQL 20 SLAY-screened peptide P312 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTCTACCATGTTCCATGATCACCCCGGTATGGGTGGTTTTGATCGTCCGCCCCAGCTGTAA YSTMFHDHPGMGGFDRPPQL* -3.201 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21963 MQPHRRNYNTYSLFTDPSDT 20 SLAY-screened peptide P313 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCAGCCTCACCGCCGCAATTATAATACGTATAGTCTTTTTACTGACCCTAGCGATACCTAA MQPHRRNYNTYSLFTDPSDT* -3.198 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21964 HTVLPLYRTVTSKCSHTMGV 20 SLAY-screened peptide P314 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACTGTCCTTCCTCTGTATCGGACCGTCACTTCTAAGTGCTCTCACACTATGGGTGTCTAA HTVLPLYRTVTSKCSHTMGV* -3.194 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21965 PYNVYHSFKHYHIYDDNWVP 20 SLAY-screened peptide P315 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTACAACGTCTATCACAGTTTTAAGCATTACCATATCTATGACGACAATTGGGTGCCTTAA PYNVYHSFKHYHIYDDNWVP* -3.194 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21966 CLHCLCYSGSDCDNIYSFIS 20 SLAY-screened peptide P316 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTTGCATTGTTTGTGCTATTCTGGGAGTGACTGCGACAATATTTACTCTTTCATTTCCTAA CLHCLCYSGSDCDNIYSFIS* -3.192 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21967 LHAIFLHCCKIHAQCVTLYT 20 SLAY-screened peptide P317 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACGCCATTTTCCTGCATTGTTGCAAGATCCACGCTCAGTGCGTCACTTTGTATACTTAA LHAIFLHCCKIHAQCVTLYT* -3.19 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21968 PALHYVNFERYMPSDNRRL 19 SLAY-screened peptide P318 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGCTTTGCATTACGTTAACTTCGAGCGGTATATGCCTTCGGACAATCGCCGGCTGTAGTAA PALHYVNFERYMPSDNRRL** -3.183 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21969 NLSLPDYNICMHREHPTILL 20 SLAY-screened peptide P319 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCTGTCGCTTCCCGATTATAACATCTGCATGCACCGCGAGCACCCTACCATTCTCCTGTAA NLSLPDYNICMHREHPTILL* -3.181 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21970 MRFNPTHIYSVPLMTLAPLIN 21 SLAY-screened peptide P320 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCGCTTTAACCCTACTCATATTTATAGCGTACCGTTGATGACACTGGCACCTCTAATTAAC MRFNPTHIYSVPLMTLAPLIN -3.179 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21971 DISHRVRSSDLFLHRPCISY 20 SLAY-screened peptide P321 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACATCAGTCACCGTGTGCGTTCTTCCGATTTGTTCCTTCATCGTCCTTGCATTTCTTATTAA DISHRVRSSDLFLHRPCISY* -3.172 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21972 SLHYGPWHDIFNTPMSHYLW 20 SLAY-screened peptide P322 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCTGCACTACGGTCCCTGGCATGATATTTTCAATACGCCCATGTCTCACTATCTTTGGTAA SLHYGPWHDIFNTPMSHYLW* -3.169 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21973 PCLYDSNCYCFNYCHRPNGE 20 SLAY-screened peptide P323 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGTCTCTATGATTCTAATTGCTATTGTTTCAATTACTGTCACAGGCCTAATGGCGAGTAA PCLYDSNCYCFNYCHRPNGE* -3.166 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21974 IATPCNLLDDVFDYTLATDS 20 SLAY-screened peptide P324 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGCGACGCCCTGTAATTTGTTGGATGATGTTTTTGATTATACGCTGGCGACTGACTCTTAA IATPCNLLDDVFDYTLATDS* -3.165 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21975 TSYLRYTPHTTLTIFIFVCPN 21 SLAY-screened peptide P325 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCAGCTACCTGCGTTATACTCCGCACACCACTCTCACTATTTTCATTTTTGTGTGTCCTAAC TSYLRYTPHTTLTIFIFVCPN -3.155 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21976 CAASYIQDPASYACFNLKSA 20 SLAY-screened peptide P326 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGCTGCTAGTTACATTCAGGATCCGGCTTCGTACGCCTGCTTTAACCTTAAGAGCGCTTAA CAASYIQDPASYACFNLKSA* -3.154 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21977 NSLA 4 SLAY-screened peptide P327 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAGCTTGGCTTAGGAGCCTACGTTCTACGATGGTATTTATTATATTCCTAAAACTAGTAAC NSLA*EPTFYDGIYYIPKTSN -3.152 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21978 NYTRTHIQILAVPVITF 17 SLAY-screened peptide P328 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTATACGCGTACTCACATTCAGATCCTGGCTGTTCCGGTGATTACTTTTTAGTATTATTAA NYTRTHIQILAVPVITF*YY* -3.152 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21979 LPETEALPYRCNIWITLNKE 20 SLAY-screened peptide P329 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCTGAGACCGAGGCTTTGCCTTATCGCTGTAATATCTGGATTACTCTTAATAAGGAGTAA LPETEALPYRCNIWITLNKE* -3.148 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21980 LNTSSRINCFYIDPPDHLFS 20 SLAY-screened peptide P330 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAATACTAGTAGCCGCATTAATTGTTTTTACATTGACCCCCCTGACCATCTGTTTTCTTAA LNTSSRINCFYIDPPDHLFS* -3.147 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21981 RTNLLVMFSFLACMSIPMRI 20 SLAY-screened peptide P331 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACTAATTTGCTGGTTATGTTCTCCTTTTTGGCCTGTATGTCTATCCCCATGCGCATTTAA RTNLLVMFSFLACMSIPMRI* -3.144 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21982 EHAS 4 SLAY-screened peptide P332 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCACGCGAGCTAGACGTTCGATAATTCTCTTATCTATCCTCATCGTTGCATTTTTGATTAA EHAS*TFDNSLIYPHRCIFD* -3.144 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21983 YFPWKGIDY 9 SLAY-screened peptide P333 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTTCCCTTGGAAGGGTATTGATTATTAGACTGACGTCGACATGGCGTATTAGATTGTTTAA YFPWKGIDY*TDVDMAY*IV* -3.135 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21984 CNQSPFIYIACWGNGVIVHL 20 SLAY-screened peptide P334 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAACCAGTCTCCGTTCATTTACATCGCTTGTTGGGGTAATGGTGTTATTGTTCATCTTTAA CNQSPFIYIACWGNGVIVHL* -3.134 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21985 WIPPPQASDTTDGVASSKYD 20 SLAY-screened peptide P335 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGATTCCGCCTCCGCAGGCTAGTGATACTACTGATGGCGTTGCTAGTTCTAAGTACGATTAA WIPPPQASDTTDGVASSKYD* -3.133 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21986 HLDLHLNKSLHITLWYV 17 SLAY-screened peptide P336 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTTGGATTTGCACCTCAATAAGAGTCTCCATATTACTCTCTGGTACGTCTAGGGCTTAACT HLDLHLNKSLHITLWYV*GLT -3.133 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21987 VHTLQTYKDAALDTLYRVLFN 21 SLAY-screened peptide P337 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCATACGCTGCAGACGTACAAGGACGCCGCTCTTGATACTCTTTACAGGGTCCTGTTTAAC VHTLQTYKDAALDTLYRVLFN -3.132 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21988 AQAPDSRYDNTFIGHIDVMK 20 SLAY-screened peptide P338 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCAGGCGCCTGATAGTCGCTATGATAATACTTTTATCGGTCACATTGACGTTATGAAGTAA AQAPDSRYDNTFIGHIDVMK* -3.127 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21989 RKSYSLHICANDYNDKNLGPN 21 SLAY-screened peptide P339 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGAAGAGCTACTCTCTTCACATTTGCGCGAATGATTATAACGATAAGAATCTTGGACCTAAC RKSYSLHICANDYNDKNLGPN -3.124 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21990 NKCRPISKADVL 12 SLAY-screened peptide P340 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAAGTGCCGCCCCATCTCGAAGGCTGACGTGTTGTAGATGGTTGACTCTAACAGCACTTAA NKCRPISKADVL*MVDSNST* -3.124 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21991 AHPRPVSAP 9 SLAY-screened peptide P341 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCATCCCCGTCCGGTGTCCGCTCCTTAGCACCATCACCCTTAGAACACGACCGTGCACTAA AHPRPVSAP*HHHP*NTTVH* -3.12 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21992 SSDYSDPLSWARSTCDNRNP 20 SLAY-screened peptide P342 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTCGGATTACAGTGATCCTCTTTCTTGGGCGCGGTCTACTTGCGACAATAGGAACCCTTAA SSDYSDPLSWARSTCDNRNP* -3.117 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21993 AFTNMLITAFCNPIYAMTVDL 21 SLAY-screened peptide P343 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGTTCACGAACATGCTTATTACGGCTTTTTGCAACCCTATCTATGCTATGACCGTCGACCTG AFTNMLITAFCNPIYAMTVDL -3.114 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21994 VCDYHYNIHCLRRR 14 SLAY-screened peptide P344 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTGTGATTACCATTATAACATCCATTGTCTTCGCCGTCGTTAGTAGCCTCATAATAATTAA VCDYHYNIHCLRRR**PHNN* -3.106 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21995 IIDSGTQPGAFYLVMFRIVQ 20 SLAY-screened peptide P345 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTATTGATAGTGGTACTCAGCCTGGTGCTTTTTACCTGGTTATGTTCCGTATTGTTCAGTAA IIDSGTQPGAFYLVMFRIVQ* -3.102 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21996 GTSSPRKPIHNYRKENITND 20 SLAY-screened peptide P346 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTACTAGCTCTCCTCGTAAGCCCATTCATAACTACAGGAAGGAGAATATCACGAACGACTAA GTSSPRKPIHNYRKENITND* -3.101 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21997 VPFLPGIWVLPRPVRIASFAN 21 SLAY-screened peptide P347 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCCATTTTTGCCCGGTATATGGGTACTACCAAGACCTGTGCGCATCGCGTCGTTTGCTAAC VPFLPGIWVLPRPVRIASFAN -3.101 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21998 SGEDR 5 SLAY-screened peptide P348 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGGGGAGGACCGGTAGCCTCTCTCTGTCCCTATTGTTCTTGACCCTGATCAGGCTTCGTAA SGEDR*PLSVPIVLDPDQAS* -3.1 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP21999 MPRVHPTVDRNALYLIPVIN 20 SLAY-screened peptide P349 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCCTCGTGTCCACCCTACGGTGGATCGTAACGCTCTTTATCTCATTCCCGTTATTAACTAA MPRVHPTVDRNALYLIPVIN* -3.091 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22000 APRIDDIR 8 SLAY-screened peptide P350 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCCGCGCATTGATGACATTAGGTAGGGTCGCTCTAACAATATTGGGGCGCTGTTTTCGTAA APRIDDIR*GRSNNIGALFS* -3.09 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22001 AQSDWNTSVGSFHYSCAILY 20 SLAY-screened peptide P351 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCAGAGTGATTGGAACACTTCGGTCGGTAGTTTCCATTATAGTTGTGCTATCTTGTACTAA AQSDWNTSVGSFHYSCAILY* -3.086 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22002 LPFRWGGSVRYPMRRCTTLV 20 SLAY-screened peptide P352 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCTTTTCGTTGGGGTGGGTCTGTGCGTTACCCTATGCGCCGTTGTACTACTCTCGTCTAA LPFRWGGSVRYPMRRCTTLV* -3.08 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22003 RAHSSHRYNHVVYSISSYIY 20 SLAY-screened peptide P353 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCTCATAGCTCGCATCGTTATAACCATGTTGTCTACTCTATTTCTAGTTATATTTATTAA RAHSSHRYNHVVYSISSYIY* -3.072 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22004 AHRSGNFFPIYPSSLPMAFY 20 SLAY-screened peptide P354 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCATCGTTCGGGGAATTTTTTTCCCATTTACCCTAGTTCTCTTCCTATGGCTTTTTACTAA AHRSGNFFPIYPSSLPMAFY* -3.071 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22005 IVGDLLNKGFNSGDSFT 17 SLAY-screened peptide P355 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGTTGGCGACCTTCTTAATAAGGGGTTTAATTCGGGCGATAGCTTCACTTAGGCTAGTTAC IVGDLLNKGFNSGDSFT*ASY -3.066 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22006 PVNEDIQ 7 SLAY-screened peptide P356 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGTCAACGAGGACATCCAGTAGACCCTTACGACAACCATTACCACATGAGTCTTAGCTAAC PVNEDIQ*TLTTTITT*VLAN -3.065 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22007 RENRPSHWFVTQLCYLLCRH 20 SLAY-screened peptide P357 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGAGAATAGGCCCAGCCATTGGTTTGTCACTCAGCTTTGCTATTTGCTTTGCCGCCACTAA RENRPSHWFVTQLCYLLCRH* -3.065 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22008 PATTNGSR 8 SLAY-screened peptide P358 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGCGACTACTAATGGCTCTCGGTAGACGTGCGCTGACATTTGCAATTCCCTGGATTCGTAA PATTNGSR*TCADICNSLDS* -3.065 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22009 STLLKSYHIFAYSMLPFWYH 20 SLAY-screened peptide P359 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTACTTTGCTTAAGTCCTATCATATCTTTGCGTACAGTATGTTGCCTTTTTGGTACCATTAA STLLKSYHIFAYSMLPFWYH* -3.064 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22010 GSKGSCTLYFNLIGFWTPTD 20 SLAY-screened peptide P360 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTCGAAGGGCTCTTGCACGCTGTACTTTAATCTTATCGGCTTTTGGACTCCGACGGACTAA GSKGSCTLYFNLIGFWTPTD* -3.063 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22011 RCHLHCYTTLNDPPHHRVS 19 SLAY-screened peptide P361 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTGTCACCTTCACTGTTACACTACCTTGAACGACCCTCCCCACCATCGTGTCAGCTAGTAA RCHLHCYTTLNDPPHHRVS** -3.061 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22012 LPEISHTRR 9 SLAY-screened peptide P362 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCCGAGATCAGCCACACTCGTCGTTAGATCCCGAAGGAGCGCTGTCACCGTTACAATTAA LPEISHTRR*IPKERCHRYN* -3.059 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22013 HTIVLLTLVTLRRRLYSFLK 20 SLAY-screened peptide P363 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACCATCGTCTTGCTCACCCTCGTCACGCTCCGGCGCCGGCTCTATAGCTTCCTCAAGTAA HTIVLLTLVTLRRRLYSFLK* -3.058 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22014 AHLSNSIDPFHAGSVHFTPD 20 SLAY-screened peptide P364 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCATCTTAGCAACAGTATTGATCCTTTCCATGCTGGCAGCGTCCACTTCACCCCTGACTAA AHLSNSIDPFHAGSVHFTPD* -3.056 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22015 LKWYCHFNSTQNLRAQTNIG 20 SLAY-screened peptide P365 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAAGTGGTACTGTCATTTTAATAGTACCCAGAATCTGCGCGCCCAGACGAACATCGGCTAA LKWYCHFNSTQNLRAQTNIG* -3.055 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22016 CGGLLAWTGPLSECIQFWLL 20 SLAY-screened peptide P366 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGGTGGCTTGCTGGCGTGGACCGGCCCGCTGAGCGAGTGCATTCAGTTCTGGCTTCTTTAA CGGLLAWTGPLSECIQFWLL* -3.052 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22017 PP 2 SLAY-screened peptide P367 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCGTAGCCGATCACGACGCATACGTAGTCGCCGAGTAATCTGCTGGCTGTGATGATGTAA PP*PITTHT*SPSNLLAVMM* -3.052 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22018 LRGLLSFSSYQMVMDGDTITN 21 SLAY-screened peptide P368 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCGCGGCCTTCTTAGCTTCTCTTCCTATCAGATGGTTATGGACGGTGATACTATTACTAAC LRGLLSFSSYQMVMDGDTITN -3.052 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22019 TTLFNVSLHMVNTSGSTGTN 20 SLAY-screened peptide P369 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCACCCTGTTTAACGTTTCCTTGCATATGGTTAATACTAGCGGTTCTACTGGTACTAACTAA TTLFNVSLHMVNTSGSTGTN* -3.05 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22020 AGNWLMAGLSLAPARPSPNG 20 SLAY-screened peptide P370 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGGCAACTGGCTTATGGCCGGTCTCAGCCTTGCTCCTGCGCGTCCTAGCCCTAATGGCTAA AGNWLMAGLSLAPARPSPNG* -3.048 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22021 LSLHCDIGFNANNTLSTDYI 20 SLAY-screened peptide P371 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCCTTGCATTGTGATATTGGTTTTAACGCCAATAATACGCTGTCCACCGATTACATTTAA LSLHCDIGFNANNTLSTDYI* -3.046 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22022 FLVAVRINFNLNIRFYIDLS 20 SLAY-screened peptide P372 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCTTGTCGCCGTGCGCATTAATTTTAACCTCAATATCCGCTTTTATATCGACCTCTCTTAA FLVAVRINFNLNIRFYIDLS* -3.042 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22023 FIPHHNHSLAYETIVSGRDP 20 SLAY-screened peptide P373 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCATTCCTCATCATAATCATAGCCTCGCTTACGAGACGATTGTTAGTGGGCGTGACCCGTAA FIPHHNHSLAYETIVSGRDP* -3.038 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22024 HHRSRKMYNWNHNEANRHYQ 20 SLAY-screened peptide P374 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCATAGGAGTCGCAAGATGTATAACTGGAACCATAACGAGGCTAACCGTCACTATCAGTAA HHRSRKMYNWNHNEANRHYQ* -3.036 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22025 IICLELANDDLCCKCHSSDD 20 SLAY-screened peptide P375 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTATTTGCCTGGAGCTGGCTAATGATGATCTCTGTTGCAAGTGTCATTCTTCCGACGACTAA IICLELANDDLCCKCHSSDD* -3.035 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22026 LAESALLRGNNSCNLTFIRN 20 SLAY-screened peptide P376 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGCCGAGAGCGCTCTGTTGCGTGGCAATAATAGTTGTAATTTGACTTTTATCAGGAACTAA LAESALLRGNNSCNLTFIRN* -3.035 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22027 YSHVCKTNTSHCYTFHYNGF 20 SLAY-screened peptide P377 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTCTCACGTTTGTAAGACTAATACTTCCCATTGTTATACTTTTCATTACAATGGCTTCTAA YSHVCKTNTSHCYTFHYNGF* -3.033 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22028 SQP 3 SLAY-screened peptide P378 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCAGCCTTAGCTGATCCCCATTACTAACTAGCATTCTAGTTCGTTCATGCCCGCCGACTAA SQP*LIPITN*HSSSFMPAD* -3.022 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22029 KHRF 4 SLAY-screened peptide P379 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCATCGCTTTTAGCATGCTTATGCGCCGTGGTACGCTTTTAACTTTGTCTGTCGCTATTAA KHRF*HAYAPWYAFNFVCRY* -3.018 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22030 LCALTQASTLSNNHTTHLAT 20 SLAY-screened peptide P380 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTGCGCCCTCACTCAGGCCTCTACTCTTAGTAACAACCATACTACGCATTTGGCTACGTAA LCALTQASTLSNNHTTHLAT* -3.012 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22031 WFAPCKSAAIHAF 13 SLAY-screened peptide P381 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGTTTGCCCCCTGCAAGAGTGCCGCCATCCATGCTTTTTAGCTTCTCAACCATGAGGCTTAA WFAPCKSAAIHAF*LLNHEA* -3.01 0.000101 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22032 PRTLLTTALRILYTKGLLGD 20 SLAY-screened peptide P382 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCGCACTTTGCTTACTACTGCGCTTCGCATTCTTTATACTAAGGGGCTTCTTGGCGACTAA PRTLLTTALRILYTKGLLGD* -3.008 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22033 VFSSAYRADAKGTSSFNSTQ 20 SLAY-screened peptide P383 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTTCTCGTCGGCGTATCGTGCTGACGCTAAGGGCACGTCGAGTTTCAACTCGACGCAGTAA VFSSAYRADAKGTSSFNSTQ* -3.008 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22034 PNISTGPSFILPLLLGCIAFN 21 SLAY-screened peptide P384 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAACATTTCTACCGGTCCAAGTTTTATACTACCCCTTCTCCTGGGTTGCATCGCATTTAAC PNISTGPSFILPLLLGCIAFN -3.008 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22035 YSTSSCSCDYQSSSYR 16 SLAY-screened peptide P385 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAGTACTTCGAGTTGTTCTTGTGATTACCAATCGTCATCTTATCGGTAACTGAGTAAGTCG YSTSSCSCDYQSSSYR*LSKS -3.006 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22036 HAALGCQHYPNMRTELDHTK 20 SLAY-screened peptide P386 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGCGGCCCTCGGTTGTCAGCATTATCCGAACATGCGGACTGAGCTCGATCACACTAAGTAA HAALGCQHYPNMRTELDHTK* -3.005 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22037 ASSVSSFVLYSARSFNNSSH 20 SLAY-screened peptide P387 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTAGTTCTGTGTCCAGTTTTGTGCTCTATTCTGCTCGTTCTTTCAATAATAGTTCTCATTAA ASSVSSFVLYSARSFNNSSH* -3.004 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22038 VALDTTFSHRSPP 13 SLAY-screened peptide P388 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGCGCTTGACACTACTTTTAGTCATCGGAGTCCCCCTTAGACCTTTTATATTAAGAATTAA VALDTTFSHRSPP*TFYIKN* -3.002 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22039 LVYTDLYGFFSDLRPRNQDD 20 SLAY-screened peptide P389 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGGTGTACACTGACCTTTACGGTTTTTTTAGCGACCTTCGCCCTAGGAACCAGGACGATTAA LVYTDLYGFFSDLRPRNQDD* -3.001 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22040 KNVNHSVIVNPNFDPNTVTR 20 SLAY-screened peptide P390 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGAATGTTAATCATAGTGTCATCGTCAACCCGAATTTCGATCCGAATACTGTTACGAGGTAA KNVNHSVIVNPNFDPNTVTR* -2.997 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22041 STTLYLPGLNRIRTNDFMIT 20 SLAY-screened peptide P391 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGACCACTCTGTATCTGCCTGGTCTTAACCGGATCAGGACGAACGATTTTATGATCACCTAA STTLYLPGLNRIRTNDFMIT* -2.995 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22042 STLINVFDR 9 SLAY-screened peptide P392 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTACCCTCATTAACGTTTTCGACAGGTAGGTGTATAAGTCGTGGCCGATTTACTGGTCCTAA STLINVFDR*VYKSWPIYWS* -2.993 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22043 LVTDAMWHGLHVSRCHSHYY 20 SLAY-screened peptide P393 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGTTACTGATGCCATGTGGCATGGGCTTCATGTTTCGCGTTGTCATAGTCACTACTACTAA LVTDAMWHGLHVSRCHSHYY* -2.988 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22044 GPIHDVLRMIRSSGTTHFYS 20 SLAY-screened peptide P394 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCCTATCCATGATGTGCTTAGGATGATTCGTAGCTCCGGGACGACCCACTTTTATAGCTAA GPIHDVLRMIRSSGTTHFYS* -2.986 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22045 LPTNSIRRDGLSADHHRYIRN 21 SLAY-screened peptide P395 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCCACTAATAGTATTCGTCGTGACGGCCTCAGCGCTGATCATCATCGCTACATCCGTAAC LPTNSIRRDGLSADHHRYIRN -2.985 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22046 LMRSVFLNMCIPSDYMDPSVP 21 SLAY-screened peptide P396 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCATGCGTAGTGTCTTTCTTAATATGTGCATCCCTTCTGACTATATGGACCCCAGTGTGCCC LMRSVFLNMCIPSDYMDPSVP -2.98 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22047 NKNPIRLSFYPHNYYLYSSV 20 SLAY-screened peptide P397 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAAGAATCCCATCAGGCTTAGTTTCTATCCTCATAATTATTATTTGTATTCCAGTGTTTAA NKNPIRLSFYPHNYYLYSSV* -2.977 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22048 TSLCFVIILNLKSDMAG 17 SLAY-screened peptide P398 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTCTCTGTGCTTCGTCATTATTCTTAATCTCAAGAGTGATATGGCGGGCTAGTGTTCGTAA TSLCFVIILNLKSDMAG*CS* -2.975 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22049 HNNPPAPNGPHSTFIADNAS 20 SLAY-screened peptide P399 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAACAATCCGCCGGCCCCTAACGGGCCTCATAGTACGTTTATCGCTGATAATGCTAGTTAA HNNPPAPNGPHSTFIADNAS* -2.97 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22050 WTYFHSNPHEYQLNLLIANM 20 SLAY-screened peptide P400 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGACCTACTTTCATAGTAACCCGCATGAGTATCAGCTTAACCTTCTTATTGCCAATATGTAA WTYFHSNPHEYQLNLLIANM* -2.966 6.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22051 LIFTLQNRLQPVAMHKPCYS 20 SLAY-screened peptide P401 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTATCTTTACCTTGCAGAACCGCCTCCAGCCGGTGGCCATGCATAAGCCCTGTTACTCCTAA LIFTLQNRLQPVAMHKPCYS* -2.962 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22052 PDHNNHT 7 SLAY-screened peptide P402 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGATCACAATAATCATACGTAGTTCATGTTTAGTACCACTACGTTGTACCATGAGCCGTAA PDHNNHT*FMFSTTTLYHEP* -2.96 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22053 YHNHSHD 7 SLAY-screened peptide P403 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCATAATCACTCTCATGATTAGGATGCCGCCCTGTACCTTGGTTCCATGTAGATGATCTAA YHNHSHD*DAALYLGSM*MI* -2.958 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22054 ALTQHRLGLRNIPQNLYIMV 20 SLAY-screened peptide P404 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCTCACTCAGCATCGTCTCGGGCTTAGGAATATCCCTCAGAATCTCTATATTATGGTCTAA ALTQHRLGLRNIPQNLYIMV* -2.955 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22055 ASFTHPPIMAPPIYASSEVE 20 SLAY-screened peptide P405 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGTCTTTTACTCATCCTCCTATCATGGCCCCGCCTATCTACGCTTCTAGTGAGGTTGAGTAA ASFTHPPIMAPPIYASSEVE* -2.955 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22056 HTLQQLRCPHCSLSNNSMVY 20 SLAY-screened peptide P406 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACACTCTTCAGCAGCTTCGTTGTCCGCATTGCAGCCTTAGCAATAATTCTATGGTGTACTAA HTLQQLRCPHCSLSNNSMVY* -2.951 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22057 TISRGNSPPSANTALLMNYI 20 SLAY-screened peptide P407 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGATTTCTCGCGGTAATTCGCCTCCTTCTGCTAATACTGCGCTTCTCATGAACTACATTTAA TISRGNSPPSANTALLMNYI* -2.948 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22058 TSPMQSLRLLTSISLKNRVM 20 SLAY-screened peptide P408 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTCTCCTATGCAGTCCCTGCGTCTCCTCACGAGCATCTCGTTGAAGAACAGGGTTATGTAA TSPMQSLRLLTSISLKNRVM* -2.945 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22059 IAVGPVGRIRFPRLTFRFTL 20 SLAY-screened peptide P409 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGCCGTGGGCCCTGTGGGCCGCATCCGTTTTCCTAGGCTCACTTTTCGCTTTACCCTGTAA IAVGPVGRIRFPRLTFRFTL* -2.944 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22060 SGRQTGNHNCYMSLQLLTIC 20 SLAY-screened peptide P410 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGGCCGTCAGACGGGGAACCATAATTGTTATATGTCTCTTCAGCTCCTGACCATCTGCTAA SGRQTGNHNCYMSLQLLTIC* -2.939 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22061 QSAAGMPSVD 10 SLAY-screened peptide P411 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTCCGCCGCTGGTATGCCTAGCGTTGATTAGGTGTTGTATCCTCATGTGCGTTCCGTTTAA QSAAGMPSVD*VLYPHVRSV* -2.937 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22062 PRIFCILLPRPCSGHVFYAS 20 SLAY-screened peptide P412 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGGATTTTCTGTATTTTGCTTCCTCGGCCCTGTTCTGGCCACGTGTTCTATGCTAGTTAA PRIFCILLPRPCSGHVFYAS* -2.927 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22063 VSFFTVPLWHCLPSDLLALN 20 SLAY-screened peptide P413 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTCTTTTTTTACCGTGCCGCTGTGGCATTGTCTGCCGAGTGATCTTTTGGCGCTGAATTAA VSFFTVPLWHCLPSDLLALN* -2.919 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22064 ALRTMY 6 SLAY-screened peptide P414 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCTTCGCACTATGTATTAGTTTTACAATTATATCTTACCTGTAAGAACAATAGGAATTAAC ALRTMY*FYNYILPVRTIGIN -2.911 4.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22065 RTLPFCAPGIVLTLI 15 SLAY-screened peptide P415 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGAACGTTACCATTTTGCGCACCGGGAATAGTTCTTACACTTATATGACCATCTTGTTCTAAC RTLPFCAPGIVLTLI*PSCSN -2.911 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22066 CVVDPLY 7 SLAY-screened peptide P416 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGTCGTGGACCCTCTCTATTAGTATTGGGCCATCCTCTCCTTTTGCCGTAGGTACCCTTAA CVVDPLY*YWAILSFCRRYP* -2.903 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22067 RPIIPYSAHSYLCVTTYNPT 20 SLAY-screened peptide P417 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCCCATTATTCCTTATAGCGCTCACTCTTATCTGTGTGTCACCACCTACAATCCTACGTAA RPIIPYSAHSYLCVTTYNPT* -2.902 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22068 LRVPIVPISS 10 SLAY-screened peptide P418 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCGTGTGCCCATTGTTCCTATTTCGAGTTAGTGTCAGAACGTCTTCAATGGCGACTCTTAA LRVPIVPISS*CQNVFNGDS* -2.901 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22069 YRHTAN 6 SLAY-screened peptide P419 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAGGCACACTGCTAACTAGCTCCGTGAGTATCTTGGGTAGGCGACGCTGGAGAGTGCTTAA YRHTAN*LREYLG*ATLESA* -2.899 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22070 RAN 3 SLAY-screened peptide P420 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGCGAATTAGCACCTCCCTCATGAGACTCATGATACGGTTGAGTCGTCCATGAATAGCTAA RAN*HLPHETHDTVESSMNS* -2.892 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22071 MLDYFLGHSYLSLVDEDPNR 20 SLAY-screened peptide P421 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTTGGACTACTTTCTCGGTCACAGTTATCTCAGCCTCGTTGATGAGGATCCTAACAGGTAA MLDYFLGHSYLSLVDEDPNR* -2.888 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22072 APLFFGLCIVCTTDGRRKSF 20 SLAY-screened peptide P422 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCCTTGTTTTTTGGCCTTTGTATCGTCTGTACTACGGATGGTCGCCGGAAGAGCTTTTAA APLFFGLCIVCTTDGRRKSF* -2.885 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22073 TCVDITATICAVTWIVIDFA 20 SLAY-screened peptide P423 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGTGTTGATATTACTGCGACTATTTGTGCTGTTACGTGGATTGTGATTGATTTTGCCTAA TCVDITATICAVTWIVIDFA* -2.884 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22074 NLL 3 SLAY-screened peptide P424 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCTGCTGTAGCTTTATTAGAATTCGGTTCAGAGCCTTGTTACGGGTTGCCATTGGTTTTAA NLL*LY*NSVQSLVTGCHWF* -2.884 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22075 DWTYTYVSRPIASLADLHAI 20 SLAY-screened peptide P425 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTGGACCTACACTTATGTGTCCCGGCCTATTGCTTCTCTTGCTGACCTCCATGCGATCTAA DWTYTYVSRPIASLADLHAI* -2.879 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22076 MAGSVAYTSSFSNPCTVNHY 20 SLAY-screened peptide P426 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGGCCGGTTCCGTTGCCTATACTTCGTCGTTCTCTAACCCTTGTACTGTCAATCATTATTAA MAGSVAYTSSFSNPCTVNHY* -2.876 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22077 LIFIVLSHSTPHARGPPGRA 20 SLAY-screened peptide P427 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGATTTTTATCGTGCTTTCCCATAGTACGCCGCACGCGAGGGGCCCCCCGGGGCGTGCCTAA LIFIVLSHSTPHARGPPGRA* -2.876 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22078 LLFAFPVPGNVPEVLAENTP 20 SLAY-screened peptide P428 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTTTTTGCTTTCCCTGTCCCTGGTAATGTCCCTGAGGTTCTTGCTGAGAACACTCCCTAA LLFAFPVPGNVPEVLAENTP* -2.867 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22079 DIVSLSRRIPFERTFDPK 18 SLAY-screened peptide P429 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATATTGTGTCCTTGTCGCGTAGGATCCCCTTTGAGCGCACGTTTGACCCCAAGTAGAAGTAA DIVSLSRRIPFERTFDPK*K* -2.866 4.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22080 NFHDETIKLLSPNLYALAIS 20 SLAY-screened peptide P430 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTTCCACGATGAGACTATTAAGTTGCTTAGTCCTAATCTCTACGCGCTGGCTATTAGTTAA NFHDETIKLLSPNLYALAIS* -2.864 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22081 PAVGNYSYVFINSLTAGFLV 20 SLAY-screened peptide P431 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCCGTGGGCAACTATTCTTACGTGTTCATTAACAGCCTTACTGCGGGTTTTCTGGTGTAA PAVGNYSYVFINSLTAGFLV* -2.862 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22082 SRITANNSHIITRETKLCYW 20 SLAY-screened peptide P432 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCGGATTACCGCTAATAATAGCCATATTATTACCCGTGAGACCAAGTTGTGCTACTGGTAA SRITANNSHIITRETKLCYW* -2.862 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22083 HGHASDYIDPHGAQC 15 SLAY-screened peptide P433 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGGGCATGCGTCCGATTACATTGACCCGCATGGGGCCCAGTGTTAGACCAGGTGCCACTAA HGHASDYIDPHGAQC*TRCH* -2.856 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22084 CNSYPVYDHHSHTAYDQFQ 19 SLAY-screened peptide P434 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTAATAGTTACCCTGTGTATGATCATCACAGTCACACGGCTTATGATCAGTTTCAGTAGTAA CNSYPVYDHHSHTAYDQFQ** -2.855 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22085 YGGYSIRFSHYYIYMSSPHL 20 SLAY-screened peptide P435 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGGCGGGTATTCTATCAGGTTCTCCCATTATTATATTTACATGTCCAGTCCGCATTTGTAA YGGYSIRFSHYYIYMSSPHL* -2.854 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22086 CID 3 SLAY-screened peptide P436 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCATTGATTAGTCTAGCGCTCTGCTGCGGCCGAGTATGTAGATGCAGGTTTCTCCCGTTTAA CID*SSALLRPSM*MQVSPV* -2.852 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22087 PSAAVGLIPFLMARANYYLT 20 SLAY-screened peptide P437 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTGCGGCCGTCGGCCTGATCCCCTTTCTTATGGCTAGGGCGAACTACTATCTGACGTAA PSAAVGLIPFLMARANYYLT* -2.851 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22088 TLETRFYLFYTLDTMMSKHN 20 SLAY-screened peptide P438 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTGGAGACTCGCTTTTACCTTTTTTATACTCTTGACACGATGATGTCGAAGCACAACTAA TLETRFYLFYTLDTMMSKHN* -2.851 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22089 CRNCVYHHYNISPNASPASD 20 SLAY-screened peptide P439 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAGGAACTGCGTTTATCACCATTACAATATTAGCCCTAATGCCTCGCCTGCTTCCGATTAA CRNCVYHHYNISPNASPASD* -2.849 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22090 DHPSTCHHGVGPCLFLNYNI 20 SLAY-screened peptide P440 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCACCCTAGTACCTGTCATCATGGCGTTGGCCCGTGCCTCTTTCTCAATTACAATATCTAA DHPSTCHHGVGPCLFLNYNI* -2.846 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22091 IDHCIVGVRNSLARVLANGF 20 SLAY-screened peptide P441 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGATCATTGTATTGTGGGCGTTCGCAATAGCTTGGCGAGGGTTCTCGCGAACGGGTTTTAA IDHCIVGVRNSLARVLANGF* -2.842 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22092 SYNGPSDSPHTHSRHCSFQR 20 SLAY-screened peptide P442 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTATAATGGTCCTTCCGATTCTCCCCATACTCATTCTCGGCATTGTTCGTTCCAGCGCTAA SYNGPSDSPHTHSRHCSFQR* -2.84 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22093 YIGVPGLASRYVLSVLLHGV 20 SLAY-screened peptide P443 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATATCGGTGTTCCGGGTCTGGCCTCCCGTTACGTTCTTTCTGTCTTGCTTCACGGTGTCTAA YIGVPGLASRYVLSVLLHGV* -2.839 2.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22094 VEHPISLRFFFGVRSVCVIN 20 SLAY-screened peptide P444 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGAGCACCCTATTAGCCTGCGCTTTTTTTTCGGTGTGCGCAGCGTTTGCGTTATTAATTAA VEHPISLRFFFGVRSVCVIN* -2.839 1.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22095 WLPPHRDPRL 10 SLAY-screened peptide P445 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCTGCCCCCGCATCGCGACCCCAGGCTTTAGTCCTCCCTGCCTGCTCAGCCGGACGTCTAA WLPPHRDPRL*SSLPAQPDV* -2.839 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22096 EILLLIRIGILILWIMISLGN 21 SLAY-screened peptide P446 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGATACTTCTTCTCATACGCATAGGCATACTAATTTTGTGGATAATGATCTCACTGGGTAAC EILLLIRIGILILWIMISLGN -2.836 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22097 YFTHPHIYAVSPTVTQFFIA 20 SLAY-screened peptide P447 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTTCACTCATCCTCATATTTATGCTGTGTCTCCCACTGTCACCCAGTTTTTTATTGCCTAA YFTHPHIYAVSPTVTQFFIA* -2.835 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22098 FTQAREAPCTPDMSSDH 17 SLAY-screened peptide P448 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTACCCAGGCGCGCGAGGCGCCCTGCACCCCCGACATGTCTAGTGATCATTAGTTTCGCTAA FTQAREAPCTPDMSSDH*FR* -2.834 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22099 CPTVLDYHSRDSTTTFSLEP 20 SLAY-screened peptide P449 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCGACTGTGCTTGACTACCATTCGCGCGATTCTACTACTACGTTTTCGCTCGAGCCCTAA CPTVLDYHSRDSTTTFSLEP* -2.832 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22100 FTPTCCVTRLHTSAQLRVRH 20 SLAY-screened peptide P450 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTACCCCGACTTGCTGCGTCACTCGCCTGCATACTAGCGCTCAGCTTCGCGTTAGGCATTAA FTPTCCVTRLHTSAQLRVRH* -2.831 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22101 RPNWNIRSCIQCEFQIQ 17 SLAY-screened peptide P451 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCCGAATTGGAATATCAGGAGTTGCATCCAGTGCGAGTTTCAGATTCAGTAGTATATGTAA RPNWNIRSCIQCEFQIQ*YM* -2.831 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22102 TAPRVAAPHTLHCNRWWLTP 20 SLAY-screened peptide P452 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGCGCCCCGGGTCGCCGCCCCGCATACGCTTCACTGTAATCGTTGGTGGCTCACCCCTTAA TAPRVAAPHTLHCNRWWLTP* -2.829 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22103 DLYHSYHDCHHNTA 14 SLAY-screened peptide P453 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCTCTATCATAGTTATCACGATTGCCATCATAATACTGCTTAGTTTATGAACACCTATTAA DLYHSYHDCHHNTA*FMNTY* -2.828 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22104 TPRDVDADLGPVATPRTIFM 20 SLAY-screened peptide P454 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCCCCGCGATGTGGATGCTGATCTGGGTCCTGTCGCCACTCCCCGTACCATCTTTATGTAA TPRDVDADLGPVATPRTIFM* -2.826 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22105 LSSNDRPAKYKDSDCGHSYL 20 SLAY-screened peptide P455 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAGCAGCAACGATCGCCCCGCCAAGTACAAGGATAGTGACTGCGGTCATTCCTATTTGTAA LSSNDRPAKYKDSDCGHSYL* -2.826 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22106 TNGHDRKKDTFSCPFISNRH 20 SLAY-screened peptide P456 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAACGGGCACGACCGTAAGAAGGATACGTTTTCGTGTCCTTTTATCAGTAACCGTCATTAA TNGHDRKKDTFSCPFISNRH* -2.825 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22107 NSPFFQNNRYIHAAFDSDLT 20 SLAY-screened peptide P457 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACAGCCCGTTTTTTCAGAATAACCGCTACATTCACGCGGCTTTCGATAGCGACCTCACTTAA NSPFFQNNRYIHAAFDSDLT* -2.824 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22108 SSFRETYYYIPALYFVWGTR 20 SLAY-screened peptide P458 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCTTTCCGGGAGACTTACTATTATATTCCCGCTCTGTACTTTGTTTGGGGTACGCGCTAA SSFRETYYYIPALYFVWGTR* -2.823 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22109 LYVSIILLVGRITFCMTILSN 21 SLAY-screened peptide P459 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTACGTTTCTATCATCTTGCTTGTCGGTCGAATAACCTTCTGCATGACCATACTGTCTAAC LYVSIILLVGRITFCMTILSN -2.819 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22110 FHQKVSGILNRDSINHFDSA 20 SLAY-screened peptide P460 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCATCAGAAGGTGTCCGGGATCCTTAACCGTGATTCTATTAATCATTTCGATTCTGCTTAA FHQKVSGILNRDSINHFDSA* -2.818 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22111 VLSNARSGTFATHGYLLVRY 20 SLAY-screened peptide P461 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCTTAGCAATGCTAGGTCTGGGACTTTTGCTACGCATGGGTACCTTCTTGTGCGCTATTAA VLSNARSGTFATHGYLLVRY* -2.818 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22112 AMSHLLHRQYPHIRSNDPDA 20 SLAY-screened peptide P462 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCATGTCGCATCTCCTTCATCGCCAGTATCCTCATATCCGGAGTAATGATCCGGATGCTTAA AMSHLLHRQYPHIRSNDPDA* -2.814 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22113 YGYCCESPGFQPFGRANGSE 20 SLAY-screened peptide P463 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGGTTACTGTTGCGAGTCCCCGGGGTTCCAGCCTTTCGGCCGCGCTAACGGCTCTGAGTAA YGYCCESPGFQPFGRANGSE* -2.812 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22114 DFVNRLRRFLCNRMHPNAAH 20 SLAY-screened peptide P464 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTTCGTCAATCGGCTGCGTCGGTTTTTGTGCAACCGGATGCACCCCAATGCCGCCCATTAA DFVNRLRRFLCNRMHPNAAH* -2.811 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22115 YLHRPLFSCDLMYVV 15 SLAY-screened peptide P465 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCTTCACCGGCCGCTGTTCTCGTGCGATCTTATGTATGTTGTTTAGCCTTCTCTGCACTAA YLHRPLFSCDLMYVV*PSLH* -2.798 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22116 YMHCSHPCPDLYR 13 SLAY-screened peptide P466 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATATGCATTGTTCTCATCCTTGTCCGGATCTTTACAGGTGACCCTCAATAATATTTGGTAAC YMHCSHPCPDLYR*PSIIFGN -2.798 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22117 TLFTSNQCPYYHHSSTCYRS 20 SLAY-screened peptide P467 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCTTTTTACCTCGAATCAGTGTCCGTATTACCATCACAGTAGTACCTGCTACAGGTCCTAA TLFTSNQCPYYHHSSTCYRS* -2.797 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22118 CPNISTNCRDTDIKKELSTRN 21 SLAY-screened peptide P468 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCTAATATTAGTACTAATTGTCGCGATACTGACATTAAGAAGGAACTTTCGACACGTAAC CPNISTNCRDTDIKKELSTRN -2.796 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22119 TQNYLSDT 8 SLAY-screened peptide P469 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCAGAATTATTTGTCCGATACGTAGAACCGCCCTATCGCGTTCGCTCGCGGTAATCTGTAA TQNYLSDT*NRPIAFARGNL* -2.795 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22120 ARHVFRTNIVLLDIDYSNMS 20 SLAY-screened peptide P470 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCGCCATGTTTTTCGGACTAATATTGTTCTTCTTGATATTGACTATAGCAATATGTCCTAA ARHVFRTNIVLLDIDYSNMS* -2.792 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22121 VVHLVGFTNNRHRDDL 16 SLAY-screened peptide P471 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGTCCACCTGGTTGGGTTTACTAATAACCGTCATCGGGATGATCTCTAGCACCGCTATTAA VVHLVGFTNNRHRDDL*HRY* -2.791 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22122 RDFSWGTPRYWNHMYYNNIL 20 SLAY-screened peptide P472 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGATTTTAGCTGGGGCACTCCGAGGTACTGGAATCATATGTACTATAATAATATCCTTTAA RDFSWGTPRYWNHMYYNNIL* -2.791 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22123 GNRVPATVCPIAISIPLMVD 20 SLAY-screened peptide P473 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTAACCGCGTTCCTGCGACGGTCTGCCCTATTGCTATTTCGATCCCCCTTATGGTCGACTAA GNRVPATVCPIAISIPLMVD* -2.788 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22124 CPSPKCAIVYQTIGPALPRA 20 SLAY-screened peptide P474 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCTAGTCCTAAGTGTGCTATTGTTTACCAGACTATCGGCCCTGCGCTCCCTCGGGCCTAA CPSPKCAIVYQTIGPALPRA* -2.785 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22125 KR 2 SLAY-screened peptide P475 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCGCTAGACTCGTACTGGTCGTTTCATCATCGATCACACTAAGCAGAAGGATAGGTACTAA KR*TRTGRFIIDHTKQKDRY* -2.785 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22126 ITNIVTMQGAHSGFHRDTRT 20 SLAY-screened peptide P476 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCACTAATATTGTTACTATGCAGGGTGCTCACAGTGGGTTCCACCGCGACACGAGGACGTAA ITNIVTMQGAHSGFHRDTRT* -2.783 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22127 GTTSNCDIYANIYTTDLYCG 20 SLAY-screened peptide P477 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCACTACTAGCAATTGTGACATCTACGCGAACATTTACACTACTGACCTTTACTGCGGTTAA GTTSNCDIYANIYTTDLYCG* -2.78 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22128 YQSPSHGYGFPLMNPCYILA 20 SLAY-screened peptide P478 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCAGTCTCCTTCGCATGGTTATGGCTTCCCTTTGATGAACCCTTGCTATATTCTGGCCTAA YQSPSHGYGFPLMNPCYILA* -2.775 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22129 STILSTTI 8 SLAY-screened peptide P479 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTACCATCTTGAGTACTACTATCTAGGCCTCTGCGATTGATTGGACGACGCTCTATCTTTAA STILSTTI*ASAIDWTTLYL* -2.77 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22130 DFLRCLTDLNKDITTLQSLD 20 SLAY-screened peptide P480 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTTCCTCCGTTGCCTCACTGATCTTAACAAGGATATTACTACGCTTCAGAGTCTCGACTAA DFLRCLTDLNKDITTLQSLD* -2.769 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22131 CSYLGFGKFFYL 12 SLAY-screened peptide P481 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTCTTATCTCGGCTTTGGCAAGTTTTTCTATCTCTAGAAGCCTTACCTCTTGCGTGAGTAA CSYLGFGKFFYL*KPYLLRE* -2.763 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22132 ASIHSSGKRPTFTAHRMLVE 20 SLAY-screened peptide P482 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGTCCATTCACAGTTCGGGGAAGCGTCCGACTTTCACCGCTCACAGGATGTTGGTGGAGTAA ASIHSSGKRPTFTAHRMLVE* -2.76 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22133 RQPDWAVLGSVQCPSPNRPF 20 SLAY-screened peptide P483 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCAGCCGGATTGGGCGGTCCTCGGCTCTGTCCAGTGTCCGTCTCCTAATCGTCCTTTTTAA RQPDWAVLGSVQCPSPNRPF* -2.757 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22134 IPTPF 5 SLAY-screened peptide P484 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATACCGACTCCATTTTGAAGAATCTGTGTGACTTCTACAAGCGGACTCTCACCCACTATTAAC IPTPF*RICVTSTSGLSPTIN -2.754 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22135 LEPDFPWCGYNCGNRRRHHS 20 SLAY-screened peptide P485 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGAGCCGGACTTCCCCTGGTGTGGCTACAACTGTGGTAACCGTAGGCGGCATCATTCTTAA LEPDFPWCGYNCGNRRRHHS* -2.753 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22136 TAPITLIRGPPSGHGYSACH 20 SLAY-screened peptide P486 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGCCCCTATCACTCTCATCCGCGGCCCGCCGAGCGGGCATGGTTACAGTGCGTGTCATTAA TAPITLIRGPPSGHGYSACH* -2.752 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22137 SPIIKLYNEDVAHYDDLNI 19 SLAY-screened peptide P487 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCCGATTATTAAGCTCTACAATGAGGATGTTGCTCATTATGACGACCTTAACATCTAGTAA SPIIKLYNEDVAHYDDLNI** -2.751 3.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22138 PPLFSGSGCNNHADYRSTSS 20 SLAY-screened peptide P488 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTCTTTTTAGCGGTTCTGGTTGCAATAACCATGCTGACTATCGGTCCACTAGTTCCTAA PPLFSGSGCNNHADYRSTSS* -2.749 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22139 TDSMDFRSFDDAVGDIVYSA 20 SLAY-screened peptide P489 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGATAGTATGGACTTCCGTAGCTTTGACGACGCCGTCGGGGATATCGTTTACTCTGCGTAA TDSMDFRSFDDAVGDIVYSA* -2.748 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22140 PATATGRCIGPVPYSTSDNL 20 SLAY-screened peptide P490 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCGACTGCCACGGGGCGCTGTATCGGCCCCGTGCCTTATTCTACCTCCGACAATCTTTAA PATATGRCIGPVPYSTSDNL* -2.747 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22141 DIPNLRVYYYDRHFTLIYMK 20 SLAY-screened peptide P491 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATATCCCTAACTTGCGCGTCTACTATTACGACCGTCACTTTACGCTTATTTATATGAAGTAA DIPNLRVYYYDRHFTLIYMK* -2.745 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22142 RNNNNSHAIHCSNTRDLGAC 20 SLAY-screened peptide P492 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAATAACAATAACAGCCATGCTATCCATTGTAGCAATACGAGGGATTTGGGTGCCTGTTAA RNNNNSHAIHCSNTRDLGAC* -2.744 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22143 GLVIRTGGTLTFSSIIPTTK 20 SLAY-screened peptide P493 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCTGGTCATTCGTACTGGCGGTACGCTCACTTTTAGTAGTATCATCCCTACGACCAAGTAA GLVIRTGGTLTFSSIIPTTK* -2.744 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22144 HPSTWTMFTIDKSTLSFWTT 20 SLAY-screened peptide P494 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCTAGTACTTGGACTATGTTTACTATTGACAAGAGCACGCTCTCCTTCTGGACCACTTAA HPSTWTMFTIDKSTLSFWTT* -2.741 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22145 TRSTLLCCRSILML 14 SLAY-screened peptide P495 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCGATCAACATTGCTTTGCTGCCGTTCTATATTAATGCTTTGAGAAGCGGTTTCATTAACT TRSTLLCCRSILML*EAVSLT -2.74 6.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22146 HCSTRLITLATPPTSQFFNS 20 SLAY-screened peptide P496 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTGTAGTACGCGTCTCATCACTCTTGCTACCCCTCCTACGTCTCAGTTTTTTAATAGTTAA HCSTRLITLATPPTSQFFNS* -2.734 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22147 LRVSEESGSSCRIAGILRGMS 21 SLAY-screened peptide P497 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCGGGTATCGGAGGAGAGCGGTTCTTCCTGTCGTATTGCGGGTATACTTCGCGGGATGTCT LRVSEESGSSCRIAGILRGMS -2.732 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22148 FSLYIRTYTNYKTNIILYII 20 SLAY-screened peptide P498 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTCGCTGTATATCCGCACTTATACTAACTATAAGACTAATATTATTCTCTATATCATTTAA FSLYIRTYTNYKTNIILYII* -2.732 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22149 VPMGCSPAPWYNHFGKRSYM 20 SLAY-screened peptide P499 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCCTATGGGCTGTAGCCCTGCTCCTTGGTACAATCATTTCGGTAAGCGCAGCTATATGTAA VPMGCSPAPWYNHFGKRSYM* -2.731 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22150 RPCFTSVFNSPFFFHNTQQF 20 SLAY-screened peptide P500 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCCTTGCTTCACTTCGGTCTTTAACAGCCCTTTTTTTTTTCATAACACGCAGCAGTTCTAA RPCFTSVFNSPFFFHNTQQF* -2.731 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22151 CPSARCYFCRSVNITDCNTH 20 SLAY-screened peptide P501 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCTAGTGCTCGTTGTTACTTCTGTCGTAGTGTTAATATTACGGATTGTAATACTCACTAA CPSARCYFCRSVNITDCNTH* -2.73 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22152 HPHPITFDFRRPHTLVPPPS 20 SLAY-screened peptide P502 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCCCCACCCTATCACTTTTGATTTTCGTCGCCCCCATACTTTGGTCCCTCCGCCTTCGTAA HPHPITFDFRRPHTLVPPPS* -2.729 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22153 TPVYTYNLISTWPARETVYL 20 SLAY-screened peptide P503 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCCCGTTTATACGTACAATCTCATTTCCACCTGGCCGGCGCGCGAGACTGTCTACTTGTAA TPVYTYNLISTWPARETVYL* -2.727 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22154 QVTHGLPMAII 11 SLAY-screened peptide P504 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGTCACTCATGGGTTGCCTATGGCCATTATTTAGTTGCATCCTGCCGACGGTGCTGTCTAA QVTHGLPMAII*LHPADGAV* -2.727 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22155 PLVLDITWEYALARHNNNLL 20 SLAY-screened peptide P505 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTTGGTCCTGGACATTACTTGGGAGTACGCTTTGGCCCGTCACAACAATAATTTGCTTTAA PLVLDITWEYALARHNNNLL* -2.72 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22156 LRRGDSHFSLVNFYNNTAYY 20 SLAY-screened peptide P506 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCGCAGGGGCGACAGCCATTTCAGCCTTGTCAATTTCTATAATAATACGGCTTACTATTAA LRRGDSHFSLVNFYNNTAYY* -2.715 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22157 ILCRHVFPRPCDTTYSSDRD 20 SLAY-screened peptide P507 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTTTGTGCCGCCACGTTTTTCCTCGGCCCTGCGACACCACTTATAGTTCTGATAGGGATTAA ILCRHVFPRPCDTTYSSDRD* -2.715 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22158 RIC 3 SLAY-screened peptide P508 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGATTTGTTAGCTGCCCGTTAACTATAACGAGCGGACCGATGTCCCGAAGGTCGCTTCCTAA RIC*LPVNYNERTDVPKVAS* -2.712 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22159 CRHLPGVELVKISL 14 SLAY-screened peptide P509 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGCCACCTTCCTGGCGTGGAGCTTGTTAAGATTTCTTTGTAGTTCAGTACTTATGCGTAA CRHLPGVELVKISL*FSTYA* -2.712 5.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22160 HRLGTLRSALLLFCIYVLVR 20 SLAY-screened peptide P510 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCGCTTGGGCACTCTGCGTTCTGCGCTTCTTCTTTTCTGCATCTATGTCCTTGTTCGTTAA HRLGTLRSALLLFCIYVLVR* -2.711 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22161 PG 2 SLAY-screened peptide P511 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGGGTAGTACAGCTAATCCCCTGTGCCTATGTGAGTTTCACGAAGCGTTTTCATGCCTAAC PG*YS*SPVPM*VSRSVFMPN -2.711 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22162 FNPSLNWTNFVRPVTMVSQT 20 SLAY-screened peptide P512 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTAACCCGTCGCTTAACTGGACTAATTTTGTGCGGCCTGTTACCATGGTTAGCCAGACCTAA FNPSLNWTNFVRPVTMVSQT* -2.71 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22163 RPMRLKAYQPHHPPHYRWIE 20 SLAY-screened peptide P513 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCCATGCGCCTCAAGGCTTATCAGCCTCATCATCCTCCGCATTATCGTTGGATTGAGTAA RPMRLKAYQPHHPPHYRWIE* -2.708 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22164 YHDCAPPQLSLGDLYTLIAS 20 SLAY-screened peptide P514 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCACGACTGTGCTCCTCCCCAGTTGAGTCTTGGTGATCTTTATACGCTTATCGCGTCCTAA YHDCAPPQLSLGDLYTLIAS* -2.706 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22165 NPSLRIYYDNSSCRYKPWLN 20 SLAY-screened peptide P515 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCCTTCTCTTCGTATCTATTACGACAATAGTAGTTGTCGCTATAAGCCCTGGCTCAACTAA NPSLRIYYDNSSCRYKPWLN* -2.703 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22166 VFFWSLVLVRRLSKRQP 17 SLAY-screened peptide P516 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTTTTTTTGGTCGCTGGTCCTTGTTAGGAGGCTTTCGAAGCGTCAGCCCTAGACTCCCTAA VFFWSLVLVRRLSKRQP*TP* -2.702 0.03098 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22167 GASHLRAYPYIRNVTSFTLY 20 SLAY-screened peptide P517 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGGCGTCTCATCTCCGTGCTTATCCTTATATTCGGAATGTCACGTCTTTTACTCTTTATTAA GASHLRAYPYIRNVTSFTLY* -2.699 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22168 STLLTRNLYDYRWQCCAWSI 20 SLAY-screened peptide P518 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCACTCTGCTTACTAGGAATTTGTATGACTATCGTTGGCAGTGTTGCGCTTGGTCTATTTAA STLLTRNLYDYRWQCCAWSI* -2.697 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22169 TGLSNDHGDYYSQSKCGEVGY 21 SLAY-screened peptide P519 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGTTTGTCCAATGACCATGGGGACTACTACTCTCAGTCTAAGTGTGGGGAGGTGGGCTAC TGLSNDHGDYYSQSKCGEVGY -2.697 3.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22170 NDKSNSYPSFDLFCDSVALP 20 SLAY-screened peptide P520 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGATAAGAGCAATTCCTACCCTAGTTTTGACTTGTTTTGCGATTCCGTGGCGCTCCCCTAA NDKSNSYPSFDLFCDSVALP* -2.694 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22171 DCLYLYALPASLHCYLIRHA 20 SLAY-screened peptide P521 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTGCCTGTACCTTTACGCGCTCCCTGCTTCGTTGCACTGTTACCTTATTCGTCACGCCTAA DCLYLYALPASLHCYLIRHA* -2.694 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22172 YRFNLGFLYVNDQLCTRTDR 20 SLAY-screened peptide P522 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCGCTTTAATCTCGGCTTCCTTTATGTCAACGATCAGCTTTGTACTCGTACTGACCGCTAA YRFNLGFLYVNDQLCTRTDR* -2.694 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22173 ILRYHRCTDHKQRRHGRRPI 20 SLAY-screened peptide P523 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCTTCGGTATCATCGTTGTACTGATCATAAGCAGCGGCGTCATGGGAGGCGTCCCATTTAA ILRYHRCTDHKQRRHGRRPI* -2.693 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22174 TLNATVNSCSVNCL 14 SLAY-screened peptide P524 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTCAACGCCACTGTTAATAGTTGTAGTGTCAATTGTCTCTAGGCCGCTCTGCGTACGTAA TLNATVNSCSVNCL*AALRT* -2.693 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22175 AAGRSITYSWAVLRLCPHWF 20 SLAY-screened peptide P525 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGCCGGTCGCAGCATCACCTACAGTTGGGCTGTGCTCAGGCTTTGCCCCCACTGGTTCTAA AAGRSITYSWAVLRLCPHWF* -2.692 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22176 LTSLFLKDSPYNSSNAPELT 20 SLAY-screened peptide P526 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACTAGCCTGTTCCTCAAGGACAGCCCTTATAATAGTTCTAATGCGCCTGAGTTGACCTAA LTSLFLKDSPYNSSNAPELT* -2.69 3.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22177 YTT 3 SLAY-screened peptide P527 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATACTACTTAGATGCGTACCACGTCGATTTACAATAATTGCTTTACCGCTCTTGTGCCCTAA YTT*MRTTSIYNNCFTALVP* -2.689 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22178 PPGVLAHVHLGLASSART 18 SLAY-screened peptide P528 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCCGGGGTGCTGGCCCATGTGCATCTGGGCCTCGCCTCGTCCGCTAGGACCTAGCAGTAA PPGVLAHVHLGLASSART*Q* -2.689 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22179 SSKQFQ 6 SLAY-screened peptide P529 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTCTAAGCAGTTCCAGTAGGACTCGTGCCCGAGCGGCCCCCAGCACACCTTGACGGCTTAA SSKQFQ*DSCPSGPQHTLTA* -2.689 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22180 TLG 3 SLAY-screened peptide P530 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTGGGGTAGGAGAGGTGGGCGACGGGCCAGCTCGCTCCCAACTCCAGGGACAACGTGTAA TLG*ERWATGQLAPNSRDNV* -2.686 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22181 SHRVLAPTAQLKFTLCYPGA 20 SLAY-screened peptide P531 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCATCGCGTCTTGGCGCCGACCGCCCAGCTCAAGTTCACTCTGTGTTACCCTGGTGCCTAA SHRVLAPTAQLKFTLCYPGA* -2.684 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22182 NTPGRPKPTHYMCSLLPAIA 20 SLAY-screened peptide P532 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACACTCCTGGCCGGCCGAAGCCTACCCATTATATGTGTAGTTTGCTTCCGGCTATCGCTTAA NTPGRPKPTHYMCSLLPAIA* -2.681 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22183 NRMVVTINKTTTHHV 15 SLAY-screened peptide P533 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCGTATGGTTGTGACGATCAACAAGACGACTACTCATCACGTCTAGGACGGTCTTTATTAA NRMVVTINKTTTHHV*DGLY* -2.68 2.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22184 LSAAPRVRVATDYNGSLPNP 20 SLAY-screened peptide P534 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTCCGCTGCTCCGAGGGTCCGTGTGGCGACTGATTATAACGGTAGCCTTCCCAACCCCTAA LSAAPRVRVATDYNGSLPNP* -2.68 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22185 GRYSHQMRPTSYSANSLI 18 SLAY-screened peptide P535 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCGCTATTCCCACCAGATGCGGCCTACTTCTTATTCGGCTAATTCGCTCATCTAGGGCTAA GRYSHQMRPTSYSANSLI*G* -2.679 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22186 TVSKDDALSLDPSSILKEMP 20 SLAY-screened peptide P536 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGTGTCTAAGGACGATGCTCTTTCGCTGGATCCTAGTAGCATTCTTAAGGAGATGCCCTAA TVSKDDALSLDPSSILKEMP* -2.679 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22187 PIHDYVRLSFISSCCTCAFN 20 SLAY-screened peptide P537 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCATCCACGACTATGTGCGTCTTAGTTTCATCAGTAGTTGTTGCACTTGTGCGTTTAATTAA PIHDYVRLSFISSCCTCAFN* -2.676 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22188 LRPTSYSG 8 SLAY-screened peptide P538 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAGGCCCACTAGCTACAGCGGCTAGTTTTACAATTATTATACGCTCTCGAAGTATCTGTAA LRPTSYSG*FYNYYTLSKYL* -2.676 0.000314 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22189 TRLNRPTYTHTSRDTDFDFL 20 SLAY-screened peptide P539 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCGGCTTAACCGTCCGACGTACACGCACACTTCGCGCGACACTGACTTTGATTTCCTTTAA TRLNRPTYTHTSRDTDFDFL* -2.674 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22190 QCHPLWLWHARDPPSPCRKQ 20 SLAY-screened peptide P540 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTGCCATCCTTTGTGGCTTTGGCACGCGCGGGACCCGCCGTCTCCGTGCAGGAAGCAGTAA QCHPLWLWHARDPPSPCRKQ* -2.666 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22191 TD 2 SLAY-screened peptide P541 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGATTAGAACTACGGTTCGGCGTATGGCTATTCTCCGAGTTTTATGAATTGTGAGTCGTAA TD*NYGSAYGYSPSFMNCES* -2.666 0.000104 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22192 ARSPVTKAIHSRANYNVFPN 20 SLAY-screened peptide P542 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCGTAGCCCTGTTACCAAGGCCATTCATTCTCGTGCTAATTATAATGTGTTCCCGAACTAA ARSPVTKAIHSRANYNVFPN* -2.659 1.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22193 APYTH 5 SLAY-screened peptide P543 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCCTTATACTCATTAGAATACGGATAACGACTGGAATCCGAGTATCAAGCAGAGTCCGTAA APYTH*NTDNDWNPSIKQSP* -2.656 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22194 PTDSTVHVGDIGLFYENTSF 20 SLAY-screened peptide P544 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACTGACTCTACTGTCCACGTCGGTGACATTGGTTTGTTTTATGAGAATACTAGCTTCTAA PTDSTVHVGDIGLFYENTSF* -2.654 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22195 RTHFTSGFGRHCNIVCTFHF 20 SLAY-screened peptide P545 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGACGCATTTCACGTCGGGCTTTGGCAGGCACTGCAATATCGTCTGCACCTTCCACTTCTAA RTHFTSGFGRHCNIVCTFHF* -2.653 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22196 TTYCLRSPNAPSPNIH 16 SLAY-screened peptide P546 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGACGTATTGCCTCCGGTCTCCGAATGCTCCTTCGCCGAATATTCATTAGCATCGCCTTTAA TTYCLRSPNAPSPNIH*HRL* -2.653 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22197 LSRNFIIRKGLNVRPQILLC 20 SLAY-screened peptide P547 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTCTCGCAATTTCATTATCCGCAAGGGCCTTAACGTGCGTCCTCAGATCCTTCTCTGCTAA LSRNFIIRKGLNVRPQILLC* -2.652 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22198 PPTEQPSIPTARASPTPQDS 20 SLAY-screened peptide P548 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCCACTGAGCAGCCTTCTATCCCCACCGCCCGTGCTTCCCCGACGCCGCAGGATAGCTAA PPTEQPSIPTARASPTPQDS* -2.651 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22199 LHNADHRAADHRTISRHK 18 SLAY-screened peptide P549 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACAACGCCGATCACCGCGCGGCTGATCATCGCACTATTAGTCGTCACAAGTAATGAGTA LHNADHRAADHRTISRHK**V -2.647 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22200 FKTCFYRDPTIACSHHCDTD 20 SLAY-screened peptide P550 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCAAGACTTGTTTTTATCGTGACCCTACGATTGCGTGTTCTCACCATTGTGACACCGACTAA FKTCFYRDPTIACSHHCDTD* -2.646 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22201 VLLNRYFKEIGILSRFGTSL 20 SLAY-screened peptide P551 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCTCCTCAACCGCTATTTCAAGGAGATCGGTATCCTTAGTCGTTTCGGTACGAGTCTGTAA VLLNRYFKEIGILSRFGTSL* -2.642 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22202 ATLCSNTIGVHD 12 SLAY-screened peptide P552 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCACTCTCTGCTCTAACACGATCGGGGTTCATGATTAGAATATGTACTAGCATATCTATTAA ATLCSNTIGVHD*NMY*HIY* -2.642 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22203 DTTLPSSNGVESPNRNIAIS 20 SLAY-screened peptide P553 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATACTACCCTCCCTTCGTCTAACGGGGTTGAGAGTCCTAATCGCAATATTGCTATTAGCTAA DTTLPSSNGVESPNRNIAIS* -2.641 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22204 ESDHSPSHLVLHSTHYLTHF 20 SLAY-screened peptide P554 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGAGTGATCATTCGCCTTCTCATCTTGTTCTTCATTCTACGCACTACCTGACGCATTTCTAA ESDHSPSHLVLHSTHYLTHF* -2.64 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22205 RSCHAQHCYWYYTLLRASIP 20 SLAY-screened peptide P555 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTCCTGCCATGCTCAGCATTGTTATTGGTATTATACTTTGCTGCGTGCCTCCATTCCTTAA RSCHAQHCYWYYTLLRASIP* -2.638 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22206 WGGHSMLCLLLAPRGAFAAV 20 SLAY-screened peptide P556 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGGGTGGCCATAGTATGCTCTGTCTTCTTCTCGCCCCTCGCGGTGCGTTTGCTGCTGTCTAA WGGHSMLCLLLAPRGAFAAV* -2.635 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22207 LIHHSHVLTDSCFFHRNGIE 20 SLAY-screened peptide P557 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCATCCATCACTCTCATGTCCTTACTGATAGCTGCTTTTTCCATCGCAATGGTATTGAGTAA LIHHSHVLTDSCFFHRNGIE* -2.635 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22208 CLHTPYPQNLCLNRVCNNLS 20 SLAY-screened peptide P558 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCTGCATACCCCTTATCCCCAGAATCTTTGCCTCAATCGTGTTTGTAATAATCTTAGCTAA CLHTPYPQNLCLNRVCNNLS* -2.632 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22209 VHIPLHSP 8 SLAY-screened peptide P559 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCACATTCCCCTCCACAGCCCCTAGAATTGCATTTACTACTACAGTGGAAGTTTTTTTAAC VHIPLHSP*NCIYYYSGSFFN -2.628 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22210 CEFLDALGHCHSLSGFPGNV 20 SLAY-screened peptide P560 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGAGTTCCTGGACGCTCTGGGCCATTGTCACTCGCTCTCGGGCTTTCCCGGTAATGTGTAA CEFLDALGHCHSLSGFPGNV* -2.628 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22211 YDHFPNFNDNYCWPPITCYL 20 SLAY-screened peptide P561 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGATCATTTTCCCAATTTCAATGACAACTATTGCTGGCCTCCTATTACTTGCTATCTGTAA YDHFPNFNDNYCWPPITCYL* -2.627 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22212 SASFT 5 SLAY-screened peptide P562 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGCTAGTTTTACGTAGATGCTGTCGGTTTGTGTTGAGGCGCGTACGCCCGCTATTAGGTAA SASFT*MLSVCVEARTPAIR* -2.627 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22213 LPSSMPHILFHCWVGLNRSN 20 SLAY-screened peptide P563 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCCTCGAGCATGCCTCACATCCTTTTTCATTGTTGGGTTGGCCTTAATAGGAGTAACTAA LPSSMPHILFHCWVGLNRSN* -2.625 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22214 ARWSFLFLLSTAHCPLPNRN 20 SLAY-screened peptide P564 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCGGTGGTCGTTCTTGTTTCTGTTGTCCACTGCGCACTGTCCCTTGCCGAATCGGAATTAA ARWSFLFLLSTAHCPLPNRN* -2.624 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22215 LPPY 4 SLAY-screened peptide P565 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCCCCTTATTAGGTTCTTTACCATTTCCATGCTCCTCGGGAGATTGAGAAGATGATCTAA LPPY*VLYHFHAPREIEKMI* -2.623 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22216 LYVRAAYPNLSWSVPVLRVP 20 SLAY-screened peptide P566 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTACGTCCGCGCTGCCTATCCGAATTTGTCCTGGTCTGTGCCTGTTCTTCGCGTTCCGTAA LYVRAAYPNLSWSVPVLRVP* -2.617 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22217 PCTRSTLSPPNFVVELVSNW 20 SLAY-screened peptide P567 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGTACTCGCTCGACCCTTAGTCCGCCCAATTTTGTGGTTGAGCTTGTTAGTAATTGGTAA PCTRSTLSPPNFVVELVSNW* -2.617 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22218 PDGFLRRSIPVNPSQTHFAH 20 SLAY-screened peptide P568 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGACGGTTTTCTTAGGCGTAGTATCCCTGTGAACCCGTCCCAGACGCATTTCGCTCATTAA PDGFLRRSIPVNPSQTHFAH* -2.617 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22219 PKRRRHFSNQLLRAVGKFDD 20 SLAY-screened peptide P569 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAAGCGCCGTCGTCATTTTAGCAATCAGCTTCTTCGCGCGGTGGGCAAGTTCGATGACTAA PKRRRHFSNQLLRAVGKFDD* -2.616 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22220 DNNNHLK 7 SLAY-screened peptide P570 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACAATAACAATCATCTTAAGTAGCTGTACTTGAACAAGGGTACGTTCTCCGCCGGCATCTAC DNNNHLK*LYLNKGTFSAGIY -2.616 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22221 LAVVASSCYTIHSHTNPPVT 20 SLAY-screened peptide P571 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGGCCGTTGTGGCTAGCAGCTGCTATACGATCCATTCCCATACTAATCCCCCTGTTACTTAA LAVVASSCYTIHSHTNPPVT* -2.612 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22222 LHFSRLRRL 9 SLAY-screened peptide P572 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACTTCTCTAGATTAAGGCGCTTATGACCATGTCCAATACCTACTGCGGGTTGTCCTAAC LHFSRLRRL*PCPIPTAGCPN -2.605 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22223 VTSEPCASAPRGPHPLADSS 20 SLAY-screened peptide P573 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGACCAGCGAGCCGTGCGCCTCTGCGCCGCGGGGCCCGCACCCTCTCGCGGACTCCAGCTAA VTSEPCASAPRGPHPLADSS* -2.603 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22224 TARNPGPLLSHALCFLANTV 20 SLAY-screened peptide P574 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGCGCGGAACCCCGGTCCGCTGCTTTCTCACGCTTTGTGCTTTCTGGCCAATACCGTCTAA TARNPGPLLSHALCFLANTV* -2.603 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22225 QLRRENTETILSSIRILPLAN 21 SLAY-screened peptide P575 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCTCCGGCGTGAGAATACTGAGACCATCTTGTCCTCTATACGAATATTGCCCCTTGCTAAC QLRRENTETILSSIRILPLAN -2.602 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22226 VNFNPCPHSNISRPT 15 SLAY-screened peptide P576 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTAATTTTAATCCCTGTCCTCATTCGAATATTTCGCGCCCTACTTAGTGCTGCATTCCCTAA VNFNPCPHSNISRPT*CCIP* -2.602 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22227 SLSVPHLRHMLLATTTLALR 20 SLAY-screened peptide P577 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTTGTCTGTGCCTCACCTCCGGCATATGCTGCTCGCTACCACCACCCTCGCTCTGCGCTAA SLSVPHLRHMLLATTTLALR* -2.601 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22228 FYGVLIYLRYLFFSFAIFVF 20 SLAY-screened peptide P578 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTATGGTGTGCTTATCTACCTGCGCTACCTCTTCTTTTCCTTCGCGATCTTCGTTTTTTAA FYGVLIYLRYLFFSFAIFVF* -2.598 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22229 TGPLRHFVNYYNIAHDQTTT 20 SLAY-screened peptide P579 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGCCCGCTCCGGCACTTCGTTAATTACTACAATATCGCTCATGATCAGACTACCACTTAA TGPLRHFVNYYNIAHDQTTT* -2.597 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22230 RRTRTPRGDAKNSAYSLGAP 20 SLAY-screened peptide P580 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTAGGACTCGGACCCCGCGGGGTGATGCCAAGAATTCTGCTTATTCTCTGGGTGCTCCCTAA RRTRTPRGDAKNSAYSLGAP* -2.595 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22231 DLRQDLSGLRIFTISTADLCN 21 SLAY-screened peptide P581 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCTTCGGCAGGACCTCTCCGGTCTTCGCATTTTTACTATTAGCACTGCGGACTTATGTAAC DLRQDLSGLRIFTISTADLCN -2.594 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22232 LHHMADNNQVGDTLAEMLVS 20 SLAY-screened peptide P582 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCATCACATGGCCGACAACAATCAGGTGGGCGACACTCTTGCTGAGATGCTCGTGAGTTAA LHHMADNNQVGDTLAEMLVS* -2.594 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22233 HNHCHTLYNTPTILRPCACT 20 SLAY-screened peptide P583 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAATCACTGTCACACTCTGTATAATACTCCCACCATCTTGAGGCCGTGTGCCTGTACTTAA HNHCHTLYNTPTILRPCACT* -2.593 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22234 TFLSPLAGPLCNSSYFLASV 20 SLAY-screened peptide P584 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTTTCTGTCCCCTCTCGCCGGGCCCCTCTGCAACTCGTCCTATTTCCTGGCTTCTGTCTAA TFLSPLAGPLCNSSYFLASV* -2.593 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22235 SSIRTSVMDLVNYSTNFRNA 20 SLAY-screened peptide P585 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGAGTATCCGGACCAGTGTTATGGACCTTGTTAATTACTCGACTAACTTTCGTAATGCTTAA SSIRTSVMDLVNYSTNFRNA* -2.592 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22236 LFPAQPAGCHFISPLLPVPAN 21 SLAY-screened peptide P586 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTTTCCTGCTCAGCCTGCTGGCTGCCATTTCATCAGTCCATTATTACCAGTCCCCGCTAAC LFPAQPAGCHFISPLLPVPAN -2.592 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22237 AVARWLRDLNAVDIADFSRS 20 SLAY-screened peptide P587 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGTGGCTCGTTGGCTTCGTGACCTCAACGCTGTTGATATTGCTGACTTCTCTAGGAGCTAA AVARWLRDLNAVDIADFSRS* -2.591 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22238 CLHTTLYTWILGSTFCGFLC 20 SLAY-screened peptide P588 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTGCACACTACCCTCTATACTTGGATTCTGGGTTCGACTTTCTGCGGTTTCCTTTGCTAA CLHTTLYTWILGSTFCGFLC* -2.591 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22239 HLHRRCRQSFFYPRLAPNRM 20 SLAY-screened peptide P589 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTTGCATCGGAGGTGCCGGCAGTCTTTTTTTTATCCTCGTTTGGCTCCCAATCGGATGTAA HLHRRCRQSFFYPRLAPNRM* -2.588 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22240 PTYLALATQHPNDNLGHDRR 20 SLAY-screened peptide P590 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACCTATTTGGCGTTGGCCACTCAGCACCCTAACGACAACCTGGGTCACGATCGTAGGTAA PTYLALATQHPNDNLGHDRR* -2.588 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22241 SVLFRVTTHTHHNKT 15 SLAY-screened peptide P591 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGTCCTTTTTCGTGTCACTACGCATACGCACCATAACAAGACCTAGGGGTAGCCGACCTAA SVLFRVTTHTHHNKT*G*PT* -2.587 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22242 LDSTHHYNKYMYASPL 16 SLAY-screened peptide P592 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGATAGTACGCACCATTATAATAAGTATATGTATGCTTCTCCCTTGTAGTCTAACAATTAA LDSTHHYNKYMYASPL*SNN* -2.586 7.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22243 TPLNYSVRNPHFTIDVPYTS 20 SLAY-screened peptide P593 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCGCTTAACTACTCGGTCCGCAATCCTCATTTTACTATTGACGTTCCCTATACTTCTTAA TPLNYSVRNPHFTIDVPYTS* -2.585 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22244 IIFCAMRPTSHIEPVTTSGN 20 SLAY-screened peptide P594 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTATTTTTTGCGCTATGCGGCCTACTTCTCATATCGAGCCCGTGACTACCTCGGGCAACTAA IIFCAMRPTSHIEPVTTSGN* -2.582 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22245 ASPPVMSSKTPSCVSNITNY 20 SLAY-screened peptide P595 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGTCGCCCCCCGTGATGTCCAGCAAGACTCCTTCTTGTGTCTCTAACATCACTAACTATTAA ASPPVMSSKTPSCVSNITNY* -2.581 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22246 RIVNMSRPPYRIKFSVHSCD 20 SLAY-screened peptide P596 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTATCGTCAATATGTCCCGGCCTCCTTACCGTATTAAGTTTTCTGTGCACTCGTGTGATTAA RIVNMSRPPYRIKFSVHSCD* -2.581 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22247 INLVCI 6 SLAY-screened peptide P597 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAACCTTGTGTGCATTTAGCTTCTCACCGCTGCGCACATTTATTATGTTCGGACGTTTTAA INLVCI*LLTAAHIYYVRTF* -2.579 8.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22248 PSRSMHHYPSRAVLMPLLRVN 21 SLAY-screened peptide P598 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGTCGGTCTATGCATCACTATCCGTCTCGCGCCGTGTTGATGCCGCTGCTGCGAGTTAAC PSRSMHHYPSRAVLMPLLRVN -2.578 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22249 HRKFTSNHLNYCITNKARLLN 21 SLAY-screened peptide P599 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCGGAAGTTCACGTCTAATCATCTTAACTATTGCATTACGAATAAAGCTCGCTTGCTTAAC HRKFTSNHLNYCITNKARLLN -2.577 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22250 RTSIHLFT 8 SLAY-screened peptide P600 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTACTTCGATCCATCTGTTTACCTAGATGTTTAGCAGTGGTTTGGCTTCACTGTGTATTAAC RTSIHLFT*MFSSGLASLCIN -2.576 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22251 SAPYTLVPLSYLNCNLPTDL 20 SLAY-screened peptide P601 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGCCCCTTACACCCTGGTCCCGCTTTCGTATCTTAATTGCAATCTGCCTACTGACTTGTAA SAPYTLVPLSYLNCNLPTDL* -2.575 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22252 TPVYPLSDL 9 SLAY-screened peptide P602 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCTGTTTACCCTTTGTCTGATCTTTAGCCCTTCATGCGCCCCTCGCGCATGAGGTGTTAA TPVYPLSDL*PFMRPSRMRC* -2.571 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22253 LILCVLGCPCFVL 13 SLAY-screened peptide P603 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGATCCTTTGTGTCCTGGGCTGTCCTTGTTTTGTCCTTTAGTCTACATTTTGAGGCCGTAAC LILCVLGCPCFVL*STF*GRN -2.57 0.000586 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22254 PNVCDALPPSRACTLSAPSR 20 SLAY-screened peptide P604 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAATGTCTGTGATGCCCTTCCTCCTTCGCGCGCGTGTACCCTGAGCGCCCCCTCTCGGTAA PNVCDALPPSRACTLSAPSR* -2.57 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22255 PPSVLIDGYFRPSDCSSERTN 21 SLAY-screened peptide P605 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCTTCTGTCCTTATTGACGGCTACTTCAGGCCTTCTGACTGTAGTTCCGAGAGGACTAAC PPSVLIDGYFRPSDCSSERTN -2.57 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22256 PH 2 SLAY-screened peptide P606 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCACTAGCGTTGGCGGCTTCTCTTCCACTATTAGGCCCCCCTTTTGTGCATCCGTTGCTAA PH*RWRLLFHY*APLLCIRC* -2.569 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22257 LSYHLNYPVNCSDHLHGLPC 20 SLAY-screened peptide P607 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTCCTATCATCTCAATTATCCCGTTAATTGTAGTGACCACCTGCACGGTTTGCCCTGTTAA LSYHLNYPVNCSDHLHGLPC* -2.567 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22258 PNGTLIFCPLHSPRFLA 17 SLAY-screened peptide P608 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAATGGGACTCTTATCTTCTGTCCTCTGCATTCTCCTCGCTTTCTCGCTTAGGTGTGTTAA PNGTLIFCPLHSPRFLA*VC* -2.563 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22259 LAAP 4 SLAY-screened peptide P609 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGCTGCCCCTTAGTTTTCTTCTCTTGGTTCCGTGCTTATCCCGACGATCAGCTACAGCTAA LAAP*FSSLGSVLIPTISYS* -2.563 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22260 NLSLNSQGMARHRTDRATLS 20 SLAY-screened peptide P610 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCTGTCGCTCAATTCGCAGGGCATGGCGAGGCACCGCACGGATCGTGCTACTCTGTCGTAA NLSLNSQGMARHRTDRATLS* -2.563 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22261 PRSYNLGTVPPRSDPYNILN 20 SLAY-screened peptide P611 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGTTCCTACAATCTGGGTACGGTGCCTCCGCGTTCTGATCCCTACAATATTCTTAATTAA PRSYNLGTVPPRSDPYNILN* -2.56 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22262 LRLRSLCLFCTVFSNNDSHA 20 SLAY-screened peptide P612 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCGCCTTCGTTCTCTTTGTTTGTTTTGCACGGTTTTCTCCAATAACGATTCCCACGCGTAA LRLRSLCLFCTVFSNNDSHA* -2.559 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22263 NFHSRSPTNLAKTNKNPVME 20 SLAY-screened peptide P613 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTTTCATAGTCGTTCGCCGACTAATCTTGCTAAGACTAATAAGAATCCTGTCATGGAGTAA NFHSRSPTNLAKTNKNPVME* -2.557 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22264 SATNIRSHYLNFMVSILCMT 20 SLAY-screened peptide P614 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGCGACGAATATTAGGAGTCACTATCTCAACTTTATGGTTAGTATTCTGTGCATGACTTAA SATNIRSHYLNFMVSILCMT* -2.556 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22265 PAQVASFILRVFRHIREHMH 20 SLAY-screened peptide P615 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCTCAGGTCGCCAGTTTCATCTTGCGCGTCTTTCGGCATATCCGGGAGCATATGCACTAA PAQVASFILRVFRHIREHMH* -2.553 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22266 SLTFKFNRLYFTLRAWGTFG 20 SLAY-screened peptide P616 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCTTACCTTCAAGTTCAATCGGCTGTATTTCACTCTGAGGGCCTGGGGCACCTTTGGCTAA SLTFKFNRLYFTLRAWGTFG* -2.553 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22267 PVQPNHCTRHASD 13 SLAY-screened peptide P617 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGTTCAGCCTAATCATTGCACTCGTCACGCTTCGGATTAGGCCACCCTTACCTATTACTAA PVQPNHCTRHASD*ATLTYY* -2.548 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22268 TRVPSFLRLFILRFRSRRILN 21 SLAY-screened peptide P618 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCGTGTTCCAAGTTTCCTTCGCTTGTTTATTCTACGATTCAGATCACGACGGATACTTAAC TRVPSFLRLFILRFRSRRILN -2.548 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22269 IFHLSNYSSIVPRWTRCYCV 20 SLAY-screened peptide P619 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTTCCATCTTTCTAATTATAGTTCTATTGTCCCCCGGTGGACCCGCTGCTATTGTGTTTAA IFHLSNYSSIVPRWTRCYCV* -2.547 1.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22270 DSRPRTQVRTNARGPPRCCR 20 SLAY-screened peptide P620 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATAGTAGGCCGAGGACCCAGGTCCGTACGAATGCTCGCGGGCCGCCTCGGTGTTGTCGTTAA DSRPRTQVRTNARGPPRCCR* -2.547 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22271 VCDPINTYPMPLFDMYFFFL 20 SLAY-screened peptide P621 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTGTGACCCCATTAATACCTATCCTATGCCGTTGTTTGACATGTATTTTTTCTTTCTGTAA VCDPINTYPMPLFDMYFFFL* -2.545 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22272 LHQRTRHHSP 10 SLAY-screened peptide P622 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCATCAGCGTACCCGGCATCACTCTCCTTAGTGCTAGCCTTTCCCTGTCCTCCACGTCTAA LHQRTRHHSP*C*PFPVLHV* -2.545 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22273 LFYTNYRAHEDYHNYFNTQQ 20 SLAY-screened peptide P623 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTTCTACACCAATTATCGCGCTCACGAGGACTATCACAATTATTTTAATACGCAGCAGTAA LFYTNYRAHEDYHNYFNTQQ* -2.542 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22274 NRRLALYPVCVCGVAS 16 SLAY-screened peptide P624 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCGTCGCCTGGCTTTGTATCCTGTTTGCGTGTGCGGTGTTGCCAGTTAGGTGAACCACTAA NRRLALYPVCVCGVAS*VNH* -2.541 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22275 RSFDDMLMPITLAFFSAVCP 20 SLAY-screened peptide P625 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAGCTTCGACGATATGCTCATGCCTATCACCCTGGCCTTTTTCTCCGCCGTGTGTCCGTAA RSFDDMLMPITLAFFSAVCP* -2.541 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22276 YKH 3 SLAY-screened peptide P626 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAAGCATTAGAGTAACTTTACTGACAACCCTCACCTTTCCAATTCTCAGCGCGGTCCCTAA YKH*SNFTDNPHLSNSQRGP* -2.537 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22277 LLRNNDLSRELINTNNQDLH 20 SLAY-screened peptide P627 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTTCGTAATAATGACCTGTCCCGGGAGCTTATTAATACCAACAATCAGGACCTGCATTAA LLRNNDLSRELINTNNQDLH* -2.537 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22278 PPTWFESALFFIFTILFRLVN 21 SLAY-screened peptide P628 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCACCTGGTTCGAAAGCGCCTTATTTTTCATATTTACTATACTGTTTAGATTAGTTAAC PPTWFESALFFIFTILFRLVN -2.535 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22279 EWVSNPTLRMLTSLDCPRTL 20 SLAY-screened peptide P629 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGTGGGTGTCTAACCCGACGCTGCGTATGCTCACTAGCTTGGATTGTCCTAGGACTCTTTAA EWVSNPTLRMLTSLDCPRTL* -2.534 0.000104 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22280 HNTSCRPPMDPITLDCRHKT 20 SLAY-screened peptide P630 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAACACTTCTTGTAGGCCTCCCATGGATCCCATCACGCTGGATTGCCGTCATAAGACGTAA HNTSCRPPMDPITLDCRHKT* -2.534 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22281 YLVHLRVAMYLKHHASHQVR 20 SLAY-screened peptide P631 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTTGGTCCATCTGCGCGTTGCGATGTATCTTAAGCACCACGCGTCTCACCAGGTGCGCTAA YLVHLRVAMYLKHHASHQVR* -2.531 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22282 PSRNTRSTMARTQTIRYTSR 20 SLAY-screened peptide P632 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGCAGGAATACGCGTAGCACCATGGCTCGCACTCAGACTATCCGTTATACTTCTCGGTAA PSRNTRSTMARTQTIRYTSR* -2.531 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22283 HRTPCRFFGVYVGVYISVTC 20 SLAY-screened peptide P633 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGGACCCCCTGTCGCTTCTTTGGTGTCTATGTTGGTGTCTATATTTCTGTTACGTGCTAA HRTPCRFFGVYVGVYISVTC* -2.529 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22284 RVTCMVSTNIHSAYNPAFII 20 SLAY-screened peptide P634 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGTCACGTGCATGGTCTCTACGAACATTCACTCGGCTTACAATCCGGCTTTTATCATCTAA RVTCMVSTNIHSAYNPAFII* -2.528 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22285 IARTYLNHSRSPPPAVP 17 SLAY-screened peptide P635 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGCTCGCACTTATCTCAATCATTCGCGGTCTCCGCCCCCGGCTGTGCCCTAACTGAGTAAG IARTYLNHSRSPPPAVP*LSK -2.527 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22286 WGAYRITSSRCIGKANMYID 20 SLAY-screened peptide P636 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGGGTGCTTATCGCATTACTAGCAGTCGGTGCATTGGTAAGGCGAATATGTACATTGATTAA WGAYRITSSRCIGKANMYID* -2.525 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22287 PNFRQSSIPENTLHCVVVLY 20 SLAY-screened peptide P637 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAATTTTCGCCAGTCTTCCATCCCGGAGAATACGCTGCATTGCGTTGTTGTTCTGTACTAA PNFRQSSIPENTLHCVVVLY* -2.524 3.