General Information

    • DRAMP ID

    • DRAMP01546

    • Peptide Name

    • Nigroain-K1 (Frogs, amphibians, animals)

    • Source

    • Rana nigrovittata (Black-striped frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • SLWETIKNAGKGFIQNLDKIR

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.19778602]Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=9.38 µg/ml), Bacillus subtilis (MIC=21.88 µg/ml);
      • Gram-negative bacterium: Escherichia coli ML-35P (MIC=37.50 µg/ml);
      • Yeast: Candida albicans ATCC2002 (MIC=32.74 µg/ml).

    • Hemolytic Activity

      • [Ref:19778602]22.49 ± 4.8% hemolytic activity at 100 µg/ml against rabbit red cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01546 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C110H179N31O31

    • Absent Amino Acids

    • CHMPVY

    • Common Amino Acids

    • IK

    • Mass

    • 2431.82

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • -38.29

    • Hydrophobicity

    • -0.533

    • Aliphatic Index

    • 97.62

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 275

    • Polar Residues

    • 6

DRAMP01546

DRAMP01546 chydropathy plot

Comments Information

    • Function

    • Has antibacterial and antifungal activity. Also has hemolytic activity against rabbit red cells.

Literature Information

  • ·Literature 1

    • Title

    • Peptidomics and genomics analysis of novel antimicrobial peptides from the frog, Rana nigrovittata.

    • Reference

    • Genomics. 2010 Jan;95(1):66-71.

    • Author

    • Ma Y, Liu C, Liu X, Wu J, Yang H, Wang Y, Li J, Yu H, Lai R.