General Information

    • DRAMP ID

    • DRAMP01491

    • Peptide Name

    • Esculentin-1B (Frogs, amphibians, animals)

    • Source

    • Rana esculenta (European Edible frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GIFSKLAGKKLKNLLISGLKNVGKEVGMDVVRTGIDIAGCKIKGEC

    • Sequence Length

    • 46

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria: Escherichia coli D21 (MIC=0.2 µM), Pseudomonas aeruginosa ATCC15692 (MIC=0.7 µM);
      • Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=0.1 µM), Staphylococcus aureus cowan I (MIC=0.4 µM).
      • Fungi: Candida albicans (MIC=0.5 µM), Saccharomyces cerevisiae (MIC=0.9 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys40 and Cys46)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys40 and Cys46.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01491 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C212H371N59O60S3

    • Absent Amino Acids

    • HPQWY

    • Common Amino Acids

    • GK

    • Mass

    • 4802.82

    • PI

    • 9.63

    • Basic Residues

    • 9

    • Acidic Residues

    • 4

    • Hydrophobic Residues

    • 17

    • Net Charge

    • +5

    • Boman Index

    • -28.64

    • Hydrophobicity

    • 0.204

    • Aliphatic Index

    • 114.35

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 2.78

    • Polar Residues

    • 15

DRAMP01491

DRAMP01491 chydropathy plot

Comments Information

    • Comment

    • No comments found on DRAMP database

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from skin secretions of Rana esculenta. Molecular cloning of cDNAs encoding esculentin and brevinins and isolation of new active peptides.

    • Reference

    • J Biol Chem. 1994 Apr 22;269(16):11956-11961.

    • Author

    • Simmaco M, Mignogna G, Barra D, Bossa F.