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22288 DHDNFLEQVYYPRNRYASNS 20 SLAY-screened peptide P638 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCATGACAATTTCCTCGAGCAGGTTTATTACCCGCGTAATCGGTATGCTAGTAATTCTTAA DHDNFLEQVYYPRNRYASNS* -2.524 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22289 LARDGNYFGVRNTNLFSAHT 20 SLAY-screened peptide P639 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGCCCGTGACGGGAACTACTTTGGCGTGCGCAATACTAATCTTTTCAGCGCGCATACGTAA LARDGNYFGVRNTNLFSAHT* -2.524 0.000237 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22290 FGTSWSISYKRNFNVYRYKS 20 SLAY-screened peptide P640 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTGGCACCAGCTGGAGTATTAGTTATAAGCGCAACTTTAACGTTTACAGGTATAAGAGTTAA FGTSWSISYKRNFNVYRYKS* -2.52 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22291 LTYNTPIYYHVHIKSGRYDM 20 SLAY-screened peptide P641 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACCTATAATACGCCTATCTATTATCATGTGCACATTAAGTCTGGGCGGTACGATATGTAA LTYNTPIYYHVHIKSGRYDM* -2.519 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22292 EPCRSAAAWPNI 12 SLAY-screened peptide P642 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCCTTGTAGGTCTGCTGCTGCTTGGCCTAACATTTAGGATTGCTCGGGTCACACGACTTAA EPCRSAAAWPNI*DCSGHTT* -2.519 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22293 PRSAPTPAVYTSPALASAST 20 SLAY-screened peptide P643 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCGCTCCGCCCCCACGCCTGCCGTTTACACCTCGCCTGCGCTGGCGTCCGCTAGCACGTAA PRSAPTPAVYTSPALASAST* -2.519 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22294 NLCFSSLDSFITAAL 15 SLAY-screened peptide P644 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCTCTGCTTCTCTTCGCTTGACAGTTTCATTACGGCTGCGCTTTAGATCTATGATTACTAA NLCFSSLDSFITAAL*IYDY* -2.518 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22295 PNRVHDPCSMYTVYRKFHHS 20 SLAY-screened peptide P645 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAATCGTGTTCATGATCCGTGTAGCATGTACACGGTGTATCGCAAGTTCCATCACTCTTAA PNRVHDPCSMYTVYRKFHHS* -2.516 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22296 NIFSSCTILSRCGCNLIVEN 20 SLAY-screened peptide P646 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACATTTTCTCTTCTTGCACGATTCTTAGCCGTTGCGGTTGTAACCTCATTGTCGAGAATTAA NIFSSCTILSRCGCNLIVEN* -2.516 0.000344 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22297 CNFALPKLSTILHRLRSSLFN 21 SLAY-screened peptide P647 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAACTTCGCGCTCCCCAAGCTTTCCACTATCTTGCACCGATTGCGATCTTCACTCTTTAAC CNFALPKLSTILHRLRSSLFN -2.516 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22298 AHRAMGSLQGFFYTFYFLIP 20 SLAY-screened peptide P648 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCACCGTGCCATGGGTTCGCTCCAGGGTTTCTTTTACACTTTTTACTTTCTTATCCCCTAA AHRAMGSLQGFFYTFYFLIP* -2.515 0.000288 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22299 ISRRSTHNSDDYYRAPNISL 20 SLAY-screened peptide P649 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAGCCGTCGTAGCACTCATAATTCCGATGACTATTATAGGGCTCCTAACATCAGTCTGTAA ISRRSTHNSDDYYRAPNISL* -2.513 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22300 PLKVPNASNNLVRFTSPA 18 SLAY-screened peptide P650 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTAAGGTCCCTAATGCTTCGAATAACCTTGTTAGGTTTACTTCCCCGGCTTAGACGTAA PLKVPNASNNLVRFTSPA*T* -2.513 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22301 KPTGATHPLYSCRHTPHVNA 20 SLAY-screened peptide P651 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCCGACTGGTGCTACCCATCCTCTCTATTCTTGCAGGCATACTCCTCATGTGAATGCCTAA KPTGATHPLYSCRHTPHVNA* -2.513 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22302 YPSWQANAN 9 SLAY-screened peptide P652 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCCTAGCTGGCAGGCCAACGCGAATTAGAGTGATATTATTCTTGATTAGAATCATGATTAA YPSWQANAN*SDIILD*NHD* -2.512 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22303 RDRSLDLFCLSVHPQWDGHT 20 SLAY-screened peptide P653 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGACCGCAGTCTGGATTTGTTTTGTCTTTCGGTGCACCCCCAGTGGGACGGGCATACCTAA RDRSLDLFCLSVHPQWDGHT* -2.512 0.000172 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22304 SKYRVTPLAFFALYHHVTFS 20 SLAY-screened peptide P654 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCAAGTACCGCGTTACGCCTCTGGCTTTTTTCGCCCTTTACCACCACGTCACCTTCTCCTAA SKYRVTPLAFFALYHHVTFS* -2.512 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22305 ASISNSVAINYPHAHFPLLAN 21 SLAY-screened peptide P655 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAGTATCAGTAACTCGGTGGCTATTAATTATCCTCATGCTCACTTTCCCTTGCTGGCTAAC ASISNSVAINYPHAHFPLLAN -2.51 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22306 RLSLGLYNANSYTIWDVKYM 20 SLAY-screened peptide P656 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTCTCCCTCGGTCTTTATAATGCTAATAGTTACACCATTTGGGATGTCAAGTATATGTAA RLSLGLYNANSYTIWDVKYM* -2.508 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22307 YKTARLTDATAFLSPCSYHT 20 SLAY-screened peptide P657 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAAGACGGCCCGTCTCACTGATGCTACTGCGTTTTTGTCTCCTTGTTCTTATCATACCTAA YKTARLTDATAFLSPCSYHT* -2.506 4.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22308 RQHPQHACDPD 11 SLAY-screened peptide P658 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCAGCATCCCCAGCATGCCTGTGACCCCGATTAGAATCCTAGTCTGTATTGTAACGTTTAA RQHPQHACDPD*NPSLYCNV* -2.505 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22309 TLIFFACQIFVLPGSAHFRVS 21 SLAY-screened peptide P659 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTGATTTTTTTTGCTTGTCAGATTTTTGTCTTGCCCGGTAGTGCTCACTTTAGAGTAAGT TLIFFACQIFVLPGSAHFRVS -2.504 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22310 YSPNSQEGTCATNTHHILIL 20 SLAY-screened peptide P660 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTCCCCTAACTCTCAGGAGGGTACGTGTGCTACTAACACGCATCATATCCTGATCCTTTAA YSPNSQEGTCATNTHHILIL* -2.502 0.000177 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22311 LLHTLSEHPFFDINVCDSAS 20 SLAY-screened peptide P661 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCTCCATACCCTCTCGGAGCATCCCTTCTTTGACATTAACGTTTGTGATAGTGCTTCTTAA LLHTLSEHPFFDINVCDSAS* -2.501 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22312 LPTTVYLSVCPSTGGILVPH 20 SLAY-screened peptide P662 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCTACGACTGTGTACTTGTCTGTTTGCCCTTCTACTGGCGGCATTCTTGTCCCTCACTAA LPTTVYLSVCPSTGGILVPH* -2.501 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22313 MIHFWVLPGRLLFIG 15 SLAY-screened peptide P663 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGATTCACTTTTGGGTCCTACCGGGCCGTTTGCTCTTCATAGGTTGAATAAGCACCTGTAAC MIHFWVLPGRLLFIG*ISTCN -2.5 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22314 HLTSYDR 7 SLAY-screened peptide P664 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCTCACGTCGTACGATCGCTAGACTGATTGTGACAGCTTTAACGACAATTTTGACTCCTAA HLTSYDR*TDCDSFNDNFDS* -2.5 0.001751 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22315 SDRRVMLSFSFSDRPGVDLQA 21 SLAY-screened peptide P665 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGACCGCCGCGTTATGCTCTCTTTCTCGTTTTCTGACCGCCCTGGTGTCGACCTGCAGGCA SDRRVMLSFSFSDRPGVDLQA -2.499 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22316 PCHHKIRRKCTLVHRPPNAL 20 SLAY-screened peptide P666 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCCATCACAAGATTCGCCGCAAGTGTACGCTTGTCCATAGGCCGCCCAACGCTCTCTAA PCHHKIRRKCTLVHRPPNAL* -2.498 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22317 HRSLHMYRNFCFNFDCE 17 SLAY-screened peptide P667 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGTAGCCTGCACATGTATCGTAATTTTTGCTTTAATTTTGACTGTGAGTAGTACCAGTAA HRSLHMYRNFCFNFDCE*YQ* -2.497 0.000281 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22318 ILFPARILRLLKNFYYLKHN 20 SLAY-screened peptide P668 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCTTTTTCCGGCGCGCATCTTGCGCTTGCTTAAGAATTTTTATTACCTCAAGCATAACTAA ILFPARILRLLKNFYYLKHN* -2.497 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22319 TATAHKKRNNPLLTVAMGVV 20 SLAY-screened peptide P669 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGCTACGGCTCATAAGAAGCGGAACAATCCCCTTTTGACGGTGGCTATGGGCGTGGTCTAA TATAHKKRNNPLLTVAMGVV* -2.495 0.000297 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22320 LLLHRLLYGNHCMLTHDTSC 20 SLAY-screened peptide P670 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTTGCTGCATCGCCTCTTGTATGGGAATCATTGCATGCTTACGCACGACACTAGTTGCTAA LLLHRLLYGNHCMLTHDTSC* -2.495 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22321 AVPACPCLTVPDRDVPSNTV 20 SLAY-screened peptide P671 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGTGCCGGCTTGCCCTTGCCTTACTGTTCCGGACCGCGATGTTCCGAGTAATACTGTGTAA AVPACPCLTVPDRDVPSNTV* -2.494 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22322 PHASRLHGAYDQRFSCYNPSN 21 SLAY-screened peptide P672 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCACGCCTCGCGCCTGCATGGCGCTTATGATCAGCGCTTCTCTTGTTATAATCCTTCTAAC PHASRLHGAYDQRFSCYNPSN -2.493 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22323 PLAQNTDILCINYFVISTPM 20 SLAY-screened peptide P673 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTTGCCCAGAACACTGATATCCTTTGCATCAATTACTTCGTCATCAGTACGCCTATGTAA PLAQNTDILCINYFVISTPM* -2.489 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22324 IGERPAKTLTAGHDGGYTLAN 21 SLAY-screened peptide P674 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGGCGAGCGGCCGGCGAAGACGCTTACCGCGGGCCATGACGGGGGCTATACGCTTGCTAAC IGERPAKTLTAGHDGGYTLAN -2.489 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22325 SVVSAPRDRYRAPSNPRSYG 20 SLAY-screened peptide P675 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGTCGTCTCTGCGCCTCGCGATCGTTATCGGGCTCCGTCTAATCCCAGGTCCTACGGTTAA SVVSAPRDRYRAPSNPRSYG* -2.488 0.000102 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22326 ECSATYAVPGDQYPNYFILL 20 SLAY-screened peptide P676 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGTGCTCGGCTACTTATGCCGTCCCTGGTGACCAGTACCCTAACTACTTTATTCTCCTTTAA ECSATYAVPGDQYPNYFILL* -2.487 2.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22327 FPYRICYNRLSFNSHLHDAT 20 SLAY-screened peptide P677 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCCTTATCGGATCTGCTACAATCGCCTTTCTTTTAACTCGCATCTTCACGATGCGACCTAA FPYRICYNRLSFNSHLHDAT* -2.487 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22328 PLNPDSNPEHASLCHSEVFY 20 SLAY-screened peptide P678 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCAACCCTGACTCTAACCCTGAGCATGCTTCGCTTTGCCACAGTGAGGTCTTCTACTAA PLNPDSNPEHASLCHSEVFY* -2.486 2.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22329 SRIECTPLSNVGLDPGCALN 20 SLAY-screened peptide P679 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCGTATCGAGTGCACCCCTCTGTCTAATGTTGGGCTTGATCCTGGTTGCGCTTTGAATTAA SRIECTPLSNVGLDPGCALN* -2.486 0.001213 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22330 CVLEINVNHYWHHREALFNI 20 SLAY-screened peptide P680 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGTTCTCGAAATTAATGTCAATCACTACTGGCATCACCGTGAGGCGCTCTTCAATATTTAA CVLEINVNHYWHHREALFNI* -2.485 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22331 IPRSMCPADSNVQDKGHSGP 20 SLAY-screened peptide P681 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCCGCGTAGCATGTGCCCTGCTGACTCCAATGTCCAGGACAAGGGTCACAGCGGCCCCTAA IPRSMCPADSNVQDKGHSGP* -2.485 0.000198 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22332 ANTMAKIHCNKVLGAIPHVL 20 SLAY-screened peptide P682 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAATACCATGGCCAAGATTCATTGTAATAAGGTGCTTGGGGCTATTCCGCATGTTCTGTAA ANTMAKIHCNKVLGAIPHVL* -2.483 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22333 HPFHIYCRSDSSNRRLACGN 20 SLAY-screened peptide P683 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCCGTTTCACATTTATTGTCGCAGTGACAGCAGCAATCGCCGACTCGCTTGTGGTAACTGA HPFHIYCRSDSSNRRLACGN* -2.479 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22334 PYQSKLYHNLHRSNLCVHGD 20 SLAY-screened peptide P684 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTATCAGTCCAAGCTGTACCATAACCTTCATCGTTCGAATCTGTGTGTGCATGGGGACTAA PYQSKLYHNLHRSNLCVHGD* -2.479 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22335 SSPARGDLDFCRTFNNIQIT 20 SLAY-screened peptide P685 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTCCCCCGCCCGTGGCGATCTGGACTTTTGCCGTACGTTTAATAATATCCAGATCACCTAA SSPARGDLDFCRTFNNIQIT* -2.478 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22336 GSACTSQFPHFTLINGHGTN 20 SLAY-screened peptide P686 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTCTGCTTGCACGAGCCAGTTCCCGCACTTTACTCTCATCAACGGCCATGGTACTAATTAA GSACTSQFPHFTLINGHGTN* -2.478 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22337 TMHYYKSHTLYHSNTGPTHY 20 SLAY-screened peptide P687 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCATGCATTATTATAAGAGCCACACTTTGTACCACAGCAATACCGGCCCCACTCACTACTAA TMHYYKSHTLYHSNTGPTHY* -2.477 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22338 DRNIPIRFVCGHNHGPLIFN 20 SLAY-screened peptide P688 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCGTAATATCCCCATCCGGTTCGTTTGTGGTCACAACCACGGGCCTCTTATTTTTAATTAA DRNIPIRFVCGHNHGPLIFN* -2.477 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22339 LRWAPSYSRRDFRLKFGDIR 20 SLAY-screened peptide P689 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCGGTGGGCCCCTAGTTATTCTCGTAGGGATTTCCGCCTGAAGTTCGGGGACATTCGTTAA LRWAPSYSRRDFRLKFGDIR* -2.476 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22340 LPRYVNTIPDISCTIPRRSVN 21 SLAY-screened peptide P690 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCGCGTTACGTTAACACTATCCCTGACATATCCTGTACCATTCCACGACGGTCAGTTAAC LPRYVNTIPDISCTIPRRSVN -2.476 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22341 HETPYHHRALAPVPASLLFPE 21 SLAY-screened peptide P691 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGAGACGCCTTATCACCACAGGGCCCTAGCACCAGTGCCTGCGTCACTACTTTTCCCCGAG HETPYHHRALAPVPASLLFPE -2.472 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22342 HRNTLRLHVGLKACVTLFNN 20 SLAY-screened peptide P692 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAGGAACACGCTCCGTCTGCACGTGGGGCTTAAGGCGTGTGTTACGCTTTTTAATAATTAA HRNTLRLHVGLKACVTLFNN* -2.472 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22343 TRSYKSRHMGGYISISIITFN 21 SLAY-screened peptide P693 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCGCAGTTATAAGTCTCGGCACATGGGCGGTTATATTTCTATTTCGATCATAACATTTAAC TRSYKSRHMGGYISISIITFN -2.469 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22344 FTHPSYRRSHCVRLASLGMN 20 SLAY-screened peptide P694 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCACTCACCCGTCTTATCGCCGCAGTCATTGTGTTCGGTTGGCTAGTTTGGGTATGAACTAA FTHPSYRRSHCVRLASLGMN* -2.469 0.000144 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22345 TLHGIFTFFVAGSLGVLS 18 SLAY-screened peptide P695 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTTGCACGGTATTTTTACGTTCTTTGTGGCCGGCTCTCTGGGAGTTTTGAGTTGAACTAAC TLHGIFTFFVAGSLGVLS*TN -2.469 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22346 LRLTAHIHLGTYPIVDVTSY 20 SLAY-screened peptide P696 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCGGCTCACCGCCCACATTCATCTTGGTACCTACCCCATTGTTGACGTTACCAGCTACTAA LRLTAHIHLGTYPIVDVTSY* -2.467 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22347 PVFALRSAVKSAAST 15 SLAY-screened peptide P697 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGTGTTTGCCCTGCGTAGCGCTGTTAAAAGCGCTGCGAGCACTTGAACTTTAGGACGTAAC PVFALRSAVKSAAST*TLGRN -2.466 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22348 HRIEFFGHSCTNLCDYYHGS 20 SLAY-screened peptide P698 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCGCATCGAGTTTTTCGGTCATTCTTGTACTAACCTGTGCGACTATTATCACGGGAGCTAA HRIEFFGHSCTNLCDYYHGS* -2.466 0.000107 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22349 FVDHIACPHSSPFYSIIFRI 20 SLAY-screened peptide P699 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTGTTGACCATATTGCTTGCCCCCACTCGTCCCCTTTTTATAGTATTATTTTTAGGATCTAA FVDHIACPHSSPFYSIIFRI* -2.465 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22350 PGVSTVFCVNAHSSYFRFCR 20 SLAY-screened peptide P700 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGTGTCTCGACTGTCTTCTGTGTGAACGCTCATAGTTCCTATTTTCGTTTTTGTCGGTAA PGVSTVFCVNAHSSYFRFCR* -2.464 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22351 PWSVGALRAYWGHGGPRPDE 20 SLAY-screened peptide P701 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGGTCTGTCGGCGCTTTGCGTGCTTATTGGGGCCATGGTGGGCCGCGTCCTGACGAGTAA PWSVGALRAYWGHGGPRPDE* -2.463 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22352 LHFILDASRVCHHRKGN 17 SLAY-screened peptide P702 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCATTTCATTCTTGACGCTTCTCGGGTCTGTCACCATAGGAAGGGCAATTAGCAGACTTAA LHFILDASRVCHHRKGN*QT* -2.462 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22353 SSTNHHKCTRLKSNNVIMAG 20 SLAY-screened peptide P703 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCAGTACTAATCACCATAAGTGTACTCGCCTTAAGAGCAATAATGTTATCATGGCGGGTTAA SSTNHHKCTRLKSNNVIMAG* -2.46 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22354 RYLGQSNNSCCAASGLPINT 20 SLAY-screened peptide P704 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTATTTGGGTCAGTCCAATAATTCGTGCTGTGCTGCGTCGGGTCTCCCTATCAACACTTAA RYLGQSNNSCCAASGLPINT* -2.459 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22355 WRIVLCPKLHDLLYNNMHCN 20 SLAY-screened peptide P705 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCGTATTGTCCTGTGTCCCAAGCTGCACGACCTCCTTTACAATAATATGCATTGCAATTAA WRIVLCPKLHDLLYNNMHCN* -2.458 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22356 SCAAPSCTSYPRDKITPYSW 20 SLAY-screened peptide P706 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTGTGCCGCTCCGTCTTGTACCTCTTATCCGAGGGACAAGATCACTCCTTATTCGTGGTAA SCAAPSCTSYPRDKITPYSW* -2.456 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22357 VYSLGSNPDNYN 12 SLAY-screened peptide P707 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTACTCCTTGGGGTCGAACCCTGACAATTATAATTAGACTTGTATCCGCATTCTTACTTAA VYSLGSNPDNYN*TCIRILT* -2.456 2.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22358 YTPALCPGLSSNRVNRSSAQ 20 SLAY-screened peptide P708 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACTCCTGCGCTGTGTCCTGGGCTCTCTAGTAATAGGGTTAATCGCTCTTCTGCCCAGTAA YTPALCPGLSSNRVNRSSAQ* -2.456 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22359 GAEGLSVLTVNIFTKYCRHG 20 SLAY-screened peptide P709 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGCCGAGGGTTTGTCCGTTCTTACTGTTAATATCTTTACCAAGTATTGTAGGCATGGGTAA GAEGLSVLTVNIFTKYCRHG* -2.455 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22360 PSNPNHLVNSSDVVHCYYPR 20 SLAY-screened peptide P710 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTCCAACCCCAACCATCTTGTCAATTCGTCGGATGTTGTGCACTGCTACTATCCGCGCTAA PSNPNHLVNSSDVVHCYYPR* -2.455 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22361 AGWTLHTVMRAHTPTDCAYN 20 SLAY-screened peptide P711 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGGTTGGACTCTTCATACTGTTATGCGTGCCCATACCCCGACTGATTGCGCTTATAACTAA AGWTLHTVMRAHTPTDCAYN* -2.451 3.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22362 AACCRSQNVSLNLLFTFNRY 20 SLAY-screened peptide P712 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGCGTGTTGTAGGTCTCAGAATGTCTCTCTTAATCTTCTCTTTACGTTTAACCGGTACTAA AACCRSQNVSLNLLFTFNRY* -2.45 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22363 PLTDWWTSL 9 SLAY-screened peptide P713 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTGACTGATTGGTGGACTTCTCTCTAGTCGGCTACGGGCAATTATGTGCACGCGCTTTAA PLTDWWTSL*SATGNYVHAL* -2.45 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22364 GFGAAPWDPVSSY 13 SLAY-screened peptide P714 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTTCGGCGCTGCGCCGTGGGATCCCGTTAGTTCGTATTAGAACCATAATACTGCTGATTAA GFGAAPWDPVSSY*NHNTAD* -2.448 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22365 ILWLRFRGTIIIWKFRFRLVN 21 SLAY-screened peptide P715 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCTGTGGCTGCGTTTTCGGGGGACTATTATCATTTGGAAATTTAGGTTCCGTCTAGTTAAC ILWLRFRGTIIIWKFRFRLVN -2.442 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22366 SHAIHMAHSHFYCVSHENTS 20 SLAY-screened peptide P716 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCATGCCATCCATATGGCTCATTCGCATTTTTACTGTGTTAGCCATGAGAATACTAGTTAA SHAIHMAHSHFYCVSHENTS* -2.442 6.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22367 GPWSRHIYYSLIFYSIIVAA 20 SLAY-screened peptide P717 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCCTTGGAGTAGGCACATCTATTATAGCCTCATTTTCTATAGCATTATTGTTGCCGCCTAA GPWSRHIYYSLIFYSIIVAA* -2.441 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22368 QNQAC 5 SLAY-screened peptide P718 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGAACCAGGCTTGCTAGCATTTTGTCAACCCTATTGCTGTCCGTCTGATTCATAACTCCTAA QNQAC*HFVNPIAVRLIHNS* -2.44 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22369 MYLS 4 SLAY-screened peptide P719 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTACCTGTCCTAGTCTCTCCACAAGGTTGATATTAGTTCTCCTATTTCCCTGCTTTCGTAA MYLS*SLHKVDISSPISLLS* -2.439 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22370 VSMQRPNGKFNITV 14 SLAY-screened peptide P720 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTCTATGCAGAGGCCTAACGGTAAGTTCAACATCACTGTTTAGGATCCGGTTTAGTGTTAA VSMQRPNGKFNITV*DPV*C* -2.439 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22371 RAHCMPYYLFSTLTPEVILL 20 SLAY-screened peptide P721 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCTCATTGCATGCCTTATTATTTGTTTAGTACTCTCACCCCTGAGGTCATTCTGCTTTAA RAHCMPYYLFSTLTPEVILL* -2.439 0.000386 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22372 GYHTHSRAPHELDYRIISAI 20 SLAY-screened peptide P722 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTACCATACTCATTCGCGGGCGCCCCATGAGCTTGACTATCGGATTATCAGCGCGATTTAA GYHTHSRAPHELDYRIISAI* -2.438 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22373 LFFAPK 6 SLAY-screened peptide P723 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTTTTTCGCGCCGAAGTAGAACCTGAACGCTTTGCACGGTAAGTATCCTCTCATGGATTAA LFFAPK*NLNALHGKYPLMD* -2.437 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22374 SF 2 SLAY-screened peptide P724 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTTCTAGGTGCGGCCCTTGGTTATCCCGCTTTTTGCTCCGTCGCCCATGGCGTACTCTTAA SF*VRPLVIPLFAPSPMAYS* -2.436 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22375 LSGLCFAIIGCWNTY 15 SLAY-screened peptide P725 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTCTGGGCTTTGTTTTGCGATTATCGGTTGTTGGAATACTTACTAGCTTTCCTGTGTTTAA LSGLCFAIIGCWNTY*LSCV* -2.435 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22376 TVSSTSSGSYVDGSNFVYLF 20 SLAY-screened peptide P726 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTTTCTTCTACCTCGAGTGGCTCGTATGTCGATGGCAGTAATTTTGTCTATCTGTTTTAA TVSSTSSGSYVDGSNFVYLF* -2.435 0.000669 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22377 TPYHCVHRPTRWQRCCRDPP 20 SLAY-screened peptide P727 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCGTATCACTGTGTTCACCGGCCCACGAGGTGGCAGCGTTGCTGTCGTGACCCTCCCTAA TPYHCVHRPTRWQRCCRDPP* -2.435 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22378 PQHPCPLPPIYYMPAMLTPL 20 SLAY-screened peptide P728 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCAGCACCCTTGTCCGTTGCCGCCCATTTATTACATGCCTGCGATGCTTACTCCGCTGTAA PQHPCPLPPIYYMPAMLTPL* -2.433 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22379 VAEHPHLRHDGHCGVFYLTA 20 SLAY-screened peptide P729 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGCCGAGCATCCCCACCTGCGCCACGATGGTCACTGCGGCGTCTTTTATCTCACCGCCTAA VAEHPHLRHDGHCGVFYLTA* -2.431 0.005318 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22380 IYPPIGYPVYL 11 SLAY-screened peptide P730 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTATCCTCCGATTGGTTATCCGGTGTATCTGTAGAATACTGAGTCCATCGATTATATGTAA IYPPIGYPVYL*NTESIDYM* -2.43 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22381 HATGTRIHAFSPIPVRTHIP 20 SLAY-screened peptide P731 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGCGACTGGCACTAGGATTCACGCTTTCTCGCCTATTCCGGTCCGTACGCATATTCCCTAA HATGTRIHAFSPIPVRTHIP* -2.426 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22382 PSIQICYPLLCNTYLSNTRN 20 SLAY-screened peptide P732 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAGCATCCAGATTTGTTACCCCTTGCTTTGCAATACTTATCTGTCCAACACTAGGAATTAA PSIQICYPLLCNTYLSNTRN* -2.426 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22383 RTNFDLTHWSQGHWIAYPLA 20 SLAY-screened peptide P733 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGACGAACTTTGATCTTACGCACTGGTCCCAGGGTCACTGGATTGCTTATCCTCTTGCCTAA RTNFDLTHWSQGHWIAYPLA* -2.426 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22384 GRDHILDLDFNGGSSARNDI 20 SLAY-screened peptide P734 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCAGGGACCATATTTTGGACCTGGATTTTAACGGTGGTTCTTCTGCGCGTAATGATATTTAA GRDHILDLDFNGGSSARNDI* -2.425 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22385 SVRNGTIAPCVSRPSAWRPTN 21 SLAY-screened peptide P735 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGTTCGTAATGGTACGATAGCCCCGTGCGTATCAAGGCCGTCTGCTTGGCGGCCCACTAAC SVRNGTIAPCVSRPSAWRPTN -2.423 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22386 FPTGVYSPVYCPISNCTFDY 20 SLAY-screened peptide P736 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCCGACCGGCGTTTATTCCCCTGTTTACTGCCCCATTAGTAATTGTACTTTTGATTATTAA FPTGVYSPVYCPISNCTFDY* -2.419 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22387 LLLMFFSLLLLLVAVADVLTE 21 SLAY-screened peptide P737 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTGCTCATGTTCTTTTCTCTCTTGTTGTTATTGGTCGCCGTCGCAGACGTTCTAACTGAG LLLMFFSLLLLLVAVADVLTE -2.416 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22388 GSRRCLPCHDTLPNPTEPSS 20 SLAY-screened peptide P738 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGAGTAGGCGTTGTCTTCCCTGTCATGATACGTTGCCGAATCCCACTGAGCCGAGTTCTTAA GSRRCLPCHDTLPNPTEPSS* -2.415 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22389 LSCDPVYALAIKHPVILDYT 20 SLAY-screened peptide P739 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAGTTGTGACCCTGTGTATGCCCTCGCTATTAAGCATCCTGTTATTCTCGACTATACGTAA LSCDPVYALAIKHPVILDYT* -2.412 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22390 SYNMTSICIFVERCPSAIRVN 21 SLAY-screened peptide P740 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTACAATATGACTTCTATCTGCATCTTTGTGGAAAGATGCCCGAGTGCTATTCGCGTTAAC SYNMTSICIFVERCPSAIRVN -2.408 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22391 SSQYDSCRNLFNWLSMPVNI 20 SLAY-screened peptide P741 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCAGCCAGTATGATTCGTGCCGTAACTTGTTTAACTGGCTTTCTATGCCTGTGAATATTTAA SSQYDSCRNLFNWLSMPVNI* -2.408 3.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22392 ASSHYNRGSYLAFPS 15 SLAY-screened peptide P742 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTAGTAGTCACTACAATCGCGGTAGTTATCTCGCTTTTCCTTCCTAGACGGTCAGGAATTAA ASSHYNRGSYLAFPS*TVRN* -2.408 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22393 PAEGHHLHGNTSVSKMPRTL 20 SLAY-screened peptide P743 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGCCGAGGGCCACCACCTGCATGGGAACACCAGCGTTTCCAAGATGCCGCGCACTCTGTAA PAEGHHLHGNTSVSKMPRTL* -2.406 0.000527 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22394 GPLLAGYTFGNTSGSFRYGL 20 SLAY-screened peptide P744 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCCCTTGCTCGCGGGGTACACTTTCGGTAACACTTCTGGCTCGTTTCGTTACGGGTTGTAA GPLLAGYTFGNTSGSFRYGL* -2.405 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22395 KQGYCGWPAVTYFPLSFSFH 20 SLAY-screened peptide P745 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCAGGGTTATTGTGGTTGGCCTGCGGTCACTTACTTTCCTCTCAGCTTCTCTTTCCATTAA KQGYCGWPAVTYFPLSFSFH* -2.405 0.00035 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22396 SYRATAIQVAGINVNNA 17 SLAY-screened peptide P746 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTACCGGGCCACTGCTATCCAGGTTGCTGGCATTAATGTTAATAATGCTTAGTAGACCTAA SYRATAIQVAGINVNNA**T* -2.405 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22397 SCTGWSLPSYGHPAFISPNS 20 SLAY-screened peptide P747 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTGTACTGGCTGGTCGCTCCCTTCTTACGGCCATCCTGCTTTTATTAGTCCTAATTCTTAA SCTGWSLPSYGHPAFISPNS* -2.405 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22398 IEHPFCLSMPAYNVNKVYEC 20 SLAY-screened peptide P748 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGAGCATCCCTTCTGTCTGTCGATGCCGGCTTACAATGTTAATAAGGTCTATGAGTGTTAA IEHPFCLSMPAYNVNKVYEC* -2.404 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22399 ADYCRPFT 8 SLAY-screened peptide P749 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGACTACTGTAGGCCCTTTACCTAGAAGATTAAGACTCATCATTTGTAGATTAGGGTTTAA ADYCRPFT*KIKTHHL*IRV* -2.403 3.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22400 KLYHNTYIIHCRSTLVPCNL 20 SLAY-screened peptide P750 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCTTTATCATAATACCTACATTATCCATTGCCGCTCTACGCTTGTCCCTTGCAATCTGTAA KLYHNTYIIHCRSTLVPCNL* -2.402 6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22401 PFLHFSFPRRFITVEHRSSC 20 SLAY-screened peptide P751 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTTCTCCATTTCTCTTTCCCTCGCCGTTTTATTACCGTGGAGCATAGGTCCAGCTGCTAA PFLHFSFPRRFITVEHRSSC* -2.402 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22402 HGILHPPLYSVNNSQVGWMCN 21 SLAY-screened peptide P752 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGGTATCTTGCACCCTCCCCTCTATTCTGTCAATAACAGTCAGGTTGGGTGGATGTGTAAC HGILHPPLYSVNNSQVGWMCN -2.4 1.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22403 HYHYRTAHDSKSNLLDLLTG 20 SLAY-screened peptide P753 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTACCACTACCGCACTGCTCATGATTCTAAGTCTAACTTGCTCGATTTGCTCACTGGTTAA HYHYRTAHDSKSNLLDLLTG* -2.4 0.000211 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22404 THPNLPLRAPLLPEAAQTNA 20 SLAY-screened peptide P754 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCATCCGAACCTCCCCTTGCGTGCCCCCCTTCTTCCGGAGGCGGCGCAGACTAATGCTTAA THPNLPLRAPLLPEAAQTNA* -2.399 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22405 LSLYLIVSPSETC 13 SLAY-screened peptide P755 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCGCTGTATCTTATCGTTTCCCCTTCGGAGACGTGCTAGAATGATCTTCGCAATCTGTAA LSLYLIVSPSETC*NDLRNL* -2.398 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22406 NRLDLRSIYKPHNFDIYFIY 20 SLAY-screened peptide P756 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCGCTTGGACCTCCGGTCTATCTATAAGCCCCATAACTTCGATATTTACTTTATTTATTAA NRLDLRSIYKPHNFDIYFIY* -2.398 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22407 TGTLPCNCQAIAGINLVVSI 20 SLAY-screened peptide P757 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGTACTCTCCCTTGCAATTGCCAGGCTATTGCTGGTATTAATCTCGTGGTTTCCATCTAA TGTLPCNCQAIAGINLVVSI* -2.397 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22408 RRHRLFSLYHTAPSLCSYDP 20 SLAY-screened peptide P758 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCGTCACCGTCTCTTTAGCCTTTACCACACCGCTCCTTCGCTGTGCTCTTATGATCCGTAA RRHRLFSLYHTAPSLCSYDP* -2.396 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22409 SQGTTYNLLMLSHVNSHS 18 SLAY-screened peptide P759 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCAGGGGACCACCTATAACCTCTTGATGTTGTCGCATGTGAATTCTCACAGCTAGTGCTAA SQGTTYNLLMLSHVNSHS*C* -2.392 1.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22410 TCPIILVYNLSTCNMTGSHR 20 SLAY-screened peptide P760 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGTCCTATTATTCTCGTTTACAACTTGTCGACGTGCAACATGACTGGGTCTCATCGGTAA TCPIILVYNLSTCNMTGSHR* -2.389 2.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22411 LEDSGAVFSGSCHIPPMSLV 20 SLAY-screened peptide P761 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGAGGATAGTGGGGCGGTGTTCAGTGGCTCCTGCCATATTCCTCCGATGTCTCTTGTGTAA LEDSGAVFSGSCHIPPMSLV* -2.388 0.001076 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22412 QFYT 4 SLAY-screened peptide P762 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTTTTATACCTAGCTTGCTAATGGGTAGCTTAAGGCTAACATTGAGACCATTCACGAGTAA QFYT*LANG*LKANIETIHE* -2.386 7.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22413 GCTPVTFHNVPYLITNNGFH 20 SLAY-screened peptide P763 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTGCACTCCTGTCACGTTTCATAATGTTCCTTATCTCATCACCAATAATGGGTTTCATTAA GCTPVTFHNVPYLITNNGFH* -2.386 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22414 CLQPYDIS 8 SLAY-screened peptide P764 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCTGCAGCCTTACGACATTTCTTAGTATATTGCTGACGTGTCCATGAGGATGGTTGTTTAA CLQPYDIS*YIADVSMRMVV* -2.383 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22415 PHLHPRPPRRSVPHCLLMWF 20 SLAY-screened peptide P765 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCACCTGCATCCGCGGCCCCCCCGCCGGTCTGTGCCCCACTGTCTGCTGATGTGGTTCTAA PHLHPRPPRRSVPHCLLMWF* -2.382 0.000489 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22416 PCPALLIISPLSLQPSLLIF 20 SLAY-screened peptide P766 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGCCCCGCCCTGCTCATCATCAGTCCTTTGTCCCTGCAGCCTTCCCTTCTGATCTTTTAA PCPALLIISPLSLQPSLLIF* -2.382 0.000154 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22417 RMLSCTPRLPSRRPYPGPGPN 21 SLAY-screened peptide P767 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCATGCTTTCGTGTACCCCCAGGCTGCCCTCTCGTCGCCCTTACCCTGGGCCCGGTCCTAAC RMLSCTPRLPSRRPYPGPGPN -2.381 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22418 GIDFYNHVYIFVEPSPSPLCN 21 SLAY-screened peptide P768 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCATCGACTTTTATAATCACGTGTACATTTTCGTGGAGCCTAGCCCTTCTCCGCTATGTAAC GIDFYNHVYIFVEPSPSPLCN -2.38 2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22419 CYNSFLRKRRKFKVYLTCLS 20 SLAY-screened peptide P769 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTACAATTCCTTCCTTCGTAAGCGTCGCAAGTTTAAGGTTTACCTCACCTGTCTCTCCTAA CYNSFLRKRRKFKVYLTCLS* -2.38 9.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22420 TSPHRHLNYPPLPVLNT 17 SLAY-screened peptide P770 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTCTCCTCACAGGCACCTTAACTACCCTCCGTTGCCGGTGCTTAACACTTAGACTCGGTAA TSPHRHLNYPPLPVLNT*TR* -2.378 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22421 ASDNHRRLGVTITFSVYFNF 20 SLAY-screened peptide P771 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCTCTGACAACCATCGCCGTCTGGGTGTTACGATCACGTTTTCTGTTTACTTTAACTTTTAA ASDNHRRLGVTITFSVYFNF* -2.378 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22422 TSYGSIPYVLALGSRNPNA 19 SLAY-screened peptide P772 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAGTTACGGTAGCATTCCCTACGTTCTGGCTCTTGGCTCTCGCAATCCCAACGCCTAACTG TSYGSIPYVLALGSRNPNA*L -2.378 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22423 AMAHCGKPIVPHHRLCY 17 SLAY-screened peptide P773 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCATGGCTCACTGCGGCAAGCCTATTGTTCCGCATCACCGTCTCTGTTACTAGGACACTTAA AMAHCGKPIVPHHRLCY*DT* -2.378 0.000287 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22424 SGCRRESFSRLRDRFYSNRV 20 SLAY-screened peptide P774 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGGGTGCCGCAGGGAGTCGTTCAGTAGGCTGCGTGATCGCTTTTACAGTAACCGTGTTTAA SGCRRESFSRLRDRFYSNRV* -2.378 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22425 GNDRRYTNVTTDIRSNFIML 20 SLAY-screened peptide P775 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTAATGACCGTCGCTACACCAACGTCACCACGGATATTAGGTCTAATTTTATTATGTTGTAA GNDRRYTNVTTDIRSNFIML* -2.378 0.00195 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22426 LAAPK 5 SLAY-screened peptide P776 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGGCTGCTCCGAAGTAGGCGCATGGGTACTCTGACTATTGCCCCACCCAGATCGTCTCGTAA LAAPK*AHGYSDYCPTQIVS* -2.377 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22427 APWRPRGRAWRSIFTYRRKA 20 SLAY-screened peptide P777 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCCTTGGCGGCCCCGGGGCCGCGCGTGGCGGAGTATCTTTACGTATCGTCGCAAGGCCTAA APWRPRGRAWRSIFTYRRKA* -2.376 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22428 KSSFLDGRWYYSNLHNRGLA 20 SLAY-screened peptide P778 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGAGCTCCTTTCTCGACGGTCGGTGGTATTATAGCAACCTGCATAATCGTGGTCTTGCGTAA KSSFLDGRWYYSNLHNRGLA* -2.376 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22429 LSPMSLLHTGVMRCNVNADF 20 SLAY-screened peptide P779 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCCCCTATGTCCCTCCTGCACACTGGCGTTATGCGTTGTAACGTTAACGCTGACTTCTAA LSPMSLLHTGVMRCNVNADF* -2.375 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22430 AFTPSVKNPSPANGAPNDCI 20 SLAY-screened peptide P780 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTTTTACCCCTTCCGTTAAGAACCCTTCTCCCGCCAATGGTGCCCCCAATGACTGCATTTAA AFTPSVKNPSPANGAPNDCI* -2.375 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22431 IPEILLSLYRRYLNVYKWVH 20 SLAY-screened peptide P781 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCCGGAGATCCTCCTCTCTCTTTACCGCCGTTACTTGAACGTTTACAAGTGGGTCCATTAA IPEILLSLYRRYLNVYKWVH* -2.374 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22432 PVVHLNLLRYLWSIYSNAVL 20 SLAY-screened peptide P782 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGTGGTGCACCTCAACTTGCTGAGGTATCTGTGGAGCATTTACAGTAATGCGGTTCTTTAA PVVHLNLLRYLWSIYSNAVL* -2.373 0.000335 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22433 SLGIYDGRIGGTNLNREKAY 20 SLAY-screened peptide P783 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTTGGGCATCTATGATGGTCGCATTGGCGGGACTAATCTTAATCGGGAGAAGGCTTACTAA SLGIYDGRIGGTNLNREKAY* -2.373 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22434 VTIVQPIPLSPPDSATCMHQ 20 SLAY-screened peptide P784 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTACTATTGTGCAGCCGATTCCCCTGTCCCCTCCTGACTCGGCCACTTGCATGCATCAGTAA VTIVQPIPLSPPDSATCMHQ* -2.372 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22435 GSTWYYNSTIRANYIIDNTR 20 SLAY-screened peptide P785 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGAGCACGTGGTATTATAATAGCACTATTAGGGCCAATTATATTATTGATAATACTAGGTAA GSTWYYNSTIRANYIIDNTR* -2.372 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22436 RDKFYLRGNPNGMNMTVTIC 20 SLAY-screened peptide P786 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGATAAGTTTTATCTGCGTGGTAATCCCAATGGGATGAACATGACGGTTACTATTTGTTAA RDKFYLRGNPNGMNMTVTIC* -2.371 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22437 TGDPYCHSLPITPTHNMCDL 20 SLAY-screened peptide P787 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGGTGATCCCTACTGTCATTCCCTGCCTATTACTCCGACCCATAATATGTGTGATCTTTAA TGDPYCHSLPITPTHNMCDL* -2.37 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22438 VCYCSNRSEWPSHIYVHETH 20 SLAY-screened peptide P788 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTGCTATTGTTCTAATCGCAGTGAGTGGCCTTCCCACATTTATGTTCACGAGACTCACTAA VCYCSNRSEWPSHIYVHETH* -2.37 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22439 CHRAPPISYIHYSFHLYS 18 SLAY-screened peptide P789 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCATCGTGCTCCCCCTATTTCCTATATTCACTATTCTTTTCACCTGTACTCCTAGAGTTAA CHRAPPISYIHYSFHLYS*S* -2.368 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22440 LNFSCARTNSLAPLILRT 18 SLAY-screened peptide P790 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAATTTTTCCTGTGCCCGTACCAACTCGCTTGCTCCTTTGATTCTGCGCACTTAGTTGTAA LNFSCARTNSLAPLILRT*L* -2.367 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22441 DYFHYLTFLFSLAIIFTTSLN 21 SLAY-screened peptide P791 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTACTTTCACTATCTGACGTTCTTGTTCTCATTGGCAATTATATTCACAACCTCACTTAAC DYFHYLTFLFSLAIIFTTSLN -2.367 8.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22442 PPTLMCPSITLAFTRRRSC 19 SLAY-screened peptide P792 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCACTCTTATGTGTCCCTCGATTACTTTGGCGTTCACCCGGCGCCGGTCCTGTTAGTAA PPTLMCPSITLAFTRRRSC** -2.364 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22443 TLIHIVIYGPMVLLGLCTSKS 21 SLAY-screened peptide P793 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTTATCCACATTGTGATCTATGGCCCCATGGTCCTGTTGGGCCTTTGTACGAGTAAGTCG TLIHIVIYGPMVLLGLCTSKS -2.364 0.000356 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22444 TVVLPLSVAIVYSAYSAPHL 20 SLAY-screened peptide P794 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTCGTCCTGCCCCTGTCTGTTGCCATTGTTTATTCCGCTTACTCGGCCCCTCACCTCTAA TVVLPLSVAIVYSAYSAPHL* -2.363 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22445 QRRVLVAGNSSCAIQTLRCA 20 SLAY-screened peptide P795 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCGGCGTGTTCTGGTGGCCGGTAATTCCAGCTGCGCGATCCAGACGCTGCGTTGTGCGTAA QRRVLVAGNSSCAIQTLRCA* -2.363 0.000212 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22446 APVAHNPRSGRNPAKTPTNT 20 SLAY-screened peptide P796 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCGGTTGCGCACAATCCGCGTTCGGGTCGTAACCCGGCCAAGACGCCCACCAACACTTAA APVAHNPRSGRNPAKTPTNT* -2.362 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22447 HLGITVDNCTAYAIDVLPNT 20 SLAY-screened peptide P797 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTTGGGTATTACTGTTGACAATTGCACGGCGTACGCCATCGATGTGCTTCCTAACACCTAA HLGITVDNCTAYAIDVLPNT* -2.361 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22448 TLTA 4 SLAY-screened peptide P798 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTTACGGCTTAGGTGCTCACTTAGAGTCAGAACATTCTCCTCACTTATTAGGCGTGCTAA TLTA*VLT*SQNILLTY*AC* -2.361 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22449 DPNVSTFSHCRHRLAFFSDSH 21 SLAY-screened peptide P799 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCCTAATGTTTCGACTTTCTCCCACTGCCGCCACCGACTGGCATTCTTCTCAGATTCACAT DPNVSTFSHCRHRLAFFSDSH -2.36 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22450 SSTKRPDSDDHDVSLQDNTH 20 SLAY-screened peptide P800 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCAGTACTAAGCGCCCTGACTCTGATGATCATGACGTGTCTCTCCAGGATAACACTCACTAA SSTKRPDSDDHDVSLQDNTH* -2.36 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22451 AGVTIILLPGAATFVIRRRRN 21 SLAY-screened peptide P801 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGGAGTTACCATCATCTTACTGCCTGGCGCCGCGACGTTTGTTATAAGACGACGCCGTAAC AGVTIILLPGAATFVIRRRRN -2.36 0.000311 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22452 PRWGPAWNIFRLFYKSRHPS 20 SLAY-screened peptide P802 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCGCTGGGGCCCCGCGTGGAACATCTTCCGGCTCTTCTATAAGTCTCGTCACCCCTCGTAA PRWGPAWNIFRLFYKSRHPS* -2.359 1.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22453 HLHMATYINNHYSTYFFTFF 20 SLAY-screened peptide P803 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCTTCATATGGCTACGTATATCAACAATCATTACAGTACCTATTTTTTTACCTTCTTCTAA HLHMATYINNHYSTYFFTFF* -2.358 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22454 NYSGR 5 SLAY-screened peptide P804 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTACAGCGGCCGCTAGGCTGCTGCTTTTCTTGACACCTGTGCCACTTATCCGATTGCGTAA NYSGR*AAAFLDTCATYPIA* -2.357 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22455 PPTHLSTRMCVSSATHADSR 20 SLAY-screened peptide P805 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCGACTCATCTTAGTACTCGTATGTGCGTGTCGAGCGCCACCCACGCTGATTCGAGGTAA PPTHLSTRMCVSSATHADSR* -2.355 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22456 NFYKPEFLQDYLTSYYYVLA 20 SLAY-screened peptide P806 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTTCTACAAGCCTGAGTTTCTCCAGGACTATCTTACGAGTTATTATTATGTCCTTGCCTAA NFYKPEFLQDYLTSYYYVLA* -2.355 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22457 YADTSGPALDHSNCSVCGCM 20 SLAY-screened peptide P807 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCCGACACCTCCGGCCCCGCCTTGGACCACAGCAACTGTAGTGTTTGCGGCTGCATGTAA YADTSGPALDHSNCSVCGCM* -2.354 2.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22458 VPPDTIVRDDSVALLTTLRH 20 SLAY-screened peptide P808 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCCGCCTGATACGATTGTCCGTGATGATTCGGTGGCCCTGCTTACTACCCTTAGGCACTAA VPPDTIVRDDSVALLTTLRH* -2.353 2.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22459 PRKLILILKSYRQKKTRKNS 20 SLAY-screened peptide P809 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGCAAGCTGATTCTCATCCTTAAGAGTTACCGCCAGAAGAAGACTAGGAAGAATAGCTAA PRKLILILKSYRQKKTRKNS* -2.353 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22460 PPDNRDTSWPAQERGPADHY 20 SLAY-screened peptide P810 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCGATAACCGGGATACCTCTTGGCCTGCTCAGGAGAGGGGTCCGGCGGACCATTATTAA PPDNRDTSWPAQERGPADHY* -2.351 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22461 FQSAESSPSVAIHIVDDLIH 20 SLAY-screened peptide P811 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCAGTCTGCGGAGTCGAGTCCTAGCGTGGCTATCCATATCGTCGATGATCTGATTCATTAA FQSAESSPSVAIHIVDDLIH* -2.35 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22462 YAHAPYEDDVPPQHGVVTE 19 SLAY-screened peptide P812 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCTCACGCCCCATATGAGGACGACGTGCCGCCACAACACGGAGTGGTAACTGAGTAAGTC YAHAPYEDDVPPQHGVVTE*V -2.349 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22463 VDREFLLDYKSYAKKYSLIV 20 SLAY-screened peptide P813 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGACCGTGAGTTTCTGCTTGACTACAAGTCCTACGCGAAGAAGTACTCGCTGATTGTTTAA VDREFLLDYKSYAKKYSLIV* -2.348 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22464 WKNQHGYPSHCSNMEFGNHDN 21 SLAY-screened peptide P814 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGAAGAATCAGCACGGCTACCCCTCCCATTGCAGTAATATGGAGTTTGGCAATCATGATAAC WKNQHGYPSHCSNMEFGNHDN -2.348 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22465 PICPHMHRDWIMFNNTSWIS 20 SLAY-screened peptide P815 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCATTTGCCCCCACATGCATAGGGATTGGATCATGTTCAATAATACCTCTTGGATTAGCTAA PICPHMHRDWIMFNNTSWIS* -2.348 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22466 RGHHDSYSVRCYLSPDPDHP 20 SLAY-screened peptide P816 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGGTCACCACGATTCTTATTCCGTTCGTTGTTACCTCTCTCCCGATCCTGACCACCCGTAA RGHHDSYSVRCYLSPDPDHP* -2.347 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22467 FYQVPLQNHRLDMHRPHNNN 20 SLAY-screened peptide P817 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTACCAGGTTCCTCTGCAGAACCACCGTCTGGACATGCATCGTCCGCATAATAATAATTAA FYQVPLQNHRLDMHRPHNNN* -2.346 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22468 WHS 3 SLAY-screened peptide P818 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCACTCCTAGAAGACCTATCCCAGTTTGTCGATCCTTCTGCTCTAGACCGCGACGTTCTAA WHS*KTYPSLSILLL*TATF* -2.346 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22469 YRVQPDDNVLQVRVTVAIIFN 21 SLAY-screened peptide P819 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCGTGTGCAGCCCGATGACAACGTCCTGCAGGTTAGGGTTACAGTTGCGATTATATTTAAC YRVQPDDNVLQVRVTVAIIFN -2.345 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22470 RWFAAHDFGHDCNKPIPTDT 20 SLAY-screened peptide P820 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTGGTTCGCGGCGCACGACTTTGGTCATGATTGTAACAAGCCGATTCCTACTGATACTTAA RWFAAHDFGHDCNKPIPTDT* -2.344 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22471 RRSYPLLLYHFNFKCS 16 SLAY-screened peptide P821 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCGCAGTTACCCTCTTTTGCTTTATCACTTTAACTTTAAGTGCTCTTAGGGGACCGACTAA RRSYPLLLYHFNFKCS*GTD* -2.344 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22472 LRRQPAAYMLYRTHMFLI 18 SLAY-screened peptide P822 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAGGAGGCAGCCCGCTGCGTATATGCTCTACCGTACGCATATGTTTCTCATTTAGAACTAA LRRQPAAYMLYRTHMFLI*N* -2.344 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22473 DRGTRHTSPDSYAFTSAINT 20 SLAY-screened peptide P823 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCGTGGCACGCGCCATACGTCGCCCGACTCGTATGCCTTTACTTCTGCCATTAACACCTAA DRGTRHTSPDSYAFTSAINT* -2.343 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22474 LNPCTSAVIPFDYIYIQFYE 20 SLAY-screened peptide P824 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAACCCGTGTACGAGTGCTGTCATTCCTTTCGATTACATTTATATTCAGTTTTACGAGTAA LNPCTSAVIPFDYIYIQFYE* -2.342 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22475 CCMSLVLVSSKCLCFIV 17 SLAY-screened peptide P825 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTGCATGTCTCTGGTTTTGGTTTCTTCTAAGTGCCTCTGTTTCATCGTGTAGGCTTCCTAA CCMSLVLVSSKCLCFIV*AS* -2.341 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22476 SSYFLERPRD 10 SLAY-screened peptide P826 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCTTATTTTTTGGAGCGTCCTCGTGACTAGTCGGACTACGTGAATATTATGCACATGTAA SSYFLERPRD*SDYVNIMHM* -2.34 0.000343 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22477 YVSPQGIPLRSWRFFNIVPV 20 SLAY-screened peptide P827 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGTTAGCCCTCAGGGTATTCCCCTTAGGAGCTGGCGCTTTTTTAACATTGTTCCCGTGTAA YVSPQGIPLRSWRFFNIVPV* -2.339 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22478 RAANLPVGPHLIKRSAPYLH 20 SLAY-screened peptide P828 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGCCGCCAACCTTCCCGTGGGCCCCCACCTCATTAAGCGTAGCGCGCCCTACTTGCATTAA RAANLPVGPHLIKRSAPYLH* -2.338 0.000955 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22479 FNSEHQVSRGSVVVIFVLDN 20 SLAY-screened peptide P829 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCAATTCCGAGCATCAGGTGTCTCGGGGTAGCGTGGTGGTTATTTTCGTTCTGGACAACTAA FNSEHQVSRGSVVVIFVLDN* -2.336 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22480 PPCPVLLAFPLALARVSPIN 20 SLAY-screened peptide P830 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCGTGCCCTGTACTTCTGGCATTCCCTCTCGCACTAGCGCGGGTATCACCCATTAACTGA PPCPVLLAFPLALARVSPIN* -2.335 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22481 SSCRVLSSD 9 SLAY-screened peptide P831 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTCCTGCCGTGTCCTTTCCTCTGACTAGTTTCGCTCCTGCCCCGAGTGCCAGTCCTTCTAA SSCRVLSSD*FRSCPECQSF* -2.334 0.000119 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22482 ITWALFLWYGMPNFDIDRSE 20 SLAY-screened peptide P832 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCACCTGGGCCCTTTTCCTCTGGTACGGTATGCCCAATTTTGACATTGATAGGTCGGAGTAA ITWALFLWYGMPNFDIDRSE* -2.333 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22483 IDIPDYDYQSLC 12 SLAY-screened peptide P833 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGACATTCCCGATTATGATTATCAGAGCCTTTGTTAGCTTTACCCCACTATTTATTGGTAA IDIPDYDYQSLC*LYPTIYW* -2.332 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22484 YSHMRRSHHDHVRPSDFLSS 20 SLAY-screened peptide P834 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACAGCCACATGCGTCGTTCTCATCATGATCACGTGCGGCCCAGCGATTTCCTGTCTTCTTAA YSHMRRSHHDHVRPSDFLSS* -2.332 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22485 HYPAHSYSVQIMWRNIANLP 20 SLAY-screened peptide P835 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTACCCTGCCCATTCGTACAGTGTTCAGATTATGTGGCGCAATATTGCGAATCTTCCGTAA HYPAHSYSVQIMWRNIANLP* -2.332 2.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22486 VPNSTATLNAYW 12 SLAY-screened peptide P836 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCCCAATTCTACCGCTACGCTCAACGCTTATTGGTAGTGCAGGAATGGCCTTAACTCCTAA VPNSTATLNAYW*CRNGLNS* -2.331 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22487 EDYTFLLVFVHCFVRFKSKST 21 SLAY-screened peptide P837 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGACTATACTTTTCTCCTTGTTTTCGTCCATTGCTTCGTGCGCTTCAAGAGTAAGTCGACC EDYTFLLVFVHCFVRFKSKST -2.33 0.000361 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22488 RNGFNREYCSSHVCIFNSVNN 21 SLAY-screened peptide P838 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGAATGGCTTTAACCGTGAGTATTGCTCTTCGCACGTTTGTATCTTTAATAGTGTGAATAAC RNGFNREYCSSHVCIFNSVNN -2.328 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22489 YLDVSSNIYDREYLLLCTCS 20 SLAY-screened peptide P839 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCTTGACGTTTCCAGTAATATTTATGACCGGGAGTACCTCTTGCTTTGCACGTGTTCGTAA YLDVSSNIYDREYLLLCTCS* -2.327 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22490 SHV 3 SLAY-screened peptide P840 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCATGTCTAGAGCCATTGTCAGTATTATGTGGTCCTCACGCTTGATAGCCTTACTGTGTAA SHV*SHCQYYVVLTLDSLTV* -2.327 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22491 NLEMSALPLPLYLVRLLAVN 20 SLAY-screened peptide P841 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTTGGAGATGTCTGCGTTGCCTCTGCCTCTCTACTTGGTCCGTTTGTTGGCCGTGAATTAA NLEMSALPLPLYLVRLLAVN* -2.327 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22492 PPSRPSLHNSSWFPLMYECL 20 SLAY-screened peptide P842 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCGTCTCGCCCTTCTCTGCATAATTCTTCGTGGTTCCCGCTTATGTACGAGTGCCTTTAA PPSRPSLHNSSWFPLMYECL* -2.327 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22493 TTFNLYARHQARLNTHENAP 20 SLAY-screened peptide P843 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCACTTTTAATCTGTATGCCCGTCATCAGGCTCGGCTTAACACTCATGAGAATGCTCCTTAA TTFNLYARHQARLNTHENAP* -2.326 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22494 IRRCAFAFNVLAQNQNKMTT 20 SLAY-screened peptide P844 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAGGCGTTGCGCGTTCGCTTTTAACGTGTTGGCGCAGAATCAGAACAAGATGACCACTTAA IRRCAFAFNVLAQNQNKMTT* -2.326 7.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22495 HYPNSPLPRFLNIILHRCLIN 21 SLAY-screened peptide P845 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTATCCTAACTCGCCTCTCCCCCGTTTTCTCAACATTATTCTCCATAGGTGCTTGATTAAC HYPNSPLPRFLNIILHRCLIN -2.325 0.000984 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22496 HITRCAPDWAYVLLHPH 17 SLAY-screened peptide P846 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATCACCCGCTGCGCCCCGGACTGGGCTTACGTCCTGCTTCATCCTCATTAGGGGAGCTAA HITRCAPDWAYVLLHPH*GS* -2.325 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22497 ELTPALSRYVALIETYNSAP 20 SLAY-screened peptide P847 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCTCACCCCGGCGCTCTCGCGTTATGTCGCTCTCATCGAGACGTATAACTCGGCCCCGTAA ELTPALSRYVALIETYNSAP* -2.324 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22498 HAANSACRHPMQIPVPDIIY 20 SLAY-screened peptide P848 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGCTGCTAACTCTGCTTGTCGCCACCCTATGCAGATTCCTGTCCCCGACATTATCTACTAA HAANSACRHPMQIPVPDIIY* -2.324 0.000669 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22499 RSGAHLLLTHRRGRACGLSV 20 SLAY-screened peptide P849 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTCCGGCGCGCATCTTCTCCTCACTCACCGCCGTGGGCGCGCTTGTGGCCTGTCCGTGTAA RSGAHLLLTHRRGRACGLSV* -2.323 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22500 YQFINMCSVPFHPPC 15 SLAY-screened peptide P850 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCAGTTTATTAACATGTGCTCTGTGCCTTTTCATCCTCCTTGCTAGAGCACCAGCGGTTAA YQFINMCSVPFHPPC*STSG* -2.323 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22501 LHSPHSAPHFARPASRNIDT 20 SLAY-screened peptide P851 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCATAGTCCGCATTCCGCTCCCCATTTTGCTAGGCCTGCTAGCAGGAACATTGATACGTAA LHSPHSAPHFARPASRNIDT* -2.322 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22502 CQKMAISWLQIVAILLVSTF 20 SLAY-screened peptide P852 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCAGAAGATGGCGATTAGTTGGCTTCAGATTGTCGCCATTTTGCTGGTCTCTACTTTCTAA CQKMAISWLQIVAILLVSTF* -2.322 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22503 WNQKRLRHRHTPSCSDENYL 20 SLAY-screened peptide P853 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGAACCAGAAGCGTCTCCGTCATCGTCATACTCCGTCTTGCTCCGACGAGAATTATCTGTAA WNQKRLRHRHTPSCSDENYL* -2.319 0.000663 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22504 GGEPAPLSDCACPSCAVCDR 20 SLAY-screened peptide P854 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGGTGAGCCTGCGCCGCTCTCGGATTGCGCCTGTCCTTCTTGTGCGGTGTGTGATCGGTAA GGEPAPLSDCACPSCAVCDR* -2.319 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22505 NFLLFDRNCHYPLTFGPVLG 20 SLAY-screened peptide P855 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTTCCTTTTGTTTGATAGGAATTGTCATTATCCCTTGACCTTTGGTCCGGTTCTTGGTTAA NFLLFDRNCHYPLTFGPVLG* -2.319 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22506 NTYTFMLYGLGHTLLAPRAGN 21 SLAY-screened peptide P856 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATACCTACACCTTCATGCTTTACGGCCTGGGTCACACTCTGCTGGCGCCCCGCGCCGGTAAC NTYTFMLYGLGHTLLAPRAGN -2.319 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22507 PPLDTVTHAICEIKYFRQSL 20 SLAY-screened peptide P857 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCCTTGGACACTGTCACGCACGCCATCTGCGAGATCAAGTACTTTCGCCAGTCGCTCTAA PPLDTVTHAICEIKYFRQSL* -2.318 0.001013 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22508 GPPVLSFVAINVDAPITTTTN 21 SLAY-screened peptide P858 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCCCCCTGTCCTGTCCTTTGTGGCCATTAATGTTGACGCCCCTATCACTACAACTACTAAC GPPVLSFVAINVDAPITTTTN -2.317 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22509 VTTFTNYYNRAVTLIHLT 18 SLAY-screened peptide P859 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTACGACCTTTACGAATTATTATAATCGTGCCGTCACTCTGATTCACCTGACTTAGCTTTAA VTTFTNYYNRAVTLIHLT*L* -2.316 8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22510 YLRYRTDPNYFHSYNSFCSD 20 SLAY-screened peptide P860 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCTTAGGTATCGCACTGATCCTAATTATTTCCATAGTTATAATAGCTTTTGCAGTGACTAA YLRYRTDPNYFHSYNSFCSD* -2.316 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22511 SSEKCYATFLAARYHIFN 18 SLAY-screened peptide P861 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTCCGAGAAGTGTTATGCTACTTTTCTGGCTGCGCGCTATCATATTTTTAATTAGAACTAA SSEKCYATFLAARYHIFN*N* -2.315 0.000645 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22512 VATFVLCLYRTNIQSFLHWD 20 SLAY-screened peptide P862 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGCCACTTTTGTTCTTTGCCTCTATCGCACGAACATTCAGAGCTTTCTTCACTGGGATTAA VATFVLCLYRTNIQSFLHWD* -2.315 2.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22513 RCYYPGHIIFKGYVMYNNST 20 SLAY-screened peptide P863 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTGTTATTACCCGGGGCATATTATCTTTAAGGGGTACGTCATGTATAATAATTCCACGTAA RCYYPGHIIFKGYVMYNNST* -2.314 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22514 APSRCAFHGT 10 SLAY-screened peptide P864 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCCGTCTAGGTGTGCTTTCCACGGTACTTAGGACCATAGTTTTCATAGTTCTTGCACTTAA APSRCAFHGT*DHSFHSSCT* -2.313 0.000646 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22515 LHFLVNTYCTQHVVDLRNMH 20 SLAY-screened peptide P865 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACTTCCTTGTTAACACCTATTGCACTCAGCACGTCGTTGATTTGCGTAATATGCACTAA LHFLVNTYCTQHVVDLRNMH* -2.312 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22516 VYGILLLLRRRPWCRPLLTCN 21 SLAY-screened peptide P866 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTACGGTATACTCCTCTTATTAAGAAGAAGGCCATGGTGCCGACCCTTACTAACCTGTAAC VYGILLLLRRRPWCRPLLTCN -2.312 0.001166 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22517 IAIPTSYTNVTPRAHSMASL 20 SLAY-screened peptide P867 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGCTATCCCCACCAGCTACACGAACGTTACGCCTCGCGCTCACAGCATGGCTTCGCTTTAA IAIPTSYTNVTPRAHSMASL* -2.312 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22518 PMSSTVRLRTTNDDINSCTN 20 SLAY-screened peptide P868 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATGTCTAGTACGGTCCGCCTCCGGACTACCAATGATGACATTAATTCGTGTACTAATTAA PMSSTVRLRTTNDDINSCTN* -2.311 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22519 INTKHGGYRLVCNNLSVHSG 20 SLAY-screened peptide P869 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAACACCAAGCACGGTGGCTATCGCCTGGTCTGCAACAATCTGTCTGTCCATTCCGGTTAA INTKHGGYRLVCNNLSVHSG* -2.311 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22520 LRCAIFWPIILIPP 14 SLAY-screened peptide P870 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCGGTGCGCCATCTTCTGGCCTATCATTCTCATTCCTCCTTAGGTCTGTGATAAGGCCTAA LRCAIFWPIILIPP*VCDKA* -2.311 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22521 NTNAMPVSWCSHGCSCGMD 19 SLAY-screened peptide P871 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACACGAACGCCATGCCTGTTAGCTGGTGTTCTCACGGTTGCAGTTGCGGCATGGATTAGTAA NTNAMPVSWCSHGCSCGMD** -2.31 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22522 AQADHSNVV 9 SLAY-screened peptide P872 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCAGGCGGATCATTCTAACGTTGTTTAGAATTGCTGTGATAGTCTTGTTTAGTCGTATTAA AQADHSNVV*NCCDSLV*SY* -2.308 0.001745 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22523 DVWSRWCYPLTFANADLLPI 20 SLAY-screened peptide P873 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGTTTGGTCCCGCTGGTGCTATCCTTTGACGTTTGCTAATGCCGACTTGCTCCCTATTTAA DVWSRWCYPLTFANADLLPI* -2.308 0.002886 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22524 SFILHSIAVSPGISAPDFRY 20 SLAY-screened peptide P874 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTTTATCCTCCATTCGATCGCCGTGTCCCCTGGCATCAGCGCTCCGGACTTTCGTTATTAA SFILHSIAVSPGISAPDFRY* -2.307 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22525 YAGPMHIYHNYHCRYSTCSL 20 SLAY-screened peptide P875 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCGGGCCCGATGCACATTTACCATAACTATCACTGCCGGTATTCGACCTGCAGCCTTTAA YAGPMHIYHNYHCRYSTCSL* -2.307 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22526 SHRSHENIPMGIVDSDIIDI 20 SLAY-screened peptide P876 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCATCGGTCCCATGAGAATATTCCGATGGGTATTGTTGACTCCGACATCATTGATATTTAA SHRSHENIPMGIVDSDIIDI* -2.305 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22527 PTPAIYHLRYNRPGEMQPSA 20 SLAY-screened peptide P877 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACGCCTGCTATTTACCACCTTAGGTATAACCGTCCTGGTGAGATGCAGCCCTCGGCCTAA PTPAIYHLRYNRPGEMQPSA* -2.305 0.000228 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22528 RTSWGPCTNNRSAND 15 SLAY-screened peptide P878 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACTAGCTGGGGCCCTTGTACTAACAATCGCTCTGCGAATGACTAGTATTATCCTAATTAA RTSWGPCTNNRSAND*YYPN* -2.304 6.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22529 NHHAPCISVL 10 SLAY-screened peptide P879 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCACCATGCGCCCTGCATCTCTGTCCTGTAGCGGATGGCTCTTACCATGGACGGTGCGTAA NHHAPCISVL*RMALTMDGA* -2.303 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22530 LHDNVTDPLFCSRTPSTKAA 20 SLAY-screened peptide P880 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGCACGATAACGTGACTGACCCGCTCTTTTGTAGCCGTACCCCGAGTACGAAGGCTGCCTAA LHDNVTDPLFCSRTPSTKAA* -2.303 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22531 SMVPRRHYDPGSKSLTSFWS 20 SLAY-screened peptide P881 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTATGGTCCCTCGTAGGCACTACGACCCGGGCTCTAAGTCCCTTACCAGTTTCTGGAGTTAA SMVPRRHYDPGSKSLTSFWS* -2.303 0.000146 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22532 TMTTHRLSLCHRNCHRRHLSR 21 SLAY-screened peptide P882 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTATGACGACTCATCGTCTCTCTTTGTGCCACCGCAATTGCCACCGGCGTCATTTAAGTCGA TMTTHRLSLCHRNCHRRHLSR -2.303 2.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22533 ACQSASLYQIHRDEALTLKN 20 SLAY-screened peptide P883 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCTGTCAGTCGGCCAGTCTGTACCAGATCCATAGGGACGAGGCTCTCACCCTCAAGAATTAA ACQSASLYQIHRDEALTLKN* -2.302 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22534 MHPRHKQYWFHLLLLHIANT 20 SLAY-screened peptide P884 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCACCCCAGGCACAAGCAGTACTGGTTTCATCTTTTGCTCTTGCATATTGCTAATACTTAA MHPRHKQYWFHLLLLHIANT* -2.302 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22535 TLHRYHYRQINMVSILSSHV 20 SLAY-screened peptide P885 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCTCCATCGTTATCACTACCGTCAGATTAACATGGTCTCTATTCTGAGTTCTCATGTGTAA TLHRYHYRQINMVSILSSHV* -2.3 0.000131 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22536 PQAEISYSLCILRAR 15 SLAY-screened peptide P886 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCAGGCGGAGATCTCCTATAGCCTTTGCATTCTTCGGGCTCGTTAGTGGTTGTTCAATTAA PQAEISYSLCILRAR*WLFN* -2.299 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22537 VCTYAHPYRRYWIPYVYMRL 20 SLAY-screened peptide P887 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTGTACTTACGCTCACCCCTATCGGCGCTACTGGATTCCTTATGTCTATATGCGCTTGTAA VCTYAHPYRRYWIPYVYMRL* -2.296 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22538 RRSAVRVRHPPGISTYYRLP 20 SLAY-screened peptide P888 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCGCAGCGCTGTTAGGGTTCGGCATCCTCCGGGGATTAGCACGTATTATCGGCTTCCCTAA RRSAVRVRHPPGISTYYRLP* -2.295 5.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22539 PQINFIPLPLLLVSRVFPWVN 21 SLAY-screened peptide P889 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCAGATTAACTTCATTCCGTTACCCCTACTGTTGGTTAGCCGTGTTTTCCCCTGGGTTAAC PQINFIPLPLLLVSRVFPWVN -2.295 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22540 AVGLPSYMFHPVMPNLRNHN 20 SLAY-screened peptide P890 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGTGGGGCTTCCCTCTTACATGTTCCATCCCGTTATGCCCAATCTTAGGAATCACAACTAA AVGLPSYMFHPVMPNLRNHN* -2.294 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22541 HDQRAVVTSLSFLLLVLSSV 20 SLAY-screened peptide P891 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGATCAGCGCGCGGTTGTCACTAGTCTTAGCTTCCTGTTGCTCGTTCTGTCCTCCGTTTAA HDQRAVVTSLSFLLLVLSSV* -2.293 0.000637 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22542 FRPLQAEAYNY 11 SLAY-screened peptide P892 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCGGCCCTTGCAGGCTGAGGCTTACAACTATTAGAACGTTGCGTGCATTTTTAGTCAGTAA FRPLQAEAYNY*NVACIFSQ* -2.291 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22543 PFRSNTNKYTMCSGESFSNS 20 SLAY-screened peptide P893 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTCCGCAGTAATACGAATAAGTATACTATGTGTTCCGGCGAGAGCTTTTCGAATAGTTAA PFRSNTNKYTMCSGESFSNS* -2.29 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22544 VWHPPLRAEPFTPSPDNHSA 20 SLAY-screened peptide P894 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTGGCACCCCCCGCTTCGGGCCGAGCCGTTCACTCCGTCTCCTGATAACCATTCGGCGTAA VWHPPLRAEPFTPSPDNHSA* -2.289 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22545 CPWYIDSSSAGHYFRLTSLM 20 SLAY-screened peptide P895 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCCTGGTACATCGACTCCAGTTCGGCCGGTCACTACTTTAGGCTTACGTCGCTGATGTAA CPWYIDSSSAGHYFRLTSLM* -2.289 0.002115 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22546 RSTFHIGDGNNNHRDKSHYL 20 SLAY-screened peptide P896 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTCGACGTTTCACATCGGCGATGGCAATAATAACCACCGTGATAAGTCTCATTATTTGTAA RSTFHIGDGNNNHRDKSHYL* -2.287 0.000187 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22547 PLFIVMLWVRRLKNAYAPML 20 SLAY-screened peptide P897 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTCTTTATTGTCATGCTGTGGGTTCGCCGTCTTAAGAATGCCTATGCCCCTATGCTGTAA PLFIVMLWVRRLKNAYAPML* -2.287 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22548 LTSAKGTHCEFYPAPYQGIV 20 SLAY-screened peptide P898 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACGAGCGCCAAGGGTACTCATTGTGAGTTTTATCCGGCCCCCTATCAGGGTATTGTCTAA LTSAKGTHCEFYPAPYQGIV* -2.287 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22549 NAKVWNRNI 9 SLAY-screened peptide P899 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGCGAAGGTGTGGAATAGGAATATTTAGTAGGACAATTGTCCTAGTAGTAGGGATGACTAA NAKVWNRNI**DNCPSSRDD* -2.286 0.001886 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22550 DAPLLSTPFVSNAHSRCTNS 20 SLAY-screened peptide P900 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATGCGCCTCTTCTCTCTACCCCGTTTGTGTCCAACGCTCACAGTCGGTGTACTAACTCTTAA DAPLLSTPFVSNAHSRCTNS* -2.286 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22551 TLSRLWRHLRITFTLHIDRL 20 SLAY-screened peptide P901 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTTGTCTAGGCTCTGGCGGCACCTTCGCATCACGTTTACGCTCCATATCGACAGGCTGTAA TLSRLWRHLRITFTLHIDRL* -2.286 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22552 HTSAAAPSHTSHTIANADES 20 SLAY-screened peptide P902 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACTAGTGCTGCTGCGCCGAGCCACACGAGCCATACTATCGCTAACGCTGACGAGAGCTAA HTSAAAPSHTSHTIANADES* -2.284 0.001094 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22553 IRHGLSSATNLRATPSVPISN 21 SLAY-screened peptide P903 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCGCCATGGTTTGTCGTCTGCCACCAATCTGAGGGCCACTCCTTCAGTTCCGATTAGTAAC IRHGLSSATNLRATPSVPISN -2.281 0.000358 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22554 PLLRPWDIQNHILGEYCNPC 20 SLAY-screened peptide P904 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTGCTGCGGCCTTGGGACATCCAGAATCACATCCTGGGTGAGTATTGCAATCCTTGTTAA PLLRPWDIQNHILGEYCNPC* -2.281 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22555 IYSKFTYSVNNIPGRSAFAA 20 SLAY-screened peptide P905 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTACAGCAAGTTTACCTACAGTGTTAACAACATCCCCGGCAGGAGTGCCTTTGCCGCTTAA IYSKFTYSVNNIPGRSAFAA* -2.28 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22556 STQNNWSLCLSSHTYNSFFT 20 SLAY-screened peptide P906 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTACTCAGAATAATTGGTCGCTCTGTTTGTCCTCCCATACTTATAACAGTTTTTTTACTTAA STQNNWSLCLSSHTYNSFFT* -2.28 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22557 CTSYPFLMPLQFLPLGQAWV 20 SLAY-screened peptide P907 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTACGAGCTATCCTTTTTTGATGCCTTTGCAGTTTCTTCCCCTCGGCCAGGCTTGGGTTTAA CTSYPFLMPLQFLPLGQAWV* -2.278 0.001043 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22558 TYRNLDTDE 9 SLAY-screened peptide P908 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTACCGTAACCTCGACACGGACGAGTAGCAGGGTAACGCGCTTCCTATTTAGCATGCGTAA TYRNLDTDE*QGNALPI*HA* -2.278 0.000172 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22559 RARSTPHNPRNIFRRKFDCL 20 SLAY-screened peptide P909 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGCGCGGTCTACCCCTCATAACCCTAGGAACATCTTTAGGCGGAAGTTTGACTGTCTGTAA RARSTPHNPRNIFRRKFDCL* -2.278 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22560 PDSKVYHDCSALYNNTYIML 20 SLAY-screened peptide P910 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGACTCCAAGGTTTACCACGATTGTTCCGCGCTGTATAATAATACGTATATCATGTTGTAA PDSKVYHDCSALYNNTYIML* -2.278 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22561 YTDVIILVARPRRSIRSTPRN 21 SLAY-screened peptide P911 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACGGACGTCATAATTCTGGTGGCCCGACCACGCAGAAGCATACGGAGCACTCCGCGTAAC YTDVIILVARPRRSIRSTPRN -2.278 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22562 VHLDSLYPVYEYHKYINLNF 20 SLAY-screened peptide P912 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCACCTTGACTCTCTCTATCCCGTTTACGAGTACCACAAGTACATTAACTTGAATTTTTAA VHLDSLYPVYEYHKYINLNF* -2.277 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22563 STSGMYNSLYSLTCRTYTYN 20 SLAY-screened peptide P913 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTACTAGTGGCATGTACAATAGTCTTTATAGCCTCACCTGCCGTACCTATACTTATAATTAA STSGMYNSLYSLTCRTYTYN* -2.275 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22564 RPDCNKYTKHLYSFQTKAIG 20 SLAY-screened peptide P914 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCTGATTGCAATAAGTACACTAAGCATCTCTACAGCTTCCAGACGAAGGCTATCGGCTAA RPDCNKYTKHLYSFQTKAIG* -2.274 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22565 KNLSFTCNPSQYRIVMLKHLT 21 SLAY-screened peptide P915 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGAATCTCTCTTTCACTTGTAACCCTAGCCAGTACCGCATCGTTATGTTGAAACATTTAACT KNLSFTCNPSQYRIVMLKHLT -2.273 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22566 SRNSFDTTSHPIPS 14 SLAY-screened peptide P916 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCGCAACTCGTTCGATACTACTTCTCATCCTATTCCGAGTTAGCGCCTGGGTATCTACTAA SRNSFDTTSHPIPS*RLGIY* -2.272 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22567 HSHPLWDNF 9 SLAY-screened peptide P917 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTCTCATCCTTTGTGGGATAATTTCTAGCCGCCTAACAACGTTTATGCGCTGCTTCACTAA HSHPLWDNF*PPNNVYALLH* -2.271 0.000317 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22568 DIKPRCYLGHLYGYLNHDFG 20 SLAY-screened peptide P918 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACATTAAGCCGCGCTGCTATCTTGGCCATCTGTACGGTTATCTTAACCATGACTTCGGGTAA DIKPRCYLGHLYGYLNHDFG* -2.27 0.000829 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22569 VS 2 SLAY-screened peptide P919 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTCTTAGGGCGTTCGCAACCTTGTTGAGAATTAGGTGTATCATGTCATGCATACCTAACTG VS*GVRNLVEN*VYHVMHT*L -2.269 0.000136 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22570 AGSSYLPHTCCRCCPHVEYV 20 SLAY-screened peptide P920 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGGCAGCTCGTACTTGCCTCACACCTGCTGCCGTTGCTGCCCCCACGTCGAGTACGTTTAA AGSSYLPHTCCRCCPHVEYV* -2.269 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22571 TFSRAPHPYSTSAAVLSLLS 20 SLAY-screened peptide P921 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTTCTCTCGTGCTCCGCACCCCTACAGCACTTCCGCTGCGGTCCTTTCCCTGCTCTCCTAA TFSRAPHPYSTSAAVLSLLS* -2.268 0.000114 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22572 LSNSTLASTLPRYTASRDKI 20 SLAY-screened peptide P922 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTCTAACAGTACCCTCGCGTCGACGCTCCCGCGTTACACTGCTTCGCGCGACAAGATCTAA LSNSTLASTLPRYTASRDKI* -2.268 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22573 YLLSLDNDTHHIVYHSGEAR 20 SLAY-screened peptide P923 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCTTCTGAGCCTTGACAATGATACTCATCATATCGTTTATCATAGTGGCGAGGCTCGGTAA YLLSLDNDTHHIVYHSGEAR* -2.268 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22574 PCPTDSWRWPLILTNVQSVV 20 SLAY-screened peptide P924 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTGCCCCACCGACAGCTGGCGGTGGCCTCTCATCTTGACCAACGTTCAGTCCGTCGTGTAA PCPTDSWRWPLILTNVQSVV* -2.266 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22575 YNRDDYFRNYHYRCLYPTLS 20 SLAY-screened peptide P925 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACAACCGTGACGATTATTTTAGGAATTACCATTACCGCTGCCTCTATCCCACGCTTAGCTAA YNRDDYFRNYHYRCLYPTLS* -2.265 0.001245 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22576 PDIIRAFNTIPDRIFGYASQ 20 SLAY-screened peptide P926 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGACATTATCCGCGCTTTCAACACTATTCCTGACCGTATTTTTGGCTATGCTTCCCAGTAA PDIIRAFNTIPDRIFGYASQ* -2.262 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22577 SCSHIPSRDFWTHSHTIQRA 20 SLAY-screened peptide P927 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTGCTCCCACATCCCCTCGCGCGACTTCTGGACCCATTCGCACACTATCCAGCGTGCCTAA SCSHIPSRDFWTHSHTIQRA* -2.262 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22578 NRFGYLDPVSSEFDIFGRRP 20 SLAY-screened peptide P928 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCGTTTTGGTTATCTTGATCCTGTCAGCAGTGAGTTTGACATCTTTGGCCGCCGTCCGTAA NRFGYLDPVSSEFDIFGRRP* -2.262 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22579 PLLQVCVPYIPWKGLYFYRC 20 SLAY-screened peptide P929 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTGCTCCAGGTTTGTGTTCCGTATATTCCTTGGAAGGGCCTGTATTTCTACCGTTGTTAA PLLQVCVPYIPWKGLYFYRC* -2.262 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22580 LLISFLPAAMTYPRSPRSAS 20 SLAY-screened peptide P930 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTTATTTCCTTCCTTCCCGCGGCGATGACCTACCCTCGCAGCCCTCGTAGTGCTTCCTAA LLISFLPAAMTYPRSPRSAS* -2.26 4.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22581 FHHLPVALWFPKPVLSLGTC 20 SLAY-screened peptide P931 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCACCATCTTCCCGTGGCCTTGTGGTTCCCTAAGCCGGTCTTGTCTCTGGGTACTTGTTAA FHHLPVALWFPKPVLSLGTC* -2.259 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22582 APNLKLAFPRHLMFTINNTC 20 SLAY-screened peptide P932 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCCAACCTTAAGTTGGCGTTTCCCCGTCATCTTATGTTCACTATTAATAACACCTGTTAA APNLKLAFPRHLMFTINNTC* -2.259 0.000342 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22583 PNKTIWITTTASRVRLGSLTN 21 SLAY-screened peptide P933 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAACAAGACTATCTGGATCACAACTACAGCCTCCCGTGTCAGATTAGGATCCCTAACTAAC PNKTIWITTTASRVRLGSLTN -2.258 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22584 PMDTDSPLTSSNRAYHLVQ 19 SLAY-screened peptide P934 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCATGGATACTGATAGTCCTCTGACTTCCTCTAACCGTGCGTACCACCTTGTTCAGTAACTG PMDTDSPLTSSNRAYHLVQ*L -2.258 0.000156 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22585 EDDSKLILSVPWFHLYRRRRN 21 SLAY-screened peptide P935 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGACGACTCCAAGCTGATCCTCAGTGTCCCGTGGTTCCATCTCTACCGGCGTAGGCGTAAC EDDSKLILSVPWFHLYRRRRN -2.258 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22586 LQALVSSSLPRTRYLHAYCV 20 SLAY-screened peptide P936 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCAGGCCCTTGTTAGTAGTTCTCTTCCGCGTACTCGTTACTTGCACGCTTATTGCGTCTAA LQALVSSSLPRTRYLHAYCV* -2.258 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22587 LSHSFTDNLFN 11 SLAY-screened peptide P937 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTCTCACAGTTTTACTGATAATTTGTTCAATTAGTTTGTTCCGACCTGTACCGGCCAGTAA LSHSFTDNLFN*FVPTCTGQ* -2.258 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22588 NPGQRSNSDQSY 12 SLAY-screened peptide P938 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCCCGGGCAGAGGTCGAACTCGGATCAGAGTTACTAGTTTTCCGATAAGGAGCACTGCTAA NPGQRSNSDQSY*FSDKEHC* -2.257 0.000438 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22589 FPNTANFVLCYKTLCLKYSD 20 SLAY-screened peptide P939 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCCTAACACTGCTAACTTTGTTTTGTGCTATAAGACGCTCTGCCTCAAGTATTCTGACTAA FPNTANFVLCYKTLCLKYSD* -2.256 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22590 TIVSVP 6 SLAY-screened peptide P940 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTATCGTTTCTGTTCCTTAGCAGGCTGTCAGTTACATTTTCGGCTATAATTAGCTTGAGTAA TIVSVP*QAVSYIFGYN*LE* -2.256 0.000182 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22591 WPLNMHHPNLIISYTSSYRL 20 SLAY-screened peptide P941 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCCTCTCAACATGCATCATCCTAATTTGATTATTAGCTACACGTCTTCGTACCGCCTCTAA WPLNMHHPNLIISYTSSYRL* -2.256 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22592 SLCHFLTNPPPESSRYT 17 SLAY-screened peptide P942 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCTTTGCCATTTTCTTACTAACCCGCCTCCTGAGTCTTCGCGTTATACGTAGCTTATGTAA SLCHFLTNPPPESSRYT*LM* -2.255 1.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22593 PSDFAATCWRYIKKARHVFL 20 SLAY-screened peptide P943 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTCTGATTTTGCTGCCACTTGCTGGCGCTACATCAAGAAGGCTCGTCATGTTTTTCTTTAA PSDFAATCWRYIKKARHVFL* -2.255 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22594 LHYLDGFYNDNTTYLMLQAP 20 SLAY-screened peptide P944 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCACTATCTGGACGGTTTCTATAATGATAATACCACTTACTTGATGCTCCAGGCTCCCTAA LHYLDGFYNDNTTYLMLQAP* -2.254 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22595 TLGRLQLPVPFIGDLAHNNM 20 SLAY-screened peptide P945 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTGGGCCGCCTGCAGCTTCCCGTCCCCTTCATTGGGGATCTTGCCCACAATAATATGTAA TLGRLQLPVPFIGDLAHNNM* -2.254 0.001787 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22596 PCPFLPPIHSCSKGLQRYNH 20 SLAY-screened peptide P946 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCCCCTTTCTGCCGCCTATCCATTCCTGCTCTAAGGGGCTTCAGAGGTATAATCATTAA PCPFLPPIHSCSKGLQRYNH* -2.254 7.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22597 PLRDHWSNLHHMSVPNWGLC 20 SLAY-screened peptide P947 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCCGGGATCACTGGTCTAACCTTCACCACATGTCTGTTCCTAATTGGGGGCTGTGTTAA PLRDHWSNLHHMSVPNWGLC* -2.251 3.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22598 AMHVLLHRASFYTSAFWHA 19 SLAY-screened peptide P948 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGATGCATGTTCTTCTCCATCGTGCTTCTTTTTATACCTCGGCCTTTTGGCACGCTTAGTAA AMHVLLHRASFYTSAFWHA** -2.251 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22599 PICHYPYPALQPLHRPYRAAL 21 SLAY-screened peptide P949 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATCTGCCATTACCCTTACCCTGCGCTTCAGCCTCTGCATCGGCCATATCGTGCCGCCCTA PICHYPYPALQPLHRPYRAAL -2.251 2.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22600 LQTWLFKAESCIIHLSHCET 20 SLAY-screened peptide P950 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCAGACGTGGCTTTTTAAGGCTGAGAGCTGTATTATTCACTTGTCCCACTGTGAGACCTAA LQTWLFKAESCIIHLSHCET* -2.25 9.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22601 SLPYLHLSLNLNSGYTGNAP 20 SLAY-screened peptide P951 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCTGCCGTACCTTCACCTGTCGCTGAACCTCAATTCCGGTTACACTGGCAACGCCCCGTAA SLPYLHLSLNLNSGYTGNAP* -2.249 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22602 LLYFLLLYNNRTPECRRGPSN 21 SLAY-screened peptide P952 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCTCTACTTCCTCCTTTTGTATAATAATAGGACTCCCGAATGTCGAAGAGGCCCCTCTAAC LLYFLLLYNNRTPECRRGPSN -2.249 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22603 GNPAYSSIHTVGLRKHKNTD 20 SLAY-screened peptide P953 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTAATCCTGCCTATAGCTCCATCCACACCGTTGGTCTGCGTAAGCATAAGAATACTGATTAA GNPAYSSIHTVGLRKHKNTD* -2.248 0.00251 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22604 TCRPFSAAYLFDDRHNIRPT 20 SLAY-screened peptide P954 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTGTCGCCCTTTCAGTGCCGCTTATCTTTTTGATGATCGGCACAATATCCGCCCTACTTAA TCRPFSAAYLFDDRHNIRPT* -2.247 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22605 PPHCRSRSGYCFRIRAY 17 SLAY-screened peptide P955 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCCCATTGTCGTAGCCGCTCGGGGTATTGCTTCCGTATTCGCGCGTACTAGATTACGTAA PPHCRSRSGYCFRIRAY*IT* -2.247 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22606 HYCAVTTPHVRCLATT 16 SLAY-screened peptide P956 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTATTGTGCTGTTACTACTCCTCATGTGCGGTGCCTGGCTACCACCTAGCCCGGCTATTAA HYCAVTTPHVRCLATT*PGY* -2.246 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22607 PDDI 4 SLAY-screened peptide P957 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGATGATATTTAGCCTCTTATGCACAATTACATTACCATTCCCTTGCCGCATCTCCATTAA PDDI*PLMHNYITIPLPHLH* -2.245 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22608 PSHDQRTILMLNLMYALQCV 20 SLAY-screened peptide P958 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTCCCATGACCAGAGGACGATCCTTATGTTGAATCTTATGTATGCTCTCCAGTGTGTGTAA PSHDQRTILMLNLMYALQCV* -2.245 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22609 SRPIYTWIYSPIPLYNAFKT 20 SLAY-screened peptide P959 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCGTCCCATCTATACTTGGATTTATAGCCCCATCCCCTTGTACAACGCGTTTAAGACCTAA SRPIYTWIYSPIPLYNAFKT* -2.244 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22610 ILYRKNFGCVAHIVGVVRAV 20 SLAY-screened peptide P960 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCTTTACCGCAAGAATTTTGGTTGTGTCGCTCACATTGTGGGTGTTGTGCGGGCTGTCTAA ILYRKNFGCVAHIVGVVRAV* -2.243 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22611 RTC 3 SLAY-screened peptide P961 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACTTGTTAGAACTCCTATTGCCGCCTTAGCCACCAGGGCCTTAATAATCTTCGTAACTAA RTC*NSYCRLSHQGLNNLRN* -2.243 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22612 NCNLESCSYDRTGLDGPD 18 SLAY-screened peptide P962 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTGTAACCTTGAGAGTTGCTCTTATGATAGAACAGGTCTAGATGGGCCTGACTGATAACTG NCNLESCSYDRTGLDGPD**L -2.242 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22613 CHTSNTSPGVKLASNPPNTD 20 SLAY-screened peptide P963 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCACACCTCTAACACTAGCCCGGGCGTTAAGTTGGCTTCTAATCCGCCGAATACCGACTAA CHTSNTSPGVKLASNPPNTD* -2.242 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22614 PDFSKTTLCGPTASETADPL 20 SLAY-screened peptide P964 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGATTTTAGTAAGACGACGCTTTGCGGTCCGACCGCTTCTGAGACTGCGGATCCCCTTTAA PDFSKTTLCGPTASETADPL* -2.242 0.000132 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22615 ACYAHTNGIHPEHNATHMCD 20 SLAY-screened peptide P965 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCTGCTACGCCCACACTAACGGTATTCATCCTGAGCATAACGCTACCCACATGTGCGATTAA ACYAHTNGIHPEHNATHMCD* -2.241 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22616 RRTNSRAFTTLLRRVALKTM 20 SLAY-screened peptide P966 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCGTACCAACTCCAGGGCTTTCACCACGCTGCTGAGGCGTGTCGCCCTTAAGACCATGTAA RRTNSRAFTTLLRRVALKTM* -2.24 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22617 SRPRLATSCSHLLTSALPSTN 21 SLAY-screened peptide P967 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCGTCCTCGGCTTGCTACCTCTTGCTCCCATCTACTTACAAGCGCACTACCTTCAACTAAC SRPRLATSCSHLLTSALPSTN -2.238 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22618 IILIFLRNRPRGC 13 SLAY-screened peptide P968 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTATTCTCATCTTCCTTCGTAACCGTCCGAGGGGGTGTTAGACGCACAGCGACTCCTGCTAA IILIFLRNRPRGC*THSDSC* -2.238 0.002889 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22619 SNRFYTMPHHTPTELEPRSP 20 SLAY-screened peptide P969 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAACCGGTTCTACACCATGCCGCACCACACTCCTACTGAGCTTGAGCCTCGTAGCCCGTAA SNRFYTMPHHTPTELEPRSP* -2.237 0.000227 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22620 RPIFLSTAYRTDYYPITYSV 20 SLAY-screened peptide P970 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCCATCTTCTTGTCCACGGCGTACCGTACTGATTATTACCCGATCACGTACTCCGTTTAA RPIFLSTAYRTDYYPITYSV* -2.236 0.002588 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22621 HFYTDIYKALRIGHNATKLH 20 SLAY-screened peptide P971 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTTTTACACTGATATTTACAAGGCTCTCCGTATCGGTCACAACGCCACTAAGCTGCACTAA HFYTDIYKALRIGHNATKLH* -2.236 4.