General Information

    • DRAMP ID

    • DRAMP00804

    • Peptide Name

    • Cycloviolacin-O1 (Plant defensin)

    • Source

    • Viola odorata (Sweet violet)

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GIPCAESCVYIPCTVTALLGCSCSNRVCYN

    • Sequence Length

    • 30

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Insecticidal

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.20575512] Cytotoxicity on the human lymphoma cell line U-937 GTB(IC50 around 1 µM).

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys4 and Cys21; Cys8 and Cys23; Cys13 and Cys28.

    • Stereochemistry

    • L

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00804 helical wheel diagram

    • PDB ID

    • 1NBJ resolved by NMR.
  • 1NBJ-> 

    • Predicted Structure

    • There is no predicted structure for DRAMP00804.

Physicochemical Information

    • Formula

    • C131H211N35O42S6

    • Absent Amino Acids

    • DFHKMQW

    • Common Amino Acids

    • C

    • Mass

    • 3140.69

    • PI

    • 5.96

    • Basic Residues

    • 1

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • 0

    • Boman Index

    • -5.65

    • Hydrophobicity

    • 0.747

    • Aliphatic Index

    • 87.67

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 115.69

    • Polar Residues

    • 17

DRAMP00804

DRAMP00804 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism. It shows haemolytic activity.

    • PTM

    • This is a cyclic peptide which contains three disulfide bonds 4-21; 8-23; 13-28.

Literature Information

  • ·Literature 1

    • Title

    • A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability..

    • Reference

    • Biochem J. 2006 Nov 15;400(1):1-12.

    • Author

    • Ireland DC, Colgrave ML, Craik DJ.
  • ·Literature 2

    • Title

    • Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif.

    • Reference

    • J Mol Biol. 1999 Dec 17;294(5):1327-1336.

    • Author

    • Craik DJ, Daly NL, Bond T, Waine C.
  • ·Literature 3

    • Title

    • Twists, knots, and rings in proteins. Structural definition of the cyclotide framework.

    • Reference

    • J Biol Chem. 2003 Mar 7;278(10):8606-8616.

    • Author

    • Rosengren KJ, Daly NL, Plan MR, Waine C, Craik DJ.