General Information

    • DRAMP ID

    • DRAMP01346

    • Peptide Name

    • Prepromelittin-related peptide (Frogs, amphibians, animals)

    • Source

    • Rana sakuraii (Japanese brown frog)

    • Family

    • Not found

    • Gene

    • prMRP

    • Sequence

    • VIGSILGALASGLPTLISWIKNR

    • Sequence Length

    • 23

    • Protein Existence

    • Homology

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacterium: Escherichia coli (MIC=25 µM);
      • Gram-positive bacterium: Staphylococcus aureus (MIC=6 µM).
      • Yeast: Candida albicans (MIC=25 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01346 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C110H187N29O29

    • Absent Amino Acids

    • CDEFHMQY

    • Common Amino Acids

    • IL

    • Mass

    • 2379.87

    • PI

    • 11

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +2

    • Boman Index

    • 12.29

    • Hydrophobicity

    • 0.97

    • Aliphatic Index

    • 156.96

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 250

    • Polar Residues

    • 8

DRAMP01346

DRAMP01346 chydropathy plot

Comments Information

    • Function

    • Has antibacterial against Gram-positive and Gram-negative bacteria. Also has antifungal activity against C. albicans.

Literature Information

  • ·Literature 1

    • Title

    • Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii.

    • Reference

    • Peptides. 2007;28(3):505-514.

    • Author

    • Suzuki H, Iwamuro S, Ohnuma A, Coquet L, Leprince J, Jouenne T, Vaudry H, Taylor CK, Abel PW, Conlon JM.
  • ·Literature 2

    • Title

    • Evidence that the genes encoding the melittin-related peptides in the skins of the Japanese frogs Rana sakuraii and Rana tagoi are not orthologous to bee venom melittin genes: developmental- and tissue-dependent gene expression.

    • Reference

    • Peptides. 2007 Oct;28(10):2061-2068.

    • Author

    • Suzuki H, Conlon JM, Iwamuro S.