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22622 PTVGVWSLLPYRCTNIYTRA 20 SLAY-screened peptide P972 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACCGTCGGGGTCTGGTCCCTCCTGCCGTATCGGTGCACCAATATCTACACGCGTGCTTAA PTVGVWSLLPYRCTNIYTRA* -2.235 0.003337 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22623 GHQLSNPPFSPNLRTHTYGL 20 SLAY-screened peptide P973 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCATCAGCTGTCCAATCCGCCGTTCAGCCCGAACCTTCGTACGCACACGTATGGTCTGTAA GHQLSNPPFSPNLRTHTYGL* -2.235 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22624 RLSCDYRRAACLEHNFSLFQY 21 SLAY-screened peptide P974 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTTTCGTGCGACTATCGGCGTGCCGCGTGTCTTGAGCATAACTTTTCGCTGTTCCAGTAC RLSCDYRRAACLEHNFSLFQY -2.234 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22625 RDYKLTFTYFSSHGHSR 17 SLAY-screened peptide P975 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGACTATAAGCTCACTTTTACTTACTTTTCGAGTCACGGGCATTCTCGGTAGCGTAGTTAA RDYKLTFTYFSSHGHSR*RS* -2.234 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22626 SSYLITDSDIDSVPDSFIST 20 SLAY-screened peptide P976 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCAGTTATCTTATCACTGACAGCGATATTGACTCTGTTCCTGATTCTTTCATTTCCACCTAA SSYLITDSDIDSVPDSFIST* -2.233 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22627 TQHSLHEPGTDLASSTTFL 19 SLAY-screened peptide P977 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCAGCACTCCCTCCATGAGCCCGGTACGGACCTGGCCAGTTCTACTACTTTTCTCTAGCTC TQHSLHEPGTDLASSTTFL*L -2.231 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22628 LRHPRDLVGSDTVTLHHFIS 20 SLAY-screened peptide P978 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAGGCATCCCCGTGATCTCGTTGGTTCTGATACTGTCACTCTGCATCATTTTATTTCCTAA LRHPRDLVGSDTVTLHHFIS* -2.23 0.000243 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22629 TARI 4 SLAY-screened peptide P979 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGCCCGTATTTAGATTAACCCTGACAGCAATCTTATTATCAACAGCCCCTTTTAGTACTAA TARI*INPDSNLIINSPF*Y* -2.23 0.000262 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22630 YAYSPHVYAHTFQRDYYRNL 20 SLAY-screened peptide P980 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGCCTATTCCCCTCATGTGTATGCTCATACGTTTCAGAGGGACTACTATAGGAATCTGTAA YAYSPHVYAHTFQRDYYRNL* -2.229 0.000633 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22631 ATKPLSNTMHTN 12 SLAY-screened peptide P981 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTACCAAGCCCCTTTCTAACACGATGCATACTAATTAGTTTAACAGCGTTACGGTCCAGTAA ATKPLSNTMHTN*FNSVTVQ* -2.229 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22632 LHNSTIGENFR 11 SLAY-screened peptide P982 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCATAATAGCACCATCGGCGAGAATTTTCGTTAGTTTTCCCATTATACGATGACTATGTAA LHNSTIGENFR*FSHYTMTM* -2.229 6.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22633 IARALRSYVGLFAVAQ 16 SLAY-screened peptide P983 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGCGCGGGCACTGCGCTCTTATGTGGGCCTTTTTGCAGTAGCTCAGTAGTAAGGTCTAACT IARALRSYVGLFAVAQ**GLT -2.229 0.00089 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22634 PPCAFIPMLARHQQSTRTLE 20 SLAY-screened peptide P984 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCCTGTGCGTTTATTCCGATGTTGGCTCGTCACCAGCAGTCCACTAGGACTCTGGAGTAA PPCAFIPMLARHQQSTRTLE* -2.229 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22635 PYNCKNTWSLRSHIRQALHN 20 SLAY-screened peptide P985 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTACAATTGTAAGAATACGTGGTCCTTGCGGTCGCATATCAGGCAGGCGCTCCATAACTAA PYNCKNTWSLRSHIRQALHN* -2.228 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22636 RITRRSRGYLPDRDGTVSNR 20 SLAY-screened peptide P986 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTATTACCAGGCGTTCTAGGGGTTATTTGCCTGATAGGGACGGCACGGTCTCGAACCGTTAA RITRRSRGYLPDRDGTVSNR* -2.226 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22637 YSPSDPPHYSVLPFYEIAAA 20 SLAY-screened peptide P987 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTCCCCGAGTGATCCTCCGCATTACTCGGTGCTGCCCTTTTATGAGATTGCTGCTGCTTAA YSPSDPPHYSVLPFYEIAAA* -2.225 0.000232 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22638 LPTACDYVGHHNDKTMTRLY 20 SLAY-screened peptide P988 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCCACGGCGTGCGACTATGTCGGTCACCATAACGACAAGACCATGACCCGTCTTTACTAA LPTACDYVGHHNDKTMTRLY* -2.225 0.000454 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22639 LVTW 4 SLAY-screened peptide P989 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGTTACTTGGTAGCACCACTTTTGCCACCAGTTGTCTCACCAGATTAATTATTTTTGTTAA LVTW*HHFCHQLSHQINYFC* -2.225 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22640 GASHFFPLSFDINVYLC 17 SLAY-screened peptide P990 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGGCTAGTCATTTTTTCCCTTTGTCTTTCGATATTAATGTTTATCTGTGCTAGTCGACCTAA GASHFFPLSFDINVYLC*ST* -2.224 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22641 PHRPFWFSLGHSVLSQALAF 20 SLAY-screened peptide P991 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCACCGTCCTTTTTGGTTTAGCCTGGGCCATTCTGTGCTGTCTCAGGCGCTTGCCTTTTAA PHRPFWFSLGHSVLSQALAF* -2.224 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22642 LGPSLRCMPLWQYYSI 16 SLAY-screened peptide P992 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGGTCCGTCCCTTCGCTGCATGCCCCTCTGGCAGTACTACTCTATCTAGCGGAGTTACTAA LGPSLRCMPLWQYYSI*RSY* -2.223 0.001571 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22643 RHVVLMLHTSDAYTSAPHFS 20 SLAY-screened peptide P993 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCACGTCGTGCTGATGCTGCACACCTCTGACGCGTACACTTCCGCCCCCCATTTCTCCTAA RHVVLMLHTSDAYTSAPHFS* -2.222 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22644 DPIYISS 7 SLAY-screened peptide P994 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCCGATCTATATTAGCAGTTAGACTAAGCATAGTACTATCTCCAATTACTCTAACTCTTAA DPIYISS*TKHSTISNYSNS* -2.221 0.000193 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22645 LSGIYMWCFVCINSSASPAP 20 SLAY-screened peptide P995 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCTGGTATCTACATGTGGTGTTTCGTTTGTATCAACAGCTCTGCGAGTCCGGCCCCGTAA LSGIYMWCFVCINSSASPAP* -2.22 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22646 VHLVNYGLVSRWPPDPTGRP 20 SLAY-screened peptide P996 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCACCTTGTTAATTATGGCTTGGTTTCGCGTTGGCCCCCTGATCCCACCGGTAGGCCCTAA VHLVNYGLVSRWPPDPTGRP* -2.22 0.000452 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22647 RRYLRFSGYYLHSTHNKCHY 20 SLAY-screened peptide P997 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCGCTATCTTCGCTTCTCCGGGTACTATCTGCATAGTACTCATAACAAGTGTCACTATTAA RRYLRFSGYYLHSTHNKCHY* -2.218 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22648 RPPVNSHTSST 11 SLAY-screened peptide P998 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCTCCGGTTAATTCGCACACCAGTAGCACGTAGGCTGCGCCTACCGCTTTGAACGTTTAA RPPVNSHTSST*AAPTALNV* -2.218 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22649 YTMNIDSRVENLPIP 15 SLAY-screened peptide P999 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATACCATGAATATCGACAGTAGGGTGGAGAATCTTCCGATCCCTTAGCATTCTATTACTAAC YTMNIDSRVENLPIP*HSITN -2.218 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22650 APTNYPVVYYNSTTIEKVSG 20 SLAY-screened peptide P1000 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCCGACGAACTATCCTGTTGTCTACTACAATTCGACGACCATTGAGAAGGTTTCGGGTTAA APTNYPVVYYNSTTIEKVSG* -2.217 0.003046 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22651 PTTLRIRVSPFLARCRRARRN 21 SLAY-screened peptide P1001 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACGACGTTGCGGATCAGAGTTAGTCCATTCTTGGCCCGCTGCAGACGCGCGAGACGTAAC PTTLRIRVSPFLARCRRARRN -2.216 9.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22652 RVTIYPGAATPVDKRHLTPA 20 SLAY-screened peptide P1002 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGTCACGATTTATCCTGGCGCGGCTACTCCTGTCGACAAGAGGCACCTTACCCCGGCGTAA RVTIYPGAATPVDKRHLTPA* -2.215 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22653 RRTSGLAVCNQLHFAICIYN 20 SLAY-screened peptide P1003 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCGTACCTCTGGTCTCGCGGTTTGCAACCAGCTGCACTTTGCTATTTGTATTTATAATTAA RRTSGLAVCNQLHFAICIYN* -2.215 0.000199 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22654 PLYGNTVSYPVASLQRYANL 20 SLAY-screened peptide P1004 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTTTATGGCAATACCGTTAGCTATCCCGTGGCGAGTCTTCAGCGCTATGCTAATCTCTAA PLYGNTVSYPVASLQRYANL* -2.215 0.000123 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22655 FLDPEPKCIHNRNHNPTPSD 20 SLAY-screened peptide P1005 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTTGGACCCTGAGCCCAAGTGTATCCATAACCGGAATCATAACCCTACCCCCAGTGATTAA FLDPEPKCIHNRNHNPTPSD* -2.215 0.000449 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22656 LLIHLCRCPNLCSLVSGNNT 20 SLAY-screened peptide P1006 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCTCATTCACCTCTGCAGGTGCCCCAATCTGTGTAGCCTTGTGAGCGGTAACAATACTTAA LLIHLCRCPNLCSLVSGNNT* -2.214 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22657 DALAHAHYSPLNNSHCCYCY 20 SLAY-screened peptide P1007 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATGCCCTCGCTCACGCTCATTATTCTCCTCTGAACAACAGTCATTGTTGTTACTGCTACTAA DALAHAHYSPLNNSHCCYCY* -2.214 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22658 PLSSKRNNTHYSHTRAYKIR 20 SLAY-screened peptide P1008 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTTGTCGTCTAAGAGGAATAACACTCATTACTCTCACACGCGCGCTTATAAGATCCGTTAA PLSSKRNNTHYSHTRAYKIR* -2.213 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22659 TYIAHCSVGRLYMYYSINRS 20 SLAY-screened peptide P1009 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTACATTGCTCATTGTAGCGTTGGCCGCCTGTATATGTACTACAGCATTAATCGTTCCTAA TYIAHCSVGRLYMYYSINRS* -2.21 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22660 YFLSCPLMQANPGTVAT 17 SLAY-screened peptide P1010 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTTTCTTTCCTGTCCGCTCATGCAGGCTAATCCGGGTACCGTCGCGACCTAGGGGCACTAA YFLSCPLMQANPGTVAT*GH* -2.208 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22661 PHCDSTIYIRHYPRDNMALT 20 SLAY-screened peptide P1011 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCATTGTGACTCGACCATTTACATCAGGCATTATCCTCGCGATAATATGGCTCTGACCTAA PHCDSTIYIRHYPRDNMALT* -2.208 1.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22662 QHTHSRKADTCITPMPCSAD 20 SLAY-screened peptide P1012 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCATACTCACTCTCGTAAGGCCGATACTTGCATTACGCCCATGCCGTGTAGTGCCGATTAA QHTHSRKADTCITPMPCSAD* -2.208 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22663 TTRHSHVKTCFYTGVPLHPL 20 SLAY-screened peptide P1013 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCACTCGCCATTCGCACGTTAAGACTTGCTTCTATACTGGCGTCCCTCTTCACCCCCTCTAA TTRHSHVKTCFYTGVPLHPL* -2.208 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22664 TVLLPCGPFHVLY 13 SLAY-screened peptide P1014 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTTCTCCTTCCGTGCGGCCCTTTTCACGTTTTGTATTAGCATCCGGATGAGAATCTCTAA TVLLPCGPFHVLY*HPDENL* -2.207 5.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22665 CYSSLLLRFWLFVKALSAQF 20 SLAY-screened peptide P1015 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTATTCGTCTCTGCTTCTCCGCTTTTGGCTTTTCGTTAAGGCGCTGAGTGCCCAGTTTTAA CYSSLLLRFWLFVKALSAQF* -2.206 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22666 RPSFPGLHLPVVVSRLSCSIN 21 SLAY-screened peptide P1016 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCGTCTTTCCCTGGGTTGCATCTTCCTGTTGTCGTAAGCCGCCTGTCCTGCAGTATTAAC RPSFPGLHLPVVVSRLSCSIN -2.206 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22667 PPCLSYSATRRRYCCLPQIT 20 SLAY-screened peptide P1017 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCGTGTCTCAGTTATTCTGCTACCCGTCGGCGGTATTGCTGTTTGCCTCAGATCACTTAA PPCLSYSATRRRYCCLPQIT* -2.205 0.001147 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22668 NDFDATHLRSDRKNITYWLL 20 SLAY-screened peptide P1018 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGACTTTGATGCTACCCACCTCAGGAGTGATAGGAAGAATATTACCTACTGGCTTCTGTAA NDFDATHLRSDRKNITYWLL* -2.2 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22669 IGPLSCTPLLFIAWNSESVS 20 SLAY-screened peptide P1019 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGGCCCGCTTAGTTGTACTCCCCTCCTTTTTATCGCTTGGAACAGTGAGTCCGTTTCGTAA IGPLSCTPLLFIAWNSESVS* -2.2 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22670 NCRSVLVYYGTPGTRSYHCY 20 SLAY-screened peptide P1020 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTGCCGCAGTGTCCTTGTGTATTACGGCACTCCTGGTACTCGTAGTTATCATTGCTATTAA NCRSVLVYYGTPGTRSYHCY* -2.197 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22671 AYCVASAALAHLYVWTMTIP 20 SLAY-screened peptide P1021 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTTACTGCGTGGCGTCCGCGGCCCTCGCCCACCTTTATGTCTGGACTATGACTATCCCGTAA AYCVASAALAHLYVWTMTIP* -2.197 0.003325 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22672 IDSPAYVTLCVPRRRKQAYN 20 SLAY-screened peptide P1022 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGACAGTCCCGCCTACGTCACCCTTTGCGTTCCGCGGAGGAGGAAGCAGGCTTACAACTAA IDSPAYVTLCVPRRRKQAYN* -2.196 0.000504 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22673 TVQINSADPYCI 12 SLAY-screened peptide P1023 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTTCAGATCAATAGTGCCGACCCCTATTGCATCTAGGATGATCTGCATCATTCTGACTAA TVQINSADPYCI*DDLHHSD* -2.196 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22674 LLS 3 SLAY-screened peptide P1024 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTCTCGTAGCTGATCTTTGGTGTGAGTGGTCACGCCGCGGCTAACGGCTTCGATTCCTAA LLS*LIFGVSGHAAANGFDS* -2.196 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22675 TSATTCVYYRPRRHYCSMAS 20 SLAY-screened peptide P1025 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAGCGCCACGACTTGCGTTTATTATCGGCCGCGCAGGCACTATTGTTCGATGGCGTCCTAA TSATTCVYYRPRRHYCSMAS* -2.196 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22676 HNFGQDLAYFHQAYFNGGGG 20 SLAY-screened peptide P1026 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAACTTTGGGCAGGACTTGGCCTATTTTCATCAGGCTTATTTCAATGGCGGTGGTGGCTAA HNFGQDLAYFHQAYFNGGGG* -2.195 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22677 HGLRLPAGGTDVCDRHAPRT 20 SLAY-screened peptide P1027 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGGCCTGCGCCTGCCGGCGGGTGGGACCGATGTCTGTGACCGTCATGCTCCTCGTACGTAA HGLRLPAGGTDVCDRHAPRT* -2.195 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22678 HGFSLHFTLLDSSIYVSILN 20 SLAY-screened peptide P1028 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGGGTTCTCGCTCCATTTTACCCTTTTGGATAGCTCTATCTACGTTAGTATCCTTAATTAA HGFSLHFTLLDSSIYVSILN* -2.194 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22679 PPFDVKDVPGLLVP 14 SLAY-screened peptide P1029 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTTTCGACGTTAAGGACGTTCCGGGCCTCTTGGTCCCTTAGCGTTAGTTCTTGCACTAA PPFDVKDVPGLLVP*R*FLH* -2.194 0.00015 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22680 SHKTDQSTDDGVRDTRYSNS 20 SLAY-screened peptide P1030 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCACAAGACTGACCAGTCGACGGACGATGGTGTCAGGGATACTCGTTATTCGAATAGTTAA SHKTDQSTDDGVRDTRYSNS* -2.193 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22681 PGQLSIYLRMYRIKRTRCRV 20 SLAY-screened peptide P1031 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGGTCAGCTTTCCATCTACCTTCGCATGTATCGCATTAAGCGCACGCGCTGCCGGGTCTAA PGQLSIYLRMYRIKRTRCRV* -2.193 0.000593 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22682 PEFSLSNNSPCSTINALERL 20 SLAY-screened peptide P1032 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGAGTTTAGCCTTAGCAATAATAGCCCGTGCAGTACGATTAATGCGCTGGAGAGGTTGTAA PEFSLSNNSPCSTINALERL* -2.192 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22683 QFATFARASLIPNLPRVGPL 20 SLAY-screened peptide P1033 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTTCGCCACCTTCGCGCGGGCCTCCCTGATCCCTAATCTTCCTAGGGTCGGTCCCCTTTAA QFATFARASLIPNLPRVGPL* -2.192 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22684 HRTNYMSKYHD 11 SLAY-screened peptide P1034 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCGTACTAATTATATGTCTAAGTATCATGATTAGATGGCCACGTAGCTTTTCATCTTGTGA HRTNYMSKYHD*MAT*LFIL* -2.192 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22685 HVHQ 4 SLAY-screened peptide P1035 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGTGCACCAGTAGGTCTCCACCTATACTAATATTACGAATTAGACCGGCTGTATGCAGTAA HVHQ*VSTYTNITN*TGCMQ* -2.191 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22686 NCTRRVNATITLAGLRMLIR 20 SLAY-screened peptide P1036 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTGTACGCGGCGCGTGAATGCTACTATCACCCTGGCGGGCCTCCGGATGCTGATTCGTTAA NCTRRVNATITLAGLRMLIR* -2.189 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22687 LNNI 4 SLAY-screened peptide P1037 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAACAATATCTAGTACCCTGAGTGGCCCTGTAATATTCCCGTTCCGCGGCCGTCTAATTAA LNNI*YPEWPCNIPVPRPSN* -2.189 4.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22688 DPCQGVAVSPPFPRIAIGRAN 21 SLAY-screened peptide P1038 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCCGTGTCAGGGGGTTGCGGTGTCACCACCATTTCCGCGAATTGCAATTGGGAGGGCTAAC DPCQGVAVSPPFPRIAIGRAN -2.189 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22689 NFIRPADGHPSCVARFHDAI 20 SLAY-screened peptide P1039 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTTCATCCGTCCGGCCGATGGTCACCCGTCCTGTGTTGCCCGGTTTCATGACGCCATCTAA NFIRPADGHPSCVARFHDAI* -2.188 0.000323 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22690 LSVAPDRFVIQTLSLHTHQG 20 SLAY-screened peptide P1040 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCGGTTGCCCCCGATCGCTTTGTTATTCAGACGCTTAGCCTCCATACCCATCAGGGTTAA LSVAPDRFVIQTLSLHTHQG* -2.187 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22691 FNLILLRRLAAWRRKSYTPH 20 SLAY-screened peptide P1041 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCAACTTGATTCTGCTGCGTCGTTTGGCCGCTTGGCGCAGGAAGTCGTATACGCCTCATTAA FNLILLRRLAAWRRKSYTPH* -2.187 0.0014 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22692 PDPVNANTNRIMCLVYYNSM 20 SLAY-screened peptide P1042 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGACCCGGTCAACGCCAACACTAATCGTATTATGTGCCTTGTGTACTATAATTCTATGTAA PDPVNANTNRIMCLVYYNSM* -2.186 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22693 LAHPPPVPHNRWHSFTTYLL 20 SLAY-screened peptide P1043 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGCCCATCCCCCTCCCGTTCCCCATAACCGGTGGCATTCTTTTACTACTTATCTCCTCTAA LAHPPPVPHNRWHSFTTYLL* -2.186 0.001717 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22694 RFLVHAFHPLNPPLTLVHNE 20 SLAY-screened peptide P1044 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTTCCTTGTTCATGCGTTTCATCCGCTTAATCCTCCTTTGACGCTGGTGCACAACGAGTAA RFLVHAFHPLNPPLTLVHNE* -2.186 5.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22695 GSTCHSRHDNHILDHSFNSR 20 SLAY-screened peptide P1045 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGTCTACCTGCCACTCTCGCCATGATAATCATATCCTTGACCACAGCTTTAACTCCAGGTAA GSTCHSRHDNHILDHSFNSR* -2.186 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22696 CLDYVDTETCSPCFNRTVTSN 21 SLAY-screened peptide P1046 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTTGGATTACGTCGATACTGAGACCTGCAGTCCCTGTTTTAATAGAACAGTTACGAGTAAC CLDYVDTETCSPCFNRTVTSN -2.186 0.000408 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22697 HNLTYTFLSSCPWVYSVSYH 20 SLAY-screened peptide P1047 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAACCTTACGTATACCTTTCTCTCTTCGTGTCCTTGGGTATATAGCGTCTCGTATCACTAA HNLTYTFLSSCPWVYSVSYH* -2.185 0.002589 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22698 SPYKRLMLILIFIILISVRIN 21 SLAY-screened peptide P1048 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCCCTACAAGAGACTGATGCTTATCCTAATATTTATCATACTAATCAGCGTGCGGATTAAC SPYKRLMLILIFIILISVRIN -2.184 3.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22699 YTGRFNGHNCKCGALCLDYI 20 SLAY-screened peptide P1049 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACGGGCCGTTTTAACGGCCACAACTGTAAGTGTGGTGCTCTTTGTCTTGATTACATTTAA YTGRFNGHNCKCGALCLDYI* -2.184 0.005556 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22700 AKRGTPLICFGLFTRMDRRL 20 SLAY-screened peptide P1050 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAAGCGCGGGACTCCTCTCATCTGTTTTGGGCTTTTTACTAGGATGGATAGGCGGTTGTAA AKRGTPLICFGLFTRMDRRL* -2.183 5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22701 IGDNGYFFPSKHIRSNFPD 19 SLAY-screened peptide P1051 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGGGGACAACGGTTACTTTTTCCCTTCCAAGCATATCCGTAGTAATTTCCCTGACTAGTAA IGDNGYFFPSKHIRSNFPD** -2.183 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22702 TGQTHANAHRHNPYFSTLDV 20 SLAY-screened peptide P1052 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGGCCAGACCCACGCTAACGCTCACAGGCACAACCCCTACTTTTCCACCCTCGACGTGTAA TGQTHANAHRHNPYFSTLDV* -2.183 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22703 YSHAFMASFGHCAKSYYLVP 20 SLAY-screened peptide P1053 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTCCCATGCCTTCATGGCCTCGTTTGGCCATTGTGCTAAGAGTTACTATCTGGTCCCCTAA YSHAFMASFGHCAKSYYLVP* -2.183 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22704 QATSYARTTNFLPILLICGH 20 SLAY-screened peptide P1054 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGCTACCAGCTATGCGCGTACTACCAACTTTCTGCCCATCCTCCTCATTTGCGGCCATTAA QATSYARTTNFLPILLICGH* -2.182 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22705 PLTISSNYRNVVGESGSTSI 20 SLAY-screened peptide P1055 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTACTATTTCCTCCAATTATAGGAACGTTGTCGGGGAGTCTGGTAGTACTAGTATTTAA PLTISSNYRNVVGESGSTSI* -2.181 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22706 RGNSPQFLNSWPNRSASDIL 20 SLAY-screened peptide P1056 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGGGTAATTCTCCCCAGTTTCTCAATTCGTGGCCGAATCGTTCGGCTAGTGACATCCTCTAA RGNSPQFLNSWPNRSASDIL* -2.18 0.00051 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22707 NGQGTATPATYSNSGYNARC 20 SLAY-screened peptide P1057 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGGGCAGGGTACTGCTACGCCCGCGACTTACTCTAACTCTGGTTATAATGCTCGCTGCTAA NGQGTATPATYSNSGYNARC* -2.18 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22708 PTLLNNPQTGQSTNTSHEWQ 20 SLAY-screened peptide P1058 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGACTCTGCTTAACAACCCTCAGACCGGTCAGAGCACTAATACTTCTCATGAGTGGCAGTAA PTLLNNPQTGQSTNTSHEWQ* -2.18 0.000847 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22709 MLYPIWQPINILMA 14 SLAY-screened peptide P1059 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTTGTACCCCATCTGGCAGCCGATCAATATTCTCATGGCTTAGTAGTCTCTTAGGTATTAA MLYPIWQPINILMA**SLRY* -2.179 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22710 SALANLAQPLTTLSMIRTRAN 21 SLAY-screened peptide P1060 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGCTCTCGCGAATCTGGCTCAGCCTCTTACTACGTTGTCCATGATACGAACCAGAGCTAAC SALANLAQPLTTLSMIRTRAN -2.179 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22711 SNATAINSLAYYLFDISVAA 20 SLAY-screened peptide P1061 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGAACGCCACGGCCATCAATTCCCTGGCGTATTATCTCTTTGATATTAGCGTGGCCGCTTAA SNATAINSLAYYLFDISVAA* -2.179 1.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22712 MHPWSNAHTLATDSSSADSH 20 SLAY-screened peptide P1062 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCACCCCTGGTCTAACGCTCATACGCTTGCGACGGACAGTTCCTCTGCTGATTCTCATTAA MHPWSNAHTLATDSSSADSH* -2.179 0.001379 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22713 IYCIVFPRLYDNCSIVGMSCN 21 SLAY-screened peptide P1063 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTACTGTATCGTGTTCCCTCGCTTGTACGATAACTGTTCTATCGTCGGCATGTCTTGTAAC IYCIVFPRLYDNCSIVGMSCN -2.178 0.000492 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22714 CYRIPLC 7 SLAY-screened peptide P1064 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTATCGTATCCCTCTGTGCTAGACTACGTCTGACTCGTATAATGGCTCCTCCTCGCCTTAA CYRIPLC*TTSDSYNGSSSP* -2.178 0.000362 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22715 LGREMFFSRYNGNHSRHTSS 20 SLAY-screened peptide P1065 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGGCAGGGAGATGTTTTTTTCCCGTTACAATGGCAATCACTCCCGGCATACCTCTTCCTAA LGREMFFSRYNGNHSRHTSS* -2.178 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22716 ICSRNMHMYRRIFNDYMFCD 20 SLAY-screened peptide P1066 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTGCTCGCGCAACATGCATATGTACCGCCGTATCTTCAATGATTACATGTTCTGTGATTAA ICSRNMHMYRRIFNDYMFCD* -2.177 0.000903 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22717 RSDHLPVRPIPSHILVTEDY 20 SLAY-screened peptide P1067 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTAGCGATCACTTGCCTGTGCGCCCGATTCCTAGCCATATTTTGGTTACTGAGGACTATTAA RSDHLPVRPIPSHILVTEDY* -2.177 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22718 KHRLYTITLLDIPTSYNTLY 20 SLAY-screened peptide P1068 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCATCGTCTTTATACTATCACTCTGCTCGACATCCCGACGAGCTATAATACCCTCTACTAA KHRLYTITLLDIPTSYNTLY* -2.177 0.00129 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22719 CYPLSRSVCYFVTTYFLFSF 20 SLAY-screened peptide P1069 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTATCCTCTTTCTCGTTCTGTGTGCTATTTTGTCACCACCTATTTCCTCTTCAGTTTCTAA CYPLSRSVCYFVTTYFLFSF* -2.176 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22720 YGYDHSLPHRYYRALPVTLL 20 SLAY-screened peptide P1070 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGGCTATGACCACAGCCTCCCCCACCGGTACTACCGTGCCCTTCCGGTTACGCTCCTCTAA YGYDHSLPHRYYRALPVTLL* -2.176 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22721 GWPDSNLTMRNQSRIYCGT 19 SLAY-screened peptide P1071 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGTGGCCCGACAGTAATCTCACTATGCGTAACCAGAGCCGTATCTACTGTGGCACTTAACTG GWPDSNLTMRNQSRIYCGT*L -2.176 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22722 QTNYTVTSFSLVLTTMVMWH 20 SLAY-screened peptide P1072 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGACCAATTACACTGTCACGTCGTTTAGCCTTGTTCTCACCACGATGGTCATGTGGCACTAA QTNYTVTSFSLVLTTMVMWH* -2.175 5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22723 AGPENAAAPANFVDISLYAC 20 SLAY-screened peptide P1073 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGGGCCTGAGAATGCTGCTGCGCCGGCCAATTTTGTTGATATCTCTTTGTATGCTTGTTAA AGPENAAAPANFVDISLYAC* -2.174 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22724 TRIRIFGVCAFPATS 15 SLAY-screened peptide P1074 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCGTATTAGGATTTTTGGGGTTTGCGCGTTCCCGGCTACTAGTTAGGCGTCTCTTGCCTAA TRIRIFGVCAFPATS*ASLA* -2.173 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22725 RDFAYTPGNPFNPGLNSPRR 20 SLAY-screened peptide P1075 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGATTTTGCTTACACGCCCGGCAATCCTTTTAACCCCGGGCTCAACAGCCCTCGTCGTTAA RDFAYTPGNPFNPGLNSPRR* -2.172 0.003904 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22726 HNLCSIDNFYTIPSV 15 SLAY-screened peptide P1076 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAATCTCTGTTCCATTGATAATTTTTATACGATCCCTTCCGTCTAGGGGGGGCACTGTTAA HNLCSIDNFYTIPSV*GGHC* -2.172 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22727 HCDTTIKACSRYALR 15 SLAY-screened peptide P1077 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGTGATACGACGATTAAGGCTTGTAGTCGTTATGCTCTTCGTTAGGACCTGTTTGTCTAA HCDTTIKACSRYALR*DLFV* -2.171 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22728 NNILFTRFFPNDHGPRVGYH 20 SLAY-screened peptide P1078 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAACATCCTCTTTACTCGTTTCTTTCCTAATGATCACGGGCCCAGGGTCGGCTACCACTAA NNILFTRFFPNDHGPRVGYH* -2.171 0.003973 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22729 AATVSNCLGLYYLSHNDTSFN 21 SLAY-screened peptide P1079 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGCTACTGTGTCTAATTGTCTGGGCCTTTACTATCTGAGTCACAATGACACTTCCTTTAAC AATVSNCLGLYYLSHNDTSFN -2.17 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22730 SFSGLCEDTETILKRRTT 18 SLAY-screened peptide P1080 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTTCTCTGGCCTTTGCGAGGACACGGAGACTATTCTCAAGAGGCGCACCACTTAGAATTAA SFSGLCEDTETILKRRTT*N* -2.17 0.000319 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22731 NRACSTPTSFCSVPQVPNTY 20 SLAY-screened peptide P1081 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACAGGGCCTGTAGCACCCCTACCAGCTTCTGCTCGGTCCCGCAGGTTCCGAACACTTATTAA NRACSTPTSFCSVPQVPNTY* -2.17 0.000344 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22732 CPREPRHDTVLLHHFTCRAH 20 SLAY-screened peptide P1082 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCCAGGGAGCCTAGGCATGATACGGTTTTGCTTCACCATTTCACCTGCAGGGCCCATTAA CPREPRHDTVLLHHFTCRAH* -2.17 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22733 PPLASCLAMRGFTITVPQLT 20 SLAY-screened peptide P1083 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCCCTGGCCTCGTGCCTCGCCATGCGTGGCTTTACTATTACTGTTCCGCAGTTGACCTAA PPLASCLAMRGFTITVPQLT* -2.169 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22734 AIFLVLFLFGHKSLFLIYRR 20 SLAY-screened peptide P1084 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCATCTTCCTTGTTTTGTTCCTGTTCGGCCATAAGAGCCTTTTCCTTATTTACCGGCGGTAA AIFLVLFLFGHKSLFLIYRR* -2.169 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22735 FSKEMMKSVHSMAQNALIVS 20 SLAY-screened peptide P1085 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTCGAAGGAGATGATGAAGAGTGTCCATTCCATGGCCCAGAATGCCCTGATTGTTAGCTAA FSKEMMKSVHSMAQNALIVS* -2.168 0.00015 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22736 GLPYDLPDVPNVH 13 SLAY-screened peptide P1086 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCTGCCCTACGATTTGCCTGACGTTCCGAATGTGCATTAGGCGCGCGGTGTTCGCACCTAA GLPYDLPDVPNVH*ARGVRT* -2.167 0.000174 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22737 RDTRCLPTHLLNSSHDPCTA 20 SLAY-screened peptide P1087 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGACACCCGTTGCTTGCCCACCCACCTTTTGAACAGTTCGCACGATCCTTGCACCGCGTAA RDTRCLPTHLLNSSHDPCTA* -2.166 0.000103 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22738 SRASYSNSHGTRHSHCTSSY 20 SLAY-screened peptide P1088 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCGTGCTTCTTACTCTAACTCCCATGGTACCAGGCATTCTCATTGCACCAGTTCCTACTAA SRASYSNSHGTRHSHCTSSY* -2.165 4.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22739 DPLRPNNPTSRFAVYSAIGT 20 SLAY-screened peptide P1089 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCCGCTTCGGCCTAATAATCCGACGTCTCGTTTTGCCGTGTACAGTGCTATTGGCACCTAA DPLRPNNPTSRFAVYSAIGT* -2.164 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22740 QYYVLHRRRLSY 12 SLAY-screened peptide P1090 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTACTATGTACTCCATCGACGTCGATTATCATATTGATGCCTAACTGAGTAAGTCGACCTG QYYVLHRRRLSY*CLTE*VDL -2.163 0.002885 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22741 AN 2 SLAY-screened peptide P1091 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTAATTAGCGCAACGCTTACCCTGAGGTCGTCTACCATATTAACCCCTTTACGCAGCAGTAA AN*RNAYPEVVYHINPFTQQ* -2.162 2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22742 CDRVSTCAAVGLLYFCKRFA 20 SLAY-screened peptide P1092 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGACAGGGTCTCCACCTGCGCGGCCGTCGGGCTTCTCTACTTTTGTAAGCGTTTTGCTTAA CDRVSTCAAVGLLYFCKRFA* -2.162 1.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22743 YRVRGPEKRTHVVLVSC 17 SLAY-screened peptide P1093 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCGTGTGAGGGGTCCTGAGAAGAGGACGCATGTTGTCCTTGTCTCGTGCTAGTATCCCTAA YRVRGPEKRTHVVLVSC*YP* -2.162 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22744 PCTYFDSLTRWFMLDAYPNL 20 SLAY-screened peptide P1094 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGTACGTACTTCGATAGTCTCACTCGTTGGTTCATGTTGGATGCTTACCCTAACTTGTAA PCTYFDSLTRWFMLDAYPNL* -2.162 0.003911 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22745 RY 2 SLAY-screened peptide P1095 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTACTAGGGCCCGATTTATTTCTGCGAGTGCCGTTCTAATTTCTGTCACTATTCGTCTTAA RY*GPIYFCECRSNFCHYSS* -2.161 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22746 PRSSDNQCQILHCNSLYNNY 20 SLAY-screened peptide P1096 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCGCTCGTCCGACAATCAGTGCCAGATTCTTCACTGTAATAGCCTGTACAATAATTATTAA PRSSDNQCQILHCNSLYNNY* -2.161 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22747 FPYNPRCYWPSYHPVDNYSV 20 SLAY-screened peptide P1097 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCCTTACAATCCTAGGTGTTATTGGCCCTCCTACCACCCTGTTGATAATTATAGCGTTTAA FPYNPRCYWPSYHPVDNYSV* -2.16 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22748 TPPSPSNTPKYVQVTMAYHL 20 SLAY-screened peptide P1098 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCCGCCCAGCCCGAGTAATACTCCCAAGTACGTGCAGGTTACTATGGCCTACCATCTCTAA TPPSPSNTPKYVQVTMAYHL* -2.159 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22749 WPSNLVRRFFQPTMLHHSAV 20 SLAY-screened peptide P1099 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCCCTCCAACTTGGTGCGCAGGTTCTTTCAGCCCACTATGCTCCATCACTCGGCCGTGTAA WPSNLVRRFFQPTMLHHSAV* -2.159 3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22750 AHEQVVNSNCPNNDLPCP 18 SLAY-screened peptide P1100 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCATGAGCAGGTGGTCAATTCCAATTGCCCCAATAATGACCTTCCGTGCCCTTAGGTCTAA AHEQVVNSNCPNNDLPCP*V* -2.159 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22751 ASSSHPILAFNTTLDRINDL 20 SLAY-screened peptide P1101 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAGCAGTAGTCACCCTATCCTGGCTTTTAATACTACCCTGGATCGGATCAATGACCTCTAA ASSSHPILAFNTTLDRINDL* -2.158 4.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22752 LPPVPLLFLRHFRVCYSHLP 20 SLAY-screened peptide P1102 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCCCCCGTGCCGTTGCTCTTCCTTCGCCACTTCCGCGTCTGTTACTCTCATCTTCCCTAA LPPVPLLFLRHFRVCYSHLP* -2.156 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22753 PGSLNAAGGLLSVTTYPYDY 20 SLAY-screened peptide P1103 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGGGTCGCTCAATGCCGCCGGTGGGCTCCTCTCTGTCACTACTTATCCGTACGATTATTAA PGSLNAAGGLLSVTTYPYDY* -2.155 0.000391 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22754 PLGTPPRFHLRISTLIRSTG 20 SLAY-screened peptide P1104 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTGGGACCCCTCCCCGGTTTCATCTGCGGATCTCCACGCTCATCAGGTCCACTGGCTAA PLGTPPRFHLRISTLIRSTG* -2.154 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22755 PSPWGGSSLAL 11 SLAY-screened peptide P1105 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTCCCCCTGGGGCGGGAGCTCCCTCGCTCTGTAGCCGTAGAGTCATCAGACTGCTTTGTAA PSPWGGSSLAL*P*SHQTAL* -2.153 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22756 LPHTE 5 SLAY-screened peptide P1106 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCCCATACCGAGTAGAGTGCTCGTTTGACGCGGGATGTGTTTGCCTACGGTATTGCCTAA LPHTE*SARLTRDVFAYGIA* -2.153 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22757 ERHPFHVHVFRFWAMNSRLR 20 SLAY-screened peptide P1107 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGAGGCACCCTTTTCATGTTCACGTTTTCCGTTTCTGGGCTATGAATAGCAGGCTCCGTTAA ERHPFHVHVFRFWAMNSRLR* -2.153 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22758 CLVLSTCCPNEHRSY 15 SLAY-screened peptide P1108 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTCGTTCTGAGCACCTGTTGCCCCAACGAGCACCGTTCTTATTAGCAGTTGAACCTGTAA CLVLSTCCPNEHRSY*QLNL* -2.152 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22759 FARAVVSEHAHYHCYQNIAP 20 SLAY-screened peptide P1109 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGCTCGCGCCGTCGTTTCGGAGCATGCCCATTATCACTGTTATCAGAATATTGCGCCCTAA FARAVVSEHAHYHCYQNIAP* -2.152 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22760 PWNTNLH 7 SLAY-screened peptide P1110 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGGAATACTAATCTTCATTAGCATTGTACCTGCTCTAGCAATATCCCTCAGGATCACTAA PWNTNLH*HCTCSSNIPQDH* -2.152 0.002013 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22761 HPDYLLAGNRTKSPKDNAQR 20 SLAY-screened peptide P1111 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCTGATTACCTCTTGGCGGGTAACCGTACTAAGTCCCCCAAGGACAACGCCCAGCGTTAA HPDYLLAGNRTKSPKDNAQR* -2.152 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22762 IRDNIPTACSHCTNGTDQVT 20 SLAY-screened peptide P1112 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCGCGATAATATCCCGACCGCGTGTTCGCATTGCACTAATGGTACGGATCAGGTCACCTAA IRDNIPTACSHCTNGTDQVT* -2.151 0.000784 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22763 PNGFNTPSFTIRSVYRLVFA 20 SLAY-screened peptide P1113 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAACGGGTTCAATACCCCCAGTTTTACTATTCGTTCCGTTTATAGGCTGGTCTTTGCTTAA PNGFNTPSFTIRSVYRLVFA* -2.151 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22764 LLHVPTPLLMQKLLFRHLYH 20 SLAY-screened peptide P1114 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCTCCATGTGCCCACCCCCCTTCTTATGCAGAAGCTCTTGTTTAGGCATCTCTATCACTAA LLHVPTPLLMQKLLFRHLYH* -2.151 0.000555 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22765 DLLDAGSHH 9 SLAY-screened peptide P1115 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTTGCTCGACGCTGGGTCCCATCACTAGCACCTTGGGTACAGCTTGACTTGGTCTTTTTAA DLLDAGSHH*HLGYSLTWSF* -2.15 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22766 AQSFAFLFLRKMALEPRQLI 20 SLAY-screened peptide P1116 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCAGTCTTTTGCGTTTCTGTTTCTCCGCAAGATGGCTCTGGAGCCGCGGCAGCTTATCTAA AQSFAFLFLRKMALEPRQLI* -2.15 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22767 ATIYKWPLDHYTHANSSNYR 20 SLAY-screened peptide P1117 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTACCATTTATAAGTGGCCTTTGGACCATTACACGCATGCTAATTCTTCGAATTATAGGTAA ATIYKWPLDHYTHANSSNYR* -2.149 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22768 CGFGLPSFRCTDHFRYVIYD 20 SLAY-screened peptide P1118 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGGCTTTGGGCTCCCCTCCTTTAGGTGCACGGACCACTTCCGCTATGTGATTTACGATTAA CGFGLPSFRCTDHFRYVIYD* -2.149 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22769 RTPPDSPFAILIFWRRSRCVN 21 SLAY-screened peptide P1119 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGACCCCTCCGGATTCTCCGTTTGCCATACTGATATTCTGGCGTCGCAGCAGATGCGTTAAC RTPPDSPFAILIFWRRSRCVN -2.149 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22770 YSDIVKFNMLKDPQSDSVAS 20 SLAY-screened peptide P1120 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTCCGATATTGTGAAGTTTAATATGCTTAAGGACCCCCAGAGCGACTCCGTCGCCTCCTAA YSDIVKFNMLKDPQSDSVAS* -2.148 0.000506 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22771 NTLDYNYPLIHLRDYVKTLC 20 SLAY-screened peptide P1121 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACACCCTGGATTATAATTACCCGTTGATTCATCTGCGCGATTACGTTAAGACGCTTTGTTAA NTLDYNYPLIHLRDYVKTLC* -2.148 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22772 NLLSQVYAPRPLGLLSRLKS 20 SLAY-screened peptide P1122 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCTGCTTTCCCAGGTTTATGCTCCTCGGCCTCTTGGCTTGCTCAGCCGTCTTAAGTCTTAA NLLSQVYAPRPLGLLSRLKS* -2.147 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22773 MYSNSLRTCAYSDTHSASVD 20 SLAY-screened peptide P1123 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTACTCTAATTCTCTCCGGACGTGTGCCTATAGCGATACTCACAGTGCCAGCGTGGACTAA MYSNSLRTCAYSDTHSASVD* -2.147 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22774 PAPYRTYAHLSGRCLNQYTR 20 SLAY-screened peptide P1124 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCCCCCTATCGTACCTACGCTCACTTGTCGGGTCGTTGTTTGAACCAGTATACTCGCTAA PAPYRTYAHLSGRCLNQYTR* -2.147 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22775 CDYTLPTTAPTRPPSAPTWR 20 SLAY-screened peptide P1125 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGACTATACGCTTCCCACTACTGCTCCGACCCGTCCGCCCTCGGCGCCTACTTGGCGCTAA CDYTLPTTAPTRPPSAPTWR* -2.146 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22776 ASSTRHGSEPHGSIHSPYCY 20 SLAY-screened peptide P1126 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTAGCTCTACCAGGCATGGGTCCGAGCCCCACGGGAGTATCCATAGTCCTTATTGTTATTAA ASSTRHGSEPHGSIHSPYCY* -2.146 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22777 GEGELIRVSKHCHLDAIMSG 20 SLAY-screened peptide P1127 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGGAGGGGGAGCTCATCAGGGTCTCTAAGCACTGTCATCTTGATGCTATCATGTCCGGCTAA GEGELIRVSKHCHLDAIMSG* -2.146 0.00014 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22778 HRRCASVSLAPRNALPTDHL 20 SLAY-screened peptide P1128 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGCCGCTGCGCCTCGGTCTCGCTCGCCCCGCGGAACGCCCTGCCGACTGATCACTTGTAA HRRCASVSLAPRNALPTDHL* -2.146 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22779 SIKFRSTRYLVRGVSTLFDF 20 SLAY-screened peptide P1129 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCATTAAGTTTCGGAGTACCCGTTATCTCGTTCGCGGTGTCAGCACCTTGTTTGACTTTTAA SIKFRSTRYLVRGVSTLFDF* -2.144 2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22780 TLIYITWTSSPRLMNIITAN 20 SLAY-screened peptide P1130 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCTGATCTATATTACTTGGACCTCGTCCCCTCGTTTGATGAACATTATTACCGCGAATTAA TLIYITWTSSPRLMNIITAN* -2.144 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22781 GFAHYLL 7 SLAY-screened peptide P1131 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTTCGCGCACTACCTCCTCTAGTGTCTTCACCACGACTATGGCGACGCGAATAACGATTAA GFAHYLL*CLHHDYGDANND* -2.143 0.00019 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22782 LGPAFVGRHPRCPQFFDWSR 20 SLAY-screened peptide P1132 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGGGCCCGCCTTTGTGGGTCGTCATCCGCGTTGTCCGCAGTTTTTTGATTGGTCTCGCTAA LGPAFVGRHPRCPQFFDWSR* -2.143 0.000636 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22783 PPTACFAEPLSNYSFPYVLL 20 SLAY-screened peptide P1133 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCGACTGCCTGCTTCGCGGAGCCGCTTTCCAACTATTCCTTCCCTTATGTGCTGCTCTAA PPTACFAEPLSNYSFPYVLL* -2.143 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22784 PRTVFVI 7 SLAY-screened peptide P1134 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGTACTGTCTTTGTCATCTAGCTCATTGTGCCGGATCATAATATTGATGGTAATACGTAA PRTVFVI*LIVPDHNIDGNT* -2.142 0.003074 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22785 GAYAFHHWLFSYLVYMRRMN 20 SLAY-screened peptide P1135 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGGCGTATGCTTTTCACCACTGGCTCTTTAGCTACTTGGTCTATATGAGGAGGATGAACTAA GAYAFHHWLFSYLVYMRRMN* -2.142 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22786 HFVPVYSNLFFLTFPRVIDT 20 SLAY-screened peptide P1136 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTTTGTTCCGGTTTATTCCAACCTTTTCTTCCTTACTTTCCCTAGGGTTATTGATACCTAA HFVPVYSNLFFLTFPRVIDT* -2.14 2.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22787 PSAELCMPNAFPGLPNGSSP 20 SLAY-screened peptide P1137 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCTGCGGAGCTTTGCATGCCCAATGCTTTCCCTGGTCTCCCTAACGGCTCTAGTCCGTAA PSAELCMPNAFPGLPNGSSP* -2.139 0.001087 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22788 STLVLIRTCHSAISTYYCPS 20 SLAY-screened peptide P1138 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTACCCTGGTCCTTATTAGGACCTGCCATTCGGCTATCTCTACTTACTATTGTCCCTCCTAA STLVLIRTCHSAISTYYCPS* -2.138 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22789 THECTLTLVQHDI 13 SLAY-screened peptide P1139 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCACGAGTGCACCCTCACGCTCGTGCAGCACGATATCTAGGCGAGTCAGCTGATCACGTAA THECTLTLVQHDI*ASQLIT* -2.138 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22790 WLVPSRRLSAYCVLIFSRSD 20 SLAY-screened peptide P1140 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCTTGTCCCGAGTCGCCGGCTGTCGGCGTATTGCGTTCTTATTTTTTCTCGTTCTGATTAA WLVPSRRLSAYCVLIFSRSD* -2.137 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22791 TCAVASRIRSSYKYCTNTTH 20 SLAY-screened peptide P1141 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTGTGCGGTTGCGTCGCGTATTCGCTCTTCGTATAAGTACTGTACGAACACCACCCATTAA TCAVASRIRSSYKYCTNTTH* -2.137 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22792 RSYTEETYLSYFVYTNRCDRY 21 SLAY-screened peptide P1142 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTCCTACACGGAGGAGACTTACCTCTCTTACTTTGTTTATACGAACCGGTGCGATCGGTAC RSYTEETYLSYFVYTNRCDRY -2.137 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22793 FTSQALSADISDDTCWAHAQ 20 SLAY-screened peptide P1143 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTACTTCTCAGGCGCTTTCCGCCGACATCAGTGACGATACCTGTTGGGCGCATGCCCAGTAA FTSQALSADISDDTCWAHAQ* -2.137 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22794 TPCNFSHQSCHGLSTGLLTD 20 SLAY-screened peptide P1144 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCCGTGTAACTTCAGCCACCAGTCTTGCCACGGCCTTTCTACTGGCCTGCTTACTGATTAA TPCNFSHQSCHGLSTGLLTD* -2.136 0.001118 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22795 ASPHFCPGSRHSAIPPYMLL 20 SLAY-screened peptide P1145 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCTCCCCTCATTTCTGTCCTGGTTCGAGGCACTCTGCTATTCCGCCCTACATGTTGCTTTAA ASPHFCPGSRHSAIPPYMLL* -2.136 1.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22796 SGWFS 5 SLAY-screened peptide P1146 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGGCTGGTTTAGTTAGCAGCCCCGTGGCGCGTCGCTCAACGAGGCGAATTCTCTTACTTAA SGWFS*QPRGASLNEANSLT* -2.136 0.000744 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22797 TSSPLSLCSSSQALATPSAN 20 SLAY-screened peptide P1147 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTCTTCTCCTTTGTCGCTGTGTTCCAGTAGTCAGGCCTTGGCGACTCCTTCGGCGAACTAA TSSPLSLCSSSQALATPSAN* -2.136 9.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22798 CI 2 SLAY-screened peptide P1148 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTATCTAGCCTCATCTTGAGCGCCAGCATAAGTGCTTCTCCCGTCATATTATGATGCATTAA CI*PHLERQHKCFSRHIMMH* -2.135 0.000173 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22799 WPPCGSHCLF 10 SLAY-screened peptide P1149 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCCCCCTTGTGGCAGTCACTGTTTGTTCTAGAGTTATTCTGATAACGATCCTGACGCGTAA WPPCGSHCLF*SYSDNDPDA* -2.135 0.000535 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22800 RVHKLLPLHSGLSSDSM 17 SLAY-screened peptide P1150 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGTCCATAAGCTGTTGCCGCTCCACAGTGGCCTGTCCAGTGACTCCATGTAGCAGTTCTAA RVHKLLPLHSGLSSDSM*QF* -2.135 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22801 LAAPNQTTEGRDIPNWAHPT 20 SLAY-screened peptide P1151 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGCCGCCCCGAACCAGACTACGGAGGGCCGTGATATCCCGAACTGGGCTCATCCCACTTAA LAAPNQTTEGRDIPNWAHPT* -2.135 0.00384 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22802 AALGSWNSTTYSPLVTPMNR 20 SLAY-screened peptide P1152 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGCTCTTGGTTCTTGGAACTCTACGACGTATTCTCCTCTGGTTACTCCTATGAATCGCTAA AALGSWNSTTYSPLVTPMNR* -2.134 0.00026 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22803 LCSPLYDTF 9 SLAY-screened peptide P1153 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTGTAGTCCTCTTTATGACACGTTTTAGGCCATTTTTTAGGCGTTTAACACCGCCGATTAA LCSPLYDTF*AIF*AFNTAD* -2.133 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22804 DNSFGDRCGHYSYHVCISSA 20 SLAY-screened peptide P1154 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATAATTCGTTTGGCGACAGGTGCGGCCATTATAGCTATCATGTGTGTATCTCTTCGGCGTAA DNSFGDRCGHYSYHVCISSA* -2.132 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22805 CLYSHIGVPPSLPTDFYYSR 20 SLAY-screened peptide P1155 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTTGTATTCTCATATTGGCGTGCCGCCCTCGCTTCCGACGGATTTCTACTATAGCAGGTAA CLYSHIGVPPSLPTDFYYSR* -2.132 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22806 LIFYFLRVWPDLPYYCTDSD 20 SLAY-screened peptide P1156 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGATTTTCTACTTTCTGAGGGTCTGGCCTGACCTGCCCTATTACTGTACCGACTCCGATTAA LIFYFLRVWPDLPYYCTDSD* -2.131 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22807 HGIPYYRTVSISDTLAT 17 SLAY-screened peptide P1157 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGGTATCCCTTATTATCGGACTGTTAGCATTAGCGACACCTTGGCGACTTAGTGCTAAGTA HGIPYYRTVSISDTLAT*C*V -2.131 0.002641 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22808 PQNYL 5 SLAY-screened peptide P1158 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCAGAACTACCTGTAGGCTCCGCTTTCGCGCGCTGCTAACCATCACTCCTACACGTCTTAA PQNYL*APLSRAANHHSYTS* -2.131 0.000297 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22809 TGLIRWYQSCRHLPFRRLY 19 SLAY-screened peptide P1159 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGGCTGATTAGGTGGTACCAGTCGTGCCGGCACTTGCCGTTCAGGCGCTTGTACTAGTAA TGLIRWYQSCRHLPFRRLY** -2.13 0.000575 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22810 HLFILNKTHVDCRAH 15 SLAY-screened peptide P1160 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCTTTTTATTCTTAATAAGACTCACGTCGATTGCAGGGCTCATTAGACTATGCACTGTTAA HLFILNKTHVDCRAH*TMHC* -2.129 3.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22811 RVIRRSGGTI 10 SLAY-screened peptide P1161 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGTCATCCGGCGATCCGGAGGAACAATATGAATAAGTCTCATATTATCCAGGGCCTTTAAC RVIRRSGGTI*ISLILSRAFN -2.128 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22812 PELGPPPTIGPRF 13 SLAY-screened peptide P1162 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGAGCTTGGGCCTCCTCCGACTATTGGCCCTCGTTTCTAGAGCCTTTTGGCGCCCGCGTAA PELGPPPTIGPRF*SLLAPA* -2.128 0.000173 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22813 FSTFHMRMYSPEYEPANYHP 20 SLAY-screened peptide P1163 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTCTACCTTTCATATGCGGATGTATTCGCCCGAGTATGAGCCTGCGAACTACCACCCGTAA FSTFHMRMYSPEYEPANYHP* -2.128 0.00104 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22814 YNGVLNGCTRRNIKP 15 SLAY-screened peptide P1164 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACAACGGCGTCCTGAATGGTTGCACCCGTAGGAACATCAAGCCTTAGAAGGCGGCCGCTTAA YNGVLNGCTRRNIKP*KAAA* -2.128 0.003781 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22815 LYLVAGAILRIYHCRLDGSY 20 SLAY-screened peptide P1165 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTATCTCGTGGCGGGTGCTATCCTTCGTATTTATCATTGCAGGCTGGACGGTAGTTATTAA LYLVAGAILRIYHCRLDGSY* -2.127 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22816 FPNPMDLSSPWYVLHGQKVP 20 SLAY-screened peptide P1166 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCCCAATCCTATGGATTTGTCCTCCCCTTGGTATGTCCTCCACGGGCAGAAGGTGCCTTAA FPNPMDLSSPWYVLHGQKVP* -2.127 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22817 RLLLLLLLRSLYDPKARKPR 20 SLAY-screened peptide P1167 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTTGCTCTTGTTGCTTCTTCTGCGTAGTCTCTATGACCCCAAGGCTCGTAAGCCTCGTTAA RLLLLLLLRSLYDPKARKPR* -2.127 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22818 NCGYRTPAPGTSRPA 15 SLAY-screened peptide P1168 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTGCGGGTATCGCACTCCGGCTCCGGGCACTTCCCGTCCGGCTTAGGCGCGCCATCTGTAA NCGYRTPAPGTSRPA*ARHL* -2.126 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22819 SSTRSARAQDPMALSHTHTG 20 SLAY-screened peptide P1169 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCGACTAGGTCGGCCCGGGCCCAGGATCCTATGGCTTTGAGCCATACCCATACTGGCTAA SSTRSARAQDPMALSHTHTG* -2.126 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22820 LPSSDCYITPDSATFLIINI 20 SLAY-screened peptide P1170 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCTAGCTCTGACTGTTACATTACGCCTGATTCCGCTACTTTTCTGATTATCAATATTTAA LPSSDCYITPDSATFLIINI* -2.125 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22821 DYNAFSEETKVYCIYLIHNT 20 SLAY-screened peptide P1171 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTACAACGCTTTCTCCGAGGAGACGAAGGTGTATTGTATTTACCTTATCCACAATACTTAA DYNAFSEETKVYCIYLIHNT* -2.124 0.000804 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22822 HYDWWNVPCHSRYSPSYVLL 20 SLAY-screened peptide P1172 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTACGACTGGTGGAATGTTCCGTGTCATTCTCGTTATTCCCCGAGTTACGTTCTGCTCTAA HYDWWNVPCHSRYSPSYVLL* -2.124 0.000998 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22823 IDLWSFHINGNLASTAASISN 21 SLAY-screened peptide P1173 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGATCTTTGGAGCTTTCACATTAACGGCAACCTTGCGTCCACCGCCGCATCCATATCTAAC IDLWSFHINGNLASTAASISN -2.123 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22824 NADCTRHSIRNDQC 14 SLAY-screened peptide P1174 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGCGGACTGCACGCGTCATAGCATCCGCAATGACCAGTGCTAGAATGCCTAGGGTATTTAA NADCTRHSIRNDQC*NA*GI* -2.123 3.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22825 AQHVIPCQIPYYLPITYVLR 20 SLAY-screened peptide P1175 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCAGCATGTGATCCCTTGCCAGATCCCGTACTATCTCCCGATTACGTATGTGCTCCGTTAA AQHVIPCQIPYYLPITYVLR* -2.123 6.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22826 STYYTLPNVPICPYSHWHPY 20 SLAY-screened peptide P1176 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCACGTATTATACGCTTCCCAACGTGCCTATCTGTCCGTATTCGCATTGGCATCCCTATTAA STYYTLPNVPICPYSHWHPY* -2.123 0.002088 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22827 LPAGYETRRVYLANSAGGTS 20 SLAY-screened peptide P1177 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCTGCGGGGTACGAGACTCGTCGGGTCTATTTGGCCAATAGTGCTGGTGGGACGTCCTAA LPAGYETRRVYLANSAGGTS* -2.122 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22828 NPRLDLTRSSSQFSVSPGRR 20 SLAY-screened peptide P1178 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCCCCGCCTTGATCTGACCCGGAGCTCTTCGCAGTTTTCGGTTTCCCCTGGTCGTAGGTAA NPRLDLTRSSSQFSVSPGRR* -2.122 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22829 HKGAYRVSSLIITTMKMSQR 20 SLAY-screened peptide P1179 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAAGGGCGCCTACCGTGTTAGTAGTCTCATCATCACTACTATGAAGATGTCGCAGCGCTAA HKGAYRVSSLIITTMKMSQR* -2.121 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22830 SLALPIFTFTTHIAPHVTHL 20 SLAY-screened peptide P1180 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCTCGCCCTCCCGATTTTCACGTTTACTACTCATATTGCGCCTCATGTCACGCATCTTTAA SLALPIFTFTTHIAPHVTHL* -2.12 0.002071 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22831 LQYVPLTRSRHTLDSENTAW 20 SLAY-screened peptide P1181 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCAGTATGTCCCCCTGACGCGCAGTCGGCATACGCTTGATTCCGAGAACACCGCTTGGTAA LQYVPLTRSRHTLDSENTAW* -2.118 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22832 PRRNPVRYVMSKIQVIFLRN 20 SLAY-screened peptide P1182 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGGCGGAACCCCGTGCGGTATGTTATGAGTAAGATTCAGGTCATCTTCCTCCGTAATTAA PRRNPVRYVMSKIQVIFLRN* -2.118 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22833 RAGCIPLPPVLVYPNIHPST 20 SLAY-screened peptide P1183 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCGGGCTGTATCCCCCTGCCCCCTGTCCTGGTTTACCCGAACATTCACCCGAGCACTTAA RAGCIPLPPVLVYPNIHPST* -2.118 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22834 SFHAFATDVDAHSSLPGENT 20 SLAY-screened peptide P1184 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTTTCATGCGTTTGCGACGGACGTGGACGCGCATTCGTCCCTTCCTGGTGAGAACACCTAA SFHAFATDVDAHSSLPGENT* -2.117 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22835 YA 2 SLAY-screened peptide P1185 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGCGTAGGCCCAGCTTACCACCAGTAATGCTGGGCCCATTGGGGACTCTACTTCTTCGTAA YA*AQLTTSNAGPIGDSTSS* -2.117 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22836 NLRVKYSLPCTLVANALHHS 20 SLAY-screened peptide P1186 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCTCCGTGTCAAGTATTCGTTGCCTTGTACTTTGGTCGCCAATGCTCTCCACCATAGTTAA NLRVKYSLPCTLVANALHHS* -2.117 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22837 QRPLREARIKPNHHRGFHNL 20 SLAY-screened peptide P1187 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCGCCCGCTCCGCGAGGCGCGTATTAAGCCGAACCATCATCGGGGTTTTCATAATTTGTAA QRPLREARIKPNHHRGFHNL* -2.117 9.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22838 GEHPSVVTIPNNCYCYRCTD 20 SLAY-screened peptide P1188 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTGAGCATCCTTCTGTGGTTACGATTCCTAATAATTGTTACTGCTATAGGTGTACCGATTAA GEHPSVVTIPNNCYCYRCTD* -2.117 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22839 PPGQHNKAPDLEYTNPFRNL 20 SLAY-screened peptide P1189 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCGGGTCAGCACAATAAGGCCCCCGACCTGGAGTATACCAATCCTTTTCGTAACCTGTAA PPGQHNKAPDLEYTNPFRNL* -2.116 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22840 APLWPHQGLSRFSILFITITN 21 SLAY-screened peptide P1190 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCCTCTTTGGCCTCATCAGGGACTAAGTAGGTTTTCGATACTGTTCATCACCATAACTAAC APLWPHQGLSRFSILFITITN -2.116 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22841 DRCPSYHMFNPTVTYICRLG 20 SLAY-screened peptide P1191 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCGTTGTCCTTCGTATCACATGTTCAACCCGACGGTCACCTACATTTGCCGCCTTGGGTAA DRCPSYHMFNPTVTYICRLG* -2.116 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22842 PCQNPTSCHHSRSLSRQA 18 SLAY-screened peptide P1192 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCCAGAACCCCACGAGTTGTCATCATTCCCGTTCGCTTTCGCGCCAGGCCTAGCAGTAA PCQNPTSCHHSRSLSRQA*Q* -2.116 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22843 GNYIWPILSDHIAN 14 SLAY-screened peptide P1193 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTAATTATATTTGGCCGATCCTTTCTGACCATATCGCTAACTAGTGCCTGCTCATTTCTTAA GNYIWPILSDHIAN*CLLIS* -2.116 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22844 PLKGLLVGHRASNDNSNNGR 20 SLAY-screened peptide P1194 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCAAGGGGTTGCTGGTTGGCCACCGCGCGAGCAATGATAACAGTAATAACGGGAGGTAA PLKGLLVGHRASNDNSNNGR* -2.115 0.000739 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22845 VYVCTASDCFMSYT 14 SLAY-screened peptide P1195 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTACGTTTGCACTGCTTCGGATTGTTTCATGTCCTACACCTAGCCCGCGCAAGGAGGTAAC VYVCTASDCFMSYT*PAQGGN -2.115 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22846 CWSSP 5 SLAY-screened peptide P1196 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTGGTCGAGTCCGTAGATCAAGAACAGTCCCACGTATGCGGTTACCCATGTTCCGGCTTAA CWSSP*IKNSPTYAVTHVPA* -2.115 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22847 RKDIVCYYVSSHTYNALDNF 20 SLAY-screened peptide P1197 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTAAGGATATCGTGTGCTACTACGTTAGTAGTCACACGTACAATGCTCTTGATAATTTTTAA RKDIVCYYVSSHTYNALDNF* -2.114 0.005743 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22848 LYTPNRPPTSLRSRTQTVHV 20 SLAY-screened peptide P1198 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTATACCCCCAACCGCCCTCCGACCAGCTTGCGTTCCCGCACGCAGACCGTGCATGTGTAA LYTPNRPPTSLRSRTQTVHV* -2.114 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22849 LLTTPNSHPHYRQ 13 SLAY-screened peptide P1199 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCTCACGACCCCTAACTCGCACCCGCACTATCGCCAGTAGCCTCTTATCTTCACTAGCTAA LLTTPNSHPHYRQ*PLIFTS* -2.114 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22850 TRLPPSLVSSLAISGTYAYG 20 SLAY-screened peptide P1200 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCGCCTGCCTCCTAGCCTCGTGTCTAGCCTTGCTATTTCGGGGACCTACGCTTACGGCTAA TRLPPSLVSSLAISGTYAYG* -2.113 0.000334 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22851 RHESHTHYTSSQVVYVRWLI 20 SLAY-screened peptide P1201 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCACGAGAGCCATACCCATTATACTTCTTCCCAGGTCGTGTATGTGAGGTGGCTTATCTAA RHESHTHYTSSQVVYVRWLI* -2.113 0.003611 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22852 RYNLLRLGPSRDKSIGIDPS 20 SLAY-screened peptide P1202 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTATAACCTGCTCAGGCTCGGGCCTTCTCGTGATAAGTCTATTGGTATTGATCCCAGTTAA RYNLLRLGPSRDKSIGIDPS* -2.113 0.001093 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22853 GGFHNRICKNY 11 SLAY-screened peptide P1203 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGGCTTCCATAATCGCATTTGTAAGAATTATTAGCGGTGTCGCATTTACTTTGACTACTAA GGFHNRICKNY*RCRIYFDY* -2.113 0.000141 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22854 CPYLYDLAYTTSLTLHLTPT 20 SLAY-screened peptide P1204 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCCTACCTGTATGATTTGGCCTACACCACTTCGCTCACGCTCCACCTTACTCCTACGTAA CPYLYDLAYTTSLTLHLTPT* -2.111 8.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22855 PSNLSPTANSLSSNCPITNS 20 SLAY-screened peptide P1205 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTAACCTTAGTCCTACCGCTAATTCCTTGTCTAGTAATTGCCCCATTACCAACTCTTAA PSNLSPTANSLSSNCPITNS* -2.111 0.000186 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22856 TNPSDCISLVSTESRVFLLH 20 SLAY-screened peptide P1206 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAACCCTTCGGATTGTATTAGCCTGGTGTCGACCGAGTCGCGGGTTTTCCTTCTTCATTAA TNPSDCISLVSTESRVFLLH* -2.11 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22857 RCRFFCVTRVIYNAAGIKAL 20 SLAY-screened peptide P1207 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTGTAGGTTCTTTTGCGTGACGAGGGTCATCTACAATGCTGCCGGCATTAAGGCTCTGTAA RCRFFCVTRVIYNAAGIKAL* -2.11 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22858 YLPNELASSSNSNYTGFYCA 20 SLAY-screened peptide P1208 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCTGCCTAATGAGCTGGCCTCTAGTTCCAACAGCAATTATACCGGTTTTTATTGTGCCTAA YLPNELASSSNSNYTGFYCA* -2.109 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22859 VTFLRSGRPYMCMSS 15 SLAY-screened peptide P1209 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCACGTTCCTGCGTAGTGGTCGCCCTTACATGTGTATGTCGTCCTAGGAGGCGTGGACCTAA VTFLRSGRPYMCMSS*EAWT* -2.109 0.001013 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22860 GSYGLPNGLIPHSSIRIDYL 20 SLAY-screened peptide P1210 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTCCTACGGCCTTCCTAACGGTCTCATTCCGCATTCTTCGATCCGCATTGACTATCTTTAA GSYGLPNGLIPHSSIRIDYL* -2.109 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22861 PPATWVPPNTYPTPLVYIPH 20 SLAY-screened peptide P1211 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCGGCCACTTGGGTCCCTCCTAACACGTACCCCACGCCCCTGGTTTATATTCCTCATTAA PPATWVPPNTYPTPLVYIPH* -2.108 2.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22862 INRNVDKMHNLNLVPFLTCF 20 SLAY-screened peptide P1212 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAACCGTAATGTGGATAAGATGCACAATCTCAACCTGGTGCCCTTCCTTACTTGCTTTTAA INRNVDKMHNLNLVPFLTCF* -2.108 0.000555 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22863 DL 2 SLAY-screened peptide P1213 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCTCTAGCATGACAGTAGCTCCTTTTTCAACACCTATGACACTAATACTGACGCCTACTAA DL*HDSSSFFNTYDTNTDAY* -2.107 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22864 IHRHHHGSNSWTYLCAKSNL 20 SLAY-screened peptide P1214 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCATCGGCATCACCACGGTAGTAATTCGTGGACGTATCTTTGCGCCAAGAGCAACTTGTAA IHRHHHGSNSWTYLCAKSNL* -2.107 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22865 QPTQLMYTHDTDNRSCLGLT 20 SLAY-screened peptide P1215 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCCGACTCAGTTGATGTATACTCATGACACGGATAATCGCAGCTGTCTGGGGCTCACTTAA QPTQLMYTHDTDNRSCLGLT* -2.106 0.000548 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22866 DPLHVRDCRILSHNMSNMTY 20 SLAY-screened peptide P1216 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCCGCTTCATGTGCGTGACTGTCGTATCCTCAGCCACAACATGAGTAATATGACTTATTAA DPLHVRDCRILSHNMSNMTY* -2.106 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22867 HFNCPLPTSRDPFESKESFR 20 SLAY-screened peptide P1217 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTTTAATTGCCCTCTTCCTACGAGCCGTGATCCTTTCGAGTCTAAGGAGAGTTTTCGTTAA HFNCPLPTSRDPFESKESFR* -2.106 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22868 LYTPFGYLLWSYSLASRDRK 20 SLAY-screened peptide P1218 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTACACGCCTTTCGGGTATTTGCTTTGGAGTTACTCGCTGGCTAGTCGCGATCGCAAGTAA LYTPFGYLLWSYSLASRDRK* -2.106 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22869 TSLLRHAPHSHRDPRHANTK 20 SLAY-screened peptide P1219 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTCCCTGCTTCGTCACGCGCCGCACAGTCACCGTGATCCTAGGCACGCTAATACGAAGTAA TSLLRHAPHSHRDPRHANTK* -2.105 0.00015 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22870 NSVLWILSPICIRGPSRRTVA 21 SLAY-screened peptide P1220 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAGCGTGCTCTGGATCTTGTCGCCCATTTGCATTCGAGGTCCTAGTCGGCGCACTGTTGCT NSVLWILSPICIRGPSRRTVA -2.105 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22871 RFRATMRSILIMLIGGLT 18 SLAY-screened peptide P1221 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTTCCGCGCTACTATGAGGTCTATACTGATAATGCTTATAGGAGGTCTTACATGAATTAAC RFRATMRSILIMLIGGLT*IN -2.104 0.003267 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22872 CFQFRSHYHLSRTNMYHYYN 20 SLAY-screened peptide P1222 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTTTCAGTTCCGTTCCCACTACCACCTTTCGCGGACCAATATGTACCACTACTATAATTAA CFQFRSHYHLSRTNMYHYYN* -2.103 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22873 CRQLLLEHYNTILDSIVFDH 20 SLAY-screened peptide P1223 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCGTCAGCTCCTGTTGGAGCACTACAATACTATCCTCGATAGTATCGTCTTTGACCATTAA CRQLLLEHYNTILDSIVFDH* -2.103 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22874 PIYTNCLYSTDSLTNNSRWF 20 SLAY-screened peptide P1224 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATTTATACGAATTGTTTGTACAGTACTGACAGCCTCACCAATAATTCTCGTTGGTTTTAA PIYTNCLYSTDSLTNNSRWF* -2.103 0.000324 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22875 PLDPSYGLIGAPRTVNEWAH 20 SLAY-screened peptide P1225 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTCGATCCTTCTTATGGTCTCATCGGGGCCCCGCGGACCGTCAATGAGTGGGCCCACTAA PLDPSYGLIGAPRTVNEWAH* -2.102 0.005148 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22876 VLTTHYDSATNY 12 SLAY-screened peptide P1226 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTTGACCACTCACTATGATTCTGCTACCAATTACTAGCGCCCCTACAATGACTGCTATTAA VLTTHYDSATNY*RPYNDCY* -2.102 8.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22877 RATYQGSSYQLIMY 14 SLAY-screened peptide P1227 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGCTACCTATCAGGGCAGTTCTTATCAGCTTATCATGTACTAGACCGGGAGTTACAAGTAA RATYQGSSYQLIMY*TGSYK* -2.102 3.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22878 RTTPSLAYRSHRVHSYMGDN 20 SLAY-screened peptide P1228 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACTACGCCGTCCCTCGCCTATCGTTCGCACCGTGTTCACAGCTATATGGGTGATAACTAA RTTPSLAYRSHRVHSYMGDN* -2.1 0.003349 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22879 PGMPSPSFAGQRNMPCSRSG 20 SLAY-screened peptide P1229 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGCATGCCGAGTCCTAGTTTCGCCGGCCAGAGGAATATGCCGTGTTCGCGGTCTGGGTAA PGMPSPSFAGQRNMPCSRSG* -2.1 0.002396 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22880 PNNFSANTIKHFSRFDGYCS 20 SLAY-screened peptide P1230 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAATAATTTCTCTGCCAATACTATCAAGCATTTTTCTCGTTTCGACGGTTATTGTAGCTAA PNNFSANTIKHFSRFDGYCS* -2.099 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22881 VRYNMSTNPSHSSHRVKCII 20 SLAY-screened peptide P1231 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCGTTATAATATGTCTACGAACCCTTCTCATAGTTCCCATCGTGTTAAGTGTATCATCTAA VRYNMSTNPSHSSHRVKCII* -2.099 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22882 YYDFGTRVTPYIVHTCYYTG 20 SLAY-screened peptide P1232 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTATGATTTCGGCACTCGCGTCACGCCTTATATTGTTCACACTTGTTATTACACTGGTTAA YYDFGTRVTPYIVHTCYYTG* -2.098 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22883 RELPWHSPRILFFLHLLSKA 20 SLAY-screened peptide P1233 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGAGCTTCCCTGGCACTCCCCCCGCATCTTGTTTTTTCTTCATTTGCTGTCCAAGGCCTAA RELPWHSPRILFFLHLLSKA* -2.098 0.001361 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22884 TNVSRDYRAHIVSWRLCAQP 20 SLAY-screened peptide P1234 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCAACGTGTCCAGGGACTATCGGGCTCACATTGTGTCGTGGCGTCTGTGCGCTCAGCCGTAA TNVSRDYRAHIVSWRLCAQP* -2.097 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22885 ISFLCFGLRFTAFLCLRRLRN 21 SLAY-screened peptide P1235 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTCTTTTCTGTGTTTTGGCCTACGTTTTACCGCCTTCTTATGTCTCCGTCGACTACGTAAC ISFLCFGLRFTAFLCLRRLRN -2.097 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22886 KIFNRNTNLYCYNYHHVVPP 20 SLAY-screened peptide P1236 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGATCTTCAACAGGAATACCAACCTTTATTGCTATAACTACCACCACGTTGTTCCTCCGTAA KIFNRNTNLYCYNYHHVVPP* -2.096 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22887 HHPFDLVWLLLMGNWHNMEN 20 SLAY-screened peptide P1237 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCACCCGTTTGATCTGGTTTGGCTGCTTCTTATGGGCAACTGGCATAATATGGAGAATTAA HHPFDLVWLLLMGNWHNMEN* -2.096 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22888 YIYRLWRNGIIIITPMAGIT 20 SLAY-screened peptide P1238 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATATCTATCGCCTTTGGCGTAATGGTATTATCATTATTACTCCGATGGCTGGCATCACGTAA YIYRLWRNGIIIITPMAGIT* -2.095 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22889 TTQSVARGTTSQSYFNNATH 20 SLAY-screened peptide P1239 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGACGCAGTCCGTCGCCCGTGGGACTACCTCTCAGTCTTACTTTAATAACGCTACTCACTAA TTQSVARGTTSQSYFNNATH* -2.095 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22890 SPALDGIMFQYQAEPGSRAA 20 SLAY-screened peptide P1240 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCCTGCGCTGGATGGTATTATGTTCCAGTATCAGGCCGAGCCTGGCTCGCGCGCGGCCTAA SPALDGIMFQYQAEPGSRAA* -2.095 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22891 PPIRPYPTFHTKACTTRSLRN 21 SLAY-screened peptide P1241 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTATCCGTCCGTATCCTACTTTTCACACTAAGGCGTGTACTACGAGATCGTTGCGTAAC PPIRPYPTFHTKACTTRSLRN -2.094 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22892 PRSTCTPLQTKANLLTPCQL 20 SLAY-screened peptide P1242 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGTTCTACGTGTACGCCCCTCCAGACTAAGGCTAACCTCCTCACTCCGTGTCAGCTCTAA PRSTCTPLQTKANLLTPCQL* -2.094 0.00022 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22893 FAVLPHPNLNFVSSSVYEAV 20 SLAY-screened peptide P1243 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGCGGTTCTGCCTCATCCTAACCTTAATTTCGTTTCCTCGTCCGTCTACGAGGCCGTCTAA FAVLPHPNLNFVSSSVYEAV* -2.094 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22894 RTACVHIRYELVTRRRGRRA 20 SLAY-screened peptide P1244 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACTGCTTGTGTCCACATCCGTTACGAGTTGGTCACCAGGCGGCGTGGGCGCCGCGCGTAA RTACVHIRYELVTRRRGRRA* -2.094 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22895 RLSCHFVAHIFRPNIFNEHT 20 SLAY-screened peptide P1245 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCTCAGTTGTCATTTCGTTGCGCATATTTTTCGGCCCAATATTTTCAATGAGCACACCTAA RLSCHFVAHIFRPNIFNEHT* -2.093 0.00183 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22896 CAYHWNNAASDTYNVHLYTS 20 SLAY-screened peptide P1246 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGCCTACCACTGGAATAACGCTGCGTCGGACACTTATAACGTGCATCTTTATACGTCCTAA CAYHWNNAASDTYNVHLYTS* -2.093 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22897 HSGPCRYGIH 10 SLAY-screened peptide P1247 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTCTGGTCCTTGTAGGTACGGCATTCATTAGCCCTCTGTGCATAATAGTGGCTACCGTTAA HSGPCRYGIH*PSVHNSGYR* -2.093 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22898 ILSRNSPDAPHTQNLAASPY 20 SLAY-screened peptide P1248 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCTCTCCAGGAACAGTCCCGATGCGCCGCACACCCAGAATCTCGCGGCTTCTCCGTATTAA ILSRNSPDAPHTQNLAASPY* -2.092 6.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22899 FYMCINSLRTATRAP 15 SLAY-screened peptide P1249 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTACATGTGTATTAATTCTCTGCGTACTGCTACTCGGGCCCCGTAGCACCTGGATTCGTAA FYMCINSLRTATRAP*HLDS* -2.091 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22900 GSSPVATISNYHIHYRPGAL 20 SLAY-screened peptide P1250 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTCCTCCCCGGTGGCCACCATCTCCAACTACCACATTCATTACAGGCCGGGCGCGCTCTAA GSSPVATISNYHIHYRPGAL* -2.091 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22901 DPRNYISGEHIGSSNN 16 SLAY-screened peptide P1251 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCCGCGTAACTACATCTCTGGCGAGCATATCGGGTCGAGTAATAATTAGAGTATTTCCTAA DPRNYISGEHIGSSNN*SIS* -2.091 0.000176 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22902 PKRRRTFRPTLVTIRIRRSLN 21 SLAY-screened peptide P1252 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAAACGACGCCGCACATTTAGACCCACCCTCGTAACCATCCGCATACGTCGATCTCTTAAC PKRRRTFRPTLVTIRIRRSLN -2.09 0.001394 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22903 NYANYVSAVQRGSSHTHINS 20 SLAY-screened peptide P1253 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTACGCTAATTATGTTAGCGCGGTTCAGCGCGGTAGCTCGCATACGCATATTAATTCCTAA NYANYVSAVQRGSSHTHINS* -2.09 3.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22904 HTRLPTDGFCTLFKVTLLICN 21 SLAY-screened peptide P1254 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACTCGTCTTCCTACTGATGGCTTTTGCACCCTCTTCAAAGTAACACTGTTGATTTGTAAC HTRLPTDGFCTLFKVTLLICN -2.089 0.001995 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22905 PAFSPCFRSPRENRAHLLPF 20 SLAY-screened peptide P1255 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGCTTTCTCCCCTTGCTTCCGCAGTCCGAGGGAGAATAGGGCTCATCTCCTGCCGTTCTAA PAFSPCFRSPRENRAHLLPF* -2.088 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22906 CRTMQAHNWL 10 SLAY-screened peptide P1256 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGCACGATGCAGGCTCATAATTGGCTTTAGGACGTCTCTTCTTACCACAACTCCTTTTAA CRTMQAHNWL*DVSSYHNSF* -2.087 0.000785 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22907 CQVCNAL 7 SLAY-screened peptide P1257 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCAGGTGTGTAATGCGCTTTAGGCCCCTTTTGAGGTGGTCCTTAACCTTCTGTAGCATTAA CQVCNAL*APFEVVLNLL*H* -2.087 0.000449 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22908 TCPKNPHSRTGVTTSKTKGL 20 SLAY-screened peptide P1258 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGCCCGAAGAACCCTCATTCCAGGACCGGTGTTACGACGTCGAAGACTAAGGGGTTGTAA TCPKNPHSRTGVTTSKTKGL* -2.087 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22909 PADLHDPFPIALRIRRPRRQ 20 SLAY-screened peptide P1259 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCCGATTTGCACGACCCGTTCCCGATTGCTCTCCGGATCCGCCGTCCCCGTCGCCAGTAA PADLHDPFPIALRIRRPRRQ* -2.086 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22910 TFYNEAYHQIIGCNIGNTVL 20 SLAY-screened peptide P1260 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTTTTATAACGAGGCTTACCACCAGATTATTGGTTGTAATATCGGCAATACCGTGCTTTAA TFYNEAYHQIIGCNIGNTVL* -2.085 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22911 CDVPPPFNTPLSYGWYNMGH 20 SLAY-screened peptide P1261 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGACGTGCCTCCCCCCTTTAACACTCCGCTCAGTTATGGTTGGTATAATATGGGCCATTAA CDVPPPFNTPLSYGWYNMGH* -2.084 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22912 HLSHTPRSFHNVHTAHRRVC 20 SLAY-screened peptide P1262 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCTTAGTCACACGCCGCGTTCTTTTCATAATGTCCACACGGCTCATCGTCGTGTTTGCTAA HLSHTPRSFHNVHTAHRRVC* -2.084 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22913 HFCPNLPNKRDAHSSSTSTW 20 SLAY-screened peptide P1263 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTTTTGCCCGAACCTTCCCAATAAGAGGGATGCTCACAGTAGCTCGACTAGCACGTGGTAA HFCPNLPNKRDAHSSSTSTW* -2.084 4.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22914 LGRMGMCVLSTTSFKKDTCV 20 SLAY-screened peptide P1264 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGGTCGTATGGGTATGTGCGTGCTCAGCACCACCAGTTTCAAGAAGGACACGTGTGTGTAA LGRMGMCVLSTTSFKKDTCV* -2.084 3.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22915 SLLSNAHTSIYQNCRQAFSD 20 SLAY-screened peptide P1265 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCTGCTCTCTAACGCCCATACGTCTATCTATCAGAATTGCCGCCAGGCTTTTTCCGACTAA SLLSNAHTSIYQNCRQAFSD* -2.084 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22916 RTPHSYYWSPWDHEVLLAYS 20 SLAY-screened peptide P1266 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACCCCTCATAGTTATTATTGGTCTCCGTGGGACCATGAGGTCCTCCTCGCGTACAGCTAA RTPHSYYWSPWDHEVLLAYS* -2.082 0.000177 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22917 EVYRKCNNQIELLFI 15 SLAY-screened peptide P1267 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGTGTACAGGAAGTGCAACAACCAGATTGAGCTGCTCTTCATCTAGATCGCTATGACTTAA EVYRKCNNQIELLFI*IAMT* -2.081 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22918 WKATTTPARHLDDSMSDPYL 20 SLAY-screened peptide P1268 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGAAGGCTACTACGACTCCTGCCCGTCACCTCGATGACTCCATGAGCGACCCTTATTTGTAA WKATTTPARHLDDSMSDPYL* -2.081 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22919 LSYVGSSSAMSPASLPLFLN 20 SLAY-screened peptide P1269 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCCTATGTGGGAAGTAGTTCTGCAATGTCTCCTGCCTCATTGCCATTGTTTCTTAACTGA LSYVGSSSAMSPASLPLFLN* -2.081 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22920 AEIRSASLSTRNFSAQVEST 20 SLAY-screened peptide P1270 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGAGATCCGGTCTGCTTCGCTCTCGACCCGCAACTTCTCGGCCCAGGTGGAGAGTACGTAA AEIRSASLSTRNFSAQVEST* -2.08 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22921 DVLIHTSDLMAVRIMLNNLY 20 SLAY-screened peptide P1271 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATGTCCTTATCCACACTTCCGATTTGATGGCCGTCCGTATTATGCTGAATAATCTCTATTAA DVLIHTSDLMAVRIMLNNLY* -2.08 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22922 PCYDFLATNISSIDYNYYIL 20 SLAY-screened peptide P1272 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGTTACGATTTTCTTGCGACGAATATCTCGTCGATTGACTATAATTATTACATTTTGTAA PCYDFLATNISSIDYNYYIL* -2.08 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22923 RCISSLI 7 SLAY-screened peptide P1273 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTGTATTTCTTCTCTTATTTAGTCTATTCTGAAGTTGCATGTTGCGTCTTTTATTATTTAA RCISSLI*SILKLHVASFII* -2.078 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22924 IDPLSGDPTSGIFSSADHQI 20 SLAY-screened peptide P1274 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGATCCCCTGTCCGGGGACCCTACTTCCGGCATTTTCTCTAGTGCGGACCATCAGATCTAA IDPLSGDPTSGIFSSADHQI* -2.078 0.000335 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22925 TYRAPGCSVYCHTYTLGYNV 20 SLAY-screened peptide P1275 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTACCGGGCGCCTGGTTGCAGTGTTTACTGTCATACGTATACCCTGGGTTATAATGTCTAA TYRAPGCSVYCHTYTLGYNV* -2.078 0.003052 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22926 FSHNDCFNRDFPLTRYNQAY 20 SLAY-screened peptide P1276 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCAGTCACAATGACTGTTTTAATCGTGATTTTCCGCTCACTAGGTATAACCAGGCCTATTAA FSHNDCFNRDFPLTRYNQAY* -2.076 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22927 KLKSLHVLPTSCFPSMDTDL 20 SLAY-screened peptide P1277 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCTTAAGTCCTTGCACGTTCTCCCGACTTCCTGTTTTCCGTCCATGGATACTGACCTTTAA KLKSLHVLPTSCFPSMDTDL* -2.076 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22928 LFLKGGRHRFRCTFSYEALK 20 SLAY-screened peptide P1278 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTTCCTGAAGGGGGGTCGCCACCGCTTTAGGTGTACCTTCAGTTATGAGGCCTTGAAGTAA LFLKGGRHRFRCTFSYEALK* -2.076 0.002542 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22929 LHLLDPAMYNVISIPTNVDT 20 SLAY-screened peptide P1279 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCATTTGCTTGATCCGGCCATGTATAATGTTATCTCTATTCCCACTAACGTTGATACTTAA LHLLDPAMYNVISIPTNVDT* -2.075 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22930 VYNKEPNRCCTGPPIINTIS 20 SLAY-screened peptide P1280 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTACAACAAGGAGCCGAACAGGTGCTGCACTGGCCCTCCTATTATTAATACTATCTCTTAA VYNKEPNRCCTGPPIINTIS* -2.075 0.002365 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22931 AVYCCGSLLSHITTHLIDYH 20 SLAY-screened peptide P1281 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGTTTATTGCTGCGGCTCTCTTCTGTCCCACATCACTACTCACCTGATCGATTACCATTAA AVYCCGSLLSHITTHLIDYH* -2.074 0.001808 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22932 SGSAHSDDLPRAYLYHGSCL 20 SLAY-screened peptide P1282 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGGCTCCGCTCATTCTGATGATCTTCCTAGGGCTTACCTTTACCATGGTTCCTGCCTCTAA SGSAHSDDLPRAYLYHGSCL* -2.074 0.001758 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22933 NTDCVRFCYLMIHSPRWFSI 20 SLAY-screened peptide P1283 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATACTGACTGCGTGCGTTTTTGCTACCTTATGATTCATTCTCCGCGTTGGTTTAGCATTTAA NTDCVRFCYLMIHSPRWFSI* -2.074 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22934 GVTHVSAKRRSNPARNITT 19 SLAY-screened peptide P1284 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGTCACCCACGTTTCCGCTAAGCGTCGGTCGAATCCTGCTCGTAATATTACGACGTAGTAA GVTHVSAKRRSNPARNITT** -2.074 0.002261 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22935 ITLTLAGRPTAHST 14 SLAY-screened peptide P1285 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTACCCTTACCTTGGCTGGCCGCCCTACTGCTCACAGTACCTAGCACTACACCGCTGGCTAA ITLTLAGRPTAHST*HYTAG* -2.073 0.000685 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22936 GHGHHNGATKVRCLDLTWQVT 21 SLAY-screened peptide P1286 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCACGGCCACCATAATGGTGCCACTAAGGTTAGGTGCTTGGATCTCACTTGGCAAGTAACT GHGHHNGATKVRCLDLTWQVT -2.073 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22937 PCLVYCTDLPAVNLPYISVF 20 SLAY-screened peptide P1287 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCCTGGTGTATTGCACGGATCTGCCCGCTGTCAACCTTCCCTACATTTCCGTGTTTTAA PCLVYCTDLPAVNLPYISVF* -2.073 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22938 SEGNAQPA 8 SLAY-screened peptide P1288 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGAGGGCAACGCCCAGCCTGCTTAGTTTCAGTTCTTCTGCCGTCAGGACCGTTATATTTAA SEGNAQPA*FQFFCRQDRYI* -2.073 0.00333 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22939 YAMTGGAWLYTFPEGMVLRY 20 SLAY-screened peptide P1289 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCGATGACTGGTGGTGCCTGGCTCTATACCTTTCCCGAGGGCATGGTTCTCAGGTACTAA YAMTGGAWLYTFPEGMVLRY* -2.072 0.000107 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22940 CTRCHNAGNYPHTYNSSIQDD 21 SLAY-screened peptide P1290 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCACGAGGTGTCACAATGCCGGCAATTATCCTCATACCTATAACTCTTCCATTCAGGACGAT CTRCHNAGNYPHTYNSSIQDD -2.072 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22941 YLCIDNNTPYMHSIN 15 SLAY-screened peptide P1291 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCTTTGTATTGACAATAATACGCCTTACATGCACTCCATTAATTAGTTCTATTTTGGTTAA YLCIDNNTPYMHSIN*FYFG* -2.072 4.