• • DRAMP ID

  • DRAMP00191

• • Peptide Name

  • Microcin J25 (MccJ25; Bacteriocin)

• • Source

  • Escherichia coli AY25 (Gram-negative bacteria)

• • Family

  • Belongs to the class I microcin

• • Gene

  • mcjA

• • Sequence

  • GGAGHVPEYFVGIGTPISFYG

• • Sequence Length

  • 21

• • UniProt Entry

  • Q9X2V7

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram-

• • Target Organism

  • • Gram-negative bacteria: Escherichia coli BM21 (MIC=0.08 µg/ml), E. coli AB1133 (MIC=0.02 µg/ml), E. coli RYC816 (MIC=0.04 µg/ml), E. coli AY29 (MIC=0.04 µg/ml), Salmonella species, Shigella flexneri.

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Cyclization of a N-terminal between Gly and Glu

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Beta strand

• • Structure Description

  • MccJ25 does not have a head-to-tail cyclic structure but rather a side chain to backbone cyclization between Glu8 and the N-terminus. This creates an embedded ring that is threaded by the C-terminal tail of the molecule, forming a noose-like feature. The three-dimensional structure deduced from NMR data suggests that slippage of the noose is prevented by two aromatic residues flanking the embedded ring.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 1Q71,1PP5 resolved by NMR.
• 1Q71-> 
  1PP5-> 

• Predicted Structure

• There is no predicted structure for DRAMP00191.

DRAMP00191

• • Function

  • Microcin J25 is a 21 amino acid bacterial peptide that has potent antibacterial activity against Gram-negative bacteria, resulting from its interaction with RNA polymerase.

• • Induction

  • At the onset of stationary growth phase.

• • PTM

  • Isoglutamyl glycine isopeptide between G1 and E8.

• ·Literature 1

• • Title

  • Microcin 25, a novel antimicrobial peptide produced by Escherichia coli.

• • Pubmed ID

  • 1429464

• • Reference

  • J Bacteriol. 1992 Nov;174(22):7428-7435.

• • Author

  • Salom³n RA, Far­as RN.

• ·Literature 2

• • Title

  • The cyclic structure of microcin J25, a 21-residue peptide antibiotic from Escherichia coli.

• • Pubmed ID

  • 10092860

• • Reference

  • Eur J Biochem. 1999 Feb;259(3):747-755.

• • Author

  • Blond A, P©duzzi J, Goulard C, Chiuchiolo MJ, Barth©l©my M, Prigent Y, Salom³n RA, Far­as RN, Moreno F, Rebuffat S.

• ·Literature 3

• • Title

  • Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone.

• • Pubmed ID

  • 14531690

• • Reference

  • J Am Chem Soc. 2003 Oct 15;125(41):12464-74.

• • Author

  • Rosengren KJ, Clark RJ, Daly NL, Göransson U, Jones A, Craik DJ.

• ·Literature 4

• • Title

  • Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot.

• • Pubmed ID

  • 14531661

• • Reference

  • J Am Chem Soc. 2003 Oct 15;125(41):12382-3.

• • Author

  • Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH.