• • DRAMP ID

  • DRAMP01258

• • Peptide Name

  • Dermadistinctin-L (DD L; Frogs, amphibians, animals)

• • Source

  • Phyllomedusa distincta (Monkey frog)

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • ALWKTLLKNVGKAAGKAALNAVTDMVNQ

• • Sequence Length

  • 28

• • UniProt Entry

  • P83639

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiprotozoal

• • Target Organism

  • • [Ref.10477123]Gram-positive bacteria: Staphylococcus aureus (MIC=1.3 µM), Enterococcus faecalis (MIC=10 µM);
    • Gram-negative bacteria: Pseudomonas aeruginosa (MIC=40 µM), Escherichia coli (MIC=2.5 µM);
    • [Ref.17442605]Fungi: Candida tropicalis (MIC=10.9 µM), Candida guilliermondii (MIC=21.8 µM), Candida albicans (MIC=21.8 µM), Candida albicans ATCC 1023 (MIC=10.9 µM).

• • Hemolytic Activity

  • • [Ref:10477123]Show some hemolytic starting at 12.5 μM against human blood

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • liposomes

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Free

• • Stereochemistry

  • L

• • Structure

  • Alpha helix

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP01258_predicted_structure.pdb to download.

DRAMP01258

• • Function

  • Also has antiprotozoal activity against Trypanosoma cruzi. Decreases viability of murine peritoneal cells. Fuses to, and disrupts liposomes. Show little hemolytic activity against human red blood cells.

• • Tissue specificity

  • Expressed by the skin glands.

• ·Literature 1

• • Title

  • Antimicrobial peptides from the Brazilian frog Phyllomedusa distincta.

• • Pubmed ID

  • 10477123

• • Reference

  • Peptides. 1999;20(6):679-686.

• • Author

  • Batista CV, da Silva LR, Sebben A, Scaloni A, Ferrara L, Paiva GR, Olamendi-Portugal T, Possani LD, Bloch C Jr.

• ·Literature 2

• • Title

  • Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta. Anti-Trypanosoma cruzi activity without cytotoxicity to mammalian cells.

• • Pubmed ID

  • 12379643

• • Reference

  • J Biol Chem. 2002 Dec 20;277(51):49332-49340.

• • Author

  • Brand GD, Leite JR, Silva LP, Albuquerque S, Prates MV, Azevedo RB, Carregaro V, Silva JS, Sá VC, Brandão RA, Bloch C Jr.

• ·Literature 3

• • Title

  • Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta: liposomes fusion and/or lysis investigated by fluorescence and atomic force microscopy.

• • Pubmed ID

  • 17409003

• • Reference

  • Comp Biochem Physiol A Mol Integr Physiol. 2008 Nov;151(3):329-335.

• • Author

  • Silva LP, Leite JR, Brand GD, Regis WB, Tedesco AC, Azevedo RB, Freitas SM, Bloch C Jr.

• ·Literature 4

• • Title

  • Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta: Secondary structure, antimicrobial activity, and mammalian cell toxicity.

• • Pubmed ID

  • 17442605

• • Reference

  • Comp Biochem Physiol A Mol Integr Physiol. 2008 Nov;151(3):336-343.

• • Author

  • Leite JR, Brand GD, Silva LP, Kückelhaus SA, Bento WR, Araújo AL, Martins GR, Lazzari AM, Bloch C Jr.