General Information

    • DRAMP ID

    • DRAMP01490

    • Peptide Name

    • Esculentin-2A (Frogs, amphibians, animals)

    • Source

    • Rana esculenta (European Edible frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GILSLVKGVAKLAGKGLAKEGGKFGLELIACKIAKQC

    • Sequence Length

    • 37

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.8163497]Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=0.2 µM), Staphylococcus aureus Cowan 1 (MIC=0.8 µM);
      • Gram-negative bacteria: Escherichia coli D21 (MIC=0.4 µM), Pseudomonas aeruginosa ATCC15692 (MIC=3.5 µM).

    • Hemolytic Activity

      • [Ref:8163497] Not found in the literature

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys31 and Cys37)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys31 and Cys37.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01490 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C168H295N45O44S2

    • Absent Amino Acids

    • DHMNPRTWY

    • Common Amino Acids

    • GK

    • Mass

    • 3713.59

    • PI

    • 9.7

    • Basic Residues

    • 7

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 17

    • Net Charge

    • +5

    • Boman Index

    • 12.12

    • Hydrophobicity

    • 0.543

    • Aliphatic Index

    • 124.05

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.47

    • Polar Residues

    • 10

DRAMP01490

DRAMP01490 chydropathy plot

Comments Information

    • Function

    • Shows antibacterial activity against representative Gram-negative and Gram-positive bacterial species.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from skin secretions of Rana esculenta. Molecular cloning of cDNAs encoding esculentin and brevinins and isolation of new active peptides.

    • Reference

    • J Biol Chem. 1994 Apr 22;269(16):11956-11961.

    • Author

    • Simmaco M, Mignogna G, Barra D, Bossa F.