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22942 LSNNRRLHHIAGPRENLVNGN 21 SLAY-screened peptide P1292 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAGTAACAATCGGCGCCTGCACCACATCGCTGGCCCTCGCGAGAACCTTGTTAACGGTAAC LSNNRRLHHIAGPRENLVNGN -2.072 0.002805 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22943 TYHYRAPVRSRPLYAGLDLH 20 SLAY-screened peptide P1293 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTACCATTACCGGGCCCCCGTGAGGTCTAGGCCTCTCTACGCGGGGCTCGATCTGCATTAA TYHYRAPVRSRPLYAGLDLH* -2.072 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22944 PCSMPTRNIPRRINLTALRL 20 SLAY-screened peptide P1294 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTGTTCGATGCCGACTCGCAATATCCCTCGTCGTATTAATTTGACGGCGCTCCGTCTGTAA PCSMPTRNIPRRINLTALRL* -2.072 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22945 SALYYYTLCARRNIRSITLY 20 SLAY-screened peptide P1295 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGCCTTGTATTATTACACGCTCTGTGCTCGCCGCAACATCCGTAGTATTACGCTTTATTAA SALYYYTLCARRNIRSITLY* -2.071 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22946 HRYLLALSRMYNRMSAVSNA 20 SLAY-screened peptide P1296 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGGTATCTGCTTGCCCTGTCTCGTATGTATAATCGTATGTCGGCTGTGAGTAATGCTTAA HRYLLALSRMYNRMSAVSNA* -2.071 0.000197 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22947 TILAPRYNRVIPTFVAWTPL 20 SLAY-screened peptide P1297 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGATCCTGGCCCCCCGTTACAATCGTGTTATCCCCACTTTTGTTGCTTGGACTCCCCTTTAA TILAPRYNRVIPTFVAWTPL* -2.071 8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22948 VVAVAGRNVATVHNAEHNMY 20 SLAY-screened peptide P1298 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGTGGCTGTGGCGGGCCGGAATGTTGCGACCGTCCATAATGCCGAGCACAATATGTATTAA VVAVAGRNVATVHNAEHNMY* -2.07 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22949 VGHPPSTLRRWHPFCCSVIV 20 SLAY-screened peptide P1299 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGGGCATCCCCCTAGCACTCTTCGTCGTTGGCACCCCTTTTGCTGCTCTGTCATTGTCTAA VGHPPSTLRRWHPFCCSVIV* -2.069 7.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22950 TGRGLSSVRFHDSPSVVNYA 20 SLAY-screened peptide P1300 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGTAGGGGCCTTAGTTCTGTTCGTTTTCACGATAGTCCGTCCGTCGTTAATTATGCGTAA TGRGLSSVRFHDSPSVVNYA* -2.069 0.000325 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22951 YSMPRHSNTCFIS 13 SLAY-screened peptide P1301 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACAGTATGCCCCGGCACAGCAATACTTGTTTCATTTCTTAGTCCGGTTAGACTGACCATTAA YSMPRHSNTCFIS*SG*TDH* -2.069 0.004988 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22952 LFHFYCGDLCINSPWWLPGS 20 SLAY-screened peptide P1302 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTTTCATTTTTATTGCGGTGATCTCTGTATTAATAGTCCCTGGTGGCTGCCGGGTAGTTAA LFHFYCGDLCINSPWWLPGS* -2.069 0.004447 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22953 HLICVTIRSRPRRRAGTVSN 20 SLAY-screened peptide P1303 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCTGATCTGCGTCACTATCCGCTCACGGCCTCGCCGACGTGCAGGCACAGTTAGTAACTGA HLICVTIRSRPRRRAGTVSN* -2.067 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22954 AIVSHPTPQADPTNPTREYN 20 SLAY-screened peptide P1304 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGATCGTCAGCCACCCGACGCCTCAGGCTGACCCTACGAACCCTACCAGGGAGTATAATTAA AIVSHPTPQADPTNPTREYN* -2.067 0.000336 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22955 HYLTFSNIPIAFLDTWE 17 SLAY-screened peptide P1305 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTATTTGACTTTCAGTAATATCCCGATCGCTTTTCTCGATACCTGGGAGTAGAAGCAGTAA HYLTFSNIPIAFLDTWE*KQ* -2.067 0.008719 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22956 SVYLDYTRRTRLTFLTTGNQ 20 SLAY-screened peptide P1306 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGTGTACCTCGATTATACCAGGCGTACGAGGCTGACTTTCCTTACTACTGGTAATCAGTAA SVYLDYTRRTRLTFLTTGNQ* -2.067 0.000844 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22957 PFKDYRCHYQALHADNPGSA 20 SLAY-screened peptide P1307 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTTAAGGATTACAGGTGCCATTACCAGGCTCTTCACGCTGATAACCCTGGCAGTGCCTAA PFKDYRCHYQALHADNPGSA* -2.066 0.000289 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22958 AQLRAKYIPTSDGFQCSKYA 20 SLAY-screened peptide P1308 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCAGCTGCGGGCTAAGTACATTCCTACCAGTGATGGCTTCCAGTGCTCGAAGTACGCTTAA AQLRAKYIPTSDGFQCSKYA* -2.065 9.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22959 NKTRPLSNHNLVAVLRLMS 19 SLAY-screened peptide P1309 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAAGACTAGGCCGCTGTCGAACCATAATCTTGTGGCTGTGCTTAGGCTTATGTCTTAGTAA NKTRPLSNHNLVAVLRLMS** -2.065 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22960 DIQRKSYRSCISIYYLTRTI 20 SLAY-screened peptide P1310 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACATTCAGCGCAAGTCTTACCGCAGCTGCATTTCGATTTACTACCTTACTAGGACTATTTAA DIQRKSYRSCISIYYLTRTI* -2.064 0.000166 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22961 YTVSVSTCATAFTVDTSKQD 20 SLAY-screened peptide P1311 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACCGTCTCTGTCTCCACCTGTGCCACCGCTTTTACTGTCGACACGTCGAAGCAGGATTAA YTVSVSTCATAFTVDTSKQD* -2.063 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22962 SPCTLVGIAHSLHDDNPVHH 20 SLAY-screened peptide P1312 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCCTTGCACCCTGGTTGGCATTGCTCATTCTTTGCATGACGACAACCCCGTCCACCATTAA SPCTLVGIAHSLHDDNPVHH* -2.063 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22963 RAPDTLASEPYVPTTGYSHS 20 SLAY-screened peptide P1313 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGCTCCTGATACGCTCGCCTCGGAGCCCTATGTCCCTACGACCGGCTACTCGCATTCCTAA RAPDTLASEPYVPTTGYSHS* -2.062 0.005441 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22964 HYRNLLTVDNHGTYSIKNFC 20 SLAY-screened peptide P1314 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTACCGTAACCTTCTTACTGTTGATAATCATGGCACTTACTCGATTAAGAACTTCTGCTAA HYRNLLTVDNHGTYSIKNFC* -2.061 0.000534 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22965 HADNLTSQPLFHNTNDRHYS 20 SLAY-screened peptide P1315 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGCTGATAATCTCACCTCTCAGCCTCTCTTTCATAATACTAATGATCGTCACTATTCCTAA HADNLTSQPLFHNTNDRHYS* -2.061 2.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22966 IHLFFICIISTALTRRRPRSN 21 SLAY-screened peptide P1316 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCATCTGTTCTTCATATGCATCATTAGCACTGCTCTAACTAGACGCAGGCCTCGTAGTAAC IHLFFICIISTALTRRRPRSN -2.061 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22967 YSLIGIILAAYFRCHHSLSS 20 SLAY-screened peptide P1317 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTCTCTTATTGGGATTATTCTCGCCGCCTACTTTAGGTGCCATCACAGTTTGAGTAGCTAA YSLIGIILAAYFRCHHSLSS* -2.061 0.003834 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22968 HMPHTTWHVH 10 SLAY-screened peptide P1318 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACATGCCGCATACCACTTGGCATGTGCACTAGAGGGCGACGACGAGTCATCCTTCTCTCTAA HMPHTTWHVH*RATTSHPSL* -2.06 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22969 RLPWLWYIVYLKMPLLARIAN 21 SLAY-screened peptide P1319 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCTGCCCTGGCTGTGGTACATTGTCTATCTTAAGATGCCCCTCCTAGCAAGAATTGCTAAC RLPWLWYIVYLKMPLLARIAN -2.059 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22970 IPSLSFTIILS 11 SLAY-screened peptide P1320 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCCTAGCCTAAGTTTCACTATAATTTTAAGTTGATTTGGCGTAAGACCGCTCACTTTTAAC IPSLSFTIILS*FGVRPLTFN -2.059 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22971 NDQHYTALCHYCCVDPGCYT 20 SLAY-screened peptide P1321 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGATCAGCACTACACGGCGCTCTGCCATTATTGTTGTGTTGACCCGGGTTGTTACACTTAA NDQHYTALCHYCCVDPGCYT* -2.059 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22972 PPYTAAPPFVFRVNSALNSH 20 SLAY-screened peptide P1322 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCTACACGGCCGCTCCTCCTTTTGTTTTCCGCGTTAACTCCGCTCTCAATAGCCACTAA PPYTAAPPFVFRVNSALNSH* -2.058 0.000578 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22973 RRCTHDMYLLTSYEPLLRNT 20 SLAY-screened peptide P1323 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAGGTGCACCCACGACATGTATCTCCTGACCAGCTACGAGCCCCTGCTGCGCAACACTTAA RRCTHDMYLLTSYEPLLRNT* -2.058 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22974 CRPSNKYAPCRPSLRHILYF 20 SLAY-screened peptide P1324 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGCCCTTCTAACAAGTACGCTCCTTGCCGCCCTTCGCTTCGCCACATTTTGTACTTTTAA CRPSNKYAPCRPSLRHILYF* -2.058 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22975 DSSAPYLYFLIIFTARSRWS 20 SLAY-screened peptide P1325 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATAGTTCTGCCCCGTACCTCTATTTTCTCATCATCTTCACCGCTCGCTCCCGTTGGTCGTAA DSSAPYLYFLIIFTARSRWS* -2.057 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22976 PVHTRTALPGPCTIRPQVRP 20 SLAY-screened peptide P1326 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGTCCACACTCGTACGGCTTTGCCCGGCCCTTGCACTATCCGCCCGCAGGTCAGGCCGTAA PVHTRTALPGPCTIRPQVRP* -2.057 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22977 ATYLWTHNYHLIVYPTTRPR 20 SLAY-screened peptide P1327 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGACCTACCTTTGGACGCACAATTACCACCTTATTGTGTATCCTACCACGCGCCCTCGCTAA ATYLWTHNYHLIVYPTTRPR* -2.055 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22978 DQLPLDTWLPLLGCHPLIRI 20 SLAY-screened peptide P1328 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCAGCTCCCCCTGGACACTTGGTTGCCTCTTCTCGGGTGTCATCCCCTTATTCGCATTTAA DQLPLDTWLPLLGCHPLIRI* -2.055 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22979 PYHKMSLNAVMSVSELYLTP 20 SLAY-screened peptide P1329 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTATCATAAGATGTCTCTCAATGCTGTTATGAGTGTGAGTGAGTTGTACTTGACTCCCTAA PYHKMSLNAVMSVSELYLTP* -2.054 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22980 CHVPYLHKGTYVPAFTLDEP 20 SLAY-screened peptide P1330 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCACGTTCCGTACCTCCATAAGGGTACCTATGTCCCCGCGTTCACGCTTGATGAGCCCTAA CHVPYLHKGTYVPAFTLDEP* -2.052 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22981 LTPTPTATRHRTFASNNVYK 20 SLAY-screened peptide P1331 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACTCCTACTCCTACCGCCACGCGGCACCGCACTTTTGCTTCGAACAATGTCTACAAGTAA LTPTPTATRHRTFASNNVYK* -2.052 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22982 TLNNT 5 SLAY-screened peptide P1332 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTGAATAACACGTAGCCTATGCAGGAGCTCACTGGCGGCGTCATCAACAACTGCATTTAA TLNNT*PMQELTGGVINNCI* -2.052 0.000205 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22983 NSWKGTRG 8 SLAY-screened peptide P1333 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTCCTGGAAGGGTACCCGAGGCTGATCTTTATTGAGTAGACTTATCCCCCTACCCTTTAAC NSWKGTRG*SLLSRLIPLPFN -2.051 0.006186 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22984 PMGVAPIYGPPLDAANFSLH 20 SLAY-screened peptide P1334 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGATGGGCGTGGCTCCGATTTATGGTCCTCCTCTCGACGCTGCTAACTTCTCTCTGCATTAA PMGVAPIYGPPLDAANFSLH* -2.051 4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22985 SRSQYYFPVDFMALRLLLSLN 21 SLAY-screened peptide P1335 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCGCAGCCAGTACTACTTCCCTGTCGACTTTATGGCGCTCCGTTTGCTACTTTCGCTTAAC SRSQYYFPVDFMALRLLLSLN -2.051 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22986 APIYNHNCVMLCINMAPRHP 20 SLAY-screened peptide P1336 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCCGATTTATAACCACAATTGTGTTATGCTTTGCATCAATATGGCTCCGCGTCATCCCTAA APIYNHNCVMLCINMAPRHP* -2.051 2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22987 LPHNIWHRTGLLTHNWTARS 20 SLAY-screened peptide P1337 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCTCATAATATCTGGCATCGGACCGGCCTGTTGACTCACAATTGGACCGCTCGCTCCTAA LPHNIWHRTGLLTHNWTARS* -2.051 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22988 PHVRDKPSRARIFTSTWHVI 20 SLAY-screened peptide P1338 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCACGTCCGGGACAAGCCCTCCCGGGCTAGGATCTTCACCTCCACTTGGCATGTTATTTAA PHVRDKPSRARIFTSTWHVI* -2.051 0.000673 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22989 LKLATCLYNMHCQ 13 SLAY-screened peptide P1339 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAAGCTCGCGACGTGTCTTTACAATATGCATTGCCAGTAGGGTAATGACATTCATGTTTAA LKLATCLYNMHCQ*GNDIHV* -2.05 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22990 NVREPVHNHDNWIRGPPLFLT 21 SLAY-screened peptide P1340 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGTCCGCGAGCCCGTGCACAACCATGACAACTGGATCCGGGGCCCGCCCTTGTTCCTAACT NVREPVHNHDNWIRGPPLFLT -2.05 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22991 VHNSVLSPLNYDLFTSRKYA 20 SLAY-screened peptide P1341 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCACAATTCCGTGCTGAGCCCCCTCAATTATGACCTTTTTACTTCCAGGAAGTACGCTTAA VHNSVLSPLNYDLFTSRKYA* -2.049 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22992 NAYWTNMILCIIAFWLHTNL 20 SLAY-screened peptide P1342 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGCCTATTGGACTAACATGATCTTGTGTATCATCGCCTTTTGGCTTCACACGAACCTTTAA NAYWTNMILCIIAFWLHTNL* -2.049 0.000529 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22993 IR 2 SLAY-screened peptide P1343 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCGTTAGGACGACGCTGACTACCCCGCGGTGCTTACTTCGACCACTTATACGCGTCCGTAA IR*DDADYPAVLTSTTYTRP* -2.048 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22994 WAYNNPDD 8 SLAY-screened peptide P1344 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGGCGTACAACAACCCGGACGACTAGAGCCCGTTTCATTTTAACCTTTTGCCGTGCCCTTAA WAYNNPDD*SPFHFNLLPCP* -2.048 0.00115 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22995 TFFFLILFIHVNRNTVHPID 20 SLAY-screened peptide P1345 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTTTTTCTTTTTGATCTTGTTTATCCATGTGAATCGTAATACTGTTCATCCCATCGACTAA TFFFLILFIHVNRNTVHPID* -2.047 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22996 LYHARLYRLYLVASVDSVHS 20 SLAY-screened peptide P1346 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTACCACGCCCGCCTCTACCGCCTTTACCTCGTGGCGTCCGTGGACTCGGTTCACTCGTAA LYHARLYRLYLVASVDSVHS* -2.047 0.002145 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22997 RPSAFCLRNSNSIEFDRPTM 20 SLAY-screened peptide P1347 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCCCAGCGCCTTTTGTCTCCGTAACTCTAATTCGATCGAGTTCGATCGTCCTACTATGTAA RPSAFCLRNSNSIEFDRPTM* -2.047 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22998 PDIYHRINR 9 SLAY-screened peptide P1348 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGATATCTATCACAGGATCAACCGCTAGCTGTGGACTCTGGGTAGTATTCGGAGGACTTAA PDIYHRINR*LWTLGSIRRT* -2.046 0.00019 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP22999 RASSHNSFNH 10 SLAY-screened peptide P1349 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGCTTCTAGCCACAATTCCTTCAACCATTAGCTCAGTTTTCATAAGTGTTTCCATAAGTAA RASSHNSFNH*LSFHKCFHK* -2.046 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23000 GKLYQYVPDIICLITRLITW 20 SLAY-screened peptide P1350 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTAAGCTTTATCAGTATGTTCCCGATATTATTTGCCTTATTACCCGTCTGATTACGTGGTAA GKLYQYVPDIICLITRLITW* -2.046 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23001 GMLDYRPSTYTNLGTTHTGGL 21 SLAY-screened peptide P1351 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCATGTTGGACTACCGCCCTTCGACCTACACGAATCTTGGTACGACGCATACTGGTGGGCTG GMLDYRPSTYTNLGTTHTGGL -2.045 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23002 APGRPRPAEPSNNAYSAPM 19 SLAY-screened peptide P1352 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCCTGGTCGTCCCCGCCCGGCTGAGCCCAGCAACAATGCCTACAGTGCCCCTATGTAGTAA APGRPRPAEPSNNAYSAPM** -2.045 0.007005 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23003 LVVNFRYYKEESNLVPPDFD 20 SLAY-screened peptide P1353 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGTCGTTAACTTTAGGTATTATAAGGAGGAGTCCAATCTCGTCCCGCCGGACTTCGATTAA LVVNFRYYKEESNLVPPDFD* -2.045 0.000913 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23004 LPLNPVDSDAVFTSLREPLY 20 SLAY-screened peptide P1354 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGCCGCTCAATCCTGTTGATAGTGATGCTGTTTTTACCTCCCTCCGCGAGCCTCTTTATTAA LPLNPVDSDAVFTSLREPLY* -2.044 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23005 STFVVS 6 SLAY-screened peptide P1355 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCACTTTCGTGGTGTCCTAACGCGCGGCCCTATCCTCCTTCTGTATGCTGCCTCGGGGTAAC STFVVS*RAALSSFCMLPRGN -2.043 0.005663 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23006 DSMPLFLSCSPSYPSKTPNR 20 SLAY-screened peptide P1356 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTCCATGCCCTTGTTTCTTTCGTGCAGTCCCTCTTACCCGTCTAAGACTCCCAACCGTTAA DSMPLFLSCSPSYPSKTPNR* -2.042 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23007 HTKNSVHIKSCLPVSYRHRP 20 SLAY-screened peptide P1357 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACGAAGAATAGCGTCCACATTAAGTCGTGTTTGCCCGTGTCTTACCGGCACAGGCCCTAA HTKNSVHIKSCLPVSYRHRP* -2.041 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23008 LHDVIGFNRIFILPPILNYN 20 SLAY-screened peptide P1358 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCATGACGTTATCGGGTTCAATCGTATTTTTATTCTTCCCCCGATCCTCAATTATAACTAA LHDVIGFNRIFILPPILNYN* -2.041 0.001454 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23009 HAPMQRLHCRASPFLFVLVV 20 SLAY-screened peptide P1359 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGCTCCCATGCAGCGTCTCCATTGTCGCGCGAGTCCCTTTCTTTTTGTGCTGGTTGTGTAA HAPMQRLHCRASPFLFVLVV* -2.041 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23010 SPDSLKPTRHFFLSTFKTVR 20 SLAY-screened peptide P1360 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCCCGATAGTCTTAAGCCTACGCGCCATTTCTTTCTTAGCACTTTTAAGACCGTTCGTTAA SPDSLKPTRHFFLSTFKTVR* -2.04 0.004689 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23011 PPAGMNASVQYTRYRV 16 SLAY-screened peptide P1361 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCGCCGGCATGAATGCTTCTGTTCAGTACACTCGGTATCGCGTTTAGAGTAAGGGGTAA PPAGMNASVQYTRYRV*SKG* -2.04 0.004712 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23012 LRILYTIRINIDLSDYNVSA 20 SLAY-screened peptide P1362 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAGGATCCTTTACACTATCCGTATCAATATTGATCTCTCTGACTATAACGTGTCGGCGTAA LRILYTIRINIDLSDYNVSA* -2.04 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23013 PTRSLGRLLRFTMCRRRRSIN 21 SLAY-screened peptide P1363 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACTCGTTCTTTGGGGCGTTTACTCCGATTCACCATGTGTCGTAGACGCCGAAGCATTAAC PTRSLGRLLRFTMCRRRRSIN -2.039 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23014 FPNYKGRTRSASPYHPYSIY 20 SLAY-screened peptide P1364 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCCCAATTACAAGGGGCGCACGAGGTCCGCCAGTCCTTACCACCCGTATTCTATCTACTAA FPNYKGRTRSASPYHPYSIY* -2.039 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23015 SHTLALHTARLYIDCVHCNR 20 SLAY-screened peptide P1365 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCACACCCTGGCGCTTCACACCGCCCGGCTTTACATTGATTGTGTTCATTGTAATCGGTAA SHTLALHTARLYIDCVHCNR* -2.039 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23016 PFLLSQSSMDQCFRESTDRT 20 SLAY-screened peptide P1366 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTCCTGCTTAGTCAGTCGTCGATGGACCAGTGTTTCAGGGAGAGCACCGACCGTACCTAA PFLLSQSSMDQCFRESTDRT* -2.039 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23017 GTEAFRAYITRYASCILTYL 20 SLAY-screened peptide P1367 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGACCGAGGCCTTCCGTGCGTATATTACTCGTTACGCTAGTTGCATCCTGACCTATCTGTAA GTEAFRAYITRYASCILTYL* -2.039 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23018 FISTRVTNVQAIRGHPMFRA 20 SLAY-screened peptide P1368 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTATCTCTACCCGCGTTACTAATGTTCAGGCTATCCGCGGCCACCCTATGTTTAGGGCGTAA FISTRVTNVQAIRGHPMFRA* -2.039 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23019 SCLALLLCYLKKLTLRAVHR 20 SLAY-screened peptide P1369 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTGCCTCGCGCTTCTCTTGTGCTACCTTAAGAAGTTGACTCTCCGGGCCGTGCACAGGTAA SCLALLLCYLKKLTLRAVHR* -2.038 0.000601 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23020 RSARHNCLSWDLPNSTSLLRN 21 SLAY-screened peptide P1370 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTCTGCCCGTCACAACTGCCTCAGCTGGGACCTGCCCAATTCCACTTCATTACTTCGTAAC RSARHNCLSWDLPNSTSLLRN -2.038 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23021 VHHMPNVTRHSITGFHLNDTN 21 SLAY-screened peptide P1371 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCACCACATGCCTAACGTTACGAGGCATAGCATTACTGGGTTTCACCTTAACGACACGAAC VHHMPNVTRHSITGFHLNDTN -2.038 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23022 SGLALSYMTVCYVNSHYYSY 20 SLAY-screened peptide P1372 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGGTTTGGCCCTCAGCTATATGACGGTGTGTTATGTCAATAGTCACTACTACTCTTACTAA SGLALSYMTVCYVNSHYYSY* -2.038 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23023 HISNGYRIWFLFCYHFYCRT 20 SLAY-screened peptide P1373 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATCTCCAACGGGTATCGCATTTGGTTCCTGTTTTGTTATCACTTTTACTGTCGCACCTAA HISNGYRIWFLFCYHFYCRT* -2.038 0.000356 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23024 CDFRMPTYKYNYDII 15 SLAY-screened peptide P1374 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGATTTCCGCATGCCTACTTACAAGTACAATTATGACATTATTTAGTTCTCGACGGCGTAA CDFRMPTYKYNYDII*FSTA* -2.037 0.000528 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23025 DPTNNTYSTRIQGSGDSPVY 20 SLAY-screened peptide P1375 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCCTACTAACAACACCTATTCGACGCGTATTCAGGGTAGTGGCGACAGTCCGGTTTATTAA DPTNNTYSTRIQGSGDSPVY* -2.036 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23026 IPSRRQATMAPSYTSF 16 SLAY-screened peptide P1376 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCCCTCGCGCCGTCAGGCCACTATGGCCCCGAGCTATACCTCGTTTTAGACCCCGCTCTAA IPSRRQATMAPSYTSF*TPL* -2.036 0.00012 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23027 LYRASTQRTSGDCGFLFLCL 20 SLAY-screened peptide P1377 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTACCGCGCCTCTACGCAGCGTACCTCTGGCGATTGTGGCTTCTTGTTTCTGTGCCTGTAA LYRASTQRTSGDCGFLFLCL* -2.036 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23028 FSANGDATM 9 SLAY-screened peptide P1378 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTCCGCTAATGGGGATGCTACCATGTAGACTTTCGTCTATAATAGTCTTACTAATGAGTAA FSANGDATM*TFVYNSLTNE* -2.036 5.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23029 PSNLLYHRVHLSPDMHCWGT 20 SLAY-screened peptide P1379 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGCAATTTGCTCTACCATCGTGTGCACTTGAGTCCTGATATGCACTGCTGGGGCACGTAA PSNLLYHRVHLSPDMHCWGT* -2.036 0.00205 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23030 HGIDTHIIYPSAFNYTHTEH 20 SLAY-screened peptide P1380 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGGCATTGATACTCACATTATCTATCCTAGTGCCTTCAACTACACGCATACTGAGCATTAA HGIDTHIIYPSAFNYTHTEH* -2.035 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23031 LSPGLATILRFLSLALALRLL 21 SLAY-screened peptide P1381 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAGTCCCGGGCTTGCTACAATCCTAAGATTCCTTTCCCTCGCTCTAGCCTTACGGCTCCTT LSPGLATILRFLSLALALRLL -2.035 0.000762 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23032 TLVSSSNF 8 SLAY-screened peptide P1382 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTTGTGTCGAGTTCTAACTTCTAGAGCGTCTGTGAGTACGCGATCAACGATTCCTCCTAA TLVSSSNF*SVCEYAINDSS* -2.035 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23033 YPRSAVSTFCFNQGRYSFDV 20 SLAY-screened peptide P1383 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCCCCGCTCTGCGGTTTCCACCTTCTGCTTTAATCAGGGTAGGTACTCTTTTGACGTGTAA YPRSAVSTFCFNQGRYSFDV* -2.034 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23034 QATLANTFDLERYDRNKLPD 20 SLAY-screened peptide P1384 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGCGACGCTGGCTAATACGTTTGACCTCGAGCGGTACGACCGTAACAAGCTGCCGGATTAA QATLANTFDLERYDRNKLPD* -2.034 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23035 NTSPPDKRASSHLRRQTACY 20 SLAY-screened peptide P1385 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACACCAGCCCTCCCGACAAGCGCGCGAGTTCTCATCTCCGCCGTCAGACTGCTTGTTACTAA NTSPPDKRASSHLRRQTACY* -2.033 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23036 ISSNCVHH 8 SLAY-screened peptide P1386 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTTCTAGTAACTGCGTTCATCATTAGGCCAATAATGGGATTATCTTCACGAGGACTTATTAA ISSNCVHH*ANNGIIFTRTY* -2.032 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23037 VRTNLPIDSLPSARSYENST 20 SLAY-screened peptide P1387 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCGGACCAACTTGCCTATTGATTCTCTCCCTTCTGCCCGTTCTTACGAGAATTCGACGTAA VRTNLPIDSLPSARSYENST* -2.031 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23038 LPLRKSHHAA 10 SLAY-screened peptide P1388 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCTCTGCGCAAGAGCCATCATGCGGCTTAGTGTAACCTGCACACTTCGAACAATATTTAA LPLRKSHHAA*CNLHTSNNI* -2.029 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23039 RFTHICGNIGYHNFNIITSS 20 SLAY-screened peptide P1389 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTTTACGCATATTTGTGGTAACATTGGCTACCATAATTTCAACATCATCACCAGTTCCTAA RFTHICGNIGYHNFNIITSS* -2.029 9.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23040 DTGYVPRSLRTYRTATPVSA 20 SLAY-screened peptide P1390 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATACGGGTTATGTCCCTCGGAGCTTGCGGACTTACCGTACGGCCACGCCCGTCTCCGCGTAA DTGYVPRSLRTYRTATPVSA* -2.029 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23041 PQALTDIDSGRSIMHDNSFL 20 SLAY-screened peptide P1391 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCAGGCTCTCACTGATATCGACTCTGGTCGCAGCATTATGCACGACAACAGTTTTTTGTAA PQALTDIDSGRSIMHDNSFL* -2.029 6.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23042 SLM 3 SLAY-screened peptide P1392 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCTGATGTAGGCGCCCCACCACCGCCTGAACTTGATTACGCAGGATATTTGCTCCTCCTAA SLM*APHHRLNLITQDICSS* -2.028 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23043 VPWCTTAPCPPSGEP 15 SLAY-screened peptide P1393 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCCCTGGTGCACTACTGCTCCGTGTCCGCCTTCTGGGGAGCCTTAGCACAAGACGTATTAA VPWCTTAPCPPSGEP*HKTY* -2.028 0.000101 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23044 IGVLHAHASN 10 SLAY-screened peptide P1394 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGGTGTTCTCCACGCCCACGCTTCGAATTAGACCCCCGCGGACCCCTCGGACTTCTCCTAA IGVLHAHASN*TPADPSDFS* -2.028 0.005386 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23045 YNSYVVPCSIRKLFACHCFY 20 SLAY-screened peptide P1395 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAATTCCTACGTGGTTCCTTGTAGTATTCGTAAGCTGTTCGCGTGCCATTGCTTTTACTAA YNSYVVPCSIRKLFACHCFY* -2.028 0.002712 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23046 RSCAGAMRRSIGRWMRLRRR 20 SLAY-screened peptide P1396 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTCGTGCGCCGGCGCGATGCGGCGGTCGATCGGCCGCTGGATGAGGTTGCGGCGGCGTTAA RSCAGAMRRSIGRWMRLRRR* -2.028 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23047 YRTAVRVYTVSKTYFKTDDQ 20 SLAY-screened peptide P1397 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCGTACCGCCGTGCGGGTCTATACGGTTTCGAAGACTTATTTCAAGACTGATGATCAGTAA YRTAVRVYTVSKTYFKTDDQ* -2.028 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23048 AALHYANHISHFVHLDG 17 SLAY-screened peptide P1398 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGCCCTTCATTACGCTAATCACATCTCGCATTTTGTTCACCTGGACGGCTAGTACATTTAA AALHYANHISHFVHLDG*YI* -2.027 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23049 SFDYIALPHFIVRNMRL 17 SLAY-screened peptide P1399 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTTTGACTACATTGCCCTCCCGCATTTTATTGTCCGGAATATGCGCCTCTAGAATTTCTAA SFDYIALPHFIVRNMRL*NF* -2.027 0.001036 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23050 EMNSTRTQWHRSSQPTNPCS 20 SLAY-screened peptide P1400 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGATGAATTCCACTCGCACGCAGTGGCACCGTTCGAGCCAGCCTACTAACCCCTGCAGTTAA EMNSTRTQWHRSSQPTNPCS* -2.027 9.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23051 PALYKIFKRLLHPSTTCPSA 20 SLAY-screened peptide P1401 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCCCTCTACAAGATCTTTAAGCGTTTGTTGCATCCTTCTACCACCTGCCCGTCCGCGTAA PALYKIFKRLLHPSTTCPSA* -2.026 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23052 DLFGSAPWGNMYPYDYLSLRR 21 SLAY-screened peptide P1402 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCTGTTCGGTTCGGCGCCCTGGGGCAACATGTACCCCTACGATTACCTATCACTACGACGT DLFGSAPWGNMYPYDYLSLRR -2.026 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23053 LPKSLMQTLPSRTTHDIFIR 20 SLAY-screened peptide P1403 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCTAAGTCCCTCATGCAGACTCTCCCTAGTCGTACGACTCATGACATCTTTATTAGGTAA LPKSLMQTLPSRTTHDIFIR* -2.026 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23054 EPQASHPTF 9 SLAY-screened peptide P1404 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCCCCAGGCCTCGCATCCTACTTTCTAGTATTCTACGTCGTACGGTATCGGGCCTATTTAA EPQASHPTF*YSTSYGIGPI* -2.026 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23055 PSLIASPGEHTSPSAVWEFG 20 SLAY-screened peptide P1405 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAGTCTGATTGCCAGCCCTGGCGAGCATACCTCTCCCTCCGCCGTGTGGGAGTTCGGCTAA PSLIASPGEHTSPSAVWEFG* -2.026 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23056 CYELWINSQVANPSVSIRSS 20 SLAY-screened peptide P1406 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTACGAGCTGTGGATTAACTCTCAGGTCGCGAACCCGAGCGTTTCTATTCGGTCGTCGTAA CYELWINSQVANPSVSIRSS* -2.025 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23057 SRHNYRKRLFSDLSNGAMNP 20 SLAY-screened peptide P1407 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCGCCATAACTATCGGAAGAGGCTCTTTTCTGATCTTAGCAACGGGGCCATGAATCCTTAA SRHNYRKRLFSDLSNGAMNP* -2.025 0.001096 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23058 DIMFTKRSQCPCHTWTYARS 20 SLAY-screened peptide P1408 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATATTATGTTTACGAAGCGTTCGCAGTGCCCGTGTCATACGTGGACGTACGCGAGGTCTTAA DIMFTKRSQCPCHTWTYARS* -2.025 0.000354 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23059 TMKINASSD 9 SLAY-screened peptide P1409 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCATGAAGATTAATGCTAGCAGTGATTAGTCGGGGTTCGCTCCTCAGTTCACTTTTTAGTAA TMKINASSD*SGFAPQFTF** -2.023 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23060 TVLCMED 7 SLAY-screened peptide P1410 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTCCTTTGTATGGAGGATTAGACGCAGCTTTACCTGTCTCCGAATATTGTCTTGAATTAA TVLCMED*TQLYLSPNIVLN* -2.023 0.008676 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23061 ERSHHQTTVVYTMYLLGANT 20 SLAY-screened peptide P1411 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGAGGAGTCATCATCAGACTACCGTTGTTTATACTATGTACCTGCTTGGTGCGAATACGTAA ERSHHQTTVVYTMYLLGANT* -2.023 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23062 VWGEQSIFFITCGSHTNCMG 20 SLAY-screened peptide P1412 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTGGGGTGAGCAGAGTATTTTTTTTATCACGTGTGGCAGCCACACCAATTGCATGGGCTAA VWGEQSIFFITCGSHTNCMG* -2.023 0.001132 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23063 HFMTAACDVMAYKACVNSML 20 SLAY-screened peptide P1413 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTTCATGACTGCCGCTTGCGATGTTATGGCTTACAAGGCGTGCGTTAACAGCATGCTTTAA HFMTAACDVMAYKACVNSML* -2.021 0.003274 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23064 YLVRGASHNPNPHLFMRTLN 20 SLAY-screened peptide P1414 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCTGGTCCGTGGTGCTAGCCACAATCCTAACCCTCACTTGTTCATGCGCACGCTTAACTAA YLVRGASHNPNPHLFMRTLN* -2.02 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23065 PIYVFASAVAAMCWFVTTYS 20 SLAY-screened peptide P1415 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATTTACGTCTTCGCCAGCGCTGTGGCCGCTATGTGTTGGTTTGTTACTACCTACTCTTAA PIYVFASAVAAMCWFVTTYS* -2.019 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23066 FNTNRLPSVSRTNKFPTVVT 20 SLAY-screened peptide P1416 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTAACACGAATAGGCTTCCTTCTGTGTCCCGTACTAATAAGTTTCCTACCGTTGTCACCTAA FNTNRLPSVSRTNKFPTVVT* -2.019 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23067 NADSTVILTVIFSYGDKCGC 20 SLAY-screened peptide P1417 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGCGGACTCTACTGTTATTCTCACTGTTATTTTTTCTTATGGCGACAAGTGTGGTTGTTAA NADSTVILTVIFSYGDKCGC* -2.019 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23068 LITLHLIIGPFCCLSLRRRM 20 SLAY-screened peptide P1418 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTATCACCCTTCACTTGATCATCGGCCCGTTTTGTTGCCTTTCCCTGAGGAGGCGCATGTAA LITLHLIIGPFCCLSLRRRM* -2.019 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23069 RLPNPNNPNRDPQPPARLCP 20 SLAY-screened peptide P1419 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTGCCCAATCCTAACAACCCCAACCGCGATCCTCAGCCGCCTGCGCGTCTCTGTCCGTAA RLPNPNNPNRDPQPPARLCP* -2.018 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23070 IQERKASCPQNHHFRFTDPL 20 SLAY-screened peptide P1420 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCAGGAGCGCAAGGCCTCCTGTCCTCAGAACCATCACTTCCGCTTTACTGACCCTCTCTAA IQERKASCPQNHHFRFTDPL* -2.018 0.014819 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23071 VLLMPSSTTTVSDAPTTGYM 20 SLAY-screened peptide P1421 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCTCCTTATGCCGTCCAGCACTACTACTGTCAGCGACGCGCCGACTACGGGTTACATGTAA VLLMPSSTTTVSDAPTTGYM* -2.017 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23072 LDLYIRVNIIPEYTGPKYTS 20 SLAY-screened peptide P1422 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGATCTCTACATCCGCGTTAATATCATTCCCGAGTATACTGGCCCTAAGTATACTTCTTAA LDLYIRVNIIPEYTGPKYTS* -2.017 0.010385 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23073 SYISPFNHPVFHRVATHYRS 20 SLAY-screened peptide P1423 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTACATTAGCCCGTTTAATCATCCTGTGTTTCACCGTGTCGCTACTCACTATCGCTCCTAA SYISPFNHPVFHRVATHYRS* -2.017 0.001817 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23074 MRLGLILRMAALLALLALRLT 21 SLAY-screened peptide P1424 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCGGTTGGGACTAATTCTTCGCATGGCAGCTCTGCTTGCCCTCCTGGCCTTACGCCTAACT MRLGLILRMAALLALLALRLT -2.017 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23075 RPTAVDAARRQEIYDYFNFH 20 SLAY-screened peptide P1425 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCCGACTGCGGTTGATGCGGCGCGCCGTCAGGAGATCTATGACTATTTCAATTTCCACTAA RPTAVDAARRQEIYDYFNFH* -2.017 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23076 DTLHLGGHRGNHYIHVFFRF 20 SLAY-screened peptide P1426 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATACTCTGCACCTCGGTGGCCACCGTGGGAACCATTACATTCACGTCTTTTTTAGGTTTTAA DTLHLGGHRGNHYIHVFFRF* -2.016 0.001889 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23077 VPSLAVTGTNN 11 SLAY-screened peptide P1427 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCCCTCCTTGGCCGTTACGGGGACCAATAACTAGATTGCGTACCCCCCCGGCTAGCCCTAA VPSLAVTGTNN*IAYPPG*P* -2.016 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23078 GTGPTAANDAMCRPRHTMNA 20 SLAY-screened peptide P1428 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCACGGGCCCGACGGCCGCGAATGATGCCATGTGTCGGCCGCGGCACACTATGAACGCCTAA GTGPTAANDAMCRPRHTMNA* -2.016 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23079 GCDSHNRYNL 10 SLAY-screened peptide P1429 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTGTGACTCTCATAACCGCTATAACCTCTAGTATCTTTACCGTGAGGTTTGGCCCTACTAA GCDSHNRYNL*YLYREVWPY* -2.016 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23080 LIDLSLDLLTLIWAGRTSRRN 21 SLAY-screened peptide P1430 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTATTGACCTGTCTTTGGACCTGCTCACCTTGATTTGGGCAGGAAGGACCTCACGTCGTAAC LIDLSLDLLTLIWAGRTSRRN -2.013 0.007873 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23081 WYNPTAAP 8 SLAY-screened peptide P1431 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGTATAACCCCACTGCCGCTCCCTAGACGGCGACTCCGACTATTTGGCGGCGCATTTACTAA WYNPTAAP*TATPTIWRRIY* -2.013 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23082 SHTFCGQVINLHANSHTCVR 20 SLAY-screened peptide P1432 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCACACCTTTTGCGGTCAGGTCATTAACCTTCACGCTAACTCCCACACCTGTGTCCGGTAA SHTFCGQVINLHANSHTCVR* -2.013 2.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23083 TLGYIHYTHVEDYVRPFCNY 20 SLAY-screened peptide P1433 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTTGGGTTATATTCACTATACTCACGTCGAGGATTATGTCCGGCCTTTTTGTAATTATTAA TLGYIHYTHVEDYVRPFCNY* -2.012 0.000233 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23084 MAQDPRSNHRYDNKPIHYNV 20 SLAY-screened peptide P1434 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGGCCCAGGATCCCAGGTCTAACCATCGCTATGACAATAAGCCTATTCACTATAATGTTTAA MAQDPRSNHRYDNKPIHYNV* -2.012 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23085 RDHDISSIRPSITNVNDDMQ 20 SLAY-screened peptide P1435 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGATCACGATATCTCTTCTATTCGCCCTAGCATCACTAATGTTAATGACGATATGCAGTAA RDHDISSIRPSITNVNDDMQ* -2.011 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23086 LVFYALIPTLSKNCSPLLSL 20 SLAY-screened peptide P1436 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGTTTTCTATGCCCTTATTCCTACCCTCAGTAAGAATTGTTCTCCTCTTCTTAGCCTTTAA LVFYALIPTLSKNCSPLLSL* -2.011 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23087 YRLTPFNQGLNKIIYCRFRP 20 SLAY-screened peptide P1437 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCGCCTCACTCCCTTTAATCAGGGTCTGAATAAGATTATCTACTGTCGTTTCAGGCCCTAA YRLTPFNQGLNKIIYCRFRP* -2.01 0.000596 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23088 SSHRDIPRPHRLLCLFPYIS 20 SLAY-screened peptide P1438 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTCCCATCGCGATATTCCGAGGCCTCACCGTCTCCTGTGTCTGTTTCCTTATATTTCCTAA SSHRDIPRPHRLLCLFPYIS* -2.01 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23089 ALHDAILSDCICTNPGM 17 SLAY-screened peptide P1439 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCTGCATGATGCCATTCTTAGTGATTGTATCTGTACCAATCCTGGCATGTAGAATATTTAA ALHDAILSDCICTNPGM*NI* -2.01 0.000978 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23090 TRCNWSILEME 11 SLAY-screened peptide P1440 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCGTTGCAATTGGAGCATCCTTGAGATGGAGTAGGCGTATGTTCCGAGTCTTCTCCCTTAA TRCNWSILEME*AYVPSLLP* -2.01 3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23091 TPEDAGGPHCAK 12 SLAY-screened peptide P1441 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCGGAGGACGCGGGCGGTCCTCACTGCGCCAAGTAGCCCGAGGAGATTAATCTCTCGTAA TPEDAGGPHCAK*PEEINLS* -2.01 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23092 PPSS 4 SLAY-screened peptide P1442 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCGTCGAGTTAGGGTTTTCATTATCCTTCGGACTCCAATGCTATTAGGATTAATTGCTAA PPSS*GFHYPSDSNAIRINC* -2.009 0.000438 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23093 LPTLDFPTSKGYMSDRP 17 SLAY-screened peptide P1443 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGCCTACTCTTGATTTTCCGACCAGCAAGGGTTATATGTCCGATAGGCCTTAGGATTTCTAA LPTLDFPTSKGYMSDRP*DF* -2.009 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23094 LSLHHYSSLPVNSHWTRRAS 20 SLAY-screened peptide P1444 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAGTCTCCATCACTACTCCAGCTTGCCCGTGAATTCGCATTGGACTCGTCGGGCTTCCTAA LSLHHYSSLPVNSHWTRRAS* -2.009 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23095 TAPFDWSNVNTPCNTNFVYL 20 SLAY-screened peptide P1445 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGCTCCGTTCGATTGGTCTAACGTGAACACCCCTTGCAATACCAATTTTGTTTACCTTTAA TAPFDWSNVNTPCNTNFVYL* -2.008 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23096 LQTRLFVRREQGSIEFIYP 19 SLAY-screened peptide P1446 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCAGACTCGTCTGTTTGTTCGTAGGGAGCAGGGCTCCATTGAGTTCATCTATCCGTAGTAA LQTRLFVRREQGSIEFIYP** -2.008 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23097 MPLHHSTSGRLTHFLPLASK 20 SLAY-screened peptide P1447 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCCGTTGCACCATTCCACTAGCGGTAGGCTCACTCACTTCCTGCCGCTTGCGTCGAAGTAA MPLHHSTSGRLTHFLPLASK* -2.007 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23098 YHPKHTYRTIDTLDLPYHVT 20 SLAY-screened peptide P1448 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCACCCCAAGCATACCTACCGGACCATCGATACTCTGGATCTGCCTTATCATGTCACCTAA YHPKHTYRTIDTLDLPYHVT* -2.007 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23099 ATCTPLSYRL 10 SLAY-screened peptide P1449 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCACCTGTACTCCCCTTTCCTACCGCCTTTAGATCAACTCTTAGCTGCTCACTCTTGGGTAA ATCTPLSYRL*INS*LLTLG* -2.007 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23100 PRHSVSLALASMPNIPAAYW 20 SLAY-screened peptide P1450 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGGCATAGCGTCTCCCTTGCCCTCGCTAGTATGCCCAATATTCCTGCGGCCTACTGGTAA PRHSVSLALASMPNIPAAYW* -2.007 0.002761 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23101 SEHFGLPPPRHILVPILDDT 20 SLAY-screened peptide P1451 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGAGCACTTCGGCCTGCCTCCGCCGAGGCACATTCTTGTCCCTATTCTCGATGACACGTAA SEHFGLPPPRHILVPILDDT* -2.006 0.018247 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23102 YLNNRLFCRWHGDCHI 16 SLAY-screened peptide P1452 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCTTAACAATCGTCTTTTCTGTCGCTGGCATGGTGATTGTCACATCTGACGTTCACTTAAC YLNNRLFCRWHGDCHI*RSLN -2.006 0.00437 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23103 NHFICFRLLLVYKLSRSGRR 20 SLAY-screened peptide P1453 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCATTTTATTTGCTTCCGCCTGCTTTTGGTTTACAAGCTTAGCCGTAGCGGCCGGCGTTAA NHFICFRLLLVYKLSRSGRR* -2.006 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23104 ACYYFFLFFFRSTYNRHSTD 20 SLAY-screened peptide P1454 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTTGTTACTACTTTTTCCTCTTTTTCTTCAGGAGCACTTACAACCGCCATAGTACTGATTAA ACYYFFLFFFRSTYNRHSTD* -2.006 0.00043 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23105 PPKCDHTRLWALYNDPILIL 20 SLAY-screened peptide P1455 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCAAGTGCGATCACACGCGTCTTTGGGCTTTGTATAACGACCCTATCTTGATTCTTTAA PPKCDHTRLWALYNDPILIL* -2.005 0.00055 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23106 FYPIHHTWYTQLRTCYDSAP 20 SLAY-screened peptide P1456 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTATCCTATCCACCACACCTGGTATACTCAGCTTCGCACCTGTTATGATAGTGCCCCCTAA FYPIHHTWYTQLRTCYDSAP* -2.005 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23107 FRMNSFDHYASIIPTTRGTI 20 SLAY-screened peptide P1457 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCGGATGAACAGCTTTGATCACTATGCGTCTATTATCCCGACGACGCGCGGGACGATTTAA FRMNSFDHYASIIPTTRGTI* -2.005 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23108 PRALWHASDLHSG 13 SLAY-screened peptide P1458 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGCGCTCTTTGGCATGCCAGTGATCTTCATTCTGGTTAGACCCCCACTAAGGTTGGCTAA PRALWHASDLHSG*TPTKVG* -2.004 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23109 SPPIRFSCYLPDSTCSDTTS 20 SLAY-screened peptide P1459 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCCTCCCATTCGTTTTTCTTGTTACCTTCCCGATTCCACCTGCAGTGACACCACTTCGTAA SPPIRFSCYLPDSTCSDTTS* -2.004 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23110 SCIFPKSPIQSYASTDLSIS 20 SLAY-screened peptide P1460 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTGCATCTTCCCTAAGAGCCCGATCCAGTCGTATGCTAGCACGGATTTGTCGATTTCCTAA SCIFPKSPIQSYASTDLSIS* -2.004 0.000201 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23111 RLPRCHTMYLRHSLSHMQIR 20 SLAY-screened peptide P1461 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTCCCTCGCTGTCATACGATGTATCTCCGTCATTCCTTGAGTCACATGCAGATTCGCTAA RLPRCHTMYLRHSLSHMQIR* -2.004 0.001553 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23112 NCA 3 SLAY-screened peptide P1462 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTGTGCTTAGTGCTCGTATAATACTATCCATAACAAGACCTAGAATCTGTTGGGTGTGTAA NCA*CSYNTIHNKT*NLLGV* -2.003 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23113 RQHGYCHFLRYSRHRRMHRH 20 SLAY-screened peptide P1463 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCAGCATGGTTATTGTCATTTCCTTCGTTATTCCCGCCATCGTCGTATGCACCGTCATTAA RQHGYCHFLRYSRHRRMHRH* -2.003 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23114 DHHWLTALVNPHGMVSPNRL 20 SLAY-screened peptide P1464 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCACCACTGGCTCACCGCTCTGGTCAATCCGCACGGCATGGTGTCTCCTAATAGGCTCTAA DHHWLTALVNPHGMVSPNRL* -2.002 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23115 RAGAIPHIARALALFWISSS 20 SLAY-screened peptide P1465 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCCGGCGCGATTCCCCATATCGCGCGCGCTCTCGCTTTGTTTTGGATCTCTTCTTCGTAA RAGAIPHIARALALFWISSS* -2.002 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23116 SYIVKSYLHNRSSATCKTYR 20 SLAY-screened peptide P1466 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTATATTGTCAAGTCTTATCTGCATAATCGGAGTTCGGCTACTTGTAAGACTTACAGGTAA SYIVKSYLHNRSSATCKTYR* -2.002 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23117 ALVYSNRPAIHDGEPLITNT 20 SLAY-screened peptide P1467 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCTCGTCTATAGCAATAGGCCTGCGATTCACGACGGGGAGCCGCTGATTACTAATACCTAA ALVYSNRPAIHDGEPLITNT* -2.001 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23118 LSSLVANAITHCFLLAREIR 20 SLAY-screened peptide P1468 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCGAGTCTCGTTGCTAATGCGATTACCCACTGCTTCTTGCTGGCGCGCGAGATTCGTTAA LSSLVANAITHCFLLAREIR* -2.001 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23119 FPRYMFTATGRLDDGRAMSY 20 SLAY-screened peptide P1469 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCCCAGGTATATGTTTACTGCTACTGGCCGCCTTGATGACGGCCGGGCCATGAGTTATTAA FPRYMFTATGRLDDGRAMSY* -2.0 0.008938 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23120 LVATCSPYHTPTRLEQHNTS 20 SLAY-screened peptide P1470 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGTGGCTACGTGCTCCCCTTATCATACTCCTACGCGTCTGGAGCAGCATAACACTAGTTAA LVATCSPYHTPTRLEQHNTS* -2.0 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23121 ADDTRPRRTITRLLVYAFHT 20 SLAY-screened peptide P1471 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGACGACACTCGCCCCAGGCGTACTATTACCAGGCTTCTTGTCTATGCTTTCCACACGTAA ADDTRPRRTITRLLVYAFHT* -2.0 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23122 FSSHGHQLEAKFIFRPKPNS 20 SLAY-screened peptide P1472 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCAGTTCTCACGGCCATCAGCTTGAGGCCAAGTTCATTTTCAGGCCCAAGCCTAACTCCTAA FSSHGHQLEAKFIFRPKPNS* -1.999 0.000233 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23123 GHYYLCFYLAVTLTIRRIDLT 21 SLAY-screened peptide P1473 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCACTATTATCTCTGTTTCTACCTTGCAGTGACATTGACCATAAGAAGAATAGATCTAACT GHYYLCFYLAVTLTIRRIDLT -1.998 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23124 PLPNLVGTAASNHIFEKSKST 21 SLAY-screened peptide P1474 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTGCCTAACCTGGTTGGGACCGCTGCGAGTAATCATATTTTTGAGAAGAGTAAGTCGACC PLPNLVGTAASNHIFEKSKST -1.997 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23125 HSSIWNRCHIFMHTLDHYPT 20 SLAY-screened peptide P1475 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAGCTCTATCTGGAACCGCTGCCATATTTTTATGCATACGCTTGACCATTACCCCACCTAA HSSIWNRCHIFMHTLDHYPT* -1.997 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23126 INSRRDTGDSRLVFVAYSSP 20 SLAY-screened peptide P1476 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAATTCTCGTAGGGATACTGGCGATTCCCGTTTGGTGTTTGTTGCTTACTCGTCTCCTTAA INSRRDTGDSRLVFVAYSSP* -1.997 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23127 LYGM 4 SLAY-screened peptide P1477 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTACGGGATGTAGTAGCGCCCTCTTGTGGGGTTTATCTATTACTCTAGTCATTAGAAGTAA LYGM**RPLVGFIYYSSH*K* -1.996 0.000222 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23128 ILNWIKGYLHHVLARRNLSS 20 SLAY-screened peptide P1478 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCTGAACTGGATCAAGGGTTATCTTCACCACGTGTTGGCCCGTCGGAATCTTTCGTCTTAA ILNWIKGYLHHVLARRNLSS* -1.996 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23129 NGLDPCYNNCINYLNHIR 18 SLAY-screened peptide P1479 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGGGCTTGATCCCTGCTATAATAACTGTATCAACTATCTCAATCATATCCGGTAGACTTAA NGLDPCYNNCINYLNHIR*T* -1.996 0.000577 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23130 RPCDFLLSNKSVVFQNKNRF 20 SLAY-screened peptide P1480 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCTTGTGATTTTTTGCTTTCTAACAAGTCTGTTGTGTTTCAGAATAAGAACCGGTTTTAA RPCDFLLSNKSVVFQNKNRF* -1.995 0.000375 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23131 RRHPSVLYPCTFQIVYYGLC 20 SLAY-screened peptide P1481 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTAGGCACCCCTCGGTCCTGTACCCCTGCACTTTTCAGATTGTCTACTATGGCCTTTGTTAA RRHPSVLYPCTFQIVYYGLC* -1.995 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23132 DCRSCNYRPTGSWRECGVIP 20 SLAY-screened peptide P1482 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTGTCGCTCCTGTAATTACCGTCCCACGGGGTCCTGGCGCGAGTGTGGTGTCATCCCTTAA DCRSCNYRPTGSWRECGVIP* -1.995 0.000754 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23133 PMYTSPFSAYDRQHNTNCCR 20 SLAY-screened peptide P1483 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATGTACACGAGTCCTTTCTCGGCGTATGACCGTCAGCACAACACTAATTGTTGTCGTTAA PMYTSPFSAYDRQHNTNCCR* -1.995 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23134 LVLGCCLCCRIRFRATINSN 20 SLAY-screened peptide P1484 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGTCCTTGGGTGCTGTTTGTGCTGCCGTATCCGCTTTCGGGCCACTATCAATTCGAACTAA LVLGCCLCCRIRFRATINSN* -1.994 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23135 DNVDNN 6 SLAY-screened peptide P1485 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATAACGTGGATAATAATTAGATTCGCCCTATCGCGACAATGTCAATTACCGTATTCCTTAAC DNVDNN*IRPIATMSITVFLN -1.994 0.005993 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23136 KGYLGRFRPRDTLNDRKTAR 20 SLAY-screened peptide P1486 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGGGTTACCTGGGCCGGTTCCGTCCTAGGGACACTCTCAACGACCGCAAGACTGCGCGGTAA KGYLGRFRPRDTLNDRKTAR* -1.994 0.006092 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23137 SFPSSFLPYVLLDRVERNCS 20 SLAY-screened peptide P1487 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTTCCCTTCCAGCTTCTTGCCCTATGTCCTGCTGGACCGTGTCGAGCGGAATTGCTCCTAA SFPSSFLPYVLLDRVERNCS* -1.993 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23138 PNNYSDSFMAHSHLLDEILN 20 SLAY-screened peptide P1488 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAATAACTATAGCGATAGTTTCATGGCCCACTCTCATTTGCTGGACGAGATCCTCAATTAA PNNYSDSFMAHSHLLDEILN* -1.992 0.002024 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23139 LSQAADRASSCTNGVPLPSQ 20 SLAY-screened peptide P1489 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTCCCAGGCTGCCGATCGGGCTTCCAGTTGTACTAATGGTGTGCCTCTGCCTAGCCAGTAA LSQAADRASSCTNGVPLPSQ* -1.992 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23140 LVDVEFTWLNVSESIILETT 20 SLAY-screened peptide P1490 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGGTCGATGTGGAGTTCACGTGGCTTAATGTTAGTGAGTCCATCATTCTTGAGACCACCTAA LVDVEFTWLNVSESIILETT* -1.992 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23141 LVIQIGLVSWSPRRRSRILRN 21 SLAY-screened peptide P1491 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGTGATTCAGATAGGCCTGGTGTCGTGGTCACCCCGGCGCCGGAGTCGAATACTCCGTAAC LVIQIGLVSWSPRRRSRILRN -1.991 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23142 PRTKFTLNVDHASDHGESPL 20 SLAY-screened peptide P1492 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGGACCAAGTTCACTCTCAATGTCGATCATGCGAGCGACCACGGCGAGAGTCCGTTGTAA PRTKFTLNVDHASDHGESPL* -1.99 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23143 TLVNRHVGKADSGSRPHMYA 20 SLAY-screened peptide P1493 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTGGTGAATCGTCATGTTGGCAAGGCTGATAGCGGTAGTCGCCCTCACATGTACGCGTAA TLVNRHVGKADSGSRPHMYA* -1.989 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23144 RARVPLWDHLSNSPAHNDYT 20 SLAY-screened peptide P1494 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCTCGCGTGCCCCTCTGGGATCATCTTTCTAATTCCCCGGCTCATAACGACTATACTTAA RARVPLWDHLSNSPAHNDYT* -1.988 3.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23145 KCSTMSSSRG 10 SLAY-screened peptide P1495 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGTGCTCGACCATGTCTAGCTCCCGGGGCTAGAGTCTTTTGTACTTGTAGACTAATAGCTAA KCSTMSSSRG*SLLYL*TNS* -1.988 2.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23146 VTTTHQR 7 SLAY-screened peptide P1496 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTACCACTACCCACCAGCGCTAGCTTTCTATCAGTAAGCCGATTTATTCTGCGACGGCTTAA VTTTHQR*LSISKPIYSATA* -1.987 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23147 GANYRRENCSQFSSLQAVNR 20 SLAY-screened peptide P1497 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGCTAACTACCGGAGGGAGAATTGTTCTCAGTTTTCTAGTCTTCAGGCTGTGAATCGGTAA GANYRRENCSQFSSLQAVNR* -1.987 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23148 PYSLAPSFPLVQCYDNSRPL 20 SLAY-screened peptide P1498 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTACAGTCTGGCTCCGAGCTTCCCTTTGGTCCAGTGCTATGACAATAGCAGGCCGTTGTAA PYSLAPSFPLVQCYDNSRPL* -1.987 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23149 SYPTGLAFFAVVAIITRTGRN 21 SLAY-screened peptide P1499 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTACCCTACGGGTCTCGCTTTTTTCGCAGTTGTTGCGATTATAACGAGAACCGGCCGTAAC SYPTGLAFFAVVAIITRTGRN -1.986 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23150 LHHPQKAHSAPAR 13 SLAY-screened peptide P1500 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCATCACCCCCAGAAGGCGCATTCGGCTCCCGCTCGCTAGGCCGCTGCGGCCAGCCACTAA LHHPQKAHSAPAR*AAAASH* -1.985 0.001863 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23151 EPFPFALSQPPLLHGQVKAR 20 SLAY-screened peptide P1501 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCCCTTCCCTTTTGCTCTTAGCCAGCCCCCTCTCCTCCACGGTCAGGTTAAGGCGAGGTAA EPFPFALSQPPLLHGQVKAR* -1.985 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23152 KRNKVN 6 SLAY-screened peptide P1502 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCGGAATAAGGTTAATTAGGGTGCCACGCCCGGCGCTTCGAGTCTGGCCACCGCGCTTTAA KRNKVN*GATPGASSLATAL* -1.985 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23153 GKRLSRTLLAMSLVAV 16 SLAY-screened peptide P1503 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCAAGCGGTTGTCGCGCACGCTCCTAGCCATGAGTTTGGTTGCCGTATGAGCACCCGGTAAC GKRLSRTLLAMSLVAV*APGN -1.985 0.006057 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23154 PILSPDRSRCRLLPVQAYGL 20 SLAY-screened peptide P1504 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGATTCTTTCCCCCGACCGGAGTAGGTGCCGTTTGCTGCCCGTGCAGGCCTATGGGCTGTAA PILSPDRSRCRLLPVQAYGL* -1.985 0.001959 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23155 SYIVYFLPVRRTPARTLGLLN 21 SLAY-screened peptide P1505 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTACATTGTGTATTTCCTCCCCGTGCGCCGTACACCTGCCCGTACACTCGGGTTGCTTAAC SYIVYFLPVRRTPARTLGLLN -1.984 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23156 YSVY 4 SLAY-screened peptide P1506 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTCCGTCTACTAGATTACTGCGTTTAAGTATTTGCCTTACTTTCTGGGGACCCCCCATTAA YSVY*ITAFKYLPYFLGTPH* -1.983 5.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23157 HRTRMLFFISARRNSVFIHN 20 SLAY-screened peptide P1507 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCGCACTAGGATGCTTTTTTTCATCTCTGCGAGGAGGAACTCTGTCTTCATCCACAATTAA HRTRMLFFISARRNSVFIHN* -1.983 0.005663 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23158 HILQASTWLDAVNIGIFTRW 20 SLAY-screened peptide P1508 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATTCTGCAGGCCTCCACTTGGCTGGATGCCGTTAACATCGGTATTTTCACCCGTTGGTAA HILQASTWLDAVNIGIFTRW* -1.982 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23159 PILVDHFLHMHKTDTCHKSR 20 SLAY-screened peptide P1509 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATTCTCGTTGATCATTTTCTCCACATGCACAAGACCGACACCTGCCACAAGTCTCGTTAA PILVDHFLHMHKTDTCHKSR* -1.982 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23160 CIDARKCDCVNDRNVCTVFC 20 SLAY-screened peptide P1510 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCATTGACGCCCGTAAGTGTGATTGTGTTAATGACCGGAACGTTTGCACGGTGTTTTGTTAA CIDARKCDCVNDRNVCTVFC* -1.981 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23161 TAHPALASLLY 11 SLAY-screened peptide P1511 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGCCCATCCTGCGCTTGCGAGCCTCCTTTATTAGGGCCTTTCCTATACGACGGCCTGCTAA TAHPALASLLY*GLSYTTAC* -1.981 0.000375 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23162 MVCLTYTSKPIVSKPLPN 18 SLAY-screened peptide P1512 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGGTTTGTCTGACGTACACCTCGAAGCCTATTGTTTCTAAGCCTCTGCCGAACTAGGACTAA MVCLTYTSKPIVSKPLPN*D* -1.981 0.000366 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23163 SVAQDFFHDFTTAPFCFSII 20 SLAY-screened peptide P1513 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGTTGCTCAGGATTTTTTCCACGACTTCACCACTGCGCCGTTCTGCTTTAGTATTATTTAA SVAQDFFHDFTTAPFCFSII* -1.98 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23164 FQQFYTSGCNTPYYQLIKDY 20 SLAY-screened peptide P1514 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCAGCAGTTCTATACTTCGGGCTGCAACACTCCGTATTATCAGCTGATTAAGGACTACTAA FQQFYTSGCNTPYYQLIKDY* -1.979 0.00016 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23165 NHLHKHPFINGRPGAQGTPP 20 SLAY-screened peptide P1515 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCATCTGCATAAGCACCCGTTCATCAACGGCCGGCCCGGTGCGCAGGGCACGCCGCCTTAA NHLHKHPFINGRPGAQGTPP* -1.979 0.000704 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23166 GAMACQCPPYTTSPCLMSNA 20 SLAY-screened peptide P1516 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGCTATGGCTTGTCAGTGCCCCCCCTATACTACTAGTCCCTGCCTTATGTCGAACGCTTAA GAMACQCPPYTTSPCLMSNA* -1.979 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23167 ANKRGNPCQRPNATG 15 SLAY-screened peptide P1517 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTAATAAGCGGGGTAACCCTTGTCAGCGCCCGAATGCGACTGGTTAGGTTTGTCCCCGTTAA ANKRGNPCQRPNATG*VCPR* -1.979 5.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23168 ARREYPEPTNTCSPAVLLPM 20 SLAY-screened peptide P1518 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCGCCGTGAGTATCCCGAGCCTACGAATACGTGTAGTCCCGCTGTCTTGCTTCCCATGTAA ARREYPEPTNTCSPAVLLPM* -1.978 3.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23169 SSSHTCSTANLILQPSAITT 20 SLAY-screened peptide P1519 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAGCTCTCACACGTGCAGTACTGCTAACTTGATTCTGCAGCCCAGTGCCATTACTACGTAA SSSHTCSTANLILQPSAITT* -1.978 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23170 CHPSSPTFPFIIPVGSDAYT 20 SLAY-screened peptide P1520 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCACCCTAGTTCGCCTACCTTCCCTTTTATTATCCCTGTGGGTAGCGACGCCTATACCTAA CHPSSPTFPFIIPVGSDAYT* -1.977 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23171 NRLFCGDPKDCLWRESSSYE 20 SLAY-screened peptide P1521 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCGCCTGTTTTGTGGGGACCCCAAGGACTGCCTTTGGCGGGAGAGCTCGTCGTATGAGTAA NRLFCGDPKDCLWRESSSYE* -1.977 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23172 IGVHICDLAFSRMAMRELFC 20 SLAY-screened peptide P1522 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGGTGTCCATATTTGCGATCTTGCCTTCAGTCGCATGGCTATGCGTGAGCTTTTCTGTTAA IGVHICDLAFSRMAMRELFC* -1.977 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23173 RWAEPITPQSSFLTAAHPLS 20 SLAY-screened peptide P1523 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTGGGCCGAGCCGATTACGCCCCAGTCCAGTTTTCTCACGGCTGCCCACCCGCTTTCCTAA RWAEPITPQSSFLTAAHPLS* -1.974 0.001933 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23174 LAFLHVGIGCHVTDPCYSDF 20 SLAY-screened peptide P1524 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGCTTTCTTGCACGTTGGCATCGGTTGCCATGTTACGGATCCTTGTTATAGTGATTTCTAA LAFLHVGIGCHVTDPCYSDF* -1.973 0.005717 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23175 RQCNHGNNCGEFLVHCTSFA 20 SLAY-screened peptide P1525 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCAGTGCAATCACGGCAACAATTGCGGTGAGTTCCTTGTGCACTGTACGAGCTTCGCGTAA RQCNHGNNCGEFLVHCTSFA* -1.972 0.000298 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23176 SYIYPH 6 SLAY-screened peptide P1526 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTACATCTATCCCCATTAGTGTTGGCGGCTTCGCTGTATCTACTACGCTCTTAATCATTAA SYIYPH*CWRLRCIYYALNH* -1.971 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23177 YLLPFTVLGPRRRVTPFIRK 20 SLAY-screened peptide P1527 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCTCCTGCCGTTCACGGTGCTTGGGCCTCGCCGGCGTGTTACTCCTTTCATCCGCAAGTAA YLLPFTVLGPRRRVTPFIRK* -1.971 0.002202 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23178 VGTLTRAKAYRVYCCPNRRSN 21 SLAY-screened peptide P1528 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGGCACCCTGACTCGGGCGAAGGCTTATCGGGTCTATTGCTGTCCCAACCGGAGAAGTAAC VGTLTRAKAYRVYCCPNRRSN -1.971 0.000159 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23179 RLCTHPCDRFGDASGRSAVN 20 SLAY-screened peptide P1529 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTTTGCACGCATCCCTGCGACCGTTTTGGCGATGCGTCGGGCCGGTCCGCCGTGAATTAA RLCTHPCDRFGDASGRSAVN* -1.971 0.004502 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23180 STACFDDAGDKFIFRMTLLVN 21 SLAY-screened peptide P1530 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCACTGCTTGTTTTGATGATGCCGGGGATAAATTTATTTTTAGAATGACTCTTTTAGTTAAC STACFDDAGDKFIFRMTLLVN -1.97 0.000395 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23181 HWSPLLLNSFDLDHHYLFYI 20 SLAY-screened peptide P1531 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTGGTCGCCTCTTCTGCTCAATAGTTTCGACCTGGACCATCATTATCTCTTTTATATCTAA HWSPLLLNSFDLDHHYLFYI* -1.97 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23182 SSCAVCAWQYRTCSDCSVTL 20 SLAY-screened peptide P1532 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTCCTGTGCGGTCTGCGCTTGGCAGTACCGTACCTGTTCCGACTGCAGCGTCACCCTCTAA SSCAVCAWQYRTCSDCSVTL* -1.97 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23183 DRPTVFPCYLTQVLTSYVVA 20 SLAY-screened peptide P1533 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCGCCCCACCGTCTTCCCCTGCTACCTTACTCAGGTTCTCACCTCGTATGTCGTTGCGTAA DRPTVFPCYLTQVLTSYVVA* -1.97 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23184 TDHGPNHQPF 10 SLAY-screened peptide P1534 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGACCATGGTCCCAACCATCAGCCCTTTTAGCTTGGCGTTACTGCTAATCCCTATGACTAA TDHGPNHQPF*LGVTANPYD* -1.969 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23185 GARLVLFYPSTTKDQNAPFI 20 SLAY-screened peptide P1535 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGGCTCGCCTCGTGCTTTTTTATCCCAGTACGACCAAGGACCAGAACGCGCCCTTTATTTAA GARLVLFYPSTTKDQNAPFI* -1.968 0.000292 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23186 RPDYALYHKPSDRANWVHYW 20 SLAY-screened peptide P1536 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCTGACTACGCGCTCTATCATAAGCCTTCTGATCGCGCTAATTGGGTCCATTACTGGTAA RPDYALYHKPSDRANWVHYW* -1.968 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23187 HILEFQASHMYVWSNAGSSY 20 SLAY-screened peptide P1537 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATTCTGGAGTTTCAGGCGAGCCACATGTATGTTTGGTCTAACGCCGGTAGTAGTTATTAA HILEFQASHMYVWSNAGSSY* -1.967 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23188 PSTSSGYGFDCRSLFSFNWT 20 SLAY-screened peptide P1538 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTCCACCTCCTCCGGTTATGGGTTTGATTGCAGGAGCCTTTTTAGTTTTAATTGGACGTAA PSTSSGYGFDCRSLFSFNWT* -1.967 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23189 TAVPSPRVTVDKIPTTHVIR 20 SLAY-screened peptide P1539 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGCCGTTCCTTCCCCTAGGGTTACTGTCGACAAGATTCCTACCACCCATGTCATCCGCTAA TAVPSPRVTVDKIPTTHVIR* -1.967 7.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23190 TLVPHYSQDTMRCTLFSHLI 20 SLAY-screened peptide P1540 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTTGGTCCCCCATTATTCTCAGGACACCATGAGGTGCACCCTCTTTAGTCACTTGATTTAA TLVPHYSQDTMRCTLFSHLI* -1.967 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23191 WSYASLTAIPFAVL 14 SLAY-screened peptide P1541 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGAGCTACGCGTCGTTGACCGCCATTCCGTTTGCAGTACTGTGACGACTAGCTAACTGAGTA WSYASLTAIPFAVL*RLAN*V -1.966 0.005069 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23192 WPVHVNSPTCYEVPLNRQLS 20 SLAY-screened peptide P1542 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCCGGTCCACGTCAATAGTCCGACGTGCTACGAGGTCCCCCTTAACAGGCAGCTGAGTTAA WPVHVNSPTCYEVPLNRQLS* -1.966 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23193 LPAGTIAFQYENSSYIVILY 20 SLAY-screened peptide P1543 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCCGCGGGCACCATCGCGTTCCAGTATGAGAACTCCTCCTATATTGTTATTTTGTATTAA LPAGTIAFQYENSSYIVILY* -1.966 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23194 TAPRQNRSVSLR 12 SLAY-screened peptide P1544 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGCCCCCCGGCAGAACAGGAGCGTCTCTCTCCGTTAGGTTAACCTGCCGGCTCCGAACTAA TAPRQNRSVSLR*VNLPAPN* -1.966 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23195 FNSLPYRQLATFCIVVPRSV 20 SLAY-screened peptide P1545 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCAACTCTCTTCCCTACCGTCAGCTGGCGACTTTTTGCATCGTGGTTCCGCGCTCTGTGTAA FNSLPYRQLATFCIVVPRSV* -1.965 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23196 PSFF 4 SLAY-screened peptide P1546 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGCTTTTTTTAGACTTACGGGGCGTGCAATAATGACCATAGCTCCCTTCTGGTCCCCTAA PSFF*TYGACNNDHSSLLVP* -1.965 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23197 SIYHDTPRYGTASYALLGLS 20 SLAY-screened peptide P1547 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCATTTATCACGACACTCCGAGGTATGGGACGGCTTCTTACGCGTTGCTCGGCTTGAGTTAA SIYHDTPRYGTASYALLGLS* -1.965 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23198 FYEGRQSMPSCYYPIVSLLT 20 SLAY-screened peptide P1548 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTACGAGGGTCGTCAGAGCATGCCCAGCTGTTATTACCCTATTGTCAGCCTCCTTACGTAA FYEGRQSMPSCYYPIVSLLT* -1.964 0.002233 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23199 PPTSLPYLCSPLNPVYLQPL 20 SLAY-screened peptide P1549 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTACCTCTCTGCCCTACCTTTGCAGTCCTCTGAATCCGGTCTATCTCCAGCCCCTTTAA PPTSLPYLCSPLNPVYLQPL* -1.964 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23200 SYTFRARTAGRDCSRCGPPL 20 SLAY-screened peptide P1550 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTATACTTTTCGTGCTAGGACTGCTGGTCGGGACTGTTCCCGCTGCGGCCCTCCTCTGTAA SYTFRARTAGRDCSRCGPPL* -1.963 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23201 AASKRKPPHSCGVAGSCACY 20 SLAY-screened peptide P1551 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGCTAGCAAGCGCAAGCCTCCGCATTCTTGTGGTGTTGCTGGCTCTTGCGCTTGTTACTAA AASKRKPPHSCGVAGSCACY* -1.963 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23202 SALLTHARAYANRVAAATEIN 21 SLAY-screened peptide P1552 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGCTCTTCTTACTCATGCTCGTGCGTACGCCAATCGCGTTGCCGCTGCCACTGAGATTAAC SALLTHARAYANRVAAATEIN -1.963 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23203 SYSTMFLSLLFALETANMKC 20 SLAY-screened peptide P1553 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTATAGTACCATGTTCCTGTCGTTGCTTTTTGCGCTGGAGACCGCTAACATGAAGTGTTAA SYSTMFLSLLFALETANMKC* -1.962 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23204 TRHIPSIKQCAVFHSRLPGY 20 SLAY-screened peptide P1554 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCAGGCACATCCCCTCGATCAAGCAGTGTGCGGTTTTTCACAGCAGGCTCCCCGGTTATTAA TRHIPSIKQCAVFHSRLPGY* -1.961 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23205 CSSAGDFFTNPHNINPKSII 20 SLAY-screened peptide P1555 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTCCTCGGCCGGTGATTTTTTCACTAATCCGCATAATATCAACCCCAAGAGCATCATTTAA CSSAGDFFTNPHNINPKSII* -1.961 6.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23206 PRTCS 5 SLAY-screened peptide P1556 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGTACGTGTTCTTAGAATCTGCGGTCTAGCACTAACGCTTTCGCTATTATTCATATTTAA PRTCS*NLRSSTNAFAIIHI* -1.961 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23207 TFLIILLLLSPTSWSLLPPIN 21 SLAY-screened peptide P1557 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTTTCTTATCATACTGCTACTGCTGTCTCCTACATCATGGTCACTTCTACCGCCAATTAAC TFLIILLLLSPTSWSLLPPIN -1.961 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23208 RPSAPIFCLSIHKDVKKQLC 20 SLAY-screened peptide P1558 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCCAGCGCCCCTATCTTTTGTTTGTCCATTCATAAGGATGTCAAGAAGCAGCTTTGCTAA RPSAPIFCLSIHKDVKKQLC* -1.961 0.00011 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23209 SDIHAPKRILQTSNTL 16 SLAY-screened peptide P1559 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGATATTCACGCTCCCAAGAGGATCCTTCAGACGAGTAACACCCTGTAGTACAATTTTTAC SDIHAPKRILQTSNTL*YNFY -1.96 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23210 VKPRPLHDRP 10 SLAY-screened peptide P1560 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTAAACCTCGGCCATTGCATGATCGACCATAACTCGCACGGCCACAACACCAAGTACTAACT VKPRPLHDRP*LARPQHQVLT -1.96 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23211 NGCTSHNYFSRVQDPLQPIR 20 SLAY-screened peptide P1561 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGGCTGCACCTCCCACAACTACTTTTCGCGTGTGCAGGACCCCTTGCAGCCTATCCGCTAA NGCTSHNYFSRVQDPLQPIR* -1.96 0.001464 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23212 HSTRGACVLTYSPYGTHINP 20 SLAY-screened peptide P1562 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTCGACGCGCGGTGCTTGTGTTCTCACTTACAGCCCGTACGGTACCCATATCAATCCGTAA HSTRGACVLTYSPYGTHINP* -1.959 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23213 PGFWSFTPSDYDCHWTHFYL 20 SLAY-screened peptide P1563 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGGTTTTTGGAGCTTCACCCCCTCGGATTATGATTGCCACTGGACGCATTTCTACCTTTAA PGFWSFTPSDYDCHWTHFYL* -1.959 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23214 TVTAPWNPPITNKLLTFPTG 20 SLAY-screened peptide P1564 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTGACCGCCCCCTGGAATCCCCCTATTACGAATAAGCTTCTCACCTTCCCCACGGGCTAA TVTAPWNPPITNKLLTFPTG* -1.958 0.011425 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23215 PLQASHLSRSPFTCLHP 17 SLAY-screened peptide P1565 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTTGCAGGCTAGTCATCTCAGCAGGTCCCCCTTTACTTGCCTCCACCCTTAGTTTCATTAA PLQASHLSRSPFTCLHP*FH* -1.958 0.004853 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23216 HPKYLSRTTHGSISHQYPTA 20 SLAY-screened peptide P1566 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCTAAGTACCTTTCTCGGACTACTCACGGTTCTATCTCCCATCAGTACCCTACTGCCTAA HPKYLSRTTHGSISHQYPTA* -1.958 0.000159 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23217 GLKNAANESRDPMEYTLSCH 20 SLAY-screened peptide P1567 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCTTAAGAATGCTGCGAATGAGAGTAGGGACCCTATGGAGTATACTCTCTCGTGCCATTAA GLKNAANESRDPMEYTLSCH* -1.958 0.002337 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23218 RYLSSFWRSLARFWKRYNNC 20 SLAY-screened peptide P1568 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTACCTTTCCAGTTTTTGGCGCTCTTTGGCCCGGTTTTGGAAGCGTTATAATAACTGCTAA RYLSSFWRSLARFWKRYNNC* -1.957 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23219 RPIRLNPRCYDRPNCSRHWS 20 SLAY-screened peptide P1569 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCCCATCCGGCTTAACCCTAGGTGCTACGATAGGCCTAATTGTAGCCGCCACTGGTCCTAA RPIRLNPRCYDRPNCSRHWS* -1.957 0.0092 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23220 RWTSHAHRRYIPINP 15 SLAY-screened peptide P1570 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTGGACTTCCCATGCTCACCGTCGTTATATTCCCATCAACCCGTAGATTTATGTCTTTTAA RWTSHAHRRYIPINP*IYVF* -1.957 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23221 HVSRYRALL 9 SLAY-screened peptide P1571 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGTCTCCCGGTATCGTGCCCTCTTGTAGCCCGGTAGTTACACGTCCGTGTTTCGGCCCTAA HVSRYRALL*PGSYTSVFRP* -1.957 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23222 STPLALLRTCSTGNHQSFRQ 20 SLAY-screened peptide P1572 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCACTCCTCTCGCTTTGTTGCGTACCTGTAGCACGGGGAATCACCAGTCGTTTCGGCAGTAA STPLALLRTCSTGNHQSFRQ* -1.956 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23223 YVPMPVSLRTRRRGRPI 17 SLAY-screened peptide P1573 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGTTCCTATGCCCGTTTCTCTCCGTACGAGGAGGCGCGGTCGGCCTATCTAGGCTCCTTAA YVPMPVSLRTRRRGRPI*AP* -1.955 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23224 PPGSLDQVNPLSHCMCYIFS 20 SLAY-screened peptide P1574 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTGGCTCTCTCGACCAGGTCAACCCTTTGTCTCATTGTATGTGCTATATTTTTTCCTAA PPGSLDQVNPLSHCMCYIFS* -1.955 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23225 WLLHKSVPLRAVVTVSHNMF 20 SLAY-screened peptide P1575 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCTGCTTCATAAGAGCGTGCCGCTCAGGGCTGTCGTTACTGTGTCGCACAATATGTTCTAA WLLHKSVPLRAVVTVSHNMF* -1.955 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23226 PRICPLTINTHLAF 14 SLAY-screened peptide P1576 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCGCATTTGTCCTCTCACCATCAACACGCATCTTGCTTTTTGAATACTTAGCTTGGGTAAC PRICPLTINTHLAF*ILSLGN -1.955 9.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23227 PAATSGFKPMSHLLPEICTL 20 SLAY-screened peptide P1577 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCTGCGACGAGCGGTTTTAAGCCTATGAGCCATCTCCTCCCCGAGATTTGCACCCTGTAA PAATSGFKPMSHLLPEICTL* -1.954 3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23228 PPDWG 5 SLAY-screened peptide P1578 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTGATTGGGGCTAGTCTGCGATCCTGTTTGGTCTCCATGATACTCATGCTCCCCACTAA PPDWG*SAILFGLHDTHAPH* -1.953 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23229 YPIWYMHPGGPQAYSDIKYM 20 SLAY-screened peptide P1579 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCCCATTTGGTATATGCACCCGGGGGGCCCGCAGGCCTATAGTGACATTAAGTATATGTAA YPIWYMHPGGPQAYSDIKYM* -1.952 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23230 QGACLCPCMTHGYMYDLILL 20 SLAY-screened peptide P1580 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGGCGCGTGCCTTTGCCCGTGCATGACCCACGGCTACATGTATGACTTGATCCTTCTCTAA QGACLCPCMTHGYMYDLILL* -1.952 0.00529 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23231 PFHSYHNTIYAS 12 SLAY-screened peptide P1581 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTCCACAGCTATCACAATACTATTTATGCCTCGTAGACGATGTCCACCACGCGTGATTAA PFHSYHNTIYAS*TMSTTRD* -1.952 0.000913 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23232 NECADVPRRRKPLCCVFDIA 20 SLAY-screened peptide P1582 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGAGTGTGCTGATGTCCCCCGTCGGCGTAAGCCTTTGTGTTGTGTCTTCGATATCGCCTAA NECADVPRRRKPLCCVFDIA* -1.952 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23233 APRNCICYSRMCTSDFFTTI 20 SLAY-screened peptide P1583 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCTCGGAATTGTATCTGCTATAGTCGCATGTGTACCAGTGACTTCTTTACCACCATTTAA APRNCICYSRMCTSDFFTTI* -1.951 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23234 VRVYLCTKANFPMYVSIHPP 20 SLAY-screened peptide P1584 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGAGGGTGTACCTTTGCACGAAGGCCAATTTTCCTATGTATGTCAGCATCCATCCCCCCTAA VRVYLCTKANFPMYVSIHPP* -1.951 0.002189 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23235 LDTAVPYRIGDAVHAT 16 SLAY-screened peptide P1585 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGATACTGCTGTCCCTTACCGTATCGGCGACGCTGTTCACGCCACCTGATTTAAACTAACT LDTAVPYRIGDAVHAT*FKLT -1.951 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23236 APALLHPVQSDRTASHMNAW 20 SLAY-screened peptide P1586 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCCGCCCTTCTGCATCCGGTCCAGAGTGACCGTACTGCTTCGCACATGAATGCTTGGTAA APALLHPVQSDRTASHMNAW* -1.951 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23237 PHPP 4 SLAY-screened peptide P1587 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCATCCCCCCTAGAACGATTGGATTACGCACAATAGCTATATCATTGTGCCTCATGCGTAA PHPP*NDWITHNSYIIVPHA* -1.95 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23238 RLAMEQPLWPCMILMHNDVC 20 SLAY-screened peptide P1588 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCTTGCTATGGAGCAGCCGCTGTGGCCGTGCATGATCCTGATGCATAACGATGTTTGTTAA RLAMEQPLWPCMILMHNDVC* -1.95 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23239 LNTSFYLIGISISIGTSRSY 20 SLAY-screened peptide P1589 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAATACTAGTTTCTATCTTATCGGTATCTCTATCAGTATCGGGACGTCGCGTTCCTATTAA LNTSFYLIGISISIGTSRSY* -1.95 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23240 TWMNSFPLTYS 11 SLAY-screened peptide P1590 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGGATGAACTCTTTTCCTCTCACTTATTCCTAGTGTTCCAATTCCCCTTACAGGGCCTAA TWMNSFPLTYS*CSNSPYRA* -1.949 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23241 QPDAEVPSPMPLCSNWRYLV 20 SLAY-screened peptide P1591 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCCGGATGCGGAGGTTCCTTCCCCCATGCCCCTTTGCTCTAATTGGCGTTACCTCGTCTAA QPDAEVPSPMPLCSNWRYLV* -1.949 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23242 PSVSNWLCRYNTTVADKGL 19 SLAY-screened peptide P1592 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTGTGAGTAACTGGCTTTGCCGGTATAACACCACCGTGGCGGACAAGGGTCTTTAGTAA PSVSNWLCRYNTTVADKGL** -1.949 0.000831 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23243 GTPMSCQIPCCGFGFRFEYL 20 SLAY-screened peptide P1593 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTACGCCGATGAGTTGCCAGATTCCCTGCTGTGGTTTCGGTTTCCGCTTCGAGTATCTGTAA GTPMSCQIPCCGFGFRFEYL* -1.948 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23244 ARFALHTCSHALAFASSS 18 SLAY-screened peptide P1594 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCGGTTCGCCCTTCACACTTGCTCTCATGCGCTGGCTTTCGCGTCTTCGTCTTAGACCTAA ARFALHTCSHALAFASSS*T* -1.948 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23245 VGGDSYHLQRRAHLCEPLQRL 21 SLAY-screened peptide P1595 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGGGGGGGACTCTTATCACCTGCAACGTCGTGCGCATTTATGCGAACCGCTCCAACGATTA VGGDSYHLQRRAHLCEPLQRL -1.948 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23246 PWVYRCVRPLNYPIASSTTCN 21 SLAY-screened peptide P1596 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTGGGTTTATCGTTGTGTTCGTCCTCTCAATTATCCGATTGCTTCAAGTACAACGTGTAAC PWVYRCVRPLNYPIASSTTCN -1.947 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23247 EMIKFHDPFQASWAWTRKDE 20 SLAY-screened peptide P1597 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGATGATTAAGTTCCACGATCCGTTCCAGGCCTCTTGGGCTTGGACCCGCAAGGATGAGTAA EMIKFHDPFQASWAWTRKDE* -1.946 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23248 YYTLGTSITNGVSISTRSNY 20 SLAY-screened peptide P1598 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTACACGTTGGGTACCAGCATTACTAACGGGGTCAGTATTAGTACTCGTAGTAATTACTAA YYTLGTSITNGVSISTRSNY* -1.945 0.000471 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23249 ILLASIITPTTWIIGENSNF 20 SLAY-screened peptide P1599 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTTGCTGGCCAGCATCATCACGCCGACTACTTGGATCATTGGCGAGAATTCTAACTTCTAA ILLASIITPTTWIIGENSNF* -1.945 0.000107 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23250 HSIHIRVVSPWICIHLALSL 20 SLAY-screened peptide P1600 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTCGATCCATATTCGTGTGGTCTCGCCTTGGATTTGCATCCACCTTGCGCTCTCTCTTTAA HSIHIRVVSPWICIHLALSL* -1.944 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23251 IGNY 4 SLAY-screened peptide P1601 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGGTAACTATTAGTTCGTCTTTGCGTATCGGAATCATACTGTTTATTGTCGCAGGCACTAA IGNY*FVFAYRNHTVYCRRH* -1.943 0.000763 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23252 LELTGFEFFSDVTLD 15 SLAY-screened peptide P1602 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGAGCTTACTGGTTTCGAGTTTTTCTCTGACGTTACTCTCGATTAGCCGAATTAGTGCTAA LELTGFEFFSDVTLD*PN*C* -1.942 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23253 HIWLPIPSDDVS 12 SLAY-screened peptide P1603 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACATTTGGCTGCCGATCCCTAGCGATGATGTCAGCTAGAGGTTTCACCCTACTTCTATGTAA HIWLPIPSDDVS*RFHPTSM* -1.94 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23254 GRPVVACRVSIDPTSNRCHT 20 SLAY-screened peptide P1604 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCGCCCTGTGGTTGCGTGCCGTGTGTCTATTGATCCCACGTCGAATCGTTGTCATACCTAA GRPVVACRVSIDPTSNRCHT* -1.94 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23255 KTCSSQSTQVPCTKFKSYGA 20 SLAY-screened peptide P1605 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGACGTGTTCTTCTCAGAGTACGCAGGTCCCGTGCACCAAGTTCAAGTCTTACGGCGCCTAA KTCSSQSTQVPCTKFKSYGA* -1.94 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23256 PLCVTDLHKPYFGTSVNTIL 20 SLAY-screened peptide P1606 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTGTGTGTCACTGATCTCCACAAGCCGTATTTTGGTACCTCTGTTAACACTATTCTTTAA PLCVTDLHKPYFGTSVNTIL* -1.94 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23257 LIHHIYNASTFCIYTFSSID 20 SLAY-screened peptide P1607 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTATTCATCATATCTATAACGCTTCGACGTTTTGTATCTATACGTTTAGTAGCATTGACTAA LIHHIYNASTFCIYTFSSID* -1.939 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23258 LYGHNRRSRDPWKNTKHGTA 20 SLAY-screened peptide P1608 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTATGGCCATAATAGGCGCAGCCGCGATCCGTGGAAGAATACTAAGCATGGTACGGCGTAA LYGHNRRSRDPWKNTKHGTA* -1.938 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23259 AA 2 SLAY-screened peptide P1609 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGCCTAGGGTTGCGATAACCGGGTTTACCCTGACGATACGTTCCCTTTTTAGTCCCCTTAA AA*GCDNRVYPDDTFPF*SP* -1.938 0.002494 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23260 SSWYFIVAHIYLITR 15 SLAY-screened peptide P1610 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAGTTGGTATTTCATTGTCGCTCATATTTACCTCATCACGAGGTAGTTTGTTATCCCCTAA SSWYFIVAHIYLITR*FVIP* -1.937 0.000568 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23261 FSVHSYNNYEPHVSTDSIAT 20 SLAY-screened peptide P1611 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTCCGTGCATTCTTACAATAACTACGAGCCTCACGTCTCTACTGACAGTATTGCTACCTAA FSVHSYNNYEPHVSTDSIAT* -1.937 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23262 PGPNPTIRAPPPDTPFVRFL 20 SLAY-screened peptide P1612 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGGCCCAACCCTACCATTCGCGCGCCTCCTCCGGATACGCCCTTCGTTCGGTTCCTCTAA PGPNPTIRAPPPDTPFVRFL* -1.937 0.000135 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23263 PDYIDVPTGSSSARKHQHML 20 SLAY-screened peptide P1613 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGATTATATTGACGTGCCTACTGGCTCGTCTTCTGCGCGTAAGCACCAGCACATGCTTTAA PDYIDVPTGSSSARKHQHML* -1.936 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23264 PFPLNVPFYSNWNVIAYNPQ 20 SLAY-screened peptide P1614 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTTTCCGTTGAACGTCCCTTTTTATAGCAATTGGAATGTCATTGCCTACAATCCCCAGTAA PFPLNVPFYSNWNVIAYNPQ* -1.936 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23265 PDTLPRVTPPSAHTSESRVS 20 SLAY-screened peptide P1615 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGACACGCTCCCGCGGGTGACGCCTCCTTCCGCCCACACTTCTGAGAGCCGGGTTTCCTAA PDTLPRVTPPSAHTSESRVS* -1.935 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23266 LLRKPMPVPPKKTDPYYKLT 20 SLAY-screened peptide P1616 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGCTGCGCAAGCCTATGCCTGTTCCGCCTAAGAAGACTGATCCTTATTACAAGCTCACCTAA LLRKPMPVPPKKTDPYYKLT* -1.935 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23267 SIGIRARLVTNYRADLLDY 19 SLAY-screened peptide P1617 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCATTGGTATCCGCGCCAGGCTGGTTACTAATTACCGCGCCGACCTTTTGGATTATTAGTAA SIGIRARLVTNYRADLLDY** -1.935 0.00055 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23268 SVVFRYATSTLHPDMLFP 18 SLAY-screened peptide P1618 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGTGGTTTTTCGGTACGCCACGAGTACTCTTCACCCCGACATGCTGTTTCCTTAACTGAGT SVVFRYATSTLHPDMLFP*LS -1.935 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23269 CDDTDLSRFMLFVRHTKKSL 20 SLAY-screened peptide P1619 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGATGATACCGATCTTTCTCGCTTTATGTTGTTCGTTCGCCACACGAAGAAGAGTCTTTAA CDDTDLSRFMLFVRHTKKSL* -1.935 5.