General Information

    • DRAMP ID

    • DRAMP00126

    • Peptide Name

    • Plantaricin E (PlnE; Bacteriocin)

    • Source

    • Lactobacillus plantarum (Gram-positive bacteria)

    • Family

    • Belongs to the class IIb bacteriocin

    • Gene

    • plnE

    • Sequence

    • FNRGGYNFGKSVRHVVDAIGSVAGIRGILKSIR

    • Sequence Length

    • 33

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Pediococcus pentosaceus Pac 1.0 (MIC=5 nM), Lactobacillus plantarum 965 (MIC=7 nM), Lactobacillus casei subsp.casei NCDO 161 (MIC=20 nM), Lactobacillus casei NCDO 2713 (MIC=150 nM), Lactobacillus sakei NCDO 2714 (MIC=4 nM), Lactobacillus viridescens NCDO 1655 (MIC=2 nM), Pediococcus pentosaceus NCDO 990 (MIC=30 nM), Lactobacillus sakei 706 (MIC=100 nM), Pediococcus acidilactici CH (MIC=10 nM), Lactobacillus curvatus 89 (MIC=5 nM). [PlnE:PlnF=1:1]

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • There is a possible hydrogen bond/salt bridge between Asp17 and Arg26

    • Stereochemistry

    • L

    • Structure

    • Alpha helix (3 helices; 19 residues)

    • Structure Description

    • In DPC micelles, PlnE has an N-terminal alpha-helix (residues 10-21), and a C-terminal alpha-helix-like structure (residues 25-31). Both helices in plnE are amphiphilic, while the helix in plnF is polar in its N-terminal half and amphiphilic in its C-terminal half. PlnE has two GxxxG motifs which are putative helix-helix interaction motifs, one at residues 5 to 9 and one at residues 20 to 24.

    • Helical Wheel Diagram

    • DRAMP00126 helical wheel diagram

    • PDB ID

    • 2JUI resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP00126.

Physicochemical Information

    • Formula

    • C158H259N51O42

    • Absent Amino Acids

    • CEMPQTW

    • Common Amino Acids

    • G

    • Mass

    • 3545.11

    • PI

    • 11.57

    • Basic Residues

    • 7

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +6

    • Boman Index

    • -51.8

    • Hydrophobicity

    • 0.067

    • Aliphatic Index

    • 100.3

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 46.56

    • Polar Residues

    • 12

DRAMP00126

DRAMP00126 chydropathy plot

Comments Information

    • Comment

    • No comments found on DRAMP database

Literature Information

  • ·Literature 1

    • Title

    • The gene encoding plantaricin A, a bacteriocin from Lactobacillus plantarum C11, is located on the same transcription unit as an agr-like regulatory system.

    • Reference

    • Appl Environ Microbiol. 1994 Jan;60(1):160-166.

    • Author

    • Diep DB, Håvarstein LS, Nissen-Meyer J, Nes IF.
  • ·Literature 2

    • Title

    • Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin plantaricin EF.

    • Reference

    • Biochim Biophys Acta. 2008 Nov;1784(11):1711-1719.

    • Author

    • Fimland N, Rogne P, Fimland G, Nissen-Meyer J, Kristiansen P.E.