General Information

    • DRAMP ID

    • DRAMP02246

    • Peptide Name

    • Ranatuerin-1C (Ranatuerin 1C; Frogs, amphibians, animals)

    • Source

    • Rana clamitans (Green frog) (Lithobates clamitans)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

    • Gene

    • Not found

    • Sequence

    • SMLSVLKNLGKVGLGLVACKINKQC

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.10822101] Gram-positive bacterium: Staphylococcus aureus (MIC=55 μM);
      • Gram-negative bacterium: Escherichia coli (MIC=1.5 μM).
      • Yeast: Candida albicans (MIC=58 μM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys19 and Cys25)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys19 and Cys25.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02246 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C114H206N32O31S3

    • Absent Amino Acids

    • DEFHPRTWY

    • Common Amino Acids

    • L

    • Mass

    • 2617.27

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +4

    • Boman Index

    • 3.36

    • Hydrophobicity

    • 0.636

    • Aliphatic Index

    • 132.4

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.21

    • Polar Residues

    • 9

DRAMP02246

DRAMP02246 chydropathy plot

Comments Information

    • Function

    • Antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Purification and characterization of antimicrobial peptides from the skin of the North American green frog Rana clamitans.

    • Reference

    • Peptides. 2000 Apr;21(4):469-476.

    • Author

    • Halverson T, Basir YJ, Knoop FC, Conlon JM.