General Information

    • DRAMP ID

    • DRAMP01438

    • Peptide Name

    • Nigrocin-2JDa (Frogs, amphibians, animals)

    • Source

    • Odorrana jingdongensis (Jingdong frog) (Rana jingdongensis)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GIFGKILGAGKKVLCGLSGLC

    • Sequence Length

    • 21

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.22917879]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=16 µM), Staphylococcus aureus CIB 85462 (MIC=8 µM);
      • Gram-negative bacteria: Escherichia coli ATCC 25992 (MIC=16 µM), Escherichia coli CIB 84492 (MIC=16 µM);
      • Yeast: Candida albicans (MIC=63 µM).

    • Hemolytic Activity

      • [Ref:22917879] It has 80.3 ± 5.5% hemolytic activity at 128 μg/ml against rabbit red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys15 and Cys21)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys15 and Cys21.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01438 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C92H160N24O23S2

    • Absent Amino Acids

    • DEHMNPQRTWY

    • Common Amino Acids

    • G

    • Mass

    • 2034.55

    • PI

    • 9.39

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +3

    • Boman Index

    • 26.5

    • Hydrophobicity

    • 1.1

    • Aliphatic Index

    • 130

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.25

    • Polar Residues

    • 9

DRAMP01438

DRAMP01438 chydropathy plot

Comments Information

    • Function

    • Shows antibacterial activity against both Gram-positive and Gram-negative bacteria and against the fungus C. albicans. Has hemolytic activity against rabbit erythrocytes.

    • PTM

    • contains one disulfide bond 15-21.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from the skin of the Asian frog, Odorrana jingdongensis: De novo sequencing and analysis of tandem mass spectrometry data.

    • Reference

    • J Proteomics. 2012 Oct 22;75(18):5807-5821.

    • Author

    • Liu J, Jiang J, Wu Z, Xie F.