General Information

    • DRAMP ID

    • DRAMP01174

    • Peptide Name

    • Ocellatin-4 (Frogs, amphibians, animals)

    • Source

    • Leptodactylus ocellatus (Argus frog) (Leptodactylus macrosternum)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLLDFVTGVGKDIFAQLIKQI

    • Sequence Length

    • 21

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.17884127]Gram-positive bacterium: Staphylococcus aureus ATCC 25923 (MIC=64 µM);
      • Gram-negative bacterium: Escherichia coli ATCC 25922 (MIC=64 µM).

    • Hemolytic Activity

      • [Ref.17884127] HC50 = 14.3 μM against human O+ red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01174 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C107H175N25O29

    • Absent Amino Acids

    • CEHMNPRSWY

    • Common Amino Acids

    • GIL

    • Mass

    • 2275.72

    • PI

    • 5.96

    • Basic Residues

    • 2

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 11

    • Net Charge

    • 0

    • Boman Index

    • 6

    • Hydrophobicity

    • 0.81

    • Aliphatic Index

    • 143.81

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP01174

DRAMP01174 chydropathy plot

Comments Information

    • Function

    • Has hemolytic activity against human erythrocytes (HC50=14.3 µM). Has antibacterial activity.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

Literature Information

  • ·Literature 1

    • Title

    • Purification, characterization and homology analysis of ocellatin 4, a cytolytic peptide from the skin secretion of the frog Leptodactylus ocellatus.

    • Reference

    • Toxicon 2007; 50: 1095-1104.

    • Author

    • Nascimento A et al Castro MS.