General Information

    • DRAMP ID

    • DRAMP01097

    • Peptide Name

    • Bombinin-like peptide 1 (toads, amphibians, animals)

    • Source

    • Bombina orientalis (Oriental fire-bellied toad)

    • Family

    • Belongs to the bombinin family

    • Gene

    • Not found

    • Sequence

    • GIGASILSAGKSALKGLAKGLAEHFAN

    • Sequence Length

    • 27

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial

    • Target Organism

    • [Ref.1744108] 82% inhibition Neisseria meningitidis 118V C (MIC=20 µg/ml), 98% inhibition Neisseria meningitidis 15240 IAI(MIC=20 µg/ml), 85% inhibition Neisseria meningitidis 126E IC1(MIC=20 µg/ml), 98% inhibition Neisseria gonorrhoeoe F62 (MIC=20 µg/ml), 96% inhibition Neisseria Iactamica 15323(MIC=20 µg/ml), 100% inhibition Neisseria Iactamica 15215A(MIC=20 µg/ml), 98% inhibition Neisseria cinerea 15461 (MIC=20 µg/ml), Bacillus megaterium BmII(MIC=0.3 µM), Staphylococcus aureus Cowan1 (MIC=3.4 µM), Escherichia coli D21 (MIC=1.7 µM), Escherichia coli D22(MIC=1.5 µM), Yersinia pseudotubercolosis (MIC=0.8 µM), Pseudomonas aeruginosa ATCC 15692(MIC=6.3 µM), Candida albicans(MIC=0.4 µM).

    • Hemolytic Activity

      • [Ref.1744108] No significant hemolytic activity against human red blood cell.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • These peptides are cationic but otherwise differ considerably in such basic featuresa st heir size (Mr ~2300-4000), presence of disulfide bonds, and structural motifs.

    • Helical Wheel Diagram

    • DRAMP01097 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C115H193N33O34

    • Absent Amino Acids

    • CDMPQRTVWY

    • Common Amino Acids

    • A

    • Mass

    • 2582

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • 3.1

    • Hydrophobicity

    • 0.426

    • Aliphatic Index

    • 108.89

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 9

DRAMP01097

DRAMP01097 chydropathy plot

Comments Information

    • Function

    • Has antimicrobial activity, but no significant hemolytic activity. Preference on killing Gram-negative non-enteric bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Bombinin-like peptides with antimicrobial activity from skin secretions of the Asian toad, Bombina orientalis.

    • Reference

    • J Biol Chem. 1991 Dec 5;266(34):23103-23111.

    • Author

    • Gibson BW, Tang DZ, Mandrell R, Kelly M, Spindel ER.