General Information

    • DRAMP ID

    • DRAMP01359

    • Peptide Name

    • Ranalexin-1G (Frogs, amphibians, animals)

    • Source

    • Rana grylio (North American pig frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • FLGGLMKIIPAAFCAVTKKC

    • Sequence Length

    • 20

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Homology

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacterium: Escherichia coli (MIC=9 µM);
      • Gram-positive bacterium: Staphylococcus aureus (MIC=18 µM).
      • Yeast: Candida albicans (MIC=70 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys14 and Cys20)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys14 and Cys20.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01359 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C98H163N23O22S3

    • Absent Amino Acids

    • DEHNQRSWY

    • Common Amino Acids

    • AK

    • Mass

    • 2111.69

    • PI

    • 9.39

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +3

    • Boman Index

    • 22.68

    • Hydrophobicity

    • 1.195

    • Aliphatic Index

    • 107.5

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.58

    • Polar Residues

    • 5

DRAMP01359

DRAMP01359 chydropathy plot

Comments Information

    • Function

    • Has growth-inhibitory activity against the gram-positive bacterium Staphylococcus aureus, the gram-negative bacterium Escherichia coli and the yeast, Candida albicans.

Literature Information

  • ·Literature 1

    • Title

    • Purification and characterization of antimicrobial and vasorelaxant peptides from skin extracts and skin secretions of the North American pig frog Rana grylio.

    • Reference

    • Regul Pept. 2000 Jun 30;90(1-3):53-60.

    • Author

    • Kim JB, Halverson T, Basir YJ, Dulka J, Knoop FC, Abel PW, Conlon JM.