General Information

    • DRAMP ID

    • DRAMP00795

    • Peptide Name

    • Cliotide T1 (cT1; Plant defensin)

    • Source

    • Clitoria ternatea (Butterfly pea)

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GIPCGESCVFIPCITAAIGCSCKSKVCYRN

    • Sequence Length

    • 30

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anti-cancer

    • Target Organism

      • [Ref.21596752]Gram-positive bacteria: Staphylococcus aureus ATCC12600, Enterococcus faecalis ATCC 47077 (less active);
      • Gram-negative bacteria: Escherichia coli ATCC 700926 (MIC=1.1 µM), Pseudomonas aeruginosa ATCC 39018 (MIC=2.7 µM), Klebsiella pneumonia ATCC 13883 (MIC=4.7 µM).

    • Hemolytic Activity

      • [Ref.21596752] HD50 = 7.1 μM against human red blood cells

    • Cytotoxicity

      • [Ref.21596752] IC50=0.6 µM against HeLa cells.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • There are three disulfide bonds between Cys4 and Cys20, Cys8 and Cys22, Cys13 and Cys27.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00795 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C132H216N36O39S6

    • Absent Amino Acids

    • DHLMQW

    • Common Amino Acids

    • C

    • Mass

    • 3123.74

    • PI

    • 8.33

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • -7.52

    • Hydrophobicity

    • 0.657

    • Aliphatic Index

    • 78

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1865

    • Absorbance 280nm

    • 64.31

    • Polar Residues

    • 15

DRAMP00795

DRAMP00795 chydropathy plot

Comments Information

    • Function

    • Cliotide T1 shows stronger antimicrobial activity against the Gram-negative bacteria than the Gram-positive bacteria. It also has hemolytic activity against human type A erythrocytes (HD50=7.1 µM) and cytotoxicity against HeLa Cells (IC50=0.6 µM).

    • [Note

    • HD50 refers to the peptide concentration that causes 50% lysis of red blood cells; IC50 refers to the peptide concentration that causes 50% death of HeLa cells].

Literature Information

  • ·Literature 1

    • Title

    • Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family.

    • Reference

    • J Biol Chem. 2011 Jul 8;286(27):24275-24287.

    • Author

    • Nguyen GK, Zhang S, Nguyen NT, Nguyen PQ, Chiu MS, Hardjojo A, Tam JP.