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23270 LSVNTPPSELFRVYRMDASM 20 SLAY-screened peptide P1620 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAGCGTCAACACTCCCCCGAGTGAGTTGTTTCGCGTTTATCGCATGGACGCTTCGATGTAA LSVNTPPSELFRVYRMDASM* -1.934 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23271 HTYTHVLTYTNSSSNPKTNA 20 SLAY-screened peptide P1621 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACACCTACACCCATGTCCTTACGTATACGAATTCCTCCTCTAATCCGAAGACCAATGCCTAA HTYTHVLTYTNSSSNPKTNA* -1.934 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23272 CQLTMNVLNLLLRRFVKSAK 20 SLAY-screened peptide P1622 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCAGCTGACGATGAATGTGCTGAACCTCCTGCTCCGGCGGTTTGTCAAGAGCGCCAAGTAA CQLTMNVLNLLLRRFVKSAK* -1.933 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23273 ATIYAHYRS 9 SLAY-screened peptide P1623 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCACCATCTATGCCCATTATAGGTCCTAGTTTCATTGTGGTAACTCCCATGGTTATAATTAA ATIYAHYRS*FHCGNSHGYN* -1.933 0.000446 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23274 PHTNADIVVRTPHSAPELGL 20 SLAY-screened peptide P1624 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCATACTAACGCGGACATTGTTGTGCGCACTCCGCACAGTGCGCCTGAGCTGGGCCTCTAA PHTNADIVVRTPHSAPELGL* -1.933 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23275 GNIKPMRPHVHRVDPCAYYH 20 SLAY-screened peptide P1625 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTAACATCAAGCCCATGAGGCCCCATGTGCATCGCGTCGACCCTTGCGCGTATTATCACTAA GNIKPMRPHVHRVDPCAYYH* -1.932 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23276 AGSSQTFAMKQASCSECH 18 SLAY-screened peptide P1626 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGGTTCTTCCCAGACCTTTGCCATGAAGCAGGCGAGTTGCTCTGAGTGTCATTAGAGCTAA AGSSQTFAMKQASCSECH*S* -1.932 0.004969 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23277 SMGSHDAPNYPRGTAFIILL 20 SLAY-screened peptide P1627 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCATGGGGAGTCACGACGCGCCTAACTATCCCAGGGGGACTGCTTTTATCATCCTCCTGTAA SMGSHDAPNYPRGTAFIILL* -1.932 0.0004 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23278 HLRYLERQHTSILHSSSPSD 20 SLAY-screened peptide P1628 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTTGCGTTACCTTGAGCGGCAGCATACGTCGATTCTCCACAGCTCGAGCCCCTCGGACTAA HLRYLERQHTSILHSSSPSD* -1.931 0.025526 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23279 SAYDDAWIMDNQIDLNRCDI 20 SLAY-screened peptide P1629 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGCCTATGATGATGCGTGGATCATGGACAATCAGATCGATCTTAATCGTTGTGATATCTAA SAYDDAWIMDNQIDLNRCDI* -1.931 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23280 PCLAAVGVRTDVFTNRVCTQ 20 SLAY-screened peptide P1630 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCCTCGCGGCGGTTGGTGTGAGGACTGATGTTTTTACGAACCGCGTTTGCACCCAGTAA PCLAAVGVRTDVFTNRVCTQ* -1.931 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23281 PPLHLYIILLWPVILRMRLN 20 SLAY-screened peptide P1631 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTCTGCACCTTTATATCATTCTGCTATGGCCGGTAATATTGCGGATGCGTCTTAACTGA PPLHLYIILLWPVILRMRLN* -1.93 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23282 CPTPCMRLLCLLVPRQSHLI 20 SLAY-screened peptide P1632 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCCACTCCCTGCATGCGCCTCCTCTGTCTGCTCGTCCCGCGCCAGTCCCATCTCATTTAA CPTPCMRLLCLLVPRQSHLI* -1.93 6.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23283 AQTHETSCQNSIINRF 16 SLAY-screened peptide P1633 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCAGACTCACGAGACCTCCTGCCAGAATTCTATCATTAACCGGTTCTAGCACGGGTAGTAA AQTHETSCQNSIINRF*HG** -1.929 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23284 DTGRPNRHIN 10 SLAY-screened peptide P1634 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACACGGGCCGCCCCAACCGGCATATCAATTAGATCCTCCTCTAGCTTGCCATTTACTAGTAA DTGRPNRHIN*ILL*LAIY** -1.929 5.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23285 TWVMIPTLLAHTPCTGAFNI 20 SLAY-screened peptide P1635 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGGGTCATGATCCCCACGCTTCTTGCTCATACTCCTTGCACTGGTGCGTTTAACATTTAA TWVMIPTLLAHTPCTGAFNI* -1.929 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23286 GSARTAPNNSNNHQSCPVKF 20 SLAY-screened peptide P1636 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCAGCGCTCGTACGGCCCCGAATAACTCTAATAATCATCAGAGTTGTCCCGTTAAGTTCTAA GSARTAPNNSNNHQSCPVKF* -1.929 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23287 LPVAGLP 7 SLAY-screened peptide P1637 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCTGTGGCCGGTCTGCCCTAGCCCATCTACTCCTAGTCTTGTATTCCTTCTTAGTCGTAA LPVAGLP*PIYS*SCIPS*S* -1.928 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23288 SSFYLVTRMSFMSSHLYNKL 20 SLAY-screened peptide P1638 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTCTTTCTATCTCGTTACGCGTATGTCCTTCATGTCCAGCCATCTTTATAATAAGCTTTAA SSFYLVTRMSFMSSHLYNKL* -1.927 6.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23289 VHYCHHRVNYGHRVIR 16 SLAY-screened peptide P1639 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCATTATTGTCACCATCGCGTTAATTACGGCCATCGTGTCATTCGTTAGCCCGTTCCCTAA VHYCHHRVNYGHRVIR*PVP* -1.927 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23290 CHDGEMPPVQ 10 SLAY-screened peptide P1640 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCACGATGGTGAGATGCCGCCCGTTCAGTAGGTTAACGATGGCTGGCGTTAGATTATGTAA CHDGEMPPVQ*VNDGWR*IM* -1.927 0.002701 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23291 HQPSNRPF 8 SLAY-screened peptide P1641 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCAGCCGTCGAACCGTCCTTTTTAGCTTTGTAACCGTTACTAGTCGAACGTCAGCAACTAA HQPSNRPF*LCNRY*SNVSN* -1.927 0.000272 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23292 TVCREPVCYNYIRYHYFHLI 20 SLAY-screened peptide P1642 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTTTGTCGTGAGCCCGTGTGTTATAATTACATCAGGTATCACTACTTTCACCTTATCTAA TVCREPVCYNYIRYHYFHLI* -1.927 3.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23293 GPPAASRPRLRDAPFDF 17 SLAY-screened peptide P1643 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCCCCCTGCCGCTTCTCGTCCCAGGCTTAGGGACGCTCCTTTCGACTTCTAGCGGCTTTAA GPPAASRPRLRDAPFDF*RL* -1.926 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23294 SVPYSPNTSTTACWLLPSIG 20 SLAY-screened peptide P1644 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGTGCCTTATTCCCCGAACACCAGTACCACCGCCTGCTGGTTGCTTCCTTCTATTGGTTAA SVPYSPNTSTTACWLLPSIG* -1.926 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23295 LIVKQSLSNYSTTFSTTGPSN 21 SLAY-screened peptide P1645 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGATTGTCAAGCAGTCGCTCTCTAACTATAGTACTACCTTCAGTACAACAGGTCCATCTAAC LIVKQSLSNYSTTFSTTGPSN -1.926 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23296 WHRYYSWLKRYWTYWKYGHF 20 SLAY-screened peptide P1646 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCACAGGTATTATAGCTGGCTCAAGCGTTATTGGACTTACTGGAAGTATGGTCATTTTTAA WHRYYSWLKRYWTYWKYGHF* -1.926 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23297 RLRSFRRYSCHR 12 SLAY-screened peptide P1647 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCTGAGGTCCTTTCGCCGCTATTCCTGCCACCGGTAGATGGTTCGGCTTGATATGTATTAA RLRSFRRYSCHR*MVRLDMY* -1.925 0.002233 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23298 AEIPCLMRYWHPRSLPVFGA 20 SLAY-screened peptide P1648 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGAGATCCCCTGCCTCATGCGGTACTGGCACCCGCGTTCGTTGCCGGTTTTCGGCGCTTAA AEIPCLMRYWHPRSLPVFGA* -1.925 5.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23299 RLSTAPRWSCLFCVMLNHTY 20 SLAY-screened peptide P1649 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTTAGTACGGCTCCGCGTTGGAGTTGTCTCTTCTGCGTCATGTTGAATCACACCTATTAA RLSTAPRWSCLFCVMLNHTY* -1.925 0.004099 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23300 PLSFTYLLAAPPSLYCGMYY 20 SLAY-screened peptide P1650 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTGTCTTTTACTTATCTCCTCGCTGCTCCTCCGTCTCTTTATTGCGGCATGTATTATTAA PLSFTYLLAAPPSLYCGMYY* -1.925 0.005837 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23301 APRTDHRSHNDLANAKMIAP 20 SLAY-screened peptide P1651 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCTCGCACTGATCACCGGAGTCACAACGACCTCGCGAACGCTAAGATGATCGCCCCCTAA APRTDHRSHNDLANAKMIAP* -1.925 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23302 ICFTMSIRCTPGFLCSTIGP 20 SLAY-screened peptide P1652 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTGCTTTACCATGTCCATTCGTTGCACTCCGGGTTTCCTGTGTTCTACGATTGGCCCTTAA ICFTMSIRCTPGFLCSTIGP* -1.923 0.000106 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23303 LFHSST 6 SLAY-screened peptide P1653 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTTTCATAGCTCCACGTAGGTTCGTAAGTATAGTGCTTTTTGGGCGTGCCTCAGTCACTAA LFHSST*VRKYSAFWACLSH* -1.923 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23304 TGIRLFLRLRAILRRIA 17 SLAY-screened peptide P1654 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACAGGAATTCGATTGTTTTTACGATTACGAGCAATATTACGCAGAATTGCATGAGTCATTAAC TGIRLFLRLRAILRRIA*VIN -1.922 0.015523 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23305 RRTPNGS 7 SLAY-screened peptide P1655 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCGTACGCCGAACGGCTCCTAGGTCCGGGACCTTACGAGCAGCACCCAGCCGGCTGTGTAA RRTPNGS*VRDLTSSTQPAV* -1.922 0.000166 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23306 PLWPPSIPDIFHSITG 16 SLAY-screened peptide P1656 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCTGGCCTCCGTCCATTCCTGATATTTTTCACAGTATCACGGGCTAGCCGACGTGTTAA PLWPPSIPDIFHSITG*PTC* -1.921 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23307 SIGSVDFIAAMPHATPHWSG 20 SLAY-screened peptide P1657 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTATTGGTTCGGTGGATTTCATTGCGGCGATGCCTCATGCTACCCCCCATTGGTCCGGCTAA SIGSVDFIAAMPHATPHWSG* -1.921 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23308 INRYIIFAICYPRRKWARAD 20 SLAY-screened peptide P1658 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAACCGGTACATCATCTTCGCTATTTGTTATCCGCGCCGCAAGTGGGCTAGGGCTGATTAA INRYIIFAICYPRRKWARAD* -1.921 5.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23309 YSFAGHNPEYLALG 14 SLAY-screened peptide P1659 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTCGTTTGCTGGGCACAATCCCGAGTACCTCGCGCTTGGCTAGCTGTCCATTAAGGTCTAA YSFAGHNPEYLALG*LSIKV* -1.921 9.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23310 CNYVLDLVHAHMPNPARIRT 20 SLAY-screened peptide P1660 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTAACTATGTTCTTGATCTTGTTCATGCCCATATGCCCAACCCCGCCCGTATCCGCACTTAA CNYVLDLVHAHMPNPARIRT* -1.92 0.006176 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23311 FYLFGFFLVLDLRNKILFSIN 21 SLAY-screened peptide P1661 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTACCTGTTCGGGTTTTTCCTTGTTCTCGACCTCCGTAACAAGATACTATTCTCCATTAAC FYLFGFFLVLDLRNKILFSIN -1.92 0.00118 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23312 PQLTRPEPHISAKGTAAAAP 20 SLAY-screened peptide P1662 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCAGCTCACGCGTCCGGAGCCCCACATTTCGGCTAAGGGCACTGCTGCTGCTGCGCCTTAA PQLTRPEPHISAKGTAAAAP* -1.918 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23313 IAIPSLTFDRIVTGGMAHNQ 20 SLAY-screened peptide P1663 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGCTATTCCGTCTCTCACTTTTGACCGTATTGTTACCGGTGGCATGGCGCACAATCAGTAA IAIPSLTFDRIVTGGMAHNQ* -1.918 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23314 RSAYIPITASYFRIAHMP 18 SLAY-screened peptide P1664 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTCCGCCTACATCCCCATTACGGCTTCGTACTTTCGTATCGCCCATATGCCGTAGGGCTAA RSAYIPITASYFRIAHMP*G* -1.918 0.000227 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23315 SPYFHLYTPYLNRLLTRTPV 20 SLAY-screened peptide P1665 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCCCTACTTTCATCTGTATACTCCGTATCTCAACCGCCTGCTCACTAGGACCCCGGTCTAA SPYFHLYTPYLNRLLTRTPV* -1.916 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23316 YNLFHPNLQTIIMSIN 16 SLAY-screened peptide P1666 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACAACTTGTTTCACCCGAATCTCCAGACTATTATCATGTCTATTAATTAGTATTCTAACTAA YNLFHPNLQTIIMSIN*YSN* -1.915 0.003473 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23317 PPGIGDHTVCSLH 13 SLAY-screened peptide P1667 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTGGTATCGGTGACCATACCGTGTGTTCCTTGCACTAGGCTCAGAACTTTGCCGTTTAA PPGIGDHTVCSLH*AQNFAV* -1.915 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23318 HSDVYCFTSSNHIILGVNYN 20 SLAY-screened peptide P1668 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAGCGACGTCTACTGCTTTACGAGTTCGAACCATATTATTCTGGGCGTTAACTATAATTAA HSDVYCFTSSNHIILGVNYN* -1.915 0.000717 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23319 ARLIYSPLSCQPPVLWFATN 20 SLAY-screened peptide P1669 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAGGCTCATCTATTCCCCCCTCTCTTGTCAGCCCCCCGTGCTTTGGTTTGCGACTAATTAA ARLIYSPLSCQPPVLWFATN* -1.915 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23320 RRSTVKPHHSQVHNPTYANA 20 SLAY-screened peptide P1670 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCGCTCCACTGTCAAGCCCCACCATTCGCAGGTCCATAACCCCACTTATGCGAATGCGTAA RRSTVKPHHSQVHNPTYANA* -1.914 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23321 PLYD 4 SLAY-screened peptide P1671 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTGTATGACTAGCGTGGTCACCATCCTTTTGGCCTTCTCAACCCGAATTCCCCCAGGTAA PLYD*RGHHPFGLLNPNSPR* -1.914 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23322 TVESTMDHRSAASTTNSRNT 20 SLAY-screened peptide P1672 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTCGAGAGCACCATGGACCACCGGTCCGCGGCTAGTACTACTAATTCTCGGAACACCTAA TVESTMDHRSAASTTNSRNT* -1.913 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23323 PTL 3 SLAY-screened peptide P1673 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGACTCTCTAGCTCGTCTTCCCTACTAGTCTTAATTGCTAGCAGCTCAATATTATGATGTAA PTL*LVFPTSLNC*QLNIMM* -1.913 0.006062 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23324 DDHRYLSNPFIMCWTDCSIT 20 SLAY-screened peptide P1674 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGACCATCGCTACCTTTCTAATCCTTTTATTATGTGTTGGACTGACTGTTCTATTACGTAA DDHRYLSNPFIMCWTDCSIT* -1.913 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23325 CHYLMDNPYFIYV 13 SLAY-screened peptide P1675 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCACTATCTTATGGACAACCCCTATTTCATTTATGTCTAGCTCCTCAAGCTTGCGGGTAAC CHYLMDNPYFIYV*LLKLAGN -1.913 0.000159 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23326 SALIHYYWGGGYFLSGPLVP 20 SLAY-screened peptide P1676 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGCGCTCATCCACTACTATTGGGGCGGTGGGTATTTTTTGTCCGGGCCTCTGGTCCCTTAA SALIHYYWGGGYFLSGPLVP* -1.913 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23327 GTFYGSSLARQSSCH 15 SLAY-screened peptide P1677 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTACTTTCTATGGGTCGTCGCTCGCCCGTCAGTCCTCGTGCCATTAGTACTCTCGTAAGTAA GTFYGSSLARQSSCH*YSRK* -1.912 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23328 AAADRINTDTSVHSSDMTNT 20 SLAY-screened peptide P1678 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGCTGCGGATCGTATTAACACTGATACTTCCGTCCATAGTTCTGATATGACCAATACCTAA AAADRINTDTSVHSSDMTNT* -1.912 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23329 LN 2 SLAY-screened peptide P1679 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAACTAGCTTCCGTATGCTAGCGCCCTGCAGAATGGTTTTCGGGATGTCGTGCACTGTTAA LN*LPYASALQNGFRDVVHC* -1.912 7.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23330 FDLSIHIVIIINNWTLNFHF 20 SLAY-screened peptide P1680 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTGACCTTTCGATCCACATTGTGATTATCATCAATAATTGGACCCTCAATTTCCACTTCTAA FDLSIHIVIIINNWTLNFHF* -1.912 0.000779 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23331 CTKQYFSLDTSFWNWPTYLL 20 SLAY-screened peptide P1681 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCACGAAGCAGTACTTTAGTCTCGATACTTCCTTTTGGAACTGGCCCACCTACCTCCTTTAA CTKQYFSLDTSFWNWPTYLL* -1.91 8.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23332 YHTDPSGMPPDSVFLIKHYC 20 SLAY-screened peptide P1682 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCATACTGATCCTAGTGGGATGCCCCCCGATTCGGTTTTTCTGATTAAGCATTATTGCTAA YHTDPSGMPPDSVFLIKHYC* -1.91 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23333 RYLAHPHSPVVFPPRRNSRT 20 SLAY-screened peptide P1683 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTATCTTGCGCACCCTCACTCTCCTGTTGTTTTCCCGCCTCGTAGGAATAGCCGTACCTAA RYLAHPHSPVVFPPRRNSRT* -1.91 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23334 PRWLSCKNNTNYYMTKNVAS 20 SLAY-screened peptide P1684 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGGTGGCTGTCGTGTAAGAACAATACCAATTATTACATGACCAAGAATGTTGCTAGTTAA PRWLSCKNNTNYYMTKNVAS* -1.91 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23335 DLVFTLVTVGGLTITILPVGN 21 SLAY-screened peptide P1685 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCTTGTGTTTACTCTCGTTACAGTTGGTGGCCTCACGATTACAATACTGCCTGTGGGTAAC DLVFTLVTVGGLTITILPVGN -1.91 0.00018 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23336 RVAPFTSDGDGFSNLSFCTRN 21 SLAY-screened peptide P1686 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGTGGCGCCCTTCACCAGCGATGGTGATGGTTTTAGTAATCTGAGTTTCTGTACACGTAAC RVAPFTSDGDGFSNLSFCTRN -1.909 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23337 PPSTNSAGSFWNGCFIAIGA 20 SLAY-screened peptide P1687 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCGTCTACCAATAGTGCGGGGTCGTTTTGGAATGGTTGTTTCATTGCGATCGGCGCTTAA PPSTNSAGSFWNGCFIAIGA* -1.909 0.000441 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23338 CLERIFVYLPPSTTV 15 SLAY-screened peptide P1688 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCTCGAGCGTATTTTCGTTTATCTGCCTCCGTCCACTACCGTGTAGATTCGTTCTCCGTAA CLERIFVYLPPSTTV*IRSP* -1.908 2.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23339 ALTTTLSRPHIYRSAAWSDN 20 SLAY-screened peptide P1689 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCTGACCACCACTCTTTCCCGTCCGCATATCTACCGCTCGGCTGCCTGGTCTGACAACTAA ALTTTLSRPHIYRSAAWSDN* -1.907 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23340 AGPVAPNPQPSWSPLNAILI 20 SLAY-screened peptide P1690 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGGCCCCGTTGCCCCTAATCCCCAGCCCTCCTGGTCGCCCCTGAATGCTATCCTGATTTAA AGPVAPNPQPSWSPLNAILI* -1.906 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23341 CSIYNRTVCFNLFLPITNPV 20 SLAY-screened peptide P1691 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAGCATTTACAATCGTACGGTTTGTTTTAACCTTTTTCTGCCTATTACCAACCCTGTGTAA CSIYNRTVCFNLFLPITNPV* -1.906 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23342 AAAVAAT 7 SLAY-screened peptide P1692 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGCGGCCGTTGCCGCCACTTAGAACACCATTTTTCATCTGCATCGCTGCAACGCCAATTAA AAAVAAT*NTIFHLHRCNAN* -1.906 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23343 IQNVRPTSMPERRPSLDKDN 20 SLAY-screened peptide P1693 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCAGAACGTCCGCCCTACGTCCATGCCTGAGCGTAGGCCCTCGCTGGATAAGGATAATTAA IQNVRPTSMPERRPSLDKDN* -1.905 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23344 PLQDSDTPYSAVCSRLTAPR 20 SLAY-screened peptide P1694 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCCAGGATAGCGACACCCCTTATTCCGCCGTGTGTAGCCGCCTTACCGCGCCTCGTTAA PLQDSDTPYSAVCSRLTAPR* -1.905 0.006535 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23345 LRADATSDSRSQRPSRRTDF 20 SLAY-screened peptide P1695 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCGGGCCGATGCCACGTCTGACTCGCGTAGTCAGCGTCCTTCGCGTCGGACTGACTTCTAA LRADATSDSRSQRPSRRTDF* -1.904 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23346 HPFPGDWCPDSCNNQVIYSR 20 SLAY-screened peptide P1696 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCTTTCCCCGGTGATTGGTGCCCGGACTCCTGCAACAACCAGGTCATCTATTCTCGCTAA HPFPGDWCPDSCNNQVIYSR* -1.904 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23347 PTCFLAATFHFWMNLRPGGG 20 SLAY-screened peptide P1697 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACGTGTTTTTTGGCCGCCACCTTTCACTTCTGGATGAACCTCCGCCCGGGTGGCGGGTAA PTCFLAATFHFWMNLRPGGG* -1.904 0.001296 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23348 LLFYISPLYCEICDKDLEWS 20 SLAY-screened peptide P1698 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCTTTTTTACATTTCCCCCCTTTACTGTGAGATTTGCGATAAGGATCTCGAGTGGTCTTAA LLFYISPLYCEICDKDLEWS* -1.904 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23349 LRVYVPLRTDFVNCAHFSMY 20 SLAY-screened peptide P1699 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCGCGTGTACGTGCCGCTTAGGACCGACTTTGTCAATTGTGCTCATTTCTCGATGTACTAA LRVYVPLRTDFVNCAHFSMY* -1.903 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23350 VYFRPMRGCPPEGAGQNSFD 20 SLAY-screened peptide P1700 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTATTTCCGGCCCATGCGCGGGTGCCCCCCTGAGGGTGCCGGCCAGAACTCCTTCGACTAA VYFRPMRGCPPEGAGQNSFD* -1.903 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23351 PSRSFRSCPEAYYYWAQLYF 20 SLAY-screened peptide P1701 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTCTCGTTCTTTCCGTTCCTGCCCTGAGGCTTACTATTACTGGGCTCAGCTGTATTTCTAA PSRSFRSCPEAYYYWAQLYF* -1.903 0.00029 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23352 IH 2 SLAY-screened peptide P1702 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCACTAGCTGGCGAAGTAGACGACCCGCCTTATCCGTTTGGCGCCTCATTCGAAGTACTAA IH*LAK*TTRLIRLAPHSKY* -1.903 0.012041 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23353 HDAVWQPPKPALTSNTIVRD 20 SLAY-screened peptide P1703 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGATGCGGTTTGGCAGCCGCCGAAGCCTGCCCTCACCAGTAATACGATTGTTCGTGATTAA HDAVWQPPKPALTSNTIVRD* -1.903 0.004043 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23354 YQRERIPLSCFTAVCKLTIM 20 SLAY-screened peptide P1704 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCAGCGCGAGCGCATCCCTCTTTCGTGTTTCACGGCTGTTTGCAAGCTTACGATCATGTAA YQRERIPLSCFTAVCKLTIM* -1.902 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23355 GSHKLLTTNKVLLSLAICPM 20 SLAY-screened peptide P1705 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCAGCCATAAGCTTCTGACTACTAACAAGGTCTTGCTGTCCCTCGCCATCTGTCCTATGTAA GSHKLLTTNKVLLSLAICPM* -1.902 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23356 DVATTRNNLSIFRYKCQSFP 20 SLAY-screened peptide P1706 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGTTGCCACTACCAGGAACAATCTTTCCATTTTCCGCTATAAGTGCCAGTCGTTTCCTTAA DVATTRNNLSIFRYKCQSFP* -1.901 0.000321 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23357 PLFTLGKRIINHLLRAYKYD 20 SLAY-screened peptide P1707 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTTTTACTCTGGGTAAGAGGATCATCAACCACTTGCTCAGGGCTTACAAGTATGACTAA PLFTLGKRIINHLLRAYKYD* -1.901 0.000232 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23358 SLWLRLSSSISLIVTALTLTN 21 SLAY-screened peptide P1708 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTTGTGGCTGCGACTCTCCTCGTCGATCTCCCTGATAGTGACTGCGCTTACTTTAACTAAC SLWLRLSSSISLIVTALTLTN -1.9 6.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23359 GYLSGAPCYYQL 12 SLAY-screened peptide P1709 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTACTTGTCGGGAGCCCCATGTTACTACCAACTCTGATAACTTTCTGACGTGTTAACTGAG GYLSGAPCYYQL**LSDVLTE -1.9 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23360 QFRVHQVYLRYFAPLYHSIS 20 SLAY-screened peptide P1710 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTTCCGTGTTCACCAGGTGTATCTGCGGTATTTCGCTCCCCTGTACCATTCTATTAGTTAA QFRVHQVYLRYFAPLYHSIS* -1.899 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23361 GWISNLPAVMMNPHNLPAWY 20 SLAY-screened peptide P1711 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTGGATTTCCAATCTTCCCGCTGTTATGATGAATCCTCATAATCTGCCCGCGTGGTACTAA GWISNLPAVMMNPHNLPAWY* -1.898 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23362 GQIKPIIMGCQWSGNHFLTS 20 SLAY-screened peptide P1712 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCAGATTAAGCCCATTATTATGGGTTGTCAGTGGAGTGGTAATCATTTCCTTACCTCTTAA GQIKPIIMGCQWSGNHFLTS* -1.898 0.003662 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23363 LATLSAACTSAP 12 SLAY-screened peptide P1713 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGGCCACCTTGAGTGCTGCCTGCACGTCCGCCCCTTAGCTGATTAACACCACTCACTTTTAA LATLSAACTSAP*LINTTHF* -1.898 0.006087 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23364 ACGQ 4 SLAY-screened peptide P1714 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGTGCGGGCAGTAGAATTCTGCCACTTTTGGGCCCTCGCTTGACAAGCAGTTTTATACCTAA ACGQ*NSATFGPSLDKQFYT* -1.898 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23365 SLLNAP 6 SLAY-screened peptide P1715 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCTGCTTAACGCTCCGTAGTTGTTGCGCGACACTATTCACTACGACGCCGCGTCCGTTTAA SLLNAP*LLRDTIHYDAASV* -1.898 0.000206 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23366 IADFVPSLRVHFTPFWQTNA 20 SLAY-screened peptide P1716 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGCTGATTTCGTGCCCAGTCTGCGTGTGCACTTTACTCCCTTTTGGCAGACTAACGCCTAA IADFVPSLRVHFTPFWQTNA* -1.897 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23367 PGPLHATFRCPTIIIPTTTSN 21 SLAY-screened peptide P1717 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGTCCGCTCCATGCTACGTTCCGATGTCCAACCATTATTATCCCCACGACTACTTCTAAC PGPLHATFRCPTIIIPTTTSN -1.896 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23368 RNAS 4 SLAY-screened peptide P1718 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGAATGCGAGTTAGTCCCTCTATATTAATATTAGTCTCGCGCATCTCACTTACAACATTTAA RNAS*SLYINISLAHLTYNI* -1.896 0.000132 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23369 HLTEHGWTWRCVDIINKYNNY 21 SLAY-screened peptide P1719 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCTCACTGAGCACGGCTGGACCTGGCGCTGCGTTGACATTATTAATAAGTACAATAATTAC HLTEHGWTWRCVDIINKYNNY -1.896 0.00039 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23370 PPPVARHPCFKHVSVDSYRT 20 SLAY-screened peptide P1720 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTCCGGTTGCGCGCCATCCGTGTTTTAAGCACGTGTCTGTTGACTCTTATCGCACGTAA PPPVARHPCFKHVSVDSYRT* -1.896 0.029669 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23371 TNPNYRHNRSALEFTNIWFH 20 SLAY-screened peptide P1721 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAACCCCAATTATCGTCACAATAGGTCCGCGCTCGAGTTCACCAACATCTGGTTTCACTAA TNPNYRHNRSALEFTNIWFH* -1.895 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23372 HKKAGYSSGEWPRAFVFAPA 20 SLAY-screened peptide P1722 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAAGAAGGCGGGCTACTCTTCGGGTGAGTGGCCCAGGGCGTTCGTCTTCGCGCCTGCGTAA HKKAGYSSGEWPRAFVFAPA* -1.895 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23373 TTAFICILRRYRRDAYQIYLL 21 SLAY-screened peptide P1723 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGACCGCCTTTATTTGCATTCTTAGGCGCTATCGTCGCGATGCCTATCAGATCTACCTCCTA TTAFICILRRYRRDAYQIYLL -1.894 2.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23374 HYPPFGIFDMINCINSEITN 20 SLAY-screened peptide P1724 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTACCCCCCCTTCGGGATCTTCGACATGATCAATTGCATCAACTCCGAGATTACTAATTAA HYPPFGIFDMINCINSEITN* -1.894 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23375 NPGSIPAVYQSRHLTSPKCP 20 SLAY-screened peptide P1725 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCCCGGGTCTATCCCGGCTGTCTACCAGTCCAGGCACCTCACTTCCCCTAAGTGCCCGTAA NPGSIPAVYQSRHLTSPKCP* -1.893 0.000848 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23376 TTVTYLLSPFYYAGSIAGGF 20 SLAY-screened peptide P1726 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCACCGTCACTTATCTCCTCTCGCCTTTCTACTATGCGGGTTCGATCGCCGGCGGGTTTTAA TTVTYLLSPFYYAGSIAGGF* -1.893 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23377 VLPGGPPWLHASTRYASEVS 20 SLAY-screened peptide P1727 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCTCCCTGGGGGCCCGCCTTGGCTGCATGCGTCTACTCGCTACGCTAGTGAGGTGTCCTAA VLPGGPPWLHASTRYASEVS* -1.893 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23378 TGTPAACYTSHYYRPAYNIS 20 SLAY-screened peptide P1728 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGGGACTCCTGCGGCCTGTTACACTAGTCATTATTACCGCCCCGCGTATAACATCAGTTAA TGTPAACYTSHYYRPAYNIS* -1.892 0.006083 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23379 HTSSRDCNIPVHLPYPHISL 20 SLAY-screened peptide P1729 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACGTCTAGCCGTGATTGTAACATTCCGGTCCATCTTCCCTACCCGCACATCTCTCTTTAA HTSSRDCNIPVHLPYPHISL* -1.892 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23380 DHGTKTGTYFPNCMNLTYML 20 SLAY-screened peptide P1730 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCACGGCACGAAGACTGGCACCTATTTTCCCAATTGTATGAATCTTACTTACATGCTTTAA DHGTKTGTYFPNCMNLTYML* -1.892 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23381 SWQTIQIFSL 10 SLAY-screened peptide P1731 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTGGCAGACCATCCAGATCTTTTCGTTGTAGCCTATCCTCCCCCCTCTCTGCCGGTGCTAA SWQTIQIFSL*PILPPLCRC* -1.891 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23382 RPAITCFHNRYLPSFTMEPL 20 SLAY-screened peptide P1732 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCGGCTATTACGTGTTTTCATAACAGGTACCTTCCTAGTTTTACGATGGAGCCTTTGTAA RPAITCFHNRYLPSFTMEPL* -1.891 0.000872 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23383 LSDVPYHVSAPKDPQCQNGG 20 SLAY-screened peptide P1733 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTCGGACGTGCCCTACCATGTCTCGGCGCCCAAGGACCCCCAGTGTCAGAACGGTGGGTAA LSDVPYHVSAPKDPQCQNGG* -1.891 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23384 PPSHRHDSNFYIALCISIICN 21 SLAY-screened peptide P1734 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCCTCGCACCGGCACGACTCTAACTTTTATATTGCCCTGTGCATCTCGATTATCTGTAAC PPSHRHDSNFYIALCISIICN -1.891 0.003733 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23385 CSTENKNYNNLRDIIPSQLI 20 SLAY-screened peptide P1735 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTCCACTGAGAACAAGAATTATAATAATCTGCGCGACATCATCCCTTCTCAGCTCATCTAA CSTENKNYNNLRDIIPSQLI* -1.891 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23386 RNKGHQTRYTANGGQYREHP 20 SLAY-screened peptide P1736 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGAACAAGGGCCATCAGACCCGGTACACCGCCAACGGTGGCCAGTATCGGGAGCATCCGTAA RNKGHQTRYTANGGQYREHP* -1.89 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23387 HLSNCSPDQIRP 12 SLAY-screened peptide P1737 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCTCAGCAATTGTAGTCCGGACCAGATTAGGCCTTAGATTCACGCGGCTGATAACTATTAA HLSNCSPDQIRP*IHAADNY* -1.89 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23388 PAHLNPFVTPYLIEASEPGS 20 SLAY-screened peptide P1738 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCGCATTTGAATCCTTTCGTTACCCCCTATCTTATCGAGGCTAGTGAGCCTGGGAGCTAA PAHLNPFVTPYLIEASEPGS* -1.889 0.000755 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23389 TNRSAYPGTHYERSYLYYVI 20 SLAY-screened peptide P1739 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAATCGCAGCGCTTACCCGGGTACTCACTACGAGAGGTCCTACCTGTATTACGTGATCTAA TNRSAYPGTHYERSYLYYVI* -1.889 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23390 PLRGLAGTPLIHLYTIHSSY 20 SLAY-screened peptide P1740 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTGCGTGGCCTGGCTGGCACCCCGCTTATCCATCTTTATACCATTCACAGTTCTTATTAA PLRGLAGTPLIHLYTIHSSY* -1.889 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23391 PTYALICYSYRQATSPTATK 20 SLAY-screened peptide P1741 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACCTACGCGCTGATCTGTTACTCGTATCGCCAGGCCACCAGTCCGACTGCGACGAAGTAA PTYALICYSYRQATSPTATK* -1.888 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23392 KHDSYRPRGFLNTTFEIDQS 20 SLAY-screened peptide P1742 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCACGATTCCTACCGCCCGCGCGGCTTCCTCAATACTACGTTCGAGATTGATCAGAGCTAA KHDSYRPRGFLNTTFEIDQS* -1.888 0.012824 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23393 PSYCVPLLDRL 11 SLAY-screened peptide P1743 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGCTACTGTGTCCCGCTGCTCGACCGGTTGTAGCATAGTTCTTACCTGGACGTCCATTAA PSYCVPLLDRL*HSSYLDVH* -1.888 0.001088 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23394 IAVTFPLVTSTPHGHSRLSD 20 SLAY-screened peptide P1744 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGCGGTTACGTTTCCTCTTGTGACGAGTACCCCTCACGGTCATTCTAGGCTGAGTGATTAA IAVTFPLVTSTPHGHSRLSD* -1.888 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23395 CGTSVRPIIPHKHCYDVTTP 20 SLAY-screened peptide P1745 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGGCACTTCGGTCCGTCCTATTATTCCTCATAAGCATTGCTACGATGTGACCACTCCTTAA CGTSVRPIIPHKHCYDVTTP* -1.887 5.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23396 IGSGNADTYLHGSLNQHLHC 20 SLAY-screened peptide P1746 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGGGTCGGGTAACGCTGATACGTATCTTCACGGTTCTCTTAATCAGCACCTTCATTGCTAA IGSGNADTYLHGSLNQHLHC* -1.887 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23397 SHTPPLDNSISFDSDTTYYF 20 SLAY-screened peptide P1747 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCACACCCCGCCGCTGGACAACAGCATCTCGTTTGATAGCGATACGACGTACTACTTCTAA SHTPPLDNSISFDSDTTYYF* -1.887 2.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23398 PSILSYGSYCHPTWHDFGSP 20 SLAY-screened peptide P1748 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGCATCCTGTCCTATGGTAGTTATTGTCACCCTACTTGGCATGATTTCGGTTCTCCCTAA PSILSYGSYCHPTWHDFGSP* -1.886 0.021084 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23399 PTFESNLKETLSNTATRSSV 20 SLAY-screened peptide P1749 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGACTTTTGAGAGCAACCTTAAGGAGACTTTGTCTAACACTGCTACGCGTAGTTCGGTCTAA PTFESNLKETLSNTATRSSV* -1.885 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23400 SGFPYISPPYISNCNFNHDT 20 SLAY-screened peptide P1750 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGGCTTCCCGTATATTTCTCCGCCCTACATCTCGAACTGCAACTTCAACCACGACACGTAA SGFPYISPPYISNCNFNHDT* -1.885 0.005445 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23401 NRKPRRFTCYLNAVLHTSPP 20 SLAY-screened peptide P1751 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCGTAAGCCTCGCCGTTTCACCTGTTACCTGAACGCTGTTCTTCATACCTCTCCGCCGTAA NRKPRRFTCYLNAVLHTSPP* -1.885 0.000213 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23402 HGPGARSFRYHDHNRRCDGR 20 SLAY-screened peptide P1752 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGGCCCTGGTGCCCGTAGTTTTCGTTATCACGATCACAACCGTCGTTGTGACGGTAGGTAA HGPGARSFRYHDHNRRCDGR* -1.885 0.000132 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23403 HRCYHSSPSFYDFFIWRFPW 20 SLAY-screened peptide P1753 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGCTGTTATCACTCTAGCCCGAGTTTTTACGATTTTTTTATTTGGCGTTTCCCTTGGTAA HRCYHSSPSFYDFFIWRFPW* -1.884 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23404 FHRPQDQSHSYTPTVATCTQ 20 SLAY-screened peptide P1754 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCACAGGCCGCAGGATCAGTCTCATTCGTATACTCCCACCGTTGCGACGTGCACGCAGTAA FHRPQDQSHSYTPTVATCTQ* -1.884 0.000278 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23405 PHSNSQPDSRHYPHCWYALL 20 SLAY-screened peptide P1755 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCACAGTAATTCGCAGCCCGATTCCCGGCACTACCCCCACTGTTGGTATGCGCTTCTTTAA PHSNSQPDSRHYPHCWYALL* -1.883 0.006844 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23406 FTYQATHPAPELREASTIGS 20 SLAY-screened peptide P1756 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCACGTACCAGGCCACTCATCCCGCGCCGGAGCTTCGGGAGGCTAGTACGATTGGTAGTTAA FTYQATHPAPELREASTIGS* -1.883 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23407 SNYVRYK 7 SLAY-screened peptide P1757 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCAACTATGTCAGGTATAAGTAGCCCTTGCGTGTTAATCATGAGAGCCGGACCATCTTCTAA SNYVRYK*PLRVNHESRTIF* -1.883 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23408 SQRLAHYKHDNFNVYKHHYS 20 SLAY-screened peptide P1758 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCAGCGCCTTGCGCATTACAAGCATGACAATTTCAATGTGTATAAGCACCATTATTCTTAA SQRLAHYKHDNFNVYKHHYS* -1.883 0.002852 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23409 ARSETCFRPRPGSSSRLGYV 20 SLAY-screened peptide P1759 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCGTTCCGAGACCTGCTTTCGGCCTCGTCCCGGTAGCTCTTCTCGTCTCGGTTATGTCTAA ARSETCFRPRPGSSSRLGYV* -1.881 8.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23410 LPGCYTHCHDHHQPLRRPALN 21 SLAY-screened peptide P1760 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCGGGCTGCTATACGCACTGTCATGATCACCATCAGCCGTTACGAAGACCAGCCCTTAAC LPGCYTHCHDHHQPLRRPALN -1.881 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23411 HCMLLLA 7 SLAY-screened peptide P1761 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGTATGTTGCTTCTTGCGTAGGTGACGACCGCTATGTGGCGTGTTCACTGCCTGATTTAA HCMLLLA*VTTAMWRVHCLI* -1.881 0.002946 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23412 TRTRTLIILLRIFSRLIRSSN 21 SLAY-screened peptide P1762 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGAGAACCAGAACCCTTATTATATTATTGAGAATTTTTTCGAGACTAATACGCAGCAGTAAC TRTRTLIILLRIFSRLIRSSN -1.881 8.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23413 WILS 4 SLAY-screened peptide P1763 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGATTCTCTCTTAGTACTTCTCCAACCCCACTGACAGGTTTCCGCTGTAGGACCATCCTTAA WILS*YFSNPTDRFPL*DHP* -1.881 0.01302 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23414 HTILSSIAADDMVIRANTPVN 21 SLAY-screened peptide P1764 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACACTATCCTCTCTAGTATCGCCGCGGATGATATGGTTATTAGGGCTAATACGCCGGTTAAC HTILSSIAADDMVIRANTPVN -1.88 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23415 HVRPLTQNSIDNNLSLSRAH 20 SLAY-screened peptide P1765 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGTGCGTCCTCTTACCCAGAATTCGATTGACAATAACCTTTCTCTCTCCCGCGCGCATTAA HVRPLTQNSIDNNLSLSRAH* -1.88 0.000249 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23416 IN 2 SLAY-screened peptide P1766 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAACTAGCTTTCTACTAATACTTAGGGCCCTTTTGATAACGTTGTGCTTCCCACTCCCTAA IN*LSTNT*GPFDNVVLPTP* -1.88 0.000183 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23417 LMISNRHG 8 SLAY-screened peptide P1767 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCATGATCTCGAATCGGCATGGTTAGTACAATTCCCGCAACGATCTCCTCGCCTGCAGCTAA LMISNRHG*YNSRNDLLACS* -1.879 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23418 MTCTG 5 SLAY-screened peptide P1768 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGACTTGCACCGGCTAGCCGGGTCTCTCCCCCCCTCCGCTCGGTTCTATTTTTGACCCGTAA MTCTG*PGLSPPPLGSIFDP* -1.878 5.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23419 DLYAS 5 SLAY-screened peptide P1769 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCTTTATGCCAGCTAGTTTCATAGCACTGACCCCCCTTACATTGATGTGTTGGTTATTTAA DLYAS*FHSTDPPYIDVLVI* -1.878 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23420 SVRHQHLLLCVRGTCLLKFK 20 SLAY-screened peptide P1770 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGTCCGGCATCAGCATCTGCTTTTGTGTGTTCGGGGGACGTGCCTCCTTAAGTTTAAGTAA SVRHQHLLLCVRGTCLLKFK* -1.877 1.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23421 SSTVVILYPLVQIFRRYGRY 20 SLAY-screened peptide P1771 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTCTACTGTGGTGATCCTTTATCCCCTGGTCCAGATCTTTCGTCGCTACGGCCGCTACTAA SSTVVILYPLVQIFRRYGRY* -1.877 0.006572 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23422 MPVTFLGINTCAYPPRRDYK 20 SLAY-screened peptide P1772 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCCCGTTACTTTTCTGGGTATCAATACTTGCGCGTATCCCCCTAGGAGGGACTACAAGTAA MPVTFLGINTCAYPPRRDYK* -1.876 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23423 TVCIPSASSTQFASMRPPWI 20 SLAY-screened peptide P1773 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTGTGCATCCCCTCTGCGAGCAGCACCCAGTTTGCGAGCATGCGCCCTCCCTGGATTTAA TVCIPSASSTQFASMRPPWI* -1.876 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23424 DTPTVIISNPHRHTSFGAGY 20 SLAY-screened peptide P1774 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACACGCCGACCGTCATTATTTCTAACCCTCACAGGCATACCTCCTTTGGCGCTGGCTACTAA DTPTVIISNPHRHTSFGAGY* -1.875 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23425 PRIPIRSRRSNSTTI 15 SLAY-screened peptide P1775 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCGCATTCCGATTCGGTCTCGTCGCTCTAATAGCACTACGATTTAGTGCAGTTTCGATTAA PRIPIRSRRSNSTTI*CSFD* -1.874 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23426 LRFKPLHLDFAFHSSLPMVS 20 SLAY-screened peptide P1776 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCGTTTTAAGCCTCTTCATCTTGATTTCGCCTTCCATTCCTCCTTGCCGATGGTGAGCTAA LRFKPLHLDFAFHSSLPMVS* -1.874 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23427 YNSTAKLDLCGVTNHTRIRW 20 SLAY-screened peptide P1777 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAACTCGACTGCGAAGCTTGACCTTTGTGGCGTCACCAACCATACTCGCATCAGGTGGTAA YNSTAKLDLCGVTNHTRIRW* -1.874 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23428 YKRIHIAISFHGWSVHFELH 20 SLAY-screened peptide P1778 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAAGCGCATTCATATCGCGATTAGTTTTCATGGTTGGTCCGTTCACTTTGAGCTTCACTAA YKRIHIAISFHGWSVHFELH* -1.874 0.014519 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23429 DWPHFRIGLFISPQVYTHLS 20 SLAY-screened peptide P1779 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTGGCCTCACTTTCGCATTGGTCTTTTTATCAGCCCGCAGGTTTACACTCATCTTTCTTAA DWPHFRIGLFISPQVYTHLS* -1.872 0.016207 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23430 PKGLGMSLARSIDYWCGFLI 20 SLAY-screened peptide P1780 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAAGGGCCTCGGCATGAGTCTCGCTCGGAGTATCGACTACTGGTGTGGTTTTCTGATCTAA PKGLGMSLARSIDYWCGFLI* -1.871 9.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23431 DAVPTAHSSRKYLPQDSPTT 20 SLAY-screened peptide P1781 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGCTGTCCCTACTGCCCACAGCAGTAGGAAGTACTTGCCCCAGGACTCCCCGACCACTTAA DAVPTAHSSRKYLPQDSPTT* -1.87 8.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23432 FWLYLSCICILNHSHN 16 SLAY-screened peptide P1782 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTGGCTCTACCTGAGCTGCATTTGCATCCTTAATCACAGTCATAATTAGCACTGTAACTAA FWLYLSCICILNHSHN*HCN* -1.87 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23433 LHADAIHIYELGYHPLLYVS 20 SLAY-screened peptide P1783 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACGCCGATGCTATTCATATCTACGAGCTTGGTTATCACCCGCTGCTCTACGTTTCCTAA LHADAIHIYELGYHPLLYVS* -1.87 0.001007 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23434 GTPKPPFRSNVGSYETALYH 20 SLAY-screened peptide P1784 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTACGCCGAAGCCCCCGTTCCGTTCTAACGTGGGTTCGTATGAGACCGCGCTCTACCATTAA GTPKPPFRSNVGSYETALYH* -1.87 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23435 GAPSVNCTNR 10 SLAY-screened peptide P1785 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGCTCCCTCGGTTAACTGCACGAATCGTTAGACGAACGGCCGCGGTAATTGCATCTCTTAA GAPSVNCTNR*TNGRGNCIS* -1.87 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23436 HTHFNCSVLARVRTVPTVAIN 21 SLAY-screened peptide P1786 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACACGCATTTCAATTGTTCCGTCTTGGCTAGGGTTAGGACTGTTCCTACAGTCGCTATTAAC HTHFNCSVLARVRTVPTVAIN -1.87 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23437 FLSHI 5 SLAY-screened peptide P1787 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCTCTCCCACATTTAGCTCCGTAATATGTTGCGTTTTGATCAGATTGCTTGGCTTCCTTAA FLSHI*LRNMLRFDQIAWLP* -1.87 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23438 RPIPMYFCRHYNLAWTQATF 20 SLAY-screened peptide P1788 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCCCATTCCTATGTACTTTTGTCGCCACTACAATCTTGCGTGGACTCAGGCGACGTTTTAA RPIPMYFCRHYNLAWTQATF* -1.869 0.006711 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23439 IRNGSANSVMSTYYPPSLIT 20 SLAY-screened peptide P1789 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAGGAACGGGTCCGCTAATTCCGTTATGAGCACTTACTATCCGCCTTCCCTCATTACCTAA IRNGSANSVMSTYYPPSLIT* -1.868 5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23440 AQVLVTMVSTALAP 14 SLAY-screened peptide P1790 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCAGGTCTTGGTCACTATGGTGAGCACTGCCCTTGCTCCTTAGGGGGCTAGCCACCCCTAA AQVLVTMVSTALAP*GASHP* -1.867 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23441 SSPVLYTSFTIGLWTYRHCE 20 SLAY-screened peptide P1791 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGAGTCCCGTTTTGTATACTAGCTTTACTATTGGGCTGTGGACTTACCGCCATTGTGAGTAA SSPVLYTSFTIGLWTYRHCE* -1.865 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23442 NTCISPVTNNYTLAHVFLHN 20 SLAY-screened peptide P1792 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACACGTGTATTAGTCCCGTTACTAATAACTATACCCTTGCCCACGTTTTCTTGCATAATTAA NTCISPVTNNYTLAHVFLHN* -1.865 0.005656 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23443 KHWNTECCCSGNGN 14 SLAY-screened peptide P1793 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCATTGGAATACCGAGTGCTGTTGTAGTGGCAACGGTAATTAGTCGCTTCACATGCTTTAC KHWNTECCCSGNGN*SLHMLY -1.865 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23444 RCRRLIAFYKYWRMRSIWLRN 21 SLAY-screened peptide P1794 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTGCCGGCGCCTTATCGCGTTTTACAAGTACTGGCGCATGCGTTCAATTTGGCTGCGTAAC RCRRLIAFYKYWRMRSIWLRN -1.865 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23445 PGNCRPAGFNRIRDSPSYAF 20 SLAY-screened peptide P1795 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGCAACTGTCGGCCTGCGGGCTTTAATCGTATTCGGGATTCCCCGAGTTATGCGTTCTAA PGNCRPAGFNRIRDSPSYAF* -1.865 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23446 HPTIP 5 SLAY-screened peptide P1796 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCGACTATCCCCTAGTACTACAATGATACGCATCGTCTTATTGCTTCGACTCACTATTAA HPTIP*YYNDTHRLIASTHY* -1.865 6.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23447 FPTAPHGGDLYRDLPALSCT 20 SLAY-screened peptide P1797 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCCTACTGCTCCTCACGGTGGTGACCTCTACCGTGATCTGCCTGCGCTTAGTTGCACCTAA FPTAPHGGDLYRDLPALSCT* -1.865 0.000171 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23448 LNHMHNLLHLLTRLARSFRRN 21 SLAY-screened peptide P1798 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAATCATATGCACAACCTCCTTCATTTGCTAACACGGCTAGCAAGGTCATTCCGTCGTAAC LNHMHNLLHLLTRLARSFRRN -1.864 0.000163 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23449 SSTVFAKPGRLIPSFSFFGC 20 SLAY-screened peptide P1799 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAGCACCGTTTTTGCGAAGCCTGGGCGTTTGATCCCCAGCTTCTCCTTTTTCGGTTGCTAA SSTVFAKPGRLIPSFSFFGC* -1.864 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23450 TCS 3 SLAY-screened peptide P1800 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGCTCTTAGCTGCCCGGGCTGCACACTAACCCTATTTTTACCATGCGGTGTCGGTAGTAA TCS*LPGLHTNPIFTMRCR** -1.864 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23451 PMCVKRLLTLLTRRFRLAVSN 21 SLAY-screened peptide P1801 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCATGTGTGTGAAGCGTCTGTTGACTCTGTTGACCCGTCGTTTTAGGCTCGCTGTGAGTAAC PMCVKRLLTLLTRRFRLAVSN -1.863 0.000212 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23452 PPLFQPSQAVQYKGSPDSGS 20 SLAY-screened peptide P1802 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTCTGTTTCAGCCTTCCCAGGCCGTTCAGTACAAGGGGTCCCCTGATTCGGGCAGCTAA PPLFQPSQAVQYKGSPDSGS* -1.863 0.007613 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23453 APVLNCYMLSIFRNVR 16 SLAY-screened peptide P1803 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCGGTTTTGAATTGCTATATGCTGAGTATTTTTCGGAACGTCCGTTAGACCACTATTTAA APVLNCYMLSIFRNVR*TTI* -1.862 0.010706 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23454 ALFMQYATHRRQLSPGRFVH 20 SLAY-screened peptide P1804 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCTGTTTATGCAGTACGCCACCCATCGGCGTCAGCTTAGTCCGGGGCGTTTCGTCCACTAA ALFMQYATHRRQLSPGRFVH* -1.862 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23455 AAPLLHPWGTPLYDRVLITY 20 SLAY-screened peptide P1805 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGCCCCTCTTCTGCATCCTTGGGGTACTCCGCTTTACGATAGGGTTTTGATCACTTACTAA AAPLLHPWGTPLYDRVLITY* -1.862 0.001199 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23456 SINPISDQLEVHPNCHMVHH 20 SLAY-screened peptide P1806 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTATTAATCCTATCAGTGACCAGCTTGAGGTCCATCCGAATTGCCATATGGTCCACCATTAA SINPISDQLEVHPNCHMVHH* -1.862 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23457 RQPSIGLSHDYNPPFHRSSQ 20 SLAY-screened peptide P1807 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCAGCCCTCTATTGGCCTGTCTCATGACTATAACCCCCCTTTCCATAGGAGTTCTCAGTAA RQPSIGLSHDYNPPFHRSSQ* -1.861 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23458 RYTNYSTCNLE 11 SLAY-screened peptide P1808 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTATACTAATTATTCTACTTGTAACCTTGAGTAGCGTAGCATCATTATTGCGGGGATCTAA RYTNYSTCNLE*RSIIIAGI* -1.861 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23459 HSTQLNFSSVNTINILNLSN 20 SLAY-screened peptide P1809 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTCCACTCAGCTTAACTTTAGTTCTGTGAACACCATCAACATTCTTAACCTCAGTAACTAA HSTQLNFSSVNTINILNLSN* -1.86 2.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23460 LNNCKPPVLPTTSVHPFSWR 20 SLAY-screened peptide P1810 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAACAATTGCAAGCCTCCTGTCCTCCCTACTACTTCGGTGCATCCTTTTTCGTGGCGGTAA LNNCKPPVLPTTSVHPFSWR* -1.859 0.024152 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23461 RYIEGSHRYFMHPVYYLFEP 20 SLAY-screened peptide P1811 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTATATCGAGGGCTCGCATCGGTATTTTATGCACCCCGTGTATTACCTCTTCGAGCCCTAA RYIEGSHRYFMHPVYYLFEP* -1.859 0.005173 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23462 HYPRIDHNFRDF 12 SLAY-screened peptide P1812 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTACCCCCGGATCGACCATAACTTTCGTGACTTTTAGTATCATTCTAGCTGCTGTTCGTAA HYPRIDHNFRDF*YHSSCCS* -1.858 0.000267 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23463 YNGLNSPSYTEGCVYLV 17 SLAY-screened peptide P1813 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAATGGCCTCAACAGTCCCTCTTATACGGAGGGTTGCGTCTACTTGGTTTAGGTGTCCTAC YNGLNSPSYTEGCVYLV*VSY -1.858 0.000815 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23464 CRRVNAYRPIKSSGSQNSLY 20 SLAY-screened peptide P1814 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAGGCGCGTTAATGCCTACCGCCCTATTAAGTCGTCCGGTTCTCAGAACAGCCTCTACTAA CRRVNAYRPIKSSGSQNSLY* -1.858 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23465 NPWLSHFVRMLILRLFRG 18 SLAY-screened peptide P1815 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCCCTGGCTTTCGCATTTCGTTAGGATGTTAATATTAAGGCTATTCAGGGGCTGAAGTAAC NPWLSHFVRMLILRLFRG*SN -1.858 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23466 SWADIRRGQNTLQKTDD 17 SLAY-screened peptide P1816 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTGGGCTGACATCCGTCGGGGCCAGAACACTTTGCAGAAGACTGACGACTAGGCTACTTAA SWADIRRGQNTLQKTDD*AT* -1.856 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23467 VCKRRYPPDSSPPRSDQAST 20 SLAY-screened peptide P1817 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTGCAAGAGGAGGTACCCGCCTGATTCCAGCCCGCCTCGGAGTGATCAGGCTAGTACCTAA VCKRRYPPDSSPPRSDQAST* -1.856 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23468 NEPKTTSVLTLFCLVCLLPSN 21 SLAY-screened peptide P1818 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGAGCCGAAGACGACCAGCGTATTAACACTATTTTGTCTAGTATGTCTACTCCCTAGTAAC NEPKTTSVLTLFCLVCLLPSN -1.855 0.007009 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23469 CLFPCNTHCYYFLRVLPIDT 20 SLAY-screened peptide P1819 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTCTTTCCTTGCAACACCCATTGTTATTATTTTTTGCGGGTGCTTCCCATTGACACGTAA CLFPCNTHCYYFLRVLPIDT* -1.855 0.001135 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23470 HDVAHLLRCGSNPGAWAQTS 20 SLAY-screened peptide P1820 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGATGTCGCCCATCTGCTTCGCTGCGGTAGTAACCCTGGCGCGTGGGCCCAGACTTCTTAA HDVAHLLRCGSNPGAWAQTS* -1.855 0.000156 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23471 GRHIIFLSVVMLRFSRRDSV 20 SLAY-screened peptide P1821 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCGTCATATTATTTTTTTGAGCGTCGTGATGTTGAGGTTCAGCAGGCGGGATAGTGTCTAA GRHIIFLSVVMLRFSRRDSV* -1.855 0.005455 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23472 SANYVVHPAINGGTPPWSLI 20 SLAY-screened peptide P1822 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGCTAATTACGTGGTCCACCCGGCCATCAACGGGGGGACGCCGCCCTGGTCGCTTATCTAA SANYVVHPAINGGTPPWSLI* -1.855 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23473 NSYAYPYRFYLLSLTSANRA 20 SLAY-screened peptide P1823 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTCCTATGCCTATCCCTATCGTTTCTACCTCCTTTCCCTCACGAGTGCCAATCGTGCCTAA NSYAYPYRFYLLSLTSANRA* -1.854 0.008738 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23474 TSLVAAAVDARRARMHESLP 20 SLAY-screened peptide P1824 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCAGCCTGGTTGCTGCTGCTGTTGACGCTAGGCGGGCCAGGATGCACGAGTCCCTGCCTTAA TSLVAAAVDARRARMHESLP* -1.854 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23475 PVAHVPKLDLNCFLAPNCYS 20 SLAY-screened peptide P1825 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGTTGCTCATGTTCCGAAGCTCGATCTCAACTGCTTTTTGGCCCCTAATTGTTACAGTTAA PVAHVPKLDLNCFLAPNCYS* -1.853 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23476 NPRKLATSKNCHIPQILDF 19 SLAY-screened peptide P1826 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCCGCGCAAGCTTGCGACGTCCAAGAATTGCCACATCCCGCAGATCCTCGATTTTTAGTAA NPRKLATSKNCHIPQILDF** -1.853 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23477 NLHPDGKTLTHVLSRALHPP 20 SLAY-screened peptide P1827 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCTCCACCCTGACGGCAAGACCTTGACCCATGTGCTCTCCAGGGCGTTGCATCCTCCGTAA NLHPDGKTLTHVLSRALHPP* -1.853 0.006547 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23478 ADENLSARGVFRCLHY 16 SLAY-screened peptide P1828 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGATGAGAACCTGTCTGCTCGAGGCGTCTTTCGCTGCCTACATTACTAGTTACTAACTGAG ADENLSARGVFRCLHY*LLTE -1.852 9.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23479 PA 2 SLAY-screened peptide P1829 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCGTAGTCGGTCATCCATGCCACTGAGGCGCACGTGCAGAAGTTTTTTGATCTTAACTAA PA*SVIHATEAHVQKFFDLN* -1.852 0.000133 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23480 RNTNVHMLEPWLTDSLILLGN 21 SLAY-screened peptide P1830 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAATACCAATGTGCATATGTTGGAGCCTTGGCTTACCGATAGCCTCATACTACTGGGTAAC RNTNVHMLEPWLTDSLILLGN -1.852 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23481 CSSPFSPQVF 10 SLAY-screened peptide P1831 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTCGAGTCCCTTTTCGCCGCAGGTTTTTTAGCCCTATGGCCTTTAGGGGATTACGTGCTAA CSSPFSPQVF*PYGL*GITC* -1.85 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23482 HRPTPQPRCNVIHSRQNNDL 20 SLAY-screened peptide P1832 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGTCCGACGCCTCAGCCTCGTTGTAACGTTATCCATTCCAGGCAGAATAACGACCTCTAA HRPTPQPRCNVIHSRQNNDL* -1.85 0.043603 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23483 SMPTSYPNSYDVA 13 SLAY-screened peptide P1833 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTATGCCTACTAGCTATCCTAACTCTTATGATGTCGCCTAGATTTCGGTGGCGAAGCGGTAA SMPTSYPNSYDVA*ISVAKR* -1.85 0.000227 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23484 GPAYLYISYWTLNYNPYIYK 20 SLAY-screened peptide P1834 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCCGGCTTACCTGTACATTAGCTATTGGACCTTGAATTACAACCCCTATATTTATAAGTAA GPAYLYISYWTLNYNPYIYK* -1.849 0.013233 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23485 PPPGGTPSNPYEAGVTRPHF 20 SLAY-screened peptide P1835 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCGCCTGGCGGGACCCCGTCGAATCCTTACGAGGCCGGCGTGACCAGGCCCCATTTCTAA PPPGGTPSNPYEAGVTRPHF* -1.849 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23486 THYRWLLFPQF 11 SLAY-screened peptide P1836 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCATTATCGTTGGCTCTTGTTCCCACAATTCTAGCTCGTGTTATTGTATCTAACTGAGTAA THYRWLLFPQF*LVLLYLTE* -1.848 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23487 LAPHLYATRCVLSVLLIQHN 20 SLAY-screened peptide P1837 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGCTCCGCATCTTTATGCGACCCGTTGCGTTCTTTCCGTGCTGTTGATTCAGCATAACTAA LAPHLYATRCVLSVLLIQHN* -1.848 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23488 TNYGRTFIGADIFI 14 SLAY-screened peptide P1838 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAATTATGGTCGCACCTTTATCGGGGCGGATATCTTCATCTGAGTAACGATGGCGGTTAAC TNYGRTFIGADIFI*VTMAVN -1.848 0.002841 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23489 YDAPRTFKVDC 11 SLAY-screened peptide P1839 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGACGCTCCTCGCACTTTCAAGGTCGATTGCTAGACGAGTCACAACCAGAGCCCCTTCTAA YDAPRTFKVDC*TSHNQSPF* -1.848 0.045375 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23490 LPCPPSWATSMNCPFVASQA 20 SLAY-screened peptide P1840 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCGTGCCCCCCTAGCTGGGCTACCTCCATGAATTGCCCGTTTGTGGCTAGCCAGGCCTAA LPCPPSWATSMNCPFVASQA* -1.845 0.000509 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23491 HQRSDKCVATSDAYLSSKPT 20 SLAY-screened peptide P1841 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCAGCGGTCCGACAAGTGTGTTGCCACTTCGGATGCTTATCTTAGCTCCAAGCCTACCTAA HQRSDKCVATSDAYLSSKPT* -1.845 0.002404 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23492 HVFEPFDNLPMNPIWSNQ 18 SLAY-screened peptide P1842 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGTTTTCGAGCCCTTCGACAATCTTCCGATGAATCCTATTTGGTCTAACCAGTAGAGCTAA HVFEPFDNLPMNPIWSNQ*S* -1.845 0.000601 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23493 LNFSAVRQDGTHRTSSNSNH 20 SLAY-screened peptide P1843 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGAATTTCTCCGCTGTCCGGCAGGATGGCACTCACCGTACTTCGAGTAACTCTAATCATTAA LNFSAVRQDGTHRTSSNSNH* -1.844 0.000216 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23494 THHKNWMSSYPTVQNLIYYT 20 SLAY-screened peptide P1844 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCATCACAAGAATTGGATGTCCTCTTATCCGACTGTTCAGAATCTGATTTACTACACCTAA THHKNWMSSYPTVQNLIYYT* -1.844 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23495 SALAVVTSRTSCTYTPN 17 SLAY-screened peptide P1845 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGCGCTGGCCGTCGTTACTTCCCGTACTTCTTGCACTTATACTCCGAACTAGTCTCTGTAA SALAVVTSRTSCTYTPN*SL* -1.844 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23496 KTITLLLVAYNSNNIS 16 SLAY-screened peptide P1846 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGACTATTACCCTCCTTTTGGTTGCTTACAATTCGAACAATATTTCCTAGGTCACGAGGTAA KTITLLLVAYNSNNIS*VTR* -1.844 0.00062 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23497 NILCTGSSRRFMLIIARTTCN 21 SLAY-screened peptide P1847 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACATCCTTTGTACTGGTAGTTCGCGTCGCTTCATGCTTATTATTGCGCGTACTACATGTAAC NILCTGSSRRFMLIIARTTCN -1.843 0.001629 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23498 RY 2 SLAY-screened peptide P1848 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTATTAGGTGCGTAGGCCCAACCACATGGGTCATTACGATGTTTTTGATTTCATGAACTAA RY*VRRPNHMGHYDVFDFMN* -1.843 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23499 SYPDQVRPHNPHGILSVDNS 20 SLAY-screened peptide P1849 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTACCCCGACCAGGTTAGGCCGCACAATCCTCATGGTATTCTCAGTGTTGATAACAGCTAA SYPDQVRPHNPHGILSVDNS* -1.843 0.000118 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23500 TYHIAISSSNALLTAALPRG 20 SLAY-screened peptide P1850 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTATCATATTGCTATCTCCTCTAGTAATGCCCTGCTTACCGCTGCGTTGCCTCGTGGTTAA TYHIAISSSNALLTAALPRG* -1.843 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23501 NRVAEVKDNDNGLVTATVAFN 21 SLAY-screened peptide P1851 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCGGGTTGCTGAGGTTAAGGATAATGACAACGGCCTGGTTACTGCGACCGTGGCTTTTAAC NRVAEVKDNDNGLVTATVAFN -1.842 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23502 GRSHLATFDPHDWHIRLPILN 21 SLAY-screened peptide P1852 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCGCTCTCATCTGGCTACGTTTGACCCCCACGATTGGCATATTAGGTTGCCGATACTTAAC GRSHLATFDPHDWHIRLPILN -1.841 0.000608 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23503 PHSSFDAADYYTGQSCVKCY 20 SLAY-screened peptide P1853 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCATTCGTCCTTCGACGCCGCGGACTACTATACTGGTCAGTCGTGCGTGAAGTGTTATTAA PHSSFDAADYYTGQSCVKCY* -1.841 0.001893 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23504 YTPFHSFPIWPCPPSLHLAV 20 SLAY-screened peptide P1854 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACGCCCTTTCATTCGTTTCCTATCTGGCCTTGTCCTCCCAGCCTGCACCTCGCGGTTTAA YTPFHSFPIWPCPPSLHLAV* -1.841 0.013543 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23505 SHGLGDKPSPHNNSNIVCPN 20 SLAY-screened peptide P1855 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCATGGTCTCGGTGACAAGCCGTCGCCGCACAACAACTCTAATATTGTTTGCCCTAATTAA SHGLGDKPSPHNNSNIVCPN* -1.841 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23506 RYYPRNWRHLYNNVRTHLTS 20 SLAY-screened peptide P1856 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTATTATCCTCGGAACTGGCGGCACCTGTATAATAATGTCCGGACCCATCTTACTTCCTAA RYYPRNWRHLYNNVRTHLTS* -1.84 6.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23507 ILSSNQLMCCVPRSSLIHRL 20 SLAY-screened peptide P1857 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCTCTCGTCTAACCAGCTTATGTGTTGTGTCCCGCGTAGTAGTCTTATTCACAGGCTTTAA ILSSNQLMCCVPRSSLIHRL* -1.839 0.001689 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23508 RTIARLLYAFLRRVSACSGM 20 SLAY-screened peptide P1858 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGACCATCGCTCGTCTGCTCTATGCTTTCCTTCGTCGGGTTAGCGCGTGCTCCGGTATGTAA RTIARLLYAFLRRVSACSGM* -1.839 0.012215 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23509 PRYLTDFLLRPADSQYSDPH 20 SLAY-screened peptide P1859 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGCTACCTGACCGACTTCTTGCTGCGGCCTGCTGATTCCCAGTACTCCGACCCGCATTAA PRYLTDFLLRPADSQYSDPH* -1.839 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23510 FKTIHMHGVSVTPMG 15 SLAY-screened peptide P1860 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTAAGACTATTCACATGCACGGTGTATCTGTTACACCTATGGGTTGATTTATATGAAGTAAC FKTIHMHGVSVTPMG*FI*SN -1.838 0.000697 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23511 LLNPFNDRDSMPLSHFYMLL 20 SLAY-screened peptide P1861 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCTTAACCCGTTCAATGACCGCGATTCCATGCCTCTTAGTCACTTTTACATGCTGCTGTAA LLNPFNDRDSMPLSHFYMLL* -1.838 0.000171 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23512 GAR 3 SLAY-screened peptide P1862 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTGCCCGCTAGGGCATTTTTAGCTGCACCAATAATAACGAGGTCTGTTATTCTTATTGCTAA GAR*GIFSCTNNNEVCYSYC* -1.838 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23513 CFDRPHNFINGIPFLRIYTT 20 SLAY-screened peptide P1863 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTTCGATCGTCCCCACAACTTCATTAACGGTATTCCCTTTTTGCGCATTTACACTACCTAA CFDRPHNFINGIPFLRIYTT* -1.838 2.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23514 MNKHPAPGCPGHAHRSLKFW 20 SLAY-screened peptide P1864 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGAACAAGCACCCTGCTCCCGGCTGTCCCGGCCACGCGCATCGTTCTCTGAAGTTTTGGTAA MNKHPAPGCPGHAHRSLKFW* -1.837 0.000622 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23515 LVINMLCKTIQLFKLIQLVF 20 SLAY-screened peptide P1865 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGTGATTAATATGCTTTGCAAGACCATTCAGCTTTTCAAGCTCATTCAGCTTGTGTTTTAA LVINMLCKTIQLFKLIQLVF* -1.837 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23516 VSTPSPLFGKDPHWWTQALR 20 SLAY-screened peptide P1866 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTCTACCCCTAGCCCTCTGTTTGGCAAGGATCCGCATTGGTGGACCCAGGCGCTCCGTTAA VSTPSPLFGKDPHWWTQALR* -1.836 0.003219 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23517 VSSFLLSTYGGLRMHTFIPV 20 SLAY-screened peptide P1867 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTCCAGCTTTTTGCTCTCCACGTATGGTGGCCTGCGTATGCACACCTTCATCCCCGTGTAA VSSFLLSTYGGLRMHTFIPV* -1.836 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23518 FEGRQAAD 8 SLAY-screened peptide P1868 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGAGGGGCGCCAGGCTGCGGATTAGGCTCATTGCTGCAATATGCATGCCCCTGACACTTAA FEGRQAAD*AHCCNMHAPDT* -1.836 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23519 YTAPRADILKKRLNNSLDTV 20 SLAY-screened peptide P1869 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATACTGCGCCGCGGGCGGACATCCTGAAGAAGCGCTTGAACAATTCGTTGGATACCGTGTAA YTAPRADILKKRLNNSLDTV* -1.835 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23520 SRLKVPRGHPLAPYDRGMMA 20 SLAY-screened peptide P1870 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCGCCTCAAGGTCCCCCGGGGGCACCCGCTGGCGCCGTATGATCGCGGCATGATGGCGTAA SRLKVPRGHPLAPYDRGMMA* -1.834 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23521 INIPKLILILLISADHHQMP 20 SLAY-screened peptide P1871 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAACATTCCTAAGCTTATTCTCATTCTTTTGATCTCTGCGGACCACCACCAGATGCCCTAA INIPKLILILLISADHHQMP* -1.834 0.000583 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23522 NTFNTLGCFFSDPMP 15 SLAY-screened peptide P1872 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATACCTTTAATACCTTGGGCTGTTTCTTCTCTGACCCAATGCCGTGATGACTACGCTCTAAC NTFNTLGCFFSDPMP**LRSN -1.834 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23523 LCPSQHPLYFALAGTPITLLN 21 SLAY-screened peptide P1873 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTGTCCTTCCCAGCACCCTCTGTACTTCGCGTTAGCCGGCACTCCGATAACGCTCCTTAAC LCPSQHPLYFALAGTPITLLN -1.834 0.011689 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23524 VHRRPITSYNPWDCYFLHHY 20 SLAY-screened peptide P1874 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCACCGCCGTCCTATCACTTCGTATAACCCCTGGGACTGCTATTTCCTTCACCATTATTAA VHRRPITSYNPWDCYFLHHY* -1.833 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23525 GTGFFVGINISNPPRLYVAW 20 SLAY-screened peptide P1875 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTACGGGCTTTTTTGTGGGCATCAATATCTCTAATCCCCCCAGGCTTTATGTTGCGTGGTAA GTGFFVGINISNPPRLYVAW* -1.832 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23526 SVHSRLPDVYGSYSPSSGGT 20 SLAY-screened peptide P1876 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGTCCACTCCAGGCTGCCCGATGTTTATGGGAGCTACTCCCCTAGTTCTGGGGGTACGTAA SVHSRLPDVYGSYSPSSGGT* -1.831 0.011554 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23527 WIPKRPELRGCSYGNPPESF 20 SLAY-screened peptide P1877 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGATTCCCAAGCGCCCGGAGTTGAGGGGCTGTTCTTACGGGAATCCTCCCGAGAGTTTCTAA WIPKRPELRGCSYGNPPESF* -1.831 0.00787 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23528 ILNENNQPWNRCVIYYLAQR 20 SLAY-screened peptide P1878 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTTGAACGAGAATAACCAGCCGTGGAACCGCTGCGTCATTTATTACCTGGCCCAGCGTTAA ILNENNQPWNRCVIYYLAQR* -1.831 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23529 RIQPVHDR 8 SLAY-screened peptide P1879 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCATTCAGCCTGTTCATGATCGCTAGTGGATTAATTACGGCCAGAGCATGCTTGATTCCTAA RIQPVHDR*WINYGQSMLDS* -1.831 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23530 YFPLCFRVNRSASSHRRFRR 20 SLAY-screened peptide P1880 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTTTCCTCTCTGCTTCCGTGTCAATCGTTCGGCTAGTTCCCATCGTCGTTTTCGTAGGTAA YFPLCFRVNRSASSHRRFRR* -1.831 0.030331 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23531 ISVVPTYRNKFDCTLCHHKY 20 SLAY-screened peptide P1881 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTTCCGTGGTGCCTACGTATAGGAACAAGTTCGACTGCACTTTGTGTCATCACAAGTACTAA ISVVPTYRNKFDCTLCHHKY* -1.831 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23532 SCGHHFGTTNKANPRCYVVC 20 SLAY-screened peptide P1882 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTGTGGGCATCACTTTGGTACCACGAATAAGGCTAATCCGAGGTGTTATGTTGTCTGTTAA SCGHHFGTTNKANPRCYVVC* -1.83 0.000521 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23533 RHTYPSPISRVGSPRARTWI 20 SLAY-screened peptide P1883 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCACACTTACCCTAGTCCTATCTCTAGGGTGGGCTCCCCCCGTGCTCGGACTTGGATTTAA RHTYPSPISRVGSPRARTWI* -1.83 0.011328 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23534 WQIPPGCRIARA 12 SLAY-screened peptide P1884 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCAGATTCCTCCCGGTTGCCGTATCGCTCGGGCTTAGGTTTTCTCCCTTATCGAGCCCTAA WQIPPGCRIARA*VFSLIEP* -1.83 0.014309 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23535 YKSSKHRITQPNSTVQTCLR 20 SLAY-screened peptide P1885 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAAGTCCAGCAAGCACAGGATTACTCAGCCCAACTCGACTGTTCAGACTTGCTTGAGGTAA YKSSKHRITQPNSTVQTCLR* -1.829 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23536 LPLHGVADNHRDIARAVPI 19 SLAY-screened peptide P1886 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCTCTCCACGGGGTTGCGGACAACCACAGGGATATCGCTCGTGCCGTCCCTATCTAGTAA LPLHGVADNHRDIARAVPI** -1.829 5.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23537 SIIDSEPTDRYYDRFYYYIF 20 SLAY-screened peptide P1887 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCATTATTGACTCCGAGCCTACTGATCGCTATTATGACCGCTTTTACTACTATATCTTCTAA SIIDSEPTDRYYDRFYYYIF* -1.828 0.000225 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23538 PNRAVRGFANISTTIPIGR 19 SLAY-screened peptide P1888 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAACCGGGCTGTGAGGGGCTTTGCCAATATTTCTACGACTATTCCGATTGGCCGCTAGTAA PNRAVRGFANISTTIPIGR** -1.828 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23539 RTPRPPRVPTIDPYFLMDMY 20 SLAY-screened peptide P1889 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACCCCGCGGCCCCCCCGTGTGCCTACTATTGATCCGTATTTTCTTATGGATATGTATTAA RTPRPPRVPTIDPYFLMDMY* -1.828 0.007071 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23540 RLPYKFFSGIIRCAVWIIYP 20 SLAY-screened peptide P1890 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTGCCCTATAAGTTCTTCTCGGGTATTATTCGCTGTGCCGTGTGGATTATTTATCCCTAA RLPYKFFSGIIRCAVWIIYP* -1.828 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23541 PLVCSRCAVTVMSCL 15 SLAY-screened peptide P1891 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTGTCTGCTCGCGTTGCGCCGTGACCGTAATGTCGTGTTTGTGAGCTTTACCTCTTAAC PLVCSRCAVTVMSCL*ALPLN -1.828 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23542 QTSHNTWDHFSSSIHVSDNL 20 SLAY-screened peptide P1892 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGACCTCTCACAATACCTGGGATCATTTTTCTTCGTCTATTCATGTTTCTGATAACCTTTAA QTSHNTWDHFSSSIHVSDNL* -1.827 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23543 TRASHARHWHPHLLYYQGTL 20 SLAY-screened peptide P1893 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCGCGCTTCCCATGCCCGCCACTGGCACCCCCACCTTCTGTATTACCAGGGGACTCTTTAA TRASHARHWHPHLLYYQGTL* -1.827 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23544 SLLVLMISPTVRRTGFTRSSN 21 SLAY-screened peptide P1894 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTTGTTGGTGCTGATGATTAGTCCCACGGTTCGTCGGACTGGTTTTACACGATCGTCTAAC SLLVLMISPTVRRTGFTRSSN -1.827 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23545 PPTRRSTTRATAGYDAPAHL 20 SLAY-screened peptide P1895 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCCACTAGGCGGTCCACGACGCGCGCCACCGCCGGCTACGACGCGCCGGCTCACCTCTAA PPTRRSTTRATAGYDAPAHL* -1.826 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23546 NKEPVDHSTEGNQNLDYSSC 20 SLAY-screened peptide P1896 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACAAGGAGCCGGTCGACCACAGTACGGAGGGCAATCAGAATCTGGACTATAGTTCGTGCTAA NKEPVDHSTEGNQNLDYSSC* -1.826 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23547 GEAPDVADSICSLHWGL 17 SLAY-screened peptide P1897 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCGAGGCTCCCGATGTTGCTGATTCTATCTGTTCTTTGCACTGGGGGCTTTAGACCATTTAA GEAPDVADSICSLHWGL*TI* -1.825 0.004574 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23548 ATRDDSE 7 SLAY-screened peptide P1898 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTACTCGCGATGACTCGGAGTAGCCAGCCGGACTCCTACCTCGAAGTATCAGTGTTTTTAAC ATRDDSE*PAGLLPRSISVFN -1.824 0.019832 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23549 RALSLLCTLRRLFLPSI 17 SLAY-screened peptide P1899 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGCCCTCAGCCTTCTTTGTACCCTTAGGCGTTTGTTTCTTCCCTCTATTTAGAAGATGTAA RALSLLCTLRRLFLPSI*KM* -1.824 0.002262 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23550 YASWRSAAYFDIMGVFYSLP 20 SLAY-screened peptide P1900 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCTTCGTGGCGGTCCGCTGCTTACTTCGACATCATGGGCGTCTTTTACAGTCTCCCCTAA YASWRSAAYFDIMGVFYSLP* -1.823 0.001628 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23551 LWGVSCSPRGNPCRCLGLDA 20 SLAY-screened peptide P1901 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTGGGGCGTCTCTTGCAGCCCGCGTGGTAACCCGTGCCGTTGTCTTGGTTTGGACGCTTAA LWGVSCSPRGNPCRCLGLDA* -1.823 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23552 RAHLITLSFVLLASEEQYDR 20 SLAY-screened peptide P1902 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGGCCCACCTTATCACCTTGTCCTTTGTTCTCCTTGCCAGCGAGGAGCAGTATGACCGGTAA RAHLITLSFVLLASEEQYDR* -1.823 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23553 ARGWMPYGHYMSVAQTANYM 20 SLAY-screened peptide P1903 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCGCGGCTGGATGCCTTATGGTCACTACATGTCCGTGGCGCAGACCGCGAATTACATGTAA ARGWMPYGHYMSVAQTANYM* -1.823 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23554 PPFILVVCHYL 11 SLAY-screened peptide P1904 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCTTTATTCTGGTTGTTTGCCATTACTTGTAGCCCGCCACCTGGATCAGCTCGCGCTAA PPFILVVCHYL*PATWISSR* -1.823 0.012081 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23555 LDSFPGHSTYPRHPLHCVLL 20 SLAY-screened peptide P1905 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGACAGCTTCCCTGGTCATTCCACGTATCCCAGGCACCCCCTCCACTGTGTCCTCCTTTAA LDSFPGHSTYPRHPLHCVLL* -1.822 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23556 STSALHIIAFGSPPRAFSRGN 21 SLAY-screened peptide P1906 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTACGTCCGCTCTCCATATCATTGCATTTGGATCTCCTCCGCGAGCTTTTTCCCGCGGTAAC STSALHIIAFGSPPRAFSRGN -1.822 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23557 SSTNHFIYYIGLLHLH 16 SLAY-screened peptide P1907 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTCTACTAACCACTTTATCTACTATATCGGCTTGCTCCACCTCCACTAGCTCGATCGTTAA SSTNHFIYYIGLLHLH*LDR* -1.821 0.006986 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23558 LEDDNPQALLYA 12 SLAY-screened peptide P1908 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGAGGACGATAATCCGCAGGCGCTGCTGTACGCCTAGTACCTTCACTGTTTCTGGTACTAA LEDDNPQALLYA*YLHCFWY* -1.821 0.000176 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23559 APWNAYHLSDFRTQSHRLST 20 SLAY-screened peptide P1909 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCGTGGAACGCTTACCACCTCTCCGATTTTCGCACTCAGTCGCATCGCCTGTCCACGTAA APWNAYHLSDFRTQSHRLST* -1.82 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23560 FTMSSDNSNSKFNRSYFHQR 20 SLAY-screened peptide P1910 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCACGATGTCTAGTGATAACTCCAACAGTAAGTTTAATCGTAGCTATTTCCATCAGAGGTAA FTMSSDNSNSKFNRSYFHQR* -1.82 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23561 PL 2 SLAY-screened peptide P1911 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTGTAGGCTAATGCGCGTCCCGATGTGGATGACTCGTCCAGTCAGATCGATGGGGACTAA PL*ANARPDVDDSSSQIDGD* -1.82 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23562 GFQCYCPLNIHVI 13 SLAY-screened peptide P1912 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTTTCAGTGCTACTGTCCCCTGAACATCCACGTTATTTAGCCTGTTGGTTTGTTTCATTAA GFQCYCPLNIHVI*PVGLFH* -1.819 0.007971 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23563 RML 3 SLAY-screened peptide P1913 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCATGCTTTAGTCGGACTACATCATTAAGCGGGCCACGTCGAATATGCAGAACAATTTCTAA RML*SDYIIKRATSNMQNNF* -1.819 0.00157 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23564 PGATDPWRLVSHRFRHSGRP 20 SLAY-screened peptide P1914 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGGCGCCACCGATCCTTGGCGTCTGGTGTCCCATCGCTTCCGTCACAGTGGTCGGCCGTAA PGATDPWRLVSHRFRHSGRP* -1.819 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23565 PRGPECPIVTSLLVRVTYSS 20 SLAY-screened peptide P1915 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCGGGGCCCTGAGTGTCCGATTGTTACTTCTCTCCTTGTTAGGGTCACTTATTCCTCTTAA PRGPECPIVTSLLVRVTYSS* -1.819 0.009443 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23566 VLFYLRLGRTIVVTYNLSNT 20 SLAY-screened peptide P1916 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCTTTTCTACTTGCGCCTGGGCCGGACTATCGTTGTCACGTATAATCTCTCTAATACCTAA VLFYLRLGRTIVVTYNLSNT* -1.819 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23567 MTHTPVYSDVLHLPPSLSCVT 21 SLAY-screened peptide P1917 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGACCCACACCCCAGTTTATTCAGACGTACTTCATTTACCACCATCCTTGTCGTGCGTAACT MTHTPVYSDVLHLPPSLSCVT -1.819 8.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23568 STNFPERTVWHHMSSASTNS 20 SLAY-screened peptide P1918 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGACTAACTTCCCCGAGCGGACCGTCTGGCATCACATGTCTTCTGCTAGTACCAACAGCTAA STNFPERTVWHHMSSASTNS* -1.817 0.015958 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23569 LIQTEPQSTSLAYSATFGSY 20 SLAY-screened peptide P1919 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCATTCAGACTGAGCCCCAGTCGACCAGCCTGGCCTATAGTGCGACCTTTGGGTCCTATTAA LIQTEPQSTSLAYSATFGSY* -1.817 0.003589 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23570 MSACSSPVNFYRQTCNSYKA 20 SLAY-screened peptide P1920 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTCCGCGTGCTCTTCTCCTGTCAATTTTTATCGCCAGACTTGTAATAGTTACAAGGCTTAA MSACSSPVNFYRQTCNSYKA* -1.817 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23571 RRNSFLRCLTGRVGSTHVNP 20 SLAY-screened peptide P1921 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCGGAACTCTTTCCTCAGGTGTCTTACGGGTCGCGTTGGCTCGACTCACGTCAATCCGTAA RRNSFLRCLTGRVGSTHVNP* -1.816 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23572 PSHAPPTLPATTV 13 SLAY-screened peptide P1922 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCCCATGCCCCTCCGACGCTCCCTGCGACCACTGTTTAGGACACTCCGCACTCGCGCTAA PSHAPPTLPATTV*DTPHSR* -1.816 0.00051 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23573 DGYYFWHIDC 10 SLAY-screened peptide P1923 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATGGCTATTATTTCTGGCACATCGATTGTTAGACGCAGGCTATGTATACTGAGGGCAACTAA DGYYFWHIDC*TQAMYTEGN* -1.815 0.00034 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23574 ESTCYLPSCHPSPAVRWGSA 20 SLAY-screened peptide P1924 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGAGCACCTGCTACCTCCCCAGCTGTCACCCGTCTCCGGCGGTCAGGTGGGGTTCTGCCTAA ESTCYLPSCHPSPAVRWGSA* -1.815 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23575 SGIS 4 SLAY-screened peptide P1925 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGGTATTTCGTAGGCTCGCAGCCCGATGGCGGTTACTCTTGTTTCGTACCCGCTTACTTAA SGIS*ARSPMAVTLVSYPLT* -1.815 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23576 YVRTNAPQLAIFSAYPAHVF 20 SLAY-screened peptide P1926 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGTGCGCACTAACGCCCCCCAGTTGGCTATCTTCAGCGCTTATCCTGCGCATGTGTTCTAA YVRTNAPQLAIFSAYPAHVF* -1.815 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23577 PPNTATCLPTPFPYAKLRYT 20 SLAY-screened peptide P1927 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCGAACACTGCCACGTGCCTTCCTACTCCTTTTCCTTATGCGAAGCTTCGTTATACGTAA PPNTATCLPTPFPYAKLRYT* -1.815 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23578 HANYASCFARGETSSPCNHN 20 SLAY-screened peptide P1928 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGCTAATTATGCTTCTTGTTTTGCCAGGGGCGAGACGTCGAGTCCGTGTAACCACAATTAA HANYASCFARGETSSPCNHN* -1.815 0.0122 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23579 VCKWITTWKQILSPNKHLRP 20 SLAY-screened peptide P1929 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGTGCAAGTGGATTACTACCTGGAAGCAGATTCTCTCCCCGAATAAGCATTTGCGTCCCTAA VCKWITTWKQILSPNKHLRP* -1.815 0.013632 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23580 PPTHYCPNWYRIFRQPLLVL 20 SLAY-screened peptide P1930 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTACTCATTATTGTCCTAACTGGTACCGCATCTTTCGTCAGCCCTTGCTCGTTCTGTAA PPTHYCPNWYRIFRQPLLVL* -1.814 0.001752 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23581 SSSTMQCYELMFQ 13 SLAY-screened peptide P1931 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCTTCTACCATGCAGTGCTACGAGCTCATGTTCCAGTAGACCCGTATTTGGGTCATTTAA SSSTMQCYELMFQ*TRIWVI* -1.814 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23582 PSPFPNEPSDSPPFCYLLVA 20 SLAY-screened peptide P1932 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGCCCCTTCCCCAACGAGCCGTCCGATTCGCCGCCGTTCTGCTATCTCCTGGTTGCGTAA PSPFPNEPSDSPPFCYLLVA* -1.814 0.002075 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23583 QSVPKRRSNPTIQDLSRNCS 20 SLAY-screened peptide P1933 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTCCGTCCCGAAGAGGCGCAGTAATCCTACCATTCAGGATCTGTCGCGCAACTGCTCGTAA QSVPKRRSNPTIQDLSRNCS* -1.814 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23584 PAHTPNLLTDSFTHTRMNIT 20 SLAY-screened peptide P1934 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCCCATACCCCTAATCTTCTCACCGATTCGTTTACCCATACGAGGATGAATATTACCTAA PAHTPNLLTDSFTHTRMNIT* -1.814 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23585 IR 2 SLAY-screened peptide P1935 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCGGTAGCGCTAGTACGAGATCCCGGGGCGTTACATTAATATCAGCAGGCTTTTTAATTAA IR*R*YEIPGRYINISRLFN* -1.813 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23586 PVTMDSPTPDQRVTAHFALT 20 SLAY-screened peptide P1936 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGTTACCATGGATTCCCCTACGCCGGATCAGCGCGTTACTGCGCATTTTGCTCTCACCTAA PVTMDSPTPDQRVTAHFALT* -1.813 0.016429 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23587 CTNLGVRHLTDTMGRRVISFN 21 SLAY-screened peptide P1937 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCACGAATCTTGGCGTTCGTCATCTTACTGACACTATGGGCAGGAGAGTGATATCGTTTAAC CTNLGVRHLTDTMGRRVISFN -1.813 0.007014 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23588 YTSGFCLPIHEMA 13 SLAY-screened peptide P1938 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACGTCGGGCTTCTGCCTCCCGATCCATGAGATGGCCTAGCCCCCCTACCATAAGGAGTAA YTSGFCLPIHEMA*PPYHKE* -1.812 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23589 PSTCSFYQCEGCSNIIGLDG 20 SLAY-screened peptide P1939 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGTACCTGTAGCTTTTATCAGTGTGAGGGTTGCTCTAATATTATTGGTCTGGATGGCTAA PSTCSFYQCEGCSNIIGLDG* -1.812 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23590 ADLLPRSHYNSYMHIEEFFI 20 SLAY-screened peptide P1940 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGACTTGCTGCCTCGTTCGCACTACAACTCGTATATGCATATCGAGGAGTTTTTCATCTAA ADLLPRSHYNSYMHIEEFFI* -1.812 0.022619 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23591 AIALPSGQHGIPHVIY 16 SLAY-screened peptide P1941 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCATTGCTCTTCCCTCCGGCCAGCACGGCATTCCTCATGTCATTTATTAGAAGACCTGGTAA AIALPSGQHGIPHVIY*KTW* -1.811 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23592 YSRVIRITLPDTGFGPCHDF 20 SLAY-screened peptide P1942 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTCCCGGGTGATTAGGATTACGTTGCCGGACACTGGTTTTGGGCCTTGCCATGACTTCTAA YSRVIRITLPDTGFGPCHDF* -1.811 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23593 PHPRDDEDHFHRNNSLPA 18 SLAY-screened peptide P1943 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCACCCGCGGGATGATGAGGATCACTTCCATCGTAATAATAGCCTTCCCGCTTAACGTAAC PHPRDDEDHFHRNNSLPA*RN -1.811 0.001299 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23594 LNNRNRSLPGNPTIEYFVVE 20 SLAY-screened peptide P1944 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAATAACCGGAACCGCTCGCTGCCCGGGAACCCGACTATTGAGTATTTCGTTGTGGAGTAA LNNRNRSLPGNPTIEYFVVE* -1.811 0.00027 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23595 HSTWVAGSYYAIDRSFGSSL 20 SLAY-screened peptide P1945 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTCCACGTGGGTGGCTGGCTCGTATTACGCTATTGATAGGTCCTTTGGCTCTTCCCTCTAA HSTWVAGSYYAIDRSFGSSL* -1.809 0.000984 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23596 LVRDTTFITIRGDNTERALV 20 SLAY-screened peptide P1946 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGTCCGCGACACCACCTTTATCACGATCCGCGGTGATAATACCGAGAGGGCCTTGGTGTAA LVRDTTFITIRGDNTERALV* -1.809 0.000436 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23597 TVLPYRPHWADTDNNFVLQN 20 SLAY-screened peptide P1947 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTCCTTCCTTATCGCCCTCACTGGGCGGATACGGATAATAACTTTGTTCTGCAGAACTAA TVLPYRPHWADTDNNFVLQN* -1.809 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23598 STSTE 5 SLAY-screened peptide P1948 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGACGTCTACGGAGTAGGCCACTCAGCACCGGTGCATCGCCTTCGTGTCCTACCATTGTTAA STSTE*ATQHRCIAFVSYHC* -1.809 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23599 IRHG 4 SLAY-screened peptide P1949 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAGGCACGGCTAGATTTTCGCTCCCGTTACTAATATTCACTATAGGAATAATATTGCTAAC IRHG*IFAPVTNIHYRNNIAN -1.809 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23600 NLIKGFARLFVCLFQSRFCR 20 SLAY-screened peptide P1950 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCTTATCAAGGGCTTCGCTCGTCTTTTCGTTTGTCTTTTCCAGTCCCGGTTTTGCCGGTAA NLIKGFARLFVCLFQSRFCR* -1.808 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23601 CNTTFPPYFILPILHRNHHM 20 SLAY-screened peptide P1951 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAATACCACCTTTCCCCCGTATTTCATTCTGCCCATTTTGCATCGCAACCATCATATGTAA CNTTFPPYFILPILHRNHHM* -1.808 0.023565 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23602 VSSMGPTLLTSLSGQLKQTY 20 SLAY-screened peptide P1952 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTCTAGTATGGGCCCTACCCTGCTCACTAGTCTCTCCGGCCAGCTCAAGCAGACCTATTAA VSSMGPTLLTSLSGQLKQTY* -1.808 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23603 WALAIYSLAHNVDESDWRTL 20 SLAY-screened peptide P1953 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGGCCCTTGCTATTTACAGTCTCGCTCATAACGTTGATGAGTCGGACTGGCGCACTCTTTAA WALAIYSLAHNVDESDWRTL* -1.807 9.