General Information

    • DRAMP ID

    • DRAMP00750

    • Peptide Name

    • Defensin-like protein 2 (Dm-AMP2; Plant defensin)

    • Source

    • Dahlia merckii (Bedding dahlia)

    • Family

    • Belongs to the DEFL family

    • Gene

    • Not found

    • Sequence

    • EVCEKASKTWSGNCGNTGHC

    • Sequence Length

    • 20

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal

    • Target Organism

      • Medium A: Botrytis cinerea (IC50=10 µg/ml), Cladosporium sphaerospermum (IC50=3 µg/ml), Fusarium culmorum (IC50=5 µg/ml), Leptosphaeria maculans (IC50=1 µg/ml), Penicillium digitatum (IC50=2 µg/ml), Trichoderma viride (IC50>100 µg/ml), Septoria tritiei (IC50=1 µg/ml), Verticilium albo-atrum (IC50=2 µg/ml). NOTE: Medium A = 1/2 strength potato dextrose broth.
      • Medium B: Botrytis cinerea (IC50>100 µg/ml), Cladosporium sphaerospermum (IC50=12 µg/ml), Fusarium culmorum (IC50=12 µg/ml), Leptosphaeria maculans (IC50=20 µg/ml), Penicillium digitatum (IC50=50 µg/ml), Trichoderma viride (IC50>100 µg/ml), Septoria tritiei (IC50=2 µg/ml), Verticilium albo-atrum (IC50>100 µg/ml).
      • NOTE: Medium B = Medium A supplemented with 1 mM CaCl2 and 50 mM KCI.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • cell membrabce

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00750 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00750.

Physicochemical Information

    • Formula

    • C84H131N27O31S3

    • Absent Amino Acids

    • DFILMPQRY

    • Common Amino Acids

    • CG

    • Mass

    • 2111.31

    • PI

    • 6.82

    • Basic Residues

    • 3

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 3

    • Net Charge

    • +1

    • Boman Index

    • -39.76

    • Hydrophobicity

    • -0.83

    • Aliphatic Index

    • 19.5

    • Half Life

      • Mammalian:1 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5625

    • Absorbance 280nm

    • 296.05

    • Polar Residues

    • 12

DRAMP00750

DRAMP00750 chydropathy plot

Comments Information

    • Comment

    • No comments found on DRAMP database

Literature Information

  • ·Literature 1

    • Title

    • A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species.

    • Reference

    • FEBS Lett. 1993 Feb 1;316(3):233-240.

    • Author

    • Terras FR, Torrekens S, Van Leuven F, Osborn RW, Vanderleyden J, Cammue BP, Broekaert WF.
  • ·Literature 2

    • Title

    • Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae.

    • Reference

    • FEBS Lett. 1995 Jul 17;368(2):257-262.

    • Author

    • Osborn RW, De Samblanx GW, Thevissen K, Goderis I, Torrekens S, Van Leuven F, Attenborough S, Rees SB, Broekaert WF.