• • DRAMP ID

  • DRAMP00764

• • Peptide Name

  • Piceain 1 (Plants)

• • Source

  • Picea sitchensis

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • KSLRPRCWIKIKFRCKSLKF

• • Sequence Length

  • 20

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=9.38 µg/ml), S. aureus ATCC 43300 (MIC=9.38 µg/ml);
    • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=18.75 µg/ml), Escherichia coli ML-35P (MIC=18.75 µg/ml), Escherichia coli 08040722 (CI) (MIC=75 µg/ml), Escherichia coli 08A866 (CI) (MIC=37.5 µg/ml), Pseudomonas aeruginosa PA01 (MIC=9.38 µg/ml), Pseudomonas aeruginosa ATCC 27853 (MIC=9.38 µg/ml), Pseudomonas aeruginosa 08031014 (CI) (MIC=37.5 µg/ml).
    • Yeast: Candida albicans ATCC 20032 (MIC=9.38 µg/ml).

• • Hemolytic Activity

  • • [Ref.21644248] At the concentration of 0.0625 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 0.7% & 1.5%; At the concentration of 0.125 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 0.8% & 7.2%; At the concentration of 0.25 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 5.9%&7.8%; At the concentration of 0.5 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 7.6% & 9.5%; At the concentration of 1 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 7.8% & 57.1%.

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • There is one intra-molecular disulfide bridge formed by Cys7 and Cys15.

• • Stereochemistry

  • L

• • Structure

  • Beta strand (CD)

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP00764_predicted_structure.pdb to download.

DRAMP00764

• • Function

  • Active against both Gram-postive and Gram-negative bacteria, fungi. It showed weak hemolysis activity against human and rabbit red cells.

• • PTM

  • Contains one disulfide bond.

• ·Literature 1

• • Title

  • Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis.

• • Pubmed ID

  • 21644248

• • Reference

  • J Pept Sci. 2011 Sep;17(9):627-631.

• • Author

  • Liu R, Mu L, Liu H, Wei L, Yan T, Chen M, Zhang K, Li J, You D, Lai R.