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23604 HSYNRGRPKNSTYS 14 SLAY-screened peptide P1954 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAGCTACAATAGGGGTCGTCCTAAGAATAGCACCTACTCTTAGGTTAACCCTTTTCAGTAA HSYNRGRPKNSTYS*VNPFQ* -1.807 0.000138 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23605 PWSPPWVILRPAILLFASTRN 21 SLAY-screened peptide P1955 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGGTCCCCACCCTGGGTTATCCTCAGGCCCGCCATTCTACTGTTTGCAAGCACGCGTAAC PWSPPWVILRPAILLFASTRN -1.807 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23606 DLSPCLTAPPVTTLDPLAV 19 SLAY-screened peptide P1956 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCTCAGTCCCTGTCTTACGGCCCCTCCCGTTACGACCCTGGACCCTCTCGCGGTGTAGTAA DLSPCLTAPPVTTLDPLAV** -1.806 0.00163 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23607 TAASLSSVANSQLLSENAFT 20 SLAY-screened peptide P1957 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGCTGCCAGCCTCTCCTCCGTGGCTAACTCCCAGCTGCTTTCGGAGAACGCTTTCACGTAA TAASLSSVANSQLLSENAFT* -1.806 0.006977 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23608 THIDQISPCYLHVSDS 16 SLAY-screened peptide P1958 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCACATTGACCAGATTTCCCCCTGCTATCTGCATGTGAGTGATTCTTAGCAGCACGCCTAA THIDQISPCYLHVSDS*QHA* -1.806 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23609 DICQAHNDRQS 11 SLAY-screened peptide P1959 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACATTTGTCAGGCCCACAACGATAGGCAGAGCTAGTAGCGGAAGTGCTTCCGGGATAACTAA DICQAHNDRQS**RKCFRDN* -1.806 0.006853 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23610 DDSTKDPYRLLCFRNYSMNL 20 SLAY-screened peptide P1960 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGACTCCACCAAGGACCCCTACCGTCTCCTGTGCTTTCGCAACTACTCTATGAATCTTTAA DDSTKDPYRLLCFRNYSMNL* -1.806 0.001398 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23611 TPRTFLTRTLTRMCNYVVDH 20 SLAY-screened peptide P1961 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCTCGGACCTTCCTTACCAGGACCCTTACGCGTATGTGCAATTACGTTGTCGATCACTAA TPRTFLTRTLTRMCNYVVDH* -1.805 0.00012 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23612 SREPHSIHNNTCWQATLMIA 20 SLAY-screened peptide P1962 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCGCGAGCCCCACTCGATCCACAACAATACCTGTTGGCAGGCGACGCTTATGATTGCCTAA SREPHSIHNNTCWQATLMIA* -1.805 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23613 RLVGPDSLGTHSLAQYPTRP 20 SLAY-screened peptide P1963 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTTGTGGGTCCCGACAGCCTTGGTACGCATTCTCTGGCTCAGTACCCTACCAGGCCGTAA RLVGPDSLGTHSLAQYPTRP* -1.805 0.017147 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23614 HAILLIYLTLLIPSRTSARE 20 SLAY-screened peptide P1964 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGCCATCTTGCTTATCTATCTTACCCTGCTCATCCCTTCGCGGACTTCCGCCCGCGAGTAA HAILLIYLTLLIPSRTSARE* -1.804 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23615 NQRFRSIHARLTGQNYWMLN 20 SLAY-screened peptide P1965 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCAGCGCTTCCGGTCCATTCACGCTCGGCTTACTGGCCAGAACTATTGGATGTTGAACTAA NQRFRSIHARLTGQNYWMLN* -1.804 0.001502 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23616 PMVEFHVPLAYRTTDENHRV 20 SLAY-screened peptide P1966 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATGGTGGAGTTTCATGTTCCCCTTGCCTACAGGACCACCGACGAGAATCACCGCGTTTAA PMVEFHVPLAYRTTDENHRV* -1.804 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23617 ILYWRPNRSTWAFTVTPIMV 20 SLAY-screened peptide P1967 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCTCTACTGGCGTCCCAACCGCTCCACTTGGGCCTTCACCGTTACTCCCATCATGGTTTAA ILYWRPNRSTWAFTVTPIMV* -1.804 0.00586 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23618 ANSHHLYHLDHGFHNPTIRCN 21 SLAY-screened peptide P1968 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTAATAGTCATCACCTGTATCATCTTGACCACGGGTTTCACAATCCAACTATAAGGTGTAAC ANSHHLYHLDHGFHNPTIRCN -1.804 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23619 CYTPLYMFAVNLITDFSTIA 20 SLAY-screened peptide P1969 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTACACTCCTCTGTATATGTTCGCCGTTAATCTTATCACGGACTTCTCGACGATCGCCTAA CYTPLYMFAVNLITDFSTIA* -1.804 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23620 LWENPSIHFVSGFSVSITYL 20 SLAY-screened peptide P1970 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTGGGAGAATCCTTCCATCCACTTCGTTTCCGGGTTCTCGGTGTCTATTACCTACCTTTAA LWENPSIHFVSGFSVSITYL* -1.804 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23621 KTWEVLTEFRLYTNILWTWD 20 SLAY-screened peptide P1971 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGACCTGGGAGGTTCTCACTGAGTTTCGGCTTTATACTAATATTCTTTGGACGTGGGATTAA KTWEVLTEFRLYTNILWTWD* -1.803 5.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23622 HQRPSNMDC 9 SLAY-screened peptide P1972 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCAGAGGCCCTCCAATATGGATTGCTAGGCCAGGCGTCAGGAGGTCCGCCGTAGTAGTTAA HQRPSNMDC*ARRQEVRRSS* -1.803 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23623 TVAHLFNITNIHTNNLT 17 SLAY-screened peptide P1973 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTTGCCCACCTTTTTAACATTACGAATATCCACACGAACAATCTTACGTAGGACTCGTAA TVAHLFNITNIHTNNLT*DS* -1.803 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23624 LNDKDKTYAVLHIPYNWTPT 20 SLAY-screened peptide P1974 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAATGACAAGGACAAGACTTATGCGGTTCTCCACATTCCCTACAATTGGACGCCTACCTAA LNDKDKTYAVLHIPYNWTPT* -1.802 0.000383 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23625 MGLSPYNCTFVNPSHDMLQI 20 SLAY-screened peptide P1975 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGGGCCTGTCTCCTTACAATTGTACCTTCGTTAACCCTTCTCATGATATGCTTCAGATCTAA MGLSPYNCTFVNPSHDMLQI* -1.801 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23626 CRKPVPTHVFTHFGYSIQIS 20 SLAY-screened peptide P1976 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCGTAAGCCTGTTCCCACGCACGTGTTTACTCACTTCGGTTATAGCATCCAGATCTCGTAA CRKPVPTHVFTHFGYSIQIS* -1.8 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23627 WTNTPHFYSA 10 SLAY-screened peptide P1977 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGACGAACACCCCCCACTTTTATAGTGCGTAGGAGAGGGTTGATACCACCACGCTGATCTAA WTNTPHFYSA*ERVDTTTLI* -1.8 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23628 LDPMPVLDPVLIEAHTAATR 20 SLAY-screened peptide P1978 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGATCCTATGCCTGTTCTTGATCCTGTGCTCATTGAGGCCCATACCGCCGCTACGCGCTAA LDPMPVLDPVLIEAHTAATR* -1.799 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23629 HAA 3 SLAY-screened peptide P1979 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGCTGCTTAGCTTCACCGTAGTATGCCTTACAACAGTAACCCCTATTTTCCCACCAATTAA HAA*LHRSMPYNSNPYFPTN* -1.798 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23630 RINVPRGGFTKAFMFRPPPR 20 SLAY-screened peptide P1980 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGATCAACGTTCCCAGGGGCGGTTTCACCAAGGCGTTTATGTTCCGCCCGCCTCCTCGGTAA RINVPRGGFTKAFMFRPPPR* -1.798 0.007336 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23631 CRCTMPNLHLDALYMSFCPA 20 SLAY-screened peptide P1981 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCGGTGCACTATGCCTAATCTCCACTTGGACGCTCTTTACATGAGTTTCTGTCCTGCTTAA CRCTMPNLHLDALYMSFCPA* -1.798 0.012708 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23632 TWYQCHLYDPHCFKLAP 17 SLAY-screened peptide P1982 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTGGTACCAGTGCCATCTCTATGACCCTCACTGTTTCAAGTTGGCGCCTTAGAACGATTAA TWYQCHLYDPHCFKLAP*ND* -1.797 0.0014 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23633 LKTTIDLSPVVLCGPNLHKT 20 SLAY-screened peptide P1983 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAAGACTACGATCGACCTGTCTCCGGTCGTCCTCTGCGGCCCTAATTTGCATAAGACCTAA LKTTIDLSPVVLCGPNLHKT* -1.797 0.001574 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23634 VPSLDTVGCIPVTDNNHSLT 20 SLAY-screened peptide P1984 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCCGAGTCTTGATACTGTGGGGTGTATCCCTGTTACTGATAATAACCATTCCCTTACGTAA VPSLDTVGCIPVTDNNHSLT* -1.797 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23635 YSVHSTHHVLEQSSAFNNNI 20 SLAY-screened peptide P1985 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACAGTGTTCACAGTACTCATCACGTCCTTGAGCAGTCTAGTGCGTTTAACAACAACATCTAA YSVHSTHHVLEQSSAFNNNI* -1.797 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23636 SAPFLVRLPSMFT 13 SLAY-screened peptide P1986 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGCCCCGTTCTTGGTCAGACTCCCCTCGATGTTTACTTGACGACTTTTCAGAGCCGCTAAC SAPFLVRLPSMFT*RLFRAAN -1.797 0.000347 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23637 EG 2 SLAY-screened peptide P1987 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGGTTAGCGTTAGAGTCTCTAGCGCCCTCAGCTCATTTGGAAGCCTTTTTCGAATGACTAA EG*R*SL*RPQLIWKPFSND* -1.796 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23638 RNNTFHFPKCLNKSFFHMQY 20 SLAY-screened peptide P1988 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAACAATACTTTTCATTTCCCTAAGTGCCTGAATAAGAGTTTCTTTCACATGCAGTATTAA RNNTFHFPKCLNKSFFHMQY* -1.796 0.000681 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23639 FSCSNPNPSYSSLPFTWWYL 20 SLAY-screened peptide P1989 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTCTTGCTCGAACCCTAACCCTTCCTATAGCTCCCTGCCGTTCACGTGGTGGTATCTCTAA FSCSNPNPSYSSLPFTWWYL* -1.796 0.00251 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23640 DYTHVFTFHLI 11 SLAY-screened peptide P1990 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTATACGCACGTGTTTACCTTCCATCTTATTTAGTCTACTAACATGTGTATCTGCTGTTAA DYTHVFTFHLI*STNMCICC* -1.795 0.003063 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23641 REAFRMVYF 9 SLAY-screened peptide P1991 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGAGGCGTTTCGGATGGTTTACTTCTAGAGGTTCTACGATATCAGAATTCTGTTCCTTAAC REAFRMVYF*RFYDIRILFLN -1.795 0.031861 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23642 PP 2 SLAY-screened peptide P1992 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCCTAGTAGGACTCCTGTTCTGCTTATCATTTTTCGTCTCTCAACATTGATTTTTACTAA PP**DSCSAYHFSSLNIDFY* -1.795 0.010814 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23643 HDQCPSLTR 9 SLAY-screened peptide P1993 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGACCAGTGTCCTAGCCTTACTCGGTAGGCGTGTCCTGCCTCTTTTCAGTCTAATTACTAA HDQCPSLTR*ACPASFQSNY* -1.794 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23644 HYAPPCPRSPFNDYIIVHAI 20 SLAY-screened peptide P1994 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTATGCGCCCCCCTGCCCCAGGAGCCCTTTTAACGATTATATCATCGTTCACGCGATCTAA HYAPPCPRSPFNDYIIVHAI* -1.794 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23645 KGAASSKSTREELAFLHTDI 20 SLAY-screened peptide P1995 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGGGGGCCGCTTCCTCGAAGTCCACGCGGGAGGAGCTGGCTTTCCTTCATACGGATATCTAA KGAASSKSTREELAFLHTDI* -1.794 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23646 SPQESRWYRLQTFII 15 SLAY-screened peptide P1996 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCCTCAGGAGTCTCGCTGGTACCGTCTTCAGACTTTCATCATTTGACCGCCCATAGTTAAC SPQESRWYRLQTFII*PPIVN -1.792 0.009361 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23647 MIAPTTISPPSRASYHNFCG 20 SLAY-screened peptide P1997 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGATTGCCCCTACCACTATTTCGCCTCCTTCCCGCGCCTCGTACCATAACTTCTGCGGTTAA MIAPTTISPPSRASYHNFCG* -1.792 3.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23648 TLFSIACENHAVACRFRRAS 20 SLAY-screened peptide P1998 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCTGTTTTCCATTGCTTGCGAGAATCATGCCGTCGCTTGCAGGTTTCGTCGCGCGTCTTAA TLFSIACENHAVACRFRRAS* -1.792 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23649 DPSSFYTRSAIGCTWCFYYC 20 SLAY-screened peptide P1999 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCCCTCGTCTTTTTACACTCGTAGTGCTATTGGCTGCACCTGGTGCTTTTATTACTGCTAA DPSSFYTRSAIGCTWCFYYC* -1.792 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23650 CPFTTLSLIVCLRLSRWSTCR 21 SLAY-screened peptide P2000 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCGTTTACGACTCTCTCATTAATCGTCTGCTTAAGACTCTCGAGATGGTCGACCTGCAGG CPFTTLSLIVCLRLSRWSTCR -1.792 0.012705 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23651 LHNPHHHANGNYNYDYSTWK 20 SLAY-screened peptide P2001 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACAATCCGCATCACCACGCCAACGGTAACTATAATTACGACTACTCTACCTGGAAGTAA LHNPHHHANGNYNYDYSTWK* -1.791 0.000992 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23652 PLQPNCLFFCLGGSVYYQDHY 21 SLAY-screened peptide P2002 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCCAGCCGAACTGCTTGTTCTTCTGTCTGGGCGGCTCTGTCTATTACCAGGACCATTAC PLQPNCLFFCLGGSVYYQDHY -1.791 3.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23653 IMLHLATFTSQNQGSYGASL 20 SLAY-screened peptide P2003 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCATGCTCCATCTGGCCACCTTCACTAGTCAGAATCAGGGTAGTTACGGGGCGTCTCTTTAA IMLHLATFTSQNQGSYGASL* -1.791 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23654 FNFILMPWHLIICLRTRISI 20 SLAY-screened peptide P2004 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCAACTTCATTCTTATGCCCTGGCATCTCATCATTTGTCTGCGTACTCGGATTTCTATTTAA FNFILMPWHLIICLRTRISI* -1.791 0.022296 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23655 IVMADTPNFTTRYIKYTVKD 20 SLAY-screened peptide P2005 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGTGATGGCGGACACCCCGAACTTTACCACGCGGTACATTAAGTACACTGTTAAGGACTAA IVMADTPNFTTRYIKYTVKD* -1.79 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23656 PYPLQPTNYNSETEPKDDNA 20 SLAY-screened peptide P2006 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTATCCTCTGCAGCCCACTAACTACAATAGTGAGACGGAGCCTAAGGACGACAATGCGTAA PYPLQPTNYNSETEPKDDNA* -1.79 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23657 CLYIRMHHPTHSHCATAYNL 20 SLAY-screened peptide P2007 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTCTACATTAGGATGCATCACCCCACTCATAGCCATTGTGCGACGGCTTATAACCTCTAA CLYIRMHHPTHSHCATAYNL* -1.79 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23658 PCSPFAPYRFPMLRWARVSA 20 SLAY-screened peptide P2008 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGTAGTCCGTTCGCCCCTTATCGGTTTCCCATGCTGAGGTGGGCTCGTGTGAGCGCCTAA PCSPFAPYRFPMLRWARVSA* -1.79 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23659 PSTGCINNENFSTPMPVYII 20 SLAY-screened peptide P2009 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTACCGGTTGTATCAACAACGAGAACTTTTCCACTCCTATGCCTGTGTATATTATTTAA PSTGCINNENFSTPMPVYII* -1.79 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23660 SSSAALFQPPYSLPDSELAN 20 SLAY-screened peptide P2010 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCTTCCGCGGCGCTCTTCCAGCCCCCTTATAGCCTTCCTGACAGTGAGCTTGCGAACTAA SSSAALFQPPYSLPDSELAN* -1.789 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23661 PV 2 SLAY-screened peptide P2011 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGTCTAGCCCCAGTGGCTTCCGCGTCTCCCTCTTATTAACTACCTGTTCGCCCTGCGCTAA PV*PQWLPRLPLINYLFALR* -1.789 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23662 SAGRFIPGTPIMNVMYPCVV 20 SLAY-screened peptide P2012 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGCTGGGCGCTTTATTCCCGGGACCCCTATCATGAATGTCATGTATCCTTGCGTTGTTTAA SAGRFIPGTPIMNVMYPCVV* -1.789 4.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23663 INIILRLILNFFYVFWLANS 20 SLAY-screened peptide P2013 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAACATTATTCTTCGCCTGATCCTGAATTTCTTCTACGTCTTCTGGCTTGCTAATTCTTAA INIILRLILNFFYVFWLANS* -1.789 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23664 DACSYFITYRSITKDTCTCY 20 SLAY-screened peptide P2014 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGCTTGCTCGTACTTCATCACTTACCGTAGTATTACGAAGGATACCTGTACTTGTTATTAA DACSYFITYRSITKDTCTCY* -1.788 0.003591 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23665 TLDFTKPVISVNARSPRTQK 20 SLAY-screened peptide P2015 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTCGATTTCACCAAGCCTGTTATCTCGGTCAATGCGAGGTCTCCCCGCACCCAGAAGTAA TLDFTKPVISVNARSPRTQK* -1.788 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23666 LTDYNSNFIPNSALLLHTHR 20 SLAY-screened peptide P2016 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCACCGACTACAATTCGAACTTTATCCCGAATAGCGCTCTTCTTTTGCATACTCACCGGTAA LTDYNSNFIPNSALLLHTHR* -1.788 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23667 PAISRCFAKRYYATHESTVD 20 SLAY-screened peptide P2017 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCTATCTCTCGCTGTTTTGCCAAGCGCTACTATGCCACCCATGAGTCTACTGTCGACTAA PAISRCFAKRYYATHESTVD* -1.788 0.001598 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23668 RCSPAGADPRHHTSIYDDDG 20 SLAY-screened peptide P2018 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTGTAGCCCTGCCGGGGCTGACCCGCGTCACCATACGTCTATTTACGATGACGACGGTTAA RCSPAGADPRHHTSIYDDDG* -1.787 0.002126 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23669 HCHHVINAKNSSMSSSVDYI 20 SLAY-screened peptide P2019 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGCCACCATGTTATTAATGCCAAGAACAGTAGCATGTCCTCTTCCGTTGACTATATTTAA HCHHVINAKNSSMSSSVDYI* -1.787 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23670 NFRACQPTSNDSDLVN 16 SLAY-screened peptide P2020 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTTTCGGGCGTGTCAGCCTACTTCCAATGACTCGGATCTGGTCAATTAGATTATTTTCTAA NFRACQPTSNDSDLVN*IIF* -1.787 0.000128 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23671 HQAFQHTGSPHTVHPLSHRH 20 SLAY-screened peptide P2021 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCAGGCGTTCCAGCACACTGGCTCGCCGCACACGGTGCACCCCCTTAGTCATAGGCACTAA HQAFQHTGSPHTVHPLSHRH* -1.786 0.000548 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23672 QITYHTAPIRVITNFQVFDG 20 SLAY-screened peptide P2022 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGATTACTTACCACACGGCGCCTATCAGGGTCATTACGAATTTTCAGGTTTTTGACGGTTAA QITYHTAPIRVITNFQVFDG* -1.786 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23673 PRM 3 SLAY-screened peptide P2023 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGTATGTAGCGCATCCCCGGTCCTAACGTCCTTTGCTTGTGCGGTGCCCCCCTCTTGTAA PRM*RIPGPNVLCLCGAPLL* -1.786 0.004325 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23674 VARAHNTLCTSHRLLCFNFY 20 SLAY-screened peptide P2024 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGCCCGTGCGCATAATACCCTTTGTACCAGTCATCGTCTTCTTTGCTTCAATTTTTATTAA VARAHNTLCTSHRLLCFNFY* -1.785 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23675 QHTRSKLFCTLSPNRRIAIY 20 SLAY-screened peptide P2025 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCATACGCGCTCTAAGCTTTTCTGCACTCTTTCTCCTAACCGTCGGATCGCCATCTATTAA QHTRSKLFCTLSPNRRIAIY* -1.785 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23676 PHLQSWGSRHPPLVSCDNTS 20 SLAY-screened peptide P2026 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCACTTGCAGAGTTGGGGGTCCCGCCATCCTCCCCTCGTCTCCTGTGACAATACTTCTTAA PHLQSWGSRHPPLVSCDNTS* -1.785 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23677 LSIIICMIYLPFKMSVIQTD 20 SLAY-screened peptide P2027 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGAGCATTATCATCTGTATGATCTATCTCCCCTTTAAGATGTCTGTCATTCAGACTGATTAA LSIIICMIYLPFKMSVIQTD* -1.785 8.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23678 GPTPRCTSHSWTSPPMIFII 20 SLAY-screened peptide P2028 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCCCACCCCGCGCTGTACCTCGCACAGCTGGACTTCTCCGCCGATGATTTTCATTATTTAA GPTPRCTSHSWTSPPMIFII* -1.785 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23679 ARIT 4 SLAY-screened peptide P2029 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCGGATCACTTAGAGTTGGATCCTGCCGACTACCACTTCCCAGCATACTCGTGTTTTTTAA ARIT*SWILPTTTSQHTRVF* -1.785 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23680 PAPVLGSLIRTTPPGAPCFH 20 SLAY-screened peptide P2030 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCTCCTGTCTTGGGGAGTCTCATTCGGACTACTCCTCCTGGTGCCCCGTGTTTCCATTAA PAPVLGSLIRTTPPGAPCFH* -1.784 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23681 SLTYQYATPVSNNMSYADLD 20 SLAY-screened peptide P2031 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCTTACCTACCAGTATGCGACTCCTGTTAGCAATAATATGTCGTACGCTGATCTTGATTAA SLTYQYATPVSNNMSYADLD* -1.784 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23682 PLSRSPTRSAHRYGNRDSNA 20 SLAY-screened peptide P2032 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTTTCCCGTAGCCCGACGCGTAGTGCGCATCGCTACGGCAATCGGGACTCTAATGCTTAA PLSRSPTRSAHRYGNRDSNA* -1.784 0.000172 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23683 SASHSTGYLYYPKSPCTNAN 20 SLAY-screened peptide P2033 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGCGTCTCATTCCACCGGTTACCTCTATTATCCTAAGTCTCCTTGCACCAACGCTAACTAA SASHSTGYLYYPKSPCTNAN* -1.784 0.002517 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23684 CAALFPRPHHVPPDPLDPDT 20 SLAY-screened peptide P2034 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGCTGCCTTGTTTCCGCGTCCGCATCATGTTCCGCCTGATCCCCTCGATCCCGATACGTAA CAALFPRPHHVPPDPLDPDT* -1.784 0.002706 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23685 APPFGHPYICSKSG 14 SLAY-screened peptide P2035 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCCGCCCTTTGGCCACCCCTATATTTGCTCTAAGTCTGGGTAGCGGTTTACTCGTGATTAA APPFGHPYICSKSG*RFTRD* -1.784 0.007807 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23686 YYTGPTTHNWHFALESDSRD 20 SLAY-screened peptide P2036 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTATACTGGGCCGACCACCCATAACTGGCACTTCGCCCTGGAGTCTGATTCTAGGGACTAA YYTGPTTHNWHFALESDSRD* -1.783 0.01191 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23687 PLDYHTARRARCYHSFGHYH 20 SLAY-screened peptide P2037 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCGACTATCATACTGCCCGCCGCGCTCGTTGTTACCACAGTTTTGGGCATTATCATTAA PLDYHTARRARCYHSFGHYH* -1.783 0.001239 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23688 ICFQRLYNQALYHATLRTRH 20 SLAY-screened peptide P2038 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTGCTTCCAGCGCCTCTATAACCAGGCTCTCTATCACGCTACTCTCAGGACTCGGCACTAA ICFQRLYNQALYHATLRTRH* -1.782 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23689 REEHTHYACSFINNVPNRWY 20 SLAY-screened peptide P2039 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGAGGAGCACACCCATTATGCTTGCTCGTTTATTAATAATGTTCCCAACCGCTGGTACTAA REEHTHYACSFINNVPNRWY* -1.782 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23690 KTQQNFGI 8 SLAY-screened peptide P2040 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGACGCAGCAGAACTTTGGGATTTAGAAGAAGAACCATCGCAATCCGTTGAAGTTGCTTTAA KTQQNFGI*KKNHRNPLKLL* -1.782 0.004999 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23691 NSCPLAPIVRATYTWSIVVC 20 SLAY-screened peptide P2041 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTCGTGCCCCTTGGCCCCTATTGTTCGTGCCACCTACACTTGGTCGATCGTCGTTTGTTAA NSCPLAPIVRATYTWSIVVC* -1.781 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23692 VITTFSNKTSSGHRPTCTAR 20 SLAY-screened peptide P2042 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTATTACCACCTTTTCGAACAAGACGTCGTCTGGCCACAGGCCCACGTGCACTGCTCGTTAA VITTFSNKTSSGHRPTCTAR* -1.781 0.004203 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23693 SDSTSCDLVFEYSLI 15 SLAY-screened peptide P2043 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGACTCTACCTCCTGTGACCTGGTCTTTGAGTACAGTCTTATCTAGTTCCCTTGGGTGTAA SDSTSCDLVFEYSLI*FPWV* -1.78 0.020452 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23694 YQFHLHPPNVRPSIVYTVRY 20 SLAY-screened peptide P2044 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCAGTTTCATCTTCATCCTCCGAATGTTCGCCCGTCTATCGTCTATACCGTGCGGTACTAA YQFHLHPPNVRPSIVYTVRY* -1.78 0.008137 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23695 SPQQANNAIRAYFNALYDCN 20 SLAY-screened peptide P2045 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCCTCAGCAGGCGAATAATGCCATCCGGGCTTACTTTAATGCTCTCTATGACTGCAATTAA SPQQANNAIRAYFNALYDCN* -1.78 0.004334 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23696 RNMMFPDCLGGNPKVSPILFN 21 SLAY-screened peptide P2046 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAATATGATGTTTCCGGATTGCCTTGGCGGTAACCCCAAGGTCTCTCCAATACTTTTTAAC RNMMFPDCLGGNPKVSPILFN -1.78 0.005228 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23697 FPAPNYV 7 SLAY-screened peptide P2047 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCCCCGCGCCTAATTACGTGTAGGGCCGTTGGCAGTTCTACTCGCAGACCCCTAAGATCTAA FPAPNYV*GRWQFYSQTPKI* -1.779 0.012605 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23698 FSVNTTLAQAEVIGYHDI 18 SLAY-screened peptide P2048 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTCCGTCAATACTACCCTTGCGCAGGCGGAGGTGATTGGCTACCACGACATTTAGCATTAA FSVNTTLAQAEVIGYHDI*H* -1.779 0.011524 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23699 TAHHFLIPLVPTKMILFSRH 20 SLAY-screened peptide P2049 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGCCCATCATTTCCTTATTCCTCTGGTTCCTACTAAGATGATCCTGTTCTCGCGTCACTAA TAHHFLIPLVPTKMILFSRH* -1.779 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23700 PLLTRATISLHTHTPQDANR 20 SLAY-screened peptide P2050 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCTTGACGCGCGCCACGATTTCTCTGCACACGCACACGCCCCAGGACGCTAACCGCTAA PLLTRATISLHTHTPQDANR* -1.779 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23701 AFTCYITHYSTPSNQRTMNT 20 SLAY-screened peptide P2051 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTTTTACTTGTTACATTACCCATTATTCTACCCCGTCGAATCAGCGTACTATGAACACCTAA AFTCYITHYSTPSNQRTMNT* -1.778 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23702 QVSFNPSSTTYTD 13 SLAY-screened peptide P2052 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGTTAGCTTTAATCCTTCGTCGACTACGTATACTGATTAGTGGTTCATGGGTAATGACTAA QVSFNPSSTTYTD*WFMGND* -1.778 0.000625 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23703 DIQIDRTHHNFKITLTLTCCN 21 SLAY-screened peptide P2053 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATATTCAGATTGATCGCACGCACCATAATTTCAAAATCACCCTAACCCTCACGTGTTGTAAC DIQIDRTHHNFKITLTLTCCN -1.778 0.009228 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23704 PRLDTCT 7 SLAY-screened peptide P2054 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGGTTGGACACTTGTACTTAGATTCCCGCTAATGCTGGGACGGTGTCGGATCATGTCTAA PRLDTCT*IPANAGTVSDHV* -1.778 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23705 HYRCHHSHRAPGLGTIIILIN 21 SLAY-screened peptide P2055 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTACCGTTGTCACCACAGCCACCGGGCTCCCGGTTTGGGAACAATTATAATTCTTATTAAC HYRCHHSHRAPGLGTIIILIN -1.778 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23706 EDLEMVQRGLRQDRERPLS 19 SLAY-screened peptide P2056 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGACCTCGAGATGGTGCAGCGGGGCCTGCGGCAGGATCGGGAGCGGCCCCTCAGCTAGTAA EDLEMVQRGLRQDRERPLS** -1.778 0.006193 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23707 HCLPLP 6 SLAY-screened peptide P2057 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTGCCTGCCCTTGCCCTAGCCGAACAGGCCTATTACCACCGTGACGCTTTGTCGGCCTTAA HCLPLP*PNRPITTVTLCRP* -1.778 0.000392 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23708 GKHSTVLLFRPHFHSDCIGA 20 SLAY-screened peptide P2058 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGAAGCACTCGACTGTTCTCCTGTTTCGCCCCCATTTTCATTCGGATTGTATCGGCGCTTAA GKHSTVLLFRPHFHSDCIGA* -1.778 0.001216 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23709 RNTVRPAIKALHVRKNQ 17 SLAY-screened peptide P2059 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGAACACCGTGCGCCCCGCCATTAAGGCTCTGCACGTTCGCAAGAACCAGTAGAACCTCTAA RNTVRPAIKALHVRKNQ*NL* -1.778 0.029668 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23710 SHNAPTQRNVAIATTRLRVA 20 SLAY-screened peptide P2060 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCACAACGCCCCTACTCAGCGCAACGTCGCCATCGCGACGACTAGGCTCCGTGTTGCGTAA SHNAPTQRNVAIATTRLRVA* -1.777 0.016337 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23711 DVNSYRPNDHPYFPMQILSF 20 SLAY-screened peptide P2061 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGTTAATTCTTATCGTCCGAATGATCACCCCTACTTTCCTATGCAGATTCTCTCGTTCTAA DVNSYRPNDHPYFPMQILSF* -1.777 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23712 YFSQYFFKPCGDPSEYAKIV 20 SLAY-screened peptide P2062 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTTTTCGCAGTATTTCTTTAAGCCCTGCGGCGACCCGTCGGAGTATGCCAAGATCGTCTAA YFSQYFFKPCGDPSEYAKIV* -1.777 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23713 RAVNDISRRQ 10 SLAY-screened peptide P2063 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCCGTGAACGACATCTCTCGGCGCCAGTAGGGTACTCTCAGTCAGGGAGCTACCTTTAAC RAVNDISRRQ*GTLSQGATFN -1.777 0.000888 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23714 RTPLSSTGATALANVAY 17 SLAY-screened peptide P2064 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTACCCCCCTTAGCTCTACCGGGGCGACGGCTCTTGCTAACGTTGCCTATTAGCCTATTTAA RTPLSSTGATALANVAY*PI* -1.777 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23715 HKTTYDPDLVVCLSHSTSPH 20 SLAY-screened peptide P2065 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAAGACGACGTACGATCCCGATCTCGTGGTTTGCCTGTCCCATTCGACTTCCCCCCACTAA HKTTYDPDLVVCLSHSTSPH* -1.777 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23716 LTTVTQSALPTTSVLPILQNY 21 SLAY-screened peptide P2066 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCACTACTGTCACTCAGTCTGCTCTGCCTACCACCTCGGTTTTGCCCATTCTGCAGAATTAT LTTVTQSALPTTSVLPILQNY -1.776 0.014123 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23717 PIRLIAYDLLGYVMGN 16 SLAY-screened peptide P2067 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCATCAGGCTCATCGCCTATGACCTCCTTGGGTACGTCATGGGTAATTAGAACACCCCTTAA PIRLIAYDLLGYVMGN*NTP* -1.776 0.012248 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23718 PPSDHTWTAHHYHLSPGHPH 20 SLAY-screened peptide P2068 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTAGCGATCATACTTGGACCGCCCACCATTATCACTTGAGCCCTGGGCACCCTCATTAA PPSDHTWTAHHYHLSPGHPH* -1.775 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23719 PDTVETAPWAGSPNQCRTTS 20 SLAY-screened peptide P2069 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGATACGGTTGAGACCGCGCCTTGGGCTGGGAGCCCGAATCAGTGTCGTACTACGTCCTAA PDTVETAPWAGSPNQCRTTS* -1.775 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23720 FAFLTWPYYVLNLVVHNKDS 20 SLAY-screened peptide P2070 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGCGTTTCTGACTTGGCCTTACTACGTTCTGAACCTCGTTGTCCATAACAAGGATTCCTAA FAFLTWPYYVLNLVVHNKDS* -1.775 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23721 KSLQTCQRRHCRIHVWYTNS 20 SLAY-screened peptide P2071 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGTCGTTGCAGACCTGTCAGAGGCGCCACTGCAGGATTCACGTGTGGTATACGAATAGTTAA KSLQTCQRRHCRIHVWYTNS* -1.775 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23722 LLT 3 SLAY-screened peptide P2072 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCTTACTTAGGTCTGCCCGCTTAATTCCCTCCCTACTATCTCTATTTACTGCTGTTGTTAA LLT*VCPLNSLPTISIYCCC* -1.775 0.014628 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23723 LERVPCFICTTTSLTYYIRM 20 SLAY-screened peptide P2073 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGAGCGGGTGCCCTGTTTTATCTGCACTACTACCTCTCTCACTTATTATATCAGGATGTAA LERVPCFICTTTSLTYYIRM* -1.775 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23724 TTMNRNTFTDYTCRSVPLFI 20 SLAY-screened peptide P2074 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGACTATGAACCGTAATACTTTCACCGATTATACTTGCAGGTCGGTTCCCTTGTTTATCTAA TTMNRNTFTDYTCRSVPLFI* -1.775 0.017721 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23725 NCNSFPFYGLLYHNVDASLT 20 SLAY-screened peptide P2075 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTGTAATTCCTTTCCTTTCTACGGCCTGCTCTATCACAACGTTGATGCTAGCCTCACGTAA NCNSFPFYGLLYHNVDASLT* -1.774 0.000399 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23726 IVVAVVEISNHRDVQSSEKL 20 SLAY-screened peptide P2076 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGTTGTCGCTGTTGTGGAGATTAGCAATCACAGGGATGTCCAGAGTTCTGAGAAGTTGTAA IVVAVVEISNHRDVQSSEKL* -1.774 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23727 IMQHCRTQLCIIMYTTLTSPN 21 SLAY-screened peptide P2077 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCATGCAGCACTGCCGGACCCAGCTGTGCATTATTATGTATACTACACTTACGTCACCTAAC IMQHCRTQLCIIMYTTLTSPN -1.773 0.000394 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23728 VHVDRLPRTLSCTRSIT 17 SLAY-screened peptide P2078 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCATGTTGACCGTCTTCCTCGCACTCTAAGCTGCACGCGTAGTATTACTTGACCCCTTAAC VHVDRLPRTLSCTRSIT*PLN -1.773 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23729 PTPWCFLHRTSRPKRRTPAA 20 SLAY-screened peptide P2079 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACCCCTTGGTGCTTCCTTCATCGCACCTCCCGTCCTAAGAGGCGCACTCCCGCTGCCTAA PTPWCFLHRTSRPKRRTPAA* -1.773 0.003652 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23730 PCFDNRDNILLDYWFHSAII 20 SLAY-screened peptide P2080 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGCTTTGACAATCGGGATAATATTTTGCTTGATTATTGGTTCCATAGCGCTATTATTTAA PCFDNRDNILLDYWFHSAII* -1.773 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23731 GLSTLPPSVRSQRLSFTLNH 20 SLAY-screened peptide P2081 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCTTTCCACCCTCCCCCCTTCCGTTCGGAGCCAGCGTCTGTCTTTCACCCTGAACCATTAA GLSTLPPSVRSQRLSFTLNH* -1.773 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23732 RHMHSLTSHAGGRKPARAYY 20 SLAY-screened peptide P2082 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCACATGCATTCCCTTACGTCCCATGCTGGTGGCCGCAAGCCTGCTCGGGCTTATTATTAA RHMHSLTSHAGGRKPARAYY* -1.772 0.004599 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23733 TLPWQLPCLHWA 12 SLAY-screened peptide P2083 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTTCCTTGGCAGCTGCCGTGCCTGCACTGGGCGTAGGTTCAGTGCGACTTTTGGAGTTAA TLPWQLPCLHWA*VQCDFWS* -1.772 0.000781 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23734 PQALVVRLRA 10 SLAY-screened peptide P2084 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCAGGCCCTGGTGGTACGATTAAGGGCCTGACCAGTTTTATATCAAGAGTGATTATTAACT PQALVVRLRA*PVLYQE*LLT -1.772 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23735 VEPVMREVPLICTRVATA 18 SLAY-screened peptide P2085 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGAGCCGGTGATGCGTGAGGTTCCCCTCATCTGTACGAGAGTTGCAACAGCATGAACTAAC VEPVMREVPLICTRVATA*TN -1.772 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23736 VLVPGRGVRCRICPSSMGAR 20 SLAY-screened peptide P2086 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCTGGTCCCCGGGCGTGGTGTTAGGTGTCGGATTTGTCCTAGTAGCATGGGCGCTCGGTAA VLVPGRGVRCRICPSSMGAR* -1.771 0.000241 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23737 WLLTSSHINNTFTAYTTTRC 20 SLAY-screened peptide P2087 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCTCCTGACGTCCTCCCACATTAATAACACCTTTACCGCTTACACTACTACGAGGTGCTAA WLLTSSHINNTFTAYTTTRC* -1.771 0.001836 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23738 TNSILSHLSNCRNLFWYDIY 20 SLAY-screened peptide P2088 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGAACTCGATCCTCTCCCATTTGTCCAACTGTCGCAATCTGTTCTGGTATGACATCTACTAA TNSILSHLSNCRNLFWYDIY* -1.771 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23739 PPGRCGCMPDRVNNRMMPPS 20 SLAY-screened peptide P2089 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTGGCCGTTGCGGGTGCATGCCTGATCGTGTGAACAATCGCATGATGCCTCCGTCGTAA PPGRCGCMPDRVNNRMMPPS* -1.77 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23740 LKPMHPQAGRLLFTKGFTDA 20 SLAY-screened peptide P2090 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAAGCCCATGCACCCTCAGGCCGGCCGGCTTCTCTTCACTAAGGGCTTTACCGACGCCTAA LKPMHPQAGRLLFTKGFTDA* -1.77 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23741 CWHSQHRCFHYGCPPYDFLV 20 SLAY-screened peptide P2091 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTGGCACAGTCAGCACCGTTGTTTTCACTATGGTTGTCCTCCTTACGACTTTCTCGTGTAA CWHSQHRCFHYGCPPYDFLV* -1.769 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23742 PPPSRSVGPILALCRSSAN 19 SLAY-screened peptide P2092 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCCCCTCGAGATCGGTAGGGCCCATATTAGCTCTGTGCCGGAGTTCCGCTAACTGAGTA PPPSRSVGPILALCRSSAN*V -1.769 0.025598 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23743 RLSDNTHTLHRCTARIK 17 SLAY-screened peptide P2093 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTTGTCTGATAATACCCACACGCTTCACAGGTGTACCGCTAGGATCAAGTAGTGCCATTAA RLSDNTHTLHRCTARIK*CH* -1.769 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23744 DFDAVCSPLSSWACPV 16 SLAY-screened peptide P2094 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTTCGACGCCGTCTGTTCTCCTTTGAGCAGCTGGGCTTGCCCGGTCTAGATCACCTACTAA DFDAVCSPLSSWACPV*ITY* -1.768 0.021849 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23745 VSSCWTRGSAGRTSTIFSLF 20 SLAY-screened peptide P2095 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTCTAGTTGTTGGACTCGTGGCAGCGCTGGCCGTACGTCTACGATCTTCTCCCTCTTTTAA VSSCWTRGSAGRTSTIFSLF* -1.768 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23746 EDINPIGPSCYPVRSSRYIV 20 SLAY-screened peptide P2096 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGACATTAATCCCATCGGTCCGTCTTGCTATCCTGTGCGCAGTTCTCGGTACATCGTGTAA EDINPIGPSCYPVRSSRYIV* -1.768 1.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23747 HSPSCLSKSAGRTNNSPEPL 20 SLAY-screened peptide P2097 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTCCCCCTCGTGTCTCAGTAAGTCCGCTGGCCGCACGAATAATAGCCCCGAGCCGCTCTAA HSPSCLSKSAGRTNNSPEPL* -1.767 0.002451 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23748 YRSVLIYLL 9 SLAY-screened peptide P2098 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCGGTCCGTCTTGATCTATCTGTTGTAGTAGTTCATCTAGATCAACAGTCATGTTAGGTAA YRSVLIYLL**FI*INSHVR* -1.767 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23749 CIGGPPTRRCYCQFCHYLPLT 21 SLAY-screened peptide P2099 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTATCGGTGGTCCTCCGACCCGTCGGTGTTATTGTCAATTTTGCCATTATTTACCGCTAACT CIGGPPTRRCYCQFCHYLPLT -1.767 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23750 LFPPRPRAYMHFPYFA 16 SLAY-screened peptide P2100 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTTCCCCCCGCGGCCTCGGGCGTATATGCATTTTCCTTATTTCGCTTAGGACGACCGTTAA LFPPRPRAYMHFPYFA*DDR* -1.767 0.0016 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23751 PHTIRRLARPRAYHLCSVHS 20 SLAY-screened peptide P2101 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCACACGATTAGGCGTCTTGCTCGGCCTCGCGCGTACCACCTCTGTTCTGTTCACTCTTAA PHTIRRLARPRAYHLCSVHS* -1.766 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23752 DSSL 4 SLAY-screened peptide P2102 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTCCTCGCTGTAGATTTTGCTGCGCGACGACCATGATCCCAATGATCCCTACTGGAATTAA DSSL*ILLRDDHDPNDPYWN* -1.766 0.008096 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23753 WMPRSGLT 8 SLAY-screened peptide P2103 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGATGCCCAGGTCTGGCCTTACCTAGAACAGTGATAACCCTTAGACGTTCTATTCCCCTTAA WMPRSGLT*NSDNP*TFYSP* -1.765 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23754 MPTHPIPCITI 11 SLAY-screened peptide P2104 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCCTACTCATCCGATCCCTTGTATTACGATTTAGGACTCTTAGTCCCCCCGGTAGACTTAA MPTHPIPCITI*DS*SPR*T* -1.765 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23755 MHAGDCS 7 SLAY-screened peptide P2105 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCACGCCGGCGATTGCTCTTAGTAGTCTAATCGGAATTACTCTACGATTTTCGTCGACTAA MHAGDCS**SNRNYSTIFVD* -1.765 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23756 QIFLASNDTTPMIANNVLPC 20 SLAY-screened peptide P2106 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGATTTTCTTGGCGAGTAATGACACTACGCCGATGATTGCTAACAACGTTCTTCCCTGTTAA QIFLASNDTTPMIANNVLPC* -1.765 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23757 EKRARRVNA 9 SLAY-screened peptide P2107 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGAAGCGCGCTAGGCGTGTCAACGCTTAGATCGGCCTTAGTATCACTTCCGACACTCCGTAA EKRARRVNA*IGLSITSDTP* -1.764 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23758 PDTCRDRRWPSHSLLIASRL 20 SLAY-screened peptide P2108 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGACACTTGCCGTGATCGTCGTTGGCCCTCTCACAGTCTGCTTATCGCTAGCCGGCTTTAA PDTCRDRRWPSHSLLIASRL* -1.764 0.01722 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23759 SLTEGPRRRQTSLSVSVQGH 20 SLAY-screened peptide P2109 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTTGACGGAGGGTCCTAGGCGCCGGCAGACTTCCCTCTCCGTGTCTGTCCAGGGTCACTAA SLTEGPRRRQTSLSVSVQGH* -1.764 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23760 HYSLSLIRTTANNGCPVVHS 20 SLAY-screened peptide P2110 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTACTCCTTGTCTTTGATCAGGACGACTGCTAACAACGGTTGCCCCGTTGTCCACAGCTAA HYSLSLIRTTANNGCPVVHS* -1.764 0.031754 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23761 WLCRNILWICKDHTARLHSM 20 SLAY-screened peptide P2111 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCTGTGTCGTAATATCCTGTGGATTTGCAAGGACCATACTGCCAGGCTTCATTCGATGTAA WLCRNILWICKDHTARLHSM* -1.763 0.017523 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23762 LFGPCFSRPQTCHHRTLRVT 20 SLAY-screened peptide P2112 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTTTGGTCCTTGTTTCAGTCGCCCCCAGACCTGTCACCACCGTACGCTTCGCGTCACTTAA LFGPCFSRPQTCHHRTLRVT* -1.763 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23763 SHTLSTHYSSPRLSLVSVGS 20 SLAY-screened peptide P2113 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCATACGCTTAGCACTCACTATAGTTCTCCGCGGCTCAGCCTTGTTTCCGTTGGCAGCTAA SHTLSTHYSSPRLSLVSVGS* -1.763 0.019599 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23764 LTICLNTHMGPRVNRLNVSS 20 SLAY-screened peptide P2114 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACTATCTGTCTGAACACGCATATGGGCCCCAGGGTGAATCGTCTCAATGTGTCGAGCTAA LTICLNTHMGPRVNRLNVSS* -1.763 0.000441 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23765 WPVLDIFPEQGISSPDTPNP 20 SLAY-screened peptide P2115 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCCTGTCCTGGATATTTTTCCCGAGCAGGGTATTTCCTCGCCTGACACCCCCAACCCGTAA WPVLDIFPEQGISSPDTPNP* -1.762 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23766 YHVMSPMRITLCPTQSPAPN 20 SLAY-screened peptide P2116 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCACGTCATGTCCCCTATGCGCATCACCCTGTGCCCTACCCAGAGCCCTGCCCCGAATTAA YHVMSPMRITLCPTQSPAPN* -1.762 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23767 SNTKHTSF 8 SLAY-screened peptide P2117 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGAACACGAAGCATACCTCGTTCTAGCCCAGCAATCCCTGGTCTTGTATTAACACGTGGTAA SNTKHTSF*PSNPWSCINTW* -1.761 0.000282 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23768 QPRGPPAPGNHTIGPTHLPL 20 SLAY-screened peptide P2118 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCCGCGGGGCCCGCCGGCCCCTGGTAACCATACGATCGGTCCCACCCACCTTCCCCTGTAA QPRGPPAPGNHTIGPTHLPL* -1.761 0.003555 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23769 APSHATNTTKCTLRPEGWHT 20 SLAY-screened peptide P2119 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCCGTCTCACGCGACGAATACCACCAAGTGCACGCTGCGGCCCGAGGGTTGGCACACCTAA APSHATNTTKCTLRPEGWHT* -1.761 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23770 FSGGARPQSSHLLSNLHTCF 20 SLAY-screened peptide P2120 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCTCCGGTGGTGCTCGCCCTCAGTCTAGCCATCTCCTGTCGAACCTCCACACTTGTTTCTAA FSGGARPQSSHLLSNLHTCF* -1.76 2.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23771 PPRMTNLNEYPVSVHPLRIHL 21 SLAY-screened peptide P2121 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCCGGATGACTAACCTCAACGAGTATCCGGTTAGCGTGCATCCCCTCCGCATCCATTTA PPRMTNLNEYPVSVHPLRIHL -1.76 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23772 LQSYTALPG 9 SLAY-screened peptide P2122 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCAGTCCTACACTGCCTTGCCGGGGTAGCTCTGCCCCAGTTACCTTAATCGCTGCATCTAA LQSYTALPG*LCPSYLNRCI* -1.76 0.037505 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23773 RPCLVIRAALRIRLEFKLRS 20 SLAY-screened peptide P2123 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCGTGTTTGGTTATCCGTGCCGCGCTGCGCATCCGCCTTGAGTTTAAGCTGCGTTCGTAA RPCLVIRAALRIRLEFKLRS* -1.76 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23774 TLLSWSSPGGAACNKQTNAN 20 SLAY-screened peptide P2124 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTTCTCAGCTGGAGTTCGCCGGGTGGTGCTGCCTGTAATAAGCAGACTAATGCCAATTAA TLLSWSSPGGAACNKQTNAN* -1.759 0.000123 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23775 LNQLNDSASWN 11 SLAY-screened peptide P2125 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAATCAGCTTAATGATAGTGCTTCGTGGAACTAGTGCCATGATGGCTGGGTGCTTTATTAA LNQLNDSASWN*CHDGWVLY* -1.759 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23776 IHSRPLSRFHVYTSYCHYGP 20 SLAY-screened peptide P2126 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCACAGCAGGCCGTTGTCGCGGTTTCACGTTTACACTAGTTATTGCCACTATGGTCCCTAA IHSRPLSRFHVYTSYCHYGP* -1.759 0.000291 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23777 YFWSALLPNAITSSPLSLSI 20 SLAY-screened peptide P2127 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTTTTGGTCTGCCCTTTTGCCTAACGCTATTACTAGTTCGCCCCTTTCCTTGTCCATCTAA YFWSALLPNAITSSPLSLSI* -1.758 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23778 SVSFRRTIVPARDYREVGTF 20 SLAY-screened peptide P2128 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGTGTCGTTCCGTCGCACCATCGTCCCGGCGCGCGACTACCGCGAGGTCGGCACGTTTTAA SVSFRRTIVPARDYREVGTF* -1.757 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23779 PTLPHIININLQAFCTLFKY 20 SLAY-screened peptide P2129 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACGCTCCCGCATATTATTAACATTAACCTCCAGGCCTTCTGCACTCTTTTTAAGTATTAA PTLPHIININLQAFCTLFKY* -1.757 4.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23780 CLPVSHITYYPGSTLYVGSP 20 SLAY-screened peptide P2130 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTTGCCGGTTTCTCATATCACGTACTACCCCGGCTCCACCCTGTATGTTGGGAGTCCTTAA CLPVSHITYYPGSTLYVGSP* -1.756 0.042177 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23781 PVSFNN 6 SLAY-screened peptide P2131 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGTTTCCTTCAACAATTAGCTTCTTGGGACGTTTAACTAGATTCACCCTCCTGTCCTGTAA PVSFNN*LLGTFN*IHPPVL* -1.756 0.002337 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23782 LEWFVALYSLTGSFRSRVDH 20 SLAY-screened peptide P2132 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGAGTGGTTCGTGGCCCTGTATTCTTTGACTGGCAGCTTCCGGTCTAGGGTCGACCATTAA LEWFVALYSLTGSFRSRVDH* -1.756 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23783 PCLPTIGIAANSTIIATHAS 20 SLAY-screened peptide P2133 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTGCCTGCCTACCATCGGTATCGCTGCCAACTCTACCATTATTGCCACCCACGCTTCTTAA PCLPTIGIAANSTIIATHAS* -1.755 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23784 ITDPARASSYSTGRPYLFDN 20 SLAY-screened peptide P2134 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCACTGATCCGGCCAGGGCCTCTAGTTACTCGACTGGCCGGCCCTATCTGTTTGATAATTAA ITDPARASSYSTGRPYLFDN* -1.755 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23785 DSIMTSAARGVRWVLCLVCVN 21 SLAY-screened peptide P2135 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACAGCATTATGACATCTGCCGCACGAGGAGTACGATGGGTTTTATGCTTAGTCTGCGTTAAC DSIMTSAARGVRWVLCLVCVN -1.754 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23786 RCYAYEVILFEACCYLHNHV 20 SLAY-screened peptide P2136 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTGCTACGCTTACGAGGTTATCCTTTTTGAGGCTTGTTGTTATCTTCACAACCATGTCTAA RCYAYEVILFEACCYLHNHV* -1.753 0.000351 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23787 ALLNCHRSQYAESSSHRQNI 20 SLAY-screened peptide P2137 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCTCCTGAATTGCCACCGTTCCCAGTACGCCGAGTCCTCGTCTCATCGTCAGAACATTTAA ALLNCHRSQYAESSSHRQNI* -1.753 0.000129 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23788 PSP 3 SLAY-screened peptide P2138 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGCCCTTAGTTGGCGCTCTCCCGCCGCGACCGCAAGTAGTACAGCAATATCAGTACTTAA PSP*LALSRRDRK*YSNIST* -1.753 0.01849 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23789 RTAYPFSDRLKVANFRRAHT 20 SLAY-screened peptide P2139 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACCGCGTACCCCTTCAGCGACCGTCTTAAGGTCGCTAACTTCCGCCGGGCTCACACTTAA RTAYPFSDRLKVANFRRAHT* -1.753 0.026433 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23790 SRFLRAPTDNLTCIPL 16 SLAY-screened peptide P2140 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCGCTTTCTTAGGGCGCCCACTGATAATCTTACTTGCATCCCTTTGTAGATGGGCACTTAA SRFLRAPTDNLTCIPL*MGT* -1.752 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23791 SPTLVLLILCLRTRCCRHGT 20 SLAY-screened peptide P2141 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCCCACCCTCGTTCTTCTCATTCTCTGCCTTAGGACCCGCTGCTGTCGTCACGGGACTTAA SPTLVLLILCLRTRCCRHGT* -1.752 0.000204 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23792 PTTDSLPAGCNFTPLTSQLI 20 SLAY-screened peptide P2142 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACTACCGACAGCCTGCCCGCCGGCTGCAACTTCACCCCGCTGACGAGTCAGCTTATTTAA PTTDSLPAGCNFTPLTSQLI* -1.752 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23793 PPFRPRDSPVHMLGCFSCRY 20 SLAY-screened peptide P2143 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCCTTCCGCCCTCGTGACTCTCCGGTCCACATGCTCGGGTGCTTTTCTTGCCGCTACTAA PPFRPRDSPVHMLGCFSCRY* -1.752 0.004147 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23794 CRLGRPYYFRFLIAFIGEPV 20 SLAY-screened peptide P2144 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAGGTTGGGCAGGCCGTATTATTTCCGTTTTCTTATCGCCTTCATTGGCGAGCCTGTCTAA CRLGRPYYFRFLIAFIGEPV* -1.751 0.000539 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23795 HAVTVFPGYSHNRNYSKSHY 20 SLAY-screened peptide P2145 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGCCGTCACCGTTTTTCCCGGCTATAGCCATAACCGTAACTATAGCAAGAGCCATTACTAA HAVTVFPGYSHNRNYSKSHY* -1.75 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23796 KLSG 4 SLAY-screened peptide P2146 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGTTGTCCGGTTAGCCGCCGCTTACGAAGCATACGGAGTATAAGATTACGAAGATCAAGTAA KLSG*PPLTKHTEYKITKIK* -1.75 0.003504 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23797 FPRASPIHQLKNRTLCTIFY 20 SLAY-screened peptide P2147 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCCCCGGGCGTCTCCCATCCATCAGCTTAAGAATCGTACGCTTTGCACTATTTTCTATTAA FPRASPIHQLKNRTLCTIFY* -1.749 0.007814 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23798 RILNSLSSRIYVLVALVKFR 20 SLAY-screened peptide P2148 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTATTTTGAACAGCCTTTCTTCCCGGATTTATGTGTTGGTTGCCCTTGTCAAGTTCCGTTAA RILNSLSSRIYVLVALVKFR* -1.749 0.000981 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23799 KVYNSTLNYTSPSLTPHPYC 20 SLAY-screened peptide P2149 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGGTGTATAACAGCACGCTGAATTACACGTCCCCCTCCTTGACTCCTCATCCTTATTGTTAA KVYNSTLNYTSPSLTPHPYC* -1.748 0.000425 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23800 YHYRCHRNSPPMPYSFTPRT 20 SLAY-screened peptide P2150 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCATTACCGCTGCCACCGGAATAGTCCCCCTATGCCTTATTCCTTTACTCCTCGCACTTAA YHYRCHRNSPPMPYSFTPRT* -1.748 4.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23801 SAGLYVARIHYQGFSMSHCS 20 SLAY-screened peptide P2151 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGCCGGCCTCTACGTCGCCCGGATCCATTACCAGGGCTTTAGTATGTCGCACTGCTCTTAA SAGLYVARIHYQGFSMSHCS* -1.748 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23802 LEKTHINSDSYFGPTDNICK 20 SLAY-screened peptide P2152 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGAGAAGACCCATATCAATAGCGACTCCTACTTCGGTCCCACGGACAATATTTGCAAGTAA LEKTHINSDSYFGPTDNICK* -1.748 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23803 LPYVATKGAFSDT 13 SLAY-screened peptide P2153 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCCCTATGTGGCTACCAAGGGGGCTTTTTCCGATACTTAGGCTACGAGGAGTCACAGCTAA LPYVATKGAFSDT*ATRSHS* -1.748 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23804 MHPYISHFVIKLEASYVCSS 20 SLAY-screened peptide P2154 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCACCCGTACATCTCGCACTTCGTCATTAAGCTGGAGGCTTCCTATGTCTGTTCGTCGTAA MHPYISHFVIKLEASYVCSS* -1.747 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23805 TALSFVLQCLPCL 13 SLAY-screened peptide P2155 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGCTCTCTCCTTCGTCCTCCAGTGCCTGCCCTGCCTTTAGGTGCCGTTCTGCTTCTTGTAA TALSFVLQCLPCL*VPFCFL* -1.747 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23806 HRVNGHAHVLNPLVCGSSRS 20 SLAY-screened peptide P2156 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAGGGTTAACGGTCACGCCCACGTGCTTAATCCGCTTGTCTGCGGGTCGAGCAGGAGCTAA HRVNGHAHVLNPLVCGSSRS* -1.746 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23807 RGSQDLLILFHFSSPTSTDT 20 SLAY-screened peptide P2157 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGGGTTCCCAGGACCTTCTCATCCTGTTTCATTTCTCTAGTCCCACGTCGACTGACACCTAA RGSQDLLILFHFSSPTSTDT* -1.746 0.022835 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23808 PQRSRITVSSSRVYGALNVI 20 SLAY-screened peptide P2158 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCAGCGCTCTAGGATTACTGTTAGCTCTTCGCGTGTCTATGGGGCCCTTAATGTGATCTAA PQRSRITVSSSRVYGALNVI* -1.745 0.000694 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23809 ESRARSTHTVPR 12 SLAY-screened peptide P2159 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGTCGCGCGCTCGGAGTACCCATACGGTGCCCCGCTAGAGTTTGCTGCCTGATGTCGAGTAA ESRARSTHTVPR*SLLPDVE* -1.745 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23810 SDAHENTVVAVRFSWFNDPA 20 SLAY-screened peptide P2160 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGATGCCCACGAGAACACCGTCGTGGCCGTGCGTTTTTCGTGGTTTAATGACCCTGCGTAA SDAHENTVVAVRFSWFNDPA* -1.744 0.00553 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23811 GCPVYAPYPIYIGTSPMMRT 20 SLAY-screened peptide P2161 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTGCCCTGTTTACGCGCCGTACCCCATCTACATTGGTACTTCCCCCATGATGCGGACCTAA GCPVYAPYPIYIGTSPMMRT* -1.744 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23812 KLLFFLTHDGCNCVYTPLCVY 21 SLAY-screened peptide P2162 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCTTTTGTTTTTCCTGACGCATGATGGGTGTAATTGTGTTTATACTCCTTTGTGTGTGTAC KLLFFLTHDGCNCVYTPLCVY -1.744 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23813 LHAGPQFFNNLFDTACLPNN 20 SLAY-screened peptide P2163 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCACGCCGGCCCGCAGTTTTTCAATAATCTTTTTGACACCGCTTGTCTGCCTAATAATTAA LHAGPQFFNNLFDTACLPNN* -1.744 3.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23814 SLMDVCYYLL 10 SLAY-screened peptide P2164 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCTTATGGACGTGTGTTACTATCTGCTGTAGCCCCGCCCTTAGAGTGACCTCGTCGCCTAA SLMDVCYYLL*PRP*SDLVA* -1.743 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23815 ADLRGYLKEHKRYISIYYCP 20 SLAY-screened peptide P2165 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGACCTCCGCGGCTACCTCAAGGAGCACAAGCGCTATATTAGCATCTACTACTGTCCTTAA ADLRGYLKEHKRYISIYYCP* -1.743 8.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23816 WTYYVLALRPGSLTIGYTRT 20 SLAY-screened peptide P2166 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGACCTACTACGTCCTGGCCCTTCGGCCCGGTTCGCTTACTATTGGCTATACGCGGACGTAA WTYYVLALRPGSLTIGYTRT* -1.743 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23817 PIVTGSTHLTKKALSYGTSA 20 SLAY-screened peptide P2167 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCATCGTCACGGGGTCCACGCATCTGACTAAGAAGGCCTTGAGTTACGGCACTTCTGCCTAA PIVTGSTHLTKKALSYGTSA* -1.742 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23818 TCTTDAQDNSSSVCATRQNA 20 SLAY-screened peptide P2168 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTGCACCACCGACGCTCAGGATAATTCTAGTAGTGTTTGCGCGACCCGCCAGAATGCGTAA TCTTDAQDNSSSVCATRQNA* -1.742 0.004084 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23819 QYEYLPNAHTTYTSISQSWI 20 SLAY-screened peptide P2169 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTATGAGTACTTGCCTAATGCCCATACCACTTATACTTCCATTAGTCAGTCCTGGATCTAA QYEYLPNAHTTYTSISQSWI* -1.742 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23820 VVRCFHSIWRPSSCGSYIIL 20 SLAY-screened peptide P2170 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGTTAGGTGTTTTCACAGCATCTGGCGGCCTAGTAGTTGCGGGTCGTATATCATCCTCTAA VVRCFHSIWRPSSCGSYIIL* -1.742 0.025366 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23821 CSTEVYNRSFEIHFLATLTP 20 SLAY-screened peptide P2171 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTCGACCGAGGTGTATAACCGTAGCTTCGAGATCCACTTTCTTGCTACTCTTACGCCTTAA CSTEVYNRSFEIHFLATLTP* -1.742 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23822 SPATLSHNFIRPLSACLTSY 20 SLAY-screened peptide P2172 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCCGGCCACGCTGAGTCACAATTTTATTCGTCCTCTCTCTGCCTGTCTGACCTCTTACTAG SPATLSHNFIRPLSACLTSY* -1.742 0.011457 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23823 NCSRPCCITVSMIGDARSTF 20 SLAY-screened peptide P2173 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTGTTCCCGCCCGTGCTGTATCACTGTTTCTATGATTGGCGACGCGCGGAGTACCTTCTAA NCSRPCCITVSMIGDARSTF* -1.742 3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23824 PSATRPPL 8 SLAY-screened peptide P2174 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTCTGCTACTCGCCCTCCCCTTTAGCTCACTCCGCCTACGTGTACTAAGGCTTGCCGTTAA PSATRPPL*LTPPTCTKACR* -1.742 3.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23825 RAIPRHRSVTTPGKRPCNCN 20 SLAY-screened peptide P2175 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGCCATCCCTCGTCACCGCTCTGTCACCACCCCCGGCAAGCGTCCTTGTAATTGTAACTAA RAIPRHRSVTTPGKRPCNCN* -1.741 0.004644 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23826 PNIPKDT 7 SLAY-screened peptide P2176 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAACATTCCTAAGGACACTTAGTCTGTCACTCTTAAGATTGTCGATTACATGCCTGATTAA PNIPKDT*SVTLKIVDYMPD* -1.741 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23827 LHRNMLGTFAHPHKMQASID 20 SLAY-screened peptide P2177 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACCGTAACATGCTCGGGACGTTTGCTCATCCCCACAAGATGCAGGCGAGCATTGACTAA LHRNMLGTFAHPHKMQASID* -1.741 0.000508 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23828 TAVCFRARFPQVSYALNTLG 20 SLAY-screened peptide P2178 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGCCGTCTGCTTCCGTGCCAGGTTTCCCCAGGTGAGTTACGCCCTGAATACTCTTGGCTAA TAVCFRARFPQVSYALNTLG* -1.741 0.009043 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23829 TELPQHNLTSTAFALRCFHT 20 SLAY-screened peptide P2179 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGAGCTGCCTCAGCACAACTTGACTTCCACGGCCTTTGCCCTGCGGTGTTTCCATACCTAA TELPQHNLTSTAFALRCFHT* -1.741 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23830 PGADLCNKLAYSTQYPYIPN 20 SLAY-screened peptide P2180 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGGTGCGGATCTCTGCAACAAGTTGGCCTATTCCACTCAGTACCCCTATATTCCCAACTAA PGADLCNKLAYSTQYPYIPN* -1.74 0.02154 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23831 HYNFR 5 SLAY-screened peptide P2181 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTATAATTTTCGTTAGTCGCGCATTACGTGTTCGACGGTGGTTCTGAGTACTCTCATTTAA HYNFR*SRITCSTVVLSTLI* -1.74 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23832 ICSPDMKARYNNPAPQTLTF 20 SLAY-screened peptide P2182 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTGTAGCCCGGATATGAAGGCCCGCTACAATAATCCCGCTCCTCAGACCTTGACCTTTTAA ICSPDMKARYNNPAPQTLTF* -1.74 2.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23833 HICYRETGTLPTITIPYSFI 20 SLAY-screened peptide P2183 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATCTGTTACCGGGAGACGGGCACGTTGCCGACTATCACCATCCCTTATAGCTTTATTTAA HICYRETGTLPTITIPYSFI* -1.739 0.005552 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23834 VLPFRLDPRD 10 SLAY-screened peptide P2184 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCTGCCCTTTCGCCTGGACCCTAGGGACTAGATGGTCACGAGTGCTGTTAACGTTGCTTAA VLPFRLDPRD*MVTSAVNVA* -1.739 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23835 GRLQRVPIVCHAETFVDLAP 20 SLAY-screened peptide P2185 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCGTTTGCAGCGTGTGCCTATTGTCTGCCACGCGGAGACCTTTGTTGACCTGGCGCCCTAA GRLQRVPIVCHAETFVDLAP* -1.739 0.001977 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23836 PDRTPCTNPYNDFRTLHYIL 20 SLAY-screened peptide P2186 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGACCGTACCCCTTGTACCAACCCTTACAACGACTTCAGGACCCTGCACTACATCCTTTAA PDRTPCTNPYNDFRTLHYIL* -1.738 0.001513 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23837 VHNVNGQLYQAH 12 SLAY-screened peptide P2187 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCACAACGTCAATGGGCAGCTGTATCAGGCTCACTAGTTTACCGCCAGCGCCATCCCGTAA VHNVNGQLYQAH*FTASAIP* -1.738 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23838 EPTDDGHLQYFSSPSPLVVLT 21 SLAY-screened peptide P2188 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCCGACGGACGACGGGCACTTACAGTACTTTTCTTCTCCATCCCCCTTAGTTGTCCTAACT EPTDDGHLQYFSSPSPLVVLT -1.738 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23839 SFCNTSLLAPALFPP 15 SLAY-screened peptide P2189 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTTTTGTAATACGTCCTTGCTCGCCCCCGCGCTCTTCCCTCCTTAGGCCATCACGAGTTAA SFCNTSLLAPALFPP*AITS* -1.738 0.000257 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23840 RFVVATIKLTVESRILNVYWL 21 SLAY-screened peptide P2190 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTTTGTTGTGGCGACGATTAAGCTTACGGTGGAGTCCCGGATCCTTAATGTCTACTGGCTA RFVVATIKLTVESRILNVYWL -1.737 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23841 MFGQNILPSNLCTDPPDLDA 20 SLAY-screened peptide P2191 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTTTGGGCAGAACATCCTCCCTTCTAACTTGTGCACCGACCCTCCTGACTTGGATGCCTAA MFGQNILPSNLCTDPPDLDA* -1.737 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23842 SDRAATRVPLCHDHLLSYEE 20 SLAY-screened peptide P2192 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGACCGTGCGGCTACGAGGGTGCCTCTCTGCCATGATCATTTGCTTTCGTACGAGGAGTAA SDRAATRVPLCHDHLLSYEE* -1.737 0.003694 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23843 TRHYNPPTRTFMCHPAGAAP 20 SLAY-screened peptide P2193 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCGGCATTATAACCCGCCTACCCGTACCTTCATGTGTCATCCCGCCGGCGCCGCCCCTTAA TRHYNPPTRTFMCHPAGAAP* -1.737 0.010628 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23844 QSRFSGIHDVLHVALDWLFL 20 SLAY-screened peptide P2194 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGTCGAGGTTTTCGGGTATTCATGATGTGCTTCATGTCGCTCTTGACTGGCTGTTCTTGTAA QSRFSGIHDVLHVALDWLFL* -1.736 0.000234 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23845 RMSPLYHALDLTGWYSI 17 SLAY-screened peptide P2195 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTATGTCTCCTCTTTATCACGCGCTTGACCTTACGGGCTGGTATTCTATTTAGTCTAACTAA RMSPLYHALDLTGWYSI*SN* -1.736 0.043012 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23846 QLLFSRLLLTRILLSLLRRN 20 SLAY-screened peptide P2196 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCTGCTGTTCTCTCGACTCTTACTTACCCGCATCCTACTAAGCCTTCTTCGCCGTAACTGA QLLFSRLLLTRILLSLLRRN* -1.734 0.003339 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23847 PRRRHIDAFIDST 13 SLAY-screened peptide P2197 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGTCGTCGGCATATTGACGCGTTCATCGACAGTACCTAGAATATGAACTACTGTTACTAA PRRRHIDAFIDST*NMNYCY* -1.734 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23848 TYYPATNIRANNPLQSQNMS 20 SLAY-screened peptide P2198 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTACTACCCGGCCACTAACATTCGGGCGAATAACCCGCTCCAGTCTCAGAACATGTCGTAA TYYPATNIRANNPLQSQNMS* -1.734 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23849 GYGQLFCRRDIMCNLKTASA 20 SLAY-screened peptide P2199 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTATGGGCAGCTCTTTTGTCGCCGCGATATTATGTGCAACTTGAAGACTGCTAGTGCGTAA GYGQLFCRRDIMCNLKTASA* -1.733 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23850 LQSNRHGHPTHANSFTHPPI 20 SLAY-screened peptide P2200 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCAGTCCAACAGGCACGGCCATCCCACCCATGCCAATTCTTTTACGCATCCGCCCATCTAA LQSNRHGHPTHANSFTHPPI* -1.733 5.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23851 SRHNVSNFVALNCAPHTDTF 20 SLAY-screened peptide P2201 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCGTCATAACGTCTCCAATTTCGTTGCCCTTAACTGTGCCCCGCACACCGACACGTTTTAA SRHNVSNFVALNCAPHTDTF* -1.733 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23852 NHKTTAGEPCKRCSKFSNCP 20 SLAY-screened peptide P2202 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCACAAGACTACGGCGGGGGAGCCTTGTAAGAGGTGCAGCAAGTTCTCGAATTGTCCCTAA NHKTTAGEPCKRCSKFSNCP* -1.732 0.00191 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23853 FITMPHPDNPGSKEDTHNNA 20 SLAY-screened peptide P2203 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTATTACTATGCCGCATCCTGACAACCCGGGTTCTAAGGAGGATACGCATAACAATGCCTAA FITMPHPDNPGSKEDTHNNA* -1.732 0.005869 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23854 LYDAIRSEHISPLLFTILFFN 21 SLAY-screened peptide P2204 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTACGACGCCATCCGGTCCGAGCATATAAGCCCTCTGCTCTTCACTATCCTTTTCTTTAAC LYDAIRSEHISPLLFTILFFN -1.732 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23855 NV 2 SLAY-screened peptide P2205 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGTGTAGTACTATGCCGCTGTTCGGATGCCTCTTTCCAACTGCCTTAGCGGTGAGCGCTAA NV*YYAAVRMPLSNCLSGER* -1.731 0.020523 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23856 RSEDCDRSTSCNFTCTSYDL 20 SLAY-screened peptide P2206 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTCCGAGGACTGCGACCGTAGTACTTCCTGTAATTTTACCTGCACCTCTTACGACCTTTAA RSEDCDRSTSCNFTCTSYDL* -1.731 0.00015 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23857 CPVRAHYAGFNLDNNMPYDR 20 SLAY-screened peptide P2207 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCCGTGCGCGCCCACTATGCTGGGTTTAATCTCGATAACAACATGCCCTATGATCGCTAA CPVRAHYAGFNLDNNMPYDR* -1.73 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23858 PRHRNLAI 8 SLAY-screened peptide P2208 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGCCACCGTAATCTGGCGATTTAGGATACTCCCTCTAGTAACCAGCAGGGCCATGGGTAA PRHRNLAI*DTPSSNQQGHG* -1.73 0.000166 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23859 VQSRVLRRIITCLFSRFVPV 20 SLAY-screened peptide P2209 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTCAGAGCCGCGTGCTCAGGAGGATCATTACGTGTTTGTTCTCGAGGTTTGTTCCTGTTTAA VQSRVLRRIITCLFSRFVPV* -1.73 0.000224 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23860 WDYNSLSKLTITPWNYMQST 20 SLAY-screened peptide P2210 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGGATTACAATTCCCTGTCCAAGCTCACTATCACCCCCTGGAACTACATGCAGTCGACGTAA WDYNSLSKLTITPWNYMQST* -1.73 0.019816 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23861 DSRYSQSIQDFRPTRNSHNW 20 SLAY-screened peptide P2211 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTCGCGGTATTCCCAGTCTATTCAGGATTTTCGGCCGACGAGGAATTCCCACAATTGGTAA DSRYSQSIQDFRPTRNSHNW* -1.73 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23862 LSSLTLECLPLMTQFDHALSN 21 SLAY-screened peptide P2212 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGAGTTCGCTGACTCTTGAGTGTCTTCCCTTGATGACCCAGTTCGACCACGCACTAAGTAAC LSSLTLECLPLMTQFDHALSN -1.73 0.009395 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23863 RRLSHHLAFPNMRRITLAILN 21 SLAY-screened peptide P2213 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCGCCTCTCGCATCATCTTGCTTTTCCGAACATGAGGAGAATAACGCTAGCCATACTTAAC RRLSHHLAFPNMRRITLAILN -1.729 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23864 HPSESSNCHNLPFWPGSFIE 20 SLAY-screened peptide P2214 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCCCTCTGAGAGCTCTAACTGTCATAACCTTCCTTTCTGGCCTGGCTCCTTCATTGAGTAA HPSESSNCHNLPFWPGSFIE* -1.729 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23865 HLTTALNGPYRVTMALDNIP 20 SLAY-screened peptide P2215 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCTTACCACGGCTCTTAATGGTCCGTACCGGGTTACGATGGCTCTTGACAACATCCCCTAA HLTTALNGPYRVTMALDNIP* -1.729 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23866 LHRNRSSRTPRTTYAGDHPP 20 SLAY-screened peptide P2216 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCACCGGAATAGGTCCTCCCGTACGCCGAGGACCACCTATGCCGGCGACCACCCTCCTTAA LHRNRSSRTPRTTYAGDHPP* -1.729 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23867 GYRIRIPGTVNNHDECYLLA 20 SLAY-screened peptide P2217 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTATCGCATTCGGATTCCCGGTACTGTCAATAATCACGATGAGTGCTATCTTTTGGCGTAA GYRIRIPGTVNNHDECYLLA* -1.729 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23868 IDVGQYQSIPKCVARMLI 18 SLAY-screened peptide P2218 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGACGTTGGCCAGTATCAGAGCATCCCTAAGTGTGTTGCCCGTATGCTCATTTAGTATTAA IDVGQYQSIPKCVARMLI*Y* -1.728 5.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23869 RPAYILEQPRGWVPTEQYLL 20 SLAY-screened peptide P2219 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCGGCTTACATCCTCGAGCAGCCTCGGGGGTGGGTTCCTACCGAGCAGTACCTTCTCTAA RPAYILEQPRGWVPTEQYLL* -1.728 0.039109 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23870 GLSVNHILALRLHRAFYLDP 20 SLAY-screened peptide P2220 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCTTTCCGTGAATCATATCCTTGCCCTTCGCCTTCACCGGGCCTTTTACCTTGACCCTTAA GLSVNHILALRLHRAFYLDP* -1.728 0.004898 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23871 TWHYHCQAPSIWSLTPSYSN 20 SLAY-screened peptide P2221 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGGCATTACCACTGTCAGGCGCCCTCTATCTGGTCCCTCACTCCGAGTTACAGCAATTAA TWHYHCQAPSIWSLTPSYSN* -1.728 6.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23872 PDLTHSPWLNIPWSLVDACA 20 SLAY-screened peptide P2222 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGACCTTACTCACTCCCCGTGGCTCAACATCCCGTGGTCTCTTGTGGATGCCTGTGCCTAA PDLTHSPWLNIPWSLVDACA* -1.728 0.007056 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23873 LCSTYGPTRHGSPSQIPFHT 20 SLAY-screened peptide P2223 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTGCTCTACGTACGGCCCCACGCGCCACGGCAGCCCCTCCCAGATCCCTTTTCATACGTAA LCSTYGPTRHGSPSQIPFHT* -1.727 0.020993 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23874 RIPAKDHKQYRSTLCTVRRSN 21 SLAY-screened peptide P2224 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCATCCCTGCTAAGGACCACAAGCAGTACCGAAGCACGTTATGTACTGTTAGGCGTAGTAAC RIPAKDHKQYRSTLCTVRRSN -1.726 0.000249 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23875 DVRSLVLLN 9 SLAY-screened peptide P2225 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGTCCGCAGTTTGGTGCTTCTCAATTAGAACCCCTCGCGCGCGCGTGCTTTTGTGAAGTAA DVRSLVLLN*NPSRARAFVK* -1.726 0.006629 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23876 PNPPNVWRLAHLDDPSTLTL 20 SLAY-screened peptide P2226 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAATCCGCCGAACGTTTGGCGCCTTGCCCATCTGGATGACCCTAGTACTCTTACGTTGTAA PNPPNVWRLAHLDDPSTLTL* -1.726 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23877 CLPNGVPTPNCYIVHFTALS 20 SLAY-screened peptide P2227 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTCCCCAATGGTGTTCCTACGCCTAACTGCTATATTGTTCATTTCACCGCCCTTTCCTAA CLPNGVPTPNCYIVHFTALS* -1.725 0.000446 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23878 SSPCALYSQISITFLNWVRT 20 SLAY-screened peptide P2228 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTAGCCCCTGTGCGCTCTATTCGCAGATTTCTATCACCTTCCTTAATTGGGTTCGTACTTAA SSPCALYSQISITFLNWVRT* -1.725 0.000119 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23879 TCHAHCLPRNLPTIPICLYR 20 SLAY-screened peptide P2229 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTGTCATGCCCATTGCTTGCCTCGTAATTTGCCGACGATTCCGATTTGCCTCTACCGTTAA TCHAHCLPRNLPTIPICLYR* -1.725 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23880 IPPTRRSNTSHDS 13 SLAY-screened peptide P2230 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCCCCCTACCAGGCGGAGTAATACCTCCCATGACTCTTAGGATATCACTGACTAGCGGTAA IPPTRRSNTSHDS*DITD*R* -1.724 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23881 QNNDV 5 SLAY-screened peptide P2231 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGAACAATGATGTTTAGAACTGGCGTGTCTACCGTTCCTGTCGCTACACTTATACGGATTAA QNNDV*NWRVYRSCRYTYTD* -1.724 0.014278 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23882 PTAVVTSPVPNPFNVYSYTI 20 SLAY-screened peptide P2232 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACCGCCGTGGTCACCAGCCCCGTTCCTAACCCCTTTAACGTTTATTCTTATACTATTTAA PTAVVTSPVPNPFNVYSYTI* -1.724 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23883 PPQPQFGIVRSGVGVHQIGI 20 SLAY-screened peptide P2233 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCTCAGCCTCAGTTTGGCATTGTGCGCTCGGGGGTGGGCGTGCACCAGATCGGTATTTAA PPQPQFGIVRSGVGVHQIGI* -1.724 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23884 PRFSKCIRWNIQHLPIT 17 SLAY-screened peptide P2234 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCGCTTCTCCAAGTGTATCCGTTGGAATATCCAGCATCTCCCCATTACCTAGATCCGCTAA PRFSKCIRWNIQHLPIT*IR* -1.724 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23885 LNSITVLLRLILNVVLLCFS 20 SLAY-screened peptide P2235 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGAATAGCATCACTGTGCTGCTCCGCTTGATCCTTAACGTCGTTTTGCTTTGCTTTTCCTAA LNSITVLLRLILNVVLLCFS* -1.723 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23886 YRPTNANVTTHATLALAVPC 20 SLAY-screened peptide P2236 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCGCCCTACGAATGCGAACGTTACCACCCACGCGACTCTGGCTCTCGCGGTCCCGTGCTAA YRPTNANVTTHATLALAVPC* -1.723 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23887 TLHLCALHF 9 SLAY-screened peptide P2237 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTCCACCTGTGCGCTCTGCATTTTTAGCTGAACCCTCGTCATTCCTTTTAGGTTCCTTAA TLHLCALHF*LNPRHSF*VP* -1.723 0.001475 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23888 HPGAFPPR 8 SLAY-screened peptide P2238 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCCGGCGCTTTTCCCCCGCGCTAGCACCACATCGTTATTAGTCACGGGCGTAAGTCGTAC HPGAFPPR*HHIVISHGRKSY -1.723 0.001259 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23889 PQRPTYNNTCTTRSHYLPGY 20 SLAY-screened peptide P2239 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCAGCGCCCTACCTACAATAATACTTGTACTACGCGCTCGCATTATCTCCCCGGTTACTAA PQRPTYNNTCTTRSHYLPGY* -1.723 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23890 RPRRQSVHEQCHATPIATSCN 21 SLAY-screened peptide P2240 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCTCGCCGTCAGAGCGTCCACGAGCAGTGCCATGCTACTCCTATCGCCACGAGCTGTAAC RPRRQSVHEQCHATPIATSCN -1.722 0.033116 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23891 KPVYIRSSPYCHMGDRRATA 20 SLAY-screened peptide P2241 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCCTGTCTATATTCGGAGTAGCCCCTACTGTCATATGGGTGATCGGCGTGCGACGGCCTAA KPVYIRSSPYCHMGDRRATA* -1.721 0.001061 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23892 MSGTHGWLMEPLFHVLLACA 20 SLAY-screened peptide P2242 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTCTGGTACTCACGGTTGGCTCATGGAGCCGTTGTTTCACGTCCTGCTTGCTTGTGCTTAA MSGTHGWLMEPLFHVLLACA* -1.721 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23893 KSYSE 5 SLAY-screened peptide P2243 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGAGCTATTCGGAGTAGAGCATCGCCAGGCACCTGAGTGGCACCTATCACATTGACTTGTAA KSYSE*SIARHLSGTYHIDL* -1.72 0.000502 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23894 LVKMNRSLILTVINRTNRRW 20 SLAY-screened peptide P2244 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGTCAAGATGAACCGCAGCCTGATCTTGACTGTCATCAACCGGACCAACAGGCGCTGGTAA LVKMNRSLILTVINRTNRRW* -1.72 0.015724 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23895 SARNHQPYTPICLGAASLSI 20 SLAY-screened peptide P2245 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGCGCGTAATCACCAGCCTTATACTCCTATCTGCTTGGGTGCTGCTTCTCTTTCTATTTAA SARNHQPYTPICLGAASLSI* -1.719 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23896 PCSMPPYWWGIAVVPTIEIV 20 SLAY-screened peptide P2246 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTGCTCGATGCCCCCTTACTGGTGGGGTATCGCTGTTGTCCCCACTATCGAGATTGTGTAA PCSMPPYWWGIAVVPTIEIV* -1.719 4.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23897 PLYEAGTIHFPLQP 14 SLAY-screened peptide P2247 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTGTATGAGGCCGGCACCATCCATTTCCCTTTGCAGCCTTAGCATACGAATAACGTGTAA PLYEAGTIHFPLQP*HTNNV* -1.719 0.002428 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23898 RYVPTMHITSCTDWAGCHYL 20 SLAY-screened peptide P2248 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTATGTCCCTACCATGCACATTACGAGTTGTACTGACTGGGCGGGTTGCCACTATCTCTAA RYVPTMHITSCTDWAGCHYL* -1.719 0.001062 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23899 KPH 3 SLAY-screened peptide P2249 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCCTCATTAGCATCCTATCAACTTCTACCTGTAGCATTACGATTAGATGGATGTTTTGTAA KPH*HPINFYL*HYD*MDVL* -1.718 0.004391 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23900 RSGRTQCYNRNYHPFIIEVL 20 SLAY-screened peptide P2250 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTCTGGTCGGACCCAGTGCTACAACCGGAATTATCACCCTTTTATTATTGAGGTCTTGTAA RSGRTQCYNRNYHPFIIEVL* -1.718 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23901 SYHISLASISTSSYGQYPHVT 21 SLAY-screened peptide P2251 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTACCATATATCCCTTGCTTCTATTAGTACATCGTCATACGGGCAGTACCCCCATGTAACT SYHISLASISTSSYGQYPHVT -1.718 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23902 PLRMLTDKKPSFVIMEKFHT 20 SLAY-screened peptide P2252 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCCGGATGTTGACTGACAAGAAGCCTAGTTTTGTCATCATGGAGAAGTTCCATACTTAA PLRMLTDKKPSFVIMEKFHT* -1.718 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23903 RLSRPENPPVRDYILLSWGC 20 SLAY-screened peptide P2253 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTTAGTCGTCCGGAGAATCCTCCCGTCCGTGATTATATCCTTTTGTCTTGGGGCTGCTAA RLSRPENPPVRDYILLSWGC* -1.717 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23904 TCSSFHLRWRALDTTSRCRL 20 SLAY-screened peptide P2254 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTTGTTCGTCTTTTCACCTCCGTTGGAGGGCGTTGGATACGACCTCCCGCTGCCGTCTTTAA TCSSFHLRWRALDTTSRCRL* -1.717 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23905 CRWNYRYPYFYRI 13 SLAY-screened peptide P2255 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGTTGGAACTACAGGTACCCGTACTTCTACCGTATCTAGCACCGCAGGAACACCTTCTAA CRWNYRYPYFYRI*HRRNTF* -1.717 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23906 GSQYACFFYQSGKLLVRTI 19 SLAY-screened peptide P2256 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGAGTCAGTACGCGTGTTTTTTTTATCAGAGTGGCAAGTTGCTTGTTAGGACTATTTAGTAA GSQYACFFYQSGKLLVRTI** -1.717 0.000934 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23907 QVPSWFNPGVYRQILL 16 SLAY-screened peptide P2257 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGTCCCCTCTTGGTTCAACCCTGGCGTCTATCGTCAGATTCTGCTTTAGTGCCTGGCTTAA QVPSWFNPGVYRQILL*CLA* -1.717 0.005903 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23908 TTASPVCHLVPRYTPNTTIS 20 SLAY-screened peptide P2258 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCACGGCTTCGCCCGTTTGCCACCTTGTTCCTAGGTACACGCCGAATACTACTATTTCCTAA TTASPVCHLVPRYTPNTTIS* -1.716 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23909 ITNHSYFPRLGTHSTQTSSC 20 SLAY-screened peptide P2259 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTACCAATCACAGTTATTTCCCGCGCTTGGGCACTCACTCGACCCAGACGTCCTCCTGCTAA ITNHSYFPRLGTHSTQTSSC* -1.716 0.00655 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23910 TLARLTVDVSNLKKNHDGLS 20 SLAY-screened peptide P2260 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCTTGCCAGGCTGACTGTGGATGTCAGCAACTTGAAGAAGAATCATGACGGTTTGTCGTAA TLARLTVDVSNLKKNHDGLS* -1.716 0.012844 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23911 CNSPGTDPEPQYCVPYHKDP 20 SLAY-screened peptide P2261 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAACTCGCCGGGCACCGACCCTGAGCCCCAGTATTGCGTCCCGTATCATAAGGATCCTTAA CNSPGTDPEPQYCVPYHKDP* -1.716 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23912 ALLTASLVLRARIRLSMRNN 20 SLAY-screened peptide P2262 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCTCCTCACTGCCAGTCTTGTCCTGCGTGCCCGCATTCGTCTGTCCATGAGGAATAACTAA ALLTASLVLRARIRLSMRNN* -1.716 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23913 GRLPNHNGVPPSLATTGQCS 20 SLAY-screened peptide P2263 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCGCCTTCCGAACCACAATGGGGTTCCCCCTAGTTTGGCTACTACTGGCCAGTGCTCCTAA GRLPNHNGVPPSLATTGQCS* -1.716 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23914 LALAEHSTSMYPLRNRSSPW 20 SLAY-screened peptide P2264 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGCTCTCGCGGAGCACTCGACCAGTATGTATCCTTTGCGTAATCGCTCCTCGCCGTGGTAA LALAEHSTSMYPLRNRSSPW* -1.715 1.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23915 AIQRELNSTRYLSIARLSSLN 21 SLAY-screened peptide P2265 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCATTCAGCGTGAGCTTAACTCTACGCGGTACCTCTCAATAGCCAGACTAAGCAGCCTTAAC AIQRELNSTRYLSIARLSSLN -1.715 0.000313 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23916 GYTPESDLPKQYLMQFLFAY 20 SLAY-screened peptide P2266 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTATACGCCCGAGAGTGACCTCCCGAAGCAGTATCTTATGCAGTTCCTTTTTGCCTATTAA GYTPESDLPKQYLMQFLFAY* -1.715 0.030238 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23917 YTPPYSYLLMKTHRCIPSLI 20 SLAY-screened peptide P2267 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACGCCCCCCTACAGTTATCTTCTTATGAAGACTCACCGTTGTATTCCCTCTCTCATCTAA YTPPYSYLLMKTHRCIPSLI* -1.715 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23918 RSRSRGCFLGCTLDASPNAS 20 SLAY-screened peptide P2268 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTAGCAGGAGCCGCGGCTGCTTCCTCGGTTGTACTCTTGATGCGAGCCCGAATGCCTCGTAA RSRSRGCFLGCTLDASPNAS* -1.714 0.00569 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23919 LPTPSDYILPRGPPCRIKPS 20 SLAY-screened peptide P2269 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCCACTCCCAGTGATTATATTCTTCCCCGCGGCCCTCCCTGCCGTATTAAGCCGTCGTAA LPTPSDYILPRGPPCRIKPS* -1.714 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23920 STHRMCPFRNPYLQLTAYFK 20 SLAY-screened peptide P2270 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCACGCACCGCATGTGTCCGTTCAGGAACCCTTACTTGCAGCTGACGGCGTATTTCAAGTAA STHRMCPFRNPYLQLTAYFK* -1.714 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23921 ITFHYHWYF 9 SLAY-screened peptide P2271 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTACTTTCCATTACCACTGGTACTTCTAGAGGTCGCACCATACCTTTAAGCACGGTTTTTAA ITFHYHWYF*RSHHTFKHGF* -1.714 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23922 SPCRRLRLRRSPIAIDFYSV 20 SLAY-screened peptide P2272 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCCTTGCCGGCGTCTGCGCCTGCGTCGTAGTCCCATCGCTATCGATTTCTATAGCGTGTAA SPCRRLRLRRSPIAIDFYSV* -1.713 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23923 THVPIDISSPTFTVVLLLLM 20 SLAY-screened peptide P2273 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCATGTTCCCATCGACATTTCGTCGCCGACTTTTACTGTGGTTCTCTTGCTGCTTATGTAA THVPIDISSPTFTVVLLLLM* -1.713 4.