General Information
-
DRAMP ID
- DRAMP03600
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Peptide Name
- Human beta-defensin 4 (hBD-4, BD-4; Beta-defensin 104; Human, mammals, animals)
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Source
- Homo sapiens (Human)
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Family
- Belongs to the beta-defensin family
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Gene
- DEFB104A
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Sequence
- EFELDRICGYGTARCRKKCRSQEYRIGRCPNTYACCLRKWDESLLNRTKP
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Sequence Length
- 50
-
Protein Existence
- Transcript level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- Gram-positive bacterium: Staphylococcus carnosus TM300 (MIC=4.5 µg/ml);
- Gram-negative bacteria: Escherichia coli BL21 (MIC>100 µg/ml), Pseudomonas aeruginosa (MIC=4.1 µg/ml).
- Yeast: Saccharomyces cerevisiae ATCC9763 (MIC>100 µg/ml).
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- Free
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C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys8 and Cys36,Cys15 and Cys29,Cys19 and Cys37.
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Stereochemistry
- L
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Structure
- Bridge
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- 5KI9
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Predicted Structure
- There is no predicted structure for DRAMP03600.
Physicochemical Information
-
Formula
- C254H410N82O74S6
Absent Amino Acids
- HMV
Common Amino Acids
- R
Mass
- 5988.91
PI
- 9.27
Basic Residues
- 12
Acidic Residues
- 6
Hydrophobic Residues
- 10
Net Charge
- +6
-
Boman Index
- -171.04
Hydrophobicity
- -1.008
Aliphatic Index
- 50.8
Half Life
-
- Mammalian:1 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 10345
Absorbance 280nm
- 211.12
Polar Residues
- 19
DRAMP03600
Comments Information
Function
- Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
Tissue specificity
- High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
Induction
- Antimicrobial activity is decreased when the sodium chloride concentration is increased.
PTM
- Contains three disulfide bonds (By similarity).
Literature Information
- ·Literature 1
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Title
- Human beta-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity.
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Pubmed ID
- 11481241
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Reference
- FASEB J. 2001 Aug;15(10):1819-1821.
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Author
- García JR, Krause A, Schulz S, Rodríguez-Jiménez FJ, Klüver E, Adermann K, Forssmann U, Frimpong-Boateng A, Bals R, Forssmann WG.