General Information

    • DRAMP ID

    • DRAMP01440

    • Peptide Name

    • Nigrocin-2LVb (Frogs, amphibians, animals)

    • Source

    • Odorrana livida (Chinese Odorrana frogs)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GILSGILGMGKKLVCGLSGLC

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus (MIC=50 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=15 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys15 and Cys21)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys15 and Cys21.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01440 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C88H159N23O24S3

    • Absent Amino Acids

    • ADEFHNPQRTWY

    • Common Amino Acids

    • G

    • Mass

    • 2019.55

    • PI

    • 8.9

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • 31.13

    • Hydrophobicity

    • 1.3

    • Aliphatic Index

    • 143.81

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.25

    • Polar Residues

    • 10

DRAMP01440

DRAMP01440 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activity against the Gram-positive bacterium S. aureus and the Gram-negative bacterium E. coli.

Literature Information

  • ·Literature 1

    • Title

    • Nigrocin-2 peptides from Chinese Odorrana frogs--integration of UPLC/MS/MS with molecular cloning in amphibian skin peptidome analysis.

    • Reference

    • FEBS J. 2010 Mar;277(6):1519-1531.

    • Author

    • Wang L, Evaristo G, Zhou M, Pinkse M, Wang M, Xu Y, Jiang X, Chen T, Rao P, Verhaert P, Shaw C.