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23924 SQPFVDAPGSKFQTHDIMRN 20 SLAY-screened peptide P2274 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCAGCCGTTTGTGGACGCGCCCGGTTCGAAGTTTCAGACGCATGATATCATGAGGAACTAA SQPFVDAPGSKFQTHDIMRN* -1.713 0.000605 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23925 LCRATPPQSATDAAVYFNHR 20 SLAY-screened peptide P2275 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTGCCGTGCGACCCCCCCCCAGTCGGCTACTGACGCTGCCGTGTATTTTAACCATCGGTAA LCRATPPQSATDAAVYFNHR* -1.713 0.001947 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23926 QGYS 4 SLAY-screened peptide P2276 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGGCTACAGTTAGGCTCCGCCGGTCCATGATACTACCAATGCGGCTCCTAACCATGTCTAA QGYS*APPVHDTTNAAPNHV* -1.713 6.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23927 SRRIDYESNSEEAQVITT 18 SLAY-screened peptide P2277 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCGCCGTATCGATTATGAGAGCAACTCTGAGGAGGCCCAGGTGATTACTACTTAGTCCTAA SRRIDYESNSEEAQVITT*S* -1.713 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23928 LLEYGYDKLRLGAGLYSPIH 20 SLAY-screened peptide P2278 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCTCGAGTATGGTTATGATAAGCTGCGCTTGGGTGCCGGGCTCTACAGCCCTATCCATTAA LLEYGYDKLRLGAGLYSPIH* -1.713 4.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23929 RYNCNSDCYTTVYHDSFTDS 20 SLAY-screened peptide P2279 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTATAATTGCAACTCGGACTGCTATACTACTGTCTATCATGATAGTTTCACCGACTCCTAA RYNCNSDCYTTVYHDSFTDS* -1.712 5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23930 SVADNPPWIAPICAPFAFSIN 21 SLAY-screened peptide P2280 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGTCGCTGACAACCCTCCTTGGATCGCGCCCATCTGCGCACCCTTTGCGTTTTCGATTAAC SVADNPPWIAPICAPFAFSIN -1.712 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23931 LRKLILWIVATRWPFVRRSFN 21 SLAY-screened peptide P2281 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCGTAAGCTTATCCTGTGGATAGTTGCCACGCGCTGGCCATTTGTTCGCAGAAGCTTTAAC LRKLILWIVATRWPFVRRSFN -1.712 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23932 LPPALRMQVFIPITSVITNPN 21 SLAY-screened peptide P2282 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCCCCGGCGCTCCGCATGCAGGTTTTTATTCCCATCACCAGTGTTATCACTAATCCTAAC LPPALRMQVFIPITSVITNPN -1.712 0.000602 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23933 PSQAPNTRVDSASCPGRAKP 20 SLAY-screened peptide P2283 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTCAGGCCCCTAATACTCGTGTTGACTCTGCGAGTTGTCCGGGGCGTGCTAAGCCTTAA PSQAPNTRVDSASCPGRAKP* -1.712 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23934 LTSTSINRVRLSRASMTFLM 20 SLAY-screened peptide P2284 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACTAGTACCAGTATCAATCGTGTGCGTCTTTCCCGTGCTTCTATGACTTTTTTGATGTAA LTSTSINRVRLSRASMTFLM* -1.711 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23935 SSRPAAIFQPRTYFNSLALS 20 SLAY-screened peptide P2285 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTCTCGCCCCGCCGCTATTTTTCAGCCGCGTACCTACTTCAACAGCCTCGCGTTGTCCTAA SSRPAAIFQPRTYFNSLALS* -1.711 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23936 DAHQNGVTLILCVPQHNSIA 20 SLAY-screened peptide P2286 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGCTCATCAGAACGGCGTCACGCTCATTCTGTGCGTGCCGCAGCATAATTCGATCGCTTAA DAHQNGVTLILCVPQHNSIA* -1.711 0.021184 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23937 PTSSYWPVRT 10 SLAY-screened peptide P2287 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACCTCTAGTTATTGGCCTGTTAGGACGTAGAGCCTGCTTTGCTAGCTCCTCTGCCCCTAA PTSSYWPVRT*SLLC*LLCP* -1.711 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23938 MSSVDCHHNKYPPVIACIPS 20 SLAY-screened peptide P2288 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTCTAGTGTTGATTGCCACCACAACAAGTACCCTCCGGTCATTGCTTGCATTCCCAGCTAA MSSVDCHHNKYPPVIACIPS* -1.711 0.014549 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23939 LLGYILSILYDLIFLWSTCL 20 SLAY-screened peptide P2289 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGCTCGGGTACATTCTTTCGATCTTGTACGATCTTATTTTCCTCTGGAGCACCTGTCTCTAA LLGYILSILYDLIFLWSTCL* -1.711 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23940 HIKETPPIPSVFLLGDHALI 20 SLAY-screened peptide P2290 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATTAAGGAGACGCCGCCCATTCCGTCGGTTTTCCTTCTCGGCGACCATGCTCTTATCTAA HIKETPPIPSVFLLGDHALI* -1.711 0.013553 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23941 TIVAPSVPPRRLMILPPHVA 20 SLAY-screened peptide P2291 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCATCGTCGCCCCCTCGGTGCCGCCTAGGCGCTTGATGATCTTGCCCCCTCACGTCGCGTAA TIVAPSVPPRRLMILPPHVA* -1.71 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23942 NNSTWDHPSHNIVGVLIFMFT 21 SLAY-screened peptide P2292 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATAATTCTACTTGGGATCACCCCTCTCATAATATTGTCGGTGTTTTGATTTTTATGTTTACT NNSTWDHPSHNIVGVLIFMFT -1.71 0.00543 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23943 GYYCYRAALRVPASSAYPFH 20 SLAY-screened peptide P2293 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGTACTATTGCTATCGGGCCGCCCTTCGGGTGCCGGCGTCTAGTGCCTACCCTTTCCATTAA GYYCYRAALRVPASSAYPFH* -1.71 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23944 NSIALSGHEWTQAGRTGNLA 20 SLAY-screened peptide P2294 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTCTATCGCTCTCTCTGGTCACGAGTGGACGCAGGCGGGCCGCACTGGGAATCTCGCGTAA NSIALSGHEWTQAGRTGNLA* -1.709 4.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23945 IHDSGRKSNSESLAPCYYNV 20 SLAY-screened peptide P2295 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCACGACAGTGGCCGGAAGAGTAACTCTGAGAGTCTGGCTCCTTGTTACTATAACGTCTAA IHDSGRKSNSESLAPCYYNV* -1.709 0.005877 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23946 SHSYRSLPCFTT 12 SLAY-screened peptide P2296 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCACTCGTACCGGAGTTTGCCGTGTTTCACCACCTAGCCTATTCGCGCTCTCACGGTTTAA SHSYRSLPCFTT*PIRALTV* -1.709 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23947 CRISCVFLPSRPTYLRMPLL 20 SLAY-screened peptide P2297 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAGGATCTCTTGTGTTTTCCTCCCCAGCCGCCCCACGTATTTGCGTATGCCCTTGCTTTAA CRISCVFLPSRPTYLRMPLL* -1.709 6e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23948 PVCATAARTPNLYHLADPST 20 SLAY-screened peptide P2298 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGTTTGCGCCACTGCCGCTCGTACGCCTAATTTGTATCACCTCGCGGACCCTAGCACCTAA PVCATAARTPNLYHLADPST* -1.708 0.000749 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23949 AQPYT 5 SLAY-screened peptide P2299 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCAGCCTTACACCTAGGATACTTCGGATAAGCAGAACTCTAAGAATTACCGCCTGCGCTAA AQPYT*DTSDKQNSKNYRLR* -1.708 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23950 LIYGYFLRLYCSPMSSMI 18 SLAY-screened peptide P2300 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCATTTATGGGTATTTCCTTCGGCTGTATTGCTCCCCGATGTCCTCCATGATTTAGGTGTAA LIYGYFLRLYCSPMSSMI*V* -1.708 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23951 AQVRLARISYTSPLPSLPRGN 21 SLAY-screened peptide P2301 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCAGGTTAGGCTTGCTAGGATCTCTTACACCAGCCCGCTACCTTCATTACCACGCGGTAAC AQVRLARISYTSPLPSLPRGN -1.707 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23952 PDNEQLTNCTNHSLFAKFTA 20 SLAY-screened peptide P2302 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGATAACGAGCAGCTCACTAACTGCACTAATCACAGCCTTTTTGCCAAGTTCACTGCCTAA PDNEQLTNCTNHSLFAKFTA* -1.707 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23953 LHPPNITPSVDSNY 14 SLAY-screened peptide P2303 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCACCCCCCCAACATCACGCCCTCCGTGGACTCTAATTATTAGAACTTGGATGTTTTTTAA LHPPNITPSVDSNY*NLDVF* -1.707 0.000899 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23954 VAPAGPLPL 9 SLAY-screened peptide P2304 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGCTCCTGCTGGTCCTCTGCCGTTGTAGTAGGGTTTTTTTCTTTATCCTCCTATCGGTTAA VAPAGPLPL**GFFLYPPIG* -1.707 0.000156 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23955 PIEYLLVSLNHHYLSHA 17 SLAY-screened peptide P2305 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTATCGAGTATTTGCTGGTCAGCCTGAATCACCATTATTTGAGTCATGCTTAGGAGCTTTAA PIEYLLVSLNHHYLSHA*EL* -1.707 0.002435 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23956 SHNITSSVCYHLYWDFSSLR 20 SLAY-screened peptide P2306 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCATAATATCACTAGTAGCGTTTGTTACCATCTTTACTGGGACTTCTCTAGCCTCCGGTAA SHNITSSVCYHLYWDFSSLR* -1.706 0.007143 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23957 LRVGFFSIHSTRRKRHR 17 SLAY-screened peptide P2307 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCGCGTCGGGTTCTTTAGTATTCACTCCACGAGGCGCAAGCGGCATCGGTAGTATAGCTAA LRVGFFSIHSTRRKRHR*YS* -1.706 0.001085 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23958 PPYRSSVSRFTN 12 SLAY-screened peptide P2308 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCTTACCGTTCGAGCGTTTCTCGCTTCACCAATTAGGCGTCTTTTTTGGATGCTGACTAA PPYRSSVSRFTN*ASFLDAD* -1.706 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23959 GFSTMPVPLCIFPSPWWTSI 20 SLAY-screened peptide P2309 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTTTTTCCACTATGCCCGTGCCTTTGTGTATCTTTCCTTCTCCTTGGTGGACCAGCATCTAA GFSTMPVPLCIFPSPWWTSI* -1.706 0.021577 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23960 HIYTDGPDPHNVYCNSICRH 20 SLAY-screened peptide P2310 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATCTATACTGACGGCCCGGATCCTCACAACGTTTATTGTAACAGCATTTGTCGCCACTAA HIYTDGPDPHNVYCNSICRH* -1.705 0.000267 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23961 VGPALPRSLPIGIYVTPLSS 20 SLAY-screened peptide P2311 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGGCCCGGCCCTTCCCCGCTCGCTTCCGATCGGTATCTATGTCACGCCTTTGTCGTCCTAA VGPALPRSLPIGIYVTPLSS* -1.705 0.02888 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23962 LSCSSDSRNNGLSCTY 16 SLAY-screened peptide P2312 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTCCTGCTCTTCTGATAGCCGCAACAACGGCCTGAGCTGCACTTACTAGCTTCGCTGCTAA LSCSSDSRNNGLSCTY*LRC* -1.705 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23963 PCRKNFSLRCFVSNLGDNML 20 SLAY-screened peptide P2313 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGCCGTAAGAATTTTTCCTTGCGGTGTTTCGTCAGTAATCTGGGCGACAACATGCTCTAA PCRKNFSLRCFVSNLGDNML* -1.705 0.002989 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23964 PLPVRTMSFTVPRSCTSIPS 20 SLAY-screened peptide P2314 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTTCCCGTTAGGACCATGAGTTTCACGGTGCCTCGCTCTTGCACTTCCATCCCGTCCTAA PLPVRTMSFTVPRSCTSIPS* -1.705 0.005139 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23965 TSYLRCSYCAGGMYGLRRSD 20 SLAY-screened peptide P2315 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTCTTACCTCCGTTGTTCTTATTGCGCCGGTGGTATGTATGGCCTGCGCCGGTCCGACTAA TSYLRCSYCAGGMYGLRRSD* -1.704 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23966 SSTGSSNRATYAYTSATHKP 20 SLAY-screened peptide P2316 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTCTACTGGTAGCTCGAACAGGGCCACGTATGCTTACACGTCTGCTACTCATAAGCCCTAA SSTGSSNRATYAYTSATHKP* -1.704 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23967 RD 2 SLAY-screened peptide P2317 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGATTAGTGTTATCGTTTGGACCATGAGTCCCCTGGTCGCAACTACTTTTAGCGTCCGTAA RD*CYRLDHESPGRNYF*RP* -1.704 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23968 CMRVLVLFRWRRSLRDL 17 SLAY-screened peptide P2318 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTATGAGGGTCCTGGTTCTGTTCAGGTGGCGCCGGAGTCTCCGTGATCTGTAGACCATGTAA CMRVLVLFRWRRSLRDL*TM* -1.704 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23969 QTNLSMDSHYSIAQPLCHSL 20 SLAY-screened peptide P2319 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGACCAATCTGAGTATGGACTCCCATTATTCTATTGCTCAGCCCCTCTGCCATTCCCTTTAA QTNLSMDSHYSIAQPLCHSL* -1.704 0.005167 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23970 CNTIRDKWVQMLYAMPTDYL 20 SLAY-screened peptide P2320 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAACACTATTCGCGACAAGTGGGTCCAGATGCTCTATGCGATGCCGACTGACTATCTTTAA CNTIRDKWVQMLYAMPTDYL* -1.703 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23971 NDPRLPSYYRHFLSLGFTTD 20 SLAY-screened peptide P2321 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGATCCGCGCTTGCCGAGCTACTATCGGCATTTCTTGTCGCTGGGTTTCACCACTGATTAA NDPRLPSYYRHFLSLGFTTD* -1.703 0.023868 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23972 SGRNGRTHGHHGTNRLLVRD 20 SLAY-screened peptide P2322 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGGGCGCAACGGTCGGACCCATGGGCATCATGGCACCAACCGGCTCTTGGTCCGTGATTAA SGRNGRTHGHHGTNRLLVRD* -1.703 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23973 ATPRFCNGVGCCWSSWILL 19 SLAY-screened peptide P2323 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTACCCCTCGTTTTTGTAATGGCGTTGGTTGCTGTTGGTCGAGCTGGATCCTTCTGTAGTAA ATPRFCNGVGCCWSSWILL** -1.703 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23974 RNVVGPYRCRACNALRINNT 20 SLAY-screened peptide P2324 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAATGTCGTCGGCCCTTATCGCTGTAGGGCGTGCAACGCTCTTCGCATCAACAATACGTAA RNVVGPYRCRACNALRINNT* -1.703 9.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23975 LQLTAFLKLVYRLISTYASP 20 SLAY-screened peptide P2325 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCAGCTTACTGCCTTTTTGAAGCTTGTTTACCGTCTTATTTCGACCTACGCTTCCCCTTAA LQLTAFLKLVYRLISTYASP* -1.702 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23976 RPPLPSSVLSFQAPHIYTLS 20 SLAY-screened peptide P2326 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCGCCCCTTCCGTCCTCGGTTCTTAGCTTCCAGGCCCCTCATATCTACACCCTTTCCTAA RPPLPSSVLSFQAPHIYTLS* -1.702 4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23977 RVHDFPWGSAGLSYNVSPITN 21 SLAY-screened peptide P2327 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGTTCACGATTTTCCTTGGGGCTCGGCCGGTCTCAGTTATAACGTGTCGCCTATAACTAAC RVHDFPWGSAGLSYNVSPITN -1.701 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23978 CRGAATPMYLNRSRRHYAGL 20 SLAY-screened peptide P2328 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGGGGGGCTGCGACCCCCATGTACTTGAACCGCTCGCGCCGTCATTATGCGGGCTTGTAA CRGAATPMYLNRSRRHYAGL* -1.701 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23979 LDLHLSPIFAPLCYSFLGSL 20 SLAY-screened peptide P2329 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGACCTTCACCTTTCGCCGATTTTCGCTCCTCTTTGCTATTCTTTCCTGGGGAGTCTTTAA LDLHLSPIFAPLCYSFLGSL* -1.701 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23980 IPFIYF 6 SLAY-screened peptide P2330 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCCCTTCATCTACTTTTAGGATACGCACACGGGCGTGATCGACAAGGCTTAGTAGGCTTAA IPFIYF*DTHTGVIDKA**A* -1.701 0.010875 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23981 AQPPADSRPAPNLFL 15 SLAY-screened peptide P2331 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCAGCCGCCCGCTGATTCTCGTCCGGCTCCGAACCTTTTCCTGTAGAGTTCCCTCTTAGTA AQPPADSRPAPNLFL*SSLLV -1.701 0.016131 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23982 HGNNTTRTSFKLSRLTTFFV 20 SLAY-screened peptide P2332 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGGTAACAACACCACGCGCACCAGCTTCAAGTTGTCCCGTCTCACGACTTTCTTCGTCTAA HGNNTTRTSFKLSRLTTFFV* -1.701 0.020868 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23983 RILRGRARHFGNAKRPLTSR 20 SLAY-screened peptide P2333 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGATCCTGCGGGGCCGCGCCCGCCACTTTGGCAACGCTAAGCGGCCCCTGACCTCTCGTTAA RILRGRARHFGNAKRPLTSR* -1.701 7.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23984 IHPHLLHCKNLFTRYNKYTH 20 SLAY-screened peptide P2334 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCACCCCCATCTGCTCCATTGTAAGAACCTTTTTACGCGCTATAACAAGTATACCCATTAA IHPHLLHCKNLFTRYNKYTH* -1.701 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23985 YYHTNHAYCDSFKITYEWLP 20 SLAY-screened peptide P2335 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTACCATACCAACCACGCCTACTGTGACTCTTTTAAGATCACCTATGAGTGGCTCCCGTAA YYHTNHAYCDSFKITYEWLP* -1.701 0.029454 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23986 RSRRLVFRLINLFCPMCFSS 20 SLAY-screened peptide P2336 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTCCCGCCGCCTGGTGTTCCGTCTGATTAATCTCTTTTGCCCTATGTGTTTTTCCTCCTAA RSRRLVFRLINLFCPMCFSS* -1.7 0.000189 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23987 PPNHQWRQMTTYSCRTSRAN 20 SLAY-screened peptide P2337 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCAACCACCAGTGGCGTCAGATGACTACTTACTCGTGTCGGACCTCTCGGGCTAATTAA PPNHQWRQMTTYSCRTSRAN* -1.7 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23988 GPAYTIHSVLSTSPTRLWSP 20 SLAY-screened peptide P2338 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCCCGCTTATACGATTCACTCGGTCCTTAGCACTTCTCCCACCAGGTTGTGGAGTCCGTAA GPAYTIHSVLSTSPTRLWSP* -1.7 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23989 SPKQPVASSCRPRRALPEELT 21 SLAY-screened peptide P2339 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCCCAAGCAGCCCGTTGCCAGTAGTTGCCGTCCGCGTCGCGCGCTCCCTGAAGAGCTAACT SPKQPVASSCRPRRALPEELT -1.7 7.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23990 PGWNNTKRYRKRLPLYRCMN 20 SLAY-screened peptide P2340 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGGTTGGAATAACACTAAGCGTTACCGCAAGCGTCTTCCCCTTTACCGCTGCATGAATTAA PGWNNTKRYRKRLPLYRCMN* -1.7 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23991 LYKVYKYLFPISLLKNMTPF 20 SLAY-screened peptide P2341 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTATAAGGTCTACAAGTATTTGTTTCCCATTTCGTTGTTGAAGAACATGACTCCGTTTTAA LYKVYKYLFPISLLKNMTPF* -1.699 0.00013 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23992 FHALWLVATGYFFNRVKMLS 20 SLAY-screened peptide P2342 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCACGCGCTCTGGCTCGTTGCCACCGGCTATTTTTTTAATAGGGTTAAGATGCTTTCTTAA FHALWLVATGYFFNRVKMLS* -1.699 2.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23993 RRVMYNSDTYSTRGQIKYH 19 SLAY-screened peptide P2343 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCGCGTTATGTATAATTCCGACACGTATAGCACCAGGGGTCAGATTAAGTATCACTAGTAA RRVMYNSDTYSTRGQIKYH** -1.699 0.002533 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23994 VVDAPRDAPRVLDI 14 SLAY-screened peptide P2344 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCGTTGATGCGCCTCGGGATGCCCCGCGGGTTCTTGACATTTAGCGGCTTCTGGCTTGGTAA VVDAPRDAPRVLDI*RLLAW* -1.699 0.012605 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23995 PLIPWRANIPTCPCWFCHYL 20 SLAY-screened peptide P2345 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCATTCCGTGGCGTGCCAATATCCCGACGTGTCCTTGCTGGTTCTGCCACTACCTTTAA PLIPWRANIPTCPCWFCHYL* -1.699 0.017275 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23996 FVRDLVTSFRRDVFTRCTKG 20 SLAY-screened peptide P2346 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGTCCGGGACCTTGTCACGTCCTTTAGGCGTGATGTGTTTACTCGTTGCACCAAGGGCTAA FVRDLVTSFRRDVFTRCTKG* -1.698 0.017482 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23997 SVKGHCKFFLNCQRASYSPY 20 SLAY-screened peptide P2347 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGTTAAGGGTCATTGCAAGTTTTTTCTGAACTGTCAGAGGGCGAGCTATTCGCCCTACTAA SVKGHCKFFLNCQRASYSPY* -1.698 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23998 PPPYSLGSSAVYDSIPIYFL 20 SLAY-screened peptide P2348 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTCCTTACAGCCTGGGTTCCTCGGCTGTTTACGATAGCATCCCGATTTATTTTTTGTAA PPPYSLGSSAVYDSIPIYFL* -1.698 6.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP23999 TACDPYNIHAYHL 13 SLAY-screened peptide P2349 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGCGTGTGATCCGTACAATATCCACGCGTATCATCTGTAGTTTAACTAGCGTTGGTATTAA TACDPYNIHAYHL*FN*RWY* -1.698 0.000242 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24000 RRMQCPYCLLAISANSYTLN 20 SLAY-screened peptide P2350 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCGCATGCAGTGCCCTTACTGCCTGTTGGCCATTTCCGCGAACTCCTACACTCTTAACTAA RRMQCPYCLLAISANSYTLN* -1.697 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24001 FIFYSV 6 SLAY-screened peptide P2351 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCATCTTTTACTCCGTTTAGTATTTTAGCCGGTTTCTTAAGATCCTTCCCCTTGATTTCTAA FIFYSV*YFSRFLKILPLDF* -1.697 0.000714 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24002 LFIAFLSILHIQLTFLITRR 20 SLAY-screened peptide P2352 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTTCATTGCTTTCTTGAGTATTTTGCACATTCAGCTGACCTTTTTGATTACCCGCCGTTAA LFIAFLSILHIQLTFLITRR* -1.697 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24003 AVNRYICRMPCRIRRPST 18 SLAY-screened peptide P2353 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGTCAACCGTTACATCTGCCGCATGCCGTGTCGGATCAGGCGGCCTTCGACTTAGACTTAC AVNRYICRMPCRIRRPST*TY -1.697 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24004 WPVDSTLAFLSVFFLH 16 SLAY-screened peptide P2354 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGGCCCGTCGATAGTACGCTCGCGTTCCTCTCGGTCTTCTTTCTGCATTAGTACGTTTTCTAA WPVDSTLAFLSVFFLH*YVF* -1.696 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24005 DTTPVCNMCPSPQESYCRSQ 20 SLAY-screened peptide P2355 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATACGACCCCCGTGTGTAACATGTGCCCGTCGCCGCAGGAGTCTTACTGTAGGAGTCAGTAA DTTPVCNMCPSPQESYCRSQ* -1.696 0.000121 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24006 DLRSGERHTRCIEDNFLITT 20 SLAY-screened peptide P2356 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCTTCGTTCCGGCGAGAGGCACACTCGCTGTATTGAGGATAATTTCCTCATTACGACCTAA DLRSGERHTRCIEDNFLITT* -1.696 0.000164 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24007 SEIFNNYTREKHCCPNSLTVY 21 SLAY-screened peptide P2357 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGAGATTTTCAATAATTATACTCGTGAGAAGCACTGCTGTCCGAATTCTCTCACTGTGTAC SEIFNNYTREKHCCPNSLTVY -1.696 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24008 MLGAVPTMQPAINTFGA 17 SLAY-screened peptide P2358 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCTCGGGGCGGTCCCCACCATGCAGCCGGCGATCAACACCTTCGGGGCCTAGTGTGTTTAA MLGAVPTMQPAINTFGA*CV* -1.696 0.021254 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24009 TITPRGFHRISTIGNCCRHA 20 SLAY-screened peptide P2359 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCATCACCCCTCGGGGCTTTCATAGGATCAGTACTATCGGTAACTGCTGCCGCCACGCCTAA TITPRGFHRISTIGNCCRHA* -1.695 0.025356 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24010 RLHDDMPKITTAYKGFCHSI 20 SLAY-screened peptide P2360 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCTTCACGACGATATGCCCAAGATCACTACTGCCTATAAGGGGTTTTGCCACTCGATTTAA RLHDDMPKITTAYKGFCHSI* -1.695 0.000247 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24011 KYHPDLRIVYICFSLYCSTY 20 SLAY-screened peptide P2361 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGTACCACCCGGATTTGCGTATTGTGTATATCTGTTTTTCCCTCTACTGTAGCACTTATTAA KYHPDLRIVYICFSLYCSTY* -1.695 0.003804 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24012 ILRRRRTRHHSYVVITLSFD 20 SLAY-screened peptide P2362 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCTGCGTCGTCGTCGGACGAGGCATCACTCGTATGTTGTCATTACTCTGTCGTTTGACTAA ILRRRRTRHHSYVVITLSFD* -1.695 0.001392 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24013 SYRFKAEACFLDFSEDNSFC 20 SLAY-screened peptide P2363 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTATAGGTTCAAGGCGGAGGCCTGTTTCCTTGATTTTTCCGAGGATAATTCTTTCTGTTAA SYRFKAEACFLDFSEDNSFC* -1.695 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24014 PIHTTYRTRRHRHSRMYRIP 20 SLAY-screened peptide P2364 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGATCCACACGACTTACCGGACTCGTCGGCACCGGCACTCCCGTATGTACCGTATTCCGTAA PIHTTYRTRRHRHSRMYRIP* -1.694 0.007332 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24015 VTQFPESIYALPDLNHFKYI 20 SLAY-screened peptide P2365 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCACTCAGTTCCCGGAGTCTATCTATGCCCTCCCCGACCTCAACCATTTTAAGTATATTTAA VTQFPESIYALPDLNHFKYI* -1.694 5.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24016 RHSYGSAYSKPLVYIPPGTT 20 SLAY-screened peptide P2366 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCATTCTTATGGGAGTGCGTACTCTAAGCCTTTGGTTTATATTCCGCCGGGCACCACTTAA RHSYGSAYSKPLVYIPPGTT* -1.694 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24017 NYMYSYTFP 9 SLAY-screened peptide P2367 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTACATGTACAGCTACACGTTCCCATGATCTGTACCTGGTTGTGTGGTACCAAGGACTAAC NYMYSYTFP*SVPGCVVPRTN -1.694 0.000637 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24018 APMEGQ 6 SLAY-screened peptide P2368 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCCCATGGAGGGCCAGTAGATCCCCATCCACAACACCGTTAATCATTACAACGTCAGGTAA APMEGQ*IPIHNTVNHYNVR* -1.693 0.028063 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24019 SHFRAYLMLL 10 SLAY-screened peptide P2369 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCATTTTCGTGCGTATCTTATGCTGCTCTAGTTTGCTGGGTCGATGTCCGACAACCGCTAA SHFRAYLMLL*FAGSMSDNR* -1.693 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24020 GLRTTHEVDHALSSHISGHH 20 SLAY-screened peptide P2370 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGTTGCGCACCACGCACGAGGTCGACCATGCGCTTAGCTCGCATATCTCTGGTCATCACTAA GLRTTHEVDHALSSHISGHH* -1.693 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24021 QDAIDHPFHAYRSKINTYLM 20 SLAY-screened peptide P2371 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGATGCTATCGACCATCCGTTCCATGCCTACCGTAGTAAGATTAACACTTATCTGATGTAA QDAIDHPFHAYRSKINTYLM* -1.693 0.040637 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24022 SQQATIRVDYLRFLYIQHYL 20 SLAY-screened peptide P2372 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCAGCAGGCGACTATCCGCGTGGACTATCTCAGGTTCCTTTATATTCAGCATTATCTGTAA SQQATIRVDYLRFLYIQHYL* -1.693 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24023 NIFYDRDNSCPRETTHFDYR 20 SLAY-screened peptide P2373 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATATCTTTTACGATCGCGATAACTCTTGTCCCCGGGAGACCACTCACTTTGACTACCGTTAA NIFYDRDNSCPRETTHFDYR* -1.693 0.00156 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24024 LHVYYPFLL 9 SLAY-screened peptide P2374 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCACGTGTACTACCCTTTTCTCCTGTAGACGGTTAATCCCTGTCCTTGCGAGTAGCACTAA LHVYYPFLL*TVNPCPCE*H* -1.693 0.001921 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24025 AHLYPTALYN 10 SLAY-screened peptide P2375 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCATCTTTACCCGACTGCGCTGTATAATTAGGTGACTGCCCCTATCTTGGCTCCGCGTTAA AHLYPTALYN*VTAPILAPR* -1.693 0.018557 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24026 DSFMDAWSGNYFLTPVRRSRN 21 SLAY-screened peptide P2376 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTCTTTTATGGATGCTTGGTCGGGTAACTATTTTTTGACACCAGTTCGCAGGTCTCGTAAC DSFMDAWSGNYFLTPVRRSRN -1.693 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24027 SANRIRIHFLMITNITHQDD 20 SLAY-screened peptide P2377 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGCTAACCGCATCAGGATCCATTTTCTTATGATCACTAACATCACTCACCAGGATGACTAA SANRIRIHFLMITNITHQDD* -1.692 0.000517 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24028 HPHYYEYCSTATSPTNNIGS 20 SLAY-screened peptide P2378 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCCCATTATTATGAGTACTGCTCCACGGCCACCAGTCCCACTAATAATATCGGTAGCTAA HPHYYEYCSTATSPTNNIGS* -1.692 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24029 PALIQSAQHVLSDTFCLLCC 20 SLAY-screened peptide P2379 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGCCCTTATCCAGAGTGCTCAGCATGTTCTGTCTGATACCTTTTGCCTCCTCTGTTGTTAA PALIQSAQHVLSDTFCLLCC* -1.692 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24030 SL 2 SLAY-screened peptide P2380 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTTGTAGTTTTCGTGTCTTGACCGTTGTATTGATCTTCACATTTGTGTTCATTATACCTAA SL*FSCLDRCIDLHICVHYT* -1.692 0.001525 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24031 IPDVLLDSWNRYGRFIL 17 SLAY-screened peptide P2381 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCCTGACGTCCTCCTGGACTCCTGGAACAGGTACGGAAGGTTCATACTTTGACGCTCTAAC IPDVLLDSWNRYGRFIL*RSN -1.692 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24032 VPNAYPSQRSYHPPSLSSIT 20 SLAY-screened peptide P2382 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCCCAACGCCTATCCGAGTCAGAGGTCTTACCATCCCCCTAGCCTTAGCTCGATTACCTAA VPNAYPSQRSYHPPSLSSIT* -1.692 0.002021 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24033 PDVLD 5 SLAY-screened peptide P2383 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGATGTGCTGGACTAGCCGGTCGTCAGCCGTCTCATCTACAGGGACCGTCCGGACAACTAA PDVLD*PVVSRLIYRDRPDN* -1.691 0.020668 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24034 RRAGAQITLSVLFYPGCALV 20 SLAY-screened peptide P2384 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCGCGCCGGGGCTCAGATTACCCTTAGCGTGCTTTTTTATCCCGGCTGCGCTTTGGTTTAA RRAGAQITLSVLFYPGCALV* -1.69 6.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24035 LPSRNPYGWSTVAGPLIHII 20 SLAY-screened peptide P2385 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCCAGCCGGAATCCCTACGGTTGGTCCACTGTTGCTGGCCCTCTTATTCATATTATTTAA LPSRNPYGWSTVAGPLIHII* -1.69 0.023234 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24036 NACCVFAPAPCADRGALGCN 20 SLAY-screened peptide P2386 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGCCTGCTGTGTCTTTGCCCCGGCCCCCTGTGCTGACCGTGGTGCTTTGGGGTGTAATTAA NACCVFAPAPCADRGALGCN* -1.69 0.015092 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24037 VNSVTIVLVFSLFFRRGN 18 SLAY-screened peptide P2387 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTAATTCCGTAACAATCGTACTTGTCTTCTCATTGTTTTTTAGACGGGGTAACTGAGTAAGT VNSVTIVLVFSLFFRRGN*VS -1.69 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24038 ALSASIGDFSRNPLKFVSNN 20 SLAY-screened peptide P2388 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTCTCTCTGCCTCGATCGGTGACTTTTCGAGGAACCCTTTGAAGTTCGTCTCGAACAATTAA ALSASIGDFSRNPLKFVSNN* -1.69 0.001836 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24039 PLTCPLSCCLWAWAPRRSLV 20 SLAY-screened peptide P2389 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTACCTGCCCGCTCAGCTGCTGCCTCTGGGCTTGGGCCCCTCGTAGGAGTCTGGTTTAA PLTCPLSCCLWAWAPRRSLV* -1.69 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24040 YAIYSLDFNDGFSHVTPDWS 20 SLAY-screened peptide P2390 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCTATCTATTCGCTGGATTTTAATGACGGGTTCAGTCACGTTACTCCCGATTGGTCTTAA YAIYSLDFNDGFSHVTPDWS* -1.69 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24041 QALPAPNALTRATVIGVHFI 20 SLAY-screened peptide P2391 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGCGCTGCCCGCTCCCAACGCGCTTACGCGCGCGACTGTGATTGGCGTGCACTTCATTTAA QALPAPNALTRATVIGVHFI* -1.69 0.001147 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24042 VRVKAHINRLFLTYKHVYAD 20 SLAY-screened peptide P2392 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCAGGGTTAAGGCGCACATTAACCGTCTTTTTCTGACTTATAAGCATGTTTATGCCGACTAA VRVKAHINRLFLTYKHVYAD* -1.689 0.008145 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24043 CA 2 SLAY-screened peptide P2393 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGCGTAGAGCCAGACTGGAATTTGTCGCGCACGGGGCCTTATGGTAAGTAGCATAAGTAAC CA*SQTGICRARGLMVSSISN -1.689 0.00087 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24044 SHWNNFHFTFTCEVGHPKLC 20 SLAY-screened peptide P2394 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCACTGGAATAATTTTCACTTCACGTTTACCTGTGAGGTTGGGCACCCTAAGCTGTGTTAA SHWNNFHFTFTCEVGHPKLC* -1.689 0.017499 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24045 ELPLVHALVFWLRITQSPVV 20 SLAY-screened peptide P2395 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCTGCCTCTGGTCCACGCCCTGGTTTTTTGGCTGCGTATCACTCAGTCGCCCGTGGTTTAA ELPLVHALVFWLRITQSPVV* -1.689 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24046 VYPHPTPGHATNCDAPCRFV 20 SLAY-screened peptide P2396 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCTATCCTCACCCTACCCCCGGGCATGCTACCAATTGTGACGCGCCTTGTCGGTTCGTGTAA VYPHPTPGHATNCDAPCRFV* -1.688 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24047 AMTRILTISTSTVSG 15 SLAY-screened peptide P2397 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGATGACGCGTATTTTAACAATATCCACATCAACTGTAAGTGGCTGATTATTTATTATTAAC AMTRILTISTSTVSG*LFIIN -1.688 0.0022 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24048 SYQPIIRITSIPYTLSL 17 SLAY-screened peptide P2398 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTATCAGCCCATTATCCGCATTACGTCCATCCCCTACACCCTTTCCCTCTAGTGTCGTTAA SYQPIIRITSIPYTLSL*CR* -1.688 0.001819 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24049 NQTVGGPSRHHTAELLRIVD 20 SLAY-screened peptide P2399 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCAGACTGTGGGCGGCCCGTCCCGTCATCACACCGCTGAGCTCCTCAGGATCGTGGACTAA NQTVGGPSRHHTAELLRIVD* -1.688 0.046602 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24050 PCTPLNNSSRLYDLAPSSTA 20 SLAY-screened peptide P2400 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGTACGCCGCTTAATAACAGTAGCCGTCTGTACGATTTGGCTCCCAGCAGTACCGCGTAA PCTPLNNSSRLYDLAPSSTA* -1.688 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24051 SHASTCFMYWLSKFTTTLIR 20 SLAY-screened peptide P2401 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCACGCCTCGACTTGCTTTATGTACTGGCTGAGTAAGTTTACTACGACTCTTATTCGCTAA SHASTCFMYWLSKFTTTLIR* -1.688 0.007768 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24052 HPSSEAVYSSLCLITPATGS 20 SLAY-screened peptide P2402 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCCAGCTCGGAGGCCGTTTATTCTTCCCTCTGTCTTATTACCCCCGCGACCGGCTCTTAA HPSSEAVYSSLCLITPATGS* -1.687 0.000186 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24053 GDHFTHAPATSQHICPMFAD 20 SLAY-screened peptide P2403 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTGACCATTTTACTCACGCCCCGGCGACCAGCCAGCATATCTGTCCTATGTTTGCTGACTAA GDHFTHAPATSQHICPMFAD* -1.687 0.024453 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24054 HPLSPNREPFCPHATWYKLP 20 SLAY-screened peptide P2404 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCTCTGAGTCCCAACCGTGAGCCTTTTTGTCCGCACGCTACGTGGTACAAGCTCCCCTAA HPLSPNREPFCPHATWYKLP* -1.687 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24055 PLNLSLHISSDPHICIDHVP 20 SLAY-screened peptide P2405 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCAACTTGTCCCTGCATATTAGTAGTGACCCGCACATTTGCATCGATCATGTTCCTTAA PLNLSLHISSDPHICIDHVP* -1.686 0.000357 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24056 DHGVVVVSFCRYPTCPANRT 20 SLAY-screened peptide P2406 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCACGGCGTGGTTGTCGTGTCCTTCTGTAGGTATCCTACTTGCCCTGCTAATCGTACCTAA DHGVVVVSFCRYPTCPANRT* -1.686 0.003789 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24057 RHAHMQTSTSPHH 13 SLAY-screened peptide P2407 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCACGCCCATATGCAGACCTCCACTAGCCCCCATCATTAGTGTCCTTTTTATGTCGGGTAA RHAHMQTSTSPHH*CPFYVG* -1.686 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24058 GLGVIYHMPGRCALLSCGAT 20 SLAY-screened peptide P2408 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCTGGGTGTTATCTACCATATGCCTGGCCGCTGCGCGCTTCTCTCGTGCGGGGCCACTTAA GLGVIYHMPGRCALLSCGAT* -1.686 0.006992 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24059 PRITAHDAMHS 11 SLAY-screened peptide P2409 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCGCATCACCGCGCACGATGCCATGCATAGCTAGCCCCCTATCATTTCTTTCGGCATTTAA PRITAHDAMHS*PPIISFGI* -1.686 3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24060 HNGGLPVFDRRFGPLPRYYA 20 SLAY-screened peptide P2410 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAACGGCGGGTTGCCGGTTTTCGACCGCAGGTTCGGCCCGCTGCCGCGTTATTATGCTTAA HNGGLPVFDRRFGPLPRYYA* -1.685 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24061 LNSRRDTIHVSFYVKYIHPP 20 SLAY-screened peptide P2411 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAACAGTCGGCGTGATACCATTCATGTCAGTTTTTACGTCAAGTACATCCATCCCCCGTAA LNSRRDTIHVSFYVKYIHPP* -1.685 0.003698 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24062 PYLTP 5 SLAY-screened peptide P2412 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTACCTTACGCCGTAGCTCTTTCATCTGATCAATTTGGTGGACTATAGCACGTTCAACTAA PYLTP*LFHLINLVDYSTFN* -1.685 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24063 ERWLSITLLTLTGPPGTIRAN 21 SLAY-screened peptide P2413 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAACGATGGCTATCCATCACTCTACTGACACTGACTGGGCCTCCCGGTACTATCAGAGCTAAC ERWLSITLLTLTGPPGTIRAN -1.685 0.001618 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24064 YYLSPSLTPVR 11 SLAY-screened peptide P2414 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTACTTGAGTCCCTCGCTCACTCCGGTTCGTTAGATTTATAGGGCTATGCACTCTTATTAA YYLSPSLTPVR*IYRAMHSY* -1.685 0.005885 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24065 LLPPMYGPYSDASVLPAKTP 20 SLAY-screened peptide P2415 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCTGCCCCCTATGTATGGTCCCTACTCGGACGCGAGCGTGTTGCCGGCTAAGACGCCCTAA LLPPMYGPYSDASVLPAKTP* -1.685 1.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24066 VLLTHAATFPESDVHVRLPN 20 SLAY-screened peptide P2416 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCTGCTTACCCACGCTGCCACGTTCCCGGAGAGCGATGTTCACGTGCGCCTGCCGAACTAA VLLTHAATFPESDVHVRLPN* -1.684 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24067 SSKHDRCSIRVVCFTDCNNY 20 SLAY-screened peptide P2417 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTCCAAGCACGATCGCTGCTCTATCCGCGTGGTCTGCTTCACGGATTGTAACAACTATTAA SSKHDRCSIRVVCFTDCNNY* -1.684 0.002402 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24068 GPWAIYKNGGDVPMVTSAPS 20 SLAY-screened peptide P2418 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCCTTGGGCCATTTATAAGAACGGCGGTGATGTCCCCATGGTGACTTCCGCTCCCTCCTAA GPWAIYKNGGDVPMVTSAPS* -1.684 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24069 RSTHYNMTLS 10 SLAY-screened peptide P2419 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTCGACGCACTACAACATGACTCTGTCCTAGACGGTTTATCCCCGTAAGTCTCCCTCTTAA RSTHYNMTLS*TVYPRKSPS* -1.684 0.000837 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24070 INVHVFFLSGKKSSRRDYDS 20 SLAY-screened peptide P2420 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAACGTTCACGTTTTTTTCCTTTCTGGTAAGAAGAGCTCTAGGCGCGATTATGACTCTTAA INVHVFFLSGKKSSRRDYDS* -1.684 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24071 YAPYKYSMVRAYNNLSASKK 20 SLAY-screened peptide P2421 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCCCCTTACAAGTACTCCATGGTTCGCGCGTATAATAACTTGTCCGCCTCGAAGAAGTAA YAPYKYSMVRAYNNLSASKK* -1.683 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24072 RFSRNHRSDFRKTAPSSTQY 20 SLAY-screened peptide P2422 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTTCTCTCGTAATCATCGCTCTGATTTTCGCAAGACTGCGCCCTCCAGTACTCAGTACTAA RFSRNHRSDFRKTAPSSTQY* -1.682 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24073 HNTPFHFPAGESTYRNYPDA 20 SLAY-screened peptide P2423 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAACACCCCCTTCCACTTTCCTGCGGGCGAGAGCACCTATCGTAACTATCCTGACGCGTAA HNTPFHFPAGESTYRNYPDA* -1.682 0.017059 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24074 RTGCINVTRTASTTSSLNVK 20 SLAY-screened peptide P2424 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGACGGGGTGTATTAATGTCACCCGGACTGCGTCCACTACGAGCAGCCTGAATGTCAAGTAA RTGCINVTRTASTTSSLNVK* -1.682 0.004405 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24075 TPSLTMTLYPMRKRILSRTP 20 SLAY-screened peptide P2425 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCCCAGCCTGACCATGACGCTGTATCCCATGCGGAAGCGCATCCTCTCTCGTACGCCGTAA TPSLTMTLYPMRKRILSRTP* -1.682 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24076 TGGSVHTMLHDPNYIGCANL 20 SLAY-screened peptide P2426 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGCGGCAGTGTCCACACGATGCTCCACGATCCCAACTACATTGGCTGCGCGAACCTGTAA TGGSVHTMLHDPNYIGCANL* -1.682 0.00173 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24077 CVFFALLETNPNGDHDPSSI 20 SLAY-screened peptide P2427 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGTCTTCTTCGCTCTCCTCGAGACTAATCCGAATGGTGACCACGACCCGTCTTCGATCTAA CVFFALLETNPNGDHDPSSI* -1.682 0.016679 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24078 PGIPIDSCYNCYFDIRPGST 20 SLAY-screened peptide P2428 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGGGGATTCCCATTGATAGCTGTTATAACTGCTATTTTGATATCCGGCCGGGTAGTACCTAA PGIPIDSCYNCYFDIRPGST* -1.682 0.000179 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24079 GRGIHRVPCTALSDPGYLVA 20 SLAY-screened peptide P2429 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTCGCGGCATCCATCGCGTTCCGTGTACCGCCTTGTCCGACCCTGGCTATCTCGTCGCTTAA GRGIHRVPCTALSDPGYLVA* -1.681 0.018335 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24080 DQDYGFLHDASSFYNHNYRP 20 SLAY-screened peptide P2430 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCAGGACTACGGTTTCCTCCACGACGCTTCGTCTTTTTACAACCACAACTACAGGCCTTAA DQDYGFLHDASSFYNHNYRP* -1.681 2.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24081 MVHLNNILEPASNTSHMAVN 20 SLAY-screened peptide P2431 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGGTGCATCTGAACAACATTCTCGAGCCGGCCTCTAACACTAGTCATATGGCCGTTAATTAA MVHLNNILEPASNTSHMAVN* -1.681 2.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24082 YPWSQSKPDS 10 SLAY-screened peptide P2432 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCCGTGGTCGCAGTCGAAGCCGGACAGCTAGAAGAATCCGAAGTATATGCGCCTCAGTTAA YPWSQSKPDS*KNPKYMRLS* -1.681 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24083 CDWPISTPQDNNIRCQRILCN 21 SLAY-screened peptide P2433 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGACTGGCCCATTAGTACCCCTCAGGATAACAATATTCGTTGTCAAAGAATATTATGTAAC CDWPISTPQDNNIRCQRILCN -1.68 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24084 HAMPDATILPSVTDYTTSYS 20 SLAY-screened peptide P2434 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGCTATGCCTGATGCTACTATTCTCCCTTCTGTGACGGATTACACTACGTCGTATAGCTAA HAMPDATILPSVTDYTTSYS* -1.68 0.026763 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24085 SILRHCSCNCLDQLIT 16 SLAY-screened peptide P2435 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGATCCTGCGCCACTGCAGCTGCAACTGTCTTGATCAGCTCATTACTTAGGACGTCACCTAA SILRHCSCNCLDQLIT*DVT* -1.68 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24086 SSCSVSTTAYLSYPPNRHIH 20 SLAY-screened peptide P2436 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAGCTGTAGTGTCAGTACGACCGCCTACCTGTCTTATCCGCCTAATAGGCATATCCATTAA SSCSVSTTAYLSYPPNRHIH* -1.68 0.001013 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24087 VCVPQAPEYRVFPLYNT 17 SLAY-screened peptide P2437 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTGTGTGCCTCAGGCTCCCGAGTATCGCGTGTTTCCGCTTTATAACACTTAGCTGGCGTAA VCVPQAPEYRVFPLYNT*LA* -1.68 0.007508 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24088 LRQTPPHFYFNLCYPHWRPP 20 SLAY-screened peptide P2438 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCGCCAGACGCCGCCCCACTTCTATTTCAACCTCTGCTATCCTCATTGGAGGCCGCCGTAA LRQTPPHFYFNLCYPHWRPP* -1.679 0.000164 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24089 SVTFS 5 SLAY-screened peptide P2439 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTGTGACTTTCTCCTAGGTTGGCTCCCCCGCGATCCATCGCTGGTTTCTCATCTCGAGTTAA SVTFS*VGSPAIHRWFLISS* -1.679 8.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24090 FPLFHNIHPTNLPVPREDFE 20 SLAY-screened peptide P2440 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCCCCTGTTTCATAACATTCATCCCACTAACCTTCCGGTGCCTCGGGAGGACTTTGAGTAA FPLFHNIHPTNLPVPREDFE* -1.679 3.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24091 PLSCPSHEPFICLRTLRFVR 20 SLAY-screened peptide P2441 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTGTCGTGCCCTTCGCACGAGCCCTTTATTTGCCTCCGCACGCTCCGCTTTGTTAGGTAA PLSCPSHEPFICLRTLRFVR* -1.679 0.004667 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24092 TSDSSST 7 SLAY-screened peptide P2442 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAGTGACTCTTCCTCGACCTAGCGGTTCAGTTGCATCTGTCTTGAGACTCGCCCGTCGTAA TSDSSST*RFSCICLETRPS* -1.678 0.008806 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24093 VNLT 4 SLAY-screened peptide P2443 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTAACCTTACTTAGCACCACACGCCTTTTCAGCACATTTACTATTCTTTTTCGTAGCATTAA VNLT*HHTPFQHIYYSFS*H* -1.678 0.008873 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24094 LPPIRDWRSVIVLPLHIATI 20 SLAY-screened peptide P2444 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCCCCCATCCGCGACTGGCGTTCCGTCATTGTCCTGCCTCTTCATATTGCGACGATCTAA LPPIRDWRSVIVLPLHIATI* -1.678 0.001827 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24095 PGYFPWNHRSPRLTESDTAQ 20 SLAY-screened peptide P2445 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGTTATTTTCCCTGGAACCATAGGTCGCCCCGTCTGACGGAGTCCGATACTGCTCAGTAA PGYFPWNHRSPRLTESDTAQ* -1.678 2.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24096 CNCCVHSPTYYPALLWAN 18 SLAY-screened peptide P2446 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAATTGCTGCGTTCATAGTCCCACCTATTATCCCGCTCTCCTTTGGGCGAACTAGACCTAA CNCCVHSPTYYPALLWAN*T* -1.678 0.02565 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24097 NNQDATYGSVRWLPVDSASL 20 SLAY-screened peptide P2447 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACAACCAGGATGCCACTTATGGCAGTGTTCGCTGGCTTCCTGTTGACTCGGCCTCGCTTTAA NNQDATYGSVRWLPVDSASL* -1.677 0.001445 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24098 VDLRVAQSKPLYRSSLMLHR 20 SLAY-screened peptide P2448 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGGATTTGCGCGTGGCTCAGTCTAAGCCGCTGTACAGGTCTTCGCTTATGTTGCATAGGTAA VDLRVAQSKPLYRSSLMLHR* -1.677 0.022009 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24099 SAGLSFRIVSFFHFRVRLRFN 21 SLAY-screened peptide P2449 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCGCTGGTCTGTCCTTTAGGATCGTTAGCTTTTTCCATTTTAGGGTTAGGCTAAGGTTTAAC SAGLSFRIVSFFHFRVRLRFN -1.676 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24100 AALKCLLSLPVYPQRAADSF 20 SLAY-screened peptide P2450 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGCCCTTAAGTGTCTCCTTAGTCTGCCGGTCTATCCGCAGAGGGCCGCTGACTCCTTCTAA AALKCLLSLPVYPQRAADSF* -1.676 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24101 RMCRKFKTWTHLHLRGRRTR 20 SLAY-screened peptide P2451 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCATGTGCCGTAAGTTTAAGACCTGGACGCATCTGCATCTCAGGGGCCGCCGGACCCGTTAA RMCRKFKTWTHLHLRGRRTR* -1.676 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24102 PSWT 4 SLAY-screened peptide P2452 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCGTGGACCTAGCGCCCCCGGTATTCTTGTACTAACCTGTTCTACTATCCCTGCTTTTAA PSWT*RPRYSCTNLFYYPCF* -1.676 0.00078 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24103 LLCMLCGPTPFHHCPASSLH 20 SLAY-screened peptide P2453 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTTGTGTATGCTGTGCGGCCCTACTCCTTTTCATCATTGCCCTGCCAGTTCGCTGCATTAA LLCMLCGPTPFHHCPASSLH* -1.675 3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24104 FSSHTPAPCMPPPNWRFAWV 20 SLAY-screened peptide P2454 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTAGTTCTCATACGCCGGCGCCCTGTATGCCCCCTCCGAACTGGAGGTTCGCGTGGGTGTAA FSSHTPAPCMPPPNWRFAWV* -1.674 0.001981 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24105 GPVSDSSGPFSHCIILAAPV 20 SLAY-screened peptide P2455 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGCCGGTCTCTGACAGTAGTGGGCCGTTCAGCCATTGCATCATCCTGGCTGCTCCTGTTTAA GPVSDSSGPFSHCIILAAPV* -1.674 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24106 LSPLPRHNISQTLRPPFLV 19 SLAY-screened peptide P2456 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTCCCCTTTGCCTCGGCATAATATCTCTCAGACGCTGCGCCCCCCCTTCCTGGTCTAGTAA LSPLPRHNISQTLRPPFLV** -1.674 0.006374 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24107 PVFIYSLKPMVPKKPNTRVL 20 SLAY-screened peptide P2457 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGTGTTTATCTACTCCCTCAAGCCTATGGTCCCGAAGAAGCCTAACACGCGTGTTTTGTAA PVFIYSLKPMVPKKPNTRVL* -1.673 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24108 RCACNRRRYHPRNPDDYLPM 20 SLAY-screened peptide P2458 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTGCGCCTGTAACCGCCGGCGCTATCATCCTAGGAATCCTGATGACTACCTGCCCATGTAA RCACNRRRYHPRNPDDYLPM* -1.673 0.000544 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24109 PLEVLPCFRWPNFLPYILAA 20 SLAY-screened peptide P2459 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCGAGGTGCTGCCCTGCTTTAGGTGGCCTAACTTTCTCCCTTACATTCTGGCTGCGTAA PLEVLPCFRWPNFLPYILAA* -1.673 0.025664 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24110 TAAFGPFLACPLGINSYTFT 20 SLAY-screened peptide P2460 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGCTGCTTTCGGGCCCTTTTTGGCCTGTCCGCTTGGCATTAACAGCTATACGTTCACGTAA TAAFGPFLACPLGINSYTFT* -1.673 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24111 VNRYARFYHSTVYIRIPCMI 20 SLAY-screened peptide P2461 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCAATCGGTACGCCCGTTTCTATCATTCCACTGTGTACATTCGCATTCCCTGTATGATCTAA VNRYARFYHSTVYIRIPCMI* -1.673 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24112 CVPDKSVLTLIGYTHD 16 SLAY-screened peptide P2462 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGTGCCGGATAAGTCCGTTCTCACGCTCATCGGTTATACGCATGACTAGCCCTCCACCTAA CVPDKSVLTLIGYTHD*PST* -1.673 0.003049 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24113 TPHYFRNQPLFHQTHITYLA 20 SLAY-screened peptide P2463 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCCGCATTATTTTCGTAATCAGCCCTTGTTCCATCAGACCCATATCACTTATCTTGCGTAA TPHYFRNQPLFHQTHITYLA* -1.673 0.008353 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24114 IVNGLFVQTPTTLPILHTAS 20 SLAY-screened peptide P2464 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGTTAACGGGCTGTTTGTTCAGACTCCGACCACTCTCCCCATTTTGCACACTGCTAGCTAA IVNGLFVQTPTTLPILHTAS* -1.673 0.000102 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24115 THLRAPPCMGDFIWFVLFSF 20 SLAY-screened peptide P2465 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCACCTCCGTGCGCCCCCCTGCATGGGGGATTTCATTTGGTTTGTTCTCTTTTCCTTCTAA THLRAPPCMGDFIWFVLFSF* -1.673 0.013461 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24116 TANQPIAFFPRLMLTSRLVPN 21 SLAY-screened peptide P2466 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGCCAACCAGCCTATCGCCTTCTTTCCCCGTCTTATGCTCACAAGTCGACTCGTTCCTAAC TANQPIAFFPRLMLTSRLVPN -1.672 0.001371 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24117 ASYRPSCSSVLYDLKPAQGG 20 SLAY-screened peptide P2467 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGTCTTATCGCCCGTCGTGTTCTAGCGTCTTGTACGACCTCAAGCCCGCCCAGGGTGGTTAA ASYRPSCSSVLYDLKPAQGG* -1.672 0.023835 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24118 THSPLVSFIMPPPLTPLCLV 20 SLAY-screened peptide P2468 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCATTCCCCGCTGGTCTCCTTCATCATGCCGCCGCCGCTTACTCCCCTCTGTTTGGTCTAA THSPLVSFIMPPPLTPLCLV* -1.672 0.000649 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24119 SMPPSRINYVGLHLDKRMLA 20 SLAY-screened peptide P2469 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCATGCCGCCTTCGCGTATCAACTACGTCGGCTTGCACCTTGACAAGCGGATGCTCGCGTAA SMPPSRINYVGLHLDKRMLA* -1.671 8e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24120 NKNISSLLQHR 11 SLAY-screened peptide P2470 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACAAGAATATTTCTTCCCTCTTGCAGCATCGGTAGGGTCCTTCCAGCGATGCTCGGCCTTAA NKNISSLLQHR*GPSSDARP* -1.671 0.014222 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24121 GLLSPWSVRFNYHDPDADHL 20 SLAY-screened peptide P2471 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCTGCTCTCTCCGTGGTCTGTGCGCTTCAATTACCATGACCCGGACGCTGATCACTTGTAA GLLSPWSVRFNYHDPDADHL* -1.671 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24122 SMNLNCPMVSCSHRDCLVVI 20 SLAY-screened peptide P2472 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTATGAACCTTAACTGCCCGATGGTTTCCTGTTCTCACCGTGACTGCCTCGTGGTTATTTAA SMNLNCPMVSCSHRDCLVVI* -1.67 0.000474 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24123 LIAARLFDCPLRQDSHCTWI 20 SLAY-screened peptide P2473 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTATCGCGGCGCGCCTCTTTGACTGTCCTCTTCGCCAGGATTCGCATTGTACCTGGATTTAA LIAARLFDCPLRQDSHCTWI* -1.67 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24124 VLVIASSYYF 10 SLAY-screened peptide P2474 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCTCGTTATTGCCAGCTCCTATTACTTCTAGCATCTGCATATCTATCTTGACCCGTCTTAA VLVIASSYYF*HLHIYLDPS* -1.67 1.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24125 RDRLQIIPTYGLSTVPLRGI 20 SLAY-screened peptide P2475 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGGACCGGCTCCAGATCATCCCTACTTACGGCCTCAGTACCGTTCCCCTCCGTGGCATTTAA RDRLQIIPTYGLSTVPLRGI* -1.669 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24126 IQMGIHRLTITNNYFTTFLY 20 SLAY-screened peptide P2476 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCAGATGGGTATTCACCGTTTGACGATTACCAATAACTATTTTACTACGTTTTTGTATTAA IQMGIHRLTITNNYFTTFLY* -1.668 0.002182 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24127 LENAAYTDYLDLPEHPCNST 20 SLAY-screened peptide P2477 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGAGAACGCCGCCTATACCGACTACCTTGATCTTCCCGAGCATCCCTGTAATAGCACCTAA LENAAYTDYLDLPEHPCNST* -1.668 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24128 YFRVPPTHLMLTPLVLYTLA 20 SLAY-screened peptide P2478 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTTCCGGGTCCCTCCCACCCACCTGATGCTGACTCCCTTGGTCCTCTACACTCTGGCGTAA YFRVPPTHLMLTPLVLYTLA* -1.668 0.021252 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24129 RSHSARLPALILPAPASLVIN 21 SLAY-screened peptide P2479 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTCGCACTCCGCGAGGCTCCCTGCTCTGATCTTACCCGCGCCTGCCTCCTTGGTGATTAAC RSHSARLPALILPAPASLVIN -1.668 0.004286 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24130 PTNSVHAYGIF 11 SLAY-screened peptide P2480 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACTAACTCGGTTCATGCGTATGGCATTTTCTAGGGCGTGATGCACCTGGTGACTGTCTAA PTNSVHAYGIF*GVMHLVTV* -1.668 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24131 RFTLNCFLGTRIRRKIGCSR 20 SLAY-screened peptide P2481 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTTCACTTTGAACTGTTTTCTCGGCACTCGCATCCGTCGCAAGATTGGGTGTAGCCGCTAA RFTLNCFLGTRIRRKIGCSR* -1.667 0.002099 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24132 LCGGSGPDCSDQSDNLANTI 20 SLAY-screened peptide P2482 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTTGCGGTGGCAGCGGTCCGGATTGCAGTGATCAGAGCGATAACCTGGCTAACACGATTTAA LCGGSGPDCSDQSDNLANTI* -1.667 0.000419 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24133 CWYIDRPALSRFRPVRTLLA 20 SLAY-screened peptide P2483 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTGGTATATCGATCGTCCGGCTCTCTCGCGCTTCCGGCCGGTTAGGACCCTGCTTGCTTAA CWYIDRPALSRFRPVRTLLA* -1.666 0.014851 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24134 SSPAAPRGRSPQSSRVG 17 SLAY-screened peptide P2484 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAGTCCGGCTGCGCCGCGCGGTCGCTCCCCTCAGAGTAGCCGCGTTGGTTAGAATGCGTAA SSPAAPRGRSPQSSRVG*NA* -1.666 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24135 CTTTALANFTLSRPKFDKIS 20 SLAY-screened peptide P2485 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCACCACTACCGCCCTCGCGAATTTTACTTTGAGTAGGCCTAAGTTTGATAAGATCTCTTAA CTTTALANFTLSRPKFDKIS* -1.666 0.002975 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24136 CFHYDYCCSCQSHPHWLLFA 20 SLAY-screened peptide P2486 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTTCCACTACGATTACTGCTGCTCTTGTCAGTCCCACCCCCACTGGCTCCTCTTTGCTTAA CFHYDYCCSCQSHPHWLLFA* -1.666 0.000157 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24137 LPKTSLRFRANAGPTPHPST 20 SLAY-screened peptide P2487 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCCAAGACCAGCCTCCGCTTTCGGGCCAATGCTGGTCCCACTCCCCACCCGTCGACCTAA LPKTSLRFRANAGPTPHPST* -1.666 0.017517 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24138 LSYVSPGAGYSPASDTPTRA 20 SLAY-screened peptide P2488 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTCCTACGTCTCTCCCGGGGCTGGGTACTCCCCGGCTAGCGACACGCCTACCCGGGCTTAA LSYVSPGAGYSPASDTPTRA* -1.666 0.003337 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24139 SLAIILLSYPDFSMHSILAF 20 SLAY-screened peptide P2489 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTTGGCCATCATCCTGTTGTCCTATCCGGACTTCTCCATGCATTCCATTCTTGCCTTTTAA SLAIILLSYPDFSMHSILAF* -1.665 1.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24140 PSSRR 5 SLAY-screened peptide P2490 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGCTCGCGGCGGTAGACTATGTAGATCAACCACACCTTGAACTGTAATCGCACTCATTAA PSSRR*TM*INHTLNCNRTH* -1.665 0.046519 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24141 AAVTAIRQLFNCLIMTARCS 20 SLAY-screened peptide P2491 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGCCGTCACCGCCATCAGGCAGCTTTTCAATTGTCTTATTATGACCGCCCGCTGTTCCTAA AAVTAIRQLFNCLIMTARCS* -1.665 0.004576 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24142 SQTRPKHSLVMPFSYTLTMP 20 SLAY-screened peptide P2492 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCAGACTCGTCCGAAGCATAGTCTCGTTATGCCGTTTAGTTATACGTTGACCATGCCGTAA SQTRPKHSLVMPFSYTLTMP* -1.665 0.000224 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24143 CIKCYSVYCLLFNYRHTGVT 20 SLAY-screened peptide P2493 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTATCAAGTGTTATTCTGTGTATTGCCTGCTGTTCAATTACCGTCATACCGGTGTCACGTAA CIKCYSVYCLLFNYRHTGVT* -1.665 0.014298 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24144 LANPCNIYNHAPMYQSLRYA 20 SLAY-screened peptide P2494 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGCCAATCCTTGCAACATCTACAATCATGCGCCCATGTATCAGAGTCTTCGTTACGCCTAA LANPCNIYNHAPMYQSLRYA* -1.665 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24145 VVSVRVVREYTLADGFIMSC 20 SLAY-screened peptide P2495 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGGTTAGCGTCCGTGTGGTCCGTGAGTATACCCTTGCTGATGGTTTTATCATGTCCTGTTAA VVSVRVVREYTLADGFIMSC* -1.665 0.000188 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24146 HRHLCSQERHTRSARVDRMS 20 SLAY-screened peptide P2496 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCGGCACCTGTGCTCCCAGGAGCGTCATACTCGCTCCGCGCGCGTCGACCGCATGTCCTAA HRHLCSQERHTRSARVDRMS* -1.665 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24147 MVHSSDSTWFSFPGELHDSP 20 SLAY-screened peptide P2497 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGGTGCACTCTTCTGACTCCACTTGGTTTTCGTTCCCCGGTGAGTTGCATGATAGCCCGTAA MVHSSDSTWFSFPGELHDSP* -1.664 7.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24148 SPAPLLCRVRPRSRRTTASSN 21 SLAY-screened peptide P2498 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCCTGCCCCCTTACTGTGCAGGGTCCGACCTAGAAGCAGACGGACAACAGCGTCTTCTAAC SPAPLLCRVRPRSRRTTASSN -1.664 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24149 PYHRPFSPPNYDQSNGHSGT 20 SLAY-screened peptide P2499 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTACCACCGTCCGTTTTCTCCCCCTAATTATGATCAGAGCAATGGTCACTCTGGGACCTAA PYHRPFSPPNYDQSNGHSGT* -1.664 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24150 PLRRIASKLLHSFICPCW 18 SLAY-screened peptide P2500 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCCGGCGCATCGCTTCTAAGCTCCTGCACTCTTTTATCTGCCCCTGTTGGTAGCCCTAA PLRRIASKLLHSFICPCW*P* -1.664 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24151 PRYPYRVSTLLPSDSRLGRS 20 SLAY-screened peptide P2501 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCGCTACCCTTATCGCGTGTCGACCCTGCTCCCGTCTGACTCGCGGCTTGGGAGGTCGTAA PRYPYRVSTLLPSDSRLGRS* -1.664 7.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24152 LPGLTTHCVYDGPTSDTSRA 20 SLAY-screened peptide P2502 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCGGGCCTCACTACTCATTGTGTCTATGACGGTCCCACGAGTGACACCTCTAGGGCTTAA LPGLTTHCVYDGPTSDTSRA* -1.664 0.000149 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24153 HCGMPDWSDYSFRYI 15 SLAY-screened peptide P2503 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGTGGCATGCCCGATTGGTCGGATTACTCTTTTCGCTATATCTAGTTCCAGACTCCGTAA HCGMPDWSDYSFRYI*FQTP* -1.664 0.024309 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24154 HRHMQAVPTPQVPARRTRGF 20 SLAY-screened peptide P2504 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGCCACATGCAGGCTGTTCCCACGCCCCAGGTCCCTGCTCGTCGGACTCGCGGCTTCTAA HRHMQAVPTPQVPARRTRGF* -1.663 0.023872 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24155 PQA 3 SLAY-screened peptide P2505 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCAAGCATAAGACCAACTGTCGTAAGTACGGGTCGAAGAATATGCATAATCTCAACTAACT PQA*DQLS*VRVEEYA*SQLT -1.663 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24156 PDSGLVPFTHFGTAFYIHHA 20 SLAY-screened peptide P2506 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGATTCGGGCCTTGTCCCTTTCACCCATTTTGGCACGGCTTTTTATATCCACCACGCCTAA PDSGLVPFTHFGTAFYIHHA* -1.663 2.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24157 TNALY 5 SLAY-screened peptide P2507 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGAATGCCCTGTACTAGCGGCGCTTTCCCCTGTGGCTTTGGCCCGGTTGCGCCATCCACTAA TNALY*RRFPLWLWPGCAIH* -1.663 3.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24158 PTPIANRTDTPYLFTVYYYT 20 SLAY-screened peptide P2508 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACCCCTATTGCTAATAGGACGGATACCCCCTATTTGTTTACCGTTTATTATTACACGTAA PTPIANRTDTPYLFTVYYYT* -1.663 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24159 APAIFADYHPVCLFYARNTM 20 SLAY-screened peptide P2509 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCCCGCTATCTTTGCTGATTATCACCCCGTTTGCCTGTTCTATGCTCGGAACACTATGTAA APAIFADYHPVCLFYARNTM* -1.662 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24160 STPAHPRASDFYYTASFYRN 20 SLAY-screened peptide P2510 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTACCCCCGCTCACCCTAGGGCGTCGGATTTTTACTACACTGCTTCGTTCTACCGTAACTAA STPAHPRASDFYYTASFYRN* -1.662 0.00536 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24161 NIDTWPSHFRLHTRFGRATP 20 SLAY-screened peptide P2511 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACATCGATACGTGGCCCTCCCATTTCCGCTTGCATACTCGCTTTGGCCGCGCTACTCCCTAA NIDTWPSHFRLHTRFGRATP* -1.662 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24162 AKLILNVGILLRRRTSSLSFN 21 SLAY-screened peptide P2512 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCAAGCTCATTTTGAACGTGGGCATATTACTGAGACGCCGAACTTCCTCGTTAAGCTTTAAC AKLILNVGILLRRRTSSLSFN -1.662 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24163 CWHPKTKKGRYLVNHMPVVF 20 SLAY-screened peptide P2513 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTGGCACCCCAAGACTAAGAAGGGGCGCTACCTTGTCAACCACATGCCCGTTGTTTTCTAA CWHPKTKKGRYLVNHMPVVF* -1.661 0.000397 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24164 RPGSACPSSG 10 SLAY-screened peptide P2514 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCCCGGCTCGGCCTGCCCGTCGAGCGGTTAGATCATCTTGCCTTTTTCCCAGTCTAGCTAA RPGSACPSSG*IILPFSQSS* -1.661 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24165 LFTAYSPRVITMVHTSRVIT 20 SLAY-screened peptide P2515 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTTCACCGCGTACAGCCCCAGGGTTATTACTATGGTCCACACGAGCCGCGTGATCACGTAA LFTAYSPRVITMVHTSRVIT* -1.661 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24166 CHRLSRNDASHCFPSARVPGN 21 SLAY-screened peptide P2516 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCACCGTCTGTCTCGCAATGACGCCTCGCACTGTTTTCCTTCAGCACGTGTCCCCGGTAAC CHRLSRNDASHCFPSARVPGN -1.66 0.001742 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24167 RCFTDDTDLLSPSMIVYYSI 20 SLAY-screened peptide P2517 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTGCTTCACTGACGATACTGATCTTCTCAGCCCTAGTATGATTGTCTATTACAGTATTTAA RCFTDDTDLLSPSMIVYYSI* -1.66 0.000653 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24168 GFLSTPPLRERRLFCIPRPS 20 SLAY-screened peptide P2518 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTTCCTTTCCACCCCGCCCCTTCGCGAGCGTAGGCTGTTTTGTATCCCGCGTCCCTCGTAA GFLSTPPLRERRLFCIPRPS* -1.659 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24169 SIDHSYLFWYI 11 SLAY-screened peptide P2519 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGATTGACCATAGTTACCTCTTCTGGTATATCTAGCCTACTGCTCATGTTAACATGAGGTAA SIDHSYLFWYI*PTAHVNMR* -1.659 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24170 IVAYYVYTYFPGRSLNNIPP 20 SLAY-screened peptide P2520 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGTGGCTTACTATGTTTACACCTACTTTCCGGGGCGTTCGCTGAATAACATCCCGCCTTAA IVAYYVYTYFPGRSLNNIPP* -1.658 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24171 SRPPSNTIAITFHNNRTFFF 20 SLAY-screened peptide P2521 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCAGGCCCCCTTCCAACACGATCGCGATTACGTTCCATAACAACCGTACTTTCTTTTTTTAA SRPPSNTIAITFHNNRTFFF* -1.658 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24172 DFLPCHLFHRPHNSNGEYVG 20 SLAY-screened peptide P2522 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTTCTTGCCCTGCCATCTTTTCCACCGTCCCCATAATTCTAACGGTGAGTATGTTGGCTAA DFLPCHLFHRPHNSNGEYVG* -1.658 0.001482 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24173 LSQSASPCYC 10 SLAY-screened peptide P2523 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCCCAGAGTGCTTCTCCTTGCTATTGTTAGAGGTACGCGCTCGATGATGGTGACTCCTAA LSQSASPCYC*RYALDDGDS* -1.658 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24174 RMSQLRALL 9 SLAY-screened peptide P2524 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCATGAGCCAGCTCCGTGCCCTTCTCTAGTCTTGTTCCACTAAGGCGGAGCGTTGCGTCTAA RMSQLRALL*SCSTKAERCV* -1.658 0.000249 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24175 YARMNITLVGMCQVFYFIIR 20 SLAY-screened peptide P2525 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATGCGAGGATGAACATTACCCTCGTTGGTATGTGTCAGGTCTTTTATTTCATTATCCGCTAA YARMNITLVGMCQVFYFIIR* -1.657 0.001025 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24176 IPYQHLFDVINRFLHIRKMN 20 SLAY-screened peptide P2526 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCCCTATCAGCATTTGTTTGACGTCATTAACCGTTTCCTTCACATTCGCAAGATGAACTAA IPYQHLFDVINRFLHIRKMN* -1.657 0.0005 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24177 PTDKTSARSNDSCLHVANRV 20 SLAY-screened peptide P2527 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACCGATAAGACCAGCGCTCGTAGCAACGATAGCTGTTTGCATGTTGCCAACAGGGTGTAA PTDKTSARSNDSCLHVANRV* -1.657 0.006651 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24178 TLFDWYMGCYPLAQRDIIYL 20 SLAY-screened peptide P2528 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTGTTCGACTGGTACATGGGTTGTTATCCCCTGGCTCAGCGCGATATTATCTACTTGTAA TLFDWYMGCYPLAQRDIIYL* -1.656 0.015514 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24179 QSYSNNFHLIHRTDLPFTCT 20 SLAY-screened peptide P2529 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGAGTTACTCCAACAACTTTCATCTTATCCATCGGACCGATCTTCCTTTTACTTGCACGTAA QSYSNNFHLIHRTDLPFTCT* -1.656 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24180 PLMYCHPYGYPYRAFIFDTL 20 SLAY-screened peptide P2530 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTGATGTACTGCCACCCGTATGGGTATCCGTATCGCGCCTTCATCTTCGACACTTTGTAA PLMYCHPYGYPYRAFIFDTL* -1.656 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24181 HWIGYALTLEALHCLFYSHP 20 SLAY-screened peptide P2531 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGGATCGGGTACGCCCTTACGCTTGAGGCTCTTCACTGCCTCTTTTATTCTCATCCTTAA HWIGYALTLEALHCLFYSHP* -1.656 0.044457 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24182 PLPCWLV 7 SLAY-screened peptide P2532 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTGCCCTGCTGGCTTGTCTAGGCGGCCTCGCTGTCCAGGATGTGTGCTTTCCGCAGGTAA PLPCWLV*AASLSRMCAFRR* -1.655 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24183 GCSPTPLGIFFPSLVVRQIR 20 SLAY-screened peptide P2533 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGTGCAGCCCTACGCCTCTTGGTATTTTCTTCCCCAGTCTCGTTGTCCGCCAGATCCGCTAA GCSPTPLGIFFPSLVVRQIR* -1.655 0.012436 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24184 RFFLFFIRRRTTFKAWSINS 20 SLAY-screened peptide P2534 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTTCTTCTTGTTCTTTATTCGCCGGAGGACGACCTTTAAGGCGTGGAGCATCAACAGCTAA RFFLFFIRRRTTFKAWSINS* -1.655 0.000421 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24185 RLAGHATFHVTLLVYLSTRN 20 SLAY-screened peptide P2535 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCTTGCTGGCCATGCCACCTTCCACGTGACTCTCCTGGTCTATCTCTCCACTAGGAACTAA RLAGHATFHVTLLVYLSTRN* -1.655 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24186 SGACWDKTDTYQLG 14 SLAY-screened peptide P2536 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGGCGCCTGCTGGGATAAGACGGACACCTATCAGCTTGGTTAGTTCTCTATCGGGTCTTAA SGACWDKTDTYQLG*FSIGS* -1.654 0.000967 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24187 SAIANASVVSSIACAIGHIT 20 SLAY-screened peptide P2537 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGCTATCGCTAATGCTTCCGTGGTGTCGTCCATTGCCTGCGCTATTGGGCACATTACGTAA SAIANASVVSSIACAIGHIT* -1.654 0.031658 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24188 FLRPLVRSLLRRLLVMGWRRN 21 SLAY-screened peptide P2538 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTTGCGTCCGTTGGTTCGCTCCCTCCTCCGGAGGTTATTAGTTATGGGATGGCGCCGTAAC FLRPLVRSLLRRLLVMGWRRN -1.654 0.008316 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24189 PSVSNFPHRCD 11 SLAY-screened peptide P2539 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCTGTGTCTAACTTCCCGCACCGCTGCGATTAGCGTGCCTTGCGTTGTAGGACGGACTAA PSVSNFPHRCD*RALRCRTD* -1.654 0.000943 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24190 PRNDGSGYITPKIRTLFENQ 20 SLAY-screened peptide P2540 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCGGAATGATGGCTCCGGTTATATCACTCCGAAGATTCGGACCCTTTTTGAGAACCAGTAA PRNDGSGYITPKIRTLFENQ* -1.654 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24191 PSRHVDRPGNNLGNVNSATD 20 SLAY-screened peptide P2541 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAGCAGGCATGTGGACAGGCCGGGCAACAATTTGGGCAATGTTAATAGCGCTACGGACTAA PSRHVDRPGNNLGNVNSATD* -1.653 0.026102 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24192 FLVESDTAAAYNPETCDPNV 20 SLAY-screened peptide P2542 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCTGGTGGAGTCTGATACCGCCGCCGCCTACAACCCCGAGACTTGCGACCCGAATGTTTAA FLVESDTAAAYNPETCDPNV* -1.653 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24193 PRTMSYNSACTCSELNCLDP 20 SLAY-screened peptide P2543 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGGACCATGAGTTATAACTCCGCCTGCACGTGTTCCGAGCTGAACTGTCTGGATCCCTAA PRTMSYNSACTCSELNCLDP* -1.653 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24194 NPHGPIRTNNDRHVTREPTY 20 SLAY-screened peptide P2544 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACCCCCACGGCCCTATTCGCACGAACAACGATCGTCATGTCACCCGGGAGCCTACCTACTAA NPHGPIRTNNDRHVTREPTY* -1.653 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24195 SPQGL 5 SLAY-screened peptide P2545 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCCCCAGGGCCTTTAGCACCACCATTAGCGTATGGACAACCAGCTCGCCTTTAGTCAGTAA SPQGL*HHH*RMDNQLAFSQ* -1.652 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24196 RLSFNPPYPGLRAHLPGTFL 20 SLAY-screened peptide P2546 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCTGTCGTTTAACCCGCCCTATCCGGGCCTCCGCGCGCACCTCCCGGGCACTTTCCTCTAA RLSFNPPYPGLRAHLPGTFL* -1.652 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24197 TFNSVPAHGQVRNFYHHFIR 20 SLAY-screened peptide P2547 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTTTAACAGTGTGCCGGCTCATGGGCAGGTCCGTAACTTCTACCATCATTTTATCCGTTAA TFNSVPAHGQVRNFYHHFIR* -1.651 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24198 HCSPPVTWLWGSSIPHAYLL 20 SLAY-screened peptide P2548 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTGTTCCCCTCCCGTGACGTGGCTCTGGGGTAGCTCTATCCCTCACGCTTACCTCTTGTAA HCSPPVTWLWGSSIPHAYLL* -1.651 0.000104 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24199 CPIMFLQRSPVTMSLNPDSL 20 SLAY-screened peptide P2549 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCCATCATGTTCCTCCAGCGTAGCCCCGTTACCATGAGTCTTAACCCTGATTCCTTGTAA CPIMFLQRSPVTMSLNPDSL* -1.65 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24200 TTSLDTGMLHPRNTQTSNSD 20 SLAY-screened peptide P2550 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTACTTCCCTTGACACTGGCATGCTGCATCCTCGTAATACTCAGACGTCGAATTCTGATTAA TTSLDTGMLHPRNTQTSNSD* -1.65 0.0311 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24201 PPSTDLTQCHQPLPWRYLRM 20 SLAY-screened peptide P2551 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCCTAGTACGGACCTGACCCAGTGCCATCAGCCCCTCCCCTGGCGTTACCTGCGCATGTAA PPSTDLTQCHQPLPWRYLRM* -1.65 0.000707 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24202 LGGSRDMPFIHPSAADTCGH 20 SLAY-screened peptide P2552 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGGCGGTTCTCGCGACATGCCGTTTATTCATCCCTCGGCGGCGGATACTTGCGGTCATTAA LGGSRDMPFIHPSAADTCGH* -1.65 0.025147 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24203 GRSTGHLPWTIWKLFLCVAH 20 SLAY-screened peptide P2553 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCCGTTCTACTGGGCATCTTCCTTGGACTATCTGGAAGCTGTTTCTGTGTGTGGCCCACTAA GRSTGHLPWTIWKLFLCVAH* -1.65 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24204 THPPAH 6 SLAY-screened peptide P2554 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCACCCCCCCGCCCACTAGCCGCTTTCCAGTCAGGTTTTTTACCACACGGACTACTAGTAA THPPAH*PLSSQVFYHTDY** -1.65 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24205 LLPSPCRSV 9 SLAY-screened peptide P2555 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTTGCCCTCGCCTTGCAGGAGCGTCTAGTAGTTGCCCTTGGCCTTCAAGAATGTGCTCTAA LLPSPCRSV**LPLAFKNVL* -1.649 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24206 YYNRFGGRCSSL 12 SLAY-screened peptide P2556 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTACAACCGTTTCGGAGGTCGATGTTCGTCGTTATAAGATGTTGAATAATTCGAATAACTA YYNRFGGRCSSL*DVE*FE*L -1.648 0.003453 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24207 PRASNYALALDDFTPLPLTA 20 SLAY-screened peptide P2557 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAGGGCTAGTAATTACGCGCTTGCTCTGGATGACTTTACGCCTCTTCCGTTGACCGCTTAA PRASNYALALDDFTPLPLTA* -1.648 0.000359 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24208 RCHRYAANGQSHGLNESIHP 20 SLAY-screened peptide P2558 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTGCCACCGCTACGCGGCTAATGGGCAGTCTCATGGGTTGAATGAGTCTATCCACCCGTAA RCHRYAANGQSHGLNESIHP* -1.648 0.000146 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24209 HTRVNSHYSSCPSLGPRLRSN 21 SLAY-screened peptide P2559 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACGCGTGTCAATAGTCACTACAGTTCCTGCCCAAGTCTTGGGCCCCGTCTTCGATCTAAC HTRVNSHYSSCPSLGPRLRSN -1.648 0.001817 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24210 HTIDALPYDLFRSSPTCFQY 20 SLAY-screened peptide P2560 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACACCATTGATGCGCTGCCGTACGACCTGTTTCGCTCGTCGCCCACGTGCTTTCAGTACTAA HTIDALPYDLFRSSPTCFQY* -1.648 0.006516 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24211 RPYHCISILSMILVPRPMVT 20 SLAY-screened peptide P2561 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCCTACCATTGTATTTCTATTCTCTCCATGATCTTGGTTCCGCGGCCTATGGTGACTTAA RPYHCISILSMILVPRPMVT* -1.648 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24212 RYPFDTTHILPNPSADSYKC 20 SLAY-screened peptide P2562 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTATCCGTTCGATACCACCCACATCTTGCCCAACCCGTCTGCTGATTCTTATAAGTGCTAA RYPFDTTHILPNPSADSYKC* -1.647 0.002015 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24213 CHFHPHHYRVITSVPL 16 SLAY-screened peptide P2563 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCACTTCCATCCGCATCACTACCGGGTGATCACGTCGGTGCCTCTCTAGTTCGGTGATTAA CHFHPHHYRVITSVPL*FGD* -1.647 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24214 NLYHCPAHPLYITAAGNAIS 20 SLAY-screened peptide P2564 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTTGTACCATTGCCCGGCTCATCCTCTGTATATTACGGCTGCGGGTAATGCTATTTCCTAA NLYHCPAHPLYITAAGNAIS* -1.647 0.02038 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24215 TNVRYACYRLFGGIRICYCP 20 SLAY-screened peptide P2565 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTAACGTGCGCTATGCCTGTTATCGGTTGTTCGGGGGTATCCGCATCTGTTATTGTCCCTAA TNVRYACYRLFGGIRICYCP* -1.647 0.008385 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24216 HPDHGLSALFHTGMYAASL 19 SLAY-screened peptide P2566 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCCGGACCACGGTCTCTCTGCCCTTTTCCACACCGGTATGTATGCTGCTTCCTTGTAGTAA HPDHGLSALFHTGMYAASL** -1.647 0.026016 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24217 NETWPSPHGCRTVVAPMRIA 20 SLAY-screened peptide P2567 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGAGACGTGGCCCTCTCCCCATGGTTGCCGGACCGTGGTCGCCCCGATGCGGATCGCTTAA NETWPSPHGCRTVVAPMRIA* -1.647 0.001213 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24218 PTVRNANSFCLRKIILSRDW 20 SLAY-screened peptide P2568 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCACTGTTCGTAATGCCAATAGTTTTTGTTTGCGCAAGATCATTCTCAGTCGTGATTGGTAA PTVRNANSFCLRKIILSRDW* -1.646 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24219 RSMRRRYVHLTRRALHNNAC 20 SLAY-screened peptide P2569 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTCTATGCGCCGCCGCTACGTCCATCTTACTCGTCGGGCGCTTCATAACAATGCTTGCTAA RSMRRRYVHLTRRALHNNAC* -1.646 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24220 VSAGITRLLHLCDYFTTMCF 20 SLAY-screened peptide P2570 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGAGCGCTGGGATCACCAGGCTGCTCCATCTTTGTGACTACTTCACGACTATGTGCTTCTAA VSAGITRLLHLCDYFTTMCF* -1.645 0.01066 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24221 RAAEYLFALSLDPLLFYSYQ 20 SLAY-screened peptide P2571 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGGCTGCCGAGTATCTCTTTGCCCTGTCCCTCGATCCTCTTCTCTTCTACAGTTATCAGTAA RAAEYLFALSLDPLLFYSYQ* -1.645 0.011149 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24222 LPVYCWYTLITPICSCDEYV 20 SLAY-screened peptide P2572 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCCCGTCTACTGTTGGTACACCCTCATTACGCCTATCTGTAGCTGCGATGAGTACGTTTAA LPVYCWYTLITPICSCDEYV* -1.644 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24223 PHRACPSGSSPFPLLPFFFLN 21 SLAY-screened peptide P2573 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCATCGGGCCTGCCCCTCGGGATCGTCGCCGTTCCCACTGCTACCCTTTTTCTTTCTTAAC PHRACPSGSSPFPLLPFFFLN -1.644 0.005076 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24224 HCTSYQHNTPSMISNYYDIM 20 SLAY-screened peptide P2574 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGCACCAGTTATCAGCACAATACGCCCTCCATGATCTCGAATTACTATGATATCATGTAA HCTSYQHNTPSMISNYYDIM* -1.644 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24225 NYDALRLFDLRNYQFTNNAS 20 SLAY-screened peptide P2575 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTATGATGCTCTCCGCCTGTTCGATCTTCGCAACTATCAGTTCACTAATAATGCCTCGTAA NYDALRLFDLRNYQFTNNAS* -1.643 0.005656 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24226 SNSRENHPTVHLCSVSNVLY 20 SLAY-screened peptide P2576 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTAACTCTAGGGAGAATCACCCGACGGTGCATTTGTGCTCTGTTAGCAACGTGCTGTATTAA SNSRENHPTVHLCSVSNVLY* -1.643 0.004283 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24227 INLMVMYTTASSLSHYWDLD 20 SLAY-screened peptide P2577 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTAATCTCATGGTCATGTATACTACGGCGTCGAGCCTGTCTCACTACTGGGACCTGGATTAA INLMVMYTTASSLSHYWDLD* -1.643 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24228 LQVVLPYGCYSSNYFCYNLL 20 SLAY-screened peptide P2578 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCAGGTTGTTCTGCCTTATGGTTGTTACTCGTCCAACTATTTTTGTTACAATCTCCTTTAA LQVVLPYGCYSSNYFCYNLL* -1.643 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24229 CECANICYYPPYWNII 16 SLAY-screened peptide P2579 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGAGTGCGCTAATATTTGTTATTACCCTCCCTATTGGAACATCATTTAGTATGTCTAGTAA CECANICYYPPYWNII*YV** -1.643 0.015522 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24230 RATSPVIS 8 SLAY-screened peptide P2580 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGCTACTTCTCCTGTTATCTCTTAGTGTCGTGACCACTCTGTGGCGGTGTGCATGAACTAA RATSPVIS*CRDHSVAVCMN* -1.643 0.000374 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24231 TRIILDGYPSNPLNALANDN 20 SLAY-screened peptide P2581 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCGCATCATCTTGGATGGCTATCCGTCTAATCCTCTGAATGCCTTGGCTAACGACAATTAA TRIILDGYPSNPLNALANDN* -1.643 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24232 LSGCYAPSTHVLHAWEKISV 20 SLAY-screened peptide P2582 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAGCGGGTGTTATGCTCCTAGTACTCACGTGCTCCATGCCTGGGAGAAGATCAGCGTCTAA LSGCYAPSTHVLHAWEKISV* -1.642 0.000666 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24233 ALHFSLLLFRFCRSRTCRHC 20 SLAY-screened peptide P2583 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTTTGCATTTCAGTCTTCTCCTCTTTAGGTTCTGTCGCTCGCGTACGTGTAGGCATTGTTAA ALHFSLLLFRFCRSRTCRHC* -1.642 0.000152 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24234 PQAMGRRITSRSGTHTWKMC 20 SLAY-screened peptide P2584 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCAGGCCATGGGCCGGCGTATTACCTCCCGTTCGGGTACCCATACCTGGAAGATGTGTTAA PQAMGRRITSRSGTHTWKMC* -1.642 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24235 PLSCTLEVVSNYCTGHFKCP 20 SLAY-screened peptide P2585 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCAGCTGCACCCTCGAGGTGGTGTCTAACTACTGTACTGGGCACTTCAAGTGTCCTTAA PLSCTLEVVSNYCTGHFKCP* -1.642 0.000156 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24236 HAPSSTLTLGNLPQSDHTVA 20 SLAY-screened peptide P2586 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATGCGCCTTCGTCTACGCTTACCTTGGGTAATTTGCCCCAGTCCGACCACACGGTCGCTTAA HAPSSTLTLGNLPQSDHTVA* -1.642 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24237 RLAGTLGHTLTVFYARSIAR 20 SLAY-screened peptide P2587 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTTGGCTGGCACCCTGGGCCACACCCTGACGGTTTTCTATGCGCGGTCTATCGCCCGGTAA RLAGTLGHTLTVFYARSIAR* -1.641 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24238 PAWSYSLFWLYFTVYMCFEK 20 SLAY-screened peptide P2588 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCGTGGTCGTACTCCCTGTTTTGGCTTTATTTCACCGTTTATATGTGCTTTGAGAAGTAA PAWSYSLFWLYFTVYMCFEK* -1.641 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24239 HHHHHFNIPLNTVPYSYCLA 20 SLAY-screened peptide P2589 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCATCATCACCACTTCAACATCCCTCTTAATACCGTGCCTTATTCGTATTGCCTCGCTTAA HHHHHFNIPLNTVPYSYCLA* -1.641 0.01151 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24240 CDLYFYL 7 SLAY-screened peptide P2590 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGATCTTTATTTTTATTTGTAGCTTGCGGGGCATAATATTCGTTTCAATCATATTCTGTAA CDLYFYL*LAGHNIRFNHIL* -1.641 0.000741 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24241 STHGDAIYVHSCLWCVIHTR 20 SLAY-screened peptide P2591 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCACGCATGGCGACGCGATTTACGTTCATTCTTGCCTGTGGTGTGTCATTCATACGCGTTAA STHGDAIYVHSCLWCVIHTR* -1.641 5.5e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24242 CLRSSHL 7 SLAY-screened peptide P2592 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCTCAGGTCTTCTCACCTCTAGCGTGACTGTGCTGCGCATTGTACTGTCGTTATCCCTTAA CLRSSHL*RDCAAHCTVVIP* -1.641 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24243 KYRMTPRFPTICHRIHTMYN 20 SLAY-screened peptide P2593 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGTACAGGATGACGCCCCGTTTTCCCACTATCTGTCATCGGATTCATACGATGTATAATTAA KYRMTPRFPTICHRIHTMYN* -1.639 0.000181 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24244 DPTAAMYTPCTATVVT 16 SLAY-screened peptide P2594 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATCCTACCGCCGCCATGTACACCCCGTGCACCGCCACCGTCGTCACGTAGAATCGCGCCTAA DPTAAMYTPCTATVVT*NRA* -1.639 0.017461 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24245 HPVHTTFSNPCDITHTPYTFK 21 SLAY-screened peptide P2595 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCCCGTCCATACCACCTTTTCCAACCCCTGCGACATCACTCACACGCCGTACACTTTCAAG HPVHTTFSNPCDITHTPYTFK -1.639 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24246 TGRRSNVSKHSANIYVNSKG 20 SLAY-screened peptide P2596 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGGCCGCAGGTCCAATGTTAGTAAGCATTCGGCTAACATTTATGTTAACTCTAAGGGCTAA TGRRSNVSKHSANIYVNSKG* -1.638 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24247 PLSSNGDCHLFIHYDYV 17 SLAY-screened peptide P2597 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTTGTCTTCTAATGGTGACTGCCATCTGTTTATTCACTACGACTATGTTTAGCGCCATTAA PLSSNGDCHLFIHYDYV*RH* -1.638 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24248 RQRFGAIYTATSKYDCTCIR 20 SLAY-screened peptide P2598 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCAGAGGTTTGGCGCTATCTATACGGCTACGTCTAAGTATGACTGCACCTGTATCAGGTAA RQRFGAIYTATSKYDCTCIR* -1.638 0.03453 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24249 QQLKHYLNLMSLYLHAFLRH 20 SLAY-screened peptide P2599 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCAGCTTAAGCACTACCTTAACCTTATGTCCCTGTACCTGCATGCGTTTCTCCGCCACTAA QQLKHYLNLMSLYLHAFLRH* -1.638 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24250 LHHSYTPVFPFNNSLPCVLF 20 SLAY-screened peptide P2600 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCCATCATTCTTATACTCCGGTGTTCCCGTTCAATAACAGTCTTCCCTGCGTCTTGTTCTAA LHHSYTPVFPFNNSLPCVLF* -1.637 0.000723 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24251 CETPVLQKPFLSDAHTRGPR 20 SLAY-screened peptide P2601 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGAGACTCCTGTCCTTCAGAAGCCTTTTCTCTCCGATGCGCATACGCGCGGTCCTCGGTAA CETPVLQKPFLSDAHTRGPR* -1.637 0.00272 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24252 LADKLRGLLCKYCYRTQICT 20 SLAY-screened peptide P2602 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGCTGACAAGCTTCGGGGGCTGCTCTGCAAGTATTGCTACCGCACTCAGATTTGCACGTAA LADKLRGLLCKYCYRTQICT* -1.636 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24253 YRNRARRTFDLHLPPLLVTT 20 SLAY-screened peptide P2603 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATAGGAACCGCGCTCGTCGTACCTTTGACCTTCACCTGCCTCCTTTGCTGGTCACTACTTAA YRNRARRTFDLHLPPLLVTT* -1.636 0.002006 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24254 RDPT 4 SLAY-screened peptide P2604 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGACCCGACTTAGACTGCTTTGACCCATAATCGTATTGTCCTGGGCTCTGGGATGTGCTAA RDPT*TALTHNRIVLGSGMC* -1.636 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24255 NDAGIIERPNVMHRITRDPF 20 SLAY-screened peptide P2605 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGATGCTGGGATTATTGAGCGCCCCAATGTCATGCACCGCATTACCCGTGACCCCTTCTAA NDAGIIERPNVMHRITRDPF* -1.636 1.