General Information

    • DRAMP ID

    • DRAMP00204

    • Peptide Name

    • Thiocillin GE37468 (Antibiotic GE37468; Bacteriocin)

    • Source

    • Streptomyces sp. (Gram-positive bacteria)

    • Family

    • Belongs to the thiocillin family

    • Gene

    • getA

    • Sequence

    • STNCFCYICCSCSS

    • Sequence Length

    • 14

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Bacillus subtilis (MIC=0.047 µg/ml), Staphylococcus aureus (MRSA) (MIC=0.047 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Cyclization

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • ①GE37468 has a macrocyclic ring composed of 10 residues (Ser1 to Cys10). ②Cys6 converts to a thiazoline while four other Cys residues (Cys4, Cys9, Cys10 and Cys12) are fully desaturated to thiazoles in GE37468. ③Ile8 converts to β-methyl-δ-hydroxy-proline(mhP) through a predicted tandem double hydroxylation/cyclization mechanism.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00204 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00204.

Physicochemical Information

    • Formula

    • C58H85N15O22S5

    • Absent Amino Acids

    • ADEGHIKLMQRVW

    • Common Amino Acids

    • C

    • Mass

    • 1504.7

    • PI

    • 5.23

    • Basic Residues

    • 0

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 1

    • Net Charge

    • 0

    • Boman Index

    • -13.57

    • Hydrophobicity

    • 0.357

    • Aliphatic Index

    • 0

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1740

    • Absorbance 280nm

    • 133.85

    • Polar Residues

    • 12

DRAMP00204

Comments Information

    • Function

    • Has bacteriocidal activity against both aerobic and anaerobic Gram-positive bacteria. Has poor activity against Gram-negative bacteria, with the exception of B.fragilis. Inhibits bacterial protein biosynthesis by acting on elongation factor Tu (

Literature Information

  • ·Literature 1

    • Title

    • Identification of the thiazolyl peptide GE37468 gene cluster from Streptomyces ATCC 55365 and heterologous expression in Streptomyces lividans.

    • Reference

    • Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):13053-13058.

    • Author

    • Young TS, Walsh CT.