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24256 RPFPKHDFNAILRL 14 SLAY-screened peptide P2606 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCCTTTTCCTAAGCACGACTTCAACGCCATTTTGCGTCTTTAGAAGCTTGCCGAGGATTAA RPFPKHDFNAILRL*KLAED* -1.636 7.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24257 NTIRSHLSLLYLIPIEHHEA 20 SLAY-screened peptide P2607 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATACCATCCGCAGTCACCTTTCCCTTCTTTACCTTATTCCTATTGAGCATCATGAGGCCTAA NTIRSHLSLLYLIPIEHHEA* -1.635 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24258 SYLSQYKSNYIRLLGISTSP 20 SLAY-screened peptide P2608 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTATCTCTCCCAGTATAAGTCCAATTATATTAGGCTGCTGGGCATCTCGACGAGTCCCTAA SYLSQYKSNYIRLLGISTSP* -1.635 0.000385 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24259 PSADFFNLKNKCYNYIELFV 20 SLAY-screened peptide P2609 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCGGCCGACTTTTTCAATCTTAAGAATAAGTGCTATAACTATATTGAGTTGTTTGTTTAA PSADFFNLKNKCYNYIELFV* -1.634 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24260 TQYECFTAIHNRASFDHLLV 20 SLAY-screened peptide P2610 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCAGTATGAGTGTTTTACTGCGATCCACAATCGCGCCTCCTTTGACCATCTTTTGGTTTAA TQYECFTAIHNRASFDHLLV* -1.634 0.002696 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24261 YRFLPLEVVLLHGW 14 SLAY-screened peptide P2611 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCGCTTTTTACCGCTGGAAGTCGTTTTACTTCATGGTTGGTAACACGAACAGGTAGTAACT YRFLPLEVVLLHGW*HEQVVT -1.634 0.021318 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24262 FATGWAGFRDCLTMNSTHIN 20 SLAY-screened peptide P2612 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTGCTACCGGGTGGGCTGGTTTCCGTGATTGCTTGACCATGAATAGTACGCACATTAACTAA FATGWAGFRDCLTMNSTHIN* -1.634 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24263 VPHTPNVNYSGVQSVTEINV 20 SLAY-screened peptide P2613 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCCTCATACTCCTAACGTTAACTACTCGGGGGTGCAGTCCGTTACCGAGATTAACGTGTAA VPHTPNVNYSGVQSVTEINV* -1.634 0.017019 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24264 YSLMLSCYSTRYIFIGENSP 20 SLAY-screened peptide P2614 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATTCGTTGATGCTGTCCTGCTACTCCACTCGTTATATTTTCATCGGGGAGAACAGCCCCTAA YSLMLSCYSTRYIFIGENSP* -1.634 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24265 CSHTWFVDITIFHCSDFSG 19 SLAY-screened peptide P2615 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTCGCACACCTGGTTCGTGGATATTACTATTTTCCACTGCAGTGATTTCAGTGGTTAGTAA CSHTWFVDITIFHCSDFSG** -1.634 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24266 ISGTLPHGNTWRKCAHIPDY 20 SLAY-screened peptide P2616 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAGCGGTACCCTTCCCCATGGCAACACTTGGAGGAAGTGCGCCCACATTCCGGATTACTAA ISGTLPHGNTWRKCAHIPDY* -1.633 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24267 SPRVHSLEEGKYQGAMIDSD 20 SLAY-screened peptide P2617 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCCCCGGGTTCACTCCTTGGAGGAGGGTAAGTACCAGGGTGCCATGATTGACTCCGACTAA SPRVHSLEEGKYQGAMIDSD* -1.633 0.000293 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24268 PSASASNSFIPTKNLTCRGP 20 SLAY-screened peptide P2618 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTGCGTCCGCGAGCAATAGTTTCATTCCCACGAAGAATCTTACCTGTAGGGGTCCGTAA PSASASNSFIPTKNLTCRGP* -1.633 2.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24269 SA 2 SLAY-screened peptide P2619 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGGCCTAGTGACCGACGCCCTTCCTTTTTGTCGTACCTACTTGAACACTACGTATGTCTAAC SA**PTPFLFVVPT*TLRMSN -1.633 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24270 LFGTLVLFSYYLDTHAR 17 SLAY-screened peptide P2620 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTTCGGTACCCTTGTCCTCTTTAGCTATTACCTTGATACGCATGCTCGGTAGCGCTACTAA LFGTLVLFSYYLDTHAR*RY* -1.633 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24271 RHSNSRSNNHSLGSSSNFVV 20 SLAY-screened peptide P2621 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCACTCTAATTCGCGCTCGAATAATCATTCCCTGGGGTCCTCGAGTAATTTTGTGGTTTAA RHSNSRSNNHSLGSSSNFVV* -1.633 0.000128 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24272 LLPKA 5 SLAY-screened peptide P2622 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCTTGCCGAAGGCCTAGATCTCCGAGTCTCTTAGGGCGTATGACGTCCTCTCTGACAGTTAA LLPKA*ISESLRAYDVLSDS* -1.633 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24273 YLNNITGPMVAKHTDTFYPA 20 SLAY-screened peptide P2623 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCTTAATAATATTACGGGGCCTATGGTTGCCAAGCACACTGATACTTTTTATCCCGCTTAA YLNNITGPMVAKHTDTFYPA* -1.632 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24274 HFTIY 5 SLAY-screened peptide P2624 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTTTACGATCTACTAGTCGGTCTATCTTACCCAGCGGCACCATGTTACGAGCTAGTCGTAA HFTIY*SVYLTQRHHVTS*S* -1.632 0.009444 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24275 CAPLCFAIHRYKASFQNLKD 20 SLAY-screened peptide P2625 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGCCCCCCTGTGCTTTGCCATTCATCGTTATAAGGCTTCGTTTCAGAATCTCAAGGACTAA CAPLCFAIHRYKASFQNLKD* -1.632 0.018012 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24276 RARPACAHAHTRKTASASIS 20 SLAY-screened peptide P2626 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCCCGTCCCGCGTGTGCTCACGCCCATACGCGCAAGACCGCGTCGGCGTCGATTTCCTAA RARPACAHAHTRKTASASIS* -1.631 0.015024 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24277 DTNMIASIWRNRDNLYKSED 20 SLAY-screened peptide P2627 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACACCAACATGATCGCCAGTATCTGGCGTAACCGTGACAACCTTTATAAGTCTGAGGATTAA DTNMIASIWRNRDNLYKSED* -1.631 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24278 PSRARVIVSVNINLYVYLFF 20 SLAY-screened peptide P2628 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCTCGGGCTCGCGTTATTGTTAGCGTCAATATCAATCTCTACGTTTACCTCTTTTTTTAA PSRARVIVSVNINLYVYLFF* -1.631 0.000653 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24279 PYDSKDPCSARNLTFLYHQL 20 SLAY-screened peptide P2629 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTATGACTCGAAGGACCCCTGCAGTGCCCGTAATCTGACTTTTCTTTATCACCAGCTCTAA PYDSKDPCSARNLTFLYHQL* -1.63 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24280 RIPLNSNVTRSDSSICTKCI 20 SLAY-screened peptide P2630 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCATTCCCCTCAATTCTAACGTCACCCGTTCCGACTCTAGTATTTGCACTAAGTGTATTTAA RIPLNSNVTRSDSSICTKCI* -1.629 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24281 TGPETNRPSRN 11 SLAY-screened peptide P2631 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGTCCCGAGACCAACCGCCCCTCCAGGAACTAGACCTGGTCTATGCTGTCGATGATCTAA TGPETNRPSRN*TWSMLSMI* -1.629 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24282 SLHRHFHIYTSSLPYYFFPT 20 SLAY-screened peptide P2632 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCTCCATAGGCATTTTCACATTTACACGAGCTCTTTGCCCTATTATTTTTTCCCCACTTAA SLHRHFHIYTSSLPYYFFPT* -1.629 0.002693 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24283 HSLSCRQYIVEPLYILNNSI 20 SLAY-screened peptide P2633 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTCCTTGAGTTGTCGTCAGTACATCGTGGAGCCGTTGTATATCCTGAATAATAGTATTTAA HSLSCRQYIVEPLYILNNSI* -1.629 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24284 RVEKPTLRSSVYFRTLLNQN 20 SLAY-screened peptide P2634 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGTGGAGAAGCCTACTCTGCGCAGCAGTGTCTACTTTAGGACGCTGTTGAACCAGAACTAA RVEKPTLRSSVYFRTLLNQN* -1.629 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24285 QPRTSRYFCPHITCPYGFYY 20 SLAY-screened peptide P2635 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCCCCGGACTAGTAGGTATTTTTGCCCCCATATTACTTGTCCCTATGGCTTTTACTACTAA QPRTSRYFCPHITCPYGFYY* -1.628 0.000141 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24286 ALQFLSSLADYCNNPPSGSA 20 SLAY-screened peptide P2636 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCTTCAGTTCTTGAGTTCCCTGGCCGACTATTGCAATAATCCCCCGAGTGGCTCCGCCTAA ALQFLSSLADYCNNPPSGSA* -1.628 0.031369 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24287 RTVLRTFHFPSSLQFSLCKS 20 SLAY-screened peptide P2637 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACCGTGCTTCGGACGTTTCATTTTCCTTCGTCTCTTCAGTTTTCGTTGTGTAAGTCGTAA RTVLRTFHFPSSLQFSLCKS* -1.628 0.000788 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24288 FG 2 SLAY-screened peptide P2638 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTGGGTAGGTGCCCTCTCCGTATTAGAGTACCCGTGTCATTCCTTATTCTCCCTTCCGTTAA FG*VPSPY*STRVIPYSPFR* -1.628 1.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24289 LNR 3 SLAY-screened peptide P2639 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAACCGTTGAAGAGCCTCCTGAATGTCCATTGTCATGTCACTAACTACCACCCGAACTAAC LNR*RAS*MSIVMSLTTTRTN -1.628 0.0441 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24290 RPCCSTARYRDLFA 14 SLAY-screened peptide P2640 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCCCTGTTGCTCCACTGCTCGCTACCGGGACCTGTTTGCTTAGTGCTCCATCCACTTCTAA RPCCSTARYRDLFA*CSIHF* -1.627 0.026413 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24291 HPYTPLCTDFWGSSMILVSS 20 SLAY-screened peptide P2641 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCCTTACACCCCTCTGTGTACGGACTTTTGGGGTTCGTCGATGATTCTCGTGTCCTCGTAA HPYTPLCTDFWGSSMILVSS* -1.627 0.013358 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24292 NTAWRHSTNFPSSSCPFVPL 20 SLAY-screened peptide P2642 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACACCGCCTGGCGCCACAGTACGAACTTTCCGTCCTCTTCTTGTCCGTTCGTTCCTCTTTAA NTAWRHSTNFPSSSCPFVPL* -1.627 0.045004 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24293 EPVISPSTTDKCCPNLFWYL 20 SLAY-screened peptide P2643 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGCCGGTCATCAGCCCTTCGACGACGGACAAGTGTTGCCCTAACCTCTTCTGGTACCTTTAA EPVISPSTTDKCCPNLFWYL* -1.627 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24294 YSPRSHS 7 SLAY-screened peptide P2644 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACAGCCCGCGCTCTCACTCCTAGACTTCCGCTTGTTATGCTCCTGCTAATCTTTGCTGCTAA YSPRSHS*TSACYAPANLCC* -1.627 0.000335 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24295 DPGWNYHFYSHRLQFHTNHS 20 SLAY-screened peptide P2645 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCCCGGTTGGAATTATCATTTCTATTCGCATCGCCTGCAGTTCCACACCAACCACTCTTAA DPGWNYHFYSHRLQFHTNHS* -1.627 0.027259 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24296 TLKCPLCIRRHE 12 SLAY-screened peptide P2646 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTTAAGTGCCCCCTCTGTATTCGGCGCCATGAGTAGAGCACCGATAACATCACTCCTTAA TLKCPLCIRRHE*STDNITP* -1.627 0.00369 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24297 VTIDDSILRRTVPKRGNFNV 20 SLAY-screened peptide P2647 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTACCATCGACGATTCGATTTTGCGGCGTACGGTCCCGAAGCGGGGTAACTTTAACGTTTAA VTIDDSILRRTVPKRGNFNV* -1.626 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24298 QGDLSSRALAIYFMPISRNG 20 SLAY-screened peptide P2648 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGGGGATCTCTCCTCGCGCGCCCTCGCTATCTACTTTATGCCGATTTCGAGGAATGGCTAA QGDLSSRALAIYFMPISRNG* -1.626 0.000439 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24299 RAYFTWPDGCRLLIT 15 SLAY-screened peptide P2649 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGCCTATTTCACGTGGCCCGATGGCTGCCGTCTCCTCATCACGTAGACGTCGAGCAAGTAA RAYFTWPDGCRLLIT*TSSK* -1.626 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24300 AAPTWHFSFYYQSSSTFSND 20 SLAY-screened peptide P2650 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGCTCCGACTTGGCACTTTTCTTTTTACTATCAGAGCTCGAGCACGTTTAGCAACGATTAA AAPTWHFSFYYQSSSTFSND* -1.625 0.001122 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24301 GDPSH 5 SLAY-screened peptide P2651 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTGACCCCTCCCACTAGTCCTGTCAGATTAGCACTACTATTAACGCTACTAGGAAGCCGTAA GDPSH*SCQISTTINATRKP* -1.625 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24302 PMTPRMNRKHYYTHLHADSS 20 SLAY-screened peptide P2652 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGATGACGCCTCGCATGAACAGGAAGCATTATTATACCCACCTGCATGCTGATTCGAGCTAA PMTPRMNRKHYYTHLHADSS* -1.624 0.005715 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24303 MYVPGSWSSPPYFFL 15 SLAY-screened peptide P2653 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGTACGTTCCCGGGTCTTGGTCATCACCCCCTTACTTCTTTCTTTGAGTCCCTCCAGCACTC MYVPGSWSSPPYFFL*VPPAL -1.624 0.002943 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24304 TNNDNGTNNLDGLVSVQCCP 20 SLAY-screened peptide P2654 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCAATAACGACAATGGTACTAATAACCTTGACGGCCTCGTTAGCGTGCAGTGTTGCCCTTAA TNNDNGTNNLDGLVSVQCCP* -1.624 0.001004 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24305 PKTTLAYSLVHRSRKCHLWS 20 SLAY-screened peptide P2655 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAAGACGACCTTGGCTTACTCCCTGGTTCATCGTAGTAGGAAGTGCCACTTGTGGAGTTAA PKTTLAYSLVHRSRKCHLWS* -1.624 0.002672 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24306 SSSPCNNSTRVARILPLYSL 20 SLAY-screened peptide P2656 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGAGTTCTCCGTGCAACAACAGTACCCGGGTTGCTCGTATTCTTCCGCTTTATTCCCTCTAA SSSPCNNSTRVARILPLYSL* -1.624 4.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24307 APSLSLLQRTVFSLSTCTSV 20 SLAY-screened peptide P2657 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCCTTCTCTGAGTCTTCTCCAGAGGACCGTGTTTTCTCTGAGCACCTGCACCAGTGTTTAA APSLSLLQRTVFSLSTCTSV* -1.624 0.032204 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24308 PLRDITRRIWRGLLMKVADF 20 SLAY-screened peptide P2658 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCCGCGATATTACGCGTCGCATTTGGCGGGGTCTCTTGATGAAGGTCGCTGACTTCTAA PLRDITRRIWRGLLMKVADF* -1.624 0.001333 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24309 RPSRSVRVQCPNFSKPNSYG 20 SLAY-screened peptide P2659 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCGTCGCGTTCTGTCAGGGTTCAGTGTCCGAATTTCTCTAAGCCTAATAGCTACGGTTAA RPSRSVRVQCPNFSKPNSYG* -1.624 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24310 MLLLIHLGPARTARPEYPKK 20 SLAY-screened peptide P2660 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCTGTTGCTTATCCATCTTGGCCCCGCTCGCACGGCGCGCCCCGAGTATCCGAAGAAGTAA MLLLIHLGPARTARPEYPKK* -1.624 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24311 CLHPIRGKMLPVPTWLAFTV 20 SLAY-screened peptide P2661 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTTCACCCTATTCGGGGCAAGATGCTGCCTGTCCCTACTTGGCTTGCTTTCACCGTCTAA CLHPIRGKMLPVPTWLAFTV* -1.623 0.002627 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24312 ENTSIRAHFTVHCSYLSTDY 20 SLAY-screened peptide P2662 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGAATACTAGTATTCGGGCTCATTTTACCGTCCATTGTTCGTACCTGTCTACTGACTACTAA ENTSIRAHFTVHCSYLSTDY* -1.623 0.000637 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24313 DAYGFPAPYAPLGLCTFHQL 20 SLAY-screened peptide P2663 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACGCGTACGGCTTTCCCGCGCCGTACGCCCCTCTGGGCCTCTGCACCTTCCATCAGCTGTAA DAYGFPAPYAPLGLCTFHQL* -1.623 0.003307 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24314 CARNVPHHRYPGPGPPRLPL 20 SLAY-screened peptide P2664 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGCCCGCAATGTGCCTCACCACCGGTATCCTGGCCCTGGGCCTCCTCGCCTGCCCCTCTAA CARNVPHHRYPGPGPPRLPL* -1.623 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24315 HVTVVYIAHSHLQSGNPPMD 20 SLAY-screened peptide P2665 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACGTTACGGTTGTTTACATTGCTCACAGCCATTTGCAGTCTGGTAACCCTCCTATGGACTAA HVTVVYIAHSHLQSGNPPMD* -1.622 0.007508 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24316 RPVFAHSRYIGAMSKIALLK 20 SLAY-screened peptide P2666 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCGGTCTTCGCGCACTCCCGTTATATCGGTGCCATGAGCAAGATTGCCCTTTTGAAGTAA RPVFAHSRYIGAMSKIALLK* -1.622 0.001513 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24317 SRALPASSNTTDPTTAMHSW 20 SLAY-screened peptide P2667 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCGTGCCCTCCCGGCGAGTTCTAACACTACCGATCCCACCACCGCGATGCACTCGTGGTAA SRALPASSNTTDPTTAMHSW* -1.622 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24318 PHLISQT 7 SLAY-screened peptide P2668 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCATCTTATTAGTCAGACGTAGCAGCAGTAGCCGAAGATGAGTAGTATTTTGCTTGACTAA PHLISQT*QQ*PKMSSILLD* -1.621 3.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24319 DFHCAPKINRFPEGYGTLQT 20 SLAY-screened peptide P2669 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACTTCCATTGTGCGCCGAAGATCAATCGGTTTCCGGAGGGCTATGGCACCCTGCAGACTTAA DFHCAPKINRFPEGYGTLQT* -1.621 0.002189 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24320 VR 2 SLAY-screened peptide P2670 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGCGTTAGATTACTCCCTCCGCTAGGTTTATTCGCACTATCGCGCGTCTGTTCTACTCCTAA VR*ITPSARFIRTIARLFYS* -1.621 1.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24321 PLERFCSCRHLDFYHSLAID 20 SLAY-screened peptide P2671 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTTGGAGCGTTTCTGTAGCTGCCGCCATTTGGATTTCTATCATTCGCTGGCGATCGACTAA PLERFCSCRHLDFYHSLAID* -1.621 7.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24322 FWVPHHGRCCIHHHYLILPR 20 SLAY-screened peptide P2672 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTGGGTTCCCCATCATGGTCGCTGTTGCATCCACCACCACTATCTTATCCTGCCGCGCTAA FWVPHHGRCCIHHHYLILPR* -1.621 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24323 CFRGHPLNVLGNLLIASHTR 20 SLAY-screened peptide P2673 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTTCCGCGGGCACCCTCTTAACGTGCTTGGTAATTTGCTTATTGCTAGCCACACGCGCTAA CFRGHPLNVLGNLLIASHTR* -1.62 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24324 PPCPFVKITSNTCSCDSANI 20 SLAY-screened peptide P2674 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTTGTCCCTTCGTGAAGATTACTTCGAACACTTGTAGTTGTGATAGCGCGAATATCTAA PPCPFVKITSNTCSCDSANI* -1.62 0.031151 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24325 PLPRICSGPPNN 12 SLAY-screened peptide P2675 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTGCCTCGCATTTGTTCTGGGCCCCCTAATAACTAGTCTACCACTCTTCTGTAGTACTAA PLPRICSGPPNN*STTLL*Y* -1.62 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24326 NTLAIVDIEPFVRGCDPAHA 20 SLAY-screened peptide P2676 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATACGCTCGCCATTGTCGATATCGAGCCCTTTGTGCGGGGGTGCGATCCGGCTCATGCTTAA NTLAIVDIEPFVRGCDPAHA* -1.62 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24327 LTHNSTSSNPNSITI 15 SLAY-screened peptide P2677 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGACGCATAATAGTACTTCCAGTAACCCTAACTCCATTACCATTTAGTGTTGTTTTATTTAA LTHNSTSSNPNSITI*CCFI* -1.619 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24328 CPRYPVMDVL 10 SLAY-screened peptide P2678 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCCGCGGTACCCCGTGATGGACGTGCTGTAGATCGGGCGTATGATGAGCCAGAACCACTAA CPRYPVMDVL*IGRMMSQNH* -1.619 0.000771 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24329 PYLSSGISALCPIGFLYSFV 20 SLAY-screened peptide P2679 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTATCTTTCTTCTGGGATCTCCGCCCTGTGTCCCATTGGGTTCCTGTACAGCTTTGTCTAA PYLSSGISALCPIGFLYSFV* -1.618 3.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24330 PPNIRCKSWTLNPRNFRLRP 20 SLAY-screened peptide P2680 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCCTAATATCAGGTGCAAGAGTTGGACTCTCAACCCTAGGAACTTTCGTCTTAGGCCCTAA PPNIRCKSWTLNPRNFRLRP* -1.618 0.002721 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24331 HPYFPLAMYIL 11 SLAY-screened peptide P2681 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCCGTACTTTCCGCTCGCCATGTACATTCTCTAGAATCTCTCTGGGATTAAGGCCACCTAA HPYFPLAMYIL*NLSGIKAT* -1.618 0.007115 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24332 QPGTSPSLANPTCYHSLKIY 20 SLAY-screened peptide P2682 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCCGGGGACCTCTCCGAGTTTGGCTAATCCGACCTGCTATCATTCGCTGAAGATTTATTAA QPGTSPSLANPTCYHSLKIY* -1.618 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24333 PKFTSHDPCAYSESNLFFKN 20 SLAY-screened peptide P2683 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGAAGTTTACCTCTCATGACCCCTGCGCCTACAGCGAGTCTAATCTTTTTTTTAAGAACTAA PKFTSHDPCAYSESNLFFKN* -1.618 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24334 RYIGTLNTYPDYHILPSYIL 20 SLAY-screened peptide P2684 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTATATCGGCACCCTTAATACCTACCCTGACTACCATATTCTGCCCTCGTACATTTTGTAA RYIGTLNTYPDYHILPSYIL* -1.618 0.010262 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24335 NVIPPYCFHYDYHHCCNRYH 20 SLAY-screened peptide P2685 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGTCATTCCTCCCTACTGTTTCCACTACGACTATCATCATTGTTGCAACCGCTACCATTAA NVIPPYCFHYDYHHCCNRYH* -1.618 0.010177 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24336 LWQFMCPCQLLQFPIWYAPM 20 SLAY-screened peptide P2686 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTGGCAGTTTATGTGCCCTTGCCAGCTTCTCCAGTTCCCTATCTGGTACGCCCCTATGTAA LWQFMCPCQLLQFPIWYAPM* -1.618 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24337 NPSCPVNCLSWSPLTFYFLI 20 SLAY-screened peptide P2687 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATCCCTCCTGCCCTGTGAACTGTCTTTCCTGGTCGCCTCTGACGTTCTACTTCTTGATTTAA NPSCPVNCLSWSPLTFYFLI* -1.617 0.000144 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24338 CPTFNYFHSAAPWMLPVASTS 21 SLAY-screened peptide P2688 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCCACTTTTAACTACTTTCATTCCGCCGCCCCGTGGATGCTCCCCGTCGCGAGCACGAGC CPTFNYFHSAAPWMLPVASTS -1.617 0.000237 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24339 LLSQSLRGPGNEPIHLLSVC 20 SLAY-screened peptide P2689 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTTGTCGCAGAGCCTGCGCGGTCCTGGCAACGAGCCTATTCACTTGCTTAGCGTGTGCTAA LLSQSLRGPGNEPIHLLSVC* -1.617 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24340 AASHVYSMDPSWFHTYPLLS 20 SLAY-screened peptide P2690 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGCTTCCCATGTTTACTCTATGGATCCGTCCTGGTTCCACACTTATCCTTTGCTCTCCTAA AASHVYSMDPSWFHTYPLLS* -1.617 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24341 RSVG 4 SLAY-screened peptide P2691 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTTCGGTTGGCTAGGCGCCCGGTTCCTATAAGAACTCGATGTTCATATCTATGCGAGTTAAC RSVG*APGSYKNSMFISMRVN -1.616 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24342 LLDKLLHGFCNLRGHNR 17 SLAY-screened peptide P2692 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCTTGATAAGTTGTTGCACGGGTTCTGTAATCTCCGCGGGCATAATCGCTAGTAGAATTAA LLDKLLHGFCNLRGHNR**N* -1.616 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24343 SYFRSNVYNTVPTSRRCPLP 20 SLAY-screened peptide P2693 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCTACTTCCGCTCTAACGTCTATAACACGGTCCCGACGAGTAGGCGCTGTCCCCTCCCCTAA SYFRSNVYNTVPTSRRCPLP* -1.616 0.004191 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24344 GSFTAHNHVNICSNRPLLNF 20 SLAY-screened peptide P2694 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTCTTTTACCGCCCACAATCATGTTAATATTTGCAGTAATAGGCCGCTGCTCAATTTTTAA GSFTAHNHVNICSNRPLLNF* -1.616 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24345 LNTDGSLLRPV 11 SLAY-screened peptide P2695 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAATACGGACGGCTCTCTGCTTAGGCCTGTTTAGGGTATCATCCACCCTGTTTTGTTCTAA LNTDGSLLRPV*GIIHPVLF* -1.615 1.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24346 TN 2 SLAY-screened peptide P2696 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCAATTAGTTTAGTTACTGTCATAATCATCCTATCTACGTCAGTAATTTCCAGAACCACTAA TN*FSYCHNHPIYVSNFQNH* -1.615 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24347 RSRARDPPTQRRGPGDTLYS 20 SLAY-screened peptide P2697 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTCGCGGGCGCGTGACCCGCCCACGCAGCGTAGGGGCCCCGGCGATACTCTGTATTCGTAA RSRARDPPTQRRGPGDTLYS* -1.615 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24348 PLSSQRYSDSHLGPNHPRPT 20 SLAY-screened peptide P2698 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTTAGCAGTCAGCGGTATTCCGACTCTCACTTGGGCCCCAATCATCCTCGCCCTACTTAA PLSSQRYSDSHLGPNHPRPT* -1.615 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24349 RNCVTRYTIRPNIKYMGPFK 20 SLAY-screened peptide P2699 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCAATTGCGTCACCCGGTATACTATCCGTCCCAACATCAAGTATATGGGCCCCTTCAAGTAA RNCVTRYTIRPNIKYMGPFK* -1.615 2.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24350 TGYRFYTYNTPLHH 14 SLAY-screened peptide P2700 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGGTTACCGCTTTTACACTTACAACACTCCGCTTCATCACTAGTACCTGACGTACGGCTAA TGYRFYTYNTPLHH*YLTYG* -1.614 9.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24351 IDAAHYVRFDTYTYDDISSD 20 SLAY-screened peptide P2701 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGATGCGGCGCACTATGTCCGCTTTGACACGTATACTTACGACGATATTTCTTCGGACTAA IDAAHYVRFDTYTYDDISSD* -1.614 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24352 CLTYYAQ 7 SLAY-screened peptide P2702 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTCACGTACTACGCCCAGTAGATCCATCTTAATAGCCGCGCTGCGACCGCCGCCAACTAA CLTYYAQ*IHLNSRAATAAN* -1.614 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24353 SRPTSRFLEGHSRSMTEPAS 20 SLAY-screened peptide P2703 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCCGTCCTACCTCCCGCTTTCTGGAGGGCCATTCGCGGTCCATGACGGAGCCCGCGTCTTAA SRPTSRFLEGHSRSMTEPAS* -1.614 0.00306 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24354 KKAPICDRTPLTSPFYTWMV 20 SLAY-screened peptide P2704 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGAAGGCGCCCATTTGTGACCGCACCCCGCTGACGAGCCCGTTCTACACTTGGATGGTTTAA KKAPICDRTPLTSPFYTWMV* -1.613 0.000592 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24355 PPLFTRVTTSVPE 13 SLAY-screened peptide P2705 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCTCTCTTTACTCGTGTCACCACCTCGGTTCCTGAGTAGCATTCTTATGCTCACCCGTAA PPLFTRVTTSVPE*HSYAHP* -1.613 0.028453 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24356 HMPPNNASVDLHNSSSIIEM 20 SLAY-screened peptide P2706 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATATGCCCCCGAATAACGCGTCGGTTGATCTTCATAATTCCAGCTCTATCATCGAGATGTAA HMPPNNASVDLHNSSSIIEM* -1.613 0.02047 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24357 SNCRIRTVNIH 11 SLAY-screened peptide P2707 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAACTGTCGGATTCGTACCGTCAATATCCATTAGGCCTTCAGTCGCAGTAAGTCGGTTTAA SNCRIRTVNIH*AFSRSKSV* -1.613 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24358 RHNRDVIPYSPAPCLPMYNN 20 SLAY-screened peptide P2708 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCACAACCGTGATGTCATCCCCTACTCTCCGGCTCCGTGCCTTCCCATGTATAACAATTAA RHNRDVIPYSPAPCLPMYNN* -1.613 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24359 AVPPPHTLDDHHFNNSKTFS 20 SLAY-screened peptide P2709 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCGTCCCTCCGCCTCATACTCTTGATGATCATCACTTTAACAACAGTAAGACCTTTTCTTAA AVPPPHTLDDHHFNNSKTFS* -1.613 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24360 VQSLDVQYMAICH 13 SLAY-screened peptide P2710 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCAGTCTCTTGATGTGCAGTACATGGCTATTTGTCATTAGTAGGGGACCGCTTAGGTCTAA VQSLDVQYMAICH**GTA*V* -1.613 0.001558 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24361 SAHIISSIE 9 SLAY-screened peptide P2711 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGCCCATATTATTTCTTCCATTGAGTAGGACCAGCAGTCCATCGAGAGCTTTAAGAGGTAA SAHIISSIE*DQQSIESFKR* -1.613 0.016074 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24362 SRQPQYYYGYIFMSPGRISN 20 SLAY-screened peptide P2712 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTCGGCAGCCCCAGTACTATTACGGGTATATTTTCATGTCTCCCGGGCGGATTTCCAACTAA SRQPQYYYGYIFMSPGRISN* -1.612 0.000422 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24363 QGRILNKGIIPLLPCLAMTGN 21 SLAY-screened peptide P2713 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGGTCGCATCTTGAACAAGGGAATTATCCCTCTTTTACCCTGCCTAGCAATGACTGGTAAC QGRILNKGIIPLLPCLAMTGN -1.612 0.000363 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24364 FWVMRHRSNEGLGSDQTGSI 20 SLAY-screened peptide P2714 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTGGGTCATGAGGCATAGGTCGAACGAGGGTCTGGGCTCCGATCAGACCGGGAGCATTTAA FWVMRHRSNEGLGSDQTGSI* -1.612 0.021618 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24365 TPYYCAPLSTFKVTELDITI 20 SLAY-screened peptide P2715 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCCCTATTACTGCGCCCCCCTGTCGACCTTTAAGGTTACCGAGCTGGACATCACGATTTAA TPYYCAPLSTFKVTELDITI* -1.612 7e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24366 VDRFPWNAKLYCGSAIPHNI 20 SLAY-screened peptide P2716 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGACCGTTTTCCCTGGAATGCGAAGTTGTATTGTGGCTCGGCCATTCCGCATAATATCTAA VDRFPWNAKLYCGSAIPHNI* -1.612 0.000461 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24367 SFFHASLTEHYCTHQLSLAQ 20 SLAY-screened peptide P2717 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGTTCTTCCACGCCAGCCTTACTGAGCATTACTGTACTCACCAGCTGTCCCTCGCCCAGTAA SFFHASLTEHYCTHQLSLAQ* -1.612 0.001974 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24368 SMYFNARLGRAVFSSHPLDN 20 SLAY-screened peptide P2718 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTATGTACTTTAACGCCCGCCTGGGTCGGGCCGTGTTCAGCAGCCATCCGTTGGATAATTAA SMYFNARLGRAVFSSHPLDN* -1.612 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24369 FIFSIVYARRNLPKRLPSTS 20 SLAY-screened peptide P2719 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTATTTTCTCTATTGTCTATGCCCGGCGGAATCTTCCGAAGCGTTTGCCCTCCACTAGTTAA FIFSIVYARRNLPKRLPSTS* -1.612 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24370 PCYPNIMLLLLLSLIRSYWL 20 SLAY-screened peptide P2720 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTGCTATCCCAATATCATGTTGCTTCTGCTCCTGAGTCTGATTCGCTCCTATTGGCTTTAA PCYPNIMLLLLLSLIRSYWL* -1.612 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24371 PSRYTAASAPPPSIRSTHAY 20 SLAY-screened peptide P2721 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCAGTAGGTACACGGCCGCTTCGGCTCCTCCGCCCAGCATCCGTTCGACCCACGCCTATTAA PSRYTAASAPPPSIRSTHAY* -1.611 0.006705 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24372 MTSSFTLPSVICNQLPSPPI 20 SLAY-screened peptide P2722 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGACCAGCAGTTTCACTCTCCCTTCGGTTATTTGTAATCAGCTTCCTTCTCCGCCGATCTAA MTSSFTLPSVICNQLPSPPI* -1.611 1.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24373 HRPDKINNNETYTELSTTRN 20 SLAY-screened peptide P2723 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCGCCCTGACAAGATCAATAACAATGAGACTTATACTGAGTTGTCCACCACTAGGAATTAA HRPDKINNNETYTELSTTRN* -1.611 2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24374 NCSYHLTVR 9 SLAY-screened peptide P2724 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTGCAGTTACCACCTCACGGTTCGCTAGGACACCAATGGCCTTTACGTTACGTGCGACTAA NCSYHLTVR*DTNGLYVTCD* -1.611 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24375 LPFAHACPTDLPAMHLTT 18 SLAY-screened peptide P2725 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCGTTTGCTCATGCCTGCCCGACTGATCTTCCCGCTATGCATTTGACCACCTAGGGCTAA LPFAHACPTDLPAMHLTT*G* -1.611 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24376 PLYIFARYPDIADTCKIPPM 20 SLAY-screened peptide P2726 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGCTCTACATCTTTGCGCGCTACCCGGATATCGCCGATACCTGCAAGATCCCGCCGATGTAA PLYIFARYPDIADTCKIPPM* -1.611 1.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24377 SDLRNTLFWYMTLCGPYALF 20 SLAY-screened peptide P2727 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCGACTTGCGCAACACCCTGTTTTGGTACATGACGCTGTGCGGTCCCTATGCGCTTTTCTAA SDLRNTLFWYMTLCGPYALF* -1.611 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24378 TSRNAVANAVGDLATETSII 20 SLAY-screened peptide P2728 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGTCCCGTAACGCTGTGGCGAATGCGGTGGGTGATCTGGCCACCGAGACTTCCATTATCTAA TSRNAVANAVGDLATETSII* -1.61 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24379 RFYQQTPPTVGHLPYCTSIV 20 SLAY-screened peptide P2729 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCTTCTACCAGCAGACGCCGCCTACGGTGGGCCACTTGCCTTATTGCACCAGTATCGTTTAA RFYQQTPPTVGHLPYCTSIV* -1.61 5.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24380 RPECYVFHPVKLAVARLPRT 20 SLAY-screened peptide P2730 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCCGGAGTGCTATGTCTTCCACCCGGTGAAGCTGGCTGTCGCCCGCCTTCCCCGGACCTAA RPECYVFHPVKLAVARLPRT* -1.61 0.002824 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24381 PATTHYPHSVRCLDIHNFPH 20 SLAY-screened peptide P2731 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGCGACGACCCATTACCCCCACAGTGTGCGTTGCTTGGATATCCATAATTTCCCGCATTAA PATTHYPHSVRCLDIHNFPH* -1.609 0.043794 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24382 KPGSSILGHSSIPSFLIPTT 20 SLAY-screened peptide P2732 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCCGGGTAGTTCTATTCTTGGTCATTCGAGTATCCCCTCTTTTTTGATTCCGACGACGTAA KPGSSILGHSSIPSFLIPTT* -1.609 0.000277 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24383 PSISSGPYGSPHNLARFYFR 20 SLAY-screened peptide P2733 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTCTATCTCTTCGGGCCCTTATGGTAGTCCTCATAATCTTGCCCGCTTTTATTTCCGGTAA PSISSGPYGSPHNLARFYFR* -1.609 0.001291 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24384 CALYTYRFFHNHFNACFMYT 20 SLAY-screened peptide P2734 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGCCCTGTACACTTATCGCTTCTTTCATAACCATTTCAATGCCTGTTTCATGTATACCTAA CALYTYRFFHNHFNACFMYT* -1.609 0.001275 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24385 PIKQSCFARPIAVTNTSHNL 20 SLAY-screened peptide P2735 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGATCAAGCAGTCCTGTTTTGCCCGCCCCATCGCCGTTACGAATACGAGTCACAATCTGTAA PIKQSCFARPIAVTNTSHNL* -1.608 0.000315 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24386 HWDPLPSNPPASTPDARHAY 20 SLAY-screened peptide P2736 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTGGGATCCTCTCCCCAGTAATCCGCCTGCTAGTACTCCCGACGCGCGTCACGCGTATTAA HWDPLPSNPPASTPDARHAY* -1.608 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24387 TARNRYHATTITHYGARKNC 20 SLAY-screened peptide P2737 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGCCAGGAACCGTTACCATGCGACTACTATTACTCACTATGGGGCCCGTAAGAATTGCTAA TARNRYHATTITHYGARKNC* -1.608 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24388 NWD 3 SLAY-screened peptide P2738 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTGGGACTAGAAGAGTGCTCCCCACTGCTACAGCACTTCGGATCGCCATACTATCACCTAA NWD*KSAPHCYSTSDRHTIT* -1.608 0.000794 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24389 LTRPPNDRY 9 SLAY-screened peptide P2739 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTACCCGGCCTCCCAACGACCGCTACTAGGAGACTATGGATCCCAAGCGCAACCAGGACTAA LTRPPNDRY*ETMDPKRNQD* -1.608 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24390 DPTHDKDDGLLCAPSTIRFL 20 SLAY-screened peptide P2740 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCCGACCCATGACAAGGATGACGGCCTCTTGTGCGCCCCTTCTACCATCCGTTTCCTCTAA DPTHDKDDGLLCAPSTIRFL* -1.608 0.010076 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24391 RLIVTVSN 8 SLAY-screened peptide P2741 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCCTGATCGTTACGGTGAGCAATTAGTTCAACTTTGTGAAGGGGAGTAAGGCTGCCGCTTAA RLIVTVSN*FNFVKGSKAAA* -1.607 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24392 YVCVARNY 8 SLAY-screened peptide P2742 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGTTTGTGTTGCCCGTAATTACTAGTCTACTCCGCTTCTCCGCCCCATTATGACGGCGTAA YVCVARNY*STPLLRPIMTA* -1.607 0.000808 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24393 RPGRDSMFDD 10 SLAY-screened peptide P2743 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGCCCGGCCGCGATTCCATGTTTGACGACTAGATTGACGTGTCTATCGCTCACGTTGGTAAC RPGRDSMFDD*IDVSIAHVGN -1.607 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24394 VLSS 4 SLAY-screened peptide P2744 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCTTAGCAGTTAGCTCACTGTTCGATTGGTTGTACGACCCGTGCGAACAGCGATAGTTAAC VLSS*LTVRLVVRPVRTAIVN -1.607 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24395 DC 2 SLAY-screened peptide P2745 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGATTGCTAGAGCCACCTTTGCAATTCGAATTCCGTTTCTAGGGAGCATAGCAATTGTGAGTAA DC*SHLCNSNSVSREHSNCE* -1.607 0.000417 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24396 GVQTPCQLINEYLCCVTIHI 20 SLAY-screened peptide P2746 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTGTGCAGACGCCCTGTCAGCTCATCAACGAGTACCTGTGTTGTGTGACCATTCACATTTAA GVQTPCQLINEYLCCVTIHI* -1.606 1.1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24397 SNFPRAADHTLIT 13 SLAY-screened peptide P2747 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCAACTTTCCTAGGGCGGCTGACCACACTCTCATTACGTAGACTAGCTATTTGAAGCGCTAA SNFPRAADHTLIT*TSYLKR* -1.606 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24398 LAYVGFFICFLFTRRDADCT 20 SLAY-screened peptide P2748 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGCTTATGTCGGTTTTTTCATTTGTTTCCTGTTTACCCGCCGGGACGCCGACTGTACCTAA LAYVGFFICFLFTRRDADCT* -1.606 3.6e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24399 TVAT 4 SLAY-screened peptide P2749 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGTTGCTACTTAGTCGGTGGCGCTTTGCATGCAGATGACTCATGCCGCCGATAACACGTAA TVAT*SVALCMQMTHAADNT* -1.606 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24400 HTSPCPCKYYSLICFSIISN 20 SLAY-screened peptide P2750 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATACGTCTCCTTGTCCTTGTAAGTATTATAGTCTTATCTGTTTTTCCATTATCTCCAACTAA HTSPCPCKYYSLICFSIISN* -1.606 3.4e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24401 PGLPCVLVYYNMIFTLPCAF 20 SLAY-screened peptide P2751 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGGGCTCCCTTGTGTCCTTGTGTATTACAATATGATTTTCACTTTGCCCTGCGCCTTTTAA PGLPCVLVYYNMIFTLPCAF* -1.605 0.002646 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24402 CHVFDIYMRRYDTLCFVYYS 20 SLAY-screened peptide P2752 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCACGTCTTTGACATTTACATGCGCCGTTATGACACGCTCTGCTTCGTTTACTATAGTTAA CHVFDIYMRRYDTLCFVYYS* -1.605 2.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24403 AVSRMFNNDTLDCHF 15 SLAY-screened peptide P2753 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGTCAGCCGTATGTTCAATAACGATACCCTTGACTGTCATTTCTAGACGGATACTGATTAA AVSRMFNNDTLDCHF*TDTD* -1.605 0.0039 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24404 CRARSYWGPFYLLDNVTSQY 20 SLAY-screened peptide P2754 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGCGCCCGTAGCTATTGGGGCCCGTTTTATTTGCTTGACAACGTTACGTCCCAGTATTAA CRARSYWGPFYLLDNVTSQY* -1.605 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24405 AGTRAAGTSDDISYSPIECL 20 SLAY-screened peptide P2755 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGGGTACCCGTGCGGCCGGTACCTCGGATGATATTAGTTACAGCCCTATTGAGTGCCTCTAA AGTRAAGTSDDISYSPIECL* -1.604 0.018948 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24406 PIKASPMTNYWATSNPLDDI 20 SLAY-screened peptide P2756 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGATTAAGGCGTCCCCTATGACGAATTACTGGGCCACCAGCAATCCCCTTGATGACATCTAA PIKASPMTNYWATSNPLDDI* -1.604 1.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24407 HSPTLYPRGDTNCRPPAASV 20 SLAY-screened peptide P2757 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATAGCCCGACCCTGTATCCGCGCGGTGACACTAATTGTCGGCCGCCCGCTGCCAGCGTGTAA HSPTLYPRGDTNCRPPAASV* -1.604 0.001656 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24408 CESTSGFERFFYISSNNPVI 20 SLAY-screened peptide P2758 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGAGTCCACCTCTGGCTTTGAGCGGTTCTTTTACATTTCTTCCAATAACCCCGTTATCTAA CESTSGFERFFYISSNNPVI* -1.604 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24409 RSIFSVERLISNKPHHLQIF 20 SLAY-screened peptide P2759 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGGTCTATCTTTTCGGTGGAGCGGCTGATCTCCAATAAGCCTCACCACCTTCAGATTTTCTAA RSIFSVERLISNKPHHLQIF* -1.603 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24410 HCFHLMASNPTTSVAPSRRS 20 SLAY-screened peptide P2760 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTGTTTTCATCTTATGGCCTCCAACCCTACCACCAGCGTTGCGCCTTCTAGGCGGTCCTAA HCFHLMASNPTTSVAPSRRS* -1.602 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24411 CSAPRDKPYEPHPANSPYTN 20 SLAY-screened peptide P2761 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCAGCGCGCCGCGCGACAAGCCTTACGAGCCGCATCCGGCCAACTCCCCTTATACGAACTAA CSAPRDKPYEPHPANSPYTN* -1.601 0.035271 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24412 IVINCNFVNI 10 SLAY-screened peptide P2762 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTGTTATTAATTGTAACTTCGTTAATATCTAGATGGCCACTTGGCAGTAGTTCAGTACTTAA IVINCNFVNI*MATWQ*FST* -1.601 0.000559 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24413 AEGINKYLGTK 11 SLAY-screened peptide P2763 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTGAGGGGATCAACAAGTATCTCGGTACCAAGTAGTACACTCGTGTTTGTGCCGAGCACTAA AEGINKYLGTK*YTRVCAEH* -1.601 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24414 PPANMSS 7 SLAY-screened peptide P2764 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCCCGCCAATATGTCTAGCTAGGTTTCTGATTTTCTGTCCGGTCGGGCGCTGCGGAGGTAA PPANMSS*VSDFLSGRALRR* -1.6 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24415 PHNSSFRPRIRESSGFGNLS 20 SLAY-screened peptide P2765 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCATAACTCCTCCTTCCGGCCGCGTATCCGCGAGTCCAGCGGCTTTGGCAATCTGTCTTAA PHNSSFRPRIRESSGFGNLS* -1.6 0.00276 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24416 VRMCAATWYMPS 12 SLAY-screened peptide P2766 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCGTATGTGCGCTGCGACTTGGTACATGCCCAGTTAGATTCACGTGATGGTCATCCGGTAA VRMCAATWYMPS*IHVMVIR* -1.6 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24417 LMPTLSLIRCGTVLNRQPPS 20 SLAY-screened peptide P2767 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGATGCCCACCCTTAGTCTCATTCGCTGCGGGACTGTGCTCAATCGGCAGCCCCCTAGTTAA LMPTLSLIRCGTVLNRQPPS* -1.6 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24418 DRADFSCDHYSYHFLHYTSI 20 SLAY-screened peptide P2768 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCGTGCCGACTTTTCCTGCGACCATTACAGTTACCATTTTCTGCACTACACTTCCATCTAA DRADFSCDHYSYHFLHYTSI* -1.6 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24419 EAVGVYMNPYSLRPCELYLI 20 SLAY-screened peptide P2769 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGCTGTCGGCGTCTACATGAATCCGTATAGTTTGCGTCCTTGTGAGCTTTATCTCATCTAA EAVGVYMNPYSLRPCELYLI* -1.6 0.026238 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24420 NVHNDMGN 8 SLAY-screened peptide P2770 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACGTCCACAACGACATGGGTAACTAGCTGCTTTTGTACACTAAGAACACTTAGGATCTGTAA NVHNDMGN*LLLYTKNT*DL* -1.6 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24421 SRHMCANAVDIMPLGSNVHR 20 SLAY-screened peptide P2771 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCGGCATATGTGCGCTAACGCTGTTGATATCATGCCTCTCGGGAGTAATGTGCATCGGTAA SRHMCANAVDIMPLGSNVHR* -1.599 0.001738 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24422 SNTSSHGIPMRCMSIMRLASN 21 SLAY-screened peptide P2772 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAACACGAGTTCTCATGGGATTCCTATGCGCTGTATGTCTATTATGAGATTAGCATCTAAC SNTSSHGIPMRCMSIMRLASN -1.599 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24423 EVLPVEPPTRHIGNCKTSQY 20 SLAY-screened peptide P2773 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGGTGTTGCCTGTGGAGCCTCCTACTCGGCACATTGGGAATTGTAAGACTAGCCAGTACTAA EVLPVEPPTRHIGNCKTSQY* -1.599 0.010247 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24424 CYIGLTWIRISPLCYLSNTH 20 SLAY-screened peptide P2774 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTATATTGGCCTGACCTGGATTAGGATTAGTCCTCTCTGCTATCTGTCGAACACTCATTAA CYIGLTWIRISPLCYLSNTH* -1.599 0.018239 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24425 MHFISLRRTPSCPVPNNFPH 20 SLAY-screened peptide P2775 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCACTTCATCTCCTTGCGCCGCACTCCGTCGTGCCCTGTGCCTAACAATTTTCCCCATTAA MHFISLRRTPSCPVPNNFPH* -1.599 0.034069 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24426 PTSTNEKLTWNRNIPPHRRN 20 SLAY-screened peptide P2776 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGACGAGCACCAATGAGAAGCTGACGTGGAACCGTAACATTCCTCCTCACCGTAGGAACTAA PTSTNEKLTWNRNIPPHRRN* -1.599 0.033001 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24427 ATNAYNVSHSGNRSLFGDEV 20 SLAY-screened peptide P2777 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCTACGAATGCTTACAATGTCAGTCATAGTGGCAACCGCTCCTTGTTCGGGGATGAGGTTTAA ATNAYNVSHSGNRSLFGDEV* -1.598 0.003019 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24428 CCHTDILIPYAHSTWYHDHH 20 SLAY-screened peptide P2778 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTGCCACACCGACATTCTCATTCCCTATGCTCATTCTACGTGGTACCATGACCATCACTAA CCHTDILIPYAHSTWYHDHH* -1.598 0.033525 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24429 IRNRSHCYLSLSDHSDLPCT 20 SLAY-screened peptide P2779 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCGTAACCGTTCCCACTGCTACCTTTCTCTTTCTGACCACAGCGACTTGCCGTGTACCTAA IRNRSHCYLSLSDHSDLPCT* -1.598 0.002458 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24430 FGA 3 SLAY-screened peptide P2780 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGGTGCCTAGCGTTGCGCCAATAGCATTATCCGCCCTACTCTCCTGCGGACGAGCCATTAA FGA*RCANSIIRPTLLRTSH* -1.598 0.028784 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24431 EIDDRRGFNSLNQPNHKSRS 20 SLAY-screened peptide P2781 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGATTGATGATCGCCGTGGGTTTAATTCGCTCAACCAGCCGAATCACAAGTCCCGTTCCTAA EIDDRRGFNSLNQPNHKSRS* -1.598 4e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24432 IIKAFLETMYNRNMAHCNPA 20 SLAY-screened peptide P2782 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTATTAAGGCCTTCCTTGAGACTATGTACAATCGGAATATGGCTCATTGCAACCCTGCTTAA IIKAFLETMYNRNMAHCNPA* -1.598 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24433 RACINASTTYCTKSS 15 SLAY-screened peptide P2783 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCGCTTGCATTAATGCGTCGACTACTTACTGTACCAAGTCGTCGTAGTTCCATTATTCTTAA RACINASTTYCTKSS*FHYS* -1.597 0.00137 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24434 PSACINPNQN 10 SLAY-screened peptide P2784 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCCGCCTGTATTAATCCTAATCAGAATTAGGCTACCAATTGCCGGTTCAAGGGTGTGTAA PSACINPNQN*ATNCRFKGV* -1.597 0.000298 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24435 LL 2 SLAY-screened peptide P2785 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGCTTTAGCATAGGCCGCCCATCGTCGTTGTAATGCTGTTTATTGTAGGTCCAAGATCTAAC LL*HRPPIVVVMLFIVGPRSN -1.597 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24436 LCPTSTVDRHYDYNVPMWNE 20 SLAY-screened peptide P2786 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTGCCCGACGTCGACCGTTGATAGGCACTATGACTACAATGTCCCTATGTGGAACGAGTAA LCPTSTVDRHYDYNVPMWNE* -1.596 0.006591 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24437 RCSPVFIYFPHFDAVSNVLV 20 SLAY-screened peptide P2787 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTGTAGCCCCGTGTTCATTTATTTTCCCCATTTCGACGCGGTTTCTAACGTTCTCGTTTAA RCSPVFIYFPHFDAVSNVLV* -1.596 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24438 HYMFPTVFARSNISHPHPPN 20 SLAY-screened peptide P2788 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACTACATGTTCCCCACTGTCTTTGCCCGTTCCAATATTTCGCATCCCCATCCTCCGAACTAA HYMFPTVFARSNISHPHPPN* -1.596 0.016863 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24439 TKANANLYPPPNITLPMYMR 20 SLAY-screened peptide P2789 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGAAGGCCAATGCTAATCTCTACCCTCCTCCTAATATTACGTTGCCCATGTACATGCGCTAA TKANANLYPPPNITLPMYMR* -1.596 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24440 TVRTPTNCVHRLACPAV 17 SLAY-screened peptide P2790 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTTCGTACCCCCACTAATTGTGTCCATCGTTTGGCTTGCCCGGCTGTTTAGAGTACTTAA TVRTPTNCVHRLACPAV*ST* -1.595 0.003792 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24441 YA 2 SLAY-screened peptide P2791 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACGCCTAGACGCAGTTGCTTACGTGTACCTCTAGCCATACCACCGAGAACGCTAAGTACTAA YA*TQLLTCTSSHTTENAKY* -1.595 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24442 PVIIIIHESGNV 12 SLAY-screened peptide P2792 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTGTTATCATCATTATTCACGAGTCCGGTAATGTTTAGGCCTGCACGGTCTATGTTTCCTAA PVIIIIHESGNV*ACTVYVS* -1.595 0.000979 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24443 FTHYHI 6 SLAY-screened peptide P2793 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTACTCACTACCATATTTAGAAGACCTCTTCTTTTGAGCCCCGTCTCGGGACGAGTAACTAA FTHYHI*KTSSFEPRLGTSN* -1.595 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24444 IWHRILMSLSPAARLLRGRSN 21 SLAY-screened peptide P2794 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCTGGCATCGAATACTCATGAGCTTAAGCCCAGCCGCACGACTACTGCGCGGTCGGTCTAAC IWHRILMSLSPAARLLRGRSN -1.595 0.024526 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24445 PSWGCWDHFHAWANLPINIT 20 SLAY-screened peptide P2795 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTTCCTGGGGCTGTTGGGATCACTTCCATGCTTGGGCTAACTTGCCTATCAATATCACCTAA PSWGCWDHFHAWANLPINIT* -1.595 0.010987 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24446 TLKCQRYQFVQIRKLSNPYI 20 SLAY-screened peptide P2796 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTTGAAGTGCCAGCGGTATCAGTTCGTCCAGATCAGGAAGTTGAGCAACCCTTACATTTAA TLKCQRYQFVQIRKLSNPYI* -1.595 0.006175 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24447 LTWRPSFRNRFLWNVMDPCR 20 SLAY-screened peptide P2797 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCACCTGGCGCCCCTCGTTCCGCAACCGCTTCCTGTGGAACGTCATGGACCCTTGTCGCTAA LTWRPSFRNRFLWNVMDPCR* -1.594 0.000106 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24448 LVQLPTPLITRGLFHLNL 18 SLAY-screened peptide P2798 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGTCCAGCTCCCGACTCCGCTTATCACTCGTGGCCTGTTTCACCTCAACCTGTAGGCGTAA LVQLPTPLITRGLFHLNL*A* -1.594 0.000516 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24449 LKSRRAPCVSIYPRCRYYYF 20 SLAY-screened peptide P2799 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAAGTCTCGCCGCGCGCCCTGCGTCTCCATCTACCCTCGGTGTCGCTATTATTACTTCTAA LKSRRAPCVSIYPRCRYYYF* -1.594 0.008347 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24450 PALPMMLRNYYTVMMECHMM 20 SLAY-screened peptide P2800 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGCCCTCCCTATGATGCTCCGTAATTACTATACTGTCATGATGGAGTGTCACATGATGTAA PALPMMLRNYYTVMMECHMM* -1.594 0.036681 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24451 FLNNLNKNCPSNRSHNAWPY 20 SLAY-screened peptide P2801 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTCTTAATAATCTTAATAAGAATTGTCCTTCGAATCGTTCTCATAATGCTTGGCCTTATTAA FLNNLNKNCPSNRSHNAWPY* -1.594 0.004408 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24452 IQGLSTLKV 9 SLAY-screened peptide P2802 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTCAGGGTCTGTCGACTCTTAAGGTCTAGCCGTACCGCCTTGCGGGCGCCGCCTATTGTTAA IQGLSTLKV*PYRLAGAAYC* -1.593 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24453 SCGITTNRRAHITDSHARWN 20 SLAY-screened peptide P2803 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTGCGGTATTACGACCAACAGGCGTGCTCATATTACGGATAGCCATGCCAGGTGGAATTAA SCGITTNRRAHITDSHARWN* -1.593 0.007256 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24454 LPLRSCHQL 9 SLAY-screened peptide P2804 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGCCGCTTCGCAGCTGTCATCAGCTTTAGATCCTCAACACCCTTAACCTTACCTCCAACTAA LPLRSCHQL*ILNTLNLTSN* -1.592 0.002019 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24455 ENHMDRADLTDVPSFTCGLL 20 SLAY-screened peptide P2805 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGAGAACCATATGGACCGGGCCGATCTCACCGACGTTCCCTCTTTTACGTGCGGCTTGCTGTAA ENHMDRADLTDVPSFTCGLL* -1.592 0.030523 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24456 GYPG 4 SLAY-screened peptide P2806 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCTATCCGGGCTAGTTCTCCTATACGCTTTCCGTTCAGTGTATTCTTAGCGTTGGGATCTAA GYPG*FSYTLSVQCILSVGI* -1.592 0.000312 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24457 VAHYTPSPFTYLPASTMYTN 20 SLAY-screened peptide P2807 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTGCGCACTACACTCCGTCGCCTTTTACTTACCTGCCGGCTAGTACTATGTATACCAATTAA VAHYTPSPFTYLPASTMYTN* -1.591 0.006409 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24458 PQRGRAFGLRMPSMLAPSVFN 21 SLAY-screened peptide P2808 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCAGAGGGGACGAGCTTTTGGTCTTAGAATGCCATCGATGCTAGCCCCATCTGTATTTAAC PQRGRAFGLRMPSMLAPSVFN -1.591 0.015781 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24459 SRGTPSNAIYFHLLRDQSLN 20 SLAY-screened peptide P2809 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCGCGTGGTACGCCCAGTAACGCCATCTATTTTCACCTTTTGCGTGACCAGTCCCTCAACTAA SRGTPSNAIYFHLLRDQSLN* -1.591 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24460 AHD 3 SLAY-screened peptide P2810 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCCCATGATTAGCTCACGACTCACAATTACCAGTACACTCGTTACATTTATTGTCATTATTAA AHD*LTTHNYQYTRYIYCHY* -1.591 0.000177 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24461 TRFWQICTNSNLRTLRWNRP 20 SLAY-screened peptide P2811 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGCGGTTTTGGCAGATCTGCACGAATAGTAACCTTCGTACCCTGCGGTGGAACCGCCCCTAA TRFWQICTNSNLRTLRWNRP* -1.591 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24462 RPTA 4 SLAY-screened peptide P2812 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGCCCACGGCGTAGTCCACCGTTCGGGCCGCGCAGCAGAACCTCCATCCCTCTCACCCGTAA RPTA*STVRAAQQNLHPSHP* -1.591 0.016473 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24463 SKRRFVSTTCPRNSHISDST 20 SLAY-screened peptide P2813 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAAGCGCCGCTTTGTCAGCACGACCTGCCCCCGCAACTCTCATATTTCGGATTCCACGTAA SKRRFVSTTCPRNSHISDST* -1.59 0.018586 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24464 LQYRRHSHLFKGVGLSPSTY 20 SLAY-screened peptide P2814 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCAGTACCGTCGCCACTCCCACTTGTTTAAGGGTGTTGGCTTGTCCCCCTCTACTTACTAA LQYRRHSHLFKGVGLSPSTY* -1.59 0.002677 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24465 NAATDDTLTLRWPSPRPSNT 20 SLAY-screened peptide P2815 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGCCGCCACGGACGATACGCTTACGCTCCGTTGGCCCTCGCCTCGCCCTTCCAATACGTAA NAATDDTLTLRWPSPRPSNT* -1.59 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24466 PFYSETPILTHSCNTLYFAM 20 SLAY-screened peptide P2816 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGTTCTATTCCGAGACCCCCATTTTGACTCACAGTTGCAATACCTTGTATTTCGCCATGTAA PFYSETPILTHSCNTLYFAM* -1.59 0.026751 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24467 HHMTYTDVTNAKCTRLMVVT 20 SLAY-screened peptide P2817 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATCACATGACCTATACTGATGTTACTAATGCCAAGTGCACGCGTTTGATGGTCGTCACCTAA HHMTYTDVTNAKCTRLMVVT* -1.59 0.032556 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24468 SWTTPKGLSCRPGRSYPYS 19 SLAY-screened peptide P2818 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTGGACCACTCCCAAGGGCCTGTCGTGCCGCCCGGGCCGCTCTTATCCGTATAGTTAGTAA SWTTPKGLSCRPGRSYPYS** -1.59 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24469 CPTDATLTPISHNS 14 SLAY-screened peptide P2819 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCCCACTGATGCGACTCTCACTCCCATTAGCCATAATTCTTAGATGAATTGGCCTATCTAA CPTDATLTPISHNS*MNWPI* -1.59 5e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24470 PIGHIWNGSTIVFHNCRFGS 20 SLAY-screened peptide P2820 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCATTGGCCATATCTGGAATGGTTCTACTATCGTCTTTCATAACTGCCGCTTTGGGAGTTAA PIGHIWNGSTIVFHNCRFGS* -1.589 0.001867 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24471 VHPSHGSSSTAHNYTAPRTT 20 SLAY-screened peptide P2821 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTCCACCCGAGTCATGGTAGCTCTTCGACGGCGCACAATTATACCGCTCCGCGGACTACTTAA VHPSHGSSSTAHNYTAPRTT* -1.589 0.008466 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24472 FMPVLLILFPLTCPCTRHQS 20 SLAY-screened peptide P2822 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTATGCCGGTGCTCCTTATCCTCTTTCCCCTGACCTGTCCTTGCACGCGCCATCAGAGTTAA FMPVLLILFPLTCPCTRHQS* -1.588 0.000482 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24473 NFYIAEPRKCHGFLLNMRGS 20 SLAY-screened peptide P2823 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAACTTTTACATCGCGGAGCCTCGCAAGTGTCATGGTTTCCTCCTCAATATGCGTGGCTCTTAA NFYIAEPRKCHGFLLNMRGS* -1.588 0.001563 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24474 SKSPPNYCCP 10 SLAY-screened peptide P2824 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCAAGTCGCCTCCGAACTACTGTTGCCCCTAGTACCGCGACGCGAATGACCTGTTGAGGTAA SKSPPNYCCP*YRDANDLLR* -1.588 2e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24475 QDGYTSSEDQSSIYRHNLKN 20 SLAY-screened peptide P2825 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGGACGGTTACACGTCTAGTGAGGATCAGTCTAGTATTTACCGCCACAACCTTAAGAATTAA QDGYTSSEDQSSIYRHNLKN* -1.588 0.024643 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24476 QNK 3 SLAY-screened peptide P2826 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGAACAAGTAGTATTGCACTTATGCGATTTTGTTTAACATTTATAACCACATACTTAGTAAC QNK*YCTYAILFNIYNHILSN -1.588 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24477 PLRDFLIRRLIGVTSRRHNT 20 SLAY-screened peptide P2827 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCCTCCGTGACTTCCTTATTCGGCGCCTTATTGGCGTGACCAGTAGGCGGCACAATACTTAA PLRDFLIRRLIGVTSRRHNT* -1.588 0.003921 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24478 TGLGGLTDYNLLAITYDCLL 20 SLAY-screened peptide P2828 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGGGTCTTGGGGGGCTTACTGACTACAACCTGCTCGCTATTACTTACGACTGTTTGCTGTAA TGLGGLTDYNLLAITYDCLL* -1.587 0.020983 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24479 FNKWPVCSLHPGRCVNLMTP 20 SLAY-screened peptide P2829 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTAACAAGTGGCCCGTCTGTTCGTTGCATCCTGGCCGTTGTGTGAACCTCATGACCCCTTAA FNKWPVCSLHPGRCVNLMTP* -1.587 0.046316 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24480 SALALCPSVSLSVE 14 SLAY-screened peptide P2830 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTGCACTTGCTTTATGCCCATCGGTGTCCCTGTCAGTTGAGTGACTATTATGCTTATTAACT SALALCPSVSLSVE*LLCLLT -1.587 0.0001 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24481 FAARVVVCGPTNNTAVSIRI 20 SLAY-screened peptide P2831 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTCGCGGCGCGCGTGGTCGTCTGTGGTCCCACCAACAACACCGCCGTCTCCATTCGGATCTAA FAARVVVCGPTNNTAVSIRI* -1.587 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24482 IMTMSEYSFNSSVHSNHYYLT 21 SLAY-screened peptide P2832 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTATGACTATGTCGGAGTATTCGTTCAACAGTTCCGTCCATTCGAACCACTATTATTTAACT IMTMSEYSFNSSVHSNHYYLT -1.587 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24483 KPAAEPPIVNGGNNYSRQYS 20 SLAY-screened peptide P2833 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAAGCCGGCTGCTGAGCCGCCCATTGTGAATGGCGGTAACAACTACTCGCGGCAGTATTCTTAA KPAAEPPIVNGGNNYSRQYS* -1.586 0.000179 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24484 MLWWTHKYYDWLA 13 SLAY-screened peptide P2834 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATGCTGTGGTGGACCCATAAGTATTATGATTGGCTTGCGTAGTCCTACCGTGGCCGCTAGTAA MLWWTHKYYDWLA*SYRGR** -1.586 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24485 NDATIGGETVSPRVHGNNSI 20 SLAY-screened peptide P2835 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATGACGCCACCATTGGGGGCGAGACTGTTTCGCCTAGGGTTCATGGGAATAACAGTATTTAA NDATIGGETVSPRVHGNNSI* -1.586 0.02355 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24486 PWCPASQINHFPNNRGATST 20 SLAY-screened peptide P2836 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGGTGTCCTGCGTCTCAGATTAATCACTTTCCTAATAACAGGGGCGCTACCTCCACCTAA PWCPASQINHFPNNRGATST* -1.586 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24487 INPIDRHTCDNPLYYIISHF 20 SLAY-screened peptide P2837 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAATCCGATTGACAGGCACACCTGCGATAATCCCCTTTACTACATTATCAGTCACTTCTAA INPIDRHTCDNPLYYIISHF* -1.585 0.011167 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24488 ITIFTRSDFNHRTSYALIPA 20 SLAY-screened peptide P2838 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTACCATTTTCACTCGCTCCGATTTCAATCATCGCACTAGTTACGCTCTCATCCCCGCCTAA ITIFTRSDFNHRTSYALIPA* -1.585 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24489 QRVCPKAPRIRAPPHTLHTW 20 SLAY-screened peptide P2839 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCGGGTCTGTCCGAAGGCTCCGCGTATCCGTGCCCCGCCTCACACTCTGCACACTTGGTAA QRVCPKAPRIRAPPHTLHTW* -1.585 0.009614 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24490 YLSGLRRFYYIWRYLGKTIS 20 SLAY-screened peptide P2840 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATATCTCAGCGGCCTTAGGCGGTTTTATTATATCTGGCGTTACCTCGGGAAGACTATCAGTTAA YLSGLRRFYYIWRYLGKTIS* -1.585 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24491 SSRTSGTIENTRHPLTIIVR 20 SLAY-screened peptide P2841 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTTCGCGTACCTCGGGTACTATTGAGAACACTCGTCATCCCTTGACCATTATTGTCAGGTAA SSRTSGTIENTRHPLTIIVR* -1.585 8.7e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24492 SSVNLTALFTVNSGNTCSVR 20 SLAY-screened peptide P2842 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTTCCGTTAATCTGACTGCCCTGTTCACCGTGAATAGCGGTAACACCTGCTCGGTTCGTTAA SSVNLTALFTVNSGNTCSVR* -1.585 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24493 PSRSVNHYDRIDFCIHRAII 20 SLAY-screened peptide P2843 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTCGCGCTCCGTTAACCATTACGATCGTATCGACTTCTGCATCCACCGTGCCATTATCTAA PSRSVNHYDRIDFCIHRAII* -1.585 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24494 TNRLPETCHHYCVHPRYLKN 20 SLAY-screened peptide P2844 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACGAACCGTCTGCCTGAGACCTGTCATCATTACTGTGTCCACCCCCGGTACCTCAAGAATTAA TNRLPETCHHYCVHPRYLKN* -1.584 0.010822 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24495 CSPFHTDLFTLL 12 SLAY-screened peptide P2845 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTCGCCCTTTCACACTGACCTTTTTACGCTCCTTTAGCCTGTTCGCCTGAATTGTAATTAA CSPFHTDLFTLL*PVRLNCN* -1.584 0.00156 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24496 LSHPAPQPSSDRSNQLEVPY 20 SLAY-screened peptide P2846 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGAGCCACCCTGCCCCTCAGCCTAGCAGCGATCGCTCGAATCAGCTCGAGGTTCCGTATTAA LSHPAPQPSSDRSNQLEVPY* -1.584 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24497 CHKTSPKGEHDISYTQTPHH 20 SLAY-screened peptide P2847 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTCACAAGACCTCGCCCAAGGGTGAGCACGATATCAGTTACACGCAGACCCCCCACCACTAA CHKTSPKGEHDISYTQTPHH* -1.584 0.000255 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24498 SSVCRPFSQFMYAHVNSFAV 20 SLAY-screened peptide P2848 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCTAGTGTCTGCCGTCCTTTTAGCCAGTTCATGTACGCTCATGTCAATTCCTTCGCTGTCTAA SSVCRPFSQFMYAHVNSFAV* -1.584 0.000349 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24499 LS 2 SLAY-screened peptide P2849 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTAGCTAGCCTGATTACAATCAGAGTGTGAATTTGTACTTGCTTTTCATGGATGTTTAGTAA LS*PDYNQSVNLYLLFMDV** -1.583 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24500 YTYCTFTSTHTVICYFPTKR 20 SLAY-screened peptide P2850 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACACGTACTGCACCTTTACCTCTACGCACACTGTGATCTGTTATTTCCCCACGAAGCGTTAA YTYCTFTSTHTVICYFPTKR* -1.583 0.019631 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24501 RPVGPMLCHTEVTMLIIRTN 20 SLAY-screened peptide P2851 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCCTGTCGGTCCTATGCTGTGCCATACTGAGGTCACTATGCTTATCATTCGCACTAATTAA RPVGPMLCHTEVTMLIIRTN* -1.583 0.000971 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24502 LS 2 SLAY-screened peptide P2852 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGTCTTAGCTTGATGATAAGATCGCGTACACTCCGTAGCTGTTTAATAATCGTGATCCCTAA LS*LDDKIAYTP*LFNNRDP* -1.583 0.011105 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24503 SSSRKFRLTSAASAVAPLSN 20 SLAY-screened peptide P2853 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCAGTTCGCGCAAGTTCCGCCTTACGAGCGCGGCCAGTGCCGTGGCCCCGCTGAGCAACTAA SSSRKFRLTSAASAVAPLSN* -1.583 0.008045 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24504 CGIEHNNLTLSPFSGSYHIM 20 SLAY-screened peptide P2854 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGGGATTGAGCATAATAATCTTACCCTCTCCCCGTTCAGCGGCTCTTATCATATTATGTAA CGIEHNNLTLSPFSGSYHIM* -1.582 0.013323 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24505 HLLASLG 7 SLAY-screened peptide P2855 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACCTACTAGCTAGTCTTGGATGAATTAGTCGGCTTTTGGGGTCCTTGACTATGACAGCTAAC HLLASLG*ISRLLGSLTMTAN -1.582 0.045915 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24506 PTLEHKNWFNFKSALHTNNR 20 SLAY-screened peptide P2856 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCGACTCTGGAGCATAAGAATTGGTTTAACTTTAAGTCTGCCTTGCACACTAATAATCGCTAA PTLEHKNWFNFKSALHTNNR* -1.582 1e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24507 TAPSYPHINNHKS 13 SLAY-screened peptide P2857 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGCCCCGTCTTATCCTCATATTAACAATCACAAGAGTTAGGTTCACCACACCATTATCTAA TAPSYPHINNHKS*VHHTII* -1.582 0.004712 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24508 LLLQKAPLASSAHTHCNGPAN 21 SLAY-screened peptide P2858 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTCTGCTCCAGAAGGCTCCTCTCGCCTCCAGCGCGCACACGCATTGCAACGGGCCCGCTAAC LLLQKAPLASSAHTHCNGPAN -1.582 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24509 LVVYLVSLCSTSSTQFVLYL 20 SLAY-screened peptide P2859 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTTGTGGTCTACCTCGTGTCCCTGTGTTCTACTTCTAGCACCCAGTTCGTGTTGTACCTCTAA LVVYLVSLCSTSSTQFVLYL* -1.581 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24510 CRQTHRKAGRPSYS 14 SLAY-screened peptide P2860 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCGGCAGACCCATAGGAAGGCGGGGCGGCCGTCCTATTCCTAGCTGCACATCGATAACTAA CRQTHRKAGRPSYS*LHIDN* -1.581 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24511 APRMHHVTLYPSRDDKFSGT 20 SLAY-screened peptide P2861 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGCCGCGTATGCACCACGTCACCCTTTACCCTTCGCGTGACGATAAGTTTTCCGGGACGTAA APRMHHVTLYPSRDDKFSGT* -1.581 0.009841 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24512 TVFLKAASYLSVRFMTCYTV 20 SLAY-screened peptide P2862 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTGTCTTCCTTAAGGCTGCTAGCTATTTGTCTGTTCGGTTTATGACTTGCTATACCGTTTAA TVFLKAASYLSVRFMTCYTV* -1.581 0.014234 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24513 LVEHMMSKFNNLRNYLRAIS 20 SLAY-screened peptide P2863 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGGTGGAGCATATGATGAGCAAGTTCAATAATCTGAGGAATTATTTGCGGGCGATTTCTTAA LVEHMMSKFNNLRNYLRAIS* -1.581 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24514 PVFANSNSFQSWRRNPGFHC 20 SLAY-screened peptide P2864 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCGTCTTTGCCAACAGTAATAGCTTTCAGAGCTGGCGCCGCAATCCTGGCTTTCACTGCTAA PVFANSNSFQSWRRNPGFHC* -1.581 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24515 TPPNYCAAGFCCPQFPIIGV 20 SLAY-screened peptide P2865 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCCCTCCTAATTACTGCGCGGCCGGCTTCTGTTGCCCTCAGTTTCCGATTATCGGCGTGTAA TPPNYCAAGFCCPQFPIIGV* -1.58 0.03764 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24516 RGPPVTSIALNLLRSTIACSN 21 SLAY-screened peptide P2866 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTGGTCCGCCCGTCACTTCCATTGCCTTGAATCTTCTCAGATCCACAATTGCGTGCTCTAAC RGPPVTSIALNLLRSTIACSN -1.58 0.003196 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24517 HTCTTNPPADLH 12 SLAY-screened peptide P2867 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACACCTGTACCACTAACCCTCCCGCCGACCTTCACTAGCATCGTAGTTGTACGCCTGCTTAA HTCTTNPPADLH*HRSCTPA* -1.58 4.8e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24518 ILCIYRPRRLPSFRIYLGLR 20 SLAY-screened peptide P2868 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCCTGTGCATTTACCGTCCCCGGCGTTTGCCTTCCTTTCGCATCTATCTGGGTCTTCGTTAA ILCIYRPRRLPSFRIYLGLR* -1.58 0.000292 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24519 YLTIYSLPFAMSITNVTLIH 20 SLAY-screened peptide P2869 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACCTTACCATTTATTCTTTGCCCTTTGCGATGTCGATTACTAATGTTACCCTGATTCACTAA YLTIYSLPFAMSITNVTLIH* -1.58 0.000395 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24520 RTSVITNLLVHRYYSTLMPL 20 SLAY-screened peptide P2870 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGCACTTCTGTCATTACCAACCTCCTTGTTCATAGGTATTATTCCACGTTGATGCCGCTCTAA RTSVITNLLVHRYYSTLMPL* -1.58 0.001705 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24521 RYPPASHPRQAVAYSHSRSM 20 SLAY-screened peptide P2871 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTACCCCCCCGCTTCTCATCCGCGCCAGGCCGTCGCTTATTCCCACAGCAGGAGTATGTAA RYPPASHPRQAVAYSHSRSM* -1.58 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24522 ITYGHLACPSVNRRFLTAST 20 SLAY-screened peptide P2872 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATTACGTACGGCCACCTCGCGTGTCCCTCTGTCAATCGTCGGTTCCTCACCGCCTCCACCTAA ITYGHLACPSVNRRFLTAST* -1.579 7.3e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24523 FYALHNHKFIATNKVKTNDS 20 SLAY-screened peptide P2873 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTTTATGCTCTGCACAACCATAAGTTCATCGCTACCAATAAGGTGAAGACCAATGATAGTTAA FYALHNHKFIATNKVKTNDS* -1.579 0.000872 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24524 QRTYRASINFGHSYVCYSPF 20 SLAY-screened peptide P2874 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGCGTACTTACAGGGCGTCCATCAATTTTGGTCACAGCTATGTGTGCTATTCGCCCTTCTAA QRTYRASINFGHSYVCYSPF* -1.579 3e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24525 GEHAY 5 SLAY-screened peptide P2875 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGTGAGCACGCGTACTAGCCTGAGCACTCGTCGAACGTTCAGAAGCGGCTTAGTAGGAGTTAA GEHAY*PEHSSNVQKRLSRS* -1.579 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24526 TSTTFRQPNCTVSLARATLS 20 SLAY-screened peptide P2876 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCTCTACTACGTTCCGTCAGCCTAATTGCACCGTTAGTCTGGCCCGCGCTACGCTGTCTTAA TSTTFRQPNCTVSLARATLS* -1.578 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24527 HNIAYTKKRAGPLRVLCVGSN 21 SLAY-screened peptide P2877 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACACAACATTGCCTACACTAAGAAGCGAGCAGGGCCTTTGCGGGTCCTTTGTGTGGGCAGTAAC HNIAYTKKRAGPLRVLCVGSN -1.578 1.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24528 NISHISKNHS 10 SLAY-screened peptide P2878 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATATCAGTCATATCTCCAAGAATCATTCCTAGATCTGTTGTAGCGGGTTTCCTAACTCCTAA NISHISKNHS*ICCSGFPNS* -1.578 0.004037 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24529 RLRPCDSNSTWWSLNSID 18 SLAY-screened peptide P2879 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACGTCTCCGGCCTTGTGACTCTAATAGCACTTGGTGGAGCCTTAATTCTATCGACTAGGACTAA RLRPCDSNSTWWSLNSID*D* -1.578 0.001922 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24530 TGPYHPLRIIPCSNLYYGIL 20 SLAY-screened peptide P2880 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGGCCCGTATCACCCCCTGAGGATCATCCCCTGTTCGAACCTTTACTACGGGATTCTTTAA TGPYHPLRIIPCSNLYYGIL* -1.577 9e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24531 ATPSGVWPATVLDQSMSPNF 20 SLAY-screened peptide P2881 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGCGACCCCGTCTGGTGTGTGGCCTGCGACCGTGCTTGATCAGTCCATGTCTCCCAACTTTTAA ATPSGVWPATVLDQSMSPNF* -1.577 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24532 YWDHHCDNDSRINRVHMYLL 20 SLAY-screened peptide P2882 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATACTGGGACCACCATTGTGATAACGACAGCCGTATCAACAGGGTTCATATGTATCTTCTGTAA YWDHHCDNDSRINRVHMYLL* -1.577 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24533 CTHAHQRAISTYTI 14 SLAY-screened peptide P2883 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCACGCATGCCCATCAGCGGGCCATCTCGACTTATACCATATGACCACTCTTCTGCTGTAAC CTHAHQRAISTYTI*PLFCCN -1.577 0.000349 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24534 PLPLLYSPPYYSQIIPCSLL 20 SLAY-screened peptide P2884 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTCTGCCCTTGCTGTATTCGCCCCCCTACTACTCCCAGATCATTCCCTGTAGCCTTCTCTAA PLPLLYSPPYYSQIIPCSLL* -1.576 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24535 GTNWSTP 7 SLAY-screened peptide P2885 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGGACCAACTGGTCTACCCCGTAGTTCAGGGCTCACCCTTATTCTAACACTACTAACCCGTAA GTNWSTP*FRAHPYSNTTNP* -1.576 0.026293 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24536 TQCPPYLTCYVPSIVPYCWV 20 SLAY-screened peptide P2886 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCAGTGCCCCCCGTATCTCACCTGCTATGTGCCTAGTATTGTCCCTTACTGCTGGGTGTAA TQCPPYLTCYVPSIVPYCWV* -1.576 0.027274 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24537 LNRPFNPWVPCFMV 14 SLAY-screened peptide P2887 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCAATCGCCCGTTCAATCCTTGGGTTCCCTGTTTTATGGTTTAGCTTTATTGCAGTGACTAA LNRPFNPWVPCFMV*LYCSD* -1.576 0.016877 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24538 HSGRDSMFDD 10 SLAY-screened peptide P2888 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACATTCCGGCCGCGATTCCATGTTTGACGACTAGATTGACGTGTCTATCGCTCACGTTGGTAAC HSGRDSMFDD*IDVSIAHVGN -1.576 0.000112 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24539 CCPFFTNSPYDHPCIVYARA 20 SLAY-screened peptide P2889 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCTGCCCCTTTTTTACCAACTCTCCTTACGATCACCCTTGCATTGTTTACGCTCGTGCCTAA CCPFFTNSPYDHPCIVYARA* -1.576 0.007485 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24540 PTKPPTPTHLILTYPC 16 SLAY-screened peptide P2890 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTACCAAGCCGCCTACGCCCACCCACCTGATTCTTACCTATCCCTGTTAGAATGTTTGTTAA PTKPPTPTHLILTYPC*NVC* -1.575 0.005307 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24541 CAPPDIIHLFNATGSNFPEL 20 SLAY-screened peptide P2891 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTGCGCCTCCCGATATTATCCATCTGTTCAACGCTACTGGCAGCAATTTTCCGGAGCTTTAA CAPPDIIHLFNATGSNFPEL* -1.575 2.9e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24542 NYITAQHAVSERPVRVMLHT 20 SLAY-screened peptide P2892 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAATTACATCACCGCCCAGCATGCTGTTAGCGAGCGCCCGGTTCGGGTTATGCTTCATACGTAA NYITAQHAVSERPVRVMLHT* -1.574 0.002355 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24543 PSIKCYDQPMHNRPLIYYSA 20 SLAY-screened peptide P2893 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCTAGTATCAAGTGTTATGACCAGCCCATGCATAACCGCCCCCTTATTTACTACAGTGCCTAA PSIKCYDQPMHNRPLIYYSA* -1.574 0.001006 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24544 LVPIWRRCSRRSAVNTCISL 20 SLAY-screened peptide P2894 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTCGTCCCCATTTGGCGTCGTTGTTCCCGTCGCTCTGCTGTCAATACCTGCATTTCTCTGTAG LVPIWRRCSRRSAVNTCISL* -1.574 6.2e-05 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24545 VFPMNDTSHHHLRPVPGGWL 20 SLAY-screened peptide P2895 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTTTTTCCGATGAACGATACTTCCCACCATCATCTGCGTCCGGTGCCTGGCGGGTGGCTCTAA VFPMNDTSHHHLRPVPGGWL* -1.574 0.000143 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24546 QNFRVPCIHCIVSFLDSFYE 20 SLAY-screened peptide P2896 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACAGAATTTTCGTGTCCCTTGCATTCATTGCATTGTTAGTTTCCTCGACAGCTTTTATGAGTAA QNFRVPCIHCIVSFLDSFYE* -1.574 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24547 CLLS 4 SLAY-screened peptide P2897 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCCTGCTGTCGTAGTCCTCTTCGAACGGGTGTCGTAACTTCCGCCTCCCTCCCATTCTTTAA CLLS*SSSNGCRNFRLPPIL* -1.574 0.000106 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24548 DPISSIYASQVRLRTVLGSSN 21 SLAY-screened peptide P2898 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGACCCCATTAGCAGCATTTACGCCAGCCAGGTACGATTGCGGACCGTCTTGGGGTCCAGTAAC DPISSIYASQVRLRTVLGSSN -1.574 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24549 GMCSAYRTYLV 11 SLAY-screened peptide P2899 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGGCATGTGTAGTGCTTATAGGACCTACCTGGTGTAGAATGGCGTTCCCTTGACCATTAAGTAA GMCSAYRTYLV*NGVPLTIK* -1.573 0.000269 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24550 LTATRPYRPP 10 SLAY-screened peptide P2900 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACTGACCGCGACGCGCCCGTATCGCCCGCCGTAGCGCAACAGCGTTAGGGTTAAGTACACTTAA LTATRPYRPP*RNSVRVKYT* -1.573 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24551 LYRNSVLLKASL 12 SLAY-screened peptide P2901 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATTGTATCGGAATAGCGTCCTTCTTAAGGCTAGCTTGTAGTGGCACTTTTGTTATATCGGCTAA LYRNSVLLKASL*WHFCYIG* -1.573 0.026418 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24552 SFNAYSRVYSDCYYIHGVAG 20 SLAY-screened peptide P2902 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGCTTCAATGCTTATTCGCGTGTGTACTCCGATTGCTACTATATCCACGGCGTCGCTGGCTAA SFNAYSRVYSDCYYIHGVAG* -1.573 0.022601 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24553 IRNTFLLRRDRMPGAYDSLP 20 SLAY-screened peptide P2903 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCAGGAATACGTTCCTTTTGCGGCGGGATCGCATGCCGGGGGCCTATGACTCTCTTCCGTAA IRNTFLLRRDRMPGAYDSLP* -1.573 0.003593 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24554 SLKPTGTTRACFQPVISLA 19 SLAY-screened peptide P2904 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATCCCTCAAGCCTACGGGTACGACTCGGGCTTGCTTCCAGCCCGTCATTAGCCTGGCGTAGTAA SLKPTGTTRACFQPVISLA** -1.573 0.000465 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24555 VAPGAGFPIRDMVHSRTSHI 20 SLAY-screened peptide P2905 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAGTGGCCCCTGGGGCCGGGTTTCCCATTCGGGATATGGTTCACTCCCGCACCTCCCATATTTAA VAPGAGFPIRDMVHSRTSHI* -1.573 0.004701 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24556 RSRGVINKTYSRNAHVHFRN 20 SLAY-screened peptide P2906 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGTCGCGCGGGGTTATTAACAAGACGTATTCGCGCAACGCCCATGTTCACTTTCGCAATTAA RSRGVINKTYSRNAHVHFRN* -1.573 1e-06 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24557 SRPDGARHNFHSRPIL 16 SLAY-screened peptide P2907 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGTCGGCCTGACGGGGCCCGCCATAATTTCCATAGTCGCCCAATACTCTGAGTAAGTCGACCT SRPDGARHNFHSRPIL*VSRP -1.573 0.008011 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24558 TVTLGRAIPLPANNP 15 SLAY-screened peptide P2908 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACCGTGACGCTCGGCCGTGCCATTCCCCTTCCTGCCAACAACCCTTAGGATTGCTACCTGTAA TVTLGRAIPLPANNP*DCYL* -1.573 0.023582 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24559 PCLMCQSYAPGVGCIFLATL 20 SLAY-screened peptide P2909 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGACCCTGCCTCATGTGTCAGTCCTACGCCCCCGGTGTTGGTTGCATTTTCCTGGCCACCCTGTAA PCLMCQSYAPGVGCIFLATL* -1.572 0.035008 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24560 CEWDLPCRRRAHTDRKAGNP 20 SLAY-screened peptide P2910 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGCGAGTGGGACCTGCCTTGTCGGCGTCGTGCTCATACCGACCGCAAGGCGGGTAACCCTTAA CEWDLPCRRRAHTDRKAGNP* -1.572 0.022776 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24561 IGGYSPSDATYTIVCFMPNGT 21 SLAY-screened peptide P2911 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAATCGGAGGATACAGCCCGTCCGATGCTACTTACACTATTGTTTGCTTTATGCCCAACGGTACG IGGYSPSDATYTIVCFMPNGT -1.572 0.026346 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24562 TLKKIKRVVHYLCDWCNHHW 20 SLAY-screened peptide P2912 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAACTCTCAAGAAGATCAAGCGCGTTGTGCACTACTTGTGCGACTGGTGTAACCACCATTGGTAA TLKKIKRVVHYLCDWCNHHW* -1.572 0.0 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24563 CFLSSLIPSRPSLDIGCAHE 20 SLAY-screened peptide P2913 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGATGTTTTCTTTCTTCTCTCATCCCTAGCAGGCCCTCCCTGGACATTGGTTGCGCTCATGAGTAA CFLSSLIPSRPSLDIGCAHE* -1.572 0.001701 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated. Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively." DRAMP24564 RAHYIYSTANSL 12 SLAY-screened peptide P2914 Screening by SLAY Anti-recombinant genetically engineered E. coli Linear 29307492 Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries Candidate AMP GGAGGAAGGGCCCACTATATTTACTCCACCGCCAACAGTCTCTAGTTTACTTTCGAGACGTTGGACTAA RAHYIYSTANSL*FTFETLD* -1.572 0.